key: cord-0765795-9t15p537
authors: Monge, Cecilia; Xie, Changqing; Steinberg, Seth M; Greten, Tim F
title: Clinical Indicators for Long-Term Survival with Immune Checkpoint Therapy in Advanced Hepatocellular Carcinoma
date: 2021-05-31
journal: J Hepatocell Carcinoma
DOI: 10.2147/jhc.s311496
sha: f496942a746b113035170380b10fb4d10729f889
doc_id: 765795
cord_uid: 9t15p537

INTRODUCTION: Patients with advanced hepatocellular carcinoma have a dismal prognosis; only a subset of patients with advanced HCC will benefit from treatment with immunotherapy. We searched for clinical characteristics predicting exceptional long-term survival in HCC patients treated with immune checkpoint inhibitors. METHODS: We compared clinical characteristics of 59 patients with advanced hepatocellular carcinoma treated with immunotherapy with and without locoregional therapy between 2013–2019. We compared patients who lived less than 12 months with patients who lived more than 3 years. Traits of short-term (31 patients) and long-term (5 patients) survivors were compared. Patients who died between 12 months and 3 years of starting treatment on protocol were not included in the analysis. RESULTS: Two out of five patients (40%) in the long-term survival group had a partial response (PR) or a complete response (CR) per the modified Response Evaluation Criteria in Solid Tumors (mRECIST), while, of the 31 patients in the short-term survival group, only 2 (6.5%) had a CR or PR. Two of the 5 patients with a long-term survival had immune-related adverse events grade 3 or 4 (IrAEs-3/4). None of the patients in the short-term survival group had IrAEs-3/4. The patients, who presented with IrAEs-3/4, which included colitis and adrenal insufficiency, continued to have a response off treatment. The median overall survival (OS) was 11.8 months (95% CI: 7.8–15.4 months), with a 12-month OS of 46.6% (95% CI: 33.4–58.8%) and a 3-year OS of 12.5% (95% CI: 5.0–23.7%). CONCLUSION: We found a possible association between immune-related adverse events grade 3 and 4 and long-term survival in patients with advanced HCC. The cases in our analysis represent extraordinary defiance of the usual predicted dismal course of advanced HCC.

Patients with advanced hepatocellular carcinoma have a dismal prognosis. 1 In the last three and a half years, four immune checkpoint inhibitors have received FDA approval for the treatment of advanced HCC in the first-or second-line setting, transforming current standards of care. [2] [3] [4] Only a subset of patients with advanced HCC will benefit from treatment with immunotherapy, which highlights a pressing need to identify predictive characteristics of a good response to treatment. We searched for clinical characteristics predicting exceptional long-term survival in HCC patients treated with immune checkpoint inhibitors in clinical trials.

From July 2013 to November 2019, we enrolled 59 patients with advanced HCC on two clinical trials testing the combination of tremelimumab 5 or tremelimumab plus durvalumab and locoregional therapy (NCT01853618, NCT03937830). We compared patients who lived less than 12 months (31 patients) with patients who lived more than 3 years (5 patients). Patients who died between 12 months and 3 years of starting treatment on protocol were not included in the analysis. Traits of short-and longterm survivors were compared using Fisher's exact test for dichotomous traits. A Cochran-Armitage test was used to identify the alpha fetoprotein (AFP) trend and an exact Wilcoxon rank sum test was used to compare ages and prior duration of treatment with sorafenib.

Comparison of the clinical characteristics of the two groups included age, sex, duration of sorafenib treatment prior to study, AFP value, response to treatment per RECIST criteria, tumor burden and immune-related adverse events grade 3 or 4 (IrAEs-3/4) ( Table 1) . Alphafeto-protein values were allocated to three arbitrary categories: 1 to 20 ng/mL (1), 21 to 1000 ng/mL (2) and 1001 ng/mL or higher (3). High tumor burden was arbitrarily defined as a tumor with a diameter of >5 cm at start of treatment in the study. Five patients had impressive survivals, ranging from 40 to 74 months; 31 patients died in the first year. The median age was 64 years in the long-term survival patients and 61.8 years in the short-term survival group; most patients had an Eastern Cooperative Oncology Group (ECOG) of 1. As expected for HCC from an epidemiological perspective, most patients were male; all patients in the long-term survival group and approximately 65% of patients in the short-term survival group were male. Hepatitis C-related cirrhosis was the most common underlying liver disease in both groups, followed by hepatitis B. Most patients in both groups had a Child-Pugh score consistent with class A. Patients in the long-term survival group received a mean of 6.2 months of treatment with sorafenib prior to enrollment compared to 4.2 months of treatment in the short-term survival group. Approximately 40% of patients in both groups had AFP values in category 3. A high tumor burden was present in 4 of 5 patients (80%) with long-term survival and in 22 of 29 patients (75.9%) with short-term survival. In the group of patients with long-term survival, 2 out of 5 (40%) had a partial response (PR) or a complete response (CR) per the modified Response Evaluation Criteria in Solid Tumors (mRECIST), 6 while within the 31 patients in the short-term survival group only 2 (6.5%) of 31 patients had a CR or PR. Two of the 5 patients with a long-term survival had IrAEs-3/4. None of the patients in the shortterm survival group had IrAEs-3/4. The 2 patients who presented with IrAEs-3/4, which included colitis and adrenal insufficiency, continued to have a response off treatment. The median overall survival (OS) was 11.8 months (95% CI: 7.8-15.4 months), with a 12-month OS of 46.6% (95% CI: 33.4-58.8%) and a 3-year OS of 12.5% (95% CI: 5.0-23.7%).

Two of the five patients with long-term survival died of non-HCC-related causes, including myocardial infarction and cerebrovascular accident, and two patients are still alive. The five long-term survival patients all received treatment with locoregional therapy prior to enrolling on the study, two underwent a partial hepatectomy and three were treated with sorafenib (treatment duration ranged from 2 to 6 months). Three patients were considered Barcelona Clinic Liver Cancer (BCLC) B with HCC limited to the liver, one patient had metastatic disease to soft tissue, and one had metastatic disease to both soft tissue and bone. Three patients had underlying hepatitis C as the cause of cirrhosis. One patient did not have any known underlying liver disease. The range of time from diagnosis to enrollment on study was between 3 and 58 months. The overall survival in the five long-term survival patients ranged from 40 to 74 months. Three patients received locoregional treatment in combination with immunotherapy while on study and two patients received combination immunotherapy with durvalumab and tremelimumab. As a group, they received between 6 and 40 cycles of treatment on study. Two patients in the long-term survival group came off study because of PD, one patient came off because of colitis which was classified as an IrAE, one patient discontinued treatment after 38 cycles due to personal preference and one patient continues on study ( Table 2 ).

We provide a unique insight into long-term survival of patients with advanced HCC treated with immune checkpoint inhibition (ICI) by comprehensively analyzing clinical characteristics that may identify the subset in which treatment results in prolonged disease control. Although 20% of patients with advanced HCC are expected to have a response 

to ICI, 7 our data shows the existence of a subgroup within the responders that will have an overall survival much longer than described in the literature. Patient age, sex and duration of prior treatment with sorafenib were not associated with length of survival. Higher AFP level is generally considered to be a biomarker associated with a decreased overall survival in HCC; 8, 9 the AFP level in our patients did not show a correlation with survival. Our analyses showed that patients with a higher burden of disease did not necessarily die earlier, as may be expected. Based on a limited number of patients, we propose that a response to treatment is more likely to be associated with a longer survival compared to stable disease or progressive disease, although this did not reach statistical significance. There was an association between IrAEs-3/4 and a long-term overall survival; both patients who presented with IrAEs-3/4 had a long overall survival. This result is clearly hypothesis-generating and would need to be confirmed in an independent subsequent study before suggesting this association is truly meaningful. Interestingly, the appearance of IrAEs upon treatment with anti-PD-1 and anti-PD-L1 antibodies, 10,11 such as durvalumab, has also correlated with improved outcomes such as longer OS, higher response rates and longer progression-free survival in various cancers, 12, 13 including non-small-cell lung cancer [14] [15] [16] [17] and melanoma. 18 IrAEs are considered a potential marker for response to ICI, with the caveat that they present themselves upon treatment. Patients who respond to ICI may be more likely to have what is considered to be a responsive immune system and a higher incidence of autoimmunity when exposed to immunotherapy as a result of activated T cells targeting antigens present on both tumor and inflamed organ resulting in IrAEs as well as clinical response to treatment. 19, 20 However, the exact mechanism has not been described. Mixed results are reported in the literature on the association between anti-CTLA4 antibodies such as tremelimumab, IrAEs and treatment efficacy, although there seems to be an overall association between IrAEs and an improved survival. [21] [22] [23] [24] The predictive value of IrAEs in patients treated with ICI continues to be studied.

The cases in our analysis represent extraordinary defiance of the usual predicted dismal course of advanced HCC. Our analysis further highlights the current need to identify markers for HCC patients that may derive benefit in overall survival with immunotherapy treatment.

The 

Informed consent was obtained from all subjects involved in the study.

All authors made substantial contributions to conception and design, acquisition of data, or analysis and interpretation of data; took part in drafting the article or revising it critically for important intellectual content; agreed to submit to the current journal; gave final approval of the version to be published; and agree to be accountable for all aspects of the work.

This research was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government.

The authors declare no conflicts of interest.

Hepatocellular carcinoma

Atezolizumab plus bevacizumab in unresectable hepatocellular carcinoma

Efficacy and safety of nivolumab plus ipilimumab in patients with advanced hepatocellular carcinoma previously treated with sorafenib: the checkmate 040 randomized clinical trial

Pembrolizumab in patients with advanced hepatocellular carcinoma previously treated with sorafenib (KEYNOTE-224): a non-randomised, open-label Phase 2 trial

Tremelimumab in combination with ablation in patients with advanced hepatocellular carcinoma

Objective response by mRECIST as a predictor and potential surrogate endpoint of overall survival in advanced HCC

Nivolumab in patients with advanced hepatocellular carcinoma (CheckMate 040): an open-label, non-comparative, Phase 1/2 dose escalation and expansion trial

Ramucirumab after sorafenib in patients with advanced hepatocellular carcinoma and increased alpha-fetoprotein concentrations (REACH-2): a randomised, double-blind, placebo-controlled, Phase 3 trial

Clinical characteristics and prognosis of hepatocellular carcinoma: analysis based on serum alpha-fetoprotein levels

Lenvatinib versus sorafenib in firstline treatment of patients with unresectable hepatocellular carcinoma: a randomized phase 3 non-inferiority trial

Analysis of the association between adverse events and outcome in patients receiving a programmed death protein 1 or programmed death ligand 1 antibody

Immunerelated adverse events predict the therapeutic efficacy of anti-PD-1 antibodies in cancer patients

Are immune-related adverse events associated with the efficacy of immune checkpoint inhibitors in patients with cancer? A systematic review and meta-analysis

Immune-related adverse events correlate with clinical outcomes in NSCLC patients treated with nivolumab: the Italian NSCLC expanded access program

Association of immune-related adverse events with clinical benefit in patients with advanced non-small-cell lung cancer treated with nivolumab

Association between immune-related adverse events and efficacy of immune checkpoint inhibitors in non-small-cell lung cancer

Correlation between immune-related adverse events and efficacy in non-small cell lung cancer treated with nivolumab

Association between immune-related adverse events and clinical efficacy in patients with melanoma treated with nivolumab: a multicenter retrospective study

Immune-related adverse events and anti-tumor efficacy of immune checkpoint inhibitors

Clinical features, predictive correlates, and pathophysiology of immune-related adverse events in immune checkpoint inhibitor treatments in cancer: a short review

Physiopathological mechanisms of immune-related adverse events induced by anti-CTLA-4, anti-PD-1 and anti-PD-L1 antibodies in cancer treatment

Prognostic factors related to clinical response in patients with metastatic melanoma treated by CTL-associated antigen-4 blockade

Autoimmunity correlates with tumor regression in patients with metastatic melanoma treated with anti-cytotoxic T-lymphocyte antigen-4

Phase I/II study of ipilimumab for patients with metastatic melanoma