key: cord-0765482-dtv7to3l authors: Liu, Yen-Chin; Kuo, Rei-Lin; Shih, Shin-Ru title: COVID-19: the First Documented Coronavirus Pandemic in History date: 2020-05-05 journal: Biomed J DOI: 10.1016/j.bj.2020.04.007 sha: c8c7e763297bc485ab8b6130c092225d339d0840 doc_id: 765482 cord_uid: dtv7to3l Abstract The novel human coronavirus disease COVID-19 has become the fifth documented pandemic since the 1918 flu pandemic. COVID-19 was first reported in Wuhan, China, and subsequently spread worldwide. The coronavirus was officially named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by the International Committee on Taxonomy of Viruses based on phylogenetic analysis. SARS-CoV-2 is believed to be a spillover of an animal coronavirus and later adapted the ability of human-to-human transmission. Because the virus is highly contagious, it rapidly spreads and continuously evolves in the human population. In this review article, we discuss the basic properties, potential origin, and evolution of the novel human coronavirus. These factors may be critical for studies of pathogenicity, antiviral designs, and vaccine development against the virus. agent is a novel coronavirus. Therefore, this virus is the seventh member of the coronavirus family to infect humans [3] . The World Health Organization (WHO) temporarily termed the new virus 2019 novel coronavirus (2019-nCoV) on 12 January 2020 and then officially named this infectious disease coronavirus disease 2019 (COVID-19) on 12 February 2020. Later, the International Committee on Taxonomy of Viruses (ICTV) officially designated the virus as SARS-CoV-2 based on phylogeny, taxonomy and established practice [4] . Subsequently, human-to-human transmission of COVID-19 occurring within Hong Kong has been shown in clinical data [5] . Since initially emerged in China, the virus has evolved for four months and rapidly spread to other countries worldwide as a global threat. On 11 March 2020, the WHO finally made the assessment that COVID-19 can be characterized as a pandemic, following 1918 Spanish flu (H1N1), 1957 Asian flu (H2N2), 1968 Hong Kong flu (H3N2), and 2009 Pandemic flu (H1N1), which caused an estimated 50 million, 1.5 million, 1 million, and 300,000 human deaths, respectively [6] [7] [8] [9] [Fig. 1 ]. SARS-CoV-2 is an enveloped and spherical particle approximately 120 nm in diameter containing a positive-sense single-stranded RNA genome. It belongs to the subfamily Coronavirinae, family Coronavirdiae, and order Nidovirales. The RNA genome of SARS-CoV-2 contains a 5' methyl-guanosine cap, poly (A)-tail, and 29,903 nucleotides according to WH-Human 1 coronavirus (WHCV) [3, 10] . It is classified as a beta-coronavirus (βCoV) [ [11] [12] [13] [14] . It has been shown that bats and rodents are the gene sources of most αCoVs and βCoVs, however, avian species are the gene sources of most δCoVs and γCoVs by evolutionary analyses. The human coronavirus (HCoV) strains HCoV-NL63, HCoV-229E, HCoV-HKU1, and HCoV-OC43 usually cause mild, self-limiting upper respiratory tract infections, such as the common cold [15, 16] . However, SARS-CoV, MERS-CoV, and SARS-CoV-2 can cause severe acute respiratory syndrome and result in life-threatening disease [17] [18] [19] [ Table 1 ]. SARS-CoV-2 transcribes nine subgenomic RNAs, and its genome comprises a 5' untranslated region including a 5' leader sequence; an open reading frame (ORF) 1a/ab encoding nonstructural proteins (nsp) for replication; four structural proteins including spike (S), envelope (E), membrane (M) and nucleocapsid (N); several accessory proteins such as ORF 3a, 6, 7a/b, and 8; and a 3' untranslated region. The replicase polyprotein pp1a/ab encodes and is proteolytically cleaved into 16 putative nsps, including nsp3 (papain-like protease), nsp5 (3C-like protease), nsp12 (RNA-dependent RNA polymerase [RdRp]), nsp13 (helicase), and other nsps [10, 13, 20] . The spike glycoprotein of SARS-CoV-2 binds to angiotensin-converting enzyme 2 (ACE2) in human and Chinese horseshoe bats, civet for cell entry, that is also dependent on S protein priming by the serine protease TMPRSS2. A similar panel of mammalian cell lines can be infected with SARS-CoV-2-S and SARS-CoV-S [21] [22] [23] [24] . The spike protein could be cleaved by host proteases into the S1 and S2 subunits, which are responsible for receptor recognition and membrane fusion, respectively. S1 also can be divided into an N-terminal domain (NTD) and a C-terminal domain (CTD). The S1 CTD of SARS-CoV-2, but not the NTD, showed strong affinity for human ACE2 (hACE2). The receptor-binding domain (RBD) within SARS-CoV-2 CTD is the key region that interacts with the hACE2 receptor with higher affinity than the RBD of SARS-CoV by 10-to 20-fold with kinetic quantification [23, 25] . All human coronaviruses have animal origins, namely, natural hosts. Bats may be the natural hosts of HCoV-229E, SARS-CoV, HCoV-NL63, and MERS-CoV. Furthermore, HCoV-OC43 and HKU1 probably originated from rodents [26] [27] [28] . Bats are undoubtedly important and the major natural reservoirs of alpha-coronaviruses and beta-coronaviruses [29] . Domestic animals can suffer from disease as intermediate hosts that cause virus transmission from natural hosts to humans; for example, SARS-CoV and MERS-CoV crossed the species barriers into masked palm civets and camels, respectively [30, 31] [ Table 1 ]. SARS-CoV-2 sequenced at the early stage of the COVID-19 outbreak only shares 79.6% sequence identity with SARS-CoV through early full-length genomic comparisons. However, it is highly identical (96.2%) at the whole-genome level to Bat-CoV RaTG13, which was previously detected in Rhinolophus affinis from Yunnan Province, over 1,500 km from Wuhan [21] . Bats are likely reservoir hosts for SARS-CoV-2; however, whether Bat-CoV humans because of the similarity of the pangolin coronavirus to SARS-CoV-2 [32, 33] . The additional analysis of phylogenetic networks can be effective and useful to trace undocumented COVID-19 infection sources [34] . In addition to the zoonotic origins of SARS-CoV-2 by natural evolution, there are still some disputes about the origin of the virus because its spike protein seems to perfectly interact with the human receptor in contributing to human-to-human transmission after evolution in a short period. Nevertheless, more direct evidence is required to clarify the arguments. Replication of RNA viruses could generate mutations due to the low proofreading ability of their RdRP. The genome variations generated by viral RdRP could be beneficial for an emerging virus to adapt to new hosts. However, previous studies have shown that the mutation rates could vary in RNA viruses [35] . The synonymous substitution rate for coronaviruses might be approximately 1 × 10 -3 /synonymous site/year, which is lower than some other RNA viruses. The mutation rate during coronavirus replication could be partially controlled by the viral exoribonuclease nsp14 [36, 37] . Nevertheless, SARS-CoV-2 has been continuously evolving to different groups worldwide during the pandemic. According to the information of nCoV-19 (SARS-CoV-2) sequences submitted to the GISAID database in January 2020, the virus was first collected in late December 2019 from Wuhan, China. However, those viral sequences varied from the latest submitted sequence collected in early April 2020 from North America. Since the viral sequences continuously change, the construction of a phylogenetic network is crucial to investigate the adaption of the virus in different human populations and environments. Although the virus keeps evolving within humans who could also be susceptible to other human coronaviruses, recombination between SARS-CoV-2 and old human coronaviruses, such as HCoV-229E, OC43, NL63, and HKU1, has not been found. Nevertheless, a recent study revealed that three genetic types, A, B, and C, of the virus have been circulating globally [34] . The study demonstrated that the genotypes could also correlate to the geographic locations. This indicates that the evolution of SARS-CoV-2 may be affected by replication environments, such as genetic and immunological restrictions in different human populations. With evolutionary pressure, the selection of SARS-CoV-2 mutations will be ongoing. The investigation of the geographic patterns of SARS-CoV-2 variations will provide information on vaccine development for different populations. Human coronaviruses usually cause mild upper respiratory diseases. However, in the past two decades, two coronaviruses transmitted from animals, SARS-CoV and MERS-CoV, have caused severe pneumonia and death in humans. In addition, since late December 2019, the COVID-19 pandemic has spread globally and consequently resulted in at least 190,549 deaths worldwide as of April 24, 2020 [38] . Due to the high sequence homology with a coronavirus isolated from bats, SARS-CoV-2 is considered a zoonotic origin coronavirus. Undoubtedly, SARS-CoV-2 has become the fifth human coronavirus, and it is possible that this virus will continuously circulate in the human population in the future. Because specific antiviral treatments and vaccines are still under development, testing, quarantine, and social distancing are encouraged to prevent virus spread. 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