key: cord-0742941-i3g8452v
authors: Dominic C.D., Midhun; Joseph, Rani; Sabura Begum, P.M.; Raghunandanan, Aswathy; Vackkachan, Nelwin T.; Padmanabhan, Dileep; Formela, Krzysztof
title: Chitin nanowhiskers from shrimp shell waste as green filler in acrylonitrile-butadiene rubber: Processing and performance properties
date: 2020-06-02
journal: Carbohydr Polym
DOI: 10.1016/j.carbpol.2020.116505
sha: 272b2de541252979f74ef065f1301fadfdbb57a8
doc_id: 742941
cord_uid: i3g8452v

In this work, chitin nanowhiskers with high crystallinity index were obtained from shrimp shells waste using acid hydrolysis method and then comprehensively characterized. Subsequently, the impact of chitin nanowhisker content on processing and performance of acrylonitrile-butadiene rubber based nanocomposites was evaluated. The results showed that the addition of chitin nanowhiskers increased tensile strength and tear strength of nanocomposites by 116% and 54%, which was related to suitable dispersion of chitin nanowhiskers in matrix. Reinforcing effect of chitin nanowhiskers in acrylonitrile-butadiene rubber was also confirmed by Wolff activity coefficient, glass transition temperature and equilibrium swelling measurements. Moreover, it was found that higher content chitin nanowhiskers significantly improve the thermal stability of studied nanocomposites. The incorporation of chitin nanowhiskers resulted in increase of 74 °C for onset degradation temperature. This work confirmed that shrimp shell waste can be upcycled into chitin nanowhiskers - promising green filler in NBR for high-performance elastomeric applications.

has excellent resistance to oil, chemicals, radiation and gas permeability and the properties of NBR can be easily modified by different acrylonitrile content (Fazli & Rodrigue, 2020) . Even though NBR has excellent oil resistance its mechanical strength without the reinforcement phase is weak, because it does not crystallize under stress (Sadeghalvaad, Dabiri, Zahmatkesh, & Afsharimoghadam, 2019) . For high-performance elastomeric applications of NBR, its mechanical strength should be improved. Reinforcing NBR with different nanofillers is a suitable method to improve its performance properties.

It was reported that the addition of unmodified montmorillonite (Na-MMT) nanoclay into NBR significantly improve its modulus, tensile strength, thermal stability and glass transition temperature (Kader, Kim, Lee, & Nah, 2006) . The dispersion of nano-CaCO3 in NBR can be improved by the addition of the organoclay (Cloisite 30B) (Salkhord & Sadeghi Ghari, 2015) .

They found that the use of hybrid filler based on nano-CaCO3 (10 phr) and Cloisite 30B (6 phr) provide a tremendous improvement in the dynamic mechanical, tensile and transport properties of NBR. Hu's group investigated the use of graphene oxide/halloysite nanotubes as hybrid fillers in NBR (Hu et al., 2020) . They reported significant improvement of cross-link density, rubbery storage modulus and glass transition temperature of NBR based nanocomposites. The glass transition temperature of neat NBR increased from -19 C to -11.5 C by introducing 1 phr graphene oxide hybridized with nanosilica which shows the effectiveness of this hybrid filler system(Z. Zhang, He, Wang, Rodrigues, & Zhang, 2018) . Nanosilica was found to be a good reinforcing filler in NBR because of the hydrogen bonding interaction between the surface silanol groups of nanosilica and the nitrile group of NBR (Eyssa, Abulyazied, Abdulrahman, & Youssef, 2018) .

After a thorough investigation of the literature, it was possible to observe that NBR rubber is mostly reinforced with fillers derived from non-renewable sources. Utilizing bio/green fillers to improve the overall properties of NBR is hardly to find. Maria's group investigated the reinforcing effect of rice husk silica in NBR (Maria et al., 2017) . They reported that the addition of rice husk silica in NBR improves the Payne effect, thermal stability, and compound viscosity considerably. Li et. al reported that the anti-skid properties and thermal stability of NBR were improved by the addition of biochar obtained from rice bran (Li, Zhang, & Cho, 2014) . Abdul et.

al reported that the addition of 2 phr nanocrystalline cellulose in NBR, improved the thermal stability and activation energy by 75%, storage modulus by 12 GPa compared to virgin NBR J o u r n a l P r e -p r o o f (Abdul Rashid, Muhd Julkapli, & Abdul Hadi Yehya, 2018) . Cotton linter derived cellulose nanocrystals in NBR showed excellent improvement in the tensile strength, modulus, and elongation at break at an optimum loading of 5 phr without altering the tear strength (Azman Mohammad Taib, Yeheye, & Muhd Julkapli, 2020) Strong dependence on renewable resources has arisen as petroleum resources are limited. It has eventually led to the discovery and development of bio-based next-generation materials like nanocellulose and chitin nanowhiskers. Chitin, the second most abundant biopolymer is a polysaccharide with the molecular formula (C8H13O5N)n. It is a long-chain polymer of Nacetylglucosamine. Chitin is abundantly found in the exoskeleton of crab, mollusks, lobster, crayfish, shrimps, and krill etc.(X. Zhang & Rolandi, 2017) . Recently, chitin was extracted from the guano of the bat Rhinolophus hipposideros (Kaya, Seyyar, Baran, & Turkes, 2014) . Chitin was also extracted from fungi (Ekblad Alf, 1996) and corals (Bo et al., 2012) . Based on the difference in the alignments of N-acetylglucosamine chains, they are classified into α (antiparallel chains with strong intermolecular interactions), β (parallel chains with weak intermolecular interactions) and γ ( two chains going in the same direction and the other chain is antiparallel to them) (Alvarez, 2014) . Chitin nanowhiskers have been emerging as a sustainable alternative for synthetic or petrochemical-based materials because of its unique properties like high modulus, stiffness, aspect ratio, strength, non-toxicity, anti-microbial action, biodegradability, crystallinity, biocompatibility, high binding energy and liquid-crystalline behaviour (Nge, Hori, Takemura, Ono, & Kimura, 2003) . Due to these unique properties, chitin finds application in various fields such as food industry, the pharmaceutical industry, textile industry, paper industry, biomedical industry, cement industry, biosensing, drug delivery, etc. (Mincea, Negrulescu, & Ostafe, 2012) . Chitin nanowhiskers can be prepared by acid hydrolysis (Lu, Weng, & Zhang, 2004 ), ultrasonication(Villa-Lerma et al., 2013 , 2,2,6,6tetramethylpiperidine-1-oxyl radical mediated oxidation (Fan, Saito, & Isogai, 2008) , electrospinning method (Pillai & Sharma, 2009) , mechanical treatment (Aklog et al., 2016) , using ionic liquid (Kadokawa, Takegawa, Mine, & Prasad, 2011) , fermentation process (Sini, Santhosh, rubber(Liu, Liu, Yang, Luo, & Zhou, 2018) . They found that chitin nanocrystals forms a highly ordered network in natural rubber because of their capability to form inter and intra-molecular hydrogen bonding. The authors also found that significant improvement in the mechanical properties and thermal stability was obtained by adding 10 wt% of chitin nanocrystals in natural rubber latex. A study conducted by Gopalan et. al revealed that chitin nanowhiskers derived from crab shells can form strong three dimensional networks in natural rubber latex, thereby improving overall mechanical properties of the resulting composite (Gopalan Nair & Dufresne, 2003) . It was reported that β-chitin derived from squid pens, reinforced soy protein films show excellent tensile properties and water uptake resistance (Garrido, Etxabide, de la Caba, & Guerrero, 2017) . Visakh et. al reported that the addition of crab shell chitin significantly increases the cure rate index and barrier properties of carboxylated styrene butadiene rubber (XSBR) (Visakh, Monti, et al., 2012) .

India especially the city Kochi ('Queen of Arabian Sea') is famous for its seafood export. It is estimated that the shellfish industry in India generates 60000-80000 tonnes of waste annually (Ravi Kumar, 2000) . The shell waste produced in the pre-processing industry is a great threat because it creates disposal problems, pollution and health hazards (Biradar, Maladkar, & Kittur, 2016) . Producing chitin from these shell waste is an effective strategy to overcome these problems. The foremost reason for choosing chitin is its abundant availability in these shell wastes and it remains one of the most underutilized organic polymers in nature's recipe book.

In the present study, we propose the preparation of chitin nanowhiskers (CHNW) from shrimp shell waste by the acid hydrolysis method. The prepared CHNW was comprehensively characterized using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and thermogravimetric analysis (TGA). Subsequently, novel nanocomposites based on acrylonitrile-butadiene rubber reinforced by various content of CHNW were developed and their cure characteristics, physico-mechanical, thermal, dynamic mechanical and transport properties were investigated. As far as we know, no attempts have been put forward so far to evaluate the reinforcing efficiency of chitin nanowhiskers in NBR, therefore the proposed study has several advantages. Preparation of chitin nanowhiskers from shrimp shell waste facilitates 'Blue Growth' (Boonstra, Valman, & Björkvik, 2018) in India which consists of many seafood exporting companies. This is a practical method to reduce the waste disposal and pollution problem associated with seafood exporting industries. The isolation J o u r n a l P r e -p r o o f of the value-added product chitin nanowhiskers from shrimp shell waste will generate additional revenue to seafood industries, which suffers a decline in their export due to current Corona virus COVID-19 pandemic.The present work is a humble attempt to ensure environmental sustainability and to improve the environmental footprint in this highly industrialized global society.

Shrimp shells were collected from the local market in Kochi, Kerala, India. 

The shrimp shells were washed thoroughly with water several times and the washed shells were dried in an oven at 100 C for 24h.The dried shells were ground to a fine powder. About 100 g of the shrimp shell powder was refluxed with 2N oxalic acid (1:10 g/mL) for 4h. The acid is completely removed and the demineralised shrimp shell powder was subjected to bleaching.

About 100 g the dried powder was stirred with 1000 mL 15% hydrogen peroxide (v/v) and 100 mL 10% NaOH solution. The bleaching process was repeated 2-3 times until the powder became white. After the bleaching, the chitin microfibers were thoroughly washed with distilled water.

The obtained chitin fibers were refluxed with 3N HCl (1:10 g/mL) for 2h.The suspension was centrifuged and dialyzed in running water until the pH became 6. The suspension was subjected J o u r n a l P r e -p r o o f to homogenization in a D LAB homogenizer model D-500 for 30 minutes to get chitin nanowhisker.

NBR-CHNW composites were prepared in a two-step process. First step involves the masterbatch preparation in NBR latex followed by compounding the masterbatch with solid NBR and vulcanizing agents using two-roll mills. A good dispersion of CHNW in NBR latex (37% dry rubber content) was obtained by mixing the suitable amount of CHNW in NBR latex for 3 hours. The dispersion is coagulated using 0.5% CaCl2 solution. The obtained sheet was oven dried at 70 °C for 48h. CHNW loadings were adjusted to get 2, 4, 6 phr nanowhisker in the final composites. In the second step, the compounding of the masterbatch with solid NBR and other vulcanizing agents was carried out using two-roll mill. Chitin nanowhiskers were oriented in the mill direction by passing through the tight gap in the mill at the end of the mixing process.

The formulation for preparing NBR-CHNW composites is given in Table 1 . Vulcanization of various test samples was carried out in an electrically heated hydraulic press having 45cm × 45cm platen at 160 °C at a pressure of 2844 psi on the mould up to optimum cure time.

Cured NBR-CHNW composites were conditioned for 24 hours. Swelling behavior and cross-link density of NBR-CHNW nanocomposites were determined by equilibrium swelling method (Dominic C.D. et al., 2020) and the solvent used was toluene. Mol % uptake (Qt) of solvent for the samples was calculated using equation (1):

Where Ws is the weight of the swollen sample and Wi the initial weight of the sample i.e. the weight of the sample before swelling, Ms the molar mass of solvent.

Swelling index was calculated using the following equation (2):

Where Ws is the weight of the swollen sample and Wi the initial weight of the sample i.e.

the weight of the sample before swelling.

Cross-link density (ν) was calculated according to equation (3):

Where Mc is the molecular mass between successive cross-links. Mc is given by the Flory-Rehner equation (4) (Flory & Rehner, 1943) :

Where ρr is the density of rubber, Vs the molar volume of the solvent, χ the rubber solvent interaction parameter and Vr the volume fraction of swollen rubber which was calculated using Ellis and Welding equation (5) (Bryan & G. N. Welding, 1964) :

Where d is the deswollen weight of the polymer, f the volume fraction of insoluble components, w is the initial weight of the polymer, Asthe amount of solvent absorbed and ρs density of the solvent. χ is determined using Hildebrand equation (6):

Where is the lattice constant, R the universal gas constant, T the absolute temperature, δs and δp are solubility parameters of the solvent and polymer respectively.

TA Instruments DMA Q800 Dynamic mechanical analyser was used to measure dynamic mechanical properties of the samples in tension mode. Samples for DMA measurement were cut from the vulcanized sheets, having the dimension 30 mm x 3 mm x 2 mm. For the temperature sweep analysis, the samples were tested from -40 to 80 °C at a ramp rate of 3 °C/min and a frequency of 1 Hz.

The amount of polymer chains immobilized through matrix-filler interactions is determined by using equation (7) (Formela et al., 2016) :

Where is the volume fraction of the immobilized polymer chains,C 0 stands for the volume fraction of the immobilized polymer chains in the pure acrylonitrile-butadiene rubber

(8)

J o u r n a l P r e -p r o o f (taken to be zero), and W and W 0 are energy loss fraction for NBR-CHNW composite and pure NBR, respectively. Energy loss fractions can be calculated from the tan δ in accordance to equation (8):

= π tan δ π tan δ + 1

For better understanding the interfacial interactions between NBR and CHNW, the adhesion factor ( ) was calculated from the loss factor (tan δ) using equation (9) (Formela et al., 2016) :

Where, is the volume fraction of the filler phase, tan δc the loss tangent of NBR-CHNW composite and tan δm the loss tangent of pure NBR.

The inter and intra-molecular hydrogen bonding interaction in chitin nanowhiskers is shown in Fig. 1 . The FTIR spectroscopy was found to be very useful to distinguish the three crystalline polymorphs of CHNW (α, β, and γ) and its molecular interactions. The FTIR spectrum of the obtained chitin nanowhiskers was shown in Fig. 2A . Generally, chitin shows absorption bands around 1660, 1550, 973 cm -1 which are labelled as amide I, II and III bands (Jang, Kong, Jeong, Lee, & Nah, 2004) . Literature shows that for α-chitin, two separate absorbance bands are observed at 1656 and 1622 cm -1 (Jang et al., 2004) . The band at 1656 cm -1 , corresponds to the intermolecular hydrogen bonding (-CO...HN) and the band at 1622 cm -1 indicates the intramolecular hydrogen bonding (-CO…HOCH2) (Jang et al., 2004) . For β-chitin, only one absorption band is observed at 1659 cm -1 which corresponds to the stretching of -CO group (amide I) hydrogen-bonded to amide II (-NH-).The FTIR spectral analysis of the prepared CHNW showed that the amide I absorption band splits into two, and the bands were observed at 1656 and 1622 cm -1 . This reveals that α-form is present in the prepared CHNW. The bands at 3457 cm -1 and 3261 cm -1 correspond to the -OH stretching and -NH stretching respectively. The band at 1656 cm -1 corresponds to the amide I stretching of C=O, while the band at 1553 cm -1 (9) J o u r n a l P r e -p r o o f corresponds to the stretching or N-H deformation of amide II, and bands at 1375 cm -1 and 897 cm -1 correspond to the symmetrical deformation of amide III (Kaya et al., 2014) .

The XRD pattern of the prepared CHNW was shown in Fig. 2B . The XRD analysis was conducted to determine the structure, phase purity and degree of crystallinity of the prepared CHNW. The relative intensity of CHNW showed peaks at 2θ = 9.3, 12.7, 19.4, 20.7, 23.4, and 26.3 correspond to the reflections from the plane (020), (021), (110), (120), (130), (013) respectively. These peaks are characteristic of α-chitin (Kaya et al., 2014) . The crystallinity index ( ) of CHNW was determined using the equation (10) (Kaya et al., 2014) :

Where 110 is the highest intensity at 2θ = 20 and the amorphous diffraction intensity at 2θ = 13. The of the prepared CHNW was found to be 86%. The of CHNW was higher than that of of chitin nanofibers obtained from fungi and resting eggs J o u r n a l P r e -p r o o f (Wysokowski et al., 2013) . The high crystallinity index of CHNW was due to the removal of amorphous regions and proteins from chitin powder, which shows the effectiveness of the chemical treatment employed for the isolation of CHNW.

TEM image of the synthesized CHNW was shown in Fig. 2C . The CHNW showed rodlike morphology and the whiskers were in the nanometer range. The diameter of the individual fragments of CHNW ranges between 25-32 nm. The length of the prepared nanowhiskers was in the range of 400 nm. The aspect ratio (L/D) of CHNW was found to be in the range 12-16. The aspect ratio of the prepared CHNW was similar to the aspect ratio of chitin nanowhiskers prepared from crab shells (L/D=15) and less than that from Riftia tubes (L/D=120) by the HCl hydrolysis method (Mincea et al., 2012) . The TEM image also shows that the prepared whiskers are highly agglomerated because of inter and intramolecular hydrogen bonding present in them. (Balachandran & Bhagawan, 2012) . Differential torque is a measure of the extent of J o u r n a l P r e -p r o o f the cross-link formation and interaction between NBR & CHNW (De et al. 2012 ). The differential torque was found to be maximum for the NBR-CHNW2 composite ascribed to its high cross-link density. The increase in the differential torque of the prepared nanocomposites was due to the entanglement of nitrile rubber chains by chitin nanowhiskers. The Wolff activity coefficient ( ) corresponds to the the reinforcement effect of the CHNW in NBR. Higher the value of , higher will be the reinforcement effect of the CHNW. Consider the following equation (11):

Where ∆ is the torque increment of NBR-CHNW composites in dNm, ∆ 0 the torque increment of NBR gum in dNm, the weight of filler and the weight of the polymer.The values of were found to be positive for all the prepared NBR-CHNW composites. Maximum value of was found to be at 2 phr CHNW addition owing to the better reinforcing effect of CHNW in NBR. composite compared to NBR gum. This is the major achievement of the present technique employed. By applying latex stage processing, the chitin nanowhiskers will be more dispersed (evident from fractographic studies) in the NBR matrix compared to conventional mill mixing.

Stress transfer theory can be applied to explain the reinforcement of CHNW in NBR. When the nanowhiskers were aligned in the direction of strain, they could effectively transfer the stress from the matrix and can also prevent the propagation of microcracks (Tian et al., 2011) . The hydrogen bonding interaction between the -OH groups of CHNW and nitrile group of NBR rubber at the interface, the three-dimensional intra and inter hydrogen bonding networks between the chitin nanowhiskers, uniform dispersion and high aspect ratio of chitin nanowhiskers might be responsible for the significant improvement in the tensile strength of NBR-CHNW nanocomposites. The schematic representation of the possible mechanism of interaction between CHNW and NBR was given in Fig.3 . It was observed that the tensile strength of the prepared NBR nanocomposites decreased after 2 phr CHNW addition. At high CHNW loading, agglomeration of nanowhiskers resulted in the formation of bundles. In this case, the filler-filler interaction over ways matrix-filler interactions. Then nanowhiskers would be debonded at the interface creating voids. These voids may act as stress concentration centers that might be responsible for the deterioration of tensile strength at high CHNW loading. The elongation at break of NBR gum, NBR-CHNW2, NBR-CHNW4 and NBR-CHNW6 composites was found to be 39515%, 35411%, 36312%, 37114% respectively. The elongation at break of NBR-CHNW composites was found to be less than NBR gum. The decrease in the elongation at break of NBR-CHNW nanocomposites was due to the immobilization of the polymer chains by the composite showed a significant improvement of 58% in tear strength compared to neat NBR.

The increase in tear strength along the longitudinal direction was due to the obstruction created to the tear path by chitin nanowhiskers. Even though the tensile strength was found to be highest at 2 phr CHNW loading, the tear strength was found to be maximum at 6 phr filler loading.

Dominic C.D. et. al reported a similar trend when cellulosic nanofibers isolated from the parasitic plant Cuscuta reflexa were used as a filler in natural rubber (Dominic C.D. et al., 2020) .

The hardness of NBR-CHNW nanocomposites increased with an increase in CHNW loading due to the improved rigidity of the composites. The hardness (Shore A) of NBR gum increased from 45 to 53 by the addition of 6 phr CHNW. The abrasion resistance index (ARI) was found to be maximum for NBR-CHNW2 composite. The effective interaction between the nanowhiskers and the matrix prevents the scooping of the material as chunks by the abrader. The

high ARI value of the prepared nanocomposites indicates a longer service life. The compression set value was found to be minimum for NBR-CHNW2 composite compared to other vulcanizates ascribed to the low residual deformation, higher cross-link density and better rubbernanowhisker interactions (Rattanasom, Saowapark, & Deeprasertkul, 2007) . Table 3 . Physico-mechanical and sorption parameters of NBR-CHNW composites

The sorption parameters of NBR-CHNW composites are listed in Table 3 . The molecular transport of solvent molecules across NBR is a kinetic parameter that is mainly influenced by the free volume present in the material, the segmental mobility of NBR chains, cross-link density and the nature of the penetrant molecules. The swelling studies of the prepared NBR nanocomposites were conducted to gather valuable information about the strength of interface, 3D-network formation, the degree of dispersion of CHNW in the rubber matrix (Prasanth Kumar, Getthakumari Amma, & Sabu, 1995) .The plot of mol % uptake (Qt) vs. time 1/2 of NBR gum and NBR-CHNW composites were shown in Fig. 4A and NBR increases the cross-link density. The cross-link density has reached a maximum value at 2 phr CHNW addition, owing to the rigidity of the composite. Boonbumrung's group observed similar improvement in cross-link density when multiwalled carbon nanotube and precipitated silica were used as fillers in NBR (Boonbumrung et al., 2016) . The cross-link density was found to be decreased at 6 phr CHNW loading due to the steric hindrance created by CHNW between the molecular chains of NBR. At 6 phr CHNW loading, the increase in the number of intramolecular hydrogen bonds within CHNW may result in reduced matrix-filler interaction, leading to a detrimental effect in cross-link density. It can be related with the dispersion issue at 6 phr CHNW loading. The cross-link density measurements were in line with the differential torque values reported for the prepared composites.

The TGA curves of neat NBR and NBR-CHNW composites were shown in Fig. 4B . All the prepared composites showed single-stage degradation behavior similar to the one reported The temperature at which 50% degradation occurs (T50) for the NBR gum, NBR-CHNW2, NBR-CHNW4 and NBR-CHNW6 composite was found to be 450 C, 454 C, 460 C and 466 C respectively. The thermal analysis explicitly clarifies that there is a remarkable improvement in T50 of NBR by the addition of chitin nanowhiskers. The increase in the thermal stability of CHNW reinforced NBR composites is associated with the restricted mobility of rubber chains generated by the rigid network of chitin nanowhiskers (Liu et al., 2018) . The strong interfacial interaction between CHNW and NBR was also responsible for the improved thermal stability of the nanocomposite. The 'imprisonment' of rubber chains in the rigid three-dimensional hydrogen-bonded network substantially improve the cross-link density and rigidity of the matrix, ascribed to the better thermal stability of the composites prepared. The uniform dispersion of CHNW in NBR hinders the permeability of volatile decomposition products out of the material, slow down the pace of degradation of the composites (Visakh, Thomas, Oksman, & Mathew, 2012) . The high crystallinity index of the synthesized chitin nanowhiskers may also play a key role in improving the thermal stability of the composites. The residue at 500 C of NBR gum, NBR-CHNW2, NBR-CHNW4, NBR-CHNW6 composites was found to be 17.53%, 23.72%, 25.42% and 28.07% respectively. It was observed that the residue content at 500 C of NBR-CHNW composites increased with an increase in CHNW concentration. This suggests that chitin nanowhiskers were successfully introduced into NBR and these nanowhiskers improved the thermal stability of the composites considerably. 

The fractured surface of NBR gum seems to be smooth and cryogenic (Fig. 5A) . As CHNW loading increases the surface became irregular and rough. This shows plastic deformation and a fracture mechanism transition. The dispersion of CHNW in the NBR matrix is critical for the ultimate mechanical properties of the prepared composites (Hu et al., 2020) . The SEM image of the tensile fractured specimen of NBR-CHNW2 (Fig. 5B ) composite showed that chitin nanowhiskers were distributed in the polymer matrix homogeneously. No voids and aggregates of CHNW were seen in the sample. This indicates good compatibility between NBR and CHNW at low filler loading. The roughness, undulations and craters are seen in the tensile fractured surface of NBR-CHNW2 (Fig. 5B.) and NBR-CHNW4 composite (Fig. 5C ) specify better filler-matrix interaction and effective stress transfer. The ridged surface which is an evidence of good mechanical properties seen in the fractured specimen of NBR-CHNW2 and NBR-CHNW4 was similar to the one reported by Zhong et. al (Zhong et al., 2018) . Fig. 5D shows aggregates of CHNW, indicate the non-uniform distribution of chitin nanowhiskers in NBR at 6 phr CHNW loading. Clear phase separation and defects were observed at higher CHNW loading (Fig. 5E) 

The dynamic mechanical analysis was conducted to investigate the viscoelastic properties and the interaction between CHNW and NBR. Figure 6 shows the curves of the storage modulus ( Fig. 6A ), loss modulus (Fig. 6B ) and the loss tangent ( Fig. 6C) Chen, Zhang, Xu, & Cao, 2015) . The chitin nanowhiskers can interact with NBR in two ways. The first way is by forming hydrogen bonds with the nitrile group of NBR and -OH groups of CHNW. Secondly, CHNW can interacts with chains of NBR because of its nano-size effect forming 'restrict rubber' (Yukun Chen et al., 2015) .The effective hydrogen bonding interaction between CHNW and NBR at the interface boundary and the 3D-networks of CHNW (due to inter-and intra-molecular hydrogen bonding) provide improved wetting by polymer chains which also may be the reason for the improvement in storage modulus. The high value of storage modulus at 2 phr CHNW loading may also due to the uniform dispersion of CHNW and the high cross-link density. The value of the storage modulus is an indication of stiffness and cross-link density of composites (Cao et al., 2013) . The The loss modulus ( ") is a measure of energy lost to friction as heat in NBR-CHNW composites. The loss modulus values of NBR nanocomposites were found to be higher than that of virgin NBR. The loss modulus peaks of NBR nanocomposites were broader than NBR gum owing to the increased energy absorption of chitin nanowhiskers (Pongdong, Nakason, Kummerlöwe, & Vennemann, 2015) . The increase in the magnitude of " of the prepared nanocomposites is might be due to the energy dissipation that arises from a breakdown of CHNW transient networks and loss of trapped rubber chains on the filler surface during dynamic tests (Prasertsri & Rattanasom, 2012) . From the maxima of the loss modulus curve ( " vs. T), the glass transition temperature (Tg) of the composites was determined. The Tg of NBR, NBR-J o u r n a l P r e -p r o o f CHNW2, NBR-CHNW6 was found to be -11.15 C, -8.90C, and -9.68 °C respectively. The DMA results showed that Tg values were increased by the addition of CHNW to the NBR matrix and the maximum value was reported for 2 phr CHNW addition. The positive shift in the glass transition temperature of NBR-CHNW nanocomposites was clear evidence of effective interfacial interaction between the matrix and the filler (Chen et al., 2008) .

The loss tangent (tan δ) is the ratio of loss modulus ( ") and storage modulus ( ′). It is a parameter to determine the mechanical damping and the internal friction in the prepared NBR-CHNW composites (Dominic C.D. et al., 2020) . The temperature at which the maximum tan δ peak is considered as the glass transition temperature of the prepared composites (Dileep, Varghese, Sivakumar, & Narayanankutty, 2019) . It was observed that the addition of CHNW into the NBR matrix, decreases the loss tangent peak height and shifts the maximum tan δ peak to a higher temperature region. The magnitude of the maximum tan δ ( max ) value of NBR, NBR-CHNW2, NBR-CHNW6 was found to be 1.535, 0.985, 1.119 respectively. Balachandran et. al reported similar findings when nano-calcium carbonate is used as a reinforcing filler in NBR (Balachandran & Bhagawan, 2012) . The decrease in tanδ peak height in NBR-CHNW composites is clear evidence of the hindering of rubber molecular chains by CHNW and improved interfacial interaction (Joseph et al., 2010) . The homogenous dispersion of CHNW at 2 phr loading in the NBR matrix results in more constrained polymer domains around the surface of incorporated chitin nanowhiskers compared to NBR-CHNW6 composite. This might be the reason for the low value of max in NBR-CHNW2 composite compared to NBR-CHNW6. The Tg according to maximum tan δ of NBR gum, NBR-CHNW2, NBR-CHNW6 composite was found to be -4.09 C, 2.78 C and 1.07 C respectively. Yukun Chen et. al reported a positive shift in Tg from -1.78 C to 1.34 C by the incorporation of 15 phr nanocrystalline cellulose in NBR foam (Chen et al., 2015) . The significant improvement in Tg of NBR-CHNW composites showed the reinforcing action of CHNW (Dominic C.D. et al., 2020) .

The mechanical properties of NBR-CHNW composites were greatly influenced by the constrained region. The volume fraction of immobilized polymer chain (Cv) and adhesion parameter (A) of NBR-CHNW composites were determined according to the procedure given by Formela et. al (Formela et al., 2016) . The Cv values of NBR-CHNW2 and NBR-CHNW6 composites were found to be 0.0875 and 0.0601 respectively. The high Cv value of NBR-CHW2 J o u r n a l P r e -p r o o f composite compared to NBR-CHN6 showed the effective immobilization of polymer chains at 2 phr CHNW loading. The 'A' value of NBR-CHNW2 and NBR-CHNW6 composites were found to be -0.3449 and -0.2244 respectively. Lower the value of 'A' more will be the interfacial addition and enhanced interaction between CHNW and NBR (Formela et al., 2016) . The more negative value of 'A' obtained for NBR-CHNW2 composite showed better reinforcing action of chitin nanowhiskers at 2 phr, which is in line with the mechanical and swelling studies results.

Thus it was possible to observe that CHNW reinforced NBR composites showed better dynamic properties than NBR gum and these nanowhiskers decreases the damping of the composites significantly. The DMA analysis data was summarised in Table 4 . 

With the evolution and progress of green and sustainable nanofillers, rubber technology has been undergoing change. Novel nanomaterials are replacing conventional fillers to improve the performance of the general-purpose and specialty rubbers significantly at lower loadings. The present work has been undertaken in this context to develop acrylonitrile-butadiene rubber (NBR)-chitin nanowhisker (CHNW) composite to upgrade the processing and performance properties of NBR. Chitin nanowhiskers (CHNW) were synthesized from shrimp shells by employing the demineralization, bleaching, acid hydrolysis techniques followed by homogenization. The synthesized chitin nanowhiskers were characterized using different analytical techniques like FTIR, XRD, TEM, TGA etc. The FTIR analysis and XRD pattern of CHNW show that α chitin is present in the synthesized sample. The crystallinity index of CHNW was found to be 86%. The formation and morphology of CHNW were confirmed by TEM analysis and the aspect ratio of the whiskers was in the range 12-16. Thermogravimetric analysis shows that thermal degradation of chitin takes place in a single step and the temperature at which 50 % degradation occurs was found to be at 335 C.

Chitin nanowhisker (CHNW) reinforced NBR composites were prepared using a masterbatch of CHNW in NBR latex followed by dry rubber compounding in a two roll mills. The cure characteristics, mechanical, technological, thermal, swelling and dynamic mechanical properties were analyzed. Cure rate index decreased by the incorporation of CHNW in the NBR matrix.

The tensile strength and tear strength of NBR-CHNW2 composites showed an improvement of 116% and 54% respectively compared to neat NBR. The improvement in the tensile modulus and cross-link density of NBR-CHNW composites can be attributed to the 'caged' or 'trapped' J o u r n a l P r e -p r o o f rubber chains in the three-dimensional rigid hydrogen-bonded network of chitin nanowhiskers.

The swelling index of NBR-CHNW2 composite was found to be less than that of NBR gum. The fractographic studies of NBR-CHNW composites revealed effective stress transfer in the composite. The NBR-CHNW composites showed an ample improvement in the onset degradation temperature (Ton), the temperature at which 50 % degradation occurs (T50). The DMA studies revealed that the glass transition temperature of NBR-CHNW2 composite is about 6 C more than that of neat NBR. The lowering of tan δ peak height in NBR-CHNW composites shows the effective interaction of CHNW with NBR matrix. Based on the presented results, it can be concluded that chitin nanowhiskers seem to be promising green filler for NBR for high performance elastomeric applications. 

The authors declare no conflicts of interest.

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The authors greatly acknowledge SAIF STIC, CUSAT for chemical analysis and J J Murphy Research Centre, Kerala for rubber compounding.J o u r n a l P r e -p r o o f