key: cord-0774013-3ferumgg
authors: Mendes-de-Almeida, Daniela P.; Martins-Gonçalves, Remy; Morato-Santos, Renata; de Carvalho, Gustavo Adolpho C.; Martins, Silas A.; Palhinha, Lohanna; Sandim, Vanessa; Avvad-Portari, Elyzabeth; Bozza, Fernando A.; Monteiro, Robson Q.; Bozza, Patrícia T.; Kurtz, Pedro
title: Intracerebral hemorrhage associated with vaccine-induced thrombotic thrombocytopenia following ChAdOx1 nCOVID-19 vaccine in a pregnant woman
date: 2021-07-15
journal: Haematologica
DOI: 10.3324/haematol.2021.279407
sha: 019ece30606cfa51ad0e10445e5835a6a9a854c2
doc_id: 774013
cord_uid: 3ferumgg

Not available.

Intracerebral hemorrhage associated with vaccine-induced thrombotic thrombocytopenia following ChAdOx1 nCOVID-19 vaccine in a pregnant woman Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread globally, causing significant morbidity and mortality. Therefore, highly effective vaccines play an important role in increasing population immunity and preventing severe disease. The ChAdOx1 nCoV-19 (Oxford-AstraZeneca) vaccine consists of a replicationdeficient chimpanzee adenoviral vector containing the SARS-CoV-2 structural surface glycoprotein spike antigen gene. 1 No signal for increased thrombotic events was detected in clinical trials of this vaccine involving 23,848 participants, 1 which has now been administered to more than 34 million people worldwide. 2 As reported to date, 20.8 million doses of the ChAdOx1 nCoV-19 vaccine have been applied in Brazil. 3 ChAdOx nCoV-19 has been associated with vaccine-induced thrombotic thrombocytopenia (VITT), 4 a rare but serious adverse event. The potential underlying associated mechanism is the formation of anti-heparin/platelet factor 4 (PF4) antibodies, but risk factors other than young age remain unclear. 2, 5 The immune-driven disease resembles heparin-induced thrombocytopenia (HIT) because platelet-activating antibodies recognize multimolecular complexes between cationic PF4 and anionic heparin, but classically there is no heparin exposure in VITT. 5 So far, the incidence of VITT is around one case per 100,000 exposures to the ChAdOx1 nCoV-19 vaccine. 2 Clinically, it is characterized by thrombocytopenia and thrombosis in unusual sites, particularly cerebral venous or splanchnic-vein thrombosis. Cerebral venous sinus thrombosis (CVST), a life-threatening event, was described in 72% of the VITT reports. 6 Clinical trials did not include pregnant women, and the available data came from accidental pregnancies. 7 Pregnant patients with COVID-19 are more likely to die or need intensive care compared with non-pregnant persons of reproductive age. 1 Herein we present a fatal case of a 35-year-old pregnant woman who developed intracerebral hemorrhage in the left temporal lobe associated with VITT 12 days after the off-label ChAdOx1 nCOVID-19 vaccination and we show the characterization of the hemostatic profile. A 35-years-old white pregnant woman at 23 weeks gestation was in prenatal follow-up with normal platelet counts and controlled hypothyroidism due to Hashimoto's disease. She received the first dose of the ChAdOx1 nCov-19 vaccine in late April 2021 (day 0). The next day, she reported having minor symptoms (malaise, chills, tremors, and "cold feeling"). On day 2, she presented a generalized skin rash on her legs, abdomen, and back with spontaneous resolution (see Figure 1A ). Prenatal exams on day 3 identified urinary tract infection caused by Morganella morganii treated with trimethoprim-sulfamethoxazole and 148,000/mm 3 platelets at the routine complete blood count. On day 7, she presented headache, nausea, nonspecific malaise, polaciuria, pain in the lower limbs and hips. Despite having 121,000/mm 3 platelets, she received analgesics and was discharged from the obstetric emergency room. On the following days, she maintained headache and bilateral leg pain, partially responsive to dipyrone. On day 11, she reported a severe headache located in the left maxillary region, and on day 12, she was admitted with 33,000/mm 3 platelets and an excruciating headache. During the computed tomography (CT) exam, she became comatose and underwent endotracheal intubation, which precluded the realization of CT cerebral venography, confirming the suspicion of central venous sinus thrombosis. Brain CT showed a large acute intraparenchymal hematoma in the temporal lobe, insula, and temporoparietal transition of the left cerebral hemisphere measuring approximately 10.1x5.4x5.5 cm. The hematoma was surrounded by vasogenic edema with midline shift to the right, herniation of the uncus, marked compressive effect on the midbrain, and subtotal collapse of the supratentorial ventricular system ( Figure  1B ). The patient received platelet concentrate transfusion and underwent urgent neurosurgery for hematoma drainage and decompressive craniectomy. After receiving critical care procedures, obstetric ultrasound detected fetal death. There were no platelet clumps, signs of erythrocyte fragmentation, or blast cells on blood film. She 3025 haematologica | 2021; 106 (11) Figure 1C to F). Platelet activation and functional assay were performed as described. 9 ,10 As shown in Figure 2 , platelets from the patient exhibited signs of activation. Increased CD63 surface expression, a marker of dense granules secretion, was observed when compared to control (Figure 2A and B) . Moreover, although platelet surface expression of CD62p was not different between the patient and controls, elevated levels of circulating sCD62p and platelet-derived microparticles expressing CD62p were observed in the patient compared to controls ( Figure 2C to E). Functional activity of patient plasma to activate platelets from healthy volunteers was demonstrated in Figure 2F to H. Patient plasma trigger increased surface expression of CD62p, CD63, and the release of platelet-derived microparticles expressing CD62p. Conversely, plasma-induced platelet activation was inhibited by a high concentration of heparin (100 IU/mL). We present the first report of VITT following ChAdOx1 nCOVID-19 vaccination in a pregnant woman. Despite the lack of image verification of cerebral thrombosis, clinical features were suggestive. Initial brain CT may be negative for CVST in about 30% of cases. 11 Besides that, thrombosis was observed in placental vessels. The constellation of signs and symptoms suggests VITT diagnosis complicated by intracranial hemorrhage with a fatal outcome both to fetus and mother. Three independent descriptions of 39 persons presenting VITT after vaccination with ChAdOx1 nCoV-19 revealed a vast majority being women younger than 50 years of age and some of them receiving estrogen-replacement therapy or oral contraceptives. Nevertheless, most of the participants did not have preexisting risk factors for thrombosis. 2 

thrombocytopenia, increased frequency of disseminated intravascular coagulation, and atypical thrombotic events. Serum from these patients shows a strong reactivity on the PF4-heparin ELISA and activates platelets in the presence and absence of heparin, but a high concentration of heparin completely inhibits the effect, 5 as occurred in our case. In addition, we observed platelet activation confirmed by increased CD63 surface expression, circulating sCD62p levels, and platelet-derived microparticles expressing CD62p. Interestingly, we identified an elevation in the patient´s plasma of IL-1b and IL-18 compared to controls. Of note, IL-1b and IL-18 are central mediators of inflammation and thrombosis, released upon inflammasome activation. 12 Indeed, inflammasome activation and IL-1b release play multiple functions that may favor vein and arterial thrombosis including the induction of procoagulant activity, the promotion of leukocyte adhesion to vascular endothelial cells, induction of neutrophil extracellular traps, and pyroptosis. 13 Future studies should address whether or not this is also observed in other VITT cases and participate in the disease pathophysiology. Stroke affects 30 per 100,000 pregnancies, with ischemia, CVST, and hemorrhage causing roughly equal numbers and the highest risk in peripartum and postpartum. 14 However, it is unclear if the pregnancy itself could increase the risk for thrombotic events following ChAdOx1 nCov-19 vaccination with the formation of anti-PF4 antibodies. Another element that should be pointed out, in this case, is the history of thyroiditis. Hypothyroidism is an underestimated risk 3027 haematologica | 2021; 106(11) factor for CVST, although most of patients have additional risk factors, suggesting a multifactorial hypercoagulability. 11 This case report draws attention to this possible severe vaccine side effect among pregnant women and the challenges related to an early diagnosis. When symptomatic, pregnant women experience difficulties diagnosing VITT due to avoidance of screening methods involving radiation and presenting features possibly related to pregnancy, such as thrombocytopenia. The presence of persistent headache and thrombocytopenia within 30 days of vaccination should raise the suspicion of VITT. D-dimer, fibrinogen, and imaging exams help to guide prompt treatment, preventing a rapidly progressive disease. 5 At least five countries had instituted limitations primarily based on age on which patients should receive the ChAdOx1 nCoV-19 vaccine, 2 and Brazil recently temporarily suspended the administration of this vaccine in pregnant women. 15 Those either affected by VITT or under investigation for this complication should not receive a second ChAdOx1 nCoV-19 vaccine. Physicians should have a low threshold for recognizing VITT signs and symptoms and requesting ELISA testing for PF4-polyanion antibodies and confirmatory functional tests. Although rare, VITT is a new phenomenon with devastating effects for otherwise healthy young adults, and its association with COVID-19 vaccination requires a thorough risk-benefit analysis especially for pregnant women.

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Daniela P. Mendes-de-Almeida, 1, 2, 3 Remy Martins-Gonçalves, 4 Renata Morato-Santos, 5 Gustavo Adolpho C. de Carvalho, 6, 7 Silas A. Martins, 6, 7 Lohanna Palhinha, 4 Vanessa Sandim, 8 Elyzabeth Avvad-Portari, 9, 10