key: cord-0723485-76x3pgo6 authors: Borowicz-Reutt, Kinga K. title: How Antidepressant Drugs Affect the Antielectroshock Action of Antiseizure Drugs in Mice: A Critical Review date: 2021-03-03 journal: Int J Mol Sci DOI: 10.3390/ijms22052521 sha: 503b072592fae44d1344330c41ebd634240a9d15 doc_id: 723485 cord_uid: 76x3pgo6 Depression coexists with epilepsy, worsening its course. Treatment of the two diseases enables the possibility of interactions between antidepressant and antiepileptic drugs. The aim of this review was to analyze such interactions in one animal seizure model—the maximal electroshock (MES) in mice. Although numerous antidepressants showed an anticonvulsant action, mianserin exhibited a proconvulsant effect against electroconvulsions. In most cases, antidepressants potentiated or remained ineffective in relation to the antielectroshock action of classical antiepileptic drugs. However, mianserin and trazodone reduced the action of valproate, phenytoin, and carbamazepine against the MES test. Antiseizure drug effects were potentiated by all groups of antidepressants independently of their mechanisms of action. Therefore, other factors, including brain-derived neurotrophic factor (BDNF) and glial-derived neurotrophic factor (GDNF) modulation, should be considered as the background for the effect of drug combinations. It is estimated that depression affects 350 million people worldwide. According to the World Health Organization data, it will be the most common disease in the world by 2030. Currently, depression takes a million lives a year from suicide. Depressive disorders are diagnosed in 2% of children, 8% of teenagers, and 10% of pregnant women. It is worth emphasizing that the percentage of pharmacological antidepressant treatment is also continuously increasing in these groups of patients [1] . On the other hand, depression and related disorders are the most common comorbidities in epilepsy patients. The coexistence of these disease entities reaches 30% and depressive episodes have begun to be considered a marker of drug-resistant epilepsy [2] . Evidently, depression in epilepsy patients requires appropriate treatment, including pharmacotherapy. Antidepressant drugs are used not only in the treatment of endogenous depression and depression accompanying neurodegenerative diseases, but also in that of phobias, compulsive-obsessive disorders, insomnia (a frequent mask of depression), fibromyalgia, and even obesity. The use of anticonvulsants also goes well beyond epilepsy and includes seizures accompanying other disturbances of the central nervous system (tumors, ischemia, and trauma), neuralgias, neuropathies, or eclampsia [2, 3] . Such a wide use markedly increases the likelihood of interactions occurring between antiepileptic and antidepressant drugs. Neurons in the brain of epilepsy patients are characterized by an increased excitability that may constitute a genetic background of this disorder. Therefore, every factor that increases the neuronal excitability may contribute to increases in the seizure frequency and severity. Such factors are, for example, a markedly increased body temperature (febrile seizures and heat shock), hypoglycemia, or drugs affecting the neuronal membrane potential, including antidepressant drugs. Currently, it is widely recognized that most antidepressant drugs are entirely safe in epilepsy patients at therapeutic doses. The confirmed exceptions are bupropion, clomipramine, amoxapine, and maprotiline [4, 5] . A great The review is mainly based on the author's own research. Medline and Science Direct databases were searched for additional articles using the term "antidepressant drugs and maximal electroshock". Out of 71 (Medline) and 157 (Science Direct) results, 24 articles were selected as the most relevant. Tricyclic drugs of the old generation (e.g., amitriptyline, imipramine, and desipramine) block the reuptake of serotonin and norepinephrine from the synaptic cleft. Maprotiline, which is a tetracyclic antidepressant, is an inhibitor of norepinephrine reuptake. However, adverse effects mainly related to their anticholinergic properties limited the use of the two types of drug in psychiatric practice. In experimental conditions, amitriptyline (20-30 mg/kg, i.p.) and imipramine (30-40 mg/kg) exhibited their own anticonvulsant effects, significantly increasing the threshold for electroconvulsions. Desipramine (up to 40 mg/kg) remained ineffective in this aspect. The three tricyclic antidepressants, at doses subeffective in the electroconvulsive test (10 and 20 mg/kg, respectively), distinctly potentiated the protective efficacy of valproate against the MES. Pharmacokinetic data are incomplete. It is only known that desipramine (20 mg/kg) did not affect the brain concentration of valproate [7] . Furthermore, in another study, imipramine (17.5-25 mg/kg) showed an anticonvulsant action against the MES in mice. However, higher doses induced a neurotoxic effect, including clonic seizures [8] . The antielectroshock effect of combinations of amitriptyline and phenobarbital or carbamazepine was reported by Tregubov and Kolla [9] . Doxepin was effective against maximal electroshock-induced seizures, with an estimated 50% effective dose (ED 50 ) value of 6.6 mg/kg [10] . Acute and chronic injections of fluoxetine (10 mg/kg) did not modify the threshold for electroconvulsions in mice [11] . On the other hand, this antidepressant, when applied once with the dose range of 15-25 mg/kg, significantly increased the threshold [12] . In contrast, chronic (14-day) treatment with fluoxetine (up to 20 mg/kg) did not affect this parameter [13] . A recent publication revealed that raloxifene, which is a selective modulator of estrogen receptors and a potential antiviral medication in the treatment of coronavirus disease 2019 (COVID- 19) , enhanced the action of fluoxetine (14 mg/kg) in the MES test [14] . Fluoxetine, when applied once at subeffective doses against the electroconvulsive threshold, significantly enhanced the anticonvulsive activity of carbamazepine, phenobarbital, and phenytoin, but not valproate, against the MES in mice [12, 15] . The chronic administration of this antidepressant (15-20 mg/kg) led to an enhancement of the protective activity of all four classic antiepileptic drugs [13] . In another study, fluoxetine, given either acutely or chronically at the lower dose of 10 mg/kg, did not affect the action of phenytoin in the MES test [11] . A pharmacokinetic verification of interactions indicated that acute administration of the antidepressant significantly increased brain levels of carbamazepine and phenobarbital, while chronic treatment elevated brain concentrations of all four antiepileptic drugs [12, 13] . For other selective serotonin reuptake inhibitors, the only data are that paroxetine did not affect the MES-induced convulsions in mice [16] . Both acute and chronic venlafaxine (12.5-25 mg/kg) raised the electroconvulsive threshold in mice. At subprotective doses, this antidepressant enhanced the action of valproate against MES-induced seizures in mice. At the lowest effective doses, venlafaxine additionally intensified the action of carbamazepine and phenobarbital. However, the interaction with phenobarbital could be partially due to pharmacokinetic events, since venlafaxine increased the brain concentration of phenobarbital. In the same study, chronic (14-day) treatment with venlafaxine enhanced the action of phenytoin, despite decreasing the brain level of this antiepileptic drug [17] . Milnacipran (10 mg/kg), when given once, but not repeatedly, raised the electroconvulsive threshold in mice. Furthermore, acute but not chronic treatment with this antidepressant potentiated the action of four first-generation antiepileptic drugs against the MES test in mice. The anti-electroshock effects of carbamazepine and phenobarbital were enhanced by subthreshold doses of milnacipran. Higher doses of the antidepressant were required to affect the action of valproate and phenytoin. Interestingly, no changes in brain concentrations of antiepileptic drugs were observed, so the contribution of pharmacokinetic events to revealed interactions is rather unlikely [18] . Duloxetine exhibited an anticonvulsant action, with the estimated ED 50 value of 48.21 mg/kg in the MES test in mice. The single administration of duloxetine (6.25-25 mg/kg) significantly increased the anti-electroshock effects of valproate, carbamazepine, and oxcarbazepine. Pretreatment with an inhibitor of gamma-aminobutyric acid (GABA) synthesis significantly increased the ED 50 of duloxetine, suggesting the role of the GABAergic system in the mechanism of duloxetine's action [19] . An increase in the electroconvulsive threshold was observed when reboxetine (8-16 mg/kg) was applied only once, not repeatedly. In a continuation of this study, the influence of this antidepressant on the antielectroshock action of chosen antiepileptic drugs was evaluated. A single injection of reboxetine at subthreshold doses enhanced the effectiveness of carbamazepine, valproate, and phenobarbital. At higher doses, the antidepressant drug potentiated the action of phenytoin. The chronic (14-day) administration of reboxetine only enhanced the action of carbamazepine [20] . The acute administration of bupropion led to a biphasic effect in the MES test in mice. On the one hand, this antidepressant demonstrated clear antielectroshock activity, with an ED 50 value of 19.4 mg/kg. At higher doses, bupropion was proconvulsant, with a 97% convulsive dose (CD 97 ) value of 139.5 mg/kg [21, 22] . When the antidepressant was administered chronically (5 mg/kg), it increased the threshold for electroconvulsions and enhanced the antielectroshock action of felbamate, lamotrigine, and topiramate. The interaction with lamotrigine could be at least partially of a pharmacokinetic nature. However, intensification of the effect of topiramate occurred, despite decreased brain concentrations of this antiepileptic [23] . Given in a single injection, moclobemide (62.5 and 75 mg/kg) increased the electroconvulsive threshold. In contrast, chronic treatment with this antidepressant did not influence the threshold. Acute moclobemide applied at subthreshold doses (up to 50 mg/kg) enhanced the antielectroshock effects of carbamazepine, valproate, and phenobarbital. Chronic moclobemide (37.5-75 mg/kg) increased the action of all four antiepileptic drugs. All revealed interactions, except for those between moclobemide and phenobarbital, seem to have a pharmacokinetic nature, because the antidepressant drug, either in acute or chronic treatment, increased the brain concentrations of respective antiepileptic drugs [24] . Mianserin increases serotonergic and noradrenergic neurotransmission by acting as an antagonist at the serotonin 5-HT 2 and adrenergic α 2 presynaptic and somatodendritic auto-and heteroreceptors. Acute mianserin (30-40 mg/kg) increased the electroconvulsive threshold in mice, while chronic treatment led to the opposite effect. Moreover, a single application of this antidepressant at subthreshold doses enhanced the antielectroshock action of carbamazepine, valproate, and phenytoin, but not that of phenobarbital. In contrast, chronic (14-day) treatment reduced the effect of valproate and phenytoin against maximal electroshock. All revealed interactions seemed to have a pharmacodynamic nature [25] . Trazodone is an antidepressant inhibiting serotonin transport and blocking 5HT 2 receptors. Chronic (but not acute) treatment with trazodone (10-40 mg/kg) increased the threshold for electroconvulsions, exhibiting a clear antiseizure action. However, when applied acutely or chronically (at its subthreshold doses), this antidepressant diminished the antielectroshock activity of carbamazepine and phenytoin. The effects of valproate and phenobarbital remained unchanged. Pharmacokinetic interactions between trazodone and the antiepileptic drugs were not observed [26] . Tianeptine is an antidepressant drug that contradicts the monoaminergic theory of depression. This medication even enhances serotonin uptake from the synaptic cleft. Tianeptine seems to act as an agonist of opioid receptors and mediate a variety of neurobiological effects, including the restoration of plasticity in the amygdala, decrease in stress-induced glutamate release, and reversal of stress-induced dystrophy of hippocampal CA3 dendrites [27] . The antiseizure effect of tianeptine can also be explained by purinergic (through adenosine A1 receptors), glutamatergic, and nitrergic mechanisms [28] . Neither acute nor repeated treatment with tianeptine (up to 50 mg/kg) affected the electroconvulsive threshold in mice. Nevertheless, both acute and chronic application potentiated the antielectroshock action of carbamazepine, valproate, and phenobarbital, but not phenytoin. The action of phenobarbital was even enhanced when lowering its brain level. Brain concentrations of remaining antiepileptics were not affected by tianeptine [28] . All data can be found in Tables 1 and 2 . There are currently no data on the effects of drugs such as esketamine, allopregnanolone (brexanolone), vortioxetine, or agomelatine in the MES test. Interactions between the above-mentioned drugs and antiepileptic drugs have also not been studied in this seizure model. However, allopregnanolone interacted synergistically with tiagabine in two other models of electricaly-induced convulsions, including hippocampus kindling and 6-Hz seizure models in mice [29] . Table 1 . Effects of antidepressant drugs on the electroconvulsive threshold and the antielectroshock action of antiepileptic drugs. Acute treatment means that a given antidepressant drug was injected only once, while chronic treatment means that this drug was applied for 14 days. Brain concentrations of antiepileptic drugs were only measured in the case of positive antielectroshock interactions. AEDs, antiepileptic drugs; CBZ, carbamazepine; ECT, the electroconvulsive threshold; FBM, felbamate; LTG, lamotrigine; nt, not tested; PB, phenobarbital; PHT, phenytoin; TPM, topiramate; VPA, valproate; ↑, increased electroconvulsive threshold/potentiated antielectroshock action/increased brain concentration; ↓, decreased electroconvulsive threshold/reduced antielectroshock action/decreased brain concentration; ↔, no effect on the aforementioned parameters. However, ketamine, which is the parent drug for esketamine mostly used as an injectable anesthetic, was investigated in the MES test. Ketamine exhibited the characteristic of an antiepileptic drug in this test and synergistically interacted with valproate and carbamazepine. Combinations of ketamine and phenytoin or phenytoin led to additivity. The determined interactions do not appear to be dependent on pharmacokinetic events, since none of the injectable anesthetics changed the free plasma levels of respective antiepileptic drugs [30] . The most obvious mechanism used to be associated with enhanced serotonergic neurotransmission. Several studies proved that animals with genetically conditioned seizures exhibited serotonin and/or noradrenergic deficits, while enhanced serotonergic and/or noradrenergic transmission attenuated seizures [31] . However, the issue of serotonergic's action seems to be more complicated. For instance, tool substances HBK-14 and HBK-15, which are two triple 5-HT 1A , 5-HT 7 , and 5-HT 3 antagonists (HBK-14 and HBK-15), were reported to have potent antidepressant-like and anticonvulsant properties in a variety of animal models [32] . On the other hand, mirtazapine activating serotonergic 5-HT 1A receptors and blocking 5-HT 2 and 5-HT 3 receptors did not affect seizures in the maximal electroshock [33] . The results of the two studies considered together may suggest that the anticonvulsant action could be mainly related to blocking of the 5-HT 7 receptor. The probable anticonvulsant effect of dopamine is believed to be realized through activation of the D 2 receptor, while that of noradrenaline is believed to occur through the stimulation of alpha 2 -and beta 2 -adrenoreceptors. Acetylcholine may play a dual role in the pathogenesis of experimental seizures. Pilocarpine, which is a muscarinic agonist, induces primary limbic seizures with secondary generalization. However, another muscarinic agonist, called carbachol, reduced epileptiform discharges in the model of absence seizures [31] . On the other hand, mechanisms conditioning the anticonvulsant action, such as the blocking of sodium or calcium channels and activation of potassium channels, may contribute to antidepressant-like behavior in experimental animals. Moreover, ketamine, which is an antagonist of glutamatergic N-methyl-D-aspartate (NMDA) receptors, exhibits not only anticonvulsant, but also antidepressant-like, effects in animal models [31] . The antielectroshock action of ketamine may also be associated with its action on D 2 /sigma, GABA A , and GABA B [34] . There are some reports confirming the influence of antidepressant drugs on glutamate release in different brain regions. For instance, fluoxetine decreased glutamate synaptic release. This effect can be explained by changes in the structure of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, the effector of vesicle docking, and fusion at presynaptic membranes [35] . The same mechanism seems to be involved in the action of chronically administered venlafaxine. This drug was reported to dampen glutamate release from rat hippocampal synaptic terminals and the frontal/prefrontal cortex [36] . Chronic (but not acute) treatment with fluoxetine, reboxetine, and desipramine reduced the depolarization-evoked release of glutamate in the rat hippocampus [37] . In turn, milnacipran reduced the amplitude of NMDA-, but not α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-mediated currents in lamina II neurons of rat spinal cord slices [38] and blocked activated NMDA receptors in Xenopus oocytes [39] . Bupropion inhibited the 4-aminopyridine-evoked release of glutamate from rat cerebrocortical nerve terminals [40, 41] . Moreover, bupropion increased the seizure latency and decreased the seizure severity in the kainic acid rat seizure model, representing an animal model for temporal lobe epilepsy [38] . Trazodone, as a partial agonist of presynaptic 5-HT 2A heteroreceptors, decreased the release of glutamate from rat cerebellar mossy fibers [42] . Finally, tianeptine modulates glutamatergic transmission through the activation of mu opioid receptors [43] . On the other hand, the activity of antidepressants with a serotonergic mechanism of action (escitalopram and milnacipran) can be inhibited by NMDA agonists, while antidepressants with a noradrenergic mechanism of action (imipramine and reboxetine) can be inhibited by AMPA antagonists [44] . This may be indirect proof that antidepressant drugs diminish glutamatergic transmission. Similarly, seizure inhibition and the antidepressant-like action may be mediated by the enhancement of GABA-ergic neurotransmission. In contrast, GABA deficits were reported to inhibit serotonergic and noradrenergic neurotransmission in the brain. More-over, the dual anticonvulsant/antidepressant effect can be attributed to the action of some neurotrophins (brain-derived neurotrophic factor (BDNF) and glial-derived neurotrophic factor (GDNF)) and neurosteroids (allopregnanolone) [31] . A plethora of data indicate that neurotrophic factors, above all the brain-derived neurotrophic factor (BDNF) and glial-derived neurotrophic factor (GDNF), participate in processes of brain plasticity and epileptogenesis. Interestingly, BDNF shows excitatory effects in in vitro conditions, neuronal cultures, and animal brain slices. Furthermore, the acute intracerebral administration of BDNF induces seizures in mice. In contrast, the chronic infusion of BDNF reduces the neuronal excitability, which can be related to the downregulation of tropomyosin receptor kinase B (TrkB, the receptor for BDNF) and, in consequence, increased brain concentrations of neuropeptide Y (NPY) and altered chloride conductance. Furthermore, NPY proved its antiseizure and antiepileptogenic effects in a variety of animal models. Therefore, higher serum BDNF in epilepsy patients, correlated with the severity of disease, may reflect adaptations to seizures. Moreover, inhibiting BDNF-TrkB signaling and simultaneous activation of the NPY system could become a new treatment pathway for epilepsy [45, 46] . In fact, some widely recognized antiepileptic drugs may act this way. For instance, valproate was reported to increase the BDNF level and then downregulate BDNF/TrkB protein signaling in the hippocampus [47] . Interesting results were shown in the study of Tegkul et al. [48] , where 14-day treatment with subconvulsive doses of pentetrazole decreased the brain level of BDNF. In contrast, BDNF levels were increased when pentetrazole was co-administered with midazolam or levetiracetam [49] . According to experimental data, an intrahippocampal injection of GDNF, as well as the implementation of viral vectors expressing GDNF, can suppress chemically-and electrically-induced seizures [49] . A wide range of research has indicated that chronic treatment with different classes of antidepressant drugs increases brain concentrations of BDNF. In one of the first reports, tranylcypromine, sertraline, desipramine, and mianserin significantly increased BDNF mRNA in the rat hippocampus [50] . Moclobemide increased the BDNF level in in vitro experiments conducted on hippocampal progenitor cells [51] . Fluoxetine increased the hypothalamic BDNF expression in mice [52] , while milnacipran enhanced BDNF protein and mRNA levels in the mouse cerebral cortex [53] . On the other hand, duloxetine activated the BDNF protein expression in brains of rats subjected to the methamphetamine model of anxiety [54] . Desipramine, fluoxetine, and tianeptine increased the BDNF mRNA and protein expression in stressed rats [55] . Finally, in clinical conditions, increased serum BDNF levels were detected in 30 patients treated with bupropion [56] . Not only BDNF, but also GDNF, are being considered as potential therapeutic agents in the antiepileptic strategy. Similarly to BDNF, the brain levels of GDNF are elevated by a variety of antidepressants with different mechanisms of action, including amitriptyline, clomipramine, mianserin, fluoxetine, paroxetine [57] [58] [59] , and mirtazapine [60] . Interestingly, escitalopram increased the serum levels of both GDNF and BDNF in obsessive-compulsive disorder in rats [61] . Fluoxetine, imipramine, and milnacipran upregulated BDNF and GDNF in the striatum and substantia nigra of rats in the MPTP model of Parkinson's disease [62] . Probable mechanisms of the antiseizure action of antidepressant drugs and their influence on the antielectroshock action of antiepileptics are shown in Figure 1 . Interestingly, escitalopram increased the serum levels of both GDNF and BDNF in obsessive-compulsive disorder in rats [61] . Fluoxetine, imipramine, and milnacipran upregulated BDNF and GDNF in the striatum and substantia nigra of rats in the MPTP model of Parkinson's disease [62] . Probable mechanisms of the antiseizure action of antidepressant drugs and their influence on the antielectroshock action of antiepileptics are shown in Figure 1 . Doses of antidepressant drugs affecting the action of antiepileptics have been converted to human doses according to Raegan-Shaw et al. [63] . Only in the case of reboxetine and tianeptine did the doses used in experiments exceed the maximal single doses of these antidepressants. Therefore, it seems that the likelihood of interactions is relatively high in clinical conditions (Table 3) . Doses of antidepressant drugs affecting the action of antiepileptics have been converted to human doses according to Raegan-Shaw et al. [63] . Only in the case of reboxetine and tianeptine did the doses used in experiments exceed the maximal single doses of these antidepressants. Therefore, it seems that the likelihood of interactions is relatively high in clinical conditions (Table 3) . In general, most antidepressant drugs, given either acutely or chronically, exhibited their own antiseizure action, increasing the electroconvulsive threshold in mice. The exception was chronic mianserin, which led to a proconvulsive effect in this test. From a theoretical point of view, there is one possible explanation for this-mianserin behaves as an antagonist of the dopamine D 2 receptor (DrugBank https://go.drugbank.com/drugs/DB06148, accessed on 21 January 2021). The anticonvulsant action of antiepileptic drugs was enhanced by both acute and chronic treatment with antidepressant drugs. Therefore, adaptive changes with monoaminergic receptors necessary to develop an antidepressant effect do not seem to be crucial for their antielectroshock activity. With regards to conventional antiepileptic drugs, their antiseizure action was potentiated by almost all groups of antidepressants. However, fluoxetine, which is a representative of selective serotonin reuptake inhibitors, increased the brain concentration of all antiepileptics tested, including valproate, carbamazepine, phenytoin, and phenobarbital. Therefore, interactions between fluoxetine and classical antiseizure drugs have a strong pharmacokinetic component. Less pharmacokinetic interactions were found when antiepileptic drugs were combined with representatives of remaining groups of antidepressants (venlafaxine, milnacipran, duloxetine, reboxetine, mianserin, and even moclobemide). The common mechanism of action of these drugs is the enhancement of noradrenergic transmission. Therefore, the question of whether it is not noradrenaline that is responsible for the enhancement of the antiseizure action arises. Nevertheless, the fact that chronic mianserin decreased the action of valproate and phenytoin does not support this hypothesis. It can only be speculated that this effect must be related to the downregulation of 5-HT 2 and α 2 receptors. It is even more difficult to explain why trazodone, increasing the electroconvulsive threshold after chronic treatment, attenuated the antielectroshock action of carbamazepine and phenytoin. However, recent data showed that trazodone enhanced seizures in a genetic WAG/Rij ((Wistar Albino Glaxo from Rijswijk) rat model of absence epilepsy [64] . Perhaps the mechanisms responsible for this proconvulsive action are also involved in interactions with carbamazepine and phenytoin. It remains unclear why just phenytoin is so prone to the attenuating action of mianserin and trazodone. Simultaneously, phenytoin also seemed to be the most susceptible to the potentiating action of remaining antidepressant drugs. Further studies are required to elucidate this issue. Practically, antidepressant drugs enhanced, to varying degrees, the action of all antiepiletics tested, regardless of their main mechanism of action-sodium channel blockade, enhancement of GABA-ergic, or attenuation of glutamatergic neurotransmission. However, the strongest interactions were observed between acute/chronic tianeptine and phenobarbital, venlafaxine, and phenytoin, as well as between chronic bupropion and topiramate. In these cases, the antielectroshock action of phenobarbital and topiramate was enhanced, despite their lowered brain concentrations. Interestingly, phenobarbital and topiramate share one mechanism of action-the two drugs bind to the GABA receptor subunit alpha-1. Perhaps the influence on this subunit contributes to such a strong response to antidepressant drugs. It is quite surprising, however, that tianeptine, which is an antidepressant increasing the serotonin uptake, potentiates the action of antiepileptics as well as or even better than antidepressant drugs enhancing monoaminergic transmission. This may suggest that factors other than monoaminergic mechanisms are involved in these interactions. The most obvious assumption is that antiepileptic and antidepressant drugs synergistically decrease glutamatergic and/or increase GABA-ergic neurotransmission. The two groups of drugs may also increase the brain BDNF and/or GDNF expression. Both hypotheses require verification in further research. Most antidepressant drugs tested exhibited an antiseizure or neutral action in the electroconvulsive threshold test in mice. The exception was chronic mianserin, which displayed a significant proconvulsant effect. Either acute or chronic treatment with antidepressants potentiated or did not affect the antielectroshock action of classical anti-epileptic drugs. Only chronic mianserin and acute and chronic trazodone diminished the action of valproate, phenytoin, and carbamazepine in the MES test. There are no premises that the main mechanisms involved in the antidepressant and/or antiseizure action may be responsible for interactions between the two groups of medications. Therefore, it can be assumed that other factors provide the background for the effect of drug combinations. BDNF and GDNF modulate both antiseizure and antidepressant-like effects in experimental conditions. Their role in these interactions, as very likely, should be confirmed in further investigations. Management of treatment-resistant depression in children and adolescents Association of depression and treated depression with epilepsy and seizure outcomes: A multicohort analysis Epilepsy coexisting with depression Seizure incidence in psychopharmacological clinical trials: An analysis of Food and Drug Administration (FDA) summary basis of approval reports Most antidepressant drugs are safe for patients with epilepsy at therapeutic doses: A review of the evidence Effect of monoamine reuptake inhibition and α 1 blockade on respiratory arrest and death following electroshock-induced seizures in mice Influence of antidepressant drugs on seizure susceptibility and the anticonvulsant activity of valproate in mice Biphasic effects of imipramine in experimental models of epilepsy The effect of antidepressants on the experimental anticonvulsant activity and acute toxicity of anticonvulsants Characterization of the anticonvulsant activity of doxepin in various experimental seizure models in mice Effect of fluoxetine on maximal electroshock seizures in mice: Acute vs chronic administration Fluoxetine enhances the anticonvulsant effects of conventional antiepileptic drugs in maximal electroshock seizures in mice Chronically administered fluoxetine enhances the anticonvulsant activity of conventional antiepileptic drugs in the mouse maximal electroshock model Raloxifene potentiates the effect of fluoxetine against maximal electroshock induced seizures in mice Fluoxetine, a selective serotonin-uptake inhibitor, enhances the anticonvulsant effects of phenytoin, carbamazepine, and ameltolide (LY201116) Behavioral pharmacological properties of the novel antidepressant paroxetine, a selective 5-HT uptake inhibitor Effect of acutely and chronically administered venlafaxine on the anticonvulsant action of classical antiepileptic drugs in the mouse maximal electroshock model Effect of acute and chronic treatment with milnacipran potentiates the anticonvulsant activity of conventional antiepileptic drugs in the maximal electroshock-induced seizures in mice Anticonvulsive activity of duloxetine: A new choice for the epileptic patients with depression Reboxetine and its influence on the action of classical drugs in the mouse maximal electroshock model Convulsant and anticonvulsant effects of bupropion in mice Bupropion-induced convulsions: Preclinical evaluation of antiepileptic drugs Low dose of bupropion significantly enhances the anticonvulsant activity of felbamate, lamotrigine and topiramate in mice Acute and chronic treatment with moclobemide, a reversible MAO-inhibitor, potentiates the antielectroshock activity of conventional antiepileptic drugs in mice Acute and chronic treatment with mianserin differentially affects the anticonvulsant activity of conventional antiepileptic drugs in the mouse maximal electroshock model Trazodone reduces the anticonvulsant action of certain classical antiepileptics in the mouse maximal electroshock model The behavioral effects of the antidepressant tianeptine require the mu-opioid receptor Effect of acute and chronic tianeptine on the action of classical antiepileptics in the mouse maximal electroshock model Isobolographic analysis of antiseizure activity of the GABA type A receptor-modulating synthetic neurosteroids brexanolone and ganaxolone with tiagabine and midazolam Interactions between non-barbiturate injectable anesthetics and conventional antiepileptic drugs in the maximal electroshock test in mice-An isobolographic analysis Pharmacokinetic/pharmacodynamic considerations for epilepsy-depression comorbidities HBK-14 and HBK-15, triple 5-HT 1A , 5-HT 7 and 5-HT 3 antagonists with potent antidepressant-and anxiolytic-like properties, increase seizure threshold in various seizure tests in mice Mirtazapine does not affect pentylenetetrazole-and maximal electroconvulsive shock-induced seizures in mice Possible mechanism of anticonvulsant effect of ketamine in mice Fluoxetine suppresses glutamate-and GABA-mediated neurotransmission by altering SNARE complex Chronic treatment with agomelatine or venlafaxine reduces depolarization-evoked glutamate release from hippocampal synaptosomes Chronic antidepressants reduce depolarization-evoked glutamate release and protein interactions favoring formation of SNARE complex in hippocampus Milnacipran inhibits glutamatergic N-methyl-D-aspartate receptor activity in spinal dorsal horn neurons Open channel block of NMDA receptors by conformationally restricted analogs of milnacipran and their protective effect against NMDA-induced neurotoxicity Inhibition of glutamate release by bupropion in rat cerebral cortex nerve terminals Bupropion attenuates kainic acid-induced seizures and neuronal cell death in rat hippocampus Inhibitory presynaptic 5-hydroxytryptamine(2A) receptors regulate evoked glutamate release from rat cerebellar mossy fibers Tianeptine, an atypical pharmacological approach to depression Involvement of NMDA and AMPA receptors in the antidepressant-like activity of antidepressant drugs in the forced swim test Brain-derived neurotrophic factor and epilepsy: A systematic review BDNF and epilepsy: Too much of a good thing? Valproicacid and epilepsy: From molecular mechanisms to clinical evidences The potential effects of anticonvulsant drugs on neuropeptides and neurotrophins in pentylenetetrazol kindled seizures in the rat Long-term, targeted delivery of GDNF from encapsulated cells is neuroprotective and reduces seizures in the pilocarpine model of epilepsy Regulation of BDNF and trkB mRNA in rat brain by chronic electroconvulsive seizure and antidepressant drug treatments Moclobemide up-regulates proliferation of hippocampal progenitor cells in chronically stressed mice The antidepressant fluoxetine acts on energy balance and leptin sensitivity via Opposite effects of milnacipran, a serotonin norepinephrine reuptake inhibitor, on the levels of nitric oxide and brain-derivedneurotrophicfactor in mouse brain cortex Possible role of Cyclic AMP Response Element Binding/Brain-DerivedNeurotrophicFactor signaling pathway in mediating the pharmacological effects of duloxetine against methamphetamine use-induced cognitive impairment and withdrawal-induced anxiety and depression in rats Obuchowicz, E. The effects of desipramine, fluoxetine, or tianeptine on changes in bulbar BDNF levels induced by chronic social instability stress and inflammation Bupropion monotherapy alters neurotrophic and inflammatory markers in patients of major depressive disorder Antidepressant drug treatments induce glial cell line derived neurotrophic factor (GDNF) synthesis and release in rat C6 glioblastoma cells Antidepressants induce acute CREB phosphorylation and CRE-mediated gene expression in glial cells: A possible contribution to GDNF production Tricyclic antidepressant amitriptyline activates fibroblast growth factor receptor signaling in glial cells: Involvement in glial cell line derived neurotrophic factor production Mirtazapine increases glialcelllinederivedneurotrophicfactor production through lysophosphatidic acid 1 receptor-mediated extracellular signal-regulated kinase signaling in astrocytes Effect of escitalopram on serum GDNF and BDNF levels and 5-HT level of brain tissue of obsessive-compulsive disorder rats Neuroprotective effects of antidepressants via upregulation of neurotrophic factors in the MPTP model of Parkinson's disease Dose translation from animal to human studies revisited Trazodone increases seizures in a genetic WAG/Rij rat model of absence epilepsy while decreasing them in penicillinevoked focal seizure model