key: cord-0750157-o95im4o0
authors: Shenoy, Anukul T.; Mizgerd, Joseph P.
title: Seedy CD8+ T(RM) cells in aging lungs drive susceptibility to pneumonia and sequelae
date: 2021-01-08
journal: Cell Mol Immunol
DOI: 10.1038/s41423-020-00629-w
sha: 10adac546c9f7b04610eacda5ebbbb2cda899c2d
doc_id: 750157
cord_uid: o95im4o0

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cells based on surface marker phenotype analysis and parabiosis experiments. Thus, after IAV infection, older lungs exhibit prolonged inflammation and excessive numbers of CD8 + T RM cells.

Excessive CD8 + T RM -cell accumulation is driven by old lungs rather than by old lymphocytes. To determine whether the excessive accumulation of CD8 + T RM cells in older lungs was due to aging lymphocytes or to T cell-extrinsic causes (such as aging lungs), Goplen et al. performed adoptive transfer experiments in which antigen-specific CD8 + T cells from young mice were transferred into younger or older host mice that were then infected with PR8 viruses expressing the relevant antigens. Even younger CD8 + T RM cells accumulated excessively in older lungs. Thus, the aging lung or some lymphocyte-extrinsic factors mediate this excessive T RM -cell accumulation. The older lungs expressed greater levels of TGF-β mRNA than the younger lungs after recovery from IAV infection, and CD8 + T cells without TGF-β receptor II signaling did not accumulate in older lungs. Altogether, these studies suggest that changes in older lung tissues, which include but may not be limited to overabundant TGF-β expression, cause an excessive accumulation of CD8 + T RM cells after the resolution of pneumonia.

CD8 + T RM cells in older lungs are not only numerous but also dysfunctional. Resident memory lymphocytes in the lung are important for protection against heterotypic infections. 1, 5 To test if the enhanced accumulation of CD8 + T RM cells in aged lungs confers protection against heterosubtypic IAV, Goplen et al. challenged older and younger mice with a lethal dose of the X31 strain of IAV 60 days after PR8 infection. While all the younger mice achieved immune protection due to PR8 exposure and survived X31 infection, most of the aged mice succumbed to the heterotypic infection despite the increased density of CD8 + T RM cells in their lungs. Single-cell RNA sequencing of the IAV-specific CD8 + T RM cells from the lungs of the older and younger mice revealed different clustering of cells at the different ages, and both the sequencing data and antigen stimulation assays suggested that aged CD8 + T RM cells were characterized by deficient IFN-γ production. In contrast to their aberrant accumulation, which was cell extrinsic, aberrant IFN-γ expression was cell intrinsic and reflected defects in the older lymphocytes rather than in the older lungs. Thus, aged lungs excessively accumulate CD8 + T RM cells that are intrinsically hyporesponsive to antigen challenge and ineffective at protecting against respiratory infection.

Chronic inflammation and fibrosis are products of CD8 + T RM cells in older lungs recovering from pneumonia. Although they are poor responders that do not protect against infection, aged CD8 + T RM cells are immunologically active in the lungs, promoting chronic pulmonary inflammation and fibrotic damage. The depletion of CD8 + cells at day 21 after PR8 infection was sufficient to diminish the chronic inflammation observed at day 60, including the percentage of the lung that was inflamed, the numbers of extravascular monocytes and neutrophils, and the transcripts for proinflammatory mediators. Collagen deposition was also reduced by CD8 + cell depletion, suggesting that the fibrosis in the aged lungs was driven by excessive and aberrant T RM cells. The cellular and molecular mechanisms by which CD8 + cells drive chronic inflammation and fibrosis remain undetermined and exciting areas for future investigation. Since CD4 + T RM cells can enhance acute pulmonary inflammation by posttranscriptional regulation of neutrophil-regulated chemokine genes in lung epithelial cells, 6 it will be interesting to see if CD8 + T RM cells exploit a similar type of signaling axis to enhance persistent inflammation in aged lungs.

Although this study contributes to the advancement of research, this study has limitations. The research was performed in mice, and human correlates have yet to be explored. Infections of mice with PR8 cause long-term tissue remodeling that includes a degree of lymphoid aggregates and epithelial anomalies that may not be typical of respiratory infections with influenza viruses or other microbes, 3,4 so extrapolating to other respiratory pathogens requires caution. Goplen et al. studied older mice that were naive to IAV, which does not account for the naturally acquired heterotypic immune memory that typifies adult mammals in the wild. Microbial histories change systemic and tissue immunity and make the immune responses of laboratory mice more closely resemble those of adult humans. 1, [7] [8] [9] The lack of infectious disease and microbiome histories in these mouse studies may influence the excessive and aberrant T RM -cell responses in the aged lungs that were observed here. Despite these limitations, the studies by Goplen et al. are ground-breaking due to their compelling discovery that aging lungs recruit excessive numbers of CD8 + T RM cells, that aging CD8 + T RM cells are ineffective for preventing heterotypic infection, and that lung CD8 + T RM cells promote chronic inflammation and pulmonary fibrosis after recovery from influenza infection.

Pneumonia is a key feature of aging, since older people are more susceptible to pneumonia and older people suffer more greatly from pneumonia. 1 As~20% of the global population approaches ≥65 years of age, 10 understanding the disorders of pulmonary immunity that are causes or consequences of pneumonia has become increasingly necessary. The COVID-19 pandemic and its pronounced impact on the elderly population further emphasize these needs. The study by Goplen et al. highlights a specific immunological defect in aging lungs that both increases susceptibility to pneumonia and exacerbates downstream pneumonia sequelae. Future studies should further investigate when and how CD8 + T RM cells accumulate excessively or behave inappropriately. Measures or correlates of CD8 + T RM -cell function in humans have become an important goal and might be useful for identifying individuals with increased susceptibility to pneumonia or with a greater propensity for chronic inflammatory responses, fibrotic sequalae, or other long-term outcomes after pneumonia. For those in whom deleterious CD8 + T RM -cell activities mediate long-term sequelae of pneumonia, inhibiting CD8 + T-cell activities may be an avenue worth exploring for therapeutic approaches. Insights into age-associated dysfunctions in lung immunity against infection, such as those from Goplen et al., are helpful for developing precision medicine strategies for preventing and treating pneumonia and its disastrous consequences in this most relevant population of pneumoniasusceptible subjects.

Seedy CD8+ T RM cells in aging lungs drive

The authors of this work are supported by NIH grants including R01-AI115053, R35-HL135756, and R33-HL137081 to J.P.M.

Both authors wrote this manuscript together and approved the manuscript. 

Competing interests: The authors declare no competing interests.