key: cord-0731748-ksx4bmp8 authors: Colson, Philippe; La Scola, Bernard; Esteves-Vieira, Vera; Ninove, Laetitia; Zandotti, Christine; Jimeno, Marie-Thérèse; Gazin, Céline; Bedotto, Marielle; Filosa, Véronique; Giraud-Gatineau, Audrey; Chaudet, Hervé; Brouqui, Philippe; Lagier, Jean-Christophe; Raoult, Didier title: Letter to the editor: Plenty of coronaviruses but no SARS-CoV-2 date: 2020-02-27 journal: Euro Surveill DOI: 10.2807/1560-7917.es.2020.25.8.2000171 sha: 629b679c1372ac0d4e24c5213edbebe94d5ab877 doc_id: 731748 cord_uid: ksx4bmp8 nan To the editor: We read with interest the recent article by Reusken et al. about laboratory readiness for molecular testing of the novel coronavirus 2019, recently named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in expert laboratories in 30 European countries [1] . At the time of the Middle East respiratory syndrome (MERS)-coronavirus epidemic in 2012, we had highlighted the absence of diagnosis of this virus among travellers returning from the Hajj pilgrimage, which contrasted with the considerable anxiety relating to this emerging infection and its risk of importation and spread in mainland France [2] . Instead of MERS-CoV, influenza A and B viruses had been detected. This illustrated the major disconnect between the fear of a hypothetical spread in France of a virus emerging in the Middle East and the reality of the absence of diagnosed cases, while concomitantly the very real and high incidence of respiratory viruses common worldwide and in our country and their associated mortality appeared largely neglected. Seven years later, the emergence of SARS-CoV-2 in December 2019 reproduced this pattern of disproportionate fear of importation and spread of infections in mainland France while the cases reported worldwide remain almost only localised in China as only 34 people died of this disease (Covid-19) outside China as at 25 February 2020 [3] . In our reference institute for infectious diseases, we have been implementing since the end of January 2020 PCR detection of SARS-CoV-2 RNA using several systems, including those released at the European level [4] . In total, we have tested to date (as at 19 February 2020) 4,084 respiratory samples by PCR and all the tests have been negative for SARS-CoV-2. These tests were carried out on the samples of 32 suspected SARS-CoV-2 cases, 337 people repatriated at the beginning of February 2020 from China tested twice, 164 patients who died in public hospitals in Marseille between 2014 and 2019 of whom at least one respiratory sample had been sent to our laboratory, and they also included 3,214 respiratory samples sent since January 2020 to our laboratory to search for a viral aetiology. In striking contrast, we have tested 5,080 respiratory samples for various suspected respiratory viral infections since 1 January 2020 and identified in 3,380 cases respiratory viruses. In decreasing order of frequency, they were: influenza A virus (n = 794), influenza B virus (n = 588), rhinovirus (n = 567), respiratory syncytial virus (n = 361), adenovirus (n = 226), metapneumovirus (n = 192), enterovirus (n = 171), bocavirus (n = 83), parainfluenza virus (n = 24), and parechovirus (n = 8). Among the diagnosed viruses, there were also 373 common human coronaviruses (HCoV), including 205 HCoV-HKU1, 94 HCoV-NL63, 46 HCoV-OC43, and 28 HCoV-229E [5] . Furthermore, analysis of the mortality associated with these viruses has been able to show that since 1 January 2020, one patient died after being diagnosed with HCoV-HKU1, and respiratory viruses were found in 13 other patients who died, which included influenza A virus (3 cases), respiratory syncytial virus (3 cases), rhinovirus (5 cases), adenovirus (1 case) and metapneumovirus (1 case). Retrospectively, analysis of deaths in patients who have had a respiratory sample has shown that at least nine patients have died between 2017 and 2019 after being diagnosed with one of the four coronaviruses commonly circulating in humans [6] . Thus, it is surprising to see that all the attention focused on a virus whose mortality ultimately appears to be of the same order of magnitude as that of common coronaviruses or other respiratory viruses such as influenza or respiratory syncytial virus, while the four common HCoV diagnosed go unnoticed although their incidence is high. In fact, the four common HCoV are often not even identified in routine diagnosis in most laboratories, although they are genetically very different from each other [7] and associated with distinct symptomatology [8] . On Behalf Of Evd-LabNet And Erli-Net. Laboratory readiness and response for novel coronavirus (2019-nCoV) in expert laboratories in 30 EU/EEA countries From the Hajj: it's the flu, idiot Coronavirus disease 2019 (COVID-19) Situation Report -36. Geneva: WHO; 2020 Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR Marseille: IHU Méditerranée Infection; 19/02/2020. French Marseille: IHU Méditerranée Infection Origin and evolution of pathogenic coronaviruses Epidemiology and clinical characteristics of human coronaviruses OC43, 229E, NL63, and HKU1: a study of hospitalized children with acute respiratory tract infection in Guangzhou, China This work was supported by the French Government under the "Investments for the Future" programme managed by the National Agency for Research (ANR), Méditerranée-Infection 10-IAHU-03. Testing of repatriates was approved by the ethical board of the Committee for the Protection of Persons (CPP Ile de France VI dated 06-02-2020). None declared. This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence and indicate if changes were made.Any supplementary material referenced in the article can be found in the online version.