key: cord-0762688-5ecg1bop authors: Palumbo, Diego; Zangrillo, Alberto; Belletti, Alessandro; Guazzarotti, Giorgia; Calvi, Maria Rosa; Guzzo, Francesca; Pennella, Renato; Monti, Giacomo; Gritti, Chiara; Marmiere, Marilena; Rocchi, Margherita; Colombo, Sergio; Valsecchi, Davide; Scandroglio, Anna Mara; Dagna, Lorenzo; Rovere-Querini, Patrizia; Tresoldi, Moreno; Landoni, Giovanni; De Cobelli, Francesco title: A radiological predictor for pneumomediastinum/pneumothorax in COVID-19 ARDS patients date: 2021-08-12 journal: J Crit Care DOI: 10.1016/j.jcrc.2021.07.022 sha: b24fa7becb63112adcaf19a9710ebd882f5ce978 doc_id: 762688 cord_uid: 5ecg1bop PURPOSE: To determine whether Macklin effect (a linear collection of air contiguous to the bronchovascular sheath) on baseline CT imaging is an accurate predictor for subsequent pneumomediastinum (PMD)/pneumothorax (PNX) development in invasively ventilated patients with COVID-19-related acute respiratory distress syndrome (ARDS). MATERIALS AND METHODS: This is an observational, case-control study. From a prospectively acquired database, all consecutive invasively ventilated COVID-19 ARDS patients who underwent at least one baseline chest CT scan during the study time period (February 25th, 2020–December 31st, 2020) were identified; those who had tracheal lesion or already had PMD/PNX at the time of the first available chest imaging were excluded. RESULTS: 37/173 (21.4%) patients enrolled had PMD/PNX; specifically, 20 (11.5%) had PMD, 10 (5.8%) PNX, 7 (4%) both. 33/37 patients with subsequent PMD/PNX had Macklin effect on baseline CT (89.2%, true positives) 8.5 days [range, 1–18] before the first actual radiological evidence of PMD/PNX. Conversely, 6/136 patients without PMD/PNX (4.4%, false positives) demonstrated Macklin effect (p < 0.001). Macklin effect yielded a sensitivity of 89.2% (95% confidence interval [CI]: 74.6–96.9), a specificity of 95.6% (95% CI: 90.6–98.4), a positive predictive value (PV) of 84.5% (95% CI: 71.3–92.3), a negative PV of 97.1% (95% CI: 74.6–96.9) and an accuracy of 94.2% (95% CI: 89.6–97.2) in predicting PMD/PNX (AUC:0.924). CONCLUSIONS: Macklin effect accurately predicts, 8.5 days in advance, PMD/PNX development in COVID-19 ARDS patients. Pneumomediastinum (PMD) and pneumothorax (PNX) occur frequently in mechanically ventilated patients with coronavirus disease 2019 (COVID-19)-related acute respiratory distress syndrome (ARDS) [1] , with a reported incidence up to 24% in the first Italian pandemic wave [2] . COVID-19 ARDS patients are thought to be indeed more vulnerable to PMD/PNX occurrence as compared with patients with ARDS due to other than COVID-19 causes [1, 3] ; a higher than expected incidence of PMD/PNX was also observed in COVID-19 patients not requiring invasive mechanical ventilation [3] [4] [5] [6] . Furthermore, despite employment of lung protective ventilation strategies, these complications have difficult, non-standardized management, and are associated with increased risk of mortality [2, 7, 8] . Taken together, these findings corroborate the existence of a virus-induced frailty of airways tissue, resulting in an extensive, COVID-19-specific diffuse alveolar damage; microvascular thrombosis [9] , interstitial inflammation, as well as endothelial barrier disruption have been suggested as main contributors for such a condition [10] . There is indeed an urgent need for a tool able to objectively assess this lung frailty and therefore provide early risk stratification in terms of barotrauma susceptibility amongst mechanically ventilated COVID-19 ARDS patients. In this setting, we recently suggested a radiological predictor of spontaneous PMD/PNX, the so-called Macklin effect [2] , which is defined, on lung parenchyma windowed CT images, as a linear collection of air contiguous to the bronchovascular sheath [11] : 20 out of 21 patients with subsequent occurrence of spontaneous PMD/PNX were found to have Macklin effect on baseline computed tomography (CT) imaging, about ten days in advance of symptoms onset. Accordingly, we designed a larger study with the aim to define, on a broader cohort of patients, the exact accuracy of Macklin effect in predicting subsequent occurrence of overt PMD/PNX in mechanically ventilated COVID-19 ARDS patients. Our secondary objective was to describe topographical distribution of the Macklin effect within the lungs and to assess eventual associations with temporal interval before PMD/PNX occurrence. Under our Institutional Review Board-approved protocol (protocol number 34/int/2020; ClinicalTrials.gov number NCT04318366), we retrospectively identified all consecutive adult patients admitted to one of several intensive care units (ICUs) of San Raffaele Scientific Institute (Milan, Italy) for SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) infection and ARDS requiring invasive mechanical ventilation between February 25, 2020, and December 31, 2020 (n = 221). Patients who underwent at least one chest CT scan during their ICU stay, irrespective of contrast medium administration, were finally enrolled; of note, those who had documented tracheal lesion (n = 2) or already had PMD/PNX at the time of the first available chest CT imaging (n = 12) were excluded (Fig. 1) . In this cohort, occurrence of radiologically proven PMD/PNX was systematically recorded. Details on hospital organization and clinical management have been previously published [12] [13] [14] . Chest imaging studies (x-ray, CT scan), as well as contrast medium administration, were performed at the discretion of the attending physicians, according to clinical needs. Two experienced radiologists (D.P., G.G.) independently reviewed all selected baseline CT images searching for Macklin effect (Fig. 2 ); they were blinded to patients' symptoms and eventual outcome. Macklin effect was analysed in terms of presence/absence and topographical distribution within the lungs (adjacent to peripheral [segmental/ subsegmental] vs. central [lobar] bronchial branches). The reliability of agreement between the two readers was assessed using Fleiss' kappa (Fleiss' kappa values higher than 0.80 were deemed to be almost perfect). Macklin effect accuracy in terms of prediction of spontaneous PMD/PNX was measured in terms of sensitivity, specificity, positive and negative likelihood ratios (positive LR and negative LR, respectively), positive and negative predictive values (PPV and NPV, respectively) (Supplementary Appendix). Receiver operating characteristics (ROC) curve analysis was also performed and the area under the curve (AUC) computed. Dichotomous data were compared using χ 2 test; comparison of continuous variables between patients with peripheral and central distributed Macklin effect was evaluated using a Students' t-test. A p-value of less than 0.05 was considered statistically significant. Statistical analyses were performed with SPSS 25 (SPSS Inc./IBM, Armonk, NY). were included in our study. Overall, 37 patients (21.3%) suffered from spontaneous PMD/PNX during their ICU stay; specifically, 20 patients (11.5%) had spontaneous PMD, 10 spontaneous PNX (5.8%), whereas 7 (4%) experienced both. Fourteen patients (37.8%) required drainage tube placement, whereas the other 23 had conservative management. The inter reader agreement for Macklin effect detection was almost perfect (k value: 0.82) between the two radiologists. Thirty-three out of the 37 patients with subsequent occurrence of spontaneous PMD/PNX were found to have Macklin effect on baseline CT scan (89.2%, true positive patients), a median 8.5 days [range, [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] before the first radiological evidence of PMD/PNX. Conversely, 6 patients out of the 136 without PMD/PNX (4.4%, false positive patients) had the Macklin effect (p < 0.001). True and false negative rates were 95.6% (130/136) and 10.8% (4/37), respectively ( Table 1) Of note, the four false negative patients underwent baseline CT imaging significantly earlier when compared to true positive ones (23.2 ± 2.1 days before PMD/PNX occurrence vs. 7.5 days ±5.2, p < 0.001). Sub cohort analysis considering separately PNX and PMD demonstrated different performances of the Macklin effect in predicting these events. Specifically, amongst the 17 patients with PNX (alone or in association with PMD), no false negatives were detected Fig. 3c) (Table 1) . Finally, each patient requiring drainage tube placement was found to have Macklin effect on baseline CT imaging (14/14, 100%). The exact topographical distribution within the lung of Macklin effect was found to be peripheral in the majority of patients (25/33 [75.7%]). Peripheral distribution of Macklin effect was moreover associated with significantly higher temporal interval to the first radiological evidence of PMD/PNX when compared to central distribution (9.6 days ±6.4 vs. 1.4 days ±0.7, p < 0.001). Furthermore, the smaller the bronchovascular sheath involved, the longer the temporal advance (p < 0.001, R = − 0.896) (Fig. 4) . [b] Kaplan Meier curve demonstrating statistically significant difference between patients with "centrally-distributed" Macklin effect and those with "peripherally-distributed" Macklin effect in terms of temporal interval before pneumothorax/pneumomediastinum occurrence. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) We found that mechanically ventilated COVID-19 ARDS patients with Macklin effect on baseline CT imaging are to be considered at very high risk to develop clinically overt PMD/PNX (OR = 54.3). We also provided precise assessment of the accuracy of the Macklin effect in predicting subsequent PMD/PNX development, showing very high sensitivity and almost perfect specificity. Furthermore, Macklin effect demonstrated slightly different accuracy in predicting, separately, PNX and PMD, with the former having greater sensitivity values (no false negative results). Finally, we found a relationship between topographical distribution of Macklin effect on chest CT imaging and temporal interval to the first radiological evidence of PMD/ PNX: the smaller the bronchovascular sheath involved, the longer the temporal advance. A possible explanation to these findings lies in the pathophysiology of Macklin effect [15] [16] [17] . This is a three-step process: whenever a significant pressure gradient exists between air spaces and adjacent bronchovascular sheath (both in terms of increased alveolar and/or decreased interstitial pressure), alveolar rupture may occur [first step], resulting in air dissection along the pulmonary interstitium [second step] and air spreading into the mediastinum [third step]. Once the gas is in the mediastinum, it can then track to the cervical soft tissues (subcutaneous emphysema) or into the abdomen (pneumoperitoneum). A broad alveolar collapse might significantly reduce pulmonary compliance, possibly resulting in lung parenchyma rupture (PNX), not necessarily at the level of the alveoli primarily affected by the pressure gradient issue [16] . It follows that PNX represents the macroscopic outcome of a severe Macklin effect, and this is consistent with our data since we have observed no false positive results in patients with later PNX development (100% sensitivity, 100% NPV): a higher number of alveoli involved causes a larger air leakage along the pulmonary interstitium, resulting in a better recognizable Macklin effect on CT images. This observation also provides a compelling explanation for another finding: when dealing with clinically significant (namely, requiring intervention) PMD/PNX, Macklin effect was always detected on baseline CT scan. The topographical distribution (mainly peripheral) of Macklin effect observed in our population broadly differs from that previously reported in the literature [11, 18, 19] . In a cohort of 20 symptomatic patients with spontaneous (non COVID-19 related) PMD, for example, Okada and colleagues [19] pointed out a central distribution of Macklin effect in all patients. A possible reason for such a substantial discrepancy could lie in the different, quite novel design of the present study as compared with previous works, rather than in the different etiologies considered (COVID-19 vs. non . To the best of our knowledge, this is indeed the first study to systematically explore a possible prediction role for Macklin effect, looking for this sign before the actual occurrence of clinically overt PMD/PNX. Conversely, the existing, mainly radiological literature [11, [18] [19] [20] suggests Macklin effect as a diagnostic tool only (when PMD/PNX already occurred, with symptoms development), able to allow proper, non-invasive differentiation between respiratory PMD and other causes such as tracheobronchial/oesophageal injury. In this setting, pulmonary interstitial emphysema already dissected towards pulmonary hila, justifying a predominantly central Macklin effect. Timing of CT imaging was the main issue: the longer the time after the onset of Macklin effect, the less frequently it was detected peripherally; and this is consistent with our data, showing a relationship between topographical distribution of Macklin effect and temporal advance before the first radiological evidence of PMD/PNX. This assumption also provide a possible explanation for the four false negative patients we observed, since they all had baseline CT imaging three weeks before PMD/PNX occurrence, possibly before Macklin effect development. Taken together, these findings corroborate our novel hypothesis of Macklin effect as a sort of "radiological countdown" for PMD/ PNX development, capturing air leakage centripetal movement along the pulmonary interstitium. We believe that our findings may have potentially relevant research and clinical application. We recently completed a systematic literature review that confirmed the high mortality (greater than 60%) associated with development of barotrauma in COVID-19 ARDS patients [21] . In this context, early detection of Macklin effect could help to identify patients at high risk for overt/clinically relevant barotrauma. It is possible that this complication may be avoided by applying different ventilation strategies as compared with standard practice, for example by further reducing tidal volumes/airways pressures [22] , and considering early application of extracorporeal techniques if maintaining safer ventilatory parameters does not allow adequate gas exchange [23] . Use of extracorporeal membrane oxygenation (ECMO) without intubation has already been described in case reports, and may be a potentially interesting approach [23] [24] [25] . However, further studies are required to confirm the effectiveness of ultraprotective ventilation or ECMO in preventing overt barotrauma development in Macklin-positive patients, and that prevention of barotrauma actually translates in improved outcome. Of note, our findings may also be applied in non-COVID-19 ARDS, although this also requires further confirmation studies. The present study has several limitations, the most important ones being the relatively small sample size, and the lack of standardization regarding the timing of radiological examinations; external validation is also warranted. Moreover, one may argue that Macklin effect accounts for local peribronchiolar air leakage itself and that, once it is detected, subsequent development of clinically overt PMD/PNX is unavoidable. On that note, it is worth emphasizing that Macklin effect cannot be regarded as a full predictor of each pulmonary air leakage, but rather, in strict sense, as an early detector of lung frailty (following for example COVID-19 related diffuse alveolar damage). Accordingly, Macklin effect can be instead regarded as a predictor of clinically threatening occurrences like PMD/PNX since it allows early risk stratification in terms of barotrauma susceptibility amongst mechanically ventilated COVID-19 ARDS patients and, therefore, prompt deployment of ultraprotective ventilation strategies and ECMO. In doing so, the centripetal movement of any air leakage could be possibly "frozen" until full reabsorption, thus avoiding the occurrence of extensive lung damage (clinically overt PMD/PNX). In these terms, identification of Macklin effect may have a substantial clinical significance. In conclusion, Macklin effect is a reproducible, very accurate radiological sign able to predict clinically overt PMD/PNX development in mechanically ventilated COVID-19 ARDS patients with almost perfect specificity and very good sensitivity: patients with Macklin effect on baseline CT imaging are to be regarded extremely vulnerable to subsequent PMD/PNX occurrence. Further studies are needed to confirm these results in other (non COVID-19) clinical settings. None. Dr. Palumbo, prof. De Cobelli and prof. Landoni had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Conceptualization and methodology design: Palumbo, Landoni, Belletti, Guazzarotti, Zangrillo, De Cobelli. Acquisition, analysis, or interpretation of data: Palumbo, Landoni, Belletti, Guazzarotti, Pennella, Gritti, Marmiere. Drafting of the manuscript: Palumbo, Landoni, Belletti. Critical revision of the manuscript for important intellectual content: All authors. Statistical analysis: Palumbo. Supervision: Landoni, De Cobelli. None. Pneumomediastinum and subcutaneous emphysema in COVID-19: barotrauma or lung frailty? COVID-BioB Study Group. 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