key: cord-0748867-vkeqx8ku authors: da Rosa, Taciéli Fagundes; Foletto, Vitória Segabinazzi; Serafin, Marissa Bolson; Bottega, Angelita; Hörner, Rosmari title: Anti-infective properties of proton pump inhibitors: perspectives date: 2021-09-03 journal: Int Microbiol DOI: 10.1007/s10123-021-00203-y sha: 62296805aafbd3df2b78b1bc8767ff1b9318b430 doc_id: 748867 cord_uid: vkeqx8ku Infectious diseases are among the main causes of morbidity and mortality today. In facing this crisis, the development of new drug options and combat strategies is necessary. In this sense, drug repositioning or drug redirection has emerged for the faster identification of effective drugs. In this “Commentary,” the anti-infective properties of the class of proton pump inhibitors (PPIs) are emphasized. Studies report activities against bacterial, fungal, parasitic, and viral agents. In addition, we have provided in a table a summary of the specific characteristics of PPIs and some of their anti-infective activities. Infectious diseases are among the main causes of morbidity and mortality today. This problem is compounded by the current crisis of resistance to antibacterial, antifungal, antiparasitic, and antiviral drugs, which has become a public health issue not only in terms of limited treatment options but also because of its economic burden. In facing this crisis, studies that analyze the causes of resistance and its expansion, together with the development of new drug options and combat strategies, are necessary (Nolte 2014; Gil-Gil et al. 2019; Nathan 2020) . A promising alternative is drug repositioning. Repositioning, or also called redirection or repurposing, has been a trending topic in the literature and represents a new drug development strategy. This method consists of finding new uses for clinically approved drugs that already have a known chemical structure, toxicity, and safety profile. Thus, they can be redirected in the treatment of emerging diseases and pandemics, due to their speed of implementation, effectiveness, and lower costs when compared to the development of a new drug (Ashburn and Thor 2004; Serafin and Hörner 2018; Peyclit et al. 2019; Zhou et al. 2020) . Studies reporting the importance of drug repositioning for the treatment of infectious diseases are available, and among them we cite several classes, such as antidepressants da Rosa et al. , 2021 Foletto et al. 2020 Foletto et al. , 2021 Machado et al. 2020; Serafin et al. 2020) , antihypertensive (Hu et al. 2018) , antihistamines (Bruer et al. 2019; El-Nakeeb et al. 2011 ), anti-inflammatory (Chan et al. 2017 , alcohol-aversive agentes ), benzodiazepines (da Rosa et al. 2021 , and statins (Rampelotto et al. 2018) . In this commentary, the class of proton pump inhibitors (PPIs) is emphasized. PPIs are known for their use in stomach acid-related disorders. Your representatives-dexlansoprazole, esomeprazole, lansoprazole, omeprazole, pantoprazole, rabeprazole, and tenatoprazole-are derived from the heterocyclic organic molecule benzimidazole and are first-line agents for the treatment of non-erosive reflux disease, peptic ulcer, Zollinger-Ellison syndrome, prevention of ulcers induced by non-steroidal anti-inflammatory drugs, eosinophilic esophagitis in pediatric patients, and eradication of Helicobacter pylori when in combination with antibacterials (Strand et al. 2017; Ward and Kearns 2013) . These drugs were clinically introduced over 25 years ago and have since proven to be invaluable, safe, and effective agents for the treatment of a variety of gastric acid-related disorders. Although adverse effects related to the use of PPIs have been reported, their clinical relevance is still unclear, as the evidence reported in these studies is based on retrospective observational studies. When compared to previous agents, this class demonstrates consistent patient tolerance, excellent safety, and generally superior acid-suppressing capacity. These are drugs widely used by the population, considering that omeprazole is among the 10 most prescribed in the USA (Perry et al. 2020; Strand et al. 2017) . PPIs have been continuously studied for presenting other activities, in addition to those already known and used commercially, and the most reported in the literature are the anti-infective ones. These properties are reported against different infectious agents: antibacterial activity, against Pseudomonas aeruginosa (Sadiq et al. 2020) , Mycobacterium tuberculosis (Mdanda et al. 2017) , Ureaplasma urealyticum (Nagata et al. 1995) , Enterococcus faecalis (Jonkers et al. 1996) , and Staphylococcus aureus (Jonkers et al. 1996; Vidaillac et al. 2007 ); antifungal, against Candida albicans (Biswas et al. 2001; Monk et al. 1995) , Candida spp. (Siavoshi et al. 2012) , and Saccharomyces cerevisiae (Monk et al. 1995 (Riel et al. 2002; Skinner-Adams et al. 1997) , Schistosoma mansoni (Almeida et al. 2015; Ellakany et al. 2019) , Giardia lamblia (Hernández-Ochoa et al. 2017) , and Tritrichomonas foetus (Sutak et al. 2004; Kather et al. 2007 ); antiviral, against SARS-CoV-2 (COVID-19) (Aguila and Cua 2020; Homolak and Kodvani 2020) and rhinovirus (Sasaki et al. 2005) . In Table 1 , we present a summary of the characteristics of PPIs and some of their anti-infective activities. The activity of PPIs against COVID-19 is controversially reported. Some studies report that omeprazole would act positively against the virus, as previous studies had already reported that omeprazole was able to inhibit viral replication by interfering with the acidification of lysosomes. Drugs that affect the activity of vesicular acidification mechanisms neutralize endolysosomal compartments. A PPI can neutralize these compartments through inhibition of vacuolar-type H + -ATPase (V-ATPase). Furthermore, it is still presented that the class of PPIs could help not only in the treatment, but also in the prophylaxis against the virus (Aguila and Cua 2020; Homolak and Kodvani 2020; Shen et al. 2017; Tastemur and Ataseven 2020; Vuille-dit-Bille et al. 2015) . On the other hand, other studies report that patients taking PPIs are at increased risk for serious clinical outcomes of COVID-19. It was found that individuals who take drugs in this class twice a day are more likely to report a positive test for SARS-CoV-2, when compared to those who use a lower dose up to once a day. The mechanism of this worrisome clinical outcome would occur because PPIs would alter one of the main functions of the gastric juice, which is to inactivate ingested microorganisms, thus inhibiting infectious agents from reaching the intestine (Almario, Chey and Spiegel 2020; Charpiat et al. 2020; Lee et al. 2021) . These considerations about the activities of PPIs against COVID-19 lead us to question what are the perspectives for the future use of PPIs as anti-infective agents? Several drug classes that present significant adverse effects and control by medical prescription are used in studies with the objective of verifying the anti-infective activities. PPIs have a great advantage to be considered, since they are drugs that do not require a medical prescription to be marketed, have an excellent safety profile and ease of administration, and are among the most used drugs worldwide (Scarpignato et al. 2016 ). More studies are always welcome in clinical research to confirm the anti-infective potential of this class, but we can say that the therapeutic advantage will be great. Therapeutic activities are significantly reported in the literature, demonstrating the interest of researchers in this topic. Thus, the future perspectives for the use of anti-infective activities of PPIs are the best. 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