key: cord-0766397-8ejre1vj
authors: Salkeld, Daniel J; Padgett, Kerry A; Jones, James Holland
title: A meta‐analysis suggesting that the relationship between biodiversity and risk of zoonotic pathogen transmission is idiosyncratic
date: 2013-03-11
journal: Ecol Lett
DOI: 10.1111/ele.12101
sha: bec8cbd6bb0b274338fbaf5ce5f5f302dadb7e82
doc_id: 766397
cord_uid: 8ejre1vj

Zoonotic pathogens are significant burdens on global public health. Because they are transmitted to humans from non‐human animals, the transmission dynamics of zoonoses are necessarily influenced by the ecology of their animal hosts and vectors. The ‘dilution effect’ proposes that increased species diversity reduces disease risk, suggesting that conservation and public health initiatives can work synergistically to improve human health and wildlife biodiversity. However, the meta‐analysis that we present here indicates a weak and highly heterogeneous relationship between host biodiversity and disease. Our results suggest that disease risk is more likely a local phenomenon that relies on the specific composition of reservoir hosts and vectors, and their ecology, rather than patterns of species biodiversity.

Zoonotic pathogensdisease agents such as the SARS coronavirus, Lyme disease spirochete or West Nile virus, which are transmitted to humans from non-human animalsare significant burdens on global public health (Jones et al. 2008 ). Zoonotic transmission is inherently a multi-species phenomenon, often also involving vector intermediaries, making an understanding of underlying ecological processes essential for management and control. Indeed, a key question in disease ecology and epidemiology is how to mitigate or ameliorate pathogen transmission from wildlife reservoirs to human populations. The 'dilution effect' hypothesis, which suggests that disease risk will decrease as a result of increased species diversity, offers an intriguing possibility of harnessing conservation initiatives in order to reduce disease risk to human populations (Pongsiri et al. 2009; Keesing et al. 2010 ). Here, we use the inclusive definition of the dilution effect which describes the net effect of species diversity reducing disease risk by any of a variety of mechanisms (Keesing et al. 2006) . For example, Borrelia burgdorferi, the bacterium that causes Lyme disease, is transmitted by ticks (Ixodes spp.), but persists in vertebrate hosts (e.g. white-footed mice, Peromyscus leucopus, Sorex shrews and other species), which differ in the degree to which they can infect ticks (i.e. reservoir competence) (LoGiudice et al. 2003; Brisson et al. 2008) . The risk of human exposure to Lyme disease is therefore strongly influenced by the local abundance and interactions of tick hosts, Borrelia reservoir hosts, and Ixodes ticks. LoGiudice et al. (2003 LoGiudice et al. ( , 2008 have hypothesised that a higher diversity of hosts for ticks could reduce the risk of Lyme disease. Similarly, reservoir competence of mosquito-borne West Nile virus (WNV) varies widely among avian species (Komar et al. 2003) , and higher avian biodiversity (species richness and evenness) has been postulated to reduce WNV risk (e.g. Ezenwa et al. 2006; Swaddle & Calos 2008; Allan et al. 2009 ). Unlike WNV and B. burgdorferi, hantaviruses are generally associated with only a single species of rodent host, and transmission does not involve vectors, but a negative association between host diversity and hantavirus prevalence has nevertheless been proposed via mechanisms such as a higher biodiversity suppressing the encounter rate within a reservoir species, and therefore dampening intraspecific disease transmission (Clay et al. 2009; Dizney & Ruedas 2009; Carver et al. 2011) .

If higher biodiversity reduces the risk of infectious diseases, then the dilution effect hypothesis has an obvious appeal as both conservation and public health agendas can be united in a common purpose: protect biodiversity, while simultaneously reducing deleterious health impacts of zoonotic and wildlife diseases (Pongsiri et al. 2009; Keesing et al. 2010) . Indeed, a recent review concluded that 'overall, despite many remaining questions, preserving intact ecosystems and their endemic biodiversity should generally reduce the prevalence of infectious diseases' (Keesing et al. 2010) .

However, emerging zoonoses involve multi-trophic level interactions that can be complex and variable and will often depend on the local idiosyncrasies of pathogen, host, vector and human ecology, which suggests that the dilution effect may not be a general phenomenon (e.g. LoGiudice et al. 2008; Ogden & Tsao 2009;  These conflicting results about the relationship between biodiversity and disease risk suggest that consequences of conservation programmes for human health may be unpredictable and variable depending on the spatial scale and the ecological particulars of the system in question. If there is an argument to be made for redirecting scarce public health or conservation resources, it is critical to understand whether the relationship between biodiversity and disease risk is as generalisable as has been suggested. However, we are unaware of any formal assessment of the generality of the dilution effect.

Here, we analyse existing literature in a meta-analysis that examines the relationship between biodiversity and zoonotic disease risk. We investigate whether the inclusive definition of the dilution effect that biodiversity is protective against disease riskis generally supported and can be regarded as a useful tool in public health approaches to zoonoses. If there is no straightforward relationship between biodiversity and risk of zoonotic disease, then integrated approaches to disease control may require more detailed understanding of the transmission ecology of specific pathogen, vector and host species.

We aimed to collect and analyse all studies that evaluated links between host biodiversity and disease risk for disease agents that infect humans. There are other studies that ask related questions for non-human diseases, but our primary interest was the possible relationship between public health and wildlife biodiversity. Studies were only selected if they simultaneously provided field-based measures of disease risk (e.g. prevalence of infection in animal, human or vector populations; density of infected animals, humans or vectors) and measures of wildlife biodiversity [e.g. species richness (number of species present) or a diversity index (Shannon's index, Simpson's index, etc.)]. Studies most frequently involved multiple sites across which biodiversity varied, but we also included one study that examined disease prevalence in the presence and absence of a particular species (voles) over time (Carver et al. 2011) , and one study where non-reservoirs were removed (Suz an et al. 2009) . Where studies presented data on different subsets of biodiversity, we used the most complete representations of species richness, as this criterion can be applied consistently across all studies. For example, all bird species combined, rather than separate analyses of passerines or non-passerines (Ezenwa et al. 2006) ; or the 'whole model' rather than separate analyses of small mammals, large mammals or birds (LoGiudice et al. 2008) . For studies that examined multiple measures of disease risk [e.g. seroprevalence and abundance of seropositive animals (Piudo et al. 2011)] , we chose the disease risk measure most consistently used across the study system [e.g. seroprevalence was used in most other hantavirus studies, so seroprevalence was adopted from Piudo et al. (2011) ].

We did not include studies where a proxy was used for biodiversity (e.g. forest fragmentation) or where disease risk was simply inferred (e.g. greater abundance of a particular reservoir host but without direct measures of disease). We did not incorporate studies that relied on county-level health data, because infection dynamics can operate at fine spatial scales and therefore data at coarse county or region levels may not be indicative of local pathogen transmission patterns, and because regional measures of biodiversity are sensitive to sampling effort, so that larger areas tend to report more species that smaller areas, making it difficult to obtain an accurate measure of diversity (Loss et al. 2009; Hamer et al. 2011) .

Studies were identified through a comprehensive search on Web of Science â , using search strings examining disease and biodiversity, with a particular focus on West Nile virus, hantaviruses and Lyme disease risk. These pathogens were chosen because they infect humans and are cited in recent reviews as good examples of disease systems linking changes in biodiversity to adverse impacts upon human health (e.g. Pongsiri et al. 2009; Keesing et al. 2010) . Unpublished data on Sin Nombre virus seroprevalence in California small mammals were provided by California Department of Public Health (see Table 1 ). Also included are two studies of the tick-borne disease agent, Anaplasma phagocytophilum (Foley et al. 2009; unpublished data from Nieto et al. 2007) , and unpublished data on plague (Yersinia pestis) in Colorado prairie dog populations from Stapp 2007 (Table 1 ).

An important finding of our meta-analysis is that the investigations of relationships between biodiversity and zoonotic disease risk is still in its infancy; a systematic literature search revealed only 13 published studies documenting relationships of wildlife biodiversity and zoonotic disease risk (following our criteria), and we were able to augment this with three unpublished data sets. Given the limited data available, conclusions regarding the biodiversity-disease relationship should be regarded with caution. Furthermore, our criteria for inclusion in the meta-analysis resulted in a small sample size and consequent low analytical power. Different selection criteria for the inclusion of studies may result in different conclusions.

Meta-analysis depends on a standardised measure of effect size (Rosenthal & DiMatteo 2001) . This measure is essentially a correlation between a factor (e.g. biodiversity) and a response (e.g. infection prevalence). Most studies do not publish raw numbers but most (though not all) publish values of test statistics (e.g. F, v 2 , t, or r 2 ). For each study, we converted the test statistic for the relationships between biodiversity and disease risk to a standardised correlation-based effect size r (see Rosenthal & DiMatteo 2001) . Where these results were not readily apparent in the published papers, we contacted authors and requested either the raw data or a derived effect size statistic, for example, via simplification of data to a contingency table.

The correlation measure of effect size, r, is heteroscedastic and is frequently transformed using Fisher's Z transformation to equalise variances (Rosenthal & DiMatteo 2001; Chaplin-Kramer et al. 2011) . For effect-size measure r, Fisher's Z is calculated as follows:

Studies where disease risk was lower in treatments of higher biodiversity were assigned a negative value.

We adopted a random-effects model, for which each study is taken to be a sample of a large population of possible studies (Cooper et al. 2009 ). The variance in random-effects specification includes both the error term common to the fixed-effects model but also a term that accounts for between-study heterogeneity. The null hypothesis that this between-study variance is zero can be evaluated with a Cochran's Q, which is distributed as chi-square with k À 1 degrees of freedom. Confidence intervals on overall effect sizes are frequently constructed assuming a normal approximation. However, a number of authors have shown that normal confidence intervals substantially under-estimate the true uncertainty in overall Table 1 Details of studies included in meta-analysis: disease agent, study location, the components of the biodiversity-disease relationship (e.g. species richness, disease seroprevalence, Rosenthal's r, Because of the uneven reporting of test statistics, we also present the published P-values to interpret the statistical strength of the reported relationships between biodiversity and disease risk (Hedges & Olkin 1985) .

We used a funnel plot, which depicts standardised effect size against the study standard error, to search for any evidence of publication bias (Chaplin-Kramer et al. 2011) . If there is no publication bias, effect sizes from studies with larger sample sizes will be near the average, whereas effect sizes from smaller sample sizes will spread on both sides of the average. That is, unbiased data should be shaped like a funnel, whereas biased data will generate an asymmetric funnel plot (Chaplin-Kramer et al. 2011) . We also tested for the effect of potential publication bias by using the trim-and-fill method (Duval & Tweedie 2000a,b) , implemented in the R package meta. This intuitively appealing method allows the imputation of possible missing effect-size estimates based on inferred publication bias. In the case of publication bias, confidence intervals are likely to be systematically under-estimated since the variance of the low-power samples (i.e. the bottom right of our funnel plot) is truncated by the missing samples. In trim-and-fill method, confidence intervals for the overall effect size are recalculated including imputed values. Confidence intervals for the trim-and-fill method are calculated using a normal approximation. A sample not subject to publication bias should show little change in the estimated confidence intervals from the original sample to the augmented sample. The trim-and-fill approach also allows rank-based estimates (R 0 + and L 0 + ) of the probable number of missing studies due to publication bias, rounded to the nearest integer as recommended (Duval & Tweedie 2000a,b) . Because this is a question of publication bias, we calculate this value for both our complete sample (which includes three unpublished studies) and the sub-sample of only published papers (n = 13).

We analysed 16 biodiversity-disease relationships: eight studies on hantaviruses (including one unpublished data set), three studies on West Nile virus, two studies on Lyme disease risk, two studies on A. phagocytophilum (including one unpublished data set) and one study on plague (from an unpublished data set) (Table 1) .

Across all studies, the mean Fisher's Z was À0.138 (95% CI using t-distribution = À0.220 to À0.057; n = 16) from the fixedeffects model. However, the test for heterogeneity in the error models of the individual studies was significant at the P < 0.0001 level (Q = 66.66, d.f. = 15), indicating that the random effects model is more appropriate for the overall mean. The mean effect from the random effects model was À0.2301 (95% CI using t-distribution = À0.470-0.008; n = 16). The 95% confidence interval crosses zero, suggesting a non-significant relationship between measures of biodiversity and zoonotic disease risk (Fig. 1) . The distribution of P-values was uniform (Fig. 2) , suggesting that the relationship between biodiversity and disease risk fails to contradict the null hypothesis overall.

The funnel plot (Fig. 3) provides mixed evidence of publication bias but is nonetheless quite revealing. First, all the strongest negative effects (i.e. those supporting the dilution effect hypothesis) are for studies with quite small sample sizes, and therefore large standard errors. Second, while the effect-sizes for the larger studies do indeed cluster near the overall mean effect size, this mean is quite modestly negative. Finally, there is a pronounced asymmetry to the funnel, with more highly negative results at the lowest sample sizes. This is suggestive that either (1) small studies that do not support the dilution effect hypothesis are not published or (2) that there is unmeasured heterogeneity in the studies. Spearman rank correlations between Fisher's Z and standard error were not statistically significant at the conventional P < 0.05 level: excluding unpublished studies, q = 0.51, P = 0.06; across all studies, q = 0.46, P = 0.11. This last point is suggestive of publication bias favouring negative relationships between biodiversity and disease risk.

The trim and fill analysis uses a normal approximation for the confidence interval of the estimated overall effect size (in contrast to the t-distribution CIs we report above): for the full sample, the fixed-effect model yielded a mean Fisher's Z of À0.138 (95% CI using normal distribution = À0.213 to À0.063; n = 16); for the random-effects model, the mean Fisher's Z was À0.230 (95% CI using normal distribution = À0.450 to À0.010; n = 16) with a significant test of heterogeneity in the error models of the individual studies (P < 0.0001, Q = 66.66, d.f. = 15), indicating that the random effects model is more appropriate for the overall mean. For the sub-sample of published studies, the fixed-effect model yielded a mean Fisher's Z of À0.159 (95% CI using normal distribution = À0.236 to À0.081; n = 13); for the random-effects model, the mean Fisher's Z was À0.331 (95% CI using normal distribution = À0.587-0.075; n = 13) with a significant test of heterogeneity in the error models of the individual studies (P < 0.0001, Q = 62.59, d.f. = 12), indicating that the random effects model is more appropriate for the overall mean.

Imputing the possible missing effect-size estimates using the trim and fill analyses calculates CI intervals that mirror the results found from our analyses of the t-distributions. For the full sample, the trim and fill imputation added one study to the sample. For the fixed-effect model, mean Fisher's Z was À0.129 (95% CI using normal distribution = À0.204 to À0.054; n = 17); and for the random effects model, mean Fisher's Z was À0.175 (95% CI using normal distribution = À0.416-0.066; n = 17). Once again, the test for heterogeneity in the error models of the individual studies was significant at the P < 0.0001 level (Q = 86.21, d.f. = 16), indicating that the random effects model is more appropriate for the overall mean and suggests a non-significant overall effect of biodiversity on disease risk. The rank-based estimates of the probable number of missing studies due to publication bias are R 0 + = 4 and L 0 + = 4, suggesting that four studies are missing from the meta-analysis due to publication bias (Duval & Tweedie 2000a,b) . This represents 25% of the total sample of studies analysed here and 20% of the hypothetical sample. For the sub-sample of published studies, trim and fill analysis imputation added three studies to this sub-sample, and calculated a mean Fisher's Z for the fixed-effect model of À0.117 (95% CI using normal distribution = À0.193 to À0.040; n = 16); and for the random effects model, mean Fisher's Z was À0.105 (95% CI using normal distribution = À0.388-0.179; n = 16). Once again, the test for heterogeneity in the error models of the individual studies was significant at the P < 0.0001 level (Q = 106.99, d.f. = 15), indicating that the random effects model is more appropriate for the overall mean and suggests a non-significant overall effect of biodiversity on disease risk. The rank-based estimates of the probable number of missing studies due to publication bias are R 0 + = 7 and L 0 + = 5, suggesting that between five and seven studies are missing from the published sub-sample due to publication bias (Duval & Tweedie 2000a,b) , representing 38-54% of the total sample of studies analysed here and 31-44% of the hypothetical sample.

Our results are not consistent with the claim that biodiversity provides a general service of reducing the risk of infectious disease. Considering just hantavirus study systems, the mean Fisher's Z-value was À0.1438 (n = 8, 95% t-distribution CI = À0.231-0.057) from the fixed-effects model, with 3/8 studies reporting a statistically significant relationship at P < 0.05 (Table 1 ). The hantavirus studies showed significant evidence for heterogeneity (Q = 52.44, d.f. = 7, P < 0.0001). The mean and 95% CI from the random effects model was À0.325 (À0.668-0.019). Mean Fisher's Z-value s for tick-borne disease agents (A. phagocytophilum and B. burgdorferi combined) was 0.112 (95% t-distribution CI = À0.186-0.410, n = 4; fixed-effects model), with 0/4 studies reporting a statistically significant relationship at P < 0.05 (Table 1) . Mean Fisher's Z-values for West Nile virus was À0.672 (95% t-distribution CI = À1.126-0.272, n = 3), with 1/3 studies reporting a statistically significant relationship at P < 0.05 (Table 1) .

Our meta-analysis of effect sizes and P-values from the published literature on human disease agents provides very weak support, at best, for the dilution effect, and by extension the assertion that the preservation of endemic biodiversity will reduce the prevalence of zoonotic diseases. Although the mean effect size is negative, the confidence interval around the mean crosses zero, so the null hypothesis of no effect cannot be rejected. Furthermore, there is strong evidence for heterogeneity in the effects, suggesting that different processes are acting on the different studies. We conclude that the relationship between biodiversity and zoonotic disease risk is probably idiosyncratic, and that understanding the ecological dynamics of specific disease systems is more important in predicting zoonotic disease risk.

A challenge in assessing the evidence in support of the dilution effect is the differences between disease systems and/or variable measures of disease risk. For example, we used two studies examining Lyme disease: LoGiudice et al. (2008) determined risk by nymphal infection prevalence (NIP, i.e. proportion of ticks infected with B. burgdorferi), whereas Prusinski et al. (2006) measured B. burgdorferi infection prevalence in mammalian hosts. Lyme disease risk can also be measured as density of infected nymphs (DIN), and the implications of the dilution effect are starkly different depending on the risk measure adopted: for instance, a reduction in nymphal infection prevalence associated with increased host biodiversity may be negated if higher host biodiversity increases vector density (Schmidt & Ostfeld 2001; Salkeld & Lane 2010; Wood & Lafferty 2012) , which is why several studies suggest that DIN is a more direct measure of risk to humans. Similarly, in Argentina, host species diversity and evenness was not correlated with Andes virus (a hantavirus) antibody seroprevalence in the reservoir hostthe long-tailed colilargo, Oligoryzomys longicaudatusbut there was a correlation between species diversity and the abundance of antibodypositive O. longicaudatus (Piudo et al. 2011) . We recommend reporting data on both prevalence and density of infection (in vectors and/or vertebrate hosts) so that public health agencies can interpret how biodiversity affects these key components of disease risk. Parallel issues arise when attempting to interpret measures of biodiversity: is species richness, evenness or some index the best indicator of community biodiversity?

We only included studies that explicitly measured biodiversity, because proxies may fail to accurately represent local community ecology (Wood & Lafferty 2012) . When species richness was explicitly measured, there was little evidence of a significant relationship with Lyme disease risk (Prusinski et al. 2006; LoGiudice et al. 2008) . Studies that do use substitute measures (e.g. forest fragmentation) provide conflicting results: a negative correlation between forest fragmentation and Lyme disease risk in New York & Connecticut (Allan et al. 2003; Brownstein et al. 2005) ; no relationship across New York, Connecticut and New Jersey (LoGiudice et al. 2008); and lower human incidence of Lyme disease in fragmented contexts in Connecticut (Brownstein et al. 2005) .

Rather than biodiversity, it is more likely that the role of individual host species and their interactions with other hosts, vectors, and the pathogen that are more influential in determining local disease risk (Kilpatrick et al. 2006; LoGiudice et al. 2008; Salkeld & Lane 2010; Hamer et al. 2011) . For example, the reservoir competence and abundance of particular bird species and the mosquito vector's feeding preferences may be better predictors of West Nile virus activity than avian species richness (Kilpatrick et al. 2006; Loss et al. 2009; Hamer et al. 2011) . In Utah's Great Basin Desert, pinyon mice (Peromyscus truei) appear to reduce hantavirus antibody prevalence, whereas kangaroo rats (Dipodomys ordii) seem to increase hantavirus antibody prevalence (Clay et al. 2009) , and in Montana, estimated Sin Nombre antibody prevalence in deer mice was reduced in the presence of voles (Microtus spp.), although vole abundance was not important (Carver et al. 2011) .

At a larger spatial scale, some studies have demonstrated negative correlations between measures of diversity and disease, for example, human WNV incidence at the county level negatively correlated with data from breeding bird atlases or surveys (Ezenwa et al. 2006; Swaddle & Calos 2008; Allan et al. 2009 ). In contrast, mammal diversity at a global scale has been linked to increased likelihood of emerging zoonotic pathogens (Jones et al. 2008) . We believe that these studies should be interpreted with caution given that vertebrate diversity, incidence of zoonoses, vector feeding ecology and infection rates are often recorded at different times or spatial scales and rely on disparate sources of data. That is, disease transmission can operate at fine spatial scales and data at coarse county or region levels may not be indicative of local pathogen transmission patterns (Loss et al. 2009; Hamer et al. 2011) . When data from larger aggregates are used to infer the properties of the elements of those aggregates, epidemiologists warn of an 'ecological fallacy,' and even though ecologists may bristle at the term, the effect is real and avoidable. We have attempted to avoid this fallacy by not including county-level studies.

We did not include studies that examine non-human pathogens in our meta-analysis because our interest was in the possible relationship between wildlife biodiversity and human health. However, we presume that impacts of biodiversity on non-human diseases will also depend on local ecology and the particular host-pathogen relationships (e.g. Hudson et al. 2006; Salkeld et al. 2008; Chasar et al. 2009; Wood et al. 2010) . For example, a recent meta-analysis, found that relationships between primate parasite prevalence and mammal species richness were idiosyncratic (Young et al. 2013 ).

If we are to gain an understanding of causal mechanisms generating diversity-disease relationships, investigations into the relationship between biodiversity and zoonotic disease risk must collect data on biodiversity that is spatially and temporally related to the relevant measures of disease risk. For example, WNV infection rates in mosquitoes should be related to the bird community that fed the mosquito population. Investigations must acknowledge variation in vector feeding behaviour (host species preferences), vector and host transmission biology, and the influences of abiotic factors (e.g. temperature, rainfall) and habitat types upon disease dynamics (Randolph & Dobson 2012) . Measures of biodiversity should incorporate additional species that may interact with reservoir species, for example, carnivores that might influence rodent host population biology and thereby influence hantavirus or B. burgdorferi transmission dynamics (Ostfeld & Holt 2004) . A promising approach to understanding interactions between disease ecology and epidemiology is to focus on tractable manipulations such as removal of particular host or vector species (e.g. Tsao et al. 2004; Perkins et al. 2006; Suz an et al. 2009 ). Also, analyses must entertain and control for alternate hypotheses that affect both biodiversity and disease risk, such as rainfall gradients, urbanisation, vector abundance, human behaviour, latitude. Such study designs, incorporating ecologists, epidemiologists, medical entomologists and public health agencies may facilitate an understanding of exactly what metrics are required to understand the local disease risk, and the mechanisms that allow zoonotic disease to spill over into human cases.

Public health initiatives intending to ameliorate disease risk must rely on a comprehensive, empirically based understanding of local disease ecology and epidemiology. Our analyses suggest that the links between biodiversity and disease prevalence are variable and dependent on the disease system, local ecology and probably human social context. Broadly advocating for the preservation of biodiversity and natural ecosystems to reduce disease risk is an oversimplification of disease ecology and epidemiology. If the disease dynamics are not well understood, conservation of endemic biodiversity could, in fact, increase human disease risk (e.g. if density of reservoirs or vectors increases), and may simultaneously remove economic and logistical resources from more effective control measures (Randolph & Dobson 2012) . Certainly, the links between measures of disease risk (e.g. infection rates in reservoir hosts or vectors) and actual human incidence (rates of infection in the local human populations) need to be better established. Furthermore, how and where humans are exposed has important consequences for the biodiversity-disease buffer hypothesis, that is are the scales at which biodiversity and disease risk are investigated actually relevant to human exposure to disease?

It is possible that the hope for a win-win outcomesimultaneous protection of biodiversity and human healthhas led to premature acceptance that the dilution effect is a general ecosystem service associated with biodiversity (Randolph & Dobson 2012) . However, our meta-analysis suggests that the dilution effect is neither general nor strong. The ecology of infectious disease is often too complicated to expect that there will be simple relationships between disease risk and biodiversity. A focus on the local ecology and interactions of important hosts and vectors is more likely to reveal insights into how to reduce disease risk and humans. 

Effect of forest fragmentation on Lyme disease risk

Ecological correlates of risk and incidence of West Nile virus in the United States

Conspicuous impacts of inconspicuous hosts on the Lyme disease epidemic

A null model of community disassembly effects on vector-borne disease risk

Forest fragmentation predicts local scale heterogeneity of Lyme disease risk

A temporal dilution effect: hantavirus infection in deer mice and the intermittent presence of voles in Montana

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Prevalence and diversity patterns of avian blood parasites in degraded African rainforest habitats

Sin Nombre virus and rodent species diversity: a test of the dilution and amplification hypothesis

The Handbook of Research Synthesis and Meta-Analysis

Increased host species diversity and decreased prevalence of Sin Nombre virus

A nonparametric "trim and fill" method of accounting for publication bias in meta-analysis

Trim and fill: a simple funnel-plot-based method of testing and adjusting for publication bias in meta-analysis

Avian diversity and West Nile virus: testing associations between biodiversity and infectious disease risk

Emergence of tick-borne granulocytic Anaplasmosis associated with habitat type and forest change in northern California

Fine scale variation in vector host use and force of infection drive localized patterns of West Nile virus transmission

Statistical Methods for Meta-Analysis

Is a healthy ecosystem one that is rich in parasites?

Global trends in emerging infectious diseases

Effects of species diversity on disease risk

Impacts of biodiversity on the emergence and transmission of infectious diseases

Host heterogeneity dominates West Nile virus transmission

Experimental infection of North American birds with the New York 1999 strain of West Nile virus

The ecology of infectious disease: effects of host diversity and community composition on Lyme disease risk

Impact of host community composition on Lyme disease risk

Avian host community structure and prevalence of West Nile virus in

Regulation or rodent-borne viruses in the natural host: implications for human disease

Ectoparasite diversity and exposure to vector-borne disease agents in wild rodents in central coastal California

Biodiversity and Lyme disease: dilution or amplification

Biogeographic and ecological regulation of disease: prevalence of Sin Nombre Virus in island mice is related to island area, precipitation, and predator richness

Are predators good for your health? Evaluating evidence for top-down regulation of zoonotic disease reservoirs

Localized deer absence leads to tick amplification

Rodent community structure and Andes Virus infection in sylvan and peridomestic habitats in northwestern Patagonia. Argentina. Vector-borne Zoon

Biodiversity loss and the new global disease ecology

Habitat structure associated with Borrelia burgdorferi prevalence in small mammals in New York state

Pangloss revisited: a critique of the dilution effect and the biodiversity-buffers-disease paradigm

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Community ecology and disease risk: lizards, squirrels and the Lyme disease spirochete in California

Parasite loads are higher in the tropics: temperate to tropical variation in a single host-parasite system

Plague outbreaks in prairie dog populations explained by percolation thresholds of alternate host abundance

Confidence intervals for the overall effect size in random-effects meta-analysis

Biodiversity and the dilution effect in disease ecology

A simple confidence interval for meta-analysis

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Experimental evidence for reduced rodent diversity causing increased hantavirus prevalence

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Does habitat disturbance increase infectious disease risk for primates?

We thank many colleagues for helpful discussion on these issues, and for sharing information and data: B