key: cord-0727947-tg38cghx authors: Torre, D; Speranza, F; Martegani, R title: Impact of highly active antiretroviral therapy on organ‐specific manifestations of HIV‐1 infection date: 2005-03-08 journal: HIV Med DOI: 10.1111/j.1468-1293.2005.00268.x sha: 3980df4b8b9acc4f5dfbeffddce2fa8cf1042c20 doc_id: 727947 cord_uid: tg38cghx In the last 10 years, interesting results have been reported concerning the impact of highly active antiretroviral therapy (HAART) on the changing pattern of organ‐specific manifestations of HIV‐1 infection. There has been a clear step‐wise reduction in the incidence of several opportunistic infections (OIs), particularly Pneumocystis carinii pneumonia, whereas a nonsignificant reduction in incidence has been observed for other organ‐specific diseases, including invasive cervical cancer and Hodgkin disease. In addition, several organ‐specific manifestations, including HIV‐associated nephropathy, wasting syndrome and cardiomiopathy, are a direct consequence of damage by HIV‐1, and so HAART may have a therapeutic effect in improving or preventing these manifestations. Finally, the introduction of HAART has seen the emergence of several complications, termed immune reconstitution inflammatory syndrome, which includes OIs such as cytomegalovirus vitritis, Mycobacterium avium complex lymphadenitis, paradoxical responses to treatment for tuberculosis, and exacerbation of cryptococcosis. Because not all HIV‐1 organ‐specific manifestations are decreasing in the HAART era, this review will analyse the influence of HAART on several organ‐specific manifestations, and in particular OIs related to several organs, cerebral disorders and HIV‐1‐related neoplasia. The natural history of HIV-1 infection has changed in the era of highly active antiretroviral therapy (HAART), with the incidence of opportunistic infections (OIs) associated with HIV-1 infection and AIDS-related deaths having significantly decreased [1, 2] . However, the decline in the incidence of AIDS-defining illnesses has not been paralleled by a change in the spectrum or frequency of AIDSdefining illnesses [3] . Nevertheless, the percentage of hospitalized HIV-1-infected patients who have OIs has remained stable in the HAART era [4] , and OIs seem to appear with the same frequency as in the pre-HAART era. Organ-specific manifestations in the pre-HAART era The clinical manifestations of HIV-1 disease affect multiple organ systems. The severity of each manifestation varies by organ system and can be related to HIV-1 replication in infected tissue, concomitant OI of the organ, or an adverse end-organ drug effect. Before the introduction of HAART at the end of 1996, the clinical spectrum of AIDS was wide, and practically all organs were involved during the HIV-1 infection. The most common organ-specific manifestations in HIV-1-infected patients included AIDS-defining illnesses of the lung (Pneumocystis carinii pneumonia), brain (Toxoplasma encephalitis and HIV-1 encephalitis), heart (pericarditis), gut [candidal and cytomegalovirus (CMV) infection, and oesophagitis], kidney (focal glomerulosclerosis), skin (Kaposi's sarcoma), and lymphoid tissue (non-Hodgkin lymphoma) ( Tables 1, 2 and 3 ). In addition, several AIDS-defining illnesses were predictors of poorer survival in the pre-HAART era, such as CMV disease, HIV-1 encephalopathy and Toxoplasma encephalitis. the incidence of several OIs, particularly Pneumocystis carinii pneumonia (PCP), while a nonsignificant reduction in incidence was observed for other organ-specific diseases, including invasive cervical cancer and Hodgkin disease (HD) [7] . Torres et al. [8] observed that, at a New York City hospital, the percentage of hospitalized HIV-1infected patients who had OIs was unchanged in the HAART era. We reviewed several large studies that evaluated the effectiveness of HAART in terms of the incidence of AIDS-defining illnesses. As shown in Table 2 , these studies produced contrasting results; while some studies reported a reduction in the incidence of AIDS-defining illnesses, others reported no change or an increase in the incidence of AIDS-defining illnesses, particularly OIs [3, 6, 9, 10] . In particular, Dore et al. [10], in a large recent study, observed that HAART had an impact on Mycobacterium avium complex (MAC) infection and cytomegalovirus (CMV) disease, whereas they noted a significant increase in PCP and tuberculosis. Lederberger et al. [11] evaluated [12] , and inflammation and fibrosis from HIV and HCV coinfection worsened during antiretroviral therapy [13] . It should be noted that most retrospective and prospective studies concerning the impact of HAART on the incidence of AIDS-defining illnesses did not fully clarify the frequency of AIDS diagnoses and the frequency of mortality related to these illnesses. However, the introduction of HAART has seen the emergence of several complications, termed immune reconstitution inflammatory syndrome (IRIS), including CMV vitritis, MAC lymphadenitis, paradoxical responses to treatment for tuberculosis, and exacerbation of cryptococcosis [6] . This syndrome seems to be an unmasking of an undiagnosed OI, or an exacerbation of a diagnosed OI in the setting of improved immune function that contributes to the pathogenesis of the OI. Although OIs produce particular organ-specific manifestations during the course of the disease, other organspecific manifestations may be sustained by the HIV-1 itself, such as HIV-associated nephropathy (HIVAN), cardiomiopathy, HIV-1 encephalopathy and wasting syndrome. The widening of the clinical spectrum of organ-specific manifestations during the HAART era is also influenced by other factors, including undiagnosed HIV-1 infection and an increasing proportion of patients diagnosed late with HIV-1. With the introduction of HAART and its widespread use over the last few years, several concerns regarding organspecific manifestations have been raised which are still to be addressed. One issue which has to some extent been resolved is the interruption of primary and secondary prophylaxes against opportunistic pathogens, including PCP, MAC, Cryptococcus neoformans and Toxoplasma gondii in patients successfully treated with HAART. Some concerns are yet to be addressed regarding interruption of maintenance therapy, including disseminated MAC infection, cerebral toxoplasmosis and extrapulmonary cryptococcosis in patients under treatment with HAART. In this review, we analyse the influence of HAART on several organ-specific manifestations, particularly OIs, cerebral disorders and HIV-1-related malignancies, as not all HIV-1 organ-specific manifestations are decreasing in the HAART era. In the last few years, it has become apparent that the lung is an important niche for the replication of HIV-1, which may have implications for HAART. The lung is a major site for opportunistic pathogens, such as PCP, Mycobacterium tuberculosis and pyogenic bacteria. Wolff et al. [14] have clearly shown that PCP has been a less common diagnosis since HAART became available, whereas bacterial pneumonia has been more common in patients treated with HAART than in those treated in the pre-HAART era. In contrast, another study found a significant decrease in bacterial pneumonia in patients treated with HAART [15] . No difference was found in the incidence of CMV pneumonia in HIV-1-infected patients receiving or not receiving HAART [14] . In addition, Jones et al. [16] observed that the risk for tuberculosis was much lower among patients treated with HAART, and they pointed out that widespread use of HAART does reduce the risk of tuberculosis, and may help bring about further declines in tuberculosis among HIV-1-infected patients. Finally, the impact of HAART on pulmonary malignancies has not been fully investigated. Wolff et al. [14] showed a significant increase in non-Hodgkin lymphoma in patients receiving HAART compared with the pre-HAART era. Similarly, the incidence of HIV-1-related lung cancer increased from 0.8/100 patient-years in the pre-HAART era to 6.7/100 patient-years in the post-HAART era [17] . HIV-1 infection is often complicated by neurological disorders in the advanced stage of the disease. Some investigators have reported a decrease in the incidence and prevalence of OIs of the central nervous system (CNS), which may be correlated with the use of HAART since 1996 [18, 19] , whereas during the pre-HAART era neurological disorders were the initial manifestations of AIDS in 7-20% of patients [20] . In a recent study, Neuenburg et al. [21] confirmed a decreased incidence of OIs of the CNS, including toxoplasmosis, CMV infection and cryptococcosis. In particular, a progressive reduction in the incidence of cerebral toxoplasmosis was observed in the HAART era [21] [22] [23] , but this infection still tended to occur regularly in patients with advanced immunosuppression, especially in those patients who failed HAART. The impact of HAART has also been investigated with regard to other neurological disorders, such as HIV-1 encephalopathy, primary CNS lymphoma, progressive multifocal leukoencephalopathy (PML) and distal symmetric polyneuropathy. An autopsy study showed increased HIV-1 encephalopathy from 1982 to 1993, and persistence of mild HIV-1 encephalopathy from 1994-1998 [24] . Thus, in the HAART era there has been a persistent increase in mild and moderate HIV-1 encephalopathy, whereas severe HIV-1 encephalopathy, which was uncommon in the pre-HAART era, has not been observed at all in the HAART era [21] . The trend towards an amelioration of HIV-1 encephalopathy in the HAART era might be explained by direct inhibition of HIV within the brain, as well as improvement of immunological markers, by HAART; moreover, HAART may lead to suppression of inflammatory neurotoxins within the brain [25] . HAART prolongs life by restoring immune responses to non-HIV-1 pathogens, but does not prevent direct HIV-1related pathology in the brain, so long-term survival appears to increase the risk of HIV-1 encephalopathy. Maschke et al. [23] have observed a significant reduction in the prevalence of HIV-1-associated distal symmetric polyneuropathy during the HAART era. These investigators suggested that HAART may be effective in this pathology through the suppression of macrophage activation and neurotoxin production in the peripheral nerve [26] . Another interesting aspect of neurological disorders in the HAART era is the decline of primary CNS lymphoma [21, 27] . In addition, immune recovery induced by HAART in patients with primary CNS lymphoma leads to improvement in the survival of these patients [28] . Whereas the incidences of most neurological disorders have reduced since HAART was introduced, the incidence of PML has not significantly changed between the pre-HAART and HAART eras [21, 29, 30] . In addition, PML outcome has been found to be poor in both HAART-naïve and HAART-experienced patients who responded to anti-HIV treatment [29] . It should be noted that PML has been associated with immune reconstitution [6] , and that immune reconstitution as a result of HAART does not, paradoxically, worsen the course of PML [30] . Finally, psychiatric disorders, including acute psychosis, mainly develop in patients with advanced HIV-1 infection, with a wide incidence range of 0.2-15% [31] . This wide range of incidence of psychiatric disorders is probably a result of varying clinical selection criteria for the patient population, the fact that these studies were mainly conducted in psychiatric wards, and finally varying diagnostic criteria for mental illnesses. In a retrospective study, we evaluated the impact of HAART on acute psychosis [32] . Our study showed a significant increase of acute psychosis during the HAART period. Since HAART has prolonged life expectancy, it can be postulated that the risk of mental disorders may reflect the proportion of HIV-infected individuals suffering from various chronic mental conditions. Cardiac involvement is commonly reported in HIV-1infected patients, especially in those in the advanced stage of the disease [33] . Before the HAART era, pericarditis was the most frequent clinical cardiac involvement observed in the AIDS population [34] , caused by specific organisms such as M. tuberculosis, Streptococcus pneumoniae or Staphylococcus aureus [35] . In addition, cardiac involvement with cardiomyopathy, myocarditis and endocarditis was often related to opportunistic pathogens [36] . The course of HIV-1 infection, and particularly cardiac involvement, has been profoundly modified by the introduction of HAART, with OIs being less frequent and survival having been prolonged. Pugliese et al. [37] , in a retrospective study, showed cardiac involvement in 282 of 544 patients (51.8%) before the HAART era, but in only 93 of 498 patients (18.6%) during the HAART era. A significant reduction of pericarditis, dilated cardiomyopathy, and ischemia was observed in patients treated with HAART, although pulmonary hypertension significantly increased during the HAART era. However, pericarditis remains the commonest cardiac involvement in the HAART era [37] . In the last 7 years, the increased and long-term use of protease inhibitors (PIs) has raised concerns about an increased risk of coronary heart disease [38] . Retrospective studies in large numbers of patients have produced conflicting results [39] [40] [41] [42] . More recently, in a large, prospective observational study of about 23 500 HIV-1infected patients, Friiss-Moller et al. [43] observed a 27% increase of risk factors for myocardial infarction for each year of HAART exposure up to 7 years. They also pointed out that overall myocardial infarction remains relatively rare (126 events in 23 500 patients). In the last 10 years, renal diseases have become frequent complications of HIV-1 infection, and the number of patients starting dyalisis has increased by 20% per year [44] . In addition, HIV-1-related renal diseases were found to be the forth leading cause of end-stage renal disease among black men aged 20-64 years [45] . HIV-associated nephropathy (HIVAN) is the most common HIV-1-related renal disease [46] , and it is probably related to a direct effect of HIV-1 on the kidney. Other renal lesions are membranous glomerulonephritis, immunoglobulin A (IgA) nephropathy, and haemolytic uremic syndrome [46] [47] [48] . No effective treatment has been found, and the majority of patients with HIVAN become dyalisisdependent. Several case reports and case series show the beneficial effect of antiretroviral therapy on slowing the progression of renal diseases. Monotherapy with zidovudine resulted in better renal outcomes in patients with HIVAN [49] . Recent case reports support the effectiveness of HAART, particularly PIs [50, 51] , in renal function in one patient with HIVAN, and in two patients with membranous nephropathy, respectively. More recently, Szczech et al. [52] reviewed the clinical courses of 19 HIV-1-infected patients with HIVAN or other HIV-related renal diseases. They showed beneficial effects of PIs in association with prednisone on the progression of these nephropathies. From these studies, it is difficult to determine whether this benefit is related to the specific use of antiretroviral drugs in suppressing viral replication, inasmuch as HIVAN involves a direct effect of HIV-1 expression in cells of the kidney [53] . In the pre-HAART era, the prognosis for HIV-1-infected patients with end-stage renal disease was poor, with the mortality rate reaching 50% 1 year after starting dyalisis [54] . In the HAART era, the mortality rate is still more than 30% 1 year after starting dyalisis [45] . Despite the potential beneficial effects of HAART, Schwartz et al. [55] estimate that the exponential increase in the number of patients with improved quality of life and life expectancy will result in a similar expansion in the number of HIV-1-infected patients who progress to end-stage renal disease. However, it should be noted that some antiretroviral drugs, including a PI, indinavir, and a nucleoside reverse transcriptase inhibitor (NRTI), tenofovir, may cause nephropathy. In particular, renal intolerance of indinavir is a rare but important complication in HIV-1-infected patients, and several cases of acute renal failure, renal atrophy and interstitial nephritis have been reported [56] . Karras et al. [57] have recently reported three cases of renal toxicity associated with the use of tenofovir, including renal failure, proximal tubular dysfunction, and nephrogenic diabetes insipidus. Gastrointestinal disease has been one of the most common features of HIV-1 infection, and since HIV-1 particularly affects the mucosal immune system, the gastrointestinal tract is a target for several OIs as well as various HIV-1associated diseases. OIs are the most frequent gastrointestinal manifestations of AIDS, including oesophageal disease, enterocolitis, and biliary tract and pancreatic diseases, and remain a major cause of morbidity and mortality in HIV-infected patients [58, 59] . Before the introduction of HAART, the most common oesophageal disease was oesophagitis caused by Candida albicans, CMV and herpes simplex virus (HSV). Diarrhoea is frequently observed during the clinical course of the disease [60] ; the most common pathogens isolated were protozoa such as Criptosporidium and Microsporidium, viruses such as CMV, adenovirus and coronavirus [61] , and bacteria, including Clostridium difficile, Shigella flexeneri, Salmonella spp., and Campylobacter spp. [60, 62] . Monkemuller et al. [60] have evaluated the effect of HAART on the prevalence of gastrointestinal OIs in HIV-1infected patients evaluated endoscopically over a 3-year period. They observed a marked reduction of gastrointestinal OIs, including CMV infection, oesophageal candidiasis, bacterial colitis and C. difficile colitis, in HIV-1-infected patients, and more interestingly the clearance of OIs in these patients after HAART therapy was achieved despite persistently low CD4 cell counts. Several authors have also shown that HAART can restore immunity to Cryptosporidium parvum and Enterocytozoon bieneusi in HIV-1-infected patients, resulting in complete clinical, microbiological and histological resolution [63, 64] . With increasing use of HAART, gastrointestinal OIs are becoming uncommon findings for patients with AIDS. However, it should be remembered that OIs may still occur in AIDS, especially when patients access care late in the course of the disease, or are not adherent with therapy, or when resistance of HIV to antiretroviral drugs develops. It is well known that most antiretroviral drugs are often associated with gastrointestinal side-effects, including nausea, vomiting and diarrhoea, and these effects are the most frequently cited reason for discontinuation of HAART [65] . Thus, in the era of HAART, diarrhoea still occurs as a manifestation in HIV-1 patients; despite the relatively small percentage of hospitalizations due to diarrhoea, this clinical manifestation can have a debilitating impact on HIV-1-infected patients [66] . Hepatobiliary manifestations of HIV-1 infection are common but rarely fatal. The liver is a reservoir for HIV-1 infection and a target organ for several OIs. Before the introduction of HAART, mycobacterial infection of the liver was the most common infection diagnosed on liver biopsy in HIV-1-infected patients. MAC and M. tuberculosis were more frequently isolated in patients with a CD4 cell counts lower than 50 cells/mL [67] . In addition, the liver is involved in other opportunistic infections caused by CMV, T. gondii, Leishmania spp., and C. neoformans. After the introduction of HAART, as discussed above, a dramatic reduction was observed in disseminated infections, mainly caused by Mycobacterium spp., CMV and C. neoformans, and consequently a reduction in liver OIs was also observed. However, a critical issue remains concerning coinfection of HIV with various hepatotropic viruses in the era of HAART. Several studies have shown that HIV-1 affects outcome for patients with chronic HBV and HCV infections [68, 69] . In fact, HIV-1 infection, along with an impaired cellmediated response, produces increased HBV replication, increased HBV DNA and consequently a reduced response to interferon-alpha therapy. The impact of HAART on chronic viral hepatic infections has been extensively investigated for HCV infection, and to a lesser extent for HBV infection. Restoration of immunity to chronic HBV infection may occur after HAART, yet HAART may increase the rate of progression of liver disease in HIV-1-infected patients, through an increase in cytotoxic T cells, PI liver toxicity or transaminase levels [70] . Moreover, HAART has been suggested to provoke HBV reactivation, either as a result of direct effects of HAART or the accumulation of immune escape mutants [71] . HAART has improved survival rates among HIV-1infected patients, but increased mortality related to progression of chronic HCV infection and liver failure has been reported [69, 72] . There are conflicting data on the effect of HAART on the course of HCV viraemia and liver disease in HIV-1-infected patients. Some studies have shown no significant changes in HCV RNA and alanine aminotransferase serum levels during HAART [73, 74] . We have confirmed these results, and we also observed that, in a group of patients who showed increased levels of alanine aminotransferase during HAART, HCV RNA levels did not further increase [75] . In contrast, other investigators reported transient or persistent increases in HCV load during HAART [76, 77] . In particular, Babik et al. [78] demonstrated that, after long-term HAART, patients had a higher HCV load and increased quasispecies diversity, suggesting that HAART drives HCV to evolve more rapidly in an attempt to create escape mutants. Other investigators showed that HAART induces a decline or clearance of HCV RNA serum levels, and this is an encouraging finding for coinfected patients because responsiveness to interferon-alpha therapy seems to be inversely correlated with serum HCV RNA levels [79, 80] . Finally, we may speculate that effects of HAART associated with different characteristics of the immune response and HCV disease may explain the conflicting results that have been reported to date. Oral and cutaneous manifestations are present in virtually all patients with HIV-1 infection at some point in the course of their disease. The clinical spectrum of HIV-1related oral and cutaneous manifestations has changed over the last 15 years through increasing use of prophylactic drugs to prevent AIDS-related OIs, and, most importantly, through availability of HAART. The most frequent oral manifestation observed in HIV-1infected patients before the introduction of HAART was oral candidiasis, while other common oral manifestations included oral hairy leukoplakia, herpese simplex labialis, gengivitis-periodontitis, and KS [81, 82] . KS has an high incidence in HIV-1-infected patients, and it is the most common cancer occurring in these patients [83] . In the last 5 years, several studies have evaluated the impact of HAART on the incidence of cancers in HIV-1infected patients. They showed a dramatic decline in the incidence of KS in patients treated with HAART, and this decline may continue as new, more effective antiretroviral agents are developed and widely used [84] [85] [86] . However, it is still unclear whether the treatment effect results from the direct action of antiretroviral agents on HIV-1, which is known to trigger KS, or represents a direct antiviral potency against human herpesvirus 8. Sgadari et al. [87] showed that administration of the PI indinavir or saquinavir to nude mice blocked the development and induced regression of angio-proliferative KS-like lesions promoted by primary human KS cells. Oral manifestations of HIV-1 are changing in the era of HAART; in particular, oral candidiasis, herpes simplex labialis, oral KS and periodontal disease decreased by more than 30% after the introduction of HAART [82, 88] . However, HSV infection, salivary-gland disease and oral warts, along with oral candidiasis, appear to persist with HAART therapy [89, 90] . The spectrum of dermatological findings related to HIV-1 also includes a variety of cutaneous disorders. The most frequent diagnoses before the era of HAART were seborrheic dermatitis, dermatophytosis of the skin, folliculitis, papular pruritic dermatitis, herpes simplex and zoster virus infections, and scabies [91, 92] . S. aureus is the most common bacterial skin pathogen affecting HIV-1-infected patients [91] . After the introduction of HAART, there was also a change in the morbidity of cutaneous disorders in HIV-1-infected patients. Calista et al. [93] showed a significant decrease in cutaneous disorders in patients treated with HAART; in particular, there was a reduction in the incidence of cutaneous infections from 301 of 456 patients (66%) not treated with HAART to 266 of 502 patients (53%) treated with HAART, whereas the incidence of adverse cutaneous drug reactions rose from 8% to 20% in those patients treated with HAART. However, it should be noted that staphylococcal infections of the skin remain a frequent and common cutaneous disorder in the era of HAART [91] . Ophthalmic manifestations of AIDS fall into two major categories: vascular disease of the retina and other tissues and OI of the retina and choroid. The most common vascular lesions in HIV-1-infected patients are 'cotton-wool' spots, characteristic manifestations of a diffuse retinal microvasculopathy and retinal ischemia [94] . Infections of the retina and choroid vary in prevalence, but CMV retinitis was the most common intraocular infection in HIV-1-infected patients before the introduction of HAART. In an autopsy study, Morinelli et al. [95] showed that CMV retinitis was by far the most common retinal infection in these patients, with all other infections probably accounting for 5% of retinal infections. The second most common infection of the retina was necrotizing retinitis, caused by varicella-zoster, and the third most common was retinochoroiditis, caused by T. gondii. In a large retrospective study, Jalali et al. [96] observed a dramatic decrease in the incidence of CMV retinitis after the introduction of HAART, representing a 99% reduction since 1993. In addition, much longer remission duration from recurrent CMV retinitis has been reported after the introduction of HAART, and the minimal HIV-1 viral load reached after the initiation of HAART therapy appears to be more important in one retrospective study, because all the studies addressing discontinuation of maintenance therapy were based mainly on CD4 cell count [97] . Thus, the introduction of HAART has had a major impact on the natural history of CMV retinitis, with improved survival time and decreased risk of progression following diagnosis. However, complications of CMV retinitis such as retinal detachment, uveitis and optic atrophy have also frequently occurred in the era of HAART [98] . HIV-1-infected patients are at increased risk of developing malignancies of lymphoid origin. Non-Hodgkin lymphoma (NHL) and HD may occur with increased incidence, as recently reported in a large study including 47 936 HIV-1infected patients [84] . A statistically significant reduction in the incidence of NHL has been demonstrated since the widespread introduction of HAART, although this reduction has not been as dramatic as that for KS [84, 99] . Because most patients respond to HAART with significant increases in CD4 cell counts, at the population level the overall incidence of NHL is expected to decrease [100] . However, it is unclear whether HAART is fully effective at reversing the B-cell stimulation that is associated with HIV-1 infection, and represents a risk factor for NHL. Ratner et al. [101] showed that concomitant use of standard chemotherapy and HAART in HIV-1-infected patients with NHL was effective and safe. In addition, the incidence of all subtypes of NHL has decreased significantly, especially for primary brain lymphoma and immunoblastic lymphoma, in the era of HAART [84] . In a large study, Glaser et al. [102] evaluated 1752 HIV-1-infected patients, and found HD in 13% of the patients diagnosed in the pre-HAART era and in 12% of the patients diagnosed in the post-HAART era. These results confirmed the findings of a previous large prospective study that demonstrated no statistically significant change in the incidence rates for HD in patients treated or not treated with HAART [84] . Advanced HIV-1 infection is associated with an increase in cervical squamous intraepithelial lesions and infection with oncogenic human papillomavirus (HPV) genotypes. Heard et al. [103] demonstrated that HAART may result in a significant reduction of cervical squamous intraepithelial lesions despite the absence of clearance of HPV infection. No study has yet addressed the ultimate effect of HAART on prevention of invasive cervical cancer. Lillo et al. [104] showed that the risk of HPV infection and the risk of squamous intraepithelial lesions both increased with declining CD4 cell count, and there were no differences in terms of persistence of high-risk HPV infection of squamous intraepithelial lesions. A large prospective study demonstrated that there was no substantial change in the incidence of cervical cancer in HIV-1-infected patients treated with HAART [84] . Bacterial infection of the urinary tract is very common in patients with HIV-1 infection. De Gaetano Donati et al. [105] evaluated the effect of HAART on the incidence of bacterial urinary tract infection in HIV-1-infected patients during two periods: before HAART (1992-1995) and after HAART (1997 HAART ( -2000 . They showed a significant reduction in the incidence of bacterial urinary tract infection in these patients, when HAART became the standard therapy. The era of HAART has produced some novel and unexpected developments. Firstly, long-term survival of HIV-1-infected patients treated with HAART has probably led to an increased incidence of mild and moderate HIV-1 encephalopathy (Table 3 ). In a more recent study, Chang et al. [106] suggested that the persistent brain abnormalities in patients with HIV-1 encephalopathy after 3 months of HAART may be a result of reactive inflammatory processes in the brain, and that regimens with two cerebrospinal fluid-penetrating antiretrovirals drugs do not appear to be more effective than those with one cerebrospinal fluidpenetrating drug. Further investigations are needed to better understand the inflammatory processes and molecules involved. HIVAN remains the most common HIV-1-related renal disease in the era of HAART, notwithstanding that antiretroviral drugs have shown beneficial effects on this renal disease (Table 3) . However, additional studies are required to evaluate the risk factors for HIV-1-related renal diseases, in particular population-based studies, studies employing increased use of renal biopsy, and large-scale clinical, prospective and controlled trials to test older and newer antiretroviral drugs. In the future, HIV/HCV coinfection will be a major issue for HIV-1-infected patients treated or not treated with HAART (Table 3) . With the introduction of therapy with pegylated interferon-alpha and ribavirin, the combined treatment approach to HIV/HCV must be investigated in depth to address several concerns, including the timing of the two combined treatments (HAART and pegylated interferon-alpha plus ribavirin), the hepatotoxic effects of HAART in patients with chronic HCV infection, and the pharmacological interactions between antiretroviral agents and anti-HCV drugs. There is a need to further investigate the effects of HAART on certain malignancies, such as invasive cervical cancer and other non-AIDS-defining cancers, including HD, and squamous-cell carcinoma of the head, neck and anus. Mbulaiteye et al. [100] clearly showed that risk of cervical cancer was unrelated to CD4 cell count, and that elevated risks of non-AIDS cancers may be a result of lifestyle factors. Thus, these findings suggest that, in the future, new typical organ-specific manifestations may occur with increased frequency and may contribute to the epidemiology of OIs. Effects of HAART on organ-specific manifestations: the immune reconstitution inflammatory syndrome (IRIS) The IRIS represents a restored capability of the host to mount an inflammatory response against persistent microbial antigens, and leads to the development of symptoms in HIV-1-infected patients with new organ-specific manifestations [6, 106] . The enhancement in the immune response to widely prevalent microorganisms, such as CMV and Mycobacterium spp., appears to be especially marked. Table 4 summarizes the organ-specific manifestations with microorganisms and clinical disorders involved in the IRIS. The vigorous HAART-induced immune response can produce organ-specific lesions in unusual locations, and histological examination of these lesions shows an intense inflammatory response surrounding few, if any, microorganisms [6] . MAC was among the first organisms associated with this syndrome. The presence of granulomas suggests that the clinical manifestation is attributable to a restored inflammatory response. In addition, necrotic subcutaneous nodules, endobronchial tumours, small bowel involvement, and paravertebral abscesses have all been reported as MAC-related unusual lesions [6] . A retrospective review of patients with M. tuberculosis infection treated with HAART found an 8.7% prevalence of paradoxical CNS lesions, including intracranial tuberculoma [107] . There have been cases of mediastinal lymphadenitis, as well as subcutaneous abscesses associated with cryptococcal infection, that occurred months after the institution of HAART (Table 4) . A new type of ocular disease in the setting of CMV infection was recognized after the introduction of HAART, which has been termed 'immune recovery vitreitis'. The inflammatory response can induce proliferative vitreoretinopathy and posterior subcapsular cataracts [108] . Shelburne et al. [6] described a patient who had worsening of cutaneous KS coincident with immune recovery due to HAART. An inflammatory progressive multifocal leuko encephalopathy (PMNL) variant has been reported to have developed in several patients treated with HAART; all the patients who developed PMNL in the setting of immune reconstitution have shown either improvement or at least stability of their neurological deficits [6] . This review summarizes current understanding of organspecific manifestations in HIV-1-infected patients in the HAART era. With the introduction of HAART there has been a striking reduction in the incidence of organ-specific manifestations caused by OIs, such as PCP, cerebral toxoplasmosis, CMV disease, oesophageal candidiasis, and pulmonary and extrapulmonary tuberculosis, and to a lesser extent those manifestations caused by HIV-1 itself, including HIVAN, HIV-1 encephalopathy, and HIV-1related malignancies. We believe it is likely that the widespread use of HAART, particularly the use of newer antiretroviral agents, will produce unique new effects on HIV-1-infected organ systems. Indeed, with increasing recognition of patients with immune reconstitution inflammatory syndrome, characteristic clinical presentations and new organ-specific manifestations have been reported; also, many patients who need HAART are not taking it, and many HIV-1-infected patients are unaware of their infection, and remain at high risk for organ-specific manifestations, including OIs. In our opinion, one of the many challenges in the era of HAART will be to maintain vigilance for OIs, and to monitor new organ-specific manifestations to provide the best possible clinical outcome for HIV-1-infected patients. 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Opportunistic incidence rates from the Tulane/adult spectrum of disease cohort, 1994-98 Conference on Retroviruses and Opportunistic Infections Epidemiology and neuroepidemiology of human immunodeficiency virus infection HIV-related neuropathology, 1985-99: rising prevalence of HIV encephalopathy in the era of highly active antiretroviral therapy Incidence and risk factors for toxoplasmic encephalitis in human immunodeficiency virus-infected patients before and during the highly active antiretroviral therapy Incidence and prevalence of neurological disorders associated with HIV since the introduction of highly active antiretroviral therapy Changes in pathologic findings at autopsy in AIDS cases for the last 15 years Suppression of inflammatory neurotoxins by highly active antiretroviral therapy in human immunodeficiency virus-associated dementia HIV-1 peripheral neuropathy and combination antiretroviral therapy AIDS-related focal brain lesions in the era of highly active antiretroviral therapy Survival of AIDS patients with primary central nervous system lymphoma is dramatically improved by HAART-induced immune recovery The good and evil of HAART in HIV-related progressive multifocal leukoencephalopathy Fatal immune restoration disease in human immunodeficiency virus type 1-infected patients with progressive multifocal leukoencephalopathy: impact of antiretroviral therapy-associated immune reconstitution Impact of HAART on acute psychosis patients with AIDS. XIV International AIDS Conference Heart involvement in AIDS: a prospective study during various stages of the disease Echocardiography detects myocardial damage in AIDS: prospective study in 102 patients Pericardial effusion and pericardiocentesis in human immunodeficiency virus infection Cardiac complications in acquired immunodeficiency syndrome (AIDS): a review Impact of highly active antiretroviral therapy in HIV-positive patients with cardiac involvement Coronary artery disease and human immunodeficiency virus infection HAART and organ-specific manifestations 75 Do protease inhibitors increase the risk for coronary heart disease in patients with HIV-1 infection? Incidence of myocardial infarctions in HIV-infected patients between 1983 and 1998: the Frankfurt HIV-cohort Cardiovascular and cerebovascular events in patients treated for human immunodeficiency virus infection Protease inhibitors and cardiovascular outcomes in patients with HIV-1 Cardiovascular disease risk factors in HIV patients: association with antiretroviral therapy. Results from the DAD study Are we missing an epidemic of HIV-associated nephropathy? USRDS 1998 Annual Data Report. Bethesda, MD, National Institute of Diabetes and Digestive and Kidney Diseases HIV-associated renal disease in London hospitals AIDS-associated membranous nephropathy with advanced renal failure: response to prednisone Renal disease associated with HIV infection: a multicentric study of 60 patients from Paris hospitals Nephropathy associated with human immunodeficiency virus: a report of 11 cases including 6 treated with zidovudine HIV-1-associated nephropathy and response to highly-active antiretroviral therapy Protease inhibitor therapy for HIV infection: the effect of HIVassociated nephrotic syndrome Renal diseases associated with human immunodeficiency virus infection: epidemiology, clinical course, and management Nephropathy in human immunodeficiency virus-1 transgenic mice is due to renal transgene expression Predictors of survival in HIV-infected patients on hemodialysis Effect of HAART on HIV-associated nephropathy (abstract A0882) Renal toxicity of protease inhibitors Tenofovir-related nephrotoxicity in human immunodeficiency virus-infected patients: three cases of renal failure, Fanconi syndrome, and nephrogenic diabetes insipidus The gastrointestinal and hepatic manifestations of the acquired immune deficiency syndrome Diagnosis and treatment of colonic disease in AIDS Declining prevalence of opportunistic gastrointestinal disease in the era of combination antiretroviral therapy Viruses causing diarrhoea in AIDS Campylobacter and Shigella in HIV-seropositive patients Treatment of HIV-1-associated microsporidiosis and cryptosporidiosis with combination antiretroviral therapy Eradication of cryptosporidia and microsporida following successful antiretroviral therapy Patterns and correlates of discontinuation of the intial HAART regimen in an urban outpatient cohort HIV and diarrhea in the era of HAART: 1998 New York State hospitalizations Liver biopsy findings in 501 patients with HIV The effect of concurrent human immunodeficiency virus infection on chronic hepatitis B: a study of 150 homosexual men Natural history of hepatitis C virus infection in multitransfused haemophiliacs: effect of coinfection with human immunodeficiency virus Hepatitis B and C virus coinfection and the risk for hepatoxicity of highly active antiretroviral therapy in HIV-1 infection Reactivation of hepatitis B virus replication accompanied by acute hepatitis in patients receiving highly active antiretroviral therapy Human immunodeficiency virus infection modifies the natural history of chronic parenterally-acquired hepatitis C with an unusually rapid progression to cirrhosis Tritherapy for human immunodeficiency virus infection does not modify replication of hepatitis C virus in coinfected subjects Effects of double and triple antiretroviral agents on the HCV viral load in patients coinfected with HIV and HCV Evolution of coinfection with human immunodeficiency virus and hepatitis C virus in patients with highly active antiretroviral therapy Impact of treatment with human immunodeficiency virus (HIV) protease inhibitors on hepatitis C viremia in patients coinfected with HIV Increase in hepatitis C virus load in haemophiliacs during treatment with highly active antiretroviral therapy Impact of highly active antiretroviral therapy and immunologic status on hepatitis C virus quasispecies diversity in human immunodeficiency virus/ hepatitis C virus-coinfected patients Sustained disappearance of hepatitis C viremia in patients receiving protease inhibitor treatment for human immunodeficiency virus infection Immunologic dynamics in hemophiliac patients infected with hepatitis C virus and human immunodeficiency virus: influence of antiretroviral therapy The changing clinical spectrum of human immunodeficiency virus (HIV)-related oral lesions in 1,000 consecutive patients: a 12-year study in a referral center in Mexico Oral lesions in HIV/AIDS patients undergoing highly active antiretroviral treatment including protease inhibitors: a new face of oral AIDS? AIDS-related malignancies International Collaboration on HIV and Cancer. Highly active antiretroviral therapy and incidence of cancer in human immunodeficiency virus-infected adults Risk of HIV-related Kaposi's sarcoma and non-Hodgkin lymphoma with potent antiretroviral therapy: prospective cohort study Clinical aspects and management of AIDS-related Kaposi's sarcoma HIV protease inhbitors are potent anti-angiogenic molecules and promote regression of Kaposi's sarcoma Changing prevalence of oral manifestations of human immunodeficiency virus in the era of protease inhibitor therapy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod Effect of highly antiretroviral therapy on frequency of oral warts Human papillomavirus-associated oral warts among human immunodeficiency virus-seropositive patients in the era of highly active antiretroviral therapy: an emerging infection Cutaneous manifestations of opportunistic infections in patients with human immunodeficiency virus Dermatological diseases and signs of HIV infection Changing morbidity of cutaneous diseases in patients with HIV after the introduction of highly active antiretroviral therapy including a protease inhibitor HAART and organ-specific manifestations 77 Acquired immune deficiency syndrome: pathogenic mechanisms of ocular disease Opportunistic intraocular infections in AIDS Effect of highly active antiretroviral therapy on the incidence of HIV-related cytomegalovirus retinits and retinal detachment Cytomegalovirus retinitis after initiation of highly active antiretroviral therapy in HIV infected patients: natural history and clinical predictors Changes in the natural history of cytomegalovirus retinitis following the introduction of highly active antiretroviral therapy Identification of herpsevirus-like DNA sequences in AIDS-associated Kaposi's sarcoma Immune deficiency and risk for malignancy among persons with AIDS Chemotherapy for HIV associated non-Hodgkin lymphoma in combination with highly active antiretroviral therapy Population-based patterns of human immunodeficiency virus-related Hodgkin lymphoma in the greater San Francisco bay area, 1988-98 Early regression of cervical lesions in HIV-seropositive women receiving highly active antiretroviral therapy HPV infection and associated cervical disease in HIV infected women: effect on highly active antiretroviral therapy Impact of highly active antiretroviral therapy (HAART) on the incidence of bacterial infections in HIV-1 infected subjects Persistent brain abnormalities in antiretroviral-naïve HIV patients 3 months after HAART Miliary tuberculosis with paradoxical expansion of intracranial tuberculomas complicating immunodeficiency virus infection in a patient receiving highly active antiretroviral therapy Neovascularization of the optic disc after highly active antiretroviral therapy in an AIDS patient with cytomegalovirus retinitis. A new immune recovery-related ocular disorder?