key: cord-0725909-mc3u86gg
authors: Meisner, Julianne; Roberts, D Allen; Rodriguez, Patricia; Sharma, Monisha; Newman Owiredu, Morkor; Gomez, Bertha; de Mello, Maeve B; Bobrik, Alexey; Vodianyk, Arkadii; Storey, Andrew; Githuka, George; Chidarikire, Thato; Barnabas, Ruanne; Farid, Shiza; Essajee, Shaffiq; Jamil, Muhammad S; Baggaley, Rachel; Johnson, Cheryl; Drake, Alison L
title: Optimizing HIV retesting during pregnancy and postpartum in four countries: a cost‐effectiveness analysis
date: 2021-03-31
journal: J Int AIDS Soc
DOI: 10.1002/jia2.25686
sha: 3547eb0d32b12e031006dd30898a16394f89b1ee
doc_id: 725909
cord_uid: mc3u86gg

INTRODUCTION: HIV retesting during late pregnancy and breastfeeding can help detect new maternal infections and prevent mother‐to‐child HIV transmission (MTCT), but the optimal timing and cost‐effectiveness of maternal retesting remain uncertain. METHODS: We constructed deterministic models to assess the health and economic impact of maternal HIV retesting on a hypothetical population of pregnant women, following initial testing in pregnancy, on MTCT in four countries: South Africa and Kenya (high/intermediate HIV prevalence), and Colombia and Ukraine (low HIV prevalence). We evaluated six scenarios with varying retesting frequencies from late in antenatal care (ANC) through nine months postpartum. We compared strategies using incremental cost‐effectiveness ratios (ICERs) over a 20‐year time horizon using country‐specific thresholds. RESULTS: We found maternal retesting once in late ANC with catch‐up testing through six weeks postpartum was cost‐effective in Kenya (ICER = $166 per DALY averted) and South Africa (ICER=$289 per DALY averted). This strategy prevented 19% (Kenya) and 12% (South Africa) of infant HIV infections. Adding one or two additional retests postpartum provided smaller benefits (1 to 2 percentage point increase in infections averted versus one retest). Adding three retests during the postpartum period averted additional infections (1 to 3 percentage point increase in infections averted versus one retest) but ICERs ($7639 and in Kenya and $11 985 in South Africa) greatly exceeded the cost‐effectiveness thresholds. In Colombia and Ukraine, all retesting strategies exceeded the cost‐effectiveness threshold and prevented few infant infections (up to 31 and 5 infections, respectively). CONCLUSIONS: In high HIV burden settings with MTCT rates similar to those seen in Kenya and South Africa, HIV retesting once in late ANC, with subsequent intervention, is the most cost‐effective strategy for preventing infant HIV infections. In these settings, two HIV retests postpartum marginally reduced MTCT and were less costly than adding three retests. Retesting in low‐burden settings with MTCT rates similar to Colombia and Ukraine was not cost‐effective at any time point due to very low HIV prevalence and limited breastfeeding.

and treating maternal HIV infections is vital to achieving the UNAIDS 95-95-95 fast-track targets for achieving low HIV incidence by 2030 [6] .

Universal HIV testing for pregnant women has been found to be highly cost-effective, even in very low HIV prevalence settings [7] , and retesting during pregnancy and postpartum is a promising approach to reduce MTCT risk. The World Health Organization (WHO) has recommended retesting in the third trimester or during labour/delivery in high HIV prevalence settings since 2006, and retesting during breastfeeding since 2015 [8, 9] . Despite the promise of maternal HIV retesting, health and economic impacts and optimal timing and frequency are not well-characterized [7, 10] . Modelling analyses in Uganda, South Africa and India have found retesting in late pregnancy, at delivery, or very early in the postpartum period to be cost-effective [11] [12] [13] . However, recent scale-up of ART coverage and HIV prevention interventions limit the relevance of these analyses, and in light of evidence that women in high burden settings have high HIV risk throughout the postpartum period [14, 15] , postpartum retesting warrants investigation.

The HIV funding gap is projected to increase to US$7.2 billion below that needed to achieve 2030 fast-track targets [16] . As countries strive to achieve the elimination of MTCT (EMTCT), policymakers must allocate limited resources efficiently. To provide guidance to countries deciding whether to implement maternal retesting, and if so the optimal frequency and time(s) to retest, we assessed the cost-effectiveness of various strategies of HIV retesting during pregnancy, labour/ delivery and postpartum in four countries representing a range of national HIV prevalence.

We constructed a deterministic state-transition model using Excel (Microsoft Corporation, Redmond, WA, USA) to reflect HIV disease progression, HIV testing and treatment during pregnancy and postpartum (54 total states: 6 maternal HIV stages stratified on 9 antenatal and postpartum periods) (Appendices S1 and S2). We chose to model four countries (South Africa, Kenya, Colombia and Ukraine) with varying geography and HIV prevalence and adequate data availability. Countries were classified based on WHO criteria as having high (>15%, South Africa), intermediate (5% to 15%, Kenya) and low national HIV prevalence (<5%, Colombia and Ukraine) [7] . We modelled the annual number of pregnant women in each country (or number of births as a proxy) and used weekly time steps from the start of pregnancy to 12 months postpartum. Infant disability-adjusted life years (DALYs) and treatment costs were estimated over a 20-year time horizon. We did not model infants explicitly; all cost and health events occurring after infant infection were estimated from model outputs. We evaluated six retesting scenarios varying the number and timing of retests (Table 1) . We selected retest timing based on routine maternal and child health (MCH) visits/immunization schedules and country policies [10] . Current guidelines for retesting are as follows: South Africaeach ANC visit, labour and delivery and every three months while breastfeeding [17]; Kenyathird trimester, labour and delivery, six weeks and six months postpartum [18]; Ukraineduring pregnancy and labour and delivery only if high risk or unknown status [19] ; Colombiasecond and third trimester and labour and delivery [20] . We defined "late ANC" based on each country's ANC visit schedule, median gestational age at ANC initiation and recommended HIV retesting interval. Late ANC corresponds to a gestational age in the third trimester in Kenya and South Africa and late second trimester in Colombia and Ukraine (Table 2a) .

Model parameters (Table 2a; Appendix S3) were derived from published literature (through April 2020), expert opinion, or assumptions if data were not available. As our model simulates a hypothetical population not corresponding to a specific year, for all parameters we used the most up-to-date value available. HIV testing in our model requires visit attendance, tests in stock, test acceptance and receipt of results. In the base case scenario, HIV testing is offered only once at the first ANC, which is assumed to occur at a country-specific gestational age. To reflect a setting in which retesting at delivery or six weeks postpartum would only occur if women had not retested previously, we modelled catch-up retesting at delivery to be conditional on the probability of not retesting at late pregnancy, and retesting at six weeks postpartum to be conditional on the probability of not retesting in late ANC or delivery.

We assumed no retesting between visits, no viral suppression during recent (acute) infection, no repeat or multiple pregnancies and no foetal losses. We assumed all women diagnosed HIV-positive were ART eligible [21] and based ART initiation and viral suppression rates on country-specific estimates. We assumed a constant weekly risk of ART discontinuation resulting in 74% retention by one year postpartum [22] . MTCT depended on pregnancy/postpartum period; maternal disease stage, use of ART and viral load suppression; infant use of antiretroviral prophylaxis (ARVs); and breastfeeding practices (Appendix S3). Infant HIV testing was only reflected in the probability an infected infant received ART, 

Cells containing a U denote a visit where retesting is offered. ANC, antenatal care visit. Catch-up testing refers to testing at delivery for those who did not test in late ANC, and testing at six weeks postpartum for those who did not test at late ANC or delivery. 

Modelled health outcomes included infant HIV infections, deaths and DALYs. In addition to percent of total infant infections averted by each scenario, we calculated the percent of maximum possible infant infections averted relative to no retesting by excluding infant infections from women diagnosed with HIV prior to pregnancy, as these women would neither be eligible for nor benefit from additional HIV testing.

We estimated costs from a healthcare system perspective, including costs for maternal retesting, maternal ART, infant ARV prophylaxis and infant ART (Table 2b, Appendix S2) .

Costs and resource utilization were obtained from the published literature, a time-motion study in Kenya (Appendix S5), in-country experts, or assumptions (Table 2b , Appendix S2). Unit costs were applied to testing and treatment/prophylaxis as women transitioned though model states. Costs were adjusted to 2017 USD [26] . Weekly maternal ART costs were applied to women receiving ART and modelled through 12 months postpartum. We modelled PrEP in scenario implementation analyses, with women testing HIV-negative being eligible for PrEP and incurring PrEP-related costs. Costs of infant prophylaxis were applied as a one-time cost to women diagnosed by delivery. Weekly ART costs were applied to HIV-infected infants throughout the 20-year time horizon. Both costs and DALYs were discounted at 3% annually [27] .

We converted infant infections and deaths into DALYs [28]. We estimated DALYs through 20 years of life using countryspecific WHO life tables and age-and HIV status-specific mortality estimates from published literature (Appendices S2 and S3) [29, 30] . We choose a time horizon of 20 years for comparability with other HIV cost-effectiveness analyses. We calculated the incremental cost-effectiveness ratio (ICER) as the change in costs divided by the change in health outcomes compared with the next best alternative. Strategies that were more costly and less effective ("strongly dominated") or less costly and less cost-effective ("weakly dominated") than an alternative strategy were considered inefficient [31] and eliminated from the calculations. We used country-specific cost-effectiveness thresholds based on the estimated opportunity cost of health investment foregone. We adopted thresholds of $500 (Kenya) [32, 33] ; $750 (South Africa), derived in [34] as During pregnancy 0.0001 [29] 0.0001 [29] 0.00002 [29] 0.00002 [29] Delivery through 6 weeks postpartum 0.0006 [68] 0.0002 [68] 0.0001 [68] 0.00003 [68] 6 weeks to 12 months postpartum 0.0001 [29] 0.0001 [29] 0.00002 [29] 0.00002 [29] Neonatal/infant mortality (per person-week) and survival probabilities Neonatal mortality, birth-6 weeks 0. 

We estimated the sensitivity of Scenario 2 results in Kenya and South Africa to uncertainty in key parameters by modelling 20% relative increases and decreases (bounded by 0 and 1 for probabilities). We also evaluated the sensitivity of model Scenarios 2 and 3 in each country to reductions in maternal HIV incidence and prevalence, and introduction of PrEP. We modelled PrEP use among 5%, 10% and 15% of all HIV-negative women. We additionally modelled a combination scenario of 15% PrEP use and 20% reduction in both maternal HIV prevalence and incidence. Last, to assess whether the benefits of retesting would change with an optimized HIV care cascade, we estimated the percentage of infections averted with retesting assuming 100% MCH attendance, test coverage, treatment initiation, retention, viral suppression and infant ARV prophylaxis coverage (Appendix S6).

This research involved no identifiable data, and as such does not constitute human subjects research.

Model results are presented in Table 3 and Figure 1 . In Kenya and South Africa, HIV retesting among women of HIVnegative or unknown status in late ANC, with catch-up testing through six weeks postpartum for women not tested in late ANC, is projected to prevent 19% and 12% of infant infections respectively. In both countries, ICERs associated with this retesting strategy ($166 and $289 per DALY averted for Kenya and South Africa respectively) fell below the cost-effectiveness thresholds. In these countries, adding one or two additional HIV retests postpartum provided only small incremental benefits (1 to 3 percentage point increase in HIV infections averted), and provision of one additional test at 14 weeks postpartum-dominated scenarios in which this test was offered at six, nine, or both six and nine months postpartum. HIV retesting in late ANC and every three months postpartum was the most effective and most costly strategy, exceeding the cost-effectiveness thresholds with ICERs of $7639 per DALY averted in Kenya and $11 985 in South Africa.

In Colombia, HIV retesting in late ANC with early postpartum catch-up averted a similar proportion of HIV infections (17%) as in Kenya and South Africa (19% and 12% respectively), whereas this strategy averted fewer infections in Ukraine (6%). However, due to the low HIV burden, the absolute number of infections prevented was small in both countries (23 in Colombia and 4 in Ukraine). Adding additional HIV retests followed a similar pattern to that of Kenya and South Africa, providing marginal incremental benefits but with substantially higher costs. All HIV retesting scenarios in Colombia and Ukraine were either dominated or exceeded the cost-effectiveness thresholds.

When restricting our results to women eligible for retesting (those without known HIV infection at the start of pregnancy), retesting in late ANC with early postpartum catch-up testing averted 23% of infections in Kenya, 19% in South Africa, 20% in Colombia and 24% in Ukraine. Consistent with our main results, additional postpartum retests increased the percent of maximum potential infections averted but with diminishing returns.

In uncertainty analyses, ICERs for HIV retesting in late ANC with catch-up retesting through six weeks postpartum ranged from $102 to $272/DALY averted in Kenya and from $156 to $799 for South Africa (Figure 2) . Changes in HIV test coverage, followed by infant ART cost, had the largest impact on ICERs. In all countries, increases in PrEP use, decreases in HIV incidence, and decreases in HIV prevalence resulted in larger ICERs (Figure 3a -h) and fewer HIV infections averted ( Figure S1 ). All scenario analyses of retesting in late ANC in Kenya and South Africa remained below the cost-effectiveness thresholds. Maternal retesting impact on MTCT was lowest when maternal incidence was reduced by 30%. Under an optimized care cascade, retesting in late ANC (with catch-up testing) and every three months postpartum averted 19% to 22% of infections in South Africa and Kenya (Appendix S6).

We evaluated the health and economic impact of maternal HIV retesting at varying frequencies and intervals in four countries representing a range of HIV prevalence. In the intermediate-and high-prevalence settings of Kenya and South Africa, our results indicate retesting in late ANC with catch-up testing through early postpartum should be prioritized to capture as many maternal seroconversions occurring during pregnancy as possible. In such settings, retesting later in the postpartum period can capture maternal infections acquired after delivery but should only be considered in addition to retesting earlier in pregnancy/postpartum. As retesting in late ANC with catch-up testing and quarterly postpartum (Scenario 7) most closely aligns with current South African guidelines [17], reducing the frequency of postpartum retesting may be effective in optimizing maternal HIV retesting with limited resources. In the low prevalence settings of Columbia and Ukraine, all HIV retesting strategies were dominated or greatly exceeded cost-effectiveness thresholds. Retesting in late ANC with catch-up testing prevented the largest number of infant HIV infections, but the absolute number was small (<25 infections annually).

Our model results also highlight limits on retesting as a strategy for EMTCT. While one-third of infant infections are attributable to incident maternal infections [4] , even with frequent testing throughout pregnancy and postpartum the proportion of infant infections averted due to retesting was only 27% in Kenya and 21% in South Africa after excluding MTCT attributed to women diagnosed with HIV prior to pregnancy. Optimizing MCH attendance and HIV care cascade parameters had little impact on these results. Antibody-based testing has low sensitivity during acute infection, when transmission risk is highest, making incident infections more difficult to detect. Our results thus suggest that achieving EMTCT will require additional interventions for prevention, such as PrEP, partner testing or diagnostics that facilitate earlier detection of incident infections. In Colombia and Ukraine, where postnatal risk of maternal HIV acquisition is low, as is subsequent MTCT risk due to low rates of breastfeeding, the impact of postpartum retesting is similar to antenatal retesting. Decisions on implementation of maternal retesting will depend on national priorities (such as EMTCT), programmatic goals and targets, and available resources, and countries with low HIV prevalence may consider retesting women at higher risk of HIV, including key populations (e.g. women who inject drugs, female sex workers) and those in serodiscordant relationships. Risk-based screening tools, such as the one developed for pregnant and postpartum Kenyan women [39] , may help identify such women during pregnancy or postpartum in both high and low prevalence settings.

Although our findings suggest maternal retesting is cost-effective in countries with high maternal HIV prevalence, there are several factors that need to be considered when implementing this strategy. First, country guidelines need to provide clear criteria on retesting eligibility and timing [10] . Countries should consider provider capacity to offer retesting given their workload and ensure monitoring and evaluation activities both document testing history and measure impacts of retesting. Providers who offer testing will need training to deliver post-test counselling messages that address HIV risk during pregnancy and breastfeeding and the need for future retesting, in addition to reinforcing HIV prevention messages. Emphasizing the importance of maternal retesting to prevent MTCT may also help providers prioritize retesting during busy ANC or postnatal care/infant immunization visits, including conveying elevated risks of HIV acquisition during pregnancy and breastfeeding and subsequent MTCT. Finally, test kit procurement and supply-chain management must be optimized to ensure test kit availability at facilities and that maternal retesting does not hinder other HIV testing efforts.

We found additional postpartum retesting incurs high costs per DALY averted, thus countries adopting this approach will need innovative and differentiated retesting models to optimize resource use [2] . Because HIV test coverage at first ANC visit is high in most countries, alternative delivery approaches to retesting may increase coverage among women who only attend one ANC visit, deliver at home, and/or do not return for MCH visits for postnatal care/infant immunizations. The WHO recommends self-testing as an additional testing approach [40]; however, to date few programmes have introduced self-tests for maternal retesting. While self-tests are unlikely to replace clinic-based rapid tests, they may play a complementary role in PMTCT programmes to increase access and acceptability of retesting and should be evaluated for maternal retesting in future studies. In the context of the COVID-19 response, due to declining and delayed facility visits in many settings, HIV self-tests are increasingly being considered as a way to provide maternal catch-up testing and retesting for programmes and should be considered and evaluated.

Our analysis has several strengths. We model delivery of HIV retesting at routine ANC and MCH visits, mitigating the costs incurred by retesting. A similar strategy could be adopted for HIV testing in conjunction with routine paediatric vaccination past the MCH visits included in our model. We assessed the impact of additional tests during the postnatal period, which is included in comparatively fewer national guidelines than retesting during late pregnancy/delivery [10] . Furthermore, we included countries with different HIV burden and geographic locations. While countries modelled are currently not implementing PrEP for pregnant and postpartum women at the national level, we assessed the impact of future PrEP scale-up on retesting strategies in sensitivity analyses. We also modelled reductions in maternal HIV incidence that could result from other HIV prevention interventions that may benefit pregnant/lactating women (i.e. condom use, risk reduction, partner HIV testing) [40] . Our conclusions were robust to varying assumptions about HIV prevalence, incidence and PrEP scale-up.

Our analysis also has several limitations. Some parameter values were assumed due to lack of empiric data; however, our estimates of MTCT rates were consistent with published estimates. While we modelled paediatric ART costs through 20 years, we modelled maternal ART costs only through HIV incidence refers to both incidence between onset of pregnancy and first antenatal care (ANC) visit, and incidence between first ANC and delivery, with both parameters varied by 20% for this analysis. HIV test coverage is the product of test acceptance and test kit being in-stock, with the composite parameter (test coverage) varied by 20% for this analysis. 12 months postpartum, as maternal outcomes were not the focus of our model and we assume MTCT ceases after 12 months. We did not model any changes in infant ARV and paediatric ART costs over this period, but our results were robust to changes in costs in sensitivity analyses. HIV diagnosis and linkage to care improves maternal health outcomes and may also prevent horizontal transmissions, neither of which are not captured by our model. While our intent was to model retesting in late pregnancy, tests in late ANC capture both incident maternal infections and established infections not detected at first ANC due to late presentation (after the gestational age of first ANC modelled) or absence of testing at first ANC. Furthermore, our model only allows for testing at two antenatal time points, with parameterization corresponding to re-testing in the third trimester in South Africa and Kenya, and in the second trimester in Colombia and Ukraine. As our model did not stratify by demographic or HIV risk factors, our results may not represent the impact of maternal retesting in concentrated epidemics within a country, nor of retesting prioritized for key populations. Our choice of countries was constrained by data availability and may not be generalizable to other settings where key parameters differ substantially from those modelled. We did not explicitly model time since maternal ART initiation and therefore may not have captured more complex temporal patterns in maternal ART use. Our model assumed all HIV-infected infants were equally likely to receive ART regardless of whether their mother's HIV-positive status was known or unknown. As early diagnosis and treatment will avert DALYs even if transmission is not prevented, our results are conservative in this regard. Our results are presented as point estimates without accompanying uncertainty. As several of our model parameters were based on expert elicitation or assumptions, and we expect many are correlated, we felt valid parameterization of a formal probabilistic sensitivity analysis was out of reach. Finally, the cost-effectiveness thresholds used in this analysis are approximate and may not accurately reflect country-specific willingness-to-pay under budget constraints. Affordability constraints may prevent some countries from implementing interventions below these thresholds. Alternatively, in efforts to achieve EMTCT, programmes may consider incurring high costs to implement retesting strategies above the cost-effectiveness thresholds used in this analysis.

We found retesting women in late ANC followed by catch-up testing though six weeks postpartum is a cost-effective approach to PMTCT in Kenya and South Africa and should be implemented by PMTCT programmes. Universal maternal retesting was not cost-effective in Colombia and Ukraine; these counties may consider retesting if resources are available as retesting does contribute to EMTCT efforts, which would align with their current national retesting guidance. Additional empiric data on implementing maternal retesting guidelines is needed to refine model inputs, to evaluate retesting strategies for key populations at high HIV risk, and to revise retesting guidelines as incidence declines in high-burden countries. 

Elimination of mother-to-child transmission. UNICEF

Global AIDS update 2019 -Communities at the centre

The contribution of maternal HIV seroconversion during late pregnancy and breastfeeding to mother-to-child transmission of HIV

UNAIDS. Start free stay free AIDS free 2019 report. Joint United Nations Programme on HIV/AIDS (UNAIDS)

Incident HIV during pregnancy and postpartum and risk of mother-to-child HIV transmission: a systematic review and meta-analysis

Joint United Nations Programme on HIV/AIDS (UNAIDS)

Should HIV testing for all pregnant women continue? Cost-effectiveness of universal antenatal testing compared to focused approaches across high to very low HIV prevalence settings

Antiretroviral drugs for treating pregnant women and preventing HIV infection in infants: towards universal access towards universal access

Consolidated guidelines on HIV testing services

Retest and treat: a review of national HIV retesting guidelines to inform elimination of mother-to-child HIV transmission (EMTCT) efforts

Cost-effectiveness of a repeat HIV test in pregnancy in India

The cost-effectiveness of repeat HIV testing during pregnancy in a resource-limited setting

Cost-effectiveness of HIV rescreening during late pregnancy to prevent motherto-child HIV transmission in South Africa and other resource-limited settings

Sexual behavior and vaginal practices during pregnancy and postpartum: implications for HIV prevention strategies

Increased risk of HIV acquisition among women throughout pregnancy and during the postpartum period: a prospective per-coital-act analysis among women with HIV-infected partners

National Department of Health. National HIV Testing Services: Policy. Republic of South Africa: Department of Health

Minstry of Health, National AIDS & STI Control Programme. Guidelines on Use of Antiretroviral Drugs for Treating and Preventing HIV infection in Kenya

Report on pre-validation of elimination of mother-to-child transmission of HIV in Ukraine

Gu ıa de pr actica cl ınica (GPC) basada en la evidencia cient ıfica para la atenci on de la infecci on por VIH/Sida en adolescentes (con 13 años o m as de edad) y adultos. Gu ıa para profesionales de la salud GPC-2014-39. Libert y Orden

Consolidated guidelines on the use of antiretroviral drugs for treating and preventing HIV infection

Retention in care during the first 3 years of antiretroviral therapy for women in Malawi's option B+ programme: an observational cohort study

Nondisclosure to male partners and incomplete PMTCT regimens associated with higher risk of mother-to-child HIV transmission: a national survey in Kenya

Estimating the impact of antiretroviral treatment on adult mortality trends in South Africa: a mathematical modelling study

Elimination of mother-to-child transmission of HIV and syphilis in the Americas. Update

Bureau of Labor Statistics. CPI Inflation Calculator

Department of Information Evidence and Research. WHO methods and data sources for global burden of disease estimates

World Health Organization. Country Life Tables

Mortality of infected and uninfected infants born to HIV-infected mothers in Africa: a pooled analysis

Methods for the economic evaluation of health care programmes

Cost-effectiveness of public-health policy options in the presence of pretreatment NNRTI drug resistance in sub-Saharan Africa: a modelling study

Country-level cost-effectiveness thresholds: initial estimates and the need for further research

Revealed willingness-to-pay versus standard cost-effectiveness thresholds: evidence from the South African HIV investment case

Estimation of supply side cost effectiveness threshold in Ukraine: perspective use in health care decision-making

Consolidated health economic evaluation reporting standards (CHEERS)-explanation and elaboration: a report of the ISPOR health economic evaluation publication guidelines good reporting practices task force

A risk assessment tool for identifying pregnant and postpartum women who may benefit from preexposure prophylaxis

The 2017 National antenatal sentinel HIV survey

Prevalence of HIV (Ages 15-49) -South America

HIV acquisition during pregnancy and postpartum is associated with genital infections and partnership characteristics

Evolution of antiretroviral therapy services for HIV-infected pregnant women in Cape Town, South Africa

Mother-to-child transmission of HIV in Kenya: results from a nationally representative study

Mother-to-child transmission of HIV-1 infection during exclusive breastfeeding in the first 6 months of life: an intervention cohort study

Antenatal HIV-1 RNA load and timing of mother to child transmission; a nested case-control study in a resource poor setting

A case series of 104 women infected with HIV-1 via blood transfusion postnatally: high rate of HIV-1 transmission to infants through breast-feeding

utero and intra-partum HIV-1 transmission and acute HIV-1 infection

during pregnancy: using the BED capture enzyme-immunoassay as a surrogate marker for acute infection

Timing of mother-to-child transmission of HIV-1 and infant mortality in the first 6 months of life in Harare

Kenya Ministry of Health, Kenya National AIDS Control Council, Kenya Medical Research Institute, and Kenya National Council for Population and Development. Kenya Demographic and Health Survey

Ukraine Demographic and Health Survey

ETMI. PLUS. Estrategia Nacional para la Eliminaci on de la Transmisi on Materno Infantil del VIH, la s ıfilis cong enita, la hepatitis B y la enfermedad de Chagas. Comportamiento de la Transmisi on Materno Infantil del VIH en Colombia. Medici on de la Cohorte

Factors associated with time to achieve an undetectable HIV RNA viral load after start of antiretroviral treatment in HIV-1-infected pregnant women

Impact of HIV-status disclosure on HIV viral load in pregnant and postpartum women on antiretroviral therapy

Using mobile phones and short message service to deliver self-management interventions for chronic conditions: a metareview

Infant feeding by South African mothers living with HIV: implications for future training of health care workers and the need for consistent counseling

Antiretroviral therapy uptake and coverage in four HIV community cohort studies in sub-Saharan Africa

Institute for Health Metrics and Evaluation. Global burden of disease estimates

Evaluating the effect of HIV prevention strategies on uptake of HIV counselling and testing among male most-at-risk-populations in Nigeria; a cross-sectional analysis

Incremental treatmet costs for HIV-infected women initiating antiretroviral therapy during pregnancy: A 24-month micro-costing cohort study for a maternal and child health clinic in Kenya

The per-patient costs of HIV services in South Africa: Systematic review and application in the South African HIV Investment Case

The role of costing in the introduction and scale-up of HIV pre-exposure prophylaxis: evidence from integrating PrEP into routine maternal and child health and family planning clinics in western Kenya

Effectiveness and cost effectiveness of oral pre-exposure prophylaxis in a portfolio of prevention programs for injection drug users in mixed HIV epidemics

Additional information may be found under the Supporting Information tab for this article. Figure S1. Scenario implementation analysis of maternal HIV retesting on number of infant HIV infections averted. (a) Decreasing HIV prevalence in Kenya and South Africa, (b) Decreasing HIV prevalence in Colombia and Ukraine, (c) Decreasing HIV incidence in Kenya and South Africa, (d) Decreasing HIV incidence in Colombia and Ukraine, (e) Increasing PrEP use in Kenya and South Africa, (f) Increasing PrEP use in Colombia and Ukraine, (g) Decreasing HIV prevalence and incidence, increasing PrEP use in Kenya and South Africa, (h) Decreasing HIV prevalence and incidence, increasing PrEP use in Colombia and Ukraine

pre-exposure prophylaxis; Maternal HIV retesting Scenarios 2 (retesting in late ANC/delivery/six weeks postpartum) and 3 (retesting in late ANC/delivery/six weeks postpartum and at 6 months postpartum) modelled