key: cord-0727818-l6f6auq4
authors: Trubitsin, B. V.; Milanovsky, G. E.; Mamedov, M. D.; Semenov, A. Yu.; Tikhonov, A. N.
title: The Interaction of Water-Soluble Nitroxide Radicals with Photosystem II
date: 2021-09-09
journal: Appl Magn Reson
DOI: 10.1007/s00723-021-01425-z
sha: 275da00e16c997b0461ef47c62048e7f7327805a
doc_id: 727818
cord_uid: l6f6auq4

In this work, we investigated the redox transients of a number of water-soluble spin labels upon their interactions with Photosystem II (PS II) core complexes isolated from spinach leaves. We have found that the reactivity of nitroxide radicals, determined by the rate of their reduction upon illumination of PS II, depends on the chemical structure of radicals and the capability of their coming close to low-potential redox centers of photoactive PS II complexes. An enhanced capability of nitroxide radicals to accept electrons from PS II correlates with their chemical structure. Nitroxide radicals NTI (2,2,5,5-tetramethyl-4-nitromethylene-3-imidazolidine-N-oxyl) and Tacet (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl-acetate), containing polar groups, appear to be most efficient acceptors of electrons donated by PS II compared to neutral (TEMPOL, 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl) or positively charged (Tamine, 4-amino-2,2,6,6-tetramethylpiperidine-l-oxyl) spin labels. We assume that enhanced reactivities of polar nitroxide radicals, NTI and Tacet, are determined (1) by their relatively high redox potentials, providing the possibility to accept electrons from PS II, and (2) by their affinities to the closest binding sites on the surface of PS II in the vicinity of the primary plastoquinone acceptor PQ(A) (12–14 Å) or/and in the intraprotein cavity for the secondary plastoquinone PQ(B) (~ 22 Å).

Water-soluble nitroxide radicals have found numerous applications in chemistry, biology, and medicine. The most interesting applications of nitroxide radicals in biology and medicine are associated with the development of drugs [1] . For example, recently the stable nitroxide 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL) has been suggested as the reagent that can cause the oxidation of the Fe-S clusters of the RNA-dependent RNA polymerase (RdRp). These clusters serve as cofactors for the SARS-CoV-2 RdRp. The inhibition of the RdRp by TEMPOL and/or by related stable nitroxide radicals, blocking SARS-CoV-2 replication in cell culture, might be used for potential COVID-19 therapies during active viral infection [2] .

In the current work, we have focused on applications of water-soluble nitroxide radicals for the study of electron transport processes in oxygenic photosynthesis. Photosynthetic apparatus of oxygenic organisms (cyanobacteria, alga, and higher plants) includes the pigment-protein complex of photosystem II (PS II), which is the light-driven water-plastoquinone oxidoreductase located in the thylakoid membranes. The structure of the dimeric form of PS II in thermophilic cyanobacteria was determined by X-ray analysis with the atomic resolution of 1.9 Å [3] . Each monomer of the PS II complex contains chlorophyll (Chl) dimer P 680 , monomeric Chl molecules Chl D1 and Chl D2 , pheophytin molecules Phe A and Phe B , the primary (PQ A ) and the secondary (PQ B ) plastoquinone molecules, redox-active tyrosine Y Z and the water-oxidizing complex (WOC). The WOC, consisting of the inorganic Mn 4 CaO 5 cluster and surrounding protein matrix, is oriented towards the luminal side of the thylakoid membrane [3] [4] [5] [6] [7] . The energy of light quanta absorbed by the light-harvesting antenna migrates to the reaction center, where the charge separation occurs, forming the radical pair P 680 + Phe A -. Reduced pheophytin donates an electron to the tightly bound PQ A , which passes the electron further to PQ B . After the consecutive two-electron reduction of PQ B by PQ A and its protonation (PQ B + 2e − + 2H + → PQ B H 2 ), the fully reduced plastoquinone dissociates into the lipid phase of the thylakoid membrane in exchange for the PQ molecule from the PQ/PQH 2 pool. The oxidized primary donor P 680 + is reduced by Y Z . The tyrosine Y Z links P 680 with the WOC (reviewed in [5] [6] [7] ).

The light-induced charge transfer in PS II is coupled to the generation of the transmembrane electric potential difference [8, 9] . The search for organic and inorganic compounds capable of efficient accepting electrons from the acceptor side of PS II is relevant to better understanding of molecular mechanisms of the PS II functioning. This may be of practical application to create highly effective and ecologically friendly solar-electric energy converters [10] [11] [12] . In PS II, both PQ A and PQ B molecules are localized at a distance of ~ 10 Å from the protein-water boundary [3, 4] . It is conceivably, therefore, that they may transfer electrons to exogenous electron acceptors. Some of these acceptors may also serve as effective electron transfer intermediates between PS II and semiconductor electrodes [13] . In the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU)-an inhibitor of electron transfer from PQ A to PQ B , PS II can donate electrons directly from PQ A − to the oxidized form of cytochrome (cyt) c or the synthetic cationic complex Co(III). The commonly used mediators between PS II and the electrodes include 2,6-dichloro-p-benzoquinone, 2,6-dimethyl-p-benzoquinone, phenyl-p-benzoquinone [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] and the oxidized cyt c [24, 25] . The other potential intermediate electron acceptors from PQ A − to semiconductors are paramagnetic molecules (spin labels) interacting with the acceptor side of PS II. The light-induced electron transfer from PS II to spin labels (nitroxide radicals) can be monitored by measuring the changes in the intensity of their electron paramagnetic resonance (EPR) signals [26] .

Water-soluble nitroxide radicals have found numerous applications in chemistry and biology (for references, see [27] ). In particular, the imidazoline-and imidazolidine-based nitroxides are widely used as molecular probes for measuring the proton activity inside the thylakoid lumen (for review, see [28] ). EPR spectra of pH-sensitive spin labels have distinct features that allow to distinguish between the signals given by other paramagnetic species. In our earlier studies, we investigated the interaction of a number of water-soluble imidazoline-and imidazolidine-based nitroxide radicals with isolated chloroplasts [29] . It was demonstrated that the light-induced changes in the EPR signals of nitroxide radicals are determined by several factors: (1) the ΔpH-driven accumulation of radicals inside the thylakoids and concomitant changes in the shape of their EPR spectra, (2) the irreversible loss of paramagnetism due to spin label interactions with the chloroplast electron transport chain, and (3) specific effects caused by changes in the partial pressure of molecular oxygen (O 2 ) in the result of the light-induced evolution (or consumption) of paramagnetic molecules 3 O 2 . The loss of paramagnetism (the "reactivity" of spin labels) may cause the problems in their applications, e.g., for probing structural changes in biological systems and physical-chemical properties of spin label surroundings. This property of nitroxide radicals can be used, however, for the analysis of redox activity of biological systems. Having spin labels capable of redox transformations, one could use them as the indicators of electron transport in bioenergetic processes [27] [28] [29] .

The main purpose of this work is to investigate redox transients of a number of water-soluble spin labels that can occur due to their interactions with isolated PS II. We have found that the reactivity of nitroxide radicals, determined by the rate of their reduction upon illumination of PS II, depends on the chemical structure of radicals and the capability of their approaching to PQ A molecules on the acceptor side of PS II.

Spinach PS II core complexes were isolated from commercial spinach plants (Spinacia oleracea) as described in [30] by treatment of the membrane fragments with dodecyl-β-d-maltoside (DM, 10:1, detergent/Chl) for 1 h followed by sucrose density centrifugation (20-40%) (Beckman VTi 50 vertical rotor) for 7 h at 4 °C at 210,000 g.

A stock suspension of PS II core particles in 25 mM Mes-NaOH buffer (pH 6.5), containing 0.33 M sucrose, 15 mM NaCl, 10 mM CaCl 2 and 0.03% (w/v) DM, was frozen in liquid nitrogen as small aliquots with a Chla concentration of 2.5 mg mL −1 and stored at − 80 °C.

To prepare manganese-depleted apo-WOC-PSII complexes, thawed intact PS II particles (0.5 mg Chl per ml) were incubated in the presence of 0.8 M Tris-HCl buffer (pH 8.3) for 30 min at 23 °C, followed by 3 times washing with the buffer (50 mM Mes-NaOH, pH 6.5) [31] .

Final concentrations of P 680 centers in the samples used for EPR measurements were 10 μM for PS II and 25 μM for apo-WOC-PSII, respectively. PS II and apo-WOC-PSII complexes were suspended in 30 mM Mes-NaOH (pH 7.5).

Chemical structures of nitroxide radicals and their brief names used in this work are shown in Fig. 1 . Spin labels 4-amino-2,2,6,6-tetramethylpiperidine-l-oxyl (Tamine) and 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL) were purchased from Sigma (USA). Spin labels 4-amino-2,2,5,5-tetramethyl-3-imidazoline-1-oxyl (ATI) and 2,2,5,5-tetramethyl-4-nitromethylene-3-imidazolidine-N-oxyl (NTI) were synthesized by I.A. Grigor'ev and I.A. Kirilyuk (Institute of Organic Chemistry, Novosibirsk) as described in [29] . Spin label 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl-acetate (TEMPOLacetate or Tacet) was a gift of professor E.K. Ruuge (Faculty of Physics, Moscow State University).

Concentrations of spin labels in the suspensions of PS II and apo-WOC-PSII used for EPR measurements were about 30 times higher than the concentrations of P 680 in the samples, i.e., 300 and 750 μM, respectively. 

The conventional first-derivative EPR spectra (first harmonic absorption in phase, 100 kHz field modulation) were measured with a Varian E-4 X-band spectrometer equipped with a variable temperature controller [32] . EPR spectra were recorded at a modulation amplitude of 0.5G and microwave power 1, 10, or 50 mW, as indicated. Samples were placed into a thin-walled gas-permeable capillaries made of optically transparent methylpentane polymer (TFE tubes from Zeus Inc.; internal diameter, 0.635 mm; wall thickness, 0.051 mm) as described in [29] . Measurements were performed at room temperature (~ 20-22 °C). Samples were illuminated by white light from a 100 W tungsten lamp (photon flux about 10 µEinstein m −2 s −1 at the sample surface); infrared light was cut-off with a 5 cm layer of water. The control experiments showed that illumination of water suspensions of spin labels (without PS II complexes) did not cause changes in the EPR spectra that might be caused by the light-induced destruction of the nitroxides.

Potential sites of spin label binding on the surface of PS II have been found with Autodock Vina software [33] . The docking sites were visualized and analyzed with VMD molecular viewer [34] .

In this section, we describe the results of the study of spin label interactions with intact core PS II complexes and the samples depleted of water-oxidizing complex (apo-WOC-PSII). In the case of apo-WOC-PSII, electron transport through PS II was supported by the addition of 10 mM NH 2 OH. The following nitroxide radicals were used ( Fig. 1) : ATI (pK a = 6.2), NTI (pK a = 8.0), TEMPOamine (Tamine, pK a = 8.9), TEMPOL, and TEMPOacetate (Tacet). All the radicals dissolved in aqueous solutions give triplet EPR signals with narrow lines, typical of nitroxide radicals rapidly tumbling in the water bulk phase (see, for example, the EPR spectrum of Tamine shown in Fig. 2a) .

The light-induced changes in the amplitude of the EPR signals were monitored by measuring the amplitudes of the low-field components of the EPR spectrum (Fig. 2) . This choice is based on the fact that the low-field line is less sensitive to distortions caused by anisotropy of radical rotations. The response of water-soluble spin labels to illumination of PS II can be caused, in general, by different effects, including (i) the redox transients of nitroxide radical and (ii) the influence of paramagnetic 3 O 2 molecules on relaxation properties of radicals. We can discern these effects by recording the EPR signals at different levels of microwave power. Figure 2b shows the light-induced changes in the amplitude of the low-field lines of the EPR signals of Tacet and Tamine recorded at 1 and 50 mW. As one can see, the light-induced response of the signal amplitude depends on the structure of the spin label and the microwave power level. For Tacet recorded at 1 mW, the amplitude of the EPR signal gradually decreases after switching the light on. The decrease in the signal is caused by Tacet interaction (reduction or oxidation) with PS II complexes. In contrast to Tacet, the EPR signal of Tamine remains at the same level regardless of illumination. This indicates that Tamine is unable to interact with PS II. We assume that such a difference is accounted for by different availabilities of Tacet and Tamine to electron carriers of PS II.

When the EPR signals were recorded at microwave power 50 mW, we observed an insignificant initial increase in the amplitudes of the EPR lines of both radicals, Tacet and Tamine. This effect can be explained by the light-induced evolution of O 2 dring illumination of PS II. Molecular oxygen is paramagnetic species ( 3 O 2 , spin S = 1), which can influence the relaxation properties of nitroxide radicals [35] [36] [37] [38] [39] [40] . The O 2 -dependent modulation of the signal amplitude is associated with the action of 3 O 2 as a paramagnetic "relaxator". It is important to note that the sign of the effect caused by 3 O 2 depends on microwave power upon measuring the EPR signals of nitroxide radicals. Comparing the EPR signals of nitroxides recorded at low and high levels of microwave power (P), an experimenter can discriminate between the effects of O 2 as the "relaxator" from other factors that can influence the EPR spectra (e.g., the loss of paramagnetism caused by redox transients [29] ). At low microwave power (P < 1-5 mW), when the EPR signals of nitroxides are not saturated, increasing with the rise of P, 3 O 2 molecules reveal themselves as a line-broadening agent. In this case, the interaction of 3 O 2 with nitroxide radicals leads to broadening the EPR signal, accompanied by a decrease in the signal amplitude. Otherwise, at sufficiently high microwave power (P > 50 mW), molecular oxygen causes an opposite effect: 3 O 2 induces an increase in the signal amplitude [29] . This is because at high microwave power the rate of thermal relaxation of nitroxide radicals to the ground state is not sufficient to establish thermal equilibrium. In the meantime, paramagnetic 3 O 2 molecules accelerate the spin-lattice relaxation of nitroxide radicals, thereby releasing the broadening of the EPR signal at high microwave power. Thus, 3 O 2 attenuates the broadening of the radical signals at high microwave power, leading to an increase in the signal amplitude [24, 29, [36] [37] [38] [39] [40] . Comparing the timecourses of the light-induced changes in the EPR signal amplitude at P = 1 mW and P = 50 mW (Fig. 2) , we observed the initial increase in the EPR line amplitudes at 50 mW; however, this effect was not observed at 1 mW. This demonstrates that the native PS II samples used in our experiments were photochemically active, producing molecular oxygen upon their illumination. Note that de-oxygenation of samples placed inside the gas-permeable TFE tubes by 10-min blowing with N 2 gas eliminated the oxygen effect (data not shown). Figure 3 shows the light-induced changes in the EPR signals of four different spin labels in suspensions of apo-WOC-PSII. In these cases, electron flow to the primary plastoquinone PQ A was supported due to electrons donated to P 680 + (via Y z ) by NH 2 OH molecules. All the measurements with the apo-WOC-PSII complexes have been performed on de-oxygenated suspensions. This precluded the potential effects of the interactions of molecular oxygen with spin labels that might interfere with the redox transformations of nitroxide radicals. As noted above, paramagnetic molecules 3 O 2 may influence the relaxation parameters of nitroxide radicals that could reveal themselves in the time-course of the light-induced changes in the EPR signal. Also, we cannot exclude that superoxide radicals formed upon one-electron reduction Fig. 3 Kinetics of the lightinduced changes in the amplitude of the low-field lines of the EPR signals of spin labels TEMPOL, Tamine, Tacet, and NTI in de-oxygenated solutions of apo-WOC-PSII; microwave power 10 mW of O 2 molecules may act as the oxidant which would re-oxidize the > N-OH fragments of spin labels. Comparing the interaction of different spin labels with apo-WOC-PSII complexes, we have found that the rate of the light-induced reduction of EPR signal increases in the following sequence: TEMPOL < Tamine < Tacet < NTI. TEMPOL and Tamine exhibit lower reactivity. Similar to intact PS II complexes, apo-WOC-PSII reduces Tacet more rapidly than Tamine. Note that NTI, which contains the polar group > NO 2 , revealed much more rapid reduction than Tacet. Figure 4 depicts a general scheme of spin label interactions with PS II. The light-induced loss of the nitroxide radical (> N-O · ) paramagnetism may proceed, in principle, either due the radical > N-O · reduction to > N-OH or as the result of > N-O · oxidation to > N + = O. Considering the line of arguments pro and contra these points, we can appeal, first of all, to structural data (see Figs. 5 and 6, and explanations in the text below). The architecture of the PS II complex suggests that the light-induced loss of paramagnetism of water-soluble nitroxide radicals occurs predominantly due to electron donation to nitroxides by electron carriers located on the acceptor side of PS II, most likely via PQ A -. We can exclude PQ B as a potential donor of electrons for spin labels, because in isolated core complexes of PS II the binding sites of PQ B were unoccupied. Concerning a hypothetical possibility of the light-induced oxidation of spin labels, we must note that the high-potential redox cofactors (oxidized centers P 680 + and/or oxidized redox species in WOC) might be considered as the potential recipients of electrons from nitroxide radicals. However, we can exclude P 680 + centers as direct acceptors of electrons from spin labels, because P 680 is deeply buried into the core of the PS II protein complex. In WOC, high-potential redox intermediates are also sequestered from the water-soluble nitroxides. Thus, we conclude that it is the reduction of the nitroxide radicals by PQ A that is responsible predominantly for the light-induced loss of spin label paramagnetism. Fig. 4 The potential pathways of the nitroxide radical interactions with PS II Note that PS II complexes devoid of the Mn 4 CaO 5 cluster can reduce the EPR signals of spin labels during illumination (Fig. 3) . In this case, however, the rates of the signal decay were lower than in the intact PS II complexes. We assume that this might be caused by two reasons, (1) the changes in the midpoint redox potential of the redox pair PQ A − /PQ A in the apo-WOC-PSII, and (2) kinetic effects. The redox potential of the PQ A − /PQ A pair has been a subject of numerous researches and heated debates for decades [41] [42] [43] [44] [45] [46] [47] [48] [49] [50] . Recently, this point was critically analyzed in view of the data from the Rutherford's laboratory. According to [41, 42, 46] , the primary plastoquinone PQ A in the apo-WOC-PSII is characterized by higher (about 100-150 mV) redox potential of PQ A than in the native PS II (E m (PQ A − /PQ A ) = − 144 ± 2 mV). The bicarbonate ligation to the non-heme iron markedly increased the redox potential E m of PQ A in both the native and the apo-WOC-PSII complexes [46] . Shibamoto et al. [43] , confirmed the 150-mV redox difference with and without the Mn cluster. Thus, one might expect that the apo-WOC-PSII complexes will be of lower reducing power as compared to intact preparations of PS II, and this factor could slow down the loss of the radical EPR signal in the apo-WOC-PSII system.

Also, it is highly likely that there may be a kinetic effect, which reveals itself when the WOC is removed (apo-WOC-PSII) and the electron transfer from Y Z to P 680 + slows down [4, 6, 7] . It is well known that the properties of Y Z in the apo-WOC-PSII differ dramatically from those in native PS II [4] . Although the light-induced electron flow through P 680 can be maintained due to the presence of artificial electron donors, like NH 2 OH, the rate at which NH 2 OH in solution rereduces Y Z must be much slower than in intact PS II complexes. That is why the apo-WOC-PSII complexes revealed slower rates of nitroxide reduction as compared to the native PS II ( Table 1) . Results of the comparative study of the reduction of different nitroxide radicals by PS II are summarized in Table 1 . Note that the reduction of different spin labels by apo-WOC-PSII revealed a similar regularity as in intact PS II complexes. We have found that the most significant rate of radical reduction was observed for spin labels NTI and Tacet, which contain the polar groups. Otherwise, electrically neutral spin labels TEMPOL and ATI (pK a = 6.2, ATI is deprotonated at pH 7.5), and positively charged Tamine (pK a = 8.9) showed lower rates of reduction then NTI and Tacet.

Taking together the above results, we assume that the basic reasons for different redox activities of the nitroxide radicals examined in this work are (1) various affinities (docking) of spin labels to PS II and (2) the differences in the reduction potentials of nitroxide radicals. To analyze the role of the first factor, we consider below the potential sites of spin label binding to PS II complex and their positions towards PQ A . There are good reasons to believe that the reduced plastoquinone PQ A serves as the immediate electron donor for nitroxide radicals. The redox potential of the PQ A -/PQ A pair (E m ~ − 100 mV) is appropriate for the reduction of nitroxide radicals, and this process could be realized if the distance between the redox groups of PQ A and spin labels is sufficiently short to provide the electron transfer. In the context of electron transfer from PQ A to water-soluble nitroxides, we have also to mention a potential influence of the non-heme iron (NHI) on the rate of this reaction. The role of the NHI in PS II in general is not well understood [3-5, 48, 49] . Presumably, the NHI may serve as regulator of electron transport on the acceptor side of PS II, which influences the rate of electron transfer from PQ A to PQ B [48, 49, [51] [52] [53] . We might suggest that the NHI could also act in a similar fashion, accelerating electron transfer from PQ A − to nitroxide radicals. The colored balls designate the selected positions of different spin labels determined by the docking method. We can see that different spin labels are clustered approximately in the areas, collectively designated here as sites 1, 2, and 3. The energies of spin label binding to these sites have been estimated as ΔE ≈ 6.0-6.5 kcal/mole. Characteristic distances between the redox moieties of PQ A and spin labels positioned in sites 1, 2 and 3 are about 12, 14 and 22 Å, respectively (Fig. 6) . According to the semi-empirical Dutton's ruler [54] , these distances fall in the interval that may ensure the electron transfer tunneling between PQ A and the redox centers of spin ) and acceptor (the nitroxide group). Binding sites 1 and 2 have positively charged amino acids (Arg28T and Arg251D in the site 1; Arg28T and Lys227A in the site 2); site 2 also contains negatively charged Glu25T. The rest of the site 2 surface is comprised of uncharged polar and nonpolar residues, including: Ser471C, Asn250D, Ile29T, Leu467C and Pro470C in site 1, Ser262D, Thr30T, Tyr224A, Pro26T and lipid molecules in site 2. Site 3 is comprised entirely of uncharged and mostly nonpolar residues, and lipids [3, 4] . The abundance of uncharged residues allows to suggest that the spin label binding to all three sites is governed by hydrophobic interactions. Positive charge of sites 1 and 2 pose obstacles for positively charged spin labels to bind these sites.

Comparing the reaction activities of spin labels investigated in this work, we have found that NTI and Tacet, which contain polar groups, reveal relatively high rates of the nitroxide radical reduction by PS II. There are good reasons to believe that electron density in the radical fragment of NTI is reduced as compared to other spin labels. In NTI molecule, the NO 2 group contains the positively charged N atom (Fig. 7a) , which can attract electron density from the paramagnetic > N-O · Fig. 7 EPR spectra of spin labels NTI and ATI in water. EPR spectra of deprotonated and protonated forms of ATI were obtained for ATI solutions at pH 5 and pH 8 fragment, thereby increasing its electrophilicity. A decrease in electron density on the N atom of the > N-O · group reveals itself as a decrease in the hyperfine splitting constant a iso of the NTI molecule. In the bottom panel of Fig. 7 , we compare the EPR spectra of NTI and ATI molecules. Note that the NTI and deprotonated ATI molecules show different values of the splitting parameter a iso . Deprotonated (electrically neutral) ATI molecules reveal higher value of a iso than protonated (positively charged) ATI(H + ) molecules. The protonation of ATI causes a marked decrease in a iso (Fig. 7 , for more details, see [55, 56] ). This observation supports the notion that the positive charge in the moiety of the spin label molecule reduces electron density in the radical fragment > N−O · . Interestingly, NTI and protonated ATI molecules reveal close values of the EPR spectrum parameter a iso . These data suggest that changes in the structure of spin labels causes the redistribution of the electron and spin densities over the spin label molecule. A decrease in the electron density of the radical fragment > N−O · might enhance the electrophilicity of this group, thereby increasing the redox potential of the > N−O · / > N− OH redox pair (for references, see [27] ). This can explain an enhanced capacity of NTI as the recipient of an electron donated by PS II.

In this work, we have found that the redox activity of water-soluble nitroxide radicals depends on their chemical structure. Nitroxide radicals NTI (2,2,5,5-tetramethyl-4-nitromethylene-3-imidazolidine-N-oxyl) and Tacet (4-hydroxy-2,2,6,6tetramethylpiperidine-1-oxyl-acetate) with the polar groups appear to be most efficient acceptors of electrons donated by PS II as compared to neutral or positively charged spin labels (TEMPOL and Tamine). We assume that enhanced reactivities of NTI and Tacet are determined by their relatively high redox potentials and their affinities to the binding sites on the surface (and/or in the PQ B -binding pocket) of the PS II protein complex located at distances ~ 12-22 Å from the redox group of the primary plastoquinone PQ A .

We dedicate this article to our dear and long-term collaborators, Klaus Moebius and Kev Salikhov, on the occasion of their glorious anniversaries.

Biomed. EPR, Part A Free Radicals

Nitroxide Spin Labels: Reactions in Biology and Chemistry

Spin Exchange Principles and Applications in Chemistry and Biology

Mechanism of proton-coupled quinone reduction in Photosystem II

Photosystem II: the light-driven water: plastoquinone oxidoreductase

We thank professors E.K. Ruuge and I.A. Grigor'ev, and Dr. I.A. Kirilyuk for the gifts of spin labels Tacet, ATI and NTI. The authors acknowledge partial support from Lomonosov Moscow State University Program of Development.

The authors declare that they have no conflict of interest.