key: cord-0721970-ijt9krq5 authors: Matthews, Derek; Atkinson, Robert; Shephard, Adrian title: Spectrum of bactericidal action of amylmetacresol/2,4-dichlorobenzyl alcohol lozenges against oropharyngeal organisms implicated in pharyngitis date: 2018-11-28 journal: Int J Gen Med DOI: 10.2147/ijgm.s184406 sha: 645a3eaa114cc4a4827d02c0ef31b8a19c45b802 doc_id: 721970 cord_uid: ijt9krq5 PURPOSE: Pharyngitis is commonly caused by a self-limiting upper respiratory tract infection (URTI) and symptoms typically include sore throat. Antibiotics are often inappropriately used for the treatment of pharyngitis, which can contribute to antimicrobial resistance, therefore non-antibiotic treatments which have broad antiseptic effects may be more appropriate. Amylmetacresol (AMC) and 2,4-dichlorobenzyl alcohol (DCBA) are present in some antiseptic lozenges and have established benefits in providing symptomatic relief and some in vitro antiviral action. METHODS: Seven bacterial species associated with pharyngitis, namely Streptococcus pyogenes, Fusobacterium necrophorum, Streptococcus dysgalactiae subspecies equisimilis, Moraxella catarrhalis, Haemophilus influenza, Arcanobacterium haemolyticum and Staphylococcus aureus, were exposed to an AMC/DCBA lozenge dissolved in artificial saliva. In vitro bactericidal activity was measured as a log reduction in colony-forming units (CFUs). RESULTS: Bactericidal activity was recorded against all organisms after 1 minute. Greater than 3 log(10) reductions in CFUs were observed at 1 minute for S. pyogenes (log(10) reduction CFU/mL ± SD, 5.7±0.1), H. influenza (6.1±0.1), A. haemolyticum (6.5±0.0) and F. necrophorum (6.5±0.0), at 5 minutes for S. dysgalactiae (6.3±0.0) and M. catarrhalis (5.0±0.9) and at 10 minutes for S. aureus (3.5±0.1). CONCLUSION: An AMC/DCBA lozenge demonstrated a greater than 99.9% reduction in CFUs against all tested species within 10 minutes, which is consistent with the time a lozenge remains in the mouth. Patients with uncomplicated bacterial pharyngitis may benefit from the antibacterial action of antiseptic AMC/DCBA lozenges. Furthermore, AMC/DCBA lozenges may be more relevant and appropriate than antibiotics for pharyngitis associated with a self-limiting viral URTI. Pharyngitis is associated with inflammation of the pharynx 1 and is one of the most common reasons patients seek health care professional advice. 2 Acute pharyngitis is predominantly caused by a viral upper respiratory tract infection such as the common cold 3, 4 and is usually self-limiting with symptoms, such as sore throat, lasting ~3-7 days. 5 Despite this, antibiotics are still frequently inappropriately used for the treatment of pharyngitis even though patients consulting their doctor are often primarily seeking reassurance and symptomatic relief. 6, 7 Antibiotics are ineffective against the viruses that cause ~90% of cases, do not offer symptomatic relief and inappropriate antibiotic prescription can contribute to antimicrobial resistance, which is a serious threat to global public health. 8 Consequently, there is a need for non-antibiotic treatments, 9, 10 which have broad anti-infective effects while meeting patient needs for relief of symptoms. Antiseptics are a class of antimicrobial agent which kill via a physical action on the bacteria. 11 In addition to bactericidal activity, some antiseptics -such as amylmetacresol (AMC) and 2,4-dichlorobenzyl alcohol (DCBA) -have been shown to have antiviral effects in vitro 12, 13 and anesthetic-like effects 14 with established benefits in providing symptomatic relief of pain. 15, 16 Bacterial infections contribute to 5%-15% of pharyngitis cases in adults. 4, 17, 18 The most common bacterial cause of acute pharyngitis, and the reason for legitimate antibiotic prescribing to prevent complications, is group A β-hemolytic Streptococcus (GABHS or Streptococcus pyogenes). 3, 19 It is responsible for ~30% of cases in children 20 and is less frequent in adults at ~10% of cases, 17 but rarely results in complications. 3 A number of other bacteria have also been implicated in infections of the throat, which may present with a more complicated pathology or represent either opportunistic infection or an underlying medical condition. Less common species recovered from patients presenting with symptoms of pharyngitis or with a clinical diagnosis of pharyngitis include Fusobacterium necrophorum, 21 described in a recent study as a true pathogen rather than a colonizer of the oropharynx, 22 and the Streptococcus dysgalactiae subspecies equisimilis, which can cause severe or recurrent pharyngitis, 3, 17, 23 although there is insufficient evidence of a role for S. dysgalactiae in other adverse outcomes. 3 Moraxella catarrhalis has been frequently isolated from patients with pharyngitis in combination with S. pyogenes, 24 which may be significant considering that separate studies have demonstrated that M. catarrhalis potentiates the adhesion of S. pyogenes to the nasopharyngeal epithelium. 25, 26 Other bacteria cultured from patients with pharyngitis include Haemophilus influenza, 27 Arcanobacterium haemolyticum 28 and the opportunistic pathogen, Staphylococcus aureus, although the clinical significance of S. aureus association is not known. 19 In patients diagnosed with tonsillitis, F. necrophorum, appears to be a clinically important species, with a prevalence significantly higher in subjects with clinical tonsillitis compared to subjects without tonsillitis. 29 S. aureus has also been identified as a common cause of tonsillitis 30, 31 and was the most common pathogen isolated from patients undergoing tonsillectomy due to recurrent tonsillitis. 30 H. influenza has similarly been recovered from patients with tonsillitis, although the clinical significance is currently unknown. 27 Non-antibiotic antimicrobial treatments could potentially benefit patients with bacterial pharyngitis by offering not only antimicrobial activity but also symptomatic relief. The in vitro activity of 10 lozenge formulations has previously been investigated against S. pyogenes and S. aureus. 32 In this study, the in vitro bactericidal activity of AMC/DCBA lozenges against a broader range of potentially pathogenic oropharyngeal bacteria was assessed to evaluate the potential in vivo action of these lozenges against organisms associated with pharyngitis. For the bactericidal assay, AMC 0. 6 The bactericidal assay was performed following a protocol similar to the Clinical and Laboratory Standards Institute approved guideline. 33 Specifically, inoculum cultures were prepared for each challenge organism to give an approximate population of 10 8 colony-forming unit (CFU)/mL in saline (0.9% sodium chloride [Fisher Scientific]). One inoculum suspension was prepared for each replicate tested. Test sample (4.9 mL) was prepared as above and inoculated with 0.1 mL of the inoculum suspension. The solution was vortexed thoroughly to mix and then tested after 1-, 5-and 10-minute contact times, consistent with the time a lozenge takes to dissolve in the mouth, 16 For all test organisms, evidence of bactericidal activity was recorded at the 1-minute time point (Table 1 , Figure 1) , and test control counts demonstrated that the test method and media did not affect the survival of the organisms. For S. pyogenes, H. influenza, A. haemolyticum and F. necrophorum, the decrease in CFU/mL at 1 minute exceeded 3 log 10 (99.9% decrease), whereas greater than 3 log 10 reductions were recorded at 5 minutes for S. dysgalactiae and M. catarrhalis and at 10 minutes for S. aureus. Additionally, at all time points, the SD (Table 1 ) of the replicates was small (≤0.9 log 10 CFU/mL), indicating consistent and reproducible observations. The results demonstrated that the AMC/DCBA lozenge exhibits broad bactericidal activity against a range of organisms implicated in pharyngitis and the rapid activity observed is consistent with the time taken for a lozenge to dissolve in the mouth. 16 For all test organisms, evidence of bactericidal activity for the AMC/DCBA lozenge was recorded at the 1-minute time point. Of particular interest is the robust bactericidal activity against S. pyogenes, the most frequent cause of bacterial pharyngitis. 4 Reductions exceeding 99.9% were achieved by 1 minute for S. pyogenes, H. influenza, A. haemolyticum and F. necrophorum, by 5 minutes for S. dysgalactiae and M. catarrhalis and by 10 minutes for S. aureus. The bactericidal activity of an AMC/DCBA lozenge within a 10-minute period is important as it is consistent with the duration that a lozenge remains in the mouth; furthermore, the active ingredients were also tested at the expected concentration achieved when a lozenge is dissolved in the mouth, assuming a volume of 5 mL of saliva. A 33 The data generated in this study support and expand upon these previously published observations, providing further evidence of effectiveness against a broader range of bacterial species under in vitro conditions, including those where knowledge of their clinical pathology in pharyngitis is continuing to evolve or those that represent either an opportunistic infection or an underlying medical condition. These data likewise complement recent studies showing the in vitro viricidal effects of lozenges containing AMC/DCBA (and the active ingredients as free substances) against parainfluenza virus type 3, cytomegalovirus, respiratory syncytial virus, influenza A and severe acute respiratory syndrome coronavirus. 12, 13 In addition to antimicrobial activity, AMC and DCBA are proven to provide relief from the symptoms of pharyngitis, particularly sore throat, likely through their demonstrated local anesthetic-like action against voltage-gated neuronal sodium channels, 14, 34 and therefore may benefit patients presenting with either bacterial or viral pharyngitis. Furthermore, by relieving symptoms and managing patient expectations, the number of instances of inappropriate antibiotic prescribing for viral pharyngitis may be reduced. A limitation of this study is that these observations were performed in vitro and therefore do not fully reflect the environment of the throat. For example, the throat may Bactericidal action of aMc/DcBa lozenges in pharyngitis contain multiple microorganisms whereas this study tested the bactericidal activity against organisms in isolation. The role of the patient's immune system and swallowing action on the antimicrobial activity of the lozenge or active ingredients can also not be determined using in vitro methodology. However, the incidence of these bacteria is relevantly low in the general population; therefore, studying the bactericidal activity of AMC/DCBA in vivo can be challenging. Consequently, an in vitro approach is advantageous allowing the rapid generation of robust data, for multiple organisms simultaneously, that can be used to evaluate the potential of AMC/DCBA for efficacy in vivo. These data show that an AMC/DCBA lozenge demonstrates bactericidal activity against all test organisms, representing a broad range of bacterial cell structures, from 1 minute and achieves greater than 99.9% kill for all test organisms within 10 minutes, which is consistent with the duration that a lozenge remains in the mouth. Therefore, patients with uncomplicated bacterial pharyngitis, including those taking antibiotics, from low-risk populations and without additional risk factors, may benefit from the antiseptic action of AMC/DCBA against a range of bacterial species associated with pharyngitis. Most cases of pharyngitis should not require antibiotics as they are typically self-limiting and often viral in origin. Therefore, over-the-counter antiseptics like AMC/DCBA may be more appropriate, unless the condition deteriorates or a streptococcal infection is diagnosed. All data generated or analyzed during this study are included in this manuscript. submit your manuscript | www.dovepress.com Submit your manuscript here: https://www.dovepress.com/international-journal-of-general-medicine-journal The International Journal of General Medicine is an international, peer-reviewed open-access journal that focuses on general and internal medicine, pathogenesis, epidemiology, diagnosis, monitoring and treatment protocols. The journal is characterized by the rapid reporting of reviews, original research and clinical studies across all disease areas. The manuscript management system is completely online and includes a very quick and fair peer-review system, which is all easy to use. Visit http://www.dovepress.com/testimonials.php to read real quotes from published authors. 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Approved Guideline, CLSI Document M26-A Topical antiseptics for the treatment of sore throat block voltage-gated neuronal sodium channels in a local anaesthetic-like manner The authors would like to thank Aisat Fatade Ogunpola (a former employee of Reckitt Benckiser Healthcare Ltd, UK) for laboratory support. Medical writing assistance was provided by Daniel East at Elements Communications Ltd, Westerham, UK and was funded by Reckitt Benckiser Healthcare Ltd, UK. This work was supported by Reckitt Benckiser Healthcare Ltd, UK. All authors contributed to data analysis, drafting or revising the article, gave final approval of the version to be published, and agree to be accountable for all aspects of the work. Derek Matthews, Robert Atkinson and Adrian Shephard are employees of Reckitt Benckiser Healthcare Ltd, UK. The authors report no other conflicts of interest in this work.