key: cord-0756919-dnwye2py
authors: Hensley, Matthew K; Bain, William G; Jacobs, Jana; Nambulli, Sham; Parikh, Urvi; Cillo, Anthony; Staines, Brittany; Heaps, Amy; Sobolewski, Michele D; Rennick, Linda J; Macatangay, Bernard J C; Klamar-Blain, Cynthia; Kitsios, Georgios D; Methé, Barbara; Somasundaram, Ashwin; Bruno, Tullia C; Cardello, Carly; Shan, Feng; Workman, Creg; Ray, Prabir; Ray, Anuradha; Lee, Janet; Sethi, Rahil; Schwarzmann, William E; Ladinsky, Mark S; Bjorkman, Pamela J; Vignali, Dario A; Duprex, W Paul; Agha, Mounzer E; Mellors, John W; McCormick, Kevin D; Morris, Alison; Haidar, Ghady
title: Intractable Coronavirus Disease 2019 (COVID-19) and Prolonged Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Replication in a Chimeric Antigen Receptor-Modified T-Cell Therapy Recipient: A Case Study
date: 2021-01-28
journal: Clin Infect Dis
DOI: 10.1093/cid/ciab072
sha: b026e6c645e20b5ded2c9e699052d0f0be37854e
doc_id: 756919
cord_uid: dnwye2py

A chimeric antigen receptor-modified T-cell therapy recipient developed severe coronavirus disease 2019, intractable RNAemia, and viral replication lasting >2 months. Premortem endotracheal aspirate contained >2 × 10(10) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA copies/mL and infectious virus. Deep sequencing revealed multiple sequence variants consistent with intrahost virus evolution. SARS-CoV-2 humoral and cell-mediated immunity were minimal. Prolonged transmission from immunosuppressed patients is possible.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in patients with hematologic malignancies results in poor coronavirus disease 2019 (COVID-19)-related outcomes [1] . Recipients of chimeric antigen-receptor-modified T-cell therapy (CAR T-cell) are at risk for severe COVID-19 because of chronic B-cell aplasia and hypogammaglobulinemia due to "on-target/off-tumor" effects of CAR T cells [2] , which occur when CAR T cells kill normal B cells that express the CAR T-cell target antigen. Conditioning regimens for CAR T-cell therapy can also cause lymphopenia and diminish B-and T-cell function. Although persistence of SARS-CoV-2 RNA in respiratory specimens is not thought to represent infectiousness [3, 4] , it is possible that CAR T-cell recipients can persistently shed infectious SARS-CoV-2. Similarly, SARS-CoV-2 RNAemia, which correlates with disease severity [5] , is thought to be short-lived [6] but may be more protracted in immunosuppressed hosts.

We present a case of prolonged SARS-CoV-2 infection in a patient with multiple myeloma who received CAR T cells targeting the B-cell maturation antigen (BCMA), which is universally expressed on malignant plasma cells and on some normal plasma cells and mature B cells and is involved in plasma cell survival and B-cell differentiation into plasma cells [7] [8] [9] [10] . We characterize viral persistence, intrahost viral evolution, and immune profiles from longitudinal samples and demonstrate that the patient experienced high-level SARS-CoV-2 RNAemia and viral replication for >2 months, viral diversification, and massive lung infection before dying from the infection.

The patient was a 73-year-old man with treatment-refractory multiple myeloma. He had undergone an autologous hematopoietic cell transplant 2 years prior but developed recurrent disease and therefore underwent anti-BCMA CAR T-cell therapy after fludarabine/cyclophosphamide lymphodepletion. Nasopharyngeal (NP) swab reverse-transcription polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 was negative 17 and 2 days before CAR T-cell therapy. He received tocilizumab for cytokine release syndrome, a known toxicity of CAR T-cell therapy [2] , but not corticosteroids. He was discharged home in stable condition. Twelve days after discharge (and 25 days after the CAR T-cell infusion), he was admitted to the intensive care unit (ICU) with 2 days of a productive cough, dyspnea, anorexia, and lightheadedness. Temperature was 37.1°C; oxygen saturation was 86% on room air. Laboratory evaluation showed a white blood cell (WBC) count of 3.5 × 10 9 cells/L (normal range, 3.8 × 10 9 -10.6 × 10 9 ), with an absolute lymphocyte count (ALC) of 0.7 × 10 9 cells/L (normal range, 0.8 × 10 9 -3.65 × 10 9 ) and an absolute neutrophil count (ANC) of 2.6 × 10 9 cells/L (normal range, 2.24 × 10 9 -7.68 × 10 9 ). NP swab RT-PCR testing was positive for SARS-CoV-2 RNA (day 0); cycle thresholds were 20.1 and 21.5 for the nucleoprotein 1 (N1) and envelope (E) genes, respectively (Figure 1-I). Chest radiography revealed bibasilar and midzone opacities. He received convalescent plasma (day 2) and remdesivir (days 5-10). During this hospitalization, he had escalating oxygen requirements, necessitating the use of noninvasive positive pressure ventilation on day 5. He never required intubation, and his oxygen requirements gradually improved over the following week. On day 14, he no longer needed supplemental oxygen and was discharged in stable condition on day 17, with minimal residual dyspnea. Follow-up SARS-CoV-2 NP RT-PCR testing remained positive (days 15, 26, and 37). Cycle threshold values were unavailable.

Forty-one days after being diagnosed with COVID-19, he was readmitted to the ICU with a 1-week history of weakness and 4 days of progressively worsening dyspnea, a minimally productive cough, and diarrhea. During the 24 days between hospital discharge and readmission, he had not left his residence except for obtaining outpatient SARS-CoV-2 PCR testing. Temperature was 36.9°C. Oxygen saturation was 81% on room air, and he was started on heated high-flow supplemental oxygen with improvement in his saturation to 97%. WBC count and ANC were normal, but ALC was 0.1 × 10 9 cells/L. Lymphocyte subset testing showed zero CD19 + B cells and 32 CD3 + T cells/μL (normal range, 856-2669/μL). Chest computed tomography demonstrated bilateral ground glass opacities (Figure 1 -I).

Since the literature at the time of the patient's presentation suggested that prolonged PCR positivity indicated the presence of noninfectious RNA [3, 4] , he initially received no specific treatments for COVID-19. Instead, an extensive evaluation was sent to identify other infections, which included blood cultures (grew Escherichia coli that rapidly cleared with piperacillin-tazobactam) and the following tests, all of which were negative: urine Legionella antigen, plasma PCR for adenovirus, cytomegalovirus, and Epstein-Barr virus, serum for Aspergillus galactomannan and β-D-glucan, non-SARS-CoV-2 respiratory virus PCR, and stool pathogen PCR. He required pressor support on day 50 for hypotension, and his respiratory status continued to worsen. He was intubated on day 55 and required mechanical ventilation with 80%-100% fraction of inspired oxygen for the remainder of his hospital stay. SARS-CoV-2 NP RT-PCR testing remained positive on day 55; cycle thresholds were 13.3 and 16 for the N1 and E genes, respectively. Bronchoalveolar lavage fluid from day 55 grew rare Klebsiella pneumoniae, which was not thought to be significantly contributing to his condition but was treated with meropenem, with no improvement in his respiratory status.

Because no etiology aside from COVID-19 could be identified, he received another course of convalescent plasma (day 58) and dexamethasone (days 63-74) (Figure 1-I) . He was unable to receive additional remdesivir due to limited availability. Serum from days 45, 56, and 71 was negative for SARS-CoV-2 immunoglobulin G (IgG) and immunoglobulin A (IgA) (Euroimmun assay [11] ). Despite myeloma biomarkers showing an excellent response to CAR T-cell therapy, his family ultimately decided to focus on comfort measures because of nonresolving respiratory failure. The patient passed away on day 74 after COVID-19 diagnosis.

The patient was enrolled in the University of Pittsburgh's Acute Lung Injury Registry and Biospecimen Repository (institutional review board number STUDY19050099). SARS-CoV-2 RNA quantification was performed using a sensitive (limit of detection, 3 copies/reaction) quantitative RT-PCR (qRT-PCR) assay on plasma (days 4, 9, 13, 16, 67, and 71) and in endotracheal aspirate (ETA) fluid (from day 72). SARS-CoV-2 Spike-specific deep next-generation sequencing (NGS) was performed on plasma (days 4, 13, 67, and 72) and ETA fluid (day 72). Virus isolation from plasma (days 4, 65, and 71) and ETA fluid (day 72) was attempted on Vero E6 cells, followed by indirect immunofluorescence and dideoxy sequencing of the Spike (S) gene. Anti-SARS-CoV-2 IgG antibody titers directed against the S-protein receptor binding domain (RBD) using an indirect enzyme-linked immunosorbent assay (ELISA), competitive ELISA using human ACE2, and pseudovirus neutralization assays were performed with samples from days 4, 9, 13, 16, and 72. Plaque reduction neutralization assays on live SARS-CoV-2 (laboratory strain) were also performed (days 4, 65, and 71). To characterize T-cell and B-cell responses, single-cell RNA sequencing (scRNA-seq) was performed on peripheral blood mononuclear cells (PBMCs) isolated from blood obtained on days 4 and 9. SARS-CoV-2 S-specific T-cell responses were determined by flow cytometric quantification of T-cell frequencies with intracellular staining of interferon-γ and CD107a, following co-culture with S-protein and nucleocapsid protein peptide pools. Assay details can be found in the Supplementary Materials.

High levels of SARS-CoV-2 RNA were detected in all plasma samples (Figure 1 -I). RNAemia was greatest on day 4 (126 792 copies/mL) and showed a >10-fold decrease by day 9 (8100 copies/mL), after administration of convalescent plasma and remdesivir. RNAemia remained readily detected but <100 000 copies/mL on day 13 (11 508 copies/mL), day 16 (18 000 copies/ mL), and day 67 (14 720 copies/mL), then increased to 101 800 copies/mL on day 71 during administration of dexamethasone. More than 20 billion copies/mL of SARS-CoV-2 RNA (2.78 × testing and clinical immunoglobulin G and immunoglobulin A testing. The bottom graph shows serial plasma SARS-CoV-2 RNA quantification obtained using a research quantitative RT-PCR assay, with viral quantification from endotracheal aspirate fluid using research assays (quantitative RT-PCR and infectious viral titer). Inset shows a coronal 10 10 copies/mL) were detected by qRT-PCR in a serially diluted ETA sample from day 72.

Six different SARS-CoV-2 sequence variants were identified in longitudinal plasma and ETA fluid between days 4, 13, 67, and 72 ( Figure 2 and Supplementary Methods). On day 4, plasma viral S sequences matched the SARS-CoV-2 GH clade (containing D614G) that was circulating in Pittsburgh at the time. However, by day 13, while the patient was still in the hospital, additional mutations were detected, including R190K and G1124D substitutions, which were previously observed in 0.005% (n = 9/189 163) and 0% (n = 0/189 163), of GISAID SARS-CoV-2 sequences, respectively. Comparison of sequences between days 13 and 67 demonstrated the emergence of several additional mutations, such as a Y144 deletion and a D215G substitution, which were later identified in the United Kingdom (UK) and South African variants, respectively, several months after the patient's death [12, 13] . We also identified an N501T substitution on day 67, which was recently shown to enhance binding affinity to the ACE2 receptor [14] . Interestingly, a substitution at the same location (N501Y) is currently circulating in the UK and South Africa [12, 13] . Finally, mutations continued to emerge between days 67 and 72 (eg, H146 deletion). The longitudinal emergence of multiple different and novel sequence variants is indicative of intrahost evolution of SARS-CoV-2.

Infectious SARS-CoV-2 was recovered from the day 72 ETA sample ( Figure 1 -II) with a titer of 1.125 × 10 6 plaque-forming units (PFU)/mL. Numerous SARS-CoV-2 virions were detected by electron microscopy in the ETA sample (Figure 1-III) . Dideoxy sequencing of the S-gene of cultured virus showed mutations consistent with those identified by NGS (eg, D614G substitution, Y144 deletion, and H146 deletion). Viral isolation from plasma was unsuccessful despite the presence of SARS-CoV-2 RNA.

Analysis of PBMCs (days 4 and 9) by immunophenotyping and scRNA-seq demonstrated absence of B cells and near total depletion of T cells, consistent with lymphodepleting chemotherapy with fludarabine and cyclophosphamide (Supplementary Figure 2B) . The scRNA-seq profiling showed interferon-stimulated genes in the monocyte lineage suggestive of a viral infection. We evaluated SARS-CoV-2-specific T-cell responses from days 4 and 67. The day 4 sample contained too few live T cells (<1%) to assess. The day 67 PBMC sample showed somewhat higher levels of live T cells (9.2%), with 1.72% and 0.79% of CD8 + T cells expressing the cytotoxicity marker CD107a following stimulation with S protein and nucleocapsid peptide pools, respectively, suggesting that a small fraction of the T cells were able to react to SARS-CoV-2 antigens ex vivo (Supplementary Figure 3) . However, the low number of events (<5000 per sample) acquired by flow cytometry prevents any conclusions from being drawn from these analyses. Despite the administration of convalescent plasma on days 2 and 58, no IgG antibody targeting the S-protein RBD was detected at any time point, nor was there any antibody that competed with human ACE2 binding to RBD, possibly because the convalescent plasma used had a low titer of SARS-CoV-2 antibody, or there was rapid clearance of antibody bound to very higher numbers of virions (Supplementary Table 2 ). Additionally, SARS-CoV-2 plaque reduction neutralization assays showed no plaque reduction in the day 4 sample. However, pseudovirus assays showed approximately 50% inhibition at a 1:10 dilution in the day 13 sample (Supplementary Figure 1) . After the second dose of convalescent plasma on day 58, samples from days 65 and 71 showed ≥50% plaque reduction at a 1:16 dilution. Since IgG antibody against the S-protein RBD was not detected at any time point (days 4, 9, 13, 16, 45, 56, 71, and 72), the observed plaque reduction suggests the presence of low titers of neutralizing antibodies or other antibodies directed to non-RBD regions of the S-protein in the patient's plasma (Supplementary Table 2 ).

We report here sustained SARS-CoV-2 RNAemia with viral replication for >2 months and intrahost viral evolution in a patient with COVID-19 and both B-and T-cell depletion as a result of the therapies he had received for multiple myeloma. The initial clinical improvement and >10-fold reduction in plasma RNAemia (days 4-9) suggests a treatment response following convalescent plasma (day 2) and remdesivir (days 5-10). RNAemia was never completely suppressed, however, and we hypothesize that the absence of anti-SARS-CoV-2 humoral responses and the paucity of T-cell responses related to prior chemotherapy and anti-BCMA CAR T cells resulted in uncontrolled viral replication and overwhelming SARS-CoV-2 pneumonia (1.125 × 10 6 PFU/mL and 2.78 × 10 10 RNA copies/ mL in ETA) that likely contributed to death. The detection of a distinct SARS-CoV-2 sequence variant on day 13 of the first hospitalization with 3 coding mutations ( Figure 2 ) compared to the initial sequence variant detected on day 4 that matched the circulating GH clade argues for viral evolution within the host and against superinfection or reinfection. Several additional sequence variants, including those with coding mutations and deletions in the S gene that have yet to be detected in SARS-CoV-2 in Pittsburgh as of January 2021, were identified on day 67. Furthermore, additional sequence variants rapidly developed in the span of 5 days between days 67 and 72 while the patient was still in the hospital (Figure 2) , further supporting continued intrahost viral evolution, since reinfection with 5 distinct variants is highly improbable. Taken together, these findings provide insights into the potential duration of continued SARS-CoV-2 replication and the plasticity of the SARS-CoV-2 S gene, as others have noted [15] .

RNAemia has been described in immunocompromised patients with non-SARS-CoV-2 respiratory viruses. Respiratory syncytial virus (RSV) RNAemia developed in 30% of hematopoietic cell transplant recipients 2 days after onset of RSV pneumonia and was a predictor of mortality [16] . Emerging data suggest that SARS-CoV-2 RNAemia may be a marker of severity of COVID-19 pneumonia [5, 17] , but sustained RNAemia for >2 months has not yet been described. We were unable to isolate replication-competent virus from plasma, which may be due to technical issues, or may suggest that RNAemia is not caused by the presence of virions in the plasma, but rather due to "spillover" of infected cells with SARS-CoV-2 RNA from the lung. The reduction in plasma RNAemia between days 4 and 9 (after administration of convalescent plasma on day 2 and of remdesivir between days 5 and 10) suggests that viral replication was inhibited by the therapies given. Unfortunately, measurement of plasma RNAemia is not readily available for clinical use in the United States, and it is not currently known whether suppression of SARS-CoV-2 RNAemia can improve clinical outcomes. The rebound of viremia back to 101 800 RNA copies/ mL on day 71 occurred in the setting of the administration of dexamethasone. Whether corticosteroids should be avoided in heavily immunosuppressed individuals in whom prolonged SARS-CoV-2 replication is suspected warrants further study. Ultimately, randomized trials are needed to evaluate whether measurement of RNAemia should be used to determine clinical response to SARS-CoV-2 therapies.

We isolated virus from respiratory samples collected 72 days after COVID-19 onset, demonstrating the potential for infectivity late into the clinical course. In a prior study of immunocompetent patients with mild COVID-19, virus isolation from samples obtained after 8 days was unsuccessful [3] . Our findings suggest that certain severely immunosuppressed patients with COVID-19 may require isolation longer than the 20-day period currently proposed by the Centers for Disease Control and Prevention [18] . Whether PCR cycle thresholds (which were consistently <25 in our patient) can be used to make decisions related to discontinuation of transmission-based precautions warrants further investigation. Importantly, replication-competent variants isolated in cell culture harbored deletions associated with increased transmissibility similar to what has since been identified in the UK months later [12] . These findings raise the possibility that the origin of the highly mutated UK and South African variants may have been persons with protracted infection.

Humoral and cell-mediated immunity after COVID-19 appear to be necessary to control SARS-CoV-2 infection [19, 20] . In our patient, PBMC analysis confirmed B-cell aplasia, which is compatible with negative assay results for IgGs against the S-protein RBD, and IgAs/IgGs using a clinically approved assay. IgGs against the S-protein RBD were absent at all time points (days 4, 9, 13, 16, 45, 56, 71, and 72) despite the fact that the patient received convalescent plasma on days 2 and 58 of illness. This finding suggests either low titer of antibodies in the convalescent plasma or rapid clearance of antibodies in the context of high viral burden. Interestingly, there was evidence of low-level viral neutralization on days 65 and 71 (after the second dose of convalescent plasma) using live virus neutralization assays, which may suggest the presence of low titers of neutralizing antibodies directed to non-RBD regions of Spike or low titers of other antibodies in the convalescent plasma samples. The scRNA-seq profiling from days 4 and 9 showed upregulation of interferon-stimulated genes in monocytes, suggesting the presence of innate antiviral immunity, which was unable to control the infection. SARS-CoV-2-specific T-cell response assays did show some evidence of T-cell activation late into the disease course, although the T-cell lymphopenia related to antecedent chemotherapy limited our ability to draw conclusions about the patient's T-cell responses. Overall, these findings support the hypothesis that the diminished T-cell responses and nearly absent B-cell responses led to uncontrolled SARS-CoV-2 infection.

In conclusion, our case highlights that immunocompromised patients with profound lymphocyte defects such as CAR T-cell therapy recipients are at risk for prolonged SARS-CoV-2 replication. Other patients with severe lymphocyte deficiencies, such as anti-CD20 monoclonal antibody recipients, and hematopoietic cell or organ transplant recipients within the first few months of transplant, may also be at risk. Clinicians caring for such patients should be cautious not to attribute persistent detection of SARS-CoV-2 RNA from clinical samples of these patients to the presence of noninfectious virus and should consider revising their transmission-based precautions for these patients. Further work is needed to define the role of monitoring RNAemia in managing immunocompromised persons with COVID-19, the duration of infectivity in these patients, the importance of intrahost emergence of SARS-CoV-2 sequence variants in immune escape, and the immune phenotypes associated with recovery vs fatal infection. More effective antiviral agents to suppress SARS-CoV-2 replication are urgently needed, especially for immunodeficient hosts.

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Infections after anti-CD19 chimeric antigen receptor T-cell therapy for hematologic malignancies: timeline, prevention, and uncertainties

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Highly sensitive quantification of plasma SARS-CoV-2 RNA sheds light on its potential clinical value

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T cells expressing an anti-B-cell maturation antigen chimeric antigen receptor cause remissions of multiple myeloma

B-cell maturation antigen is a promising target for adoptive T-cell therapy of multiple myeloma

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is a scientific advisory board member for Tizona, Werewolf, and F-Star; is a consultant to Astellas, BMS, Almirall, and Incyte; and receives research funding from BMS and Novasenta. All other authors report no potential conflicts of interest.All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.

Supplementary materials are available at Clinical Infectious Diseases online. Consisting of data provided by the authors to benefit the reader, the posted materials are not copyedited and are the sole responsibility of the authors, so questions or comments should be addressed to the corresponding author.

Author contributions. M. K. H. and W. G. B. contributed equally to this work as co-first authors. J. J. and S. N. contributed equally to this work. All authors have seen and approved the manuscript and contributed significantly to this work.