key: cord-0745095-zvkapzpv authors: Peiris, Malik; Cheng, Samuel; Mok, Chris Ka Pun; Leung, Yonna; Ng, Susanna; Chan, Karl; Ko, Fanny; Yiu, Karen; Lam, Bosco; Lau, Eric; Chan, Ken; Luk, Leo; Li, John; Tsang, Leo; Poon, Leo; Chen, Chunke; Hui, David title: Neutralizing antibody titres to SARS-CoV-2 Omicron variant and wild-type virus in those with past infection or vaccinated or boosted with mRNA BNT162b2 or inactivated CoronaVac vaccines date: 2022-01-05 journal: Res Sq DOI: 10.21203/rs.3.rs-1207071/v1 sha: 8c1cd3c560bdef25e654ad844d2ea1ada6a847a0 doc_id: 745095 cord_uid: zvkapzpv Omicron, a novel SARS-CoV-2 variant has emerged and is rapidly becoming the dominant SARS-CoV-2 virus circulating globally. It is important to define reductions in virus neutralizing activity in serum of convalescent or vaccinated individuals to understand potential loss of protection from infection or re-infection. Two doses of BNT162b2 or CoronaVac vaccines provided little 50% plaque reduction neutralization test (PRNT(50)) antibody immunity against the Omicron variant, even at one-month post vaccination. Booster doses with BNT162b2 in those with two doses of either BNT162b2 or CoronaVac provided acceptable neutralizing immunity against Omicron variant at 1-month post-booster dose. However, three doses of BNT162b2 elicited higher levels of PRNT(50) antibody to Omicron variant suggesting longer duration of protection. Convalescent from SARS-CoV-2 infection did not have protective PRNT(50) antibody levels to Omicron, but a single dose of BNT162b2 vaccine provided protective immunity. Field vaccine-efficacy studies against Omicron variant against different vaccines are urgently needed. Omicron, a novel SARS-CoV-2 variant has emerged and is rapidly becoming the dominant SARS-CoV-2 virus circulating globally. It is important to de ne reductions in virus neutralizing activity in serum of convalescent or vaccinated individuals to understand potential loss of protection from infection or re-infection. Two doses of BNT162b2 or CoronaVac vaccines provided little 50% plaque reduction neutralization test (PRNT 50 ) antibody immunity against the Omicron variant, even at one-month post vaccination. Booster doses with BNT162b2 in those with two doses of either BNT162b2 or CoronaVac provided acceptable neutralizing immunity against Omicron variant at 1-month post-booster dose. However, three doses of BNT162b2 elicited higher levels of PRNT 50 antibody to Omicron variant suggesting longer duration of protection. Convalescent from SARS-CoV-2 infection did not have protective PRNT 50 antibody levels to Omicron, but a single dose of BNT162b2 vaccine provided protective immunity. Field vaccine-e cacy studies against Omicron variant against different vaccines are urgently needed. Text A novel SARS-CoV-2 variant with increased transmissibility was rst reported in South Africa in November 2021 1 , classi ed as a variant of concern and named Omicron 2 . The virus had 37 amino acid substitutions in the spike protein of the virus, 15 of them being in the receptor binding domain. It was predicted that some of these amino acid substitutions would have major impact in evading neutralizing antibody. Virus neutralizing antibody is a major determinant of protection from infection in humans and in macaques experimentally challenged with virus 3, 4 . Neutralizing antibody thresholds associated with protection from re-infection or severe disease have been reported 5, 6 . In experimentally infected macaques convalescent from SARS-CoV-2 infection, depletion of CD8 T cells reduced the protective effect from re-infection, suggesting that T cell immunity can contribute to protection 4 . However, quantitative correlates of T cell protection remain elusive. Studies on the immunogenicity and vaccine e cacy of BNT162b2 vaccine have been reported, but whole virus inactivated vaccines such as CoronaVac (Sinovac) are less studied. CoronaVac is one of the WHO approved vaccines and over 750 million doses have been administered in more than 40 countries. Phase-three randomized clinical trials of CoronaVac showed e cacies ranging from 50.7% and higher protection from severe disease 7, 8 . But there have been reports of breakthrough infections leading to severe disease and death in CoronaVac vaccinated adults 9 . Data on the immunogenicity of current COVID-19 vaccines against Omicron variant, is urgently needed. We had previously demonstrated that those vaccinated with BNT162b2 had markedly higher levels of geometric mean PRNT 50 antibody titres compared to those vaccinated with CoronaVac vaccines, at one-month post-second dose of vaccine 10 . Allowing for antibody waning, we estimated that only 16% of the CoronaVac vaccinated individuals would retain protective thresholds against the wild-type virus while 79.6% of BNT162b2 vaccinees would be similarly protected by six months after second dose of vaccine 10 . Subsequently, we randomized the cohort receiving CoronaVac vaccine to receive booster doses of CoronaVac or BNT162b2 and showed marked increase in neutralizing antibody following boosting with BNT162b2 but a less optimal responses with CoronaVac 11 . However, the emergence of virus variants that are able to partially evade vaccine immunity would compromise vaccine protection. It is important that we rapidly compare virus neutralizing antibody levels to the wild-type virus to which vaccines are developed and Omicron variant, in persons who have received vaccinations or booster doses, for formulation of appropriate public health policy. Here we compare PRNT antibody titres to wild-type SARS-CoV-2 and Omicron variant in previously studied cohorts who recovered from COVID-19 12 , uninfected community subjects one month after receiving two doses of BNT162b2 or CoronaVac vaccine 10 , or one month after receiving booster doses of the respective BNT162b2 or CoronaVac or those who received two doses of CoronaVac and received a booster dose of BNT162b2 vaccine 11 . COVID-19 recovered individuals receiving one dose of vaccines were also studied. Methods used for sampling of participants, serological tests and statistical analyses are detailed in Supplementary PRNT 90 data is shown in Supplementary gure 2. PRNT 50 titres in COVID-19 vaccinated or convalescent cohorts were markedly lower to Omicron variant than to wild-type virus ( Figure 1A-C, Table1) . The fold reduction in comparative GMT to Omicron vs wild-type was 30.9-fold for those receiving two doses of BNT162b2 vaccine, 10.5-fold in COVID-19 convalescent sera and 6.4-fold in those receiving two doses of CoronaVac vaccine ( Figure 1A, Table1) . However, comparison of fold-difference in GMT to the two viruses is confounded by groups with low GMT to the wild-type virus because a smaller fold-reduction would already reach the detection threshold. Others report comparable reductions in neutralizing titres to Omicron variant neutralizing titres in sera of vaccinated or COVID-19 convalescent individuals 13, 14 . Using methods previously described by Khoury and colleagues 5 , we had previously determined that the threshold for 50% protection from symptomatic SARS-CoV-2 infection was a PRNT 50 titre of 25.6 with 95% con dence intervals of 1:18.3-1:36.0 12 Three returning travelers diagnosed with Omicron variant were studied as controls. They were males (range in age from 36-62) vaccinated with two doses of RNA (BNT162b2 or mRNA-1273) vaccines (see Supplementary information for details). Cases 12388 and 12432 had detectable PRNT 50 antibody (titres of 80 and 160, respectively) to wild-type virus on the day of rst RT-PCR detection or the day after, but did not have detectable cross-neutralizing antibody to Omicron variant ( Figure 1D ), explaining why they had breakthrough infections. They rapidly mounted neutralizing antibody to Omicron variant of ≥1:320 by day 4 (Case 12432) or day 13 (sera between day 1 and day 13 not being available for case 12388). Serum from Case 12404 was rst available at days 7 by which time he had PRNT 50 titres of ≥1:320 to both wild-type and Omicron variant. This rapid neutralizing antibody response in RNA vaccinated individuals to an antigenically divergent variant suggests that such individuals are likely to be protected from severe disease. We included the WHO standard NIBSC 20/136 in two PRNT esperiments with PRNT 50 titres of 1:20 and 1:40 respectively with Omicron and 1:320 with wild-type virus. This study had some limitations. There were relatively small numbers in the individual groups, but as estimated from our power calculations (see Supplementary information), the sample size was adequate to demonstrate signi cant conclusions. We have not investigated other antibody activities such as binding to spike protein or binding to Fc receptors which may confer antibody-dependent cell cytotoxicity via natural killer cell mediated (ADCC). In a previous study with this cohort, we showed that both spike binding antibody and FcgRIIIa binding antibodies paralleled the PRNT antibody responses with these two vaccines 10 . However, it is possible that these antibodies may be less affected by the mutations found in the Omicron variant and may play relatively more important protective roles in protection from Omicron variant. We have not investigated T cell responses elicited by these vaccines that may provide protection, at least against severe disease. In summary, we demonstrate that two doses of BNT162b2 or CoronaVac vaccines provide little neutralizing antibody immunity against the Omicron variant, even at one-month post vaccination. Booster doses with BNT162b2 in those with two doses of either BNT162b2 or CoronaVac provide acceptable neutralizing immunity against Omicron variant at 1-month post-booster dose. However, because three doses of BNT162b2 elicit higher levels of PRNT 50 antibody to Omicron variant than two doses of CoronaVac followed by a booster with BNT162b2, waning antibody is likely to result in faster loss of protection with the latter schedule. It must be noted however, that CoronaVac, a whole virus inactivated vaccine with multiple viral proteins, has previously been shown to elicit a wider range to T cell responses which may compensate for some loss of neutralizing antibody protection 10 Respirology Epub ahead of print Clin Infect Dis (2021) Epub ahead of print Table Table: Age, sex and geometric mean 50% plaque reduction neutralization test (PRNT 50 ) titres vs