key: cord-0767897-pvspyqoh authors: Matsunami, Masatoshi; Suzuki, Tomo; Terao, Toshiki; Kuji, Hiroshi; Matsue, Kosei title: Immune response to SARS-CoV-2 vaccination among renal replacement therapy patients with CKD: a single-center study date: 2021-11-08 journal: Clin Exp Nephrol DOI: 10.1007/s10157-021-02156-y sha: 8a45eee02ac6ca9891fdfa0d6c7058400a41d86e doc_id: 767897 cord_uid: pvspyqoh nan excluded a patient on treatment for immunosuppression. Due to Japan's vaccine delivery systems, group vaccination was conducted mostly with two doses of Comirnaty COVID-19 Vaccine (BioNTech-Pfizer BNT162b2). The SARS-CoV-2-specific antibodies were evaluated and compared to that of 38 controls (a population expected to have optimal antibody response) who were volunteers that met the criteria of over 60 years of age with no evidence of kidney failure, active cancer or an ongoing treatment for immunosuppression. They were selected over a 4-month period of time by consecutive sampling from patients attending our gastroenterology outpatient clinic. Serum samples were tested for SARS-CoV-2 antibodies using the commercially available test system Elecsys ® Anti-SARS-CoV-2 S RUO (Roche Diagnostics, Basel, Switzerland), measuring immunoglobulin G (IgG) levels against SARS-CoV-2 spike S1 subunit. The mean age in the KTx recipients was 59 (38-81) years and lower compared to the controls, HD, and PD patients with a mean age of 74.5 (63-92), 72.9 (60-91), and 75.0 (61-95) years, respectively. Male sex was less prevalent in the controls (47.3%) compared with the HD (65.3%), PD (62.9%), and KTx recipient (57.1%) patients. The median time on dialysis was 6.0 and 2.6 years in the HD and PD patients. In the KTx recipients, the median time after kidney transplantation was 4.0 years. After the second vaccination, anti-SARS-CoV-2-S (Spike) IgG levels were found to be positive (> 0.8 U/ml) in all 38 controls (100%), 77 of 78 HD patients (98.7%), and 26 of 27 PD patients (96.2%), but only 10 of 21 KTx recipients (47.6%). The median S-IgG level (IQR) was 447 (IQR 308.2-1067), 200.5 (IQR 116.2-376.5), 233 (IQR 164.5-689), and 0.7 (IQR 0.4-34.7) in the control, HD, PD, and KTx recipient groups, respectively. The median S-IgG levels were significantly lower in the HD patients (p < 0.0001) and KTx recipients (p < 0.0001) compared with controls ( Fig. 1 ). In contrast, there was no significant difference between the PD patients and controls (Fig. 1) . No life-threatening allergic reaction or other side-effect was observed post-vaccination. The KTx recipients were on a uniform immunosuppressive therapy with calcineurin inhibitor (CNI) in 20 of 21 patients, mycophenolate mofetil (MMF) or mizoribine (MZB) in 19 of 21, and steroid in 21 of 21. Recently, studies have shown that development of antibodies due to the second dose of COVID-19 mRNA vaccines was found to be higher at 95.4% in HD patient [3] . Similarly, another study reported that antibody response in PD patients after a second dose was higher at 95.6% [5] . In comparison, our study has shown similar results with 98.7 and 96.2% antibody positivity in HD and PD patients, respectively. Contrastingly, the antibody response to second dose COVID-19 mRNA vaccines in KTx recipients was reported to be poor in several studies, ranging from 48 to 58.8%. [2, 4] . Risk factors for inadequate antibody response were older age, less time after transplant, number of used immunosuppressants, and type of immunosuppressant (antimetabolite MMF or co-stimulation blocker Belatacept) [1, 2, 4] . Although no threshold has been established for protective immunity, we found low antibody positivity (47.6%) and titers due to second doses of the COVID-19 mRNA vaccines in KTx recipients. This may be related to the widely described impaired immune response of persistent use of immunosuppressants. Limitation of our study includes small sample size in the KTx recipients, and types, doses, and levels of immunosuppressants were similar, which limits our conclusion on which immunosuppressant causes impaired immune response. In conclusion, the early data that describe the antibody response after the SARS-CoV-2 vaccination suggest the response may be lower (antibody titers) in CKD patients who are receiving RRT compared to healthy patients; however, relatively high antibody positivity were observed in the dialysis patients. The response appears diminished for the KTx recipients and those on immunosuppressive therapy. For the KTx recipients and those with impaired immune responses, alternate vaccination strategies and platforms should be considered. vaccine. SARS-CoV-2 spike protein specific antibody titers were determined using Elecsys ® Anti-SARS-CoV-2 S RUO. Antibody titers > 0.8 U/ml were considered as positive immune response to vaccination. Median antibody titers after second vaccination were significantly higher in the controls compared to hemodialysis patients and kidney transplant recipients. The data were analyzed using Graph-Pad Prism 7.0 (GraphPad Software, San Diego, CA). For comparison of the four groups (non-normally distributed samples), the data were analyzed using the non-parametric Kruskal-Wallis and post hoc Dunn's tests. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 are depicted in the figure Humoral and cellular immunity to SARS-CoV-2 vaccination in renal transplant versus dialysis patients: A prospective, multicenter observational study using mRNA-1273 or BNT162b2 mRNA vaccine Immunogenicity of SARS-CoV-2 BNT162b2 vaccine in solid organ transplant recipients Humoral and cellular responses to mRNA-1273 and BNT162b2 SARS-CoV-2 vaccines administered to hemodialysis patients Low immunization rates among kidney transplant recipients who received 2 doses of the mRNA-1273 SARS-CoV-2 vaccine Antibody response to mRNA SARS-CoV-2 vaccine among dialysis patients-a prospective cohort study Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations The authors would like to thank Dr. So Nakaji