key: cord-0727435-zstfdmkl
authors: Abravanel, Florence; Miédouge, Marcel; Chapuy-Regaud, Sabine; Mansuy, Jean-Michel; Izopet, Jacques
title: Clinical performance of a rapid test compared to a microplate test to detect total anti SARS-CoV-2 antibodies directed to the spike protein
date: 2020-06-29
journal: J Clin Virol
DOI: 10.1016/j.jcv.2020.104528
sha: b9b620434baf2cbc860ac44604777cda3fecd1bf
doc_id: 727435
cord_uid: zstfdmkl

nan

1

Florence Abravanel 1,2,* , Marcel Miédouge 2 , Sabine Chapuy-Regaud 1,2 , Jean-Michel Mansuy 2 and Jacques Izopet 1 The recent emergence of the SARS-CoV-2 pandemic has posed formidable challenges for clinical laboratories. While immunoassays are already available, their diagnostic accuracy and optimal use remain undefined. Serologic tests could be used as complements to assays for virus nucleic acid in the diagnosis of COVID-19 (1-3). Serologic assays that accurately assess prior infection and immunity to SARS-CoV-2 are essential for retrospective diagnosis, epidemiologic studies, ongoing surveillance and vaccine studies (1, 4) .

We have evaluated 2 serological assays that detect total anti-SARS-CoV-2 antibodies. One is a rapid immunochromatographic test (SARS-CoV-2 Ab Rapid Test, Beijing Wantai Biological Pharmacy We therefore find that the Wantai rapid test and the microplate assay have excellent specificity but the rapid test appears to be less sensitive than the microplate assay. Although the rapid test could be ideal for point-of-care use because it requires no highly skilled personnel, no batch testing with a result in less than 15 minutes, our evidence indicates that the diagnostic performance of the two assays may differ in the early stages of infection and for asymptomatic patients. Despite small sample size, J o u r n a l P r e -p r o o f our data could be useful for defining the practical application of these assays that detect anti-SARS-CoV-2 antibodies.

Declarations of Competing Interest: None.

Funding: None.

Diagnostic Testing for Severe Acute Respiratory Syndrome-Related Coronavirus 2: A Narrative Review

Laboratory Diagnosis of COVID-19: Current Issues and Challenges

Antibody Detection and Dynamic Characteristics in Patients with COVID-19

The Role of Antibody Testing for SARS-CoV-2: Is There One?

Performance of SARS-CoV-2 antibody assays in different stages of the infection: Comparison of commercial ELISA and rapid tests

The English text was edited by Dr Owen Parkes.