key: cord-0745613-dokvxhqm
authors: Rabalski, Lukasz; Kosinski, Maciej; Mazur-Panasiuk, Natalia; Szewczyk, Boguslaw; Bienkowska-Szewczyk, Krystyna; Kant, Ravi; Sironen, Tarja; Pyrć, Krysztof; Grzybek, Maciej
title: Zoonotic spillover of SARS-CoV-2: mink-adapted virus in humans
date: 2021-03-05
journal: bioRxiv
DOI: 10.1101/2021.03.05.433713
sha: 86fbd89eb3f7240f53f575749f76ff3b96adeba5
doc_id: 745613
cord_uid: dokvxhqm

The COVID-19 pandemic caused by SARS-CoV-2 started in fall 2019. A range of different mammalian species, including farmed mink, have been confirmed as susceptible to infection with this virus. We report here the spillover of mink-adapted SARS-CoV-2 from farmed mink to humans after extensive adaptation that lasted at least 3 months. We found the presence of four mutations in the S gene (that gave rise to variant: G75V, M177T, Y453F and C1247F) and others in an isolate obtained from SARS-CoV-2 positive patient.

Coronaviruses are known as potential zoonotic pathogens, and severe acute respiratory 

The capacity of coronaviruses to transfer between mammalian species is not surprising. First, 74 the transfer of highly pathogenic variants from bats usually occurs via intermediate hosts.

Infection of dromedary camels and palm civets and later transfer to humans has been 76 described for MERS-CoV and SARS-CoV, respectively (9) . A more comprehensive analysis 77 of existing strains suggests that this was not an exception. The human coronavirus OC43 p  o  s  s  i  t  i  o  n  a  a  v  a  r  i  a  n  t  R  e  f  e  r  e  n  c  e  G  r  o  u  p  A  G  r  o  u  p  B  G  r  o  u  p  C   1  2  1  8  n  s  p  2  :  S  1  3  8  L  C  T  T  T  1  6  3  0  n  s  p  2  :  Q  2  7  5  H  A  T  T  T  2  4  2  6  n  s  p  2  :  V  5  4  1  I  G  A  A  A  3  3  7  3  n  s  p  3  :  D  2  1  8  E  C  A  A  A  5  3  2  6  n  s  p  3  :  E  8  6  9  D  G  T  T  T G  T  T  G  2  9  3  0  0  N  :  D  3  4  3  Y  G  T  T  G 

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The complete genome sequences of SARS-CoV-2 isolated from the patient was deposited in 189 GISAID under the accession number

Authors' contributions

The study was conceived and designed by LR and MG. Data handling: LR

Statistical and phylogenetic analysis 193 was carried by LR. Interpretation of data: LR, KP, MK. Data visualisation: LR

KBS in consultation 195 with all co-authors. MG, KP, LR, TS, RK revised the manuscript. All authors accepted the 196 final manuscript version

We gratefully acknowledge the Authors from the Originating laboratories responsible for 199 obtaining the specimens and the Submitting laboratories where genetic sequence data were 200 generated and shared via the GISAID Initiative