key: cord-0735740-xf01ds39
authors: Inoue, Koichi
title: Potential significance of CX3CR1 dynamics in stress resilience against neuronal disorders
date: 2022-02-28
journal: Neural Regen Res
DOI: 10.4103/1673-5374.335831
sha: 9a5a7fcb914be8f3148c9a7684abca65a7860f86
doc_id: 735740
cord_uid: xf01ds39

Recent findings have implicated inflammatory responses in the central nervous system in a variety of neuropsychiatric and neurodegenerative diseases, and the understanding and control of immunological responses could be a major factor of future therapeutic strategies for neurological disorders. Microglia, derived from myelogenous cells, respond to a number of stimuli and make immune responses, resulting in a prominent role as cells that act on inflammation in the central nervous system. Fractalkine (FKN or CX3CL1) signaling is an important factor that influences the inflammatory response of microglia. The receptor for FKN, CX3CR1, is usually expressed in microglia in the brain, and therefore the inflammatory response of microglia is modified by FKN. Reportedly, FKN often suppresses inflammatory responses in microglia and activation of its receptor may be effective in the treatment of inflammatory neurological disorders. However, it has also been suggested that inflammatory responses facilitated by FKN signaling aggravate neurological disorders. Thus, further studies are still required to resolve the conflicting interpretation of the protective or deleterious contribution of microglial FKN signaling. Yet notably, regulation of FKN signaling has recently been shown to be beneficial in the treatment of human diseases, although not neurological diseases. In addition, a CX3CR1 inhibitor has been developed and successfully tested in animal models, and it is expected to be in human clinical trials in the future. In this review, I describe the potential therapeutic consideration of microglial CX3CR1 dynamics through altered FKN signaling.

"Resilience" refers to the ability to overcome and recover from stressful and/or adverse circumstances (Rutter, 1985; Grotberg, 2003) . In addition to personal qualities, resilience can be affected by family, social, and other environmental factors, and it can lead to later onset of disorders caused by factors other than personal qualities, such as neuropsychiatric disorders caused by environmental changes. For example, it has been suggested that economic problems may contribute to 20% of depressions (Dudek et al., 2021) , which should be other than personal qualities for many children. On the contrary, some of those disorders can be treated by acquiring stress resilience, which was not previously present (Feder et al., 2019) . This suggests that a well-functioning society for the treatment of diseaserelated stress resilience could be the foundation for a healthy life that does not rely on medical costs and care, which is a global problem. Even if not at the level of diseases, this competence is required more than ever in a modern and complicated society where the spread of SARS-CoV-2 infection worldwide has brought economic depression and made it difficult to imagine a promising near future with high expectations. In fact, COVID-19 can cause serious health, social, and economic crises for families, including children (Barzilay et al., 2020; Prime et al., 2020) . Stress resilience is not only related to functional mental activity but also to substantial neurological impairment. For example, higher susceptibility to stress has been reported to result in a higher prevalence of stroke and to affect not only stroke onset but also poststroke complications and recovery (Surtees et al., 2007; Bergh et al., 2014) . Resilience also plays an important role in the pathogenesis of not only stroke but also dementia and other hereditary disorders (Bergman et al., 2010; Maul et al., 2020) . In line with this, animal studies have clearly reported that stress during the perinatal period through to childhood influences the function of the hypothalamic-pituitary-adrenal axis, leading to a decrease in stress resilience in later life, and further understanding of stress resilience is an important issue for a better future (Liu et al., 1997; Sapolsky, 1997) .

Thus, the term "resilience" or "stress resilience" has become very common in scientific papers over the past decade or so. For instance, a PubMed search for "stress resilience" retrieves 37 papers in 2000, 356 in 2010, and 2190 in 2020. This may also be due to the social and medical trend of considering treatment and recovery from diseases not only from the physical but also mental aspects. In response to the question of how resilience is imparted, psychologists sometimes speak of mental strength such as endurance and perseverance, but its mechanisms have not yet been clarified at the molecular level in the field of life science (Feder et al., 2009; Menard et al., 2017) . In this regard, we have recently focused on fractalkine (FKN)/CX3CR1 signaling in microglia and found a phenomenon that may be generally involved in the resilience of function of the central nervous system (Inoue et al., 2021) . Considering the findings, in this review, I will give an overview of FKN and its signaling in microglia and review its possible significant involvement in the universal neuropsychiatric activity.

Studies cited in this narrative review published from 1985 to 2021 were searched on the PubMed database using the following keywords: fractalkine, CX3CR1, stress, resilience, microglia, inflammation, stroke, Alzheimer's disease, SARS-CoV-2, knockout mice.

FKN has been identified as a chemokine that exerts chemotactic activity on certain cells. Its receptor, CX3CR1, is expressed mainly in immune cells, such as macrophages and microglia, but it is also present in various other cells, such as neurons, astrocytes, and vascular endothelial cells (Meucci et al., 2000; Umehara et al., 2001; Limatola and Ransohoff, 2014; Lee et al., 2018) . CX3CR1 is a G protein-coupled receptor, and its activation leads to Ca 2+ influx and change in cAMP level, followed by transduction of downstream signals regarding cell differentiation and survival, such as MAPKs and Akt/PKB (Maciejewski-Lenoir et al., 1999; Meucci et al., 2000; Kansra et al., 2001; Davis and Harrison, 2006; Dorgham et al., 2009) . Its expression level is demonstrated to be increased by external stimuli in some cells (Maciejewski-Lenoir et al., 1999; Fong et al., 2000; Sung et al., 2005; O'Sullivan et al., 2016; Ho et al., 2020; Kawamura et al., 2020) , and the extent of its expression change contributes to changes in the intensity of the signal downstream of CX3CR1, as does the release of the ligand, FKN.

FKN is released from various types of cells, including neuronal, vascular endothelial, intestinal, and epithelial cells. While the stationary release is recognized, the amount of release is altered by stimuli. In both cases, FKN acts as a paracrine factor on the peripheral cells and as a hormone remotely (Dreymueller et al., 2012; Lee et al., 2018) . Thus, FKN is a released protein that is initially translated as a membrane surface protein with a transmembrane region (Bazan et al., 1997; Fong et al., 2000; Poniatowski et al., 2017; Lee et al., 2018) . It is excised at the region slightly closer to the Introduction 2153

Review N-terminal side from the transmembrane domain by serine proteases, such as ADAM17 and ADAM10, and released into the bloodstream. This soluble FKN accounts for FKN blood level. Apart from the soluble form, FKN often remains uncut and unreleased and acts on the cellular membrane. For example, CX3CR1-expressing macrophages or monocytes are trapped on membranebound (uncut) FKN-producing vascular endothelial cells, using them as a scaffold to assist penetration and migration of CX3CR1-positive cells (Bazan et al., 1997; Fong et al., 2000) . As a timely topic, it has been reported that angiotensin II causes an increase in FKN expression in vascular endothelial cells (Rius et al., 2013) . In addition, it has been suggested that FKN may play a role in severe SARS-CoV-2 infections caused by cytokine storms because of the relationship between angiotensin II and an angiotensin II-degrading enzyme ACE2, which is a target of the SARS-CoV-2 protein (Banu et al., 2020; Gracia-Hernandez et al., 2020; Rivas-Fuentes et al., 2021) .

Genetic mutations of human CX3CR1 are associated with the risk of cardiovascular and psychiatric diseases, such as schizophrenia. For example, mutation of T280M in FKN makes less activation of Ca 2+ influx-mediated cell signaling compared to wild-type FKN, resulting in weakened adhesion and invasion of CX3CR1-positive leukocytes into the vascular wall. This reduces cardiovascular diseases, such as atherosclerosis (McDermott et al., 2003) . In addition, mutation of A55T in FKN attenuates at least Akt signaling, and the changes in the signaling strength may modulate cellular function, leading to altered brain functions or development of diseases (Ishizuka et al., 2017) .

Considering that attenuated activity of FKN signals decreases disease incidence, increased activation can enhance disease development. Other than mutation, recent findings have revealed that FKN signals are abnormally evoked in human diseases such as rheumatoid arthritis, Crohn's diseases, and systemic scleroderma, and the FKN neutralizing antibody therapy may be functional (Muehlhoefer et al., 2000; Ruth et al., 2001; D'Haese et al., 2012; Luong et al., 2019; Matsuoka et al., 2021; Tanaka et al., 2021a, b) . In fact, a phase II clinical trial has been conducted using FKN neutralizing antibody in rheumatoid arthritis patients who did not respond well to methotrexate, a clinically commonly used drug. Although the patients were treated for as short as 6 months, there seemed to be a significant effect on moderation (Tanaka et al., 2021a) . These reports explore the possibility that modulation of FKN signaling could become curable therapeutic strategies for human diseases.

As mentioned above, FKN signals play critical roles in the modulation of neuronal function. The deficiency of FKN has often resulted in neurological defects, but their phenotypes are not so severe (Cook et al., 2001; Sokolowski et al., 2014; Winter et al., 2020) . CX3CR1-deficient mice have also been reported to have a variety of neurological problems. For example, inflammation-induced neurotoxicity in stroke models is reduced in CX3CR1deficient mice, whereas neurotoxicity appears to be increased by CX3CR1deficiency in Parkinson's disease and amyotrophic lateral sclerosis (ALS) models (Cardona et al., 2006; Tang et al., 2014; Wang et al., 2018) . A relationship with stress resilience has also been reported in CX3CR1-deficient mice, in which the deficiency reduces stress-induced memory and cognitive impairment and alleviates depressive symptoms (Rimmerman et al., 2017; Liu et al., 2020) .

Indeed, phenotypes exhibited in CX3CR1-deficient mice could be referable. However, it is almost impossible for real animals to lose the expression of CX3CR1 to the same extent as knock-out mice do, and therefore it is reasonable to presume that the effect of signaling intensity is attributable to changes in CX3CR1 expression level. Tables 1 and 2 list examples of reports regarding changes in microglial CX3CR1 expression levels (both protein and gene expressions) due to various stresses since it would not be meaningful to describe details of knockout mice. In the tables, brain regions ( Table  1 ) and cell types ( Table 2) , in which CX3CR1 changes, are described along with stimuli and models that cause these changes and with the subsequent results. Detection methods are also mentioned. From these tables, it should be understood that CX3CR1 is altered by a variety of stimuli, which in turn changes the phenotypes, including neurological symptoms. As shown in Table  1 , while the stimuli affecting the expression are not identified in some in vivo data, responsive stimuli are mostly identified, and the available literature implies that a variety of stimuli can modify the expression of CX3CR1. Of note, promoter analysis of human CX3CR1 has been performed, and the importance of the family of transcription factors, nuclear factor of activated T cells, has been suggested (Barlic et al., 2004) . Nuclear factor of activated T cells are mainly present in immune, cardiovascular, neuronal, and muscular cells. They are dephosphorylated by calcineurin, a phosphatase that is activated through intracellular Ca 2+ elevation, resulting in nuclear translocation and activation of downstream target gene expression (Hogan et al., 2003; Fric et al., 2012) . However, since it is activated by stimuli, such as lipopolysaccharide (LPS) and enhances gene expression (Nagamoto-Combs and Combs, 2010; Fric et al., 2012), it would not play a leading role in the response to LPS stimulation, which decreases the expression level of CX3CR1, as reported by several groups, including ours (Zujovic et al., 2000; Wynne et al., 2010; Wang et al., 2020; Inoue et al., 2021) . In addition, downstream effects, such as the survival of circumferential photoreceptors or neuronal cells, are reported (Roche et al., 2016; Wang et al., 2020) . Intriguingly, the effects of CX3CR1 regulation on disease mechanisms are likely to be true if consistent phenotypic trends are displayed between models with down-regulation of CX3CR1 and CX3CR1deficient animals. For instance, accumulation of β-amyloid is decreased in CX3CR1-deficient mice (Lee et al., 2010) , and this corresponds to Keren-Shauel's findings, as shown in Table 1 (Keren-Shaul et al., 2017) , suggesting that activation of FKN signaling may exacerbate symptoms in Alzheimer's disease. Their report indicates that attenuation of FKN signaling intensity in local microglia around the lesion makes them deviated from checkpoints that inhibit unwanted immune responses, leading to an increase in immunological activities including phagocytosis of β-amyloid. However, on the other hand, Gonzalez-Prieto et al. (2021) have reported that Alzheimer's patients have a higher expression of CX3CR1, and therefore further investigation would be expected. They have also noted microglia with lower expression of CX3CR1 accumulated in the lesion of ALS model mice. On the contrary, ALS model derived from CX3CR1-deficient mice show a more intense immune response, resulting in higher lethality. There is one thing of concern, and at first glance of the tables, it appears that CX3CR1 is often decreased by stimulation in cultured cells, while it is still increased in various in vivo models. Although the reason for this is unclear, it has been reported that the expression level of CX3CR1 itself is much higher in human microglia than in cultured cells (Gosselin et al., 2017) , and the analysis of CX3CR1 expression in microglia may not be meaningful without using in vivo reports. Therefore, it is still controversial whether changes in the CX3CR1 level are beneficial. To resolve this, recently, a CX3CR1 antagonist has become commercially available (Karlström et al., 2013; Cederblad et al., 2016; Ho et al., 2020) , and changes in phenotypes can be referrable by the antagonist. The next step is to apply this to human diseases. Most of the data shown in Table 1 are derived from animal models, and there is no guarantee that the dynamics of CX3CR1 are consistent between humans and those models. Nevertheless, if they are similar, human phenotypes such as multiple sclerosis and neurogenic hypertension could be treatable with the modification of CX3CR1 function (Ridderstad Wollberg et al., 2014; Ho et al., 2020) . To do this hereafter, changes in CX3CR1 should be examined in human microglia. If CX3CR1 in macrophages in the peripheral blood and microglia fluctuates in the same way as SGK1 does in response to glucocorticoids (Anacker et al., 2013) , then the change in CX3CR1 in the brain microglia by any stimuli can be reflected in peripheral blood, which may be used to understand and treat CX3CR1oriented human diseases with lower invasiveness.

The term "stress resilience" has become more common in medical biology papers in the last decade or so. It appears to be related not only to the development of neuropsychiatric disorders but also to exacerbations of Alzheimer's disease and post-stroke neurodegeneration, and its detailed molecular pathology is expected to be elucidated for the treatment of neurological diseases. FKN signaling is a signal involved in the immune response of microglia that affects stress resilience. Microglia express an FKN receptor, CX3CR1, and exchange signals with ligand-expressing neurons and other cells. The amount and form of the ligand FKN (membranebound or soluble) can inflect the stimulation of microglia and modulate its effects, ultimately modifying brain and nerve functions. Changes in CX3CR1 expression in microglia have been reported often, and it is generally accepted that its expression is altered by various stimuli, which may also occur in human diseases. At the level where mental function is involved, there have been no studies utilizing neutralizing antibodies or the inhibitor, but as in the case of rheumatoid arthritis mentioned above, its increased activity may lead to exacerbation of the condition. We found that the expression level of CX3CR1 in microglia is reduced by stimulation with alcohol or LPS, albeit in cultured cells (Inoue et al., 2021) . Since it is often reported that stimulation of CX3CR1 suppresses microglial inflammatory responses in common LPSbased microglial immune research, we consider that if a similar situation occurs in vivo and in humans, various stresses may cause suppression of CX3CR1 expression and the consequent FKN signaling function in microglia, thereby reducing the ability of microglia to suppress runaway. Unfortunately, however, there is currently no clear direction as to whether this variation is uniformly good or bad for certain conditions. Nonetheless, this also being the case in humans, if we can prove beneficial changes in CX3CR1 for each individual disease by adjusting the life environment outside of clinical treatment, such as diet and exercise, then we may be able to acquire beneficial stress resilience through FKN signaling. 

Role for the kinase SGK1 in stress, depression, and glucocorticoid effects on hippocampal neurogenesis

Protective role of ACE2 and its downregulation in SARS-CoV-2 infection leading to macrophage activation syndrome: therapeutic implications

Interleukin (IL)-15 and IL-2 reciprocally regulate expression of the chemokine receptor CX3CR1 through selective NFAT1-and NFAT2-dependent mechanisms

2020) Resilience, COVID-19-related stress, anxiety and depression during the pandemic in a large population enriched for healthcare providers

A new class of membrane-bound chemokine with a CX3C motif

Stress resilience in male adolescents and subsequent stroke risk: cohort study

Maternal prenatal cortisol and infant cognitive development: moderation by infant-mother attachment

Control of microglial neurotoxicity by the fractalkine receptor

Cx3cr1-deficiency exacerbates alpha-synuclein-A53T induced neuroinflammation and neurodegeneration in a mouse model of Parkinson's disease

AZD8797 is an allosteric non-competitive modulator of the human CX3CR1 receptor

Microglia in the RVLM of SHR have reduced P2Y12R and CX3CR1 expression, shorter processes, and lower cell density

Generation and analysis of mice lacking the chemokine fractalkine

Therapeutic potential of the chemokine-receptor duo fractalkine/CX3CR1: an update

Proline 326 in the C terminus of murine CX3CR1 prevents G-protein and phosphatidylinositol 3-kinase-dependent stimulation of Akt and extracellular signal-regulated kinase in Chinese hamster ovary cells

Deficient CX3CR1 signaling promotes recovery after mouse spinal cord injury by limiting the recruitment and activation of Ly6C lo /iNOS + macrophages

Sex differences in microglial CX3CR1 signalling determine obesity susceptibility in mice

An engineered CX3CR1 antagonist endowed with anti-inflammatory activity

Lung endothelial ADAM17 regulates the acute inflammatory response to lipopolysaccharide

Neurobiology of resilience in depression: immune and vascular insights from human and animal studies

Psychobiology and molecular genetics of resilience

The biology of human resilience: opportunities for enhancing resilience across the life span

Ultrastructure and function of the fractalkine mucin domain in CX3C chemokine domain presentation

Effect of electroacupuncture on the cervicospinal P2X7 receptor/fractalkine/CX3CR1 signaling pathway in a rat neck-incision pain model

Microglial CX3CR1 production increases in Alzheimer's disease and is regulated by noradrenaline

An environment-dependent transcriptional network specifies human microglia identity

Targeting macrophages as a therapeutic option in coronavirus disease 2019

Resilience for Today: Gaining Strength from Adversity

CX3CR1-microglia mediates neuroinflammation and blood pressure regulation in the nucleus tractus solitarii of fructose-induced hypertensive rats

Transcriptional regulation by calcium, calcineurin, and NFAT

Expression of fractalkine (CX3CL1) and its receptor, CX3CR1, during acute and chronic inflammation in the rodent CNS

Modulation of inflammatory responses by fractalkine signaling in microglia

Rare genetic variants in CX3CR1 and their contribution to the increased risk of schizophrenia and autism spectrum disorders

Phosphatidylinositol 3-kinase-dependent extracellular calcium influx is essential for CX3CR1-mediated activation of the mitogen-activated protein kinase cascade

Substituted 7-amino-5-thio-thiazolo[4,5-d]pyrimidines as potent and selective antagonists of the fractalkine receptor (CX3CR1)

Reduced brain fractalkine-CX3CR1 signaling is involved in the impaired cognition of streptozotocin-treated mice

A unique microglia type associated with restricting development of Alzheimer's disease

Tissue-specific role of CX3CR1 expressing immune cells and their relationships with human disease

CX3CR1 deficiency alters microglial activation and reduces beta-amyloid deposition in two Alzheimer's disease mouse models

Modulating neurotoxicity through CX3CL1/CX3CR1 signaling

Evidence for a protective role of the CX3CL1/CX3CR1 axis in a model of amyotrophic lateral sclerosis

Maternal care, hippocampal glucocorticoid receptors, and hypothalamicpituitary-adrenal responses to stress

Involvement of CX3CL1/CX3CR1 in depression and cognitive impairment induced by chronic unpredictable stress and relevant underlying mechanism

CX3CR1 in microglia regulates brain amyloid deposition through selective protofibrillar amyloid-β phagocytosis

Inhibition of the progression of skin inflammation, fibrosis, and vascular injury by blockade of the CX3CL1/CX3CR1 pathway in experimental mouse models of systemic sclerosis

Characterization of fractalkine in rat brain cells: migratory and activation signals for CX3CR-1-expressing microglia

MicroRNA-195 prevents hippocampal microglial/macrophage polarization towards the M1 phenotype induced by chronic brain hypoperfusion through regulating CX3CL1/CX3CR1 signaling

Phase 1 study on the safety and efficacy of E6011, antifractalkine antibody, in patients with Crohn's disease

Genetics of resilience: Implications from genome-wide association studies and candidate genes of the stress response system in posttraumatic stress disorder and depression

Chemokine receptor mutant CX3CR1-M280 has impaired adhesive function and correlates with protection from cardiovascular disease in humans

Immune and neuroendocrine mechanisms of stress vulnerability and resilience

Expression of CX3CR1 chemokine receptors on neurons and their role in neuronal survival

Fractalkine is an epithelial and endothelial cell-derived chemoattractant for intraepithelial lymphocytes in the small intestinal mucosa

Microglial phenotype is regulated by activity of the transcription factor, NFAT (nuclear factor of activated T cells)

Fractalkine shedding is mediated by p38 and the ADAM10 protease under pro-inflammatory conditions in human astrocytes

Analysis of the role of CX3CL1 (fractalkine) and its receptor CX3CR1 in traumatic brain and spinal cord injury: insight into recent advances in actions of neurochemokine agents

Risk and resilience in family well-being during the COVID-19 pandemic

Pharmacological inhibition of the chemokine receptor CX3CR1 attenuates disease in a chronic-relapsing rat model for multiple sclerosis

The hippocampal transcriptomic signature of stress resilience in mice with microglial fractalkine receptor (CX3CR1) deficiency

Arterial and venous endothelia display differential functional fractalkine (CX3CL1) expression by angiotensin-II

Could SARS-CoV-2 blocking of ACE2 in endothelial cells result in upregulation of CX3CL1, promoting thrombosis in COVID-19 patients?

Progesterone attenuates microglial-driven retinal degeneration and stimulates protective fractalkine-CX3CR1 signaling

Fractalkine, a novel chemokine in rheumatoid arthritis and in rat adjuvant-induced arthritis

Resilience in the face of adversity. Protective factors and resistance to psychiatric disorder

The importance of a well-groomed child

Cathepsin S contributes to microglia-mediated olfactory dysfunction through the regulation of Cx3cl1-Cx3cr1 axis in a Niemann-Pick disease type C1 model

Fractalkine is a "find-me" signal released by neurons undergoing ethanol-induced apoptosis

Protective effect of α-lipoic acid in lipopolysaccharide-induced endothelial fractalkine expression

Adaptation to social adversity is associated with stroke incidence: evidence from the EPIC-Norfolk prospective cohort study

A phase 2 study of E6011, an anti-fractalkine monoclonal antibody, in patients with rheumatoid arthritis inadequately responding to biological disease-modifying antirheumatic drugs

Efficacy and safety of E6011, an anti-fractalkine monoclonal antibody, in patients with active rheumatoid arthritis with inadequate response to methotrexate: results of a randomized, double-blind, placebo-controlled phase II study

CX3CR1 deficiency suppresses activation and neurotoxicity of microglia/macrophage in experimental ischemic stroke

Fractalkine and vascular injury

Ischemia-induced neuronal cell death is mediated by chemokine receptor CX3CR1

Disease progression-dependent expression of CD200R1 and CX3CR1 in mouse models of Parkinson's disease

Two forms of CX3CL1 display differential activity and rescue cognitive deficits in CX3CL1 knockout mice

Protracted downregulation of CX3CR1 on microglia of aged mice after lipopolysaccharide challenge

Vascular smooth muscle cell signaling mechanisms for contraction to Angiotensin II and Endothelin-1

Role of fractalkine/CX3CR1 interaction in light-induced photoreceptor degeneration through regulating retinal microglial activation and migration

Fractalkine modulates TNF-α secretion and neurotoxicity induced by microglial activation