key: cord-0765516-f8606yr6 authors: del Nonno, Franca; Frustaci, Andrea; Verardo, Romina; Chimenti, Cristina; Nicastri, Emanuele; Antinori, Andrea; Petrosillo, Nicola; Lalle, Eleonora; Agrati, Chiara; Ippolito, Giuseppe title: Virus-Negative Myopericarditis in Human Coronavirus Infection: Report From an Autopsy Series date: 2020-11-12 journal: Circulation. Heart failure DOI: 10.1161/circheartfailure.120.007636 sha: c8650616ee85006e9947fb605270ebb01d171268 doc_id: 765516 cord_uid: f8606yr6 Supplemental Digital Content is available in the text. T he cause of death in patients with coronavirus disease 2019 (COVID-19) infection has not yet been completely clarified. In addition to lung failure, cardiac inflammation has also been suggested to occur. However, its source from a direct or indirect COVID-19 involvement, type of mechanism, that is, cytopathic versus immune-mediated, and compartments involved (vessels, nerves, and conduction tissue) are still undefined. We report cardiac autopsy findings in 9 patients ( Table) . At macroscopic examination, the heart was always increased in weight ( Figure 1A ), pale and flabby (Figure 1B) , and in 6, a pericardial effusion ( Figure 1A ) was also observed. No relevant (>70%) stenosis of epicardial coronary arteries was documented. At histology, lymphomononuclear infiltrates (CD68, CD45 Ro positive) were observed in the myocardium with focal necrosis of adjacent myocytes ( Figure 1C and 1D), in all patients and in the pericardium ( Figure 1E and 1F) and in intramural vessels with necrosis of vessels' wall ( Figure 1G and 1H) in the 6 subjects who died because of heart failure. Inflammatory infiltrates were mostly registered in the adventitia and outer vessel layer. Vasculitis of pulmonary vessels (Figures I and II in the Data Supplement) was commonly observed in our cohort. In the 3 patients with ventricular arrhythmias, who suffered sudden death, subepicardial ganglia were also involved by inflammation with degradation of neuron fibers ( Figure 1I ). In the last cohort, several sections including peripheral segments of His-Purkinje system presented lymphocytic infiltration and necrosis of conduction tissue ( Figure 1J ). The extensive involvement of intramural coronary vessels in these patients suggests to have been, in addition to cardiomyocyte necrosis, a major determinant of heart failure and cardiogenic shock. Inflammation of cardiac conduction tissue and of subepicardial ganglia documented in 3 patients gives reason for the occurrence of severe electrical instability and sudden death. Real-time polymerase chain reaction has been obtained from several frozen myocardial samples for the most common cardiotropic viral genomes, including adenovirus, cytomegalovirus, parvovirus B19, Epstein-Barr virus, human herpes virus 6, and herpes simplex virus 1 and 2, enterovirus, influenza virus A and B, hepatitis C virus, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Molecular studies failed to find viral genomes, including SARS-CoV-2, in the myocardium of our cohort. Polymerase chain reaction for SARS-CoV-2 was always positive in the lung tissue. These findings strongly suggest myocardial inflammation in COVID-19 infection contributes significantly to patients' death. To this regard, all cardiac compartments may be involved including pericardium, myocardium, coronary vessels, conduction tissue, and neuron ganglia. Our autopsy series demonstrated myocardial inflammation in the absence of viral genomes most likely related to an abnormal immunologic response. 1 Its severity is probably linked to the specificity of human leukocyte antigen (HLA) system and explains the different clinical response to SARS-CoV-2 infection: indeed, some patients have a mild or a moderate form of myocarditis and recover spontaneously, whereas others may have a severe manifestation and die. A recent report demonstrates that HLA variation affects the cellular immune response to peptides from human-infecting coronaviruses with HLA-B*46:01 causing increased and HLA-B*15:01 reduced vulnerability to viral infection. 2 Lack of detection of SARS-CoV-2 in the myocardium is at variance with another previously published case report 3 showing ultrastructural evidence of viral particles in cardiac macrophages. This probably represents a hyperacute and highly viremic phase of the COVID-19 disease, with a direct viral damage, which is likely followed after some days, as in our cases, by an immune-mediated insult. Differently from recent observation of endothelitis 4,5 caused by SARS-CoV-2, coronary vasculitis in our study proceeded from adventitia to internal vessel layers ( Figure 1C and 1D) suggesting the immunologic damage coming from vasa vasorum. We can speculate that immunocytes and circulating immunocomplexes might participate in vessel inflammation as recently suggested. 5 Pathological implication in this instance is the loss of pericytes leading to additional impairment of angiogenesis. 6 Clinical implications from these observations are that these patients may respond to immune-modulation therapy, including steroids, inhibitors of interleukin-1β, and inhibitors of receptors for interleukin-6, in addition to an antiviral therapy. The presence of extensive necrotizing vasculitis of intramural coronary vessels solicits the administration of high-dosage immune-modulating agents. The levels of fibrinogen, CRP, d-dimer, lactate dehydrogenase (LDH), troponin I, and lymphocytes were evaluated by diagnostic tools in 9 pts with coronavirus infection. CRP indicates C-reactive protein; and na, not available. Department of Clinical, Internal, Anesthesiologist and Cardiovascular Sciences Acute myocarditis presenting as a reverse Tako-Tsubo syndrome in a patient with SARS-CoV-2 respiratory infection Human leukocyte antigen susceptibility map for severe acute respiratory syndrome coronavirus 2 Myocardial localization of coronavirus in COVID-19 cardiogenic shock Endothelial cell infection and endotheliitis in COVID-19 Complement associated microvascular injury and thrombosis in the pathogenesis of severe Covid-19 infection: a report of five case The role of pericytes in blood-vessel formation and maintenance Figure 1. Cardiac postmortem findings in 9 patients affected by coronavirus disease 2019 (COVID-19) infection Overweight heart (450 g) in a patient with coronavirus infection. Plaque-like fibrous whitish thickened pericardium denoting pericarditis. B, Grossly, the heart was dilated, and the ventricular myocardium was flabby and pale with thickened walls D, Immunohistochemistry for cluster differentiation68 showing positive macrophages in the inflamed myocardium. E and F, Lymphocytic pericarditis (hematoxlin and eosin, 200×). G, Necrotizing vasculitis of an intramural coronary artery (hematoxlin and eosin, 200×). H, Immunohistochemistry for Cluster differentiation45Ro suggesting lymphocytic infiltration and necrosis of an intramural coronary artery (200×). I, Lymphocytic infiltration of a subepicardial ganglion (cluster differentiation45Ro, 400×). J, Severe infiltration with damage of a section of conduction tissue None.