key: cord-0765711-as9usx4t
authors: Catella, Cristiana; Camero, Michele; Lucente, Maria Stella; Fracchiolla, Giuseppe; Sblano, Sabina; Tempesta, Maria; Martella, Vito; Buonavoglia, Canio; Lanave, Gianvito
title: Virucidal and antiviral effects of Thymus vulgaris essential oil on feline coronavirus
date: 2021-04-22
journal: Res Vet Sci
DOI: 10.1016/j.rvsc.2021.04.024
sha: f3e015d6450085f5363ec72d69820180d824c77b
doc_id: 765711
cord_uid: as9usx4t

Feline infectious peritonitis (FIP) is a fatal systemic disease of felids caused by a Coronavirus (CoV) (FIPV). In spite of its clinical relevance and impact on feline health, currently the therapeutic possibilities for treatment of FIP in cats are limited. The emergence of the pandemic Severe Respiratory Syndrome (SARS) coronavirus (CoV) type 2 (SARS-CoV-2), etiological agent of the 2019 Coronavirus Disease (COVID-19), able to infect a broad spectrum of animal species including cats, triggered the interest for the development of novel molecules with antiviral activity for treatment of CoV infections in humans and animals. Essential oils (EOs) have raised significant attention for their antiviral properties integrating and, in some cases, replacing conventional drugs. Thymus vulgaris EO (TEO) has been previously shown to be effective against several RNA viruses including CoVs. In the present study the antiviral efficacy of TEO against FIPV was evaluated in vitro. TEO at 27 μg/ml was able to inhibit virus replication with a significant reduction of 2 log10 TCID(50)/50 μl. Moreover, virucidal activity was tested using TEO at 27 and 270 μg/ml, over the cytotoxic threshold, determining a reduction of viral titre as high as 3.25 log10 TCID(50)/50 μl up to 1 h of time contact. These results open several perspectives in terms of future applications and therapeutic possibilities for coronaviruses considering that FIPV infection in cats could be a potential model for the study of antivirals against CoVs.

NAs/10μl, respectively was observed (Fig. 1B) .

FCoV-II was assessed (Fig. 2) 

type 1 and type 2 in vitro

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Efficacy and safety of the nucleoside analog GS-441524 for treatment of cats with naturally occurring feline infectious peritonitis

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Evaluating the Efficacy of Achillea millefolium and Thymus vulgaris Extracts Against Newcastle Disease Virus in Ovo

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The authors declare that they have no conflict of interest.