key: cord-0757635-qx8emdlr
authors: Li, Wenhui; Urban, Stephan
title: Entry of hepatitis B and hepatitis D virus into hepatocytes: Basic insights and clinical implications
date: 2016-04-12
journal: J Hepatol
DOI: 10.1016/j.jhep.2016.02.011
sha: 8097952f917ec4d94c196d2c672dece4a64de1d1
doc_id: 757635
cord_uid: qx8emdlr

For almost three decades following the discovery of the human Hepatitis B Virus (HBV) the early events of virus infection (attachment to hepatocytes, specific binding to a receptor on hepatocytes) remained enigmatic. The gradual improvement of tissue culture systems for HBV has enabled the identification of viral determinants for viral infectivity and facilitated the discovery of the human sodium taurocholate co-transporting polypeptide (hNTCP) as a liver specific receptor of HBV and its satellite, the human Hepatitis Delta Virus (HDV). These findings are currently leading basic and clinical research activities in new directions. (1) Stable hNTCP-expressing cell lines have become a valuable platform to study the full HBV replication cycle from its native template, the cccDNA. (2) The suitability of NTCP complemented cell culture systems for high throughput screening approaches will facilitate identification of novel host factors involved in HBV replication (including those that determine the peculiar host specificity of HBV infection) and will enable identification and development of novel drug candidates for improved therapeutics. (3) Since NTCP is a major host-specific restriction factor for HBV and HDV, hNTCP-expressing animals provide the basis for future susceptible in vivo models. (4) The concept obtained with the entry inhibitor Myrcludex B demonstrates that NTCP is a suitable target for clinical interference with viral entry. This will foster further clinical approaches aiming at curative combination therapies.

Wenhui Li 1,⇑ , Stephan Urban 2,3,⇑

For almost three decades following the discovery of the human Hepatitis B Virus (HBV) the early events of virus infection (attachment to hepatocytes, specific binding to a receptor on hepatocytes) remained enigmatic. The gradual improvement of tissue culture systems for HBV has enabled the identification of viral determinants for viral infectivity and facilitated the discovery of the human sodium taurocholate co-transporting polypeptide (hNTCP) as a liver specific receptor of HBV and its satellite, the human Hepatitis Delta Virus (HDV). These findings are currently leading basic and clinical research activities in new directions. (1) Stable hNTCP-expressing cell lines have become a valuable platform to study the full HBV replication cycle from its native template, the cccDNA. ( 2) The suitability of NTCP complemented cell culture systems for high throughput screening approaches will facilitate identification of novel host factors involved in HBV replication (including those that determine the peculiar host specificity of HBV infection) and will enable identification and development of novel drug candidates for improved therapeutics.

(3) Since NTCP is a major host-specific restriction factor for HBV and HDV, hNTCPexpressing animals provide the basis for future susceptible in vivo models. (4) The concept obtained with the entry inhibitor Myrcludex B demonstrates that NTCP is a suitable target for clinical interference with viral entry. This will foster further clinical approaches aiming at curative combination therapies. Ó 2016 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.

The nature of the cellular receptor mediating HBV infection remained elusive long time after the discovery the virus [1] [2] [3] . Although some candidate molecules were found to bind to the envelope proteins of HBV, none of them ever demonstrated a virus receptor activity upon expression in non-susceptible cells, which is accepted as a strong criteria regarding whether a candidate is truly a functional virus receptor. Studies of the infection biology of HBV and its satellite virus hepatitis D virus (HDV), an RNA virus using HBV envelope proteins for its packaging and cellular entry, was hampered by the lack of a convenient cell culture system. Primary cultures of human hepatocytes (PHH) [4, 5] , primary tupaia hepatocytes (PTH) [6] [7] [8] , and a hepatoma cell line, HepaRG, after differentiation with DMSO [9] , were the only cells susceptible to HBV and HDV. PHH and PTH are scarce and their susceptibility to the virus varies depending on the source as well as isolation and culture procedures. HepaRG cells require a 2-week differentiation period before cells become susceptible. Nonetheless, elegant work using these cell culture systems over the past decades has revealed some critical aspects of HBV entry [10] : (1) Infection of HBV exhibits extraordinary species and organ specificity, indicating existence of a highly specific interaction between the virus and the hepatocytes; (2) two of HBV three envelope proteins, namely the small (S) and large (L) envelope protein, but not the middle (M) protein are responsible for HBV entry [11, 12] ; (3) the antigenic loop (AGL) of S protein [13] and the preS1 domain of L-protein are essential for HBV infection [14] ; (4) the N-terminal region of preS1 domain requiring an uninterrupted sequence of 75 amino acids (genotype D) [15] with a myristoylation modification at the N-terminus [16, 17] critically contributes to infectivity, among which amino acid residues 9-16 are of vital importance [18] [19] [20] . The corresponding lipopeptide of this domain blocks viral infection in vitro and in vivo [19] [20] [21] [22] . The preS1 lipopeptide as a natural ligand is useful to identify the specific receptor on hepatocytes. However, it was not an easy task to find its binding partner on hepatocytes and several nonfunctional molecules have been described [10] . Successful discovery of the bona fide receptor sodium taurocholate co-transporting polypeptide (NTCP) was achieved using an innovative preS1 peptide ligand combined with proteome analysis of PTH and highly efficient tracing and purification processes [23] . Biochemical studies confirmed the interaction between preS1 and the liver specific bile acids transporter. Remarkably, transfection of HepG2 cells with NTCP expression DNA vector confers susceptibility to this otherwise non-susceptible cell [23] . This finding was quickly confirmed by several important studies, including a study demonstrating that human NTCP can confer susceptibility to HDV pseudotyped with the envelope proteins of bat hepadnavirus [24] , woolly monkey HBV (WMHBV) also exploits NTCP for viral entry in tupaia hepatocytes [25] and HBV/HDV exploit NTCP for species-specific entry into hepatocytes [26] . Discovery of the receptor has also engendered new perspectives on HBV and its associated diseases [27] .

NTCP is coded by the SLC10A1 gene. It is a bile acid transporter, responsible for hepatic uptake of the majority of conjugated bile acids from blood, and plays a critical role in bile acids homeostasis and enterohepatic circulation [28] . The expression of NTCP and its subcellular distribution is subjected to precise regulation at multiple levels. NTCP contains up to nine transmembrane segments embedded in the cell membrane; its structure remains to be elucidated. Interestingly, although the NTCP gene is conserved in mammals, disrupting NTCP in mice in short term (ca. 2 months) did not result in significant abnormalities in the animals but led to elevated serum bile acids, in particular conjugated ones [29] . A single point mutation of R252H of human NTCP interferes with its surface expression on hepatocytes. Recently, a five-year old girl bearing this mutation was identified and she exhibited mild hypotonia, growth retardation, delayed motor milestones but no severe health problem [30] . Therefore, although the long-term consequence of disrupting NTCP needs more studies, the physiological role of NTCP can probably be compensated by other, yet not defined mechanism(s), and redundant pathways may operate in the absence of NTCP. Whether there is another receptor(s) for HBV entry is not known at present, however several lines of evidence support that NTCP is well suited as a key receptor for HBV (Fig. 1) . It is predominantly expressed in hepatocytes [31] , as also shown by the hepatotropism of its preS-ligand in vivo [32] . This is consistent with the high liver specificity of HBV. Furthermore NTCP resides on the sinusoidal side of hepatocytes [33] [34] [35] , which is in line with the blood transmission of the virus. There is only very low or no expression of NTCP in hepatocarcinoma cell lines like HepG2 or HuH7 and the expression of NTCP rapidly decreases after isolation of primary hepatocytes from animals [23, [36] [37] [38] . These two features at least partially explain why normal liver cancer cells are resistant to HBV infection and why the susceptibility of primary hepatocytes persists for only a few days after isolation.

Interestingly, a human population study on 1899 HBV patients from southern China (Guangdong) showed that a single mutation of NTCP, S267F ((c.800G>A, rs2296651) on SLC10A1), is associated with resistance to chronic hepatitis B [39] . The S267F is a mutation found mainly in Asian populations, with a minor frequency of ca. 10 % [40] [41] [42] . In cell cultures the mutation abolished taurocholate (TC) transport and over expression of S267F NTCP in HepG2 cells failed to support genotype D HBV and HDV infection [41] . The marked protection of this mutation against HBV infection at population level strongly argues that NTCP is a major viral receptor in human. A more recent genetic association study based on 3801 chronic hepatitis B (CHB) patients from Taiwan confirmed that the same mutation confers resistance to HBV infection and is independently associated with decreased risk of cirrhosis and HCC [42] . It is currently unclear how the mutation would reduce the risk of cirrhosis and HCC.

Interestingly, there are five HBV patients with homozygous S267F NTCP mutation in the Guangdong cohort [39] the Taiwan study also found five of such HBV patients [42] . How these patients were infected is an interesting and important question. There are several possibilities: first, cell surface molecules other than NTCP could mediate the viral infection; second, the virus was adapted to S267F-NTCP; third, the patients were genetically mosaic meaning some of their hepatocytes might still have wildtype NTCP. Clearly more work is needed to clarify these possibilities and related studies would help to deepen our understandings of HBV infection. experimental animals. However, once the HBV genome is delivered to the monkey hepatocytes, the virus can robustly replicate indicating that these cells are able to form cccDNA and replicate the virus [43] . Mouse hepatocytes bearing an HBV transgene can produce infectious virions but are unable to form cccDNA [44] . These observations support the hypothesis that the species restriction of HBV is largely at the entry level. Entry restriction is common in cross-species transmission of many viruses, for example: the infection of influenza virus among mammals and birds heavily depends on the existence of sialic acids (SA)-a-2,6-Gal-terminated saccharides or (SA)-a-2,3-Gal-terminated saccharides receptor [45] ; and animal transmission of coronaviruses, including SARS-CoV and MERS-CoV is controlled by their receptors [46] . Whether NTCP is responsible for the species specificity at entry level was first examined by assessing HBV infection mediated by NTCP chimeras from crabeating monkey (Macaca fascicularis) and human [23] . A small motif covering residues 157-165 of NTCP was identified to be critical for preS1binding and HBV infection, highlighting the critical role of NTCP in mediating HBV infection Bile acids [23, 26] . The observation that cynomolgus monkey hepatocytes cannot bind the preS1lipopeptide in vitro and in vivo strongly supported this finding [32, 34] . The key role of residues 157-165 for HBV infection in humans has also been supported by the recent CHB population study in Taiwan, in which no mutation was found in this region in all CHB patients [42] . Interestingly, naturally occurring transmissible HBV infection (genotype D) was reported among M. fascicularis from Mauritius Island, however, the viral titre was very low and more evidence is needed to support that HBV undergoes a full life cycle in these monkeys [47] .

The receptor-addressing preS1-lipopeptide can bind to primary hepatocytes from HBV nonsusceptible animals, such as mouse, rat and dog [32, 34] . This finding was surprising and indicated the resistance of these animals to HBV at entry level was less likely due to the lack of a specific binding partner of the virus on the hepatocytes. Accordingly, either the binding could not lead to a productive infection, or there is difference between the isolated preS1 peptide versus the same region residing on the virions. Extensive mapping studies revealed that amino acid residues 84-87 in the first extracellular loop of NTCP restricted mice from infection of HBV at the entry level. Replacing three of these four amino acids with their human counterpart effectively render mouse NTCP a functional receptor for HBV in HepG2 cells [26, 48] . Consistent with the notion that NTCP poses the major barrier for viral infection of mice, suckling C57BL/6 mice bearing human NTCP are susceptible for HDV infection and the virus successfully undergoes most of its life cycles in the animal [49] . However, although mouse liver cells like Hepa1-6, Hep56.1D, mH274#26 supplemented with human NTCP support HDV infection, they failed to support HBV infection. These studies indicate that factors other than NTCP may be also involved in HBV infection. Although the nature of the factor(s) is still unknown, HBV infection studies based on cell fusion assays of HepG2 and mouse cells indicated that infection in mouse cells is limited by the lack of a host cell dependency factor [50] . Interestingly, a study recently indicated that the stability of viral nucleocapsids may be a determinant for cccDNA formation in murine hepatocytes (AML12) [51a] . Interestingly, this cell line, when supplemented with hNTCP supports infection with HBV [51b]. It will be important to further identify cellular factors to successfully establish a small animal model for HBV infection. On the other hand, one also has to be aware that viral infection in tissue culture can be influenced by the variables among different cell clones, the dosage of the inoculating virus and others factors that are different from the physiological conditions, therefore only viral infection of animals with defined gene alternations can provide definitive answer to the question.

NTCP complemented HepG2 cells provide a valuable platform for screening new host factors and developing antivirals against HBV/ HDV infection HepG2 cells complemented with human NTCP provide an easy accessible system for HBV/HDV infection. The system is amendable for high throughput screening procedure, thus enabling studies of systemic identification of new factors involved in HBV/HDV infection and developing new antivirals. Targeted screening with HepG2-NTCP cells has identified glypican 5 (GPC5) as an entry factor for HBV/HDV [52] ; viral infection based assay has found some promising drug candidates in blocking HBV/HDV infection [53, 54] . HBV can undergo its whole life cycle in HepG2-NTCP cells, from entry to establishment of the infection, and at a low efficiency of releasing of progeny virions. The covalent closed circular DNA (cccDNA) of HBV is the reservoir of chronic infection and is widely believed to be the major obstacle for eliminating the infection [55, 56] . HepG2-NTCP cells support cccDNA formation from incoming virions and are less efficient for the replenishment of cccDNA via intracellular recycling. Interestingly, although the carcinomatous nature of HepG2 cell is a concern for studies using HepG2-NTCP cells, a recent study indicates the epigenetic regulations of cccDNA in the cells are similar to those observed in PHH [57] .

Therapeutic interference by entry inhibitors is an attractive clinical concept since it inhibits infection at its earliest step thereby preventing the establishment of viral genomes (e.g. cccDNA) in naïve or regenerating host cells [58] . Entry inhibition in persistently infected patients is presumably most efficient when virus spread requires entry of progeny virus into naïve cells via a receptor-mediated pathway. In addition, a fast replenishment of infected cells by naïve cells would limit the time of treatment to clear the infection. For HIV it has been shown that a direct spread from one cell-to-another can occur which might explain the therapeutic limitation of entry inhibitors [59, 60] . Nevertheless, Fuzeon Ò and maraviroc, two approved entry inhibitors, show antiviral activity in patients [60] [61] [62] . For HBV it has been demonstrated that spread in PHHtransplanted uPA severe combined immunodefficient (SCID) mice was completely blocked by the Key point hNTCP-expressing HepG2 cell lines allow high throughput approaches for drug identification.

NTCP-specific entry inhibitor Myrcludex B. This indicates that intrahepatic spread greatly depends on an NTCP-mediated entry of progeny virus into naïve hepatocytes [63] . Moreover, cccDNA-dissemination in the liver of these animals is abrogated by cell proliferation [64] , indicating that HBV depends on an extracellular virus cycle and works poorly by transferring the cccDNA template via an intercellular pathway into progeny cells. Thus, entry inhibition for HBV may become clinically important in order to block cccDNA reformation during either natural or immune-mediated/induced hepatocyte turnover.

Entry inhibition can be achieved by targeting viral structures (e.g. the neutralisation of envelope proteins at the viral surface, which are involved in receptor binding). In the course of the resolution of an acute infection, neutralising antibodies fulfil this crucial role and are therefore indispensable to clear the virus. Accordingly, antibodies recognizing envelope proteins have been developed for therapeutic purposes [65] [66] [67] . Currently, neutralising antibodies are preferentially applied in prophylactic clinical settings. For HBV and HDV such antibodies prevent reinfection of liver transplants in infected patients, are effective in post exposure prophylaxis or the prevention of mother-child transmission [68] . Long-term administrations of HBV neutralising antibodies in chronically infected patients have not been performed so far.

An alternative strategy to inhibit viral entry takes advantage of drugs that target cellular receptors/co-receptors. This approach is relatively new but has been shown to work in HIV infected patients [60] . Their mode of action is based on the sustained inhibition of de novo formation of viral templates. Although they are not curative in HIV infected patients, they theoretically bear this potential even without the involvement of adaptive immune responses. This has been recently demonstrated in an immunedeficient animal model for hepatitis C virus (HCV) infection [69] . However, the duration of treatment to gain virus elimination depends on the natural turnover rate of infected cells, the half-life time of the virus and the presence of possible viral reservoirs, which have been established in the host. Importantly, immune responses that are directed against infected cells may dramatically enhance the clearance of infected cells. Accordingly, entry inhibition may be highly synergistic, when combined with immune therapeutic approaches.

Whether a virus receptor is a suitable ''drugable target" depends on different aspects. Firstly, virus spread within an organism should be subject to receptor-mediated de novo infection and should not primarily proceed by cellto-cell transmission of viral genomes. Secondly, since virus receptors often serve important cellular functions, the drugs should not completely mess up the natural function of these molecules (clinical manageability). This can be achieved if the targeting drug blocks the receptor function at an IC 50 below the concentration required for inactivation of its cellular function. Such a ''therapeutic window" would allow drug dosing, which is clinically acceptable even if the target molecule displays an essential function. Alternatively, the receptor may not be an essential protein and its function might be compensated by salvage pathways. Finally, the interaction of the drug with the receptor should be highly specific in order to avoid ''off-target" effects.

HBV specifically targets hepatocytes in the human liver with very high efficacy [70] . This hepatotropism is related to specific interactions of the viral envelope proteins with host factors, including heparan sulfate proteoglycans (HSPG) [71] [72] [73] and NTCP (see above). It has been shown that known substrates of NTCP as well as HSPGs interfere with HBV and HDV infection. These substances can be subdivided in different groups: firstly, natural or artificial substrates of HSPG like heparin, highly sulphated dextrans, suramin or negatively charged polymers [71] [72] [73] . They shall not be further discussed here. Secondly, substrates of NTCP, like the conjugated bile acid TC or glycocholic acid [26, 48, 74, 75] . These substrates but also bile salt homologues are transported by NTCP in a sodium dependent manner [28] . They bind into a pocket of the outward conformation of NTCP and are released (together with two sodium ions) after a substantial structural change to the inward conformation into the cytoplasm of the hepatocyte [53] . At high concentrations, conjugated bile salts inhibit HBV and HDV infection [41, 76] . The concentrations needed for infection inhibition are above the normal bile salt levels in healthy and HBV infected individuals, which probably explains the lack of therapeutic effects of bile salts in HBV/HDV infected patients [76] .

In addition to endogenous substrates, xenobiotics and certain drugs have been shown to use the transporter function of NTCP to enter hepatocytes [77] . Some of these molecules have been developed as drugs and have been discovered as NTCP addressing agents in the course of their pharmacokinetic characteristics. One example is ezetimibe, which is used as an inhibitor of the primary cholesterol transporter NPC1L1 in the intestinal lumen. It is also a substrate of NTCP [28] and has been later shown to interfere with HBV and HDV entry [78] . These substances have in common that the mechanism of HBV receptor inhibition is coupled to their ability to bind the substrate in the bile salt pocket and thereby block the receptor to interact with the virus. Evidence for that comes from the finding that determinants for viral entry and bile salt transport functionally overlap [41] . Accordingly, the reported K D s of transporter activity and HBV inhibition activity are comparable. In the future, it will be interesting to see whether small molecules that bind NTCP in its substrate pocket (e.g. bile salt analogues that might covalently bind to the transporter), could act as irreversible deadend inhibitors.

The third class of NTCP inhibitors are peptidic substrates, which bind, but are presumably not transported by NTCP. A prototypic member of this family is cyclosporin A, which was known as an NTCP-inhibitor before it was described as an HBV receptor [79] . Its antiviral activity is not restricted to the natural product cyclosporine A, but also holds up for other derivatives including non-immunosupressive variants like alisporivir [74, 80] . Like for Myrcludex B (see below), the IC 50 s of cyclosporin A to inhibit HBV and HDV infection is significantly lower compared to its inhibitory effect on taurocholate (TC) uptake, indicating that a therapeutic effect against HBV might be achieved at non-NTCP-saturating concentrations [74] . However, since cyclosporins are not specific for NTCP and inhibit (in addition to modulate intracellular cyclophilins) other bile salt transporters like organic anion-transporting polypeptide (OATP) at even lower concentrations [81] their therapeutic use for CHB and chronic HDV infection might be limited.

HBV L-protein derived lipopeptides as highly specific inhibitors of NTCP Due to the high sequence homologies in their receptor binding site, the N-terminal preS1domains of primate HBVs, like WMHBV or Gorilla HBV and even the recently discovered Bathepatitis B viruses are assumed to specifically bind their respective host Ntcps. This interaction only occurs after a preceding interaction to and possibly activation by HSPGs. Remarkably, virions do not expose the N-terminal preS-1 NTCP binding site 9-NPLGFFP-15 on their surface [53] . Accordingly, HBV may hardly be recognized and inhibited by monoclonal antibodies recognizing this motif [82] . Moreover, chronically infected patients with high loads of preS-containing virions and subviral particles do not show elevated serum bile salt levels indicating that the NTCP may not or only partially blocked by the presence of these particles [83] . In contrast, synthetic preS1-derived lipopeptides that contain the NPLGFFP-motif are potent NTCP-binders and block the bile salt transporter function [26, 41, 75] . Such peptides have been characterized extensively with regard to their specific activities and the role of the fatty acid moiety which is required for anchoring the peptide in the membrane close to the NTCP binding site [9, [18] [19] [20] [21] [22] [84] [85] [86] . A lead substance of these peptides, which is presently in clinical development, is Myrcludex B. It efficiently inhibits HBV and HDV infection of hepatocytes at already picomolar concentrations [18] . Notably, Myrcludex B as a peptidic drug of 47 amino acids contains preSspecific epitopes including the essential receptor binding motif 9-NPLGFFP-15. It also contains Cterminally located epitopes (e.g. 19-DPAF-22), which may induce highly neutralising antibodies [87] . Accordingly, Myrcludex B therapy may induce anti-preS-specific antibodies that might help to eliminate HBV or HDV from the liver.

In contrast to the small substrate molecules mentioned above, preS-derived lipopeptides (including Myrcludex B, as the clinically used lead substance) are allosteric inhibitors of NTCP, which irreversibly bind and inactivate the HBV receptor function of the molecule. These peptides also inhibit the bile acid transporter function, but only at ca. 500-fold higher concentrations [26, 75] . Functional recovery of NTCP is slow and probably only possible when the preS/receptor complex is dissociated after endocytic turnover or replacement by de novo synthesis [21] . Whether functional recycling of NTCP after formation of a preS-complex can occur is presently unknown. The reason for the remarkable differences in the IC 50 s of e.g. Myrcludex B between bile salt transporter (IC 50 ca. 47 nM) activity and HBV/HDV infection inhibition (ca. 0.1 nM) is still unclear. However, a plausible assumption is that the HBV L-protein forms multimeric (e.g. trimeric) complexes with NTCP (as it has been shown for many other enveloped viruses). Such a complex could be functionally inactivated, if only one out of three binding sites is occupied by the peptidic fragment. In fact, NTCP has been shown to form multimeric complexes [88] . From a therapeutic point of view, this allows to inactivate the receptor function of NTCP at doses that do not significantly affect its natural function as a bile salt transporter.

A phase I safety trial revealed that Myrcludex B, the first in class entry inhibitor addressing the NTCP-receptor of HBV was well tolerated in healthy volunteers at intravenously applied dose of up to 20 mg. Pharmacokinetic studies revealed that doses of >10 mg subcutaneously resulted in NTCP saturation >80% for at least 15 h (Blank The trial performed in HBeAgnegative HBV monoinfected patients shows a reduction in HBV serum levels by a mean of 10 1.45 within 12 weeks of treatment in the 10 mg arm. Assuming that Myrcludex B only interferes with de novo infection of cells and that the rate of virus production per infected cells remains unchanged, this indicates a substantial reduction of the number of infected cells during treatment. However, this has to be proven directly in future studies by immunohistochemistry of liver biopsies. In the sub study of 24 HDV co-infected HBV patients (Bogolomov et al., under revision) Myrcludex B was applied either alone at 2 mg daily, or in combination with PegIFN for 24 weeks. Myrcludex B was well tolerated in all patients, with only slightly increased conjugated bile salt levels. Within 24 weeks HDV RNA declined by >1 log in all cohorts and even became negative in two patients each in the Myrcludex B and PegIFN control arm. Remarkably, negativation was observed in five of seven evaluable patients of the Myrcludex B/ PegIFN combination arm, indicating a synergistic effect both drugs.

Taken together these first proof of concept studies using Myrcludex B demonstrate that targeting NTCP as a key molecule of hepatocyte specific entry of HBV is a feasible clinical concept in future therapeutic approaches aiming at the functional cure of chronic Hepatitis B and Hepatitis D. It will be interesting to see if long-term inhibition of viral entry results in HBsAg loss or anti-HBsAg seroconversion and whether combination with immunomodulatory drugs like IFNalpha can induce and/or accelerate such a process. 

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Analysis of the serum bile Acid composition for differential diagnosis in patients with liver disease

An anti-viral peptide derived from the preS1 surface protein of hepatitis B virus

Inhibitors of hepatitis B virus attachment and entry

Mapping of the hepatitis B virus pre-S1 domain involved in receptor recognition

Attachment sites and neutralising epitopes of hepatitis B virus

Homo-and hetero-dimeric architecture of the human liver Na(+)-dependent taurocholate co-transporting protein

Wenhui Li is a co-inventor of a patent application for compositions and uses of functional receptor for HBV/HDV virus. Stephan Urban is coapplicant and co-inventor of patents protecting Myrcludex B.