key: cord-0721289-9urotojt
authors: Ritter, Markus; Bresgen, Nikolaus; Kerschbaum, Hubert H.
title: From Pinocytosis to Methuosis—Fluid Consumption as a Risk Factor for Cell Death
date: 2021-06-23
journal: Front Cell Dev Biol
DOI: 10.3389/fcell.2021.651982
sha: 5a6380280cdb46ec5685a26e095e6efc5a5c3f4e
doc_id: 721289
cord_uid: 9urotojt

The volumes of a cell [cell volume (CV)] and its organelles are adjusted by osmoregulatory processes. During pinocytosis, extracellular fluid volume equivalent to its CV is incorporated within an hour and membrane area equivalent to the cell’s surface within 30 min. Since neither fluid uptake nor membrane consumption leads to swelling or shrinkage, cells must be equipped with potent volume regulatory mechanisms. Normally, cells respond to outwardly or inwardly directed osmotic gradients by a volume decrease and increase, respectively, i.e., they shrink or swell but then try to recover their CV. However, when a cell death (CD) pathway is triggered, CV persistently decreases in isotonic conditions in apoptosis and it increases in necrosis. One type of CD associated with cell swelling is due to a dysfunctional pinocytosis. Methuosis, a non-apoptotic CD phenotype, occurs when cells accumulate too much fluid by macropinocytosis. In contrast to functional pinocytosis, in methuosis, macropinosomes neither recycle nor fuse with lysosomes but with each other to form giant vacuoles, which finally cause rupture of the plasma membrane (PM). Understanding methuosis longs for the understanding of the ionic mechanisms of cell volume regulation (CVR) and vesicular volume regulation (VVR). In nascent macropinosomes, ion channels and transporters are derived from the PM. Along trafficking from the PM to the perinuclear area, the equipment of channels and transporters of the vesicle membrane changes by retrieval, addition, and recycling from and back to the PM, causing profound changes in vesicular ion concentrations, acidification, and—most importantly—shrinkage of the macropinosome, which is indispensable for its proper targeting and cargo processing. In this review, we discuss ion and water transport mechanisms with respect to CVR and VVR and with special emphasis on pinocytosis and methuosis. We describe various aspects of the complex mutual interplay between extracellular and intracellular ions and ion gradients, the PM and vesicular membrane, phosphoinositides, monomeric G proteins and their targets, as well as the submembranous cytoskeleton. Our aim is to highlight important cellular mechanisms, components, and processes that may lead to methuotic CD upon their derangement.

The volumes of a cell [cell volume (CV) ] and its organelles are adjusted by osmoregulatory processes. During pinocytosis, extracellular fluid volume equivalent to its CV is incorporated within an hour and membrane area equivalent to the cell's surface within 30 min. Since neither fluid uptake nor membrane consumption leads to swelling or shrinkage, cells must be equipped with potent volume regulatory mechanisms. Normally, cells respond to outwardly or inwardly directed osmotic gradients by a volume decrease and increase, respectively, i.e., they shrink or swell but then try to recover their CV. However, when a cell death (CD) pathway is triggered, CV persistently decreases in isotonic conditions in apoptosis and it increases in necrosis. One type of CD associated with cell swelling is due to a dysfunctional pinocytosis. Methuosis, a non-apoptotic CD phenotype, occurs when cells accumulate too much fluid by macropinocytosis. In contrast to functional pinocytosis, in methuosis, macropinosomes neither recycle nor fuse with lysosomes but with each other to form giant vacuoles, which finally cause rupture of the plasma membrane (PM). Understanding methuosis longs for the understanding of the ionic mechanisms of cell volume regulation (CVR) and vesicular volume regulation (VVR). In nascent macropinosomes, ion channels and transporters are derived from the PM. Along trafficking from the PM to the perinuclear area, the equipment of channels and transporters of the vesicle membrane changes by retrieval, addition, and recycling from and back to the PM, causing profound changes in vesicular ion concentrations, acidification, and-most importantly-shrinkage of the macropinosome, which is indispensable for its proper targeting and cargo processing. In this review, we discuss ion and water transport mechanisms with respect to CVR and VVR and with special emphasis on pinocytosis and methuosis. We describe various aspects of the complex mutual interplay between extracellular and intracellular ions and ion gradients, the PM and vesicular membrane, phosphoinositides, monomeric G proteins and their targets, as well as the submembranous cytoskeleton. Our aim is to highlight important cellular mechanisms, components, and processes that may lead to methuotic CD upon their derangement.

Individual cells have the same need entire organisms have: They have to drink. At the cellular level, water drinking is known as pinocytosis and the fluid-containing organelle is the pinosome. Fluid uptake, intracellular distribution, and processing require precise spatial and temporal coordination of membrane and cytoskeletal proteins. Macropinocytosis is an actin-driven process, where a cup-like structure emerges from the cell surface, which engulfs extracellular fluid and forms a vesicle (Swanson and Watts, 1995; Swanson, 2008; Kerr and Teasdale, 2009; Lim and Gleeson, 2011; Hinze and Boucrot, 2018; Doodnauth et al., 2019; King and Kay, 2019; Swanson and King, 2019) . The vesicle membrane contains ion channels and transporters that are used for the flux of water, osmolytes, and nutrients, and it is decorated by distinct phospholipids and proteins that are required for intracellular sorting and transport of the organelle (Bohdanowicz and Grinstein, 2013; Levin et al., 2015; Freeman and Grinstein, 2018; Williamson and Donaldson, 2019; Stow et al., 2020) ( Table 1) . Macropinocytosis serves seemingly unrelated cellular functions, such as nutrition acquisition to satisfy cellular energy demands (Recouvreux and Commisso, 2017; Palm, 2019; Lin et al., 2020) , immune surveillance leading to antigen presentation to lymphocytes (Lanzavecchia, 1996; Von Delwig et al., 2006; Liu and Roche, 2015; Canton, 2018) , intracellular replication of pathogenic bacteria (Bloomfield and Kay, 2016; Di Russo Case et al., 2016) , and CD by drinking too much fluid, i.e., methuosis Overmeyer, 2014, 2015) . Careful examination of these functions showed similarities in the initial steps of fluid uptake and differences in the final processing steps, such as fusion or not fusion with lysosomes.

The prototypic experiments examining pinocytosis were done on macrophages and malignant cells by Lewis in the 1930s (Lewis, 1936 (Lewis, , 1937 . Then, Lewis was among the few researchers using time-lapse microscopy to study the dynamics of living cells. Lewis introduced the term "pinocytosis" to describe the fact that ruffle formation is associated with vesicle formation and uptake of extracellular fluid (Lewis, 1936 (Lewis, , 1937 . In line with Lewis's statement "Pinocytosis is easily seen in motion pictures" (Lewis, 1937) , we use video microscopy to visualize the dynamics of pinocytosis (Figure 1 and Supplementary  Videos 1, 2) . In the words of Lewis, Supplementary Video 1 shows that vesicles "taken in vary greatly in size, " "several fuse. . . to form larger ones, " "move centrally, " and "finally reach. . . the neighborhood of . . . the nucleus" (Lewis, 1937) . Lewis also 1-phosphatase; INFγ, interferon gamma; INPP5, inositol-polyphosphate 5-phosphatase; INPase, inositol-phosphate phosphatase; INPP, inositol-1,4bisphosphate 1-phosphatase; INPP5, inositol polyphosphate 5 phosphatase; IPPK, inositol-pentakisphosphate 2-kinase; ITPK, inositol 1,3,4-trisphosphate 5/6 kinase, acts also as inositol polyphosphate phosphatase; K v , voltage-gated potassium channel; LPS, lipopolysaccharide; LTD4, leukotriene D4; mAB, monoclonal antibody; MAPK, mitogen-activated protein kinase; M-CSF, macrophage colonystimulating factor; MHC, major histocompatibility complex; MINPP, multiple inositol-polyphosphate phosphatase; MIPP, 3-(2-methyl-1H indol-3-yl)-1-(4pyridinyl)-2-propen-1-one; miR, microRNA; MKK4, mitogen-activated protein kinase kinase 4; MLC1, megalencephalic leukoencephalopathy with subcortical cysts 1; MOMIPP, 3-(5-methoxy, 2-methyl-1H-indol-3-yl)-1-(4-pyridinyl)-2propen-1-one; MTM1, myotubularin 1; MTMR, myotubularin-related protein; MTMR6, myotubularin R6; mTOR, mammalian target of rapamycin; MVB, multi-vesicular body; NAADP, nicotinic acid adenine dinucleotide phosphate; NCX, Na + /Ca 2+ -exchanger; NFAT5, nuclear factor of activated T cells 5; NGF, nerve growth factor; NOSTRIN, nitric oxide synthase trafficking inducer; NRF2, nuclear factor erythroid 2-related factor 2; NVI, necrotic volume increase; OCR, Lowe oculocerebrorenal syndrome protein; Orai, named after the keepers of the gates of heaven in Greek mythology, synonym: CRACM, calcium release-activated calcium channel protein; OMIM, Online Mendelian Inheritance in Man R ; Ostm1, osteoclastogenesis-associated transmembrane protein; p53, tumor protein P53 or cellular tumor antigen p53; Pak, p21-activated kinase; Panx1, pannexin 1; PDGF, platelet-derived growth factor; PDK1, 3-phosphoinositide-dependent protein kinase 1; PEG, polyethyleneglycol; Pf, osmotic water permeability; PFKFB3, 6-phosphofructo-2-kinase/fructose-2,6 biphosphatase 3; PH, pleckstrin homology; pH i , intracellular pH; PI, phosphoinositide or phosphatidylinositol; PI(3)P, phosphatidylinositol 3-phosphate; PI(3,4,5)P3, phosphatidylinositol (3,4,5)-trisphosphate; PI(4,5)P2, phosphatidylinositol 4,5-bisphosphate; PI3K, phosphatidylinositol 3 kinase; PIKfyve, phosphoinositide kinase FYVE-type zinc finger containing; PKB, protein kinase B; PLA2, phospholipase A2; PLC, phospholipase C; PM, plasma membrane; PS, phosphatidylserine; PRR, pattern recognition receptor; PTEN, phosphatase and tensin homolog; P2X-R, ATPgated P2X receptor cation channel; RA, retinoic acid; Rab, Ras-related in brain; Rac, Ras-related C3 botulinum toxin substrate; RACK1, receptor for activated C kinase 1; Raf1, rapidly accelerated fibrosarcoma 1; Rap1, Ras-proximate-1/Ras-related protein 1; Ras, rat sarcoma; Rasal, Ras-GTPase-activating-like protein; RGBARG, RCC1-RhoGEF-BAR-and-RasGAP-containing protein; Rheb, Ras homolog enriched in brain; Rho, Ras homologous; RIN1, Ras and Rab interactor 1; RN-tre, Related to the N-terminus of tre; ROS, reactive oxygen species; RVD, regulatory volume decrease; RVI, regulatory volume increase; SGK, serum-and glucocorticoid-inducible protein kinase; SLC, solute carrier; SMIT, Na + /myo-inositol transporter; SOCE, store-operated calcium entry; STIM1, stromal interaction molecule 1; TFEB, transcription factor EB; TMEM, transmembrane protein; TonEBP, tonicity responsive enhancer (TonE) binding protein; TORC, target of rapamycin complex; TPC, two-pore channel; TRP, transient receptor potential; TRPML, TRP channel of mucolipin subfamily; TRPM7, Transient receptor potential cation channel subfamily M member 7; TSC, tuberous sclerosis complex; Ttyh, Tweety homolog; v-ATPase, vacuolar H + -ATPase; Vps34, vacuolar protein sorting 34; VSOR, volume-sensitive outwardly rectifying Cl − channel/current; VRAC, volume-regulated anion channel/current; VVR, vesicular volume regulation; WASP, Wiskott-Aldrich syndrome protein; WNK, with no lysine kinase Frontiers in Cell and Developmental Biology | www.frontiersin.org References: (Sonawane and Verkman, 2003; Dong et al., 2010; Morgan et al., 2011; Scott and Gruenberg, 2011; Welliver and Swanson, 2012; Bohdanowicz and Grinstein, 2013; Ishida et al., 2013; Stauber and Jentsch, 2013; Chang et al., 2014; Egami et al., 2014; Levin et al., 2015; Saha et al., 2015; Xu and Ren, 2015; Xiong and Zhu, 2016; Chakraborty et al., 2017; Kim et al., 2018; Sterea et al., 2018; King and Kay, 2019; Li P. et al., 2019; Adjemian et al., 2020; Jin et al., 2020; Trivedi et al., 2020; Chadwick et al., 2021b; Riess et al., 2021; Zhang et al., 2021) .

FIGURE 1 | Macropinocytosis occurring in a section of a multinucleated giant cell from a rat non-parenchymal hepatic cell line putatively representing immortalized monocytes (Kupffer cells). The image series shows a section of a giant cell where pinocytosis occurs. The process starts with the formation of a membrane ruffle at minute 2 (2 ; arrow) from which an array of vesicles (pinosomes) originates (3 -4 ) . Further ruffling can be seen at the cell periphery (2 ; arrowhead). A second ruffle is forming at minute 5 (5 ; arrow) from which additional pinosomes derive (5 -7 ). The whole pinosome array subsequently moves toward the center area of the giant cell (8 -18 ) locating to the vicinity of a nucleus (N in 18 ) . Terminally, the vesicles start to disappear in the perinuclear (pericentral) cytosol (20 ) . Scale bar = 10 µm. The whole live cell imaging sequence can be seen in Supplementary Video 1.

Frontiers in Cell and Developmental Biology | www.frontiersin.org suggested that proteins in the vesicles are "split by the digestive enzymes into simpler products which can be utilized or can diffuse out of the cell." He also related the "disappearance" of the vesicles "with the completion of the digestion of their contents" as the vesicles "slowly shrink in size and disappear, leaving a small granule. . ." (Lewis, 1937 ). Lewis's experiments also showed that the cells do not increase in volume, although "they may take in several times their volume of fluid" and he "assumed that the fluid diffuses out of the cells when the globules disappear" (Lewis, 1937) . These experiments demonstrate that volume regulatory processes at the cellular and organelle levels are of paramount importance to maintain CV while cells incorporate large volumes of extracellular fluid and digest macromolecules present in the fluid. The fact that the fluid taken up by macropinocytosis outweighs its elimination by recycling vesicles (Swanson, 1989; Choy et al., 2018) highlights that VVR and CVR are inevitably linked to each other. This becomes evident from the massive cell swelling seen upon inhibition of water channels in pinocytosing cells (De Baey and Lanzavecchia, 2000) . Furthermore, considering that the total volume of the endolysosomal compartment can make up a substantial part of the total CV (Choy et al., 2018) makes evident that the exchange of osmotically active solutes between the endolysosomal compartment and the cytosol will strictly affect the volumes of either part. Given the importance of CVR, a central question is: What determines the size of a vesicle? That is, which transporters and ion channels in the PM and vesicle membrane are activated, incorporated, and terminated at which spatial and temporal check points? How does the macropinosomal solute composition and the vesicular membrane properties change after having gulped a lot of extracellular fluid during maturation along the endolysosomal pathway, and what are the determinants of this change? And finally: How are these cellular and subcellular volume regulatory mechanisms altered in methuosis?

To understand subcellular volume regulation, findings related to CVR provide clues to identify factors maintaining the set points of the vesicle.

As ion channels and transporters in the PM are central in CVR, they also contribute to VVR (Freeman and Grinstein, 2018; Freeman et al., 2020; Chadwick et al., 2021b) . Thus, Lewis's statement in the 1930s that "The factors involved in the diffusion of the fluid out of the cell are as mysterious as most of the other processes which take place" (Lewis, 1937) is now transforming to hypotheses trying to explain CVR and VVR in macropinocytosis at the molecular level and by facts generated by electrophysiological, molecular-biological, and imaging studies. Notably, Freeman and Grinstein (2018); Freeman et al. (2020), and Chadwick et al. (2021b) demonstrated that contributions from ion transporters are essential for normal shrinkage in macropinosome maturation.

This review focuses on the complex mutual interplay between extracellular and intracellular ions and ion gradients, the PM and vesicular membrane, phosphoinositides, monomeric G proteins and their targets, as well as the submembranous cytoskeleton in pinocytosis, with special emphasis on its connection to CVR and VVR. It aims at highlighting important cellular mechanisms and components that govern these processes and that may lead to methuotic cell death upon their derangement. Figure 2 schematically shows key steps of vesicle/vacuole formation and processing during normal macropinocytosis and in methuosis.

Macropinocytosis is a form of clathrin-independent endocytosis. Ruffling of cholesterol-rich membrane microdomains leads to the unselective incorporation of large volumes of extracellular fluid in macropinosomes with a diameter of 0.2 up to 5.0 µm (Levin et al., 2015; Donaldson, 2019) . Macropinocytosis may occur constitutively or be induced by growth factors, chemokines, microbial products, viruses Marques et al., 2017; Canton, 2018; Doodnauth et al., 2019; Tejeda-Munoz et al., 2019) , crosslinking of cell surface molecules (Imamura et al., 2011) , knockdown of genes (Choi et al., 2017; Fomin et al., 2018; Fomin, 2019; Su et al., 2021) , and constitutive expression (Kasahara et al., 2007) or mutations of signal transduction molecules (Yoo et al., 2020) . Some cells, like innate immune cells and Ras-transformed cancer cells, are able to perform both forms of macropinocytosis (Amyere et al., 2000; Stow et al., 2020) . Importantly, this process ensures that cells incorporate everything animals ingest and digest, including nutrients as well as toxic substances and metabolites released by neighboring cells, exosomes, microparticles, and pathogens, such as bacteria and viruses (Suda et al., 2007; Helenius, 2009, 2012; Mercer et al., 2010; Commisso et al., 2013; Bloomfield and Kay, 2016; Jiang et al., 2017; Commisso, 2019) . Furthermore, macropinocytosis is involved in cell migration (Wen et al., 2016; Swanson and King, 2019) .

Remarkably, within 1 h, a volume equivalent of the entire cytoplasm is incorporated by macropinocytosis, and within 30 min, macropinosome formation requires the entire cellular PM surface (Steinman et al., 1976; Cullen and Steinberg, 2018; Freeman and Grinstein, 2018) . Against the compelling background of conserved CV and cell surface, sorting mechanisms distinguishing between recycling and digestion routes are of eminent importance. Membrane recycling is not only of importance for maintaining the cell surface but also for supplying the PM with receptors and transporters, such as neonatal Fc-receptor (Toh et al., 2019) , bone morphogenetic protein receptor , PDGF β-receptor (Schmees et al., 2012) , EGF receptor (Chiasson-Mackenzie et al., 2018; Freeman et al., 2020) , and other plasmalemmal components such as integrins (Buckley et al., 2016; Freeman et al., 2020) as well as small GTPases, which fuel macropinocytosis (Cullen and Steinberg, 2018) . Furthermore, as the intercellular volume is usually small, changes in extracellular ion composition may greatly affect ion gradients, which drive nutrient transporters, e.g., Na + -dependent glucose or amino acid uptake (Ganapathy et al., 2008; Wright et al., 2011; Broer, 2014) . This exceptional well-balanced system is keeping CV and cell surface during macropinocytic flow reasonably constant, but puts the cell at FIGURE 2 | Simplified scheme of key processes in vesicle/vacuole formation and processing during normal macropinocytosis and in methuosis. Macropinocytosis is an actin-driven process that is triggered by various stimuli. It starts with ruffling of the plasma membrane (PM) and formation of a pinocytotic cup, which engulfs extracellular fluid and forms a pinosome by membrane fusion at the tip of a lamellipodium-like structure. Its membrane contains ion channels and transporters, receptors, among other various PM constituents, e.g., phospholipids. The nascent pinosome unselectively engulfs extracellular fluid, along with its ions, nutrients, and metabolites and eventually also toxins or drugs. It may also enclose particulate matter-like exosomes, micro-or nanoparticles, or pathogens such as bacteria and viruses (pink enclosed structure). Under normal conditions (green vesicles), pinosomes move centripetally, become more and more acidic, shrink along their route, and become tubulated, a process necessary for proper sorting and recycling of the vesicles and their cargo. This requires also its decoration with distinct phospholipids and proteins (not shown). Reusable membrane proteins may become inserted again into the PM when vesicles fuse with it. This also recycles incorporated membrane back to the PM and relieves its tension. Vesicles designated for delivery of their contents to lysosomes-for further processing, digestion, or destruction-fuse with them and finally resolve. The resulting products may be further used, e.g., for the cell's nutrition. To ensure these processes, cell volume regulation and vesicular volume regulation must work hand in hand. This becomes evident from the fact that during pinocytosis, an extracellular fluid volume and membrane area equivalent to the cell's volume and to the cell's surface are incorporated within 1 h and 30 min, respectively. The volume regulatory mechanisms involve movement of ions and osmolytes across the PM by means of specific ion channels and transporters. The driving forces for these fluxes are determined by the electrochemical gradients. Water flux is driven mainly by the osmotic ( ) but eventually also by the hydrostatic ( P) pressure differences. The water permeability of the PM is greatly enhanced by water channels (aquaporins, blue). All of these movements are primarily driven by the activity of the Na + /K + -ATPase (red transporter in the PM), an ion pump that moves Na + ions out of and K + ions into the cell, while hydrolyzing ATP as energy source. This process sets up the required ionic and osmotic gradients and determines the electrical potential difference across the PM. The mechanisms for vesicular volume regulation follow the same rules and are in principle identical to those in cell volume regulation, while utilizing their individual set of transporters and channels. In methuosis, a lethal process of aberrant pinocytosis where cells "drink themselves to death," these processes are severely disturbed (brown vesicles). Fluid uptake by macropinocytosis is enhanced, and instead of shrinking, the vesicles swell, do not acidify, remain non-functional, and do not fuse with lysosomes, but instead they with each other. This leads to the formation of giant vacuoles, catastrophic cell swelling, and consequently to rupture of the PM, cell lysis, and death. risk to damage either when CVR fails or when it accumulates metabolic waste products as seen in lysosomal storage diseases (Platt et al., 2012 (Platt et al., , 2018 Rappaport et al., 2016) . Excessive fluid uptake in in vitro conditions leading to a distinct form of CD has been recently recognized and named methuosis Overmeyer, 2014, 2015) .

Ruffle and cup formation is an actin-driven process, which is closely related to the formation of phagocytic cups and pseudopods (Lim and Gleeson, 2011; Freeman and Grinstein, 2014; Buckley and King, 2017; Williamson and Donaldson, 2019) . Actin cytoskeleton rearrangement depends on phospholipids, lipid kinases and phosphatases, small GTPases, actin-modulating proteins, ion channels, and proton (H + ) pumps (Welliver and Swanson, 2012) . Among the numerous small GTPases, the Ras and Rho family member, Rac1, is critical in the formation of ruffles and macropinocytic cups (Egami et al., 2014; Donaldson, 2019) . For small GTPases, the switch from an inactive guanosine diphosphate (GDP)-bound form to an active guanosine triphosphate (GTP)-bound form is facilitated by GEFs, which promote GDP dissociation. The inactivation of the small GTPases is mediated by GTPase-activating proteins (GAPs), which enhance GTP hydrolysis (Cherfils and Zeghouf, 2013) . Critically, the activation of the GTPase cycle-activation, inactivation, removal from the membraneis transient. Oscillations of Ca 2+ i may drive parallel oscillatory association and dissociation of the Ras-GAP, Rasal (Ras-GTPaseactivating-like protein), to and from the PM. Only when bound to the PM, Rasal is active and can inactivate Ras. Thus, Ras is repetitively activated and inactivated (Walker et al., 2004) . In macropinocytosis, Ras activity is terminated by RasGAP, which is recruited to the cup as it closes (Veltman et al., 2016; Buckley et al., 2020) . When GTPases are persistently activated, e.g., when RasGAPs are inactive like in neurofibromatosis (Bloomfield et al., 2015; Ghoshal et al., 2019) or when Ras is constitutively active like in oncogenic H-Ras mutants, macropinosome formation and maturation deviate from the normal physiological pathway. The Ras-related G protein Rap1 is a negative regulator of Ras (Zwartkruis and Bos, 1999; Nussinov et al., 2020) . Rap1 is found in early and late endocytic vesicles and lysosomes (Pizon et al., 1994) , and its overexpression negatively regulates macropinocytosis (Seastone et al., 1999) . In Dictyostelium discoideum, hyperosmotic stress activates Rap1 and decreases endocytic activity due to cellular acidification (Pintsch et al., 2001) , and it promotes the formation of giant vacuoles of pinocytotic origin (Yuan and Chia, 2001) .

Notably, in human intestinal cells, hypotonicity increases the activity of the H-Ras-Raf1-Erk signaling pathway (Van Der Wijk et al., 1998) . The clustering of Ras proteins in distinct microdomains at the PM (lipid rafts) influences Ras structure, orientation, and Ras-isoform accessibility and thus the activation states of its effectors. Accordingly, GTP-bound H-Ras may remain in a locked state and as such not able to associate with its downstream effectors (Jang et al., 2016; Nussinov et al., 2018) . Activation occurs once the lipid-anchored GTPases Ras1 and H-Ras are shifted out of the lipid rafts. This happens upon thinning of the PM in combination with changes of its curvature induced by cell swelling (Cohen, 2018) .

Active Rac and Ras are located at the cup wall. Rac1 activation is associated with ruffle formation, and Rac1 inactivation precedes cup closure (Buckley and King, 2017) . In Dictyostelium discoideum, the multidomain protein, RGBARG (RCC1, RhoGEF, BAR, and RasGAP-containing protein), orchestrates Ras and Rac activity in a small membrane patch, where RGBARG is localized at the protruding rim region . Oncogenic H-Ras promotes the translocation of the vacuolar H + -ATPase (v-ATPase) from intracellular vesicles to the PM. The accumulation of v-ATPase is necessary for the cholesterol-dependent association of Rac1 with the PM, which is a prerequisite for the stimulation of membrane ruffling and macropinocytosis. Knockdown of the v-ATPase or its inhibition suppresses macropinocytosis, while addition of cholesterol to these cells restores both Rac1 membrane localization and macropinocytosis (Ramirez et al., 2019) . In addition, Ras binds and activates phosphatidylinositol 3-kinases (PI3Ks), which have a Ras-binding domain (Gupta et al., 2007; Castellano and Downward, 2011; Castellano et al., 2013) . Activation of PI3Ks phosphorylates phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] to phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and promotes its enrichment in the PM (Rupper et al., 2001; Nussinov et al., 2020) . In EGF-stimulated A431 cells, PI(4,5)P2 increases in the ruffles-forming macropinocytic cups, reaches its maximum just before macropinosome closure, and then rapidly falls as the cup closes. In contrast, PI(3,4,5)P3 increases locally at the site of macropinosome formation and peaks at the time of closure (Araki et al., 2007) . The extension of PI(3,4,5)P3 patches, which can reach a diameter of several micrometers, reflects the balanced activity of PI3Ks and the lipid phosphatase, phosphatase and tensin homolog (PTEN), which dephosphorylates PI(3,4,5)P3 back to PI(4,5)P2. The kinetics of PI(4,5)P2 and PI(3,4,5)P3 are mechanistically linked to actin-remodeling during macropinocytosis (Araki et al., 1996 (Araki et al., , 2007 Worby and Dixon, 2014; Swanson and Yoshida, 2019; Nussinov et al., 2020) and to the regulation of many ion channels and transporters relevant to pinocytosis as well as CVR (Araki et al., 1996; Lang et al., 1998; Ritter et al., 2001; Furst et al., 2002; Jakab et al., 2002; Okada, 2004; Lang, 2007; Suh and Hille, 2008; Hoffmann et al., 2009; Abu Jawdeh et al., 2011; Lang and Hoffmann, 2012; Balla, 2013; Hansen, 2015; Hille et al., 2015; Kunzelmann, 2015; Jentsch, 2016; Pasantes-Morales, 2016; De Los Heros et al., 2018; Delpire and Gagnon, 2018; Konig et al., 2019; Okada et al., 2019; Centeio et al., 2020; Larsen and Hoffmann, 2020; Model et al., 2020) . For cup closure, the progressive dephosphorylation of PI(3,4,5)P3 seems to be important. Among its dephosphorylation products, PI(3)P has been shown to directly activate the Ca 2+ -activated K + -channel, KCa3.1, at ruffles, which is necessary for closure . The significance of Ras activation, which peaks shortly after cup closure (Welliver and Swanson, 2012; Egami et al., 2014) , as well as of the antagonistic behavior of PI3Ks and PTEN in the initiation and termination of cup formation is nicely documented by the observations that injection of Ras in cells induces ruffle formation, that inhibitors of PI3K inhibit cup closure in macrophages, and that deletion of PTEN in prostate cancer cells enhances macropinocytosis (Bohdanowicz and Grinstein, 2013; Levin et al., 2015; Kim et al., 2018; King and Kay, 2019) .

Phosphatidylinositol (3,4,5)-trisphosphate enrichment promotes the translocation of the serine/threonine protein kinase Akt/PKB via binding to the pleckstrin homology (PH) domain and kinase activation by target of rapamycin complex 2 (TORC2) and 3-phosphoinositide-dependent protein kinase-1 (PDK1) (Yoshida et al., 2018; . Among the downstream targets of Akt is the tuberous sclerosis complex 2 (TSC2), which is-together with TSC1-located on the lysosomal membrane, from which it subsequently dissociates to act as a GAP for the Ras-related small GTPase, Rheb, which in turn activates mammalian target of rapamycin complex 1 (mTORC1). In Dictyostelium discoideum downstream of PI(3,4,5)P3, the homologs of mammalian Akt, PkbA, and of the related glucocorticoid-regulated kinase (SGK), PkbR1, as well as their activating protein kinases, TORC2 and PdkA, increase the size of the macropinocytic patch and macropinosome Williams et al., 2019) . Combined inhibition of mTORC1/mTORC2 induces massive catastrophic macropinocytosis, i.e., methuosis, in cancer cells (Srivastava et al., 2019) . Akt and TORC2 target SGK1, which regulates a plentitude of cell functions, including ion channels and transporters (Lang et al., 2018) and endomembrane trafficking . SGK transcription is stimulated by cell shrinkage via p38-kinase and inhibited by cell swelling due to transcriptional stop (Waldegger et al., 1997 (Waldegger et al., , 2000 Lang et al., 2006 Lang et al., , 2018 . Furthermore, the isoform Akt3 controls actindependent macropinocytosis in macrophages by suppressing the expression of with no lysine kinase 2 (WNK2) and the activity of SGK1, while increased activity of SGK1 leads to stimulation of Cdc42-mediated macropinocytosis of lipoprotein (Ding et al., 2017) . However, in macrophages stimulated by macrophage colony-stimulating factor (M-CSF), the Akt pathway is induced by this growth factor but not required for macropinosome formation (Yoshida et al., 2015) .

In myoblasts, an acute decrease of PM tension leads to phospholipase D2 activation, production of phosphatidic acid, F-actin and development of PI(4,5)P2-enriched membrane ruffling, and macropinocytosis without an increase in PI(3,4,5)P3 (Loh et al., 2019) .

In parallel to the lipid and protein phosphorylation cascades, the cortical actin filaments are reorganized. This is controlled by PI3K and PLC, Rac, Cdc42, Arf6, Rab5, and Pak (Swanson, 2008) . In the cup region, polymerized actin is seen as a ring-like structure (Hinze and Boucrot, 2018) . Active Ras and PIP(3,4,5)P3 coincidentally form patches in macropinosomes, which are surrounded by a ring of the Scar/WAVE complex, an activator of the Arp2/3 complex. Arp2/3 drives actin polymerization by filament branching, leading to the formation of dendritic F-actin, which populates the wall of the cup and forms rings of protrusive actin under the PM and the circular ruffles (Swanson, 2008; Saarikangas et al., 2010; Pollard, 2016; Buckley and King, 2017; Hinze and Boucrot, 2018; Williamson and Donaldson, 2019) . PI(3,4,5)P3 also recruits myosin proteins to macropinocytic cups (Chen et al., 2012) . The synthesis of PI(3,4,5)P3 from PI(4,5)P2 occurs simultaneously with the recruitment of Rab5 to the PM in COS-7 cells expressing oncogenic H-Ras G12V (Porat-Shliom et al., 2008) . Rab5 promotes macropinosome sealing and scission downstream of ruffling. To this end, Rab5-containing vesicles are recruited to circular ruffles of the PM, which requires soluble N-ethylmaleimide-sensitivefactor attachment receptor (SNARE)-dependent endomembrane fusion. This is paralleled by the disappearance of PI(4,5)P2 and accompanies macropinosome closure. The removal of PI(4,5)P2 is dependent on Rab5 through its recruitment of the inositol 5-phosphatase Inpp5b/OCRL and via APPL1, an adaptor protein that regulates vesicle trafficking and endosomal signaling (Maxson et al., 2021) . Rab5 and RN-tre, which is a Rab5-specific GAP as well as a Rab5 effector, are also recruited to the PM. RN-tre interacts with actin as well as actinin-4, which contributes to actin bundling (Lanzetti et al., 2004) . The Rab5 cycle is active on nascent macropinosomes and stabilizes the macropinosomes. Rab5 activity is increased on macropinosome tubules (Feliciano et al., 2011) . In BHK-21 cells, H-Ras G12V has been shown to separately activate Rab5 and Rac1 via distinct Ras signal transduction pathways. While Rab5 activation stimulates pinocytosis, Rac1 stimulation causes membrane ruffling but does not contribute to the stimulation of pinocytosis (Li et al., 1997) .

In Dictyostelium, the nascent vesicles lose their actin coat within 1 min after pinching off and internalization (Lee and Knecht, 2002) . In rat basophilic leukemia (RBL) cells, "pinosomes. . . ignite a burst of actin polymerization when they are pinched off from the plasma membrane" and "then move into the cytosol at the tips of short-lived actin 'comet tails,' " which fade within 2 min after their appearance. These brief bursts of actin polymerization are thought to help move the vesicles into the cytosol (Merrifield et al., 1999) . They also require recruitment of annexin-2 to nascent macropinosome membranes as an essential prerequisite for actin polymerization-dependent vesicle locomotion (Merrifield et al., 2001) .

Organelle shrinkage concentrates the to-be-digested material and recycles membrane back to the PM. Macropinosomes show two routes of structural adaptations to maximize the organelle surface area and to minimize its volume: tubulation and shrinkage (Freeman and Grinstein, 2018; King and Kay, 2019; Chadwick et al., 2021b) . Macropinosomes retrieve v-ATPase from fusion with other vesicles and mature toward acidic organelles that contain hydrolytic enzymes, such as proteases, nucleases, and lipases, which are required for the degeneration of macromolecules, as well as transporters facilitating the efflux of cholesterol, cystine (the disulfide form of cysteine, which is generated during protein degradation), amino acids, cobalamin, and inorganic ions (Neuhaus et al., 2002; Buckley and King, 2017; Trivedi et al., 2020) .

Mobilization and maturation of macropinosomes depend on the decoration of the vesicle membrane with distinct small GTPases (Egami et al., 2014; Egami, 2016) . Transient activation of ADP-ribosylation factor 6 (Arf6), a member of the Ras superfamily, by the exchange factor, EFA6, promotes PM protrusion, formation of macropinosomes, and recycling of the vesicle membrane back to the PM (Brown et al., 2001) . Arf6 colocalizes and activates phosphatidylinositol 4-phosphate 5-kinase (PIP 5-kinase), which phosphorylates phosphatidylinositol 4-phosphate PI(4)P to PI(4,5)P2 (Egami et al., 2014) . Subsequently, PI(4,5)P2 and Cdc42-GTP coordinate the activation of Wiskott-Aldrich syndrome protein (WASP), which promotes the actin-nucleating and actin filament-branching activity of Arp2/3 (Higgs and Pollard, 2000) . The recruitment of Arf6 and its exchange factor, ARF nucleotide binding-site opener (ARNO), from cytosol to endosomal membranes relies on v-ATPase-dependent intra-endosomal acidification, thus regulating the proteindegradative pathway (Hurtado-Lorenzo et al., 2006) . Persistent activation of Arf6, as seen in the GTP hydrolysis-resistant mutant Arf6 Q67L or by overexpression of the PIP 5-kinase, results in the accumulation of macropinosomes. Interestingly, PI(4,5)P2-enriched macropinosomes in apposition to each other fuse with one another and give rise to large vacuoles. Furthermore, entrapped PM proteins in these vacuoles are not recycled (Brown et al., 2001) . The GTPase septin is involved in endosome fusion. Whereas septin downregulation decreases macropinosome fusion events as well as lysosomal delivery, septin overexpression increases delivery to lysosomes (Dolat and Spiliotis, 2016) .

Early macropinosomes harbor Rab5 (Feliciano et al., 2011; Maxson et al., 2021) , which in turn recruits the class III PI3K Vps34, which catalyzes the reaction from PI to PI(3)P (Christoforidis et al., 1999; Kerr and Teasdale, 2009 ). In macropinosomes routed toward lysosomes, Rab5 is replaced by Rab7 (Racoosin and Swanson, 1993; Kerr et al., 2006; Langemeyer et al., 2018; Morishita et al., 2019) . In an analogy to an electrical safety-breaker, Del Conte-Zerial et al. (2008) compare the replacement of Rab5 by Rab7 as a "cutout switch." This model predicts that Rab5 drives Rab7 activation above a distinct threshold. Above the threshold, Rab7 shows a self-sustained activity and suppresses Rab5 activity via a negative feedback. In this model, crossing the Rab7 activation threshold excludes reactivation of Rab5 and activation of a different trafficking pathway. Thus, the Rab5 to Rab7 switch ensures a unidirectional route of cargo-loaded macropinosomes toward lysosomes. Using Förster/fluorescence resonance energy transfer (FRET) imaging, Morishita et al. (2019) describe that active Rab5 facilitates Rab7 activation until Rab7 sustains its own activity and inactivates Rab5. Furthermore, recruitment of amyotrophic lateral sclerosis 2 (ALS2) to the macropinosome coincides with Rab5 activation, and ALS2 detachment is associated with Rab5 inactivation (Morishita et al., 2019) . In earlier studies using Texas redlabeled dextran macropinosomes, Racoosin and Swanson showed that Rab7-positive macropinosomes fuse with tubular lysosomes (Racoosin and Swanson, 1993) . The fate of waste-containing vesicles is not known.

Dysfunction or inhibition of Vps34 or PIKfyve, which phosphorylates PI(3)P to PI(3,5)P2, leads to the formation of massive and progressively exacerbating cytoplasmic vacuolization due to loss of PI(3,5)P2. This requires active v-ATPase activity and a functional Rab5a cycle. Interestingly, the formation of the enlarged vacuoles does not require their acidification (Compton et al., 2016; Saveanu and Lotersztajn, 2016) , pointing to an osmotic function of the v-ATPase. In melanoma cells, oncogenic class I PI3K elicits a hyperactive influx of macropinosomes, which is counteracted by Rab7A (Alonso-Curbelo et al., 2015) . Furthermore, by stimulating RIN1, which is a Rab5 GEF, activation of H-Ras or H-Ras G12V also mediates homotypic fusion of early endosomes, thus leading to endosome enlargement (Roberts et al., 2000; Tall et al., 2001) .

Microtubules are associated with peripheral actin/myosinenriched lamellae, and they are the scaffold for the unidirectional transport of macropinosomes. Critically, inhibitors of microtubule assembly, and the dynein inhibitor ciliobrevin D, decrease fluid uptake, indicating that the microtubules are involved in an early step of macropinocytosis (Williamson and Donaldson, 2019) .

In general, cells respond to alterations of the osmotic equilibrium with a change of their volume due to water movement into or out of the cell. While an increase in intracellular or a decrease in extracellular osmolarity leads to cell swelling, a decrease in intracellular or an increase in extracellular osmolarity leads to cell shrinkage. Physiologically, such changes arise when cells invade anisotonic extracellular environments, e.g., the renal medulla, or following changes in intracellular osmolyte concentrations, such as during uptake or release of ions or nutrients, but also from metabolic changes, such as formation or degradation of macromolecules, e.g., proteins or glycogen. Likewise, perturbations of the double Donnan equilibrium, established by the so-called pump-leak balance (Okada, 2004; Kay, 2017; Kay and Blaustein, 2019) , such as changes in intracellular pH (pH i ) or inhibition of the Na + /K + -ATPase by cardiac glycosides or low temperature (Russo et al., 2015) , will lead to alterations of CV (for review, see Lang et al., 1998; Ritter et al., 2001; Furst et al., 2002; Jakab et al., 2002; Okada, 2004 Okada, , 2020 Lang, 2007; Hoffmann et al., 2009; Kunzelmann, 2015; Jentsch, 2016; Pasantes-Morales, 2016; De Los Heros et al., 2018; Delpire and Gagnon, 2018; Okada et al., 2019; Centeio et al., 2020; Larsen and Hoffmann, 2020; Model et al., 2020) .

The kinetics and degree of the actual volume changes critically depend on the water permeability of the PM, which is intrinsically low but greatly enhanced by aquaporins (AQPs) (Day et al., 2014; Kitchen et al., 2015) and also by the efficiency as well as the time of onset of CVR mechanisms. If the regulatory mechanisms [i.e., regulatory volume decrease (RVD) and regulatory volume increase (RVI); see below] have high transport efficiency and/or will start to work quickly, the degree of swelling or shrinkage will deviate from a perfect osmometer-like behavior.

When the actual CV deviates from the given set point, regulatory mechanisms are spurred, aiming at readjusting the original volume. Thus, upon swelling, cells initiate a process termed RVD (cells shrink back toward their original volume), while cell shrinkage is counteracted by RVI (cells swell back toward their original volume). The mechanisms driving the regulatory water fluxes during RVD and RVI are complex and involve rapid release or uptake of ions, amino acids, sugars, or alcohols across the cell membrane but also metabolic changes such as the formation or degradation of macromolecules to pack or unpack abundant osmotically active solutes.

Acute CVR relies on distinct sets of ion channels and transporters. The Na + /K + -ATPase actively pumps K + into and Na + out of the cell. K + permeating through K + channels generates a negative PM potential PM and thus creates the driving force for the cellular exit of anions such as Cl − and HCO 3 − . Canonically, during RVD, volume-sensitive K + and anion channels, KCl cotransport, or parallel activation of K + /H + exchange and Cl − /HCO 3 − exchange is activated, while during RVI, Na + /H + exchangers (NHEs) and Na + /K + /2Cl − cotransporters (NKCCs) in parallel to Cl − /HCO 3 − exchange or Na + channels are activated to release or take up ions and osmotically obliged water through AQPs. Thus, RVD is mainly accomplished via cellular exit of K + , Cl − , and HCO 3 − , whereas RVI is achieved by uptake of Na + and Cl − . Frequently, RVD mechanisms are inhibited during RVI and vice versa. Furthermore, shrunken cells can accumulate organic osmolytes such as amino acids, myoinositol, betaine, and taurine either by synthesis or by Na + -coupled uptake of sorbitol, glycerophosphorylcholine, and monomeric amino acids. These osmolytes are then released upon cell swelling. Though inhibition of the Na + /K + -ATPase leads to cell swelling at least in some cells (Alvarez-Leefmans et al., 1992) , interestingly, in certain cell types, CVR can also be performed by formation and exocytosis of vesicles even when the Na + /K + -ATPase is inhibited (Russo et al., 2015) .

Cell volume also greatly affects macromolecular crowding, i.e., the concentration of macromolecules (mainly proteins and nucleic acids) within the cell and hence their biological activities, which in turn has widespread consequences for cellular functions, including CVR itself (Model et al., 2020) . Macromolecular crowding also induces liquid-liquid phase separation as part of the cellular osmosensing system (Ishihara et al., 2021; Watanabe et al., 2021) .

As mentioned above, in myoblasts, an acute decrease of PM tension induces macropinocytosis. This was established by hypotonic swelling (stretching the PM and inducing RVD) of the cells followed by returning to isosmotic conditions (inducing cell shrinkage and PM relaxation) (Loh et al., 2019) .

Both PIPs and IPs are involved in CVR. As shown in Figure 3 , PI(4,5)P2 is metabolized to Ins(1,4,5)P3 and further to the various inositol (poly)phosphates (Hatch and York, 2010) . Prominently, Ins(1,4,5)P3 binds to its receptor on internal Ca 2+ stores like the endoplasmic reticulum (ER) and triggers the release of Ca 2+ into the cytosol (Berridge, 2009) . Given the plentitude of cellular functions governed by Ca 2+ i and the numerous components of CVR dependent on it, Ins(1,4,5)P3 is crucial to it. Notably, the levels of IPs are altered in Rastransformed cells (Fleischman et al., 1986; Maly et al., 1995; Ritter et al., 1997a) . Besides, other inositol (poly)phosphates are activated or inhibited by anisotonicity and/or changes in CV (Fleischman et al., 1986; Lang et al., 1998; Jakab et al., 2002; Pesesse et al., 2004; Hoffmann et al., 2009; Wundenberg and Mayr, 2012; Lee et al., 2020) .

FIGURE 3 | Synopsis of various aspects in macropinocytosis and ion transport in cell volume regulation and vesicular volume regulation, which are also relevant in methuosis. Red dotted arrows indicate action/s on target/s; black arrows indicate metabolic conversion; green combs, phosphoinositides; red combs, inositolphosphates; yellow combs, sugars; green squares, important metabolites; orange letters, ion(s); blue letters, enzymes; ECF, extracellular fluid; ICF, intracellular fluid; IVF, intravesicular fluid; G, heterotrimeric G protein; TKR, tyrosine kinase receptor; NKA, Na + /K + -ATPase; SOCE, store-operated Ca 2+ entry; , transmembrane electrical potential difference. For the meaning of colored asterisks, see insert in the right lower part. For details, nomenclature, and further abbreviations, see text and list of abbreviations.

In programmed CD, cell shrinkage is characteristic (though not universal) of apoptosis and in its initial phase accomplished by RVD-like cellular exit of ions, termed apoptotic volume decrease (AVD). In contrast, cell swelling is characteristic of necrosis and ischemic cell death/onkosis (derived from the Greek word óγκoς, i.e., tumor/swelling Von Recklinghausen, 1910; Majno and Joris, 1995; Weerasinghe and Buja, 2012) , termed necrotic volume increase (NVI) (Okada et al., 2001; Orlov and Hamet, 2004; Lang and Hoffmann, 2012, 2013a,b; Orlov et al., 2013; Bortner and Cidlowski, 2014, 2020; Model, 2014; Okada, 2020) , and related modes of CD such as secondary necrosis, pyroptosis, or ferroptosis (Zong and Thompson, 2006; D'Arcy, 2019; Nirmala and Lopus, 2020; Riegman et al., 2020) . In methousis, not only is cell shrinkage absent but cells actually are swollen (Overmeyer et al., 2008) , such as seen in cells expressing an activated form of the H-Ras G12V oncoprotein (see below) (Maltese and Overmeyer, 2014; Alonso-Curbelo et al., 2015) . This may be related to altered ion transport in cells expressing the H-Ras oncogene.

The set point of a given cell for regulation of its volume is not a fixed constant but may change intrinsically to adjust the CV to altered functional needs. For instance, proliferating cells have to gain volume prior to mitosis, and hence, the set point for volume regulation varies in a cell cycle-dependent manner (Lang et al., 1992a Doroshenko et al., 2001; Chen et al., 2002; Klausen et al., 2007; Pedersen et al., 2013) . Expression of H-Ras G12V in NIH 3T3 fibroblasts leads to an upshift of the set point for CVR, i.e., the cells swell (Lang et al., 1992a) . This is related to alterations of cellular metabolism, ion transport, and structural remodeling. Such cells proliferate independently of growth factors and have altered PI metabolism (Fleischman et al., 1986; Maly et al., 1995) , stimulated Ca 2+ influx (Maly et al., 1995; Ritter et al., 1997b) , and increased intracellular pH (pH i ) due to activation of NHE (Maly et al., 1989; Ritter et al., 1997b) and Na + , K + , 2C1 − cotransport (NKCC1) (Lang et al., 1992a) . Furthermore, mitogens, like serum or bradykinin, cause pulsatile release of Ca 2+ from internal stores and activation of a store-operated calcium entry (SOCE), which leads to oscillations of PM via Ca 2+ -activated K + channels, NHE activation, and actin depolymerization (Lang et al., 1992b; Dartsch et al., 1994b Dartsch et al., , 1995 Ritter et al., 1997b) . In NIH 3T3 fibroblasts that do not express the H-Ras oncogene, bradykinin causes a single transient hyperpolarization, is without effect on actin stress fibers, and leads to cell shrinkage, unless actin is depolymerized (Lang et al., 1992b ) (for review, see Ritter and Woll, 1996) . The H-Ras-induced PM oscillations are stimulated by hypertonic cell shrinkage (Ritter et al., 1993) presumably via fostering physical apposition and hence interaction of STIM and Orai, which accomplish SOCE (Lang et al., 2018) . In line with that, SOCE has been shown to be inhibited by cell swelling (Liu et al., 2010) . Furthermore, SOCE is stimulated by hypertonicity via NFAT5 (TonEBP) (Sahu et al., 2017) and SGK1 (Lang et al., 2018) . Oncogenic K-Ras G13D has been shown to suppress SOCE by altered expression of STIM1 in colorectal cancer cells (Huang and Rane, 1993; Pierro et al., 2018) . Interestingly, fibroblastic L cells also display spontaneous oscillations of the cell membrane potential driven by oscillations of Ca 2+ i and concomitant K + channel activation. These oscillations are modulated by lowand high-density lipoproteins in parallel with Ca 2+ -dependent stimulation of their pinocytosis (Tsuchiya et al., 1981; Rane, 1991; Huang and Rane, 1993) . Oncogenic H-Ras also activates volume-regulated anion channels (VRACs) (Schneider et al., 2008) . An inverse relationship between CV and cell number has been described for T24H-Ras (H-Ras G12V ) bearing Rat1 and M1 fibroblasts (Kunzschughart et al., 1995) .

The ionic composition of the extracellular fluid affects pinocytosis by modulating the surface charge of the cell membrane and the cell membrane potential. Electrostatic interactions with the [normally negative (Cevc, 1990) ] outer surface charge of a cell and/or the presence of positive charges in macromolecules play an important role in the induction of pinocytosis. In Amoeba proteus, monovalent cations induce pinocytosis in the order Cs + >K + >Na + >Li + , while divalent cations are less effective. Critically, Ca 2+ and Mn 2+ reduce the sensitivity of monovalent cations but are themselves without effects on pinocytosis (Stockem, 1966; Josefsson, 1968; Josefsson et al., 1975) . Furthermore, the initial binding of pinocytosis inducers to the outer cell surface promotes displacement of surface-associated Ca 2+ ions and induces changes in various membrane parameters, such as increased membrane conductance and decreased PM along with an increase in plasmalemma hydration. Although pinocytosis in the amoeba can be induced by a great number of different solutes, all of them characteristically possess net positive charges that interact with negative surface charges. Hence, increasing extracellular Na + displaces most of the exchangeable surface-associated Ca 2+ and, therefore, induces pinocytosis. In the yolk sac, cationic macromolecules lead to pinocytosis (Lloyd, 1990) . In human corneal epithelial cells, fibroblasts, and human umbilical vein endothelial cells (HUVECs), the uptake of an antibody-drug conjugate by macropinocytosis is facilitated by the presence of positive charges or hydrophobic residues on the surface of the macromolecule . In contrast, in mammalian macrophages, anionic molecules are better inducers of pinocytosis than neutral or cationic ones (Cohn and Parks, 1967) . The uptake of positively charged nanoparticles in colon carcinoma Caco-2 cells is reduced by inhibition of macropinocytosis with 5-(N-ethyl-N-isopropyl)-amiloride (EIPA) and by cholesterol depletion of the PM, whereas these inhibitors have no effect on negatively charged systems (Bannunah et al., 2014) . Modulation of the charge for intracellular delivery carriers aims at increasing the efficiency of macropinocytosis of cellular entry for therapeutic nucleic acids to tumor cells (Desai et al., 2019) .

Anisotonicity modulates endocytosis in a diversity of cell types. In rat Kupffer cells, hypoosmotic and hyperosmotic conditions have been shown to stimulate and inhibit, respectively, phagocytosis (Warskulat et al., 1996) , and in microglial BV-2 cells, preconditioning with hypotonic or hypertonic medium attenuates microsphere uptake (Harl et al., 2013) . Hypertonic medium has been shown to disrupt the interaction of caveolae with endosomes. Increased phosphorylation due to phosphatase inhibition induces removal of caveolae from the PM. In the presence of hypertonic medium, this is followed by their redistribution to the center of the cell close to the microtubuleorganizing center .

Only a few studies have addressed the action of anisotonicity on pinocytosis. In Dictyostelium, hyperosmotic conditions lead to a decrease in endocytic activity that can be attributed to cellular acidification (Pintsch et al., 2001) , and it can foster the formation of giant vacuoles of pinocytotic origin, which appear to have a function in cellular osmoregulation (see above) (Yuan and Chia, 2001) . In murine bone marrow-derived macrophages, hypotonic cell swelling stimulates phagocytosis and pinocytosis, both of which appear to require ClC-3 chloride channels. In this study, the ion channels have on the one hand been suggested to act as volume-activated anion channels in the PM and, on the other hand, shown to support acidification the endosomal compartment (Yan et al., 2014) . Hypertonicity inhibits pinocytosis in rat hepatocytes (Synnes et al., 1999) . In mouse L929 fibroblasts, cellular uptake of horseradish peroxidase is inhibited in hypertonic sucrose medium, which is reversed to stimulated uptake in the presence 10% PEG 1000 in the medium (Okada and Rechsteiner, 1982; Hughey et al., 2007) . Similarly, in murine embryonic fibroblasts, cellular uptake of native proteins via macropinocytosis in hypertonic conditions is stimulated by alkali metal ions (Na + , Rb + , K + , Li + ) but not by hypertonicity created by addition of sugars or sugar alcohols. This finding points to the contribution of surface charge in macropinocytosis. Also, hypotonic stress has been demonstrated to greatly enhance receptor-independent retroviral transduction efficiency in NIH 3T3 fibroblasts via stimulated intensive endocytosis (Lee and Peng, 2009 ).

An interesting concept explaining how extracellular pH elicits such effects is that protonation of the cell surface produces local charge asymmetries across the cell membrane, which induce inward bendings of the lipid bilayer, thus favoring vesicle formation and uptake of macromolecules (Ben-Dov and Korenstein, 2012) .

In dendritic cells and bone marrow-derived macrophages, extracellular acidosis improves uptake and presentation of antigens by stimulation of macropinocytosis (Vermeulen et al., 2004; Martínez et al., 2007) . This effect is related to acid-sensing ion channels (ASICs) and can be inhibited by their blocker amiloride (Kong et al., 2013) . As macropinocytosis depends on phospholipase C (Amyere et al., 2002; Yoshida et al., 2015) , its activation by proton-sensing G protein-coupled receptors such as ovarian cancer G protein-coupled receptor 1 (OGR1/GPR68), G protein-coupled receptor 4 (GPR4), T-cell death-associated gene 8 (TDAG8/GPR65), and GPR132/G2A (Seuwen et al., 2006; Alexander et al., 2017; Insel et al., 2020) may also explain the observed stimulatory effect of extracellular acidosis on pinocytosis. Notably, in HEK293 cells expressing mutated wild-type OGR1 or mutated OGR1 L74P , receptor internalization, Ca 2+ i mobilization, and morphological changes observed upon activation of OGR1 by H + or Ni 2+ are severely compromised. This missense mutation of the H + ion-sensing receptor has been found to cause familial amelogenesis imperfecta (Sato et al., 2020) . Experiments with HEK293 cells transfected with active OGR1 receptor or a mutant lacking five histidine residues (H5Phe-OGR1) unraveled that receptor activation by H + stimulates NHE-and v-ATPase activity only in OGR1-but not in H5Phe-OGR1-transfected cells. Furthermore, the known OGR1 inhibitors Zn 2+ and Cu 2+ reduce the stimulatory effect (Mohebbi et al., 2012) . Given the importance of NHE1 and the v-ATPase for pinocytosis, it is tempting to speculate that OGR1 is a regulator thereof.

By contrast, in pancreatic acinar cells, low extracellular pH selectively impairs apical endocytosis. This is seen in mice lacking cystic fibrosis transmembrane conductance regulator (CFTR), which normally couples endocytosis at the apical PM to HCO 3 − secretion into the ductal lumen by normally rendering it alkaline. The acidic luminal fluid and impaired endocytosis due to lack of CFTR can be restored by alkalizing it in vitro (Freedman et al., 2001) .

Reorganization of the submembranous cytoskeleton is an essential step in pinocytosis. The cortical actin network is a major determinant of cell stiffness, and a correlation between stiffness of the actin network and the activity of endocytosis has been demonstrated (Planade et al., 2019) . Extracellular K + and Na + antagonistically modulate the gel-tosol transition of the cortical actin cytoskeleton beneath the PM (for review, see Oberleithner et al., 2009; Callies et al., 2011; Oberleithner and De Wardener, 2011; Warnock et al., 2014) . Elevations of extracellular Na + and K + concentration stiffen and soften, respectively, the submembranous actin cytoskeleton of endothelial cells within minutes (Oberleithner et al., 2009 ). The Na + -dependent stiffening is mediated by an aldosterone-induced upregulation and activation of epithelial Na + channels (ENaCs) and, presumably, by downregulation of the endothelial nitric oxide synthase (eNOS) activity. Conversely, inhibition of ENaCs upregulates nitric oxide [NO; formerly termed endotheliumderived relaxing factor (EDRF)] production. Depolarizing PM by increasing extracellular K + concentration, by blocking K + channels with Ba 2+ , and by decreasing extracellular Cl − concentration decreases the mechanical stiffness of endothelial cells (Callies et al., 2011) . Thus, modulation of the sol-gel transition of the actin cytoskeleton is thought to be due to the G-actin-dependent activation of eNOS (Fels et al., 2010) .

It has to be mentioned that hypotonic swelling of endothelial cells was shown to cause stiffening of the PM due to an increase in cellular hydrostatic pressure rather than to disruption of the submembranous actin network. In this study, a change in membrane tension was not observed upon osmotic swelling, and depolymerization of F-actin did not abrogate swelling-induced stiffening of the PM (Ayee et al., 2018) .

In endothelial cells, a reciprocal regulatory relationship between eNOS and caveolin-1 (Cav-1) has been found, whereby Cav-1 and eNOS regulate the function of each other. On the one hand, Cav-1 stabilizes eNOS expression and regulates its activity, and eNOSderived NO promotes caveolae-mediated endocytosis of albumin and insulin; on the other hand, a sustained NO production and persistent S-nitrosylation of Cav-1 lead to its ubiquitination and degradation (Chen et al., 2018) .

Besides eNOS, also an association of inducible NO synthase (iNOS) with the submembranous actin cytoskeleton and intracytoplasmic vesicles in lipopolysaccharide (LPS)-and interferon gamma (INFγ)-stimulated macrophages and other cells was shown (Webb et al., 2001; Su et al., 2005) .

Nitric oxide is a regulator of endocytosis, phagocytosis, and vesicle trafficking (Weinberg, 1998; Chen et al., 2018) . For example, NO downregulates endocytosis in rat liver endothelial cells (Martinez et al., 1996) but promotes caveolae-mediated endocytosis as mentioned above (Chen et al., 2018) . Macrophages stimulated by the thymic peptide thyαl display enhanced NO levels as well as increased pinocytosis (Shrivastava et al., 2004) , as do amphotericin B-stimulated microglial cells (Kawabe et al., 2017) . Conversely, mouse peritoneal macrophages stimulated with activin A display both reduced NO and pinocytosis (Zhou et al., 2009) . Also, macropinocytosis of drugs may promote enhancement of iNOS expression, as exemplified for the chemotherapeutic nab-paclitaxel, which synergizes to this end with INFγ (Cullis et al., 2017) .

Recently, it has been shown that NO regulates endocytic vesicle budding by S-nitrosylation of dynamin-a GTPase that regulates vesicle budding from the PM-and increases its enzymatic activity in response to NO (Wang et al., 2006) . Dynamin is bound by NOS trafficking inducer (NOSTRIN), an eNOS-interacting adaptor protein, which forms a complex with Cav-1 and eNOS, and colocalizes with WASP and actin to promote its polymerization. It thus regulates caveolar endocytosis and eNOS internalization (Schilling et al., 2006; Su, 2014) .

Nitric oxide/EDRF is also released in response to changes in extracellular osmolarity, which also greatly affect the cortical actin organization. This is also relevant in apoptosis, where NO metabolism, actin organization, and CV-regulatory ion transport are linked together (Bortner, 2005) .

Nitric oxide release in response to hypertonicity occurs in vessels and endothelial cells (Steenbergen and Bohlen, 1993; Vacca et al., 1996; Zani and Bohlen, 2005) . Hypertonicity downregulates eNOS in human aortic endothelial cells (HAECs), an effect that is mediated by activation of AQP1 and NHE-1, and which involves PKCβ-mediated intracellular signaling (Madonna et al., 2010) . In insulin-treated HAECs, hypertonicity, established by high glucose or mannitol, downregulates the PI3K/Akt/mTOR/eNOS pathway and impairs their ability to respond to insulin. This may contribute to insulin resistance. The mechanism involves AQP1 and the transcription factor Ton/EBP (NFAT5) for osmosensing, and the effect can be reversed by silencing the transcription of these proteins (Madonna et al., 2020) .

Hypotonic swelling has been shown to promote the release of NO and reactive nitrogen oxide species in various cells (Kimura et al., 2000; Haussinger and Schliess, 2005; Takeda-Nakazawa et al., 2007; Kruczek et al., 2009; Gonano et al., 2014) and to augment LPS-triggered iNOS expression in RAW 264.7 macrophages (Warskulat et al., 1998) . Recently, it has been shown that in HUVECs, VRAC/SWELL1/LRRC8A mediates endothelial cell alignment via stretch-dependent Akt-eNOS signaling and formation of a signaling complex made up by Grb2, Cav-1, and eNOS (Alghanem et al., 2021) .

While osmotic shrinkage in general is associated with an increase in actin polymerization, cell swelling leads to its depolymerization, though both with exceptions (Hoffmann et al., 2009) .

Hypertonic shrinkage fosters the translocation of the actinbinding protein cortactin to the cortical actin net, where it interacts with the Arp2/3 complex, WASP, dynamin, and myosin light-chain kinase (for review, see Pedersen et al., 2001; Hoffmann et al., 2009) . Cortactin stabilizes microfilament assembly at the cell periphery, is recruited to PM ruffles, and participates in macropinocytosis (Mettlen et al., 2006) . In humaninduced pluripotent stem cells, hyperosmolarity, created by high glucose or mannitol, upregulates AQP1 and induces cytoskeletal remodeling with increased ratios of F-actin to G-actin, effects that could be by reversed siRNA-mediated inhibition of AQP1 expression (Madonna et al., 2014) .

Cell swelling can lead to reorganization of intermediate filaments (Dartsch et al., 1994a; . In Cos-7 fibroblast-like cells, hypotonicity causes a rapid calcium/calpaindependent cleavage of the intermediate filament vimentin, whereby hypotonicity leads to the generation of IP 3 by hydrolysis of PI(4,5)P2 and Ca 2+ release from the ER (Pan et al., 2019) . Vimentin in turn is involved in the regulation of vesicular trafficking (Margiotta and Bucci, 2016) and was also shown to be modulated by NO (Sripathi et al., 2012) .

A distinctive hallmark to distinguish clathrin-dependent endocytosis from clathrin-independent macropinocytosis is the sensitivity of the latter to inhibitors of NHEs such as amiloride, EIPA, or HOE-694 (West et al., 1989; Koivusalo et al., 2010; Canton, 2018; Lin et al., 2020) . Besides NHEs, amiloride also directly inhibits ion channels of the epithelial sodium channel/degenerin family, such as ENaC and ASIC1 (Kellenberger and Schild, 2002; Qadri et al., 2010) and growth factor receptor tyrosine kinase activity (Davis and Czech, 1985) . Moreover, amiloride is a weak permeant base that can accumulate in acidic vesicles, thus, eventually dissipating the H + -gradient driving cation exchange, and thereby inhibiting pinocytosis (Dubinsky and Frizzell, 1983; Bakker-Grunwald et al., 1986) .

Of note, EIPA does not inhibit a newly described form of CD, which is induced by fenretinide, which is similar to methuosis characterized by hyperstimulated macropinocytosis and massive vacuolization (Brack et al., 2020) .

Na + /H + exchangers, which are members of the solute carrier (SLC) 9 family, are electroneutral transporters that exchange Na + for H + across membranes (Pedersen and Counillon, 2019) . The NHE1 isoform is expressed almost ubiquitously and regulates cellular and organelle pH, motility, phagocytosis, proliferation, as well as cell survival and CD (Orlowski and Grinstein, 2011; Pedersen and Counillon, 2019) . Cell shrinkage is a strong stimulator of NHE1 activity and serves for RVI (see above) (Ritter et al., 2001; Baumgartner et al., 2004; Orlowski and Grinstein, 2011; Pedersen and Counillon, 2019) . NHE1 is activated by a drop in pH i , a broad diversity of PM receptors, such as tyrosine kinase receptors, G-protein-coupled receptors or integrin receptors, and it is regulated by second messengers, asymmetric membrane tensions, and phosphoinositides, such as PI(4,5)P2 (Orlowski and Grinstein, 2011; Pang et al., 2012; Pedersen and Counillon, 2019) . In proximal tubular cells, NHE1 is activated by PI(4,5)P2 and inhibited by PI(3,4,5)P3 (Abu Jawdeh et al., 2011; Pang et al., 2012) . NHE1-borne changes in pH i may also be confined to distinct subcellular domains or compartments. Such subcellular compartments are the lammelipodia formed during cell spreading and migration, pseudopodia in phagocytosis, ruffles and the cup wall formation in macropinocytosis. In all of these processes, NHE1 is also involved in cytoskeletal rearrangement (Schwab and Stock, 2014; Pedersen and Counillon, 2019) . NHE1 is connected to actin via ezrin/radixin/moesin (ERM) family of actin-binding proteins. Migrating cells undergo cell polarity-dependent subcellular volume changes. Repetitive cycles of protrusion at the leading edge (lamellipodium) are followed by retraction of the cell's rear, i.e., the trailing edge. Waves of local water movement across the PM locally increase and decrease CV during the protrusion and retraction of the lamellipodium, respectively. Hence, cell migration requires an alternating cycle of subcellular RVI at the leading edge and RVD at the trailing edge of the cell. Most of the ion transporters known to participate in CVR are also involved in the regulation of cell migration. As part of the RVI transporters, NHE1 is confined to the lamellipodium, where it also serves for local extracellular acidification (Lang et al., 1998; Schneider et al., 2000; Loitto et al., 2002 Loitto et al., , 2009 Jakab and Ritter, 2006; Hoffmann et al., 2009; Schwab et al., 2012; Stock et al., 2013) . This is also given in breast cancer cell invadopodia, where NHE1 is allosterically regulated by NaV1.5 Na + channels (Brisson et al., 2012 (Brisson et al., , 2013 . In T47D human breast cancer cells, NHE1 colocalizes with Akt and ERK in prolactin-induced ruffles (Pedraz-Cuesta et al., 2016) .

Although inhibition of macropinocytosis by NHE inhibitors is a fingerprint feature of macropinocytosis, the molecular mechanisms how NHEs contribute to macropinocytosis are barely known. Interestingly, activation of the small GTPases, Rac1/Cdc42, and consequently actin polymerization and, thus, ruffle formation are sensitive to submembranous pH (pH sm ). Submembranous acidification due to inhibition of NHE1 by amiloride prevents Rac1/Cdc42 activation and suppresses actin polymerization (Koivusalo et al., 2010) . Moreover, NHE1 inhibition favors the accumulation of cytosolic H + , which neutralizes the negative charges on the inner leaflet of the PM. Thus the interaction of the negatively charged headgroups of phosphoinositides and the polybasic motifs in Rac and Cdc42 as well as the SCAR/WAVE complex and WASP is hampered. This also explains the inhibitory effect of NHE1 blockers on macropinocytosis (Marques et al., 2017) . Therefore, amiloride might not directly inhibit macropinocytosis but cause submembranous accumulation of metabolically generated H + in thin lamellipodium-like membrane extensions by inhibition of H + efflux. Given the small volume of these structures, only a few H + will change pH sm and, consequently, the state of actin polymerization. However, in HeLa cells, oncogenic Rasinduced macropinocytosis does not require a decrease in pH sm (Ramirez et al., 2019) .

Notably, in mice bearing xenograft tumors derived from oncogenic K-Ras bearing MIA PaCa-2 cells, intratumoral macropinocytosis and tumor growth are suppressed by treatment of the animals with EIPA, which is, however, effective only in tumors with high but not low macropinocytic activity (Commisso et al., 2013; Recouvreux and Commisso, 2017) .

The ionophoric NHE monensin induces vesicular swelling and the formation of giant multivesicular bodies (MVBs) (Stein and Sussman, 1986; Savina et al., 2003) and release of exosomes, which can be inhibited by the NHE inhibitor dimethyl amiloride (DMA) (Chalmin et al., 2010; Pironti et al., 2015) . Moreover, deletion of the Saccharomyces cerevisiae NHE, Nhx1, disrupts the fusogenicity of the MVB in a manner dependent on pH and monovalent cation gradients (Karim and Brett, 2018) .

After pinching off, the nascent macropinosome has entrapped extracellular fluid that equals the extracellular fluid in the intimate vicinity of its formation site. Traveling along the endolysosomal pathway, its ionic composition is modified to render the vesicular volume, shape, and fluid suitable for processing its cargo, as shown in Table 1 (Scott and Gruenberg, 2011; Freeman and Grinstein, 2018; Chadwick et al., 2021b) .

The permeabilities of the PM and vesicular membranes for water play crucial roles in pinocytosis. The importance of PM AQPs for macropinocytosis is seen in dendritic cells where they are needed as essential elements of a CV control mechanism necessary to concentrate the macrosolutes and present antigens (De Baey and Lanzavecchia, 2000; Hara-Chikuma et al., 2011; Kitchen et al., 2015) .

Macropinosomes shrink along their cellular route by moving ions and osmotically obliged water out of its lumen into the cytosol (Freeman and Grinstein, 2018; Chadwick et al., 2021b) . Despite the dramatic decrease in pinosome volume, the water permeation pathway is not characterized yet. Studies on osmotic water permeability (Pf) indicate that Pf values higher than 0.01 cm/s indicate water flux through channels (Verkman et al., 1996) , Pf values in the range from 0.003 to 0.005 cm/s designate membranes lacking water channels, and Pf values between 0.0001 and 0.005 cm/s characterize pure phospholipid bilayers (Fettiplace and Haydon, 1980; Echevarria and Verkman, 1992; Olbrich et al., 2000) . These values could be used to evaluate the presence or absence of water channels in endosomes.

Clathrin-coated vesicles from bovine brain that lack water channels have a low Pf of ∼0.001 cm/s and retain this value after stripping off the coat. Vesicles prepared from bovine renal cortex and inner medulla revealed two populations: one containing water channels with high (0.02 cm/s) and one lacking them with low values compared to brain-derived vesicles . Vasopressin-induced endosomes of rat kidney papilla and toad bladder endocytic vesicles have Pf values of 0.03 and >0.1 cm/s, respectively, whereas isolated toad bladder granules have a Pf value as low as 0.0005 cm/s (Verkman and Masur, 1988; Shi and Verkman, 1989; Verkman, 1989) .

Only a few studies have investigated Pf of pinosomes and lysosomes. In J774 macrophages, the Pf of the PM is in the range of ∼0.004-0.009 cm/s (Fischbarg et al., 1989; Ye et al., 1989; Echevarria and Verkman, 1992) . The water permeability of the macropinosome membrane of J774.A1 cells increases non-linearly from ∼0.001 cm/s 3 min after formation to ∼0.005 cm/s after 25 min of formation (Chaurra-Arboleda, 2009 ). The lysosomal Pf of CHO-K1 cells is in the same range (Chaurra et al., 2011) . In J774.A1 cells, Pf shows pH dependency. It decreases from ∼0.007 to 0.0009 cm/s following lysosomal alkalinization with NH 4 Cl (pH lys 6.5-6.8) and to 0.0001 cm/s after inhibition of the v-ATPase (pH lys ∼7.0). These values are similar to those in early macropinosomes, which have a pH of ∼6.7 (Chaurra-Arboleda, 2009 ). Taken together, the low Pf values of J774 macrophages indicate the absence of AQPs in the endolysosomal compartment.

Despite these specific observations, the role of AQPs in the endolysosomal compartment is poorly investigated, while their importance in volume regulation of secretory vesicles is well documented (Cho and Jena, 2006; Sugiya et al., 2008; Jena, 2020) . In toad urinary bladder endosomes, AQP-TB has been shown to be present (Siner et al., 1996) , and incorporation of PMderived AQP2 into endosomes is well known for AVP-stimulated renal collecting duct principal cells Verkman, 2005) . In astrocytes, AQP4-laden vesicles may fuse with the PM upon cell swelling (Potokar et al., 2013; Vardjan et al., 2015) . AQP6 was shown to be present in intracellular vesicles of acid-secreting intercalated cells of the renal collecting duct where it colocalizes with the H + -ATPase and serves also as an anion channel (Yasui et al., 1999; King et al., 2004) . Lack of AQP11 causes defective endosomal pH regulation, as seen in mice devoid of AQP11. This is accompanied by the appearance of huge vacuoles in the renal proximal tubules and polycystic kidneys (Ishibashi et al., 2009 ).

An example for the function of organellar AQPs in VVR and CVR is evident in Trypanosoma cruzi, as briefly described below.

Alternatively to AQPs, water permeation through transporters and ion channels has been described for glucose transporters (Loike et al., 1993; Fischbarg and Vera, 1995) , NKCC1, KCC, SGLT1, and CFTR (Hamann et al., 2010; Zeuthen, 2010; Zeuthen and Macaulay, 2012; Huang et al., 2017) . Eventually, these transporters or ion channels could be used in pinosomes for water efflux as well.

By whatever way, as upon hypertonic cell shrinkage also vesicles rapidly shrink, it can be deduced that the intrinsic water permeability is sufficiently high to allow for timely vesicular volume changes during resolution of macropinosomes (Freeman and Grinstein, 2018) . Using fluorescence enhancement of Lucifer yellow dextran by deuterated water, Li et al. (2020) have recently demonstrated that lysosomes rapidly swell in response to a hypoosmotic challenge, indicating that there is substantial water influx into the lumen of lysosomes soon after water penetration across the PM, again indicating that the intrinsic water permeability of these organelles is high.

The pH of the ingested fluid of nascent pinosomes resembles that of the extracellular fluid, which is under physiological conditions (7.4). During trafficking, vesicular pH (pH ves ) gradually decreases to the value prevailing in lysosomes, i.e., 4.5-5.0. pH ves regulates enzyme activities and enables oxidation reactions, the release of internalized receptors from their ligands and their recycling back to the PM, movement and assembly of organellar surface coat proteins, vesicle maturation, as well as membrane fusion processes (Demaurex, 2002; Ohgaki et al., 2011) .

The acidification is primarily achieved by insertion of the v-ATPase. The v-ATPase is an evolutionarily highly conserved primary active H + transporter. As already outlined, it can be found both in the PM and in various intracellular organelles. Structurally, it comprises a multiprotein complex that forms two distinct domains, i.e., the pore-forming transmembrane V O domain and the cytosolic V 1 domain. The latter hydrolyzes ATP to drive H + movement. This creates the electrochemical H + gradient across the membrane, thereby driving secondary active transport processes, which act together for proper adjustment of pH ves and vesicular ion and osmolyte composition. In particular, parallel to H + pumping, ion channels and transporters move Cl − , H + , and other cations to establish an electrical shut aiming for electroneutrality of the net charge transfer. Otherwise, the acidification would be limited by the transmembrane potential ves , which is set up by the v-ATPase itself (Demaurex, 2002; Steinberg et al., 2010; Koivusalo et al., 2011; Xiong and Zhu, 2016; Freeman and Grinstein, 2018; Jentsch and Pusch, 2018; Sterea et al., 2018; Li P. et al., 2019; Freeman et al., 2020; Chadwick et al., 2021a) . pH ves is stabilized by the buffering capacity of the vesicle content (∼60 mM/pH at pH 4.5-5) (Weisz, 2003; Steinberg et al., 2010) .

In addition, the v-ATPase also serves as a protein interaction hub. For the proper sorting and targeting of the vesicles, small GTPases are recruited to their membranes in an acidificationdependent manner. The v-ATPase itself is able to associate with some of them and with other regulatory proteins. Thus, the v-ATPase seems not only to serve for creating the acidic pH ves but also to sense it and to transmit this information to its cytoplasmic domain, thus enabling trafficking molecules to bind and perform their targeting functions. The v-ATPase is also involved in signaling pathways for the regulation of macropinocytosis, as outlined throughout this review. Its dysfunction may play critical roles in various diseases including diabetes, cancer, neurodegeneration, osteopetrosis, skin disorders, or renal tubular acidosis and other pathologies. Furthermore, it is also essential for viral entry into cells (for reviews on v-ATPase, see Nishi and Forgac, 2002; Platt et al., 2012; Maxson and Grinstein, 2014; Rappaport et al., 2016; Kissing et al., 2018; Futai et al., 2019; Collins and Forgac, 2020; Song Q. et al., 2020; Vasanthakumar and Rubinstein, 2020; Chadwick et al., 2021a,b; Eaton et al., 2021) .

Within 1 min after internalization, the luminal Cl − concentration in endosomes/macropinosomes drops from ∼120-150 mM to ∼20 mM (Sonawane et al., 2002) . This decrease is insensitive to Cl − channel inhibition and can be attributed to Cl − expulsion by an interior negative Donnan potential (Ohshima and Ohki, 1985; Sonawane and Verkman, 2003; Hryciw et al., 2012) . During maturation, the vesicular Cl − concentration increases again up to ∼130 mM in lysosomes (Saha et al., 2015) . The Cl − accumulation of late endosomes can be suppressed by inhibition of the v-ATPase and restored by the K + ionophore valinomycin. Also, replacement of Cl − by gluconate and Cl − channel inhibition slow endosomal acidification. Thus, Cl − is an important counter ion accompanying endosomal acidification (Sonawane and Verkman, 2003) (for review, see Faundez and Hartzell, 2004; Stauber and Jentsch, 2013) . The accumulation of lysosomal Cl − appears to be important for the adjustment of the lysosomal volume and the activity of proteases. Reduced lysosomal Cl − concentrations may lead to lysosomal storage diseases, e.g., Gaucher's disease (OMIM entries 230800, 230900, 231000) or Nieman-Pick's disease (OMIN entries 257200, 607616, 257220, 607625) (Cigić and Pain, 1999; Platt et al., 2012 Platt et al., , 2018 Stauber and Jentsch, 2013; Rappaport et al., 2016; Chakraborty et al., 2017; Astaburuaga et al., 2019) .

The Cl − channels and transporters expressed in intracellular organelles include the ClC family members ClC-3 through 7, chloride intracellular channels (CLICs), CFTR, AQP6, transmembrane proteins (TMEM)16C-G/anoctamin (ANO) 3-7, bestrophin-1, Golgi pH regulator (GPHR) (reviewed in Stauber et al., 2012; Stauber and Jentsch, 2013) , Tweety homolog 1 (Ttyh1) proteins (Wiernasz et al., 2014) , LRRC8 TMEM206 (Osei-Owusu et al., 2021) .

The ClC-3 to 7 transporters are confined to distinct endolysosomal compartments with partially overlapping appearance (Jentsch and Pusch, 2018) . They are electrogenic outwardly rectifying 2Cl − /H + exchangers working in parallel with the v-ATPase. As per exchange cycle, two Cl − enter and one H + leaves the vesicle, three negative charges accumulate in the vesicle. To maintain electroneutrality, three H + are pumped in, one of which leaves the vesicle again via the 2Cl − /H + exchanger, thus leading to a net uptake of two H + (Guzman et al., 2013; Jentsch and Pusch, 2018) . This proposed mechanism allows a more efficient H + and Cl − accumulation, as it generates a more inside-negative ves than an ohmic Cl − conductance could do. Yet, this mechanism may be restricted to ClC-5 (Zifarelli, 2015) . However, ves is generally thought to be negative (i.e., positive in the lumen) due to the rheogenic v-ATPase. The reason for this discrepancy is still elusive (Weinert et al., 2010 ) (reviewed in Jentsch, 2007 Stauber et al., 2012; Stauber and Jentsch, 2013; Jentsch and Pusch, 2018) . Dysfunctional ClC exchangers may lead to a broad variety of symptoms and disorders including neurodegeneration and other neuropathies, proteinuria and kidney stones, osteopetrosis, albinism, and lysosomal storage diseases (Jentsch and Pusch, 2018; Nicoli et al., 2019; Schwappach, 2020; Bose et al., 2021) . Overexpression of ClC-3 or gain-of-function mutations of ClC-6 or ClC-7/Ostm1 leads to swelling of late endosomes and lysosomes (Li et al., 2002; Nicoli et al., 2019; Polovitskaya et al., 2020) . In B-cell non-Hodgkin lymphoma cells, the PIKFyfe inhibitor apilimod leads to CD by formation of giant vacuoles and disruption of endolysosomal function, an effect that requires functional ClC-7/Ostm1 transporters (Gayle et al., 2017) . In Hela and NIH3T3 cells, a short natural ClC3 splice variant (Clc3s) has been shown to lead to the formation of large vacuoles (Wu et al., 2016) , and in Chinese hamster ovary CHO-K1 or human hepatoma Huh-7 cells, the volume of such vesicles is governed by their Cl − concentration (Li et al., 2002) .

Endothelial cells lacking CLIC4 display defective endothelial cell tubulogenesis and impaired acidification of large intracellular vesicles, while lysosomes are unaffected (Ulmasov et al., 2009) .

Previous work showed that cell swelling leads to alkalinization of acidic cellular vesicles, regardless whether the cells are swollen by hypotonicity, isoosmotically with high-K + solutions, inhibition of K + channels, or concentrative uptake of solutes such as amino acids. At least in liver cells, swellinginduced alkalinization occurs rather in the pre-lysosomal than lysosomal compartments. The increased pH ves affects proteolysis, trafficking of cell membrane proteins, and antigen presentation (Busch et al., , 1996 (Busch et al., , 1997 Völkl et al., 1994) . The mechanisms leading to swelling-induced rise of pH ves are still elusive. Interestingly, overexpression of a naturally occurring C-terminally truncated splice variant of mouse bestrophin-3, Best3V2, leads to, besides swelling, alkalinization of lysosomes (Wu et al., 2016) . Manipulation of the intracellular Cl − concentration leads to alterations of lysosomal volume due to the high ClC-3-endowed Cl − permeability. Accordingly, a decrease in intracellular Cl − concentration during RVD following cell swelling could alter pH ves . In H-Ras oncogene-expressing fibroblasts, which have a higher CV (see above), the swellinginduced vesicular alkalinization is less pronounced compared to cells not expressing the oncogene. Hence, in H-Ras-expressing cells, any effect of the swelling-induced vesicle alkalinization on cell function may be altered (Busch et al., 1997) . Notably, murine and human fibroblasts expressing oncogenic K-Ras also display significant alkalinization of lysosomes (Jiang et al., 1990) .

In several cell types, the fusion of late endosomes to lysosomes is prevented by isotonic K + buffers as a consequence of an increased permeability of cells to K + and concomitant cell swelling. This inhibition is selective for late endosomes, since other endosome fusion events, such as homotypic fusion of early or late endosomes or fusion of recycling endosomes with the PM, are not affected. Cell swelling is regarded to be causative for this effect (Ward et al., 1990) . As cell swelling and lack of fusion of endosomes to lysosomes are also hallmarks of methuosis (see below and Figure 2) , it is tempting to speculate that cell swelling in methuosis might not only be a passive consequence of swollen vacuoles but also be a cause of it.

A link between CVR and vacuolar pH regulation is also established by the proteins MLC1 and GliaLCAM, which are defective in megalencephalic leukoencephalopathy with subcortical cysts. This rare congenital disease is characterized by macrocephaly, ataxia, seizures, degeneration of motor functions, and cognitive decline, morphologically by chronic white matter edema and subcortical cysts, and on the ultrastructural level by intra-myelinic vacuole formation and enlarged intracellular vacuoles (Van Der Knaap et al., 2012) . The protein MLC1 is involved in astrocytic CVR. It may be a volume-sensitive ion channel itself, but it is also part of a macromolecular complex composed of the Na + /K + -ATPase, Kir4.1 K + -channels, AQP4, syntrophin, and caveolin-1, as well as volume-sensitive TRPV4 cation channels, which mediate cellular Ca 2+ influx upon cell swelling. In addition, MLC1 also influences CLC-2 chloride channels as well as the volume-sensitive anion channel(s)/current(s) (VRACs) and therefore also RVD. It has been shown that knockdown of LRRC8 annihilates the potentiating effect of MLC1 on VRAC currents via modulation of the phosphorylation state of the channel subunit LRRC8C (Elorza-Vidal et al., 2018) . GlialCAM serves as an escort protein for MLC1 and CLC-2, and it is necessary for its proper activation by cell swelling. MLC1 is expressed in early and recycling endosomes, which they use to travel to the PM during hyposmotic stress (Capdevila-Nortes et al., 2013) . Importantly, MLC1 also interacts with the v-ATPase, and it is involved in regulating early endosomal pH (Lanciotti et al., 2012) . Defective MLC1 may result in impaired recycling and retention of TRPV4 channels in the cytoplasmic perinuclear area and thus disturbed swellinginduced cellular Ca 2+ influx (Lanciotti et al., 2012) such as seen in monocyte-derived macrophages from MLC patients (Ridder et al., 2011; Petrini et al., 2013; Brignone et al., 2014 Brignone et al., , 2015 Elorza-Vidal et al., 2018) . Furthermore, CLC-2 knockout mice exhibit myelin vacuolization, which is thought to arise from dysregulation of extracellular ion concentrations (Stauber et al., 2012) . Moreover, ClC-2 channels are functionally regulated by SGKs by inhibiting the ubiquitin ligase Nedd4-2, which in turn results in reduced clearance of ClC-2 protein from the PM (Palmada et al., 2004) . As outlined above, SGK1 is involved in the regulation of macropinocytosis, and hence, both ClC-2 and SGKs may be tight together in the regulation of macropinocytosis.

The acid-activated outwardly rectifying chloride channel/current (ASOR; also termed proton-activated, outwardly rectifying anion current, PAC, PAORAC; or I Cl,H ) could be a candidate to serve as electrical shut for endolysosomal acidification. This anion channel or its core component is made up by TMEM206 proteins, which form a trimeric channel that is architecturally related to ENaCs/degenerin channels and ASICs (Ullrich et al., 2019; Ruan et al., 2020; Deng et al., 2021) . TMEM206 has been shown to interact with Akt and to contribute to acid-induced CD . The ASOR current is characterized by activation at pH values < 5.0, strong outward rectification, activation at positive transmembrane potentials, and sensitivity to typical Cl − channel blockers (Sato-Numata et al., 2013 , 2017 Kittl et al., 2019 Kittl et al., , 2020 Ullrich et al., 2019) . In contrast to CLCs, which are inhibited by extracellular/luminal acidic pH (Scheel et al., 2005; Jentsch, 2007; Jentsch and Pusch, 2018) , ASOR is activated by it. Accordingly, endosomal ASOR and CLCs could regulate ves and vesicular ion concentrations. TMEM206 is mainly localized in the PM, but variable cytoplasmic labeling has been documented (Ullrich et al., 2019) . Recently, it has been shown that TMEM206 indeed traffics from the PM to endosomes. Its deletion annihilates the endosomal Cl − conductance, raises the luminal Cl − concentration, lowers pH ves , and increases transferrin receptormediated endocytosis. Moreover, its overexpression generates a large endosomal Cl − current with properties resembling the endogenous conductance and reduces endosomal acidification as well as transferrin uptake. Thus, endosomal TMEM206 appears to function as a sensor for low pH and may prevent hyperacidification by releasing Cl − from the lumen (Osei-Owusu et al., 2021) . Clearly, the role of TMEM206/ASOR/PAORAC in the regulation for ves and pH ves warrants further investigations. Likewise, its role in VVR needs to be investigated.

LRRC8A-E/SWELL1 proteins are essential components of the volume-regulated outwardly rectifying Cl − current (VRAC, VSOR, VSOAC, ICl swell , ICl vol ) elicited in most cells during cell swelling (Qiu et al., 2014; Voss et al., 2014; Jentsch, 2016; Jentsch et al., 2016; Okada, 2020; Bertelli et al., 2021) , and they are involved in a broad variety of cellular functions . LRRC8A-E/SWELL1 also regulate the PI3K-Akt, Erk1/2, mTOR signaling cascade (Kumar et al., 2020; Alghanem et al., 2021) . Recently, Li et al. (2020) demonstrated the expression of LRRC8 family members on lysosomal membranes and their ability to build functional lysosomal VRACs (Lyso-VRACs). Patch clamp investigation of these vacuoles revealed that Lyso-VRACs are activated by ionic strength and permeable for anions, like Cl − , NO 3 − , HCO 3 − , acetate, aspartate, or glutamate. They are inhibited by known VRAC blockers. Early endosome membranes lack Lyso-VRACs. Hypoosmotic swelling promotes the formation of large cytoplasmic vacuoles containing markers for late endosomes and lysosomes with a diameter of >2 µm, which require functional Lyso-VRACs. Loss of VRAC channels enhances necrotic CD triggered by sustained hypoosmotic, hypoxic, and hypothermic stress. Furthermore, the authors demonstrated that endolysosome-derived giant vacuoles are subject to exocytosis. This releases PM tension and simultaneously reduces CV. Thus, by acting as water store-andrelease compartments, the swelling-induced giant vesicles have been compared to the "cell's 'bladder, ' sequestering intracellular excess water through vacuolation and then expelling the potentially toxic level of water through exocytosis, which also relieves the plasma membrane tension stress" .

AQP6 is activated by low pH and acts as Cl − channel in acid-secreting α-intercalated cells in renal collecting ducts. There it is found in endosomes where it colocalizes along with ClC-5 and the v-ATPase. However, ClC-5 is inhibited at acidic pH, whereas the anion conductance of AQP6 is turned on. Hence, ClC-5 may rather operate when acidification of the vesicles starts, whereas AQP6 may take over this role as their pH drops. Furthermore, AQP6, though its water permeability is low, may mediate vesicle swelling and membrane fusion during exocytosis or other cellular processes (Yasui et al., 1999; Hazama et al., 2002; Kitchen et al., 2015) .

The role of HCO 3 − in pinocytosis has not met much attention yet. Carbonic anhydrase (CA) catalyzes the dissociation of HCO 3 − to generate H + of CO 2 . CA isoforms are present at the PM, in the cytosol, and in lysosomes (Rikihisa, 1985; Reibring et al., 2014) . There they associate with numerous acid-base transporters such as anion exchangers 1-3 (AE1-3), sodium bicarbonate cotransporters NBCe1 and NBCn1, and NHE1 . Recently, Sedlyarov et al. (2018) found that electroneutral NBCn1 (NBC3, SLC4A7) is essential for phagosome acidification in macrophages. NBCn1 resides in the PM, and lack of it leads to cytoplasmic acidification, which in turn hampers phagosome maturation and impairs bacterial killing. If membrane-associated CA is internalized during the formation of the pinosomes, it will catalyze the dissociation of HCO 3 − to generate CO 2 , which in turn will rapidly equilibrate across the vesicular membrane. Notably, in global CVR, VRAC may actually work more effectively in driving RVD by passing HCO 3 − than Cl − , as the former can be virtually unlimitedly replenished from CO 2 in the presence of CA, whereas Cl − can only exit the cell as long as the PM is more negative than the equilibrium potential for this ion (Völkl and Lang, 1988; Ritter et al., 1991) . In line with these considerations, the VRAC channels made up of LRRC8A with B through E subunits are permeable to HCO 3 − (Gaitan-Penas et al., 2016). Hence, Lyso-VRAC may provide a vesicular HCO 3 − -conductive pathway as well.

Among the internalized ion transporters, the Na + /K + -ATPase may limit endosomal acidification. Its rheogenic Na + transport contributes to the vesicle of endosomes, thereby opposing H + transport. This is, however, only evident in early but not in late endosomes (Fuchs et al., 1989) . In line with this, it has been shown in Swiss 3T3 fibroblasts that inhibition of the Na + /K + -ATPase by ouabain strongly enhances the acidification of early but not late endosomes or lysosomes. Ouabain has also been shown to produce stronger endosomal acidification and parallel Cl − accumulation in transferrin-labeled early and recycling endosomes of J774 cells (Sonawane and Verkman, 2003) . Ouabain exerts its effect by acting on the interior side of the vesicles rather than on the PM (Zen et al., 1992) . In contrast, in early rat liver endosomes, the Na + /K + -ATPase does not regulate acidification (Anbari et al., 1994) . Whether internalized PM retrieved NHE1 contributes to pinosomal acidification remains to be determined. According to the prevailing Na + gradient between the lumen and the cytosol, NHEs should serve to acidify nascent macropinosomes. NHE1 does, however, not play a direct role in phagosome acidification due to the rapidly dissipating Na + gradient between the phagosome and the cytosol and the absence of the Na + /K + -ATPase to maintain such a gradient. Instead, phagosomal acidification is established by the v-ATPase (Hackam et al., 1997 (Hackam et al., , 1999 . By analogy, this also may hold true for macropinosomes.

The human orthologs of yeast NHX1 are the endosomal Na + (K + )/H + exchangers NHE-6 and NHE-9. NHE-6 is present in early and NHE-9 in late recycling vesicles. NHE-6 is also inserted into the PM upon vesicular recycling. NHE-6 and NHE-9 bind to the adaptor protein for activated PKC, receptor for activated C kinase (RACK1), which interacts with metabolic enzymes, kinase receptors, and ion transporters and contributes to the maintenance of pH ves by regulating the distribution of the transporters between endosomes and the PM. They are involved in clathrin-dependent endocytosis by alkalinizing early endocytic vesicles. Endosomes of NHE-6-deficient neurons appear to be strongly acidic. Such a hyperacidification occurs upon hypoxia-induced mobilization of NHE-6 to the PM (Lucien et al., 2017) . In mouse, loss of NHE-6 causes endolysosomal storage disease with accumulation of gangliosides and unesterified cholesterol in late endosomes and lysosomes of neurons of selective brain regions. In humans, mutations of NHE-6, NHE-7, and NHE-9 cause neurological syndromes (Ilie et al., 2014 (Ilie et al., , 2016 Kondapalli et al., 2014; Schwede et al., 2014) . NHE-5 may contribute to organellar pH regulation and regulate cell surface expression of the receptor tyrosine kinase MET and the EGF receptor (Fan et al., 2016) . NHE-7 is found in the trans-Golgi network and in endosomes and also interacts with RACK1. Interestingly, NHE-7 mediates an acidification of intracellular vesicles that adds to that set up by the v-ATPase and that accelerates endocytosis (Milosavljevic et al., 2014) . NHE-8 is found in the mid-to trans-Golgi compartment and MVBs. It regulates late endosomal morphology and function. In HeLa-M cells, NHE-8 silencing results in perturbation of MVB protein sorting, disrupted endosomal protein trafficking, and perinuclear clustering of endosomes and lysosomes (Lawrence et al., 2010) . In the kidney, it also localizes to the apical PM and regions of coated pits. NHA-2 appears to localize to multiple compartments. It is expressed in endosomes of pancreatic β cells and synaptic-like microvesicles and participates in clathrin-dependent but not -independent endocytosis (Fuster and Alexander, 2014) . In lysosomes, NHEs are absent (Nakamura et al., 2005) (for review, see Donowitz et al., 2013; Pedersen and Counillon, 2019) .

Na + /H + exchangers could fulfill a dual role in pH ves regulation. In endosomes, NHEs face the acidic organellar interior and the high K + concentration of the cytosol and act as H + /K + exchangers to alkalinize vesicles. Once recycled through the PM, they face the high Na + concentration within the nascent vesicle. As Na + is then following its gradient into the cytosol, H + is transported into the vesicular lumen, thus acidifying it (Pedersen and Counillon, 2019).

As mentioned above, extracellular and intracellular K + greatly affects endocytosis and vesicle trafficking. After pinching off, the pinosomes may retain the channel equipment of the PM, and they may contribute to the initial changes in the ionic composition of the intra-pinosomal fluid. Given the normally high K + conductance of the PM and the prevailing electrochemical driving force for K + , this is expected to be in favor of setting up a negative ves in the nascent pinosome, which in turn would initially drive its early ionic movements.

Following endosome formation, the intraluminal K + concentration changes from ∼5 mM to values in lysosomes ranging from 2 to 60 mM in a manner depending on pH lysosome (Steinberg et al., 2010; Sterea et al., 2018) . In maturing endosomes, the accumulation of K + has been shown to be dependent on cholesterol, as its depletion impairs this process (Charlton et al., 2019) .

The K + channel KCa3.1 is activated via PI(3)P and a putative regulatory subunit that is required for Ca 2+ gating. In addition, KCa3.1 interacts with the phosphatase myotubularin R6 (MTMR6), which dephosphorylates PI(3)P, thereby inactivating the ion channel (Srivastava et al., 2005 (Srivastava et al., , 2006 . Using coelomocytes of Caenorhabditis elegans, Maekawa et al. (2014) showed that the sequential dephosphorylation of phosphoinositides [in the order PI(3,4,5)P3, PI(3,4)P2, PI(3)P, PI] by phosphoinositide phosphatases, which are activated after ruffle formation, is related to the activation of KCa3.1. Before induction of macropinocytosis with EGF, KCa3.1 is enriched in "intracellular punctate structures" but not in the PM. Following EGF treatment, KCa3.1 is recruited to F-actin-positive membrane ruffles. Interestingly, exposure of coelomocytes to TRAM-34, a selective inhibitor of KCa3.1 (Panyi et al., 2006) , or using a mutant KCa3.1, which is not activated by PI(3)P, impairs cellular dextran uptake. TRAM-34 treatment does not inhibit ruffle formation or actin polymerization. Interestingly, supplementation by sucrose to cells treated with TRAM-34 restores macropinocytosis, indicating that KCa3.1 contributes to macropinocytosis at least partially by regulating local osmolarity via a decrease of the intracellular K + concentration. The authors suggest that KCa3.1 is involved in the closure of ruffles and that amiloride and TRAM-34 might be used to dissect macropinosome formation pharmacologicallyamiloride inhibits the formation and TRAM-34 the closure of the ruffle .

Endosomal and lysosomal K + concentration and vesicle are also regulated by the selective K + channel TMEM175, which in turn regulates organellar functions including fusions with each other. Via this channel, vesicular K + concentration and vesicle are hence also influenced by the cytosolic K + concentration. The "resting" lysosome is, however, also largely determined by the vesicular H + permeability. It is predicted to be in the range of −18 mV at pH 5.0 and +12 mV at pH 4.5 when assuming a cytosolic K + concentration of 140 mM (Cang et al., 2014 (Cang et al., , 2015 .

Furthermore, large-conductance Ca 2+ -activated K + channels (BK channels) are found in lysosomes. They are physically and functionally coupled to TRPML1 channels and facilitate release of Ca 2+ ions, which activate further BK channels. This hyperpolarizes lysosome and thus further facilitates Ca 2+ release. BK overexpression has been shown to rescue abnormal lysosomal storage in cells from patients with the lysosomal storage disease Niemann-Pick's disease (Cao et al., 2015) .

Ca 2+ ions are indispensable regulators of pinocytosis and endolysosomal functions. They induce macropinocytosis via F-actin depolymerization (Kabayama et al., 2009 ) and establish compensating exocytosis of large endosomes in parallel to ongoing macropinocytosis, thereby preventing cellular volume overload (Falcone et al., 2006) . Furthermore, they regulate lysosomal fusion events and condensation of the luminal content (Xu and Ren, 2015) .

Intravesicular H + and Ca 2+ concentrations are interdependently tied together. In 3T3 Swiss fibroblasts, after uptake of 2 mM extracellular Ca 2+ , the endosomal concentration rapidly drops to ∼30 µM within 3 min and further on to ∼3 µM after 20 min. This is proportionally paralleled by a drop of pH vesicle from 7.4 to 7.0 and 5.7 at the same time points. The loss of vesicular Ca 2+ can be prevented if the acidification is inhibited by blocking the v-ATPase. By reducing the external Ca 2+ to 200 µM, the acidification is completely suppressed. Hence, the acidification can occur only when the initial Ca 2+ concentration in the endosomes is high (Gerasimenko et al., 1998; Petersen et al., 2020) .

Lysosomal Ca 2+ homeostasis involves a putative Ca 2+ /H + exchanger and Ca 2+ pumps. The relationship between luminal Ca 2+ and H + is suggested to be as follows: the lower the pH lysosome , the higher the Ca 2+ concentration (Morgan et al., 2011) . In macrophages, the lysosomal Ca 2+ concentration is ∼500 µM and dependent on extracellular and cytosolic Ca 2+ concentration as well as on pH lysosome . Alkalizing pH lumen reversibly decreases Ca 2+ by shifting it into the cytosol. However, in contrast to fibroblasts, alterations of extracellular or lysosomal Ca 2+ do not alter pH lysosome in macrophages (Christensen et al., 2002; Sterea et al., 2018) . This model has been challenged by the finding that lysosomal stores are rather refilled pH-independently with Ca 2+ via the ER in an Ins(1,4,5)P3 receptor-dependent process and via involvement of an unknown Ca 2+ transport mechanism to move Ca 2+ into the lysosome (Garrity et al., 2016) .

The Ca 2+ permeability of the endolysosomal membranes is set up by several cation channels. Among them are the transient receptor potential channel family members TPRML1, 2, and 3 (mucolipins), two-pore channels TPC1, 2, and 3, and voltagegated Ca 2+ channels (Cheng et al., 2010; Abe and Puertollano, 2011; Grimm et al., 2012; Xiong and Zhu, 2016; Clement et al., 2020; Freeman et al., 2020; Jin et al., 2020) .

TRP channel of mucolipin subfamilies mainly localize to lysosomes and endosomes but are also found in the PM. The channels are activated by PI(3,5)P2. TRPML1 is inhibited by PI(4,5)P2, which is abundantly present in the PM. Hence, if residing there, it is likely to be functionally suppressed Venkatachalam et al., 2015) . TRPML1 is encoded by the Mcoln1 gene and expressed in late endosomes and lysosomes. It is responsible for the enlarged vacuole formation and the lysosomal storage disease mucolipidosis IV if mutated to loss of function (OMIM entry #252650) (Lloyd-Evans et al., 2008; Grimm et al., 2012) . The channel is permeable to Ca 2+ , Na + , K + , Fe 2+ , and Mg 2+ , and activated by PI(3,5)P2, but inhibited by PI(4,5)P2 and sphingomyelin. TRPML1 acts as a lysosomal pH regulator as it senses the pH lysosome and initiates the release of H + . Being activated at low pH, it allows for Ca 2+ influx . Accordingly, lysosomal acidification is hindered when PI(3,5)P3 generation is suppressed by inhibition or lack of PIKfyve and rescued by overexpression of TRPML1 or raising lysosomal Ca 2+ . However, the vacuole formation observed in PIKfyve-deficient cells is not rescued by Ca 2+ or overexpressed TRPML1 (Cheng et al., 2010; Isobe et al., 2019) .

PIKfyve converts PI(3)P into PI(3,5)P2 in the endocytic pathway and the enzyme Fig4 dephosphorylates it back to educt. In yeast, hyperosmotic stress leads to the rapid transient synthesis of PI(3,5)P2 via activation of PI(3)P kinase (Dove et al., 1997; Duex et al., 2006) and the TRPML1 ortholog Yvc1. Lack of PI(3,5)P2 synthesis leads to severe swelling of the endolysosomal compartment due to concentrating K + up to ∼85 mM. This effect is relieved by inactivating mutations of the vacuolar monovalent cation/H + -antiporter Vnx1 or v-ATPase or by activating mutations of Yvc1. This identifies PI(3,5)P3 and the ion transporters regulated by it as crucial osmoregulators (Dove et al., 1997; Wilson et al., 2018) . In contrast to yeast, hyperosmotic stress decreases and hypoosmotic treatment enhances PI(3,5)P2 production in monkey Cos-7 cells (Dove et al., 1997) . PI(3,5)P2 regulates vacuole size in part via TRPML1 channels (Krishna et al., 2016) . Hypertonicity also activates PIKfyve and its upstream regulator hVac14 in differentiated 3T3-L1 adipocytes, but not in their undifferentiated precursor cells (Sbrissa and Shisheva, 2005) . In dendritic cells, TRPML1 releases lysosomal Ca 2+ upon bacterial sensing, which activates the actin-based motor protein myosin II for directional migration. In addition, it induces the activation of the transcription factor EB (TFEB), which translocates to the nucleus to maintain TRPML1 expression. This TRPML1-TFEB axis results from the downregulation of macropinocytosis after bacterial sensing (Bretou et al., 2017) . Moreover, TRPML1 is a lysosomal sensor of reactive oxygen species (ROS) , which are known, on the one hand, to be generated during cell swelling (Gorg et al., 2013) and, on the other hand, to activate VRACs (Lambert, 2003; Varela et al., 2004; Browe and Baumgarten, 2006; Lambert et al., 2015) . Such a link between ROS, endosomal function, and CVR may be mediated by ClC-3-dependent endosomal ROS production and isosmotic activation of VRAC (Matsuda et al., 2010) .

Activating H-Ras mutations-known to drive macropinocytosis-elevates TRPML1 expression. In H-Rasdriven cancer cells, the channel is needed to restore PM cholesterol, which gets internalized during endocytosis. Inhibition or lack of TRPML1 causes false localization of cholesterol from the PM to endolysosomes and loss of oncogenic H-Ras from the cell surface (Jung and Venkatachalam, 2019) . Hence, cells expressing oncogenic H-Ras are vulnerable to inhibition of TRPML1 (Jung and Venkatachalam, 2019; .

TRPML2 is an osmo/mechanosensitive endolysosomal channel conductive to Ca 2+ , Na + , K + , and Fe 2+ and also activated by PI(3,5)P2. It is involved in Arf6-regulated recycling pathway and in endolysosomal membrane trafficking by means of its sensitivity to hypotonicity. In late endosomes, TRPML2 is activated by free cytosolic ADP-ribose and eventually by nicotinic acid adenine dinucleotide phosphate (NAADP) (Dong et al., 2010; Viet et al., 2019) . Mutations in TRPML2 may induce CD due to Ca 2+ overload Sterea et al., 2018; Chen et al., 2020a) .

TRPML3 localizes to both early endosomes and endolysosomes, is Ca 2+ permeable, activated by PI(3,5)P2, and inhibited by PI(4,5)P2, Na + , as well as acidic pH (Dong et al., 2010) .

The two-pore channels (TPCs) are voltage-gated cation channels that function as homodimers. TPC1 localizes predominantly to the proximal endosomal system, while TPC2 is found mainly on late endosomes and lysosomes, where they act as Na + and Ca 2+ release channels. They are activated by PI(3,5)P2 (rather than NAADP) Cang et al., 2013; She et al., 2019; Jin et al., 2020) and play important roles for vesicular fusion and endosomal trafficking, autophagy, nutrient sensing, protein processing, and macropinocytic virus entry Bellono and Oancea, 2014; Sakurai et al., 2015; Xiong and Zhu, 2016; Castonguay et al., 2017) . Inhibition or loss of TPC channels critically reduces cellular entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (Ou et al., 2020) and Ebola virus (Sakurai et al., 2015; Petersen et al., 2020) . TPC1 activity is high at alkaline pH vesicle and open over a wide voltage range but low at acidic pH vesicle . Also, low intracellular ATP concentration increases TPC1 opening. This leads to Na + efflux and thus facilitates the v-ATPase and luminal acidification (Cang et al., 2014) . TPC2 channels are important in organelle fusion and pH regulation. They mediate the rapid decrease of vesicular Ca 2+ after uptake (Petersen et al., 2020) . TPC2 inhibition or loss suppresses virus uptake (Sakurai et al., 2015; Petersen et al., 2020) , increases the risk to develop non-alcoholic steatohepatitis (NASH) and fatty liver disease (NAFLD); Grimm et al., 2014, p. 707] . TPC2 is also involved in pigmentation (Bellono and Oancea, 2014; Lin et al., 2015) .

Lysosomes seem also to possess voltage-gated calcium channel, which might serve to release Ca 2+ upon depolarization of vesicle and which are required for lysosomal fusion with endosomes and autophagosomes (Tian et al., 2015) .

Macropinosome shrinkage or swelling is accomplished by osmotically driven exchange of water between the vesicle lumen and the cytosol (VVR) (Freeman and Grinstein, 2018; Chen et al., 2020b; King and Smythe, 2020; Chadwick et al., 2021b) , in adjunction with global CVR (see above). The direction of ion flux-efflux or influx-is determined by the transmembrane ion gradients, the levels of PM and vesicle , and the reversal potentials of the respective ions. The composition of the fluid in a nascent macropinosome initially equals the fluid in the intimate extracellular vicinity of its emergence and may be identical to the extracellular fluid. This creates a transmembrane gradient across the membrane of the macropinosome and the cytosol similar to that across the PM. Macropinosome shrinkage is therefore largely mediated by Na + flux across the vesicle membrane. Recent studies demonstrated that Na + efflux down its gradient from the pinosome to the cytosol is controlled by the non-selective TRPML cation channels and TPC Na + channels (Laplante et al., 2002; Freeman et al., 2020; Chadwick et al., 2021a,b; Saric and Freeman, 2021) .

In addition to Na + , also Cl − permeates through the macropinosomal membrane (Freeman and Grinstein, 2018) . As described above, vesicular LRRC8 channels/LysoVRACs may play a role for the flux of anions across the vesicular membrane, and their importance in VVR has been recognized Osei-Owusu et al., 2021) . A role for TMEM206/ASOR/PAORAC seems feasible, and an eventual functional coupling of vesicular VSOR and ASOR channels/currents, as described to occur in the PM (Nobles et al., 2004; Lambert and Oberwinkler, 2005; Kittl et al., 2019 Kittl et al., , 2020 , awaits future investigation.

The efflux of Na + and Cl − is coupled to cotransporters such as the electroneutral cation-Cl − cotransporters (SLC12A family members; carrying K + ), NHEs (SLC9A family members; carrying H + ), and anion exchangers (AE; SLC4A family members; carrying HCO 3 − ) (Freeman and Grinstein, 2018) . Moreover, HCO 3 − forms water and CO 2 , which exits the macropinosome as described above. The net result of these ion fluxes leads to osmotically driven vesicular water exit and shrinkage in parallel to H + ion accumulation, thus generating small acidic late endosomes, in which the to-be-digested substances are highly concentrated (for review, see Freeman and Grinstein, 2018; Chadwick et al., 2021b) .

Accordingly, macropinosome shrinkage is prevented if Na + or Cl − is replaced by a non-permeant cation or anion. Importantly, the osmotically driven vesicle shrinkage is necessary for the vesiculation, tubulation, and scission processes, as it confersby creating membrane wrinkles-the appropriate curvature necessary to recruit and stabilize the protein complexes required for proper sorting and recycling of the vesicles and their cargo, e.g., anchor sites for microtubule-associated motors, branched actin generation, or cargo recognition proteins. The latter are composed of the sorting nexins (SNXs), which contain a Binamphiphysin-Rvs (BAR) domain. BAR domains are able to electrostatically interact with phospholipids of appropriately bent concave membrane stretches. Also, proteins of the ESCRT complexes take advantage of the slack membrane to induce inwardly budding intraluminal vesicles (ILVs) and further on MVBs. Thus, the vesicular shrinkage creates organelles with high surface-to-volume ratios, relieves the hydrostatic tension of their membranes, and is hence critical for their resolution (for review, see Freeman and Grinstein, 2018; Chen et al., 2020b; Freeman, 2020, 2021; Chadwick et al., 2021a,b) .

As pointed out above, the role of and function of AQPs in VVR is still elusive. A clue about their usage may come from observations in T. cruzi epimastigotes. They respond to cell swelling with trafficking and fusion of acidocalcisomes, which contain osmolytes and the AQP, TcAQP1, to the so-called bladder of the contractile vacuole complex, thereby loading their content to it. This enables the bladder to take up and store excess cellular water, whereby it swells by the aid of TcAQP1. Subsequently, the water is expelled to the extracellular environment, thus driving RVD (reviewed in Docampo et al., 2011 Docampo et al., , 2013 . The basic mechanistic principle of this mechanismsoak up, store, and release excess intracellular water to/from intracellular organelles-is somehow recapitulated in Cos1 cells in response to a hypotonicity as described above . It will be interesting to find out whether this principle of linking CVR and VVR is a more general one and if it might function in other cells as well.

Regulated CD in response to perturbation of the extracellular or intracellular milieu is an essential hallmark of multicellular organisms and is mediated mainly by apoptotic and necrotic phenotypes (Galluzzi et al., 2018) . Methuosis is a non-apoptotic CD phenotype characterized by large and lucent vacuoles, which are limited by a single membrane as well as by cell swelling and, finally, rupture of the PM (Maltese and Overmeyer, 2014) . Currently, methuosis is seen as a dysfunctional pinocytosis. Although Lewis first described macropinocytosis already in 1937 (Lewis, 1937) , the fatal consequences for the cell by "drinking too much" have been described by Overmeyer et al. (2008) several decades later. While macropinocytosis is associated with vesicle shrinkage during processing of the vesicle content, methuosis reflects the abnormal growth of pinosomes. Despite that cell vacuolization is a common feature in many diseases, such as the lysosomal storage disease Gaucher's disease or in microglia in Alzheimer's disease, it is still uncertain whether methuosis plays a critical role in the etiology of these diseases (Kruth et al., 2005; Rappaport et al., 2016) . There is also evidence that methuosis may be an eventual CD modality outcome of cell senescence (Adjemian et al., 2020) .

H-Ras is required for macropinocytosis, and oncogenic mutants of a Ras allele are associated with methuosis. Chi et al. (1999) tested the assumption that the persistent activation of an oncogenic mutant of a Ras allele induces CD on those malignant tumors, which in general do not show an expression of Ras alleles. They found that transfection of oncogenic H-Ras (Ras G12V ) in human malignant glioma cells and human gastric cancer cell lines causes CD associated with intense vacuolization (Chi et al., 1999) . These authors concluded that H-Ras expression triggers a type-2 CD, meaning that, in this condition, autophagy leads to CD. Overmeyer et al. (2008) replicated, confirmed, and extended these findings, but they excluded a type-2 form of CD. As in the study of Chi et al. (1999) , they used a human glioblastoma cell line and induced the expression of Ras. Within a few days, Ras-expressing cells revealed lucent cytoplasmic vacuoles in phase-contrast microscopy, which did contain neither cell organelles nor cytoplasmic components when analyzed using electron microscopy. Furthermore, electron microscopy revealed that the vacuoles are limited by a single and not by a double membrane, as would be distinctive for autophagy. Fluid-phase traces, like Lucifer yellow or dextran-Alexa Fluor 488, accumulate in large vacuoles, whereas transferrin-Alexa Fluor 594 labels small endosomes, which contain-in contrast to pinosomestransferrin receptors. Furthermore, the small endosomes are decorated with clathrin, whereas large vacuoles do not contain a clathrin coat. In contrast to functional pinocytosis, large vacuoles are not acidic. Finally, the cells detach from the surface and swell, and the PM ruptures. Because of the similarities with pinocytosis, but the fatal consequences of fluid uptake, the authors suggest that this is a dysfunctional pinocytosis, which leads to a distinct form of CD. Accordingly, Overmeyer et al. (2008) named this form of CD methuosis, derived from the Greek word methuo, meaning "to drink to intoxication."

In normal macropinocytosis, nascent macropinosomes dynamically engage with cytoskeletal elements and either are tagged for recycling or finally fuse with lysosomes. With methuosis, however, vesicles fuse with each other to form large vacuoles, leading to the presumption that methuosis is a dysfunctional pinocytosis (Maltese and Overmeyer, 2014) . In molecular-biological terms, methuosis shows its relationship to macropinocytosis by its dependence on H-Ras. Despite having the late endosome markers, LAMP1 and Rab7, vacuoles are not acidic, as demonstrated by their failure to accumulate acridine orange and LysoTracker. Interestingly, inhibition of v-ATPase by bafilomycin A1 also inhibits vacuolization (Maltese and Overmeyer, 2014 ). An important aspect in macropinocytosis is shrinkage of the vesicles via osmoregulatory processes (see above). Ras and phosphoinositides are required for the activation of a variety of ion channels, including those involved in vesicle shrinkage and CVR (see above) (Ritter and Woll, 1996) . Assuming that formation of large vacuoles is not only due to fusion of pinosomes but also related to organelle swelling, an imbalance in the signaling cascade from Ras to ion channels could play a key role in methuosis. In line with the crucial role of NHE1 in macropinocytosis is the observation that methuosis induced by an ursolic acid-derived small molecule, compound 17, has been shown to be prevented by EIPA (Sun et al., 2017) . As outlined above, H-Ras G12V -expressing cells experience to shift of CVR toward higher volumes and also the intracellular vesicles have more alkaline pH. Inducers of methuosis and formation of extremely enlarged vacuoles are given in Table 2 . Figure 3 highlights major cellular aspects of the interrelation of (macro)pinocytosis, CVR, and VVR as discussed in this review.

Understanding the mechanisms of pinocytosis and how it exerts its cell protective effects is critical to appreciate its diversity of physiological functions. It is also necessary to understand its dysfunctions and how they might lead to disease as well as to unravel how influencing pinocytosis may translate to potential applications for medical and other use.

For instance, designing of compounds like proteolytically stable peptidomimetics that are taken up by macropinocytosis, but which are able to escape from endosomes, could be used for intracellular biomolecular targeting (Yoo et al., 2020) .

Likewise, development of versatile drug carriers with a high loading capacity, such as nanoparticles, which are optimized for specific binding to cell surface receptors, and which are favoring both macropinocytic uptake and intracellular release by degradation of their shells, may open new ways for, e.g., cancer therapy. This principle was proven for hyaluronic acid-modified polymeric biodegradable mesoporous silica nanoparticles (Palanikumar et al., 2018) .

Cancer cells frequently depend on autophagy to support their metabolic and energetic demands. However, inhibition of autophagy leads to compensatory stimulation of macropinocytosis to ensure cellular nutrient supply. This switch depends on the transcription factor NRF2, which is recruited to promoter regions of macropinocytosis-related genes. Recently, it has been shown that dual inhibition of autophagy and macropinocytosis is a successful strategy in treating mice with pancreatic ductal adenocarcinomas. Advancing this strategy to clinical applications may be promising for cancer therapy (Staff, 2021; Su et al., 2021) .

Cell death by methuosis should meet special attention. The formation of huge cellular vacuoles, which eventually leads to cell death, is a frequently observed phenomenon in numerous pathologies and known for decades (Cameron, 1952; Henics and Wheatley, 1999) . However, in many studies, the origin and mechanisms of the development of such cellular vacuoles were not or could not be determined. The term methuosis was coined in 2008 (Overmeyer et al., 2008) . Hence, it will be necessary to carefully scrutinize the available body of literature for work describing methuosis-like phenomena and to eventually reevaluate it for better understanding the underlying mechanisms of deadly vacuolization and related types of cell death (Chi et al., 1999) .

Recent studies indicate that volume regulation in cells and cell organelles is governed by the same biophysical principles and by the overlapping use of ion channels and transporters in distinct cellular compartments Freeman, 2020, 2021; Chadwick et al., 2021a,b) . That is, it starts with the asymmetric distribution of ions and materializes in the well-orchestrated uptake and processing of engulfed material in specialized vesicles (Chadwick et al., 2021a) .

However, the contribution of inorganic ions may go beyond volume regulation. Inflammation and tissue injury are associated with a local extracellular increase in Ca 2+ and enhancement of macropinocytosis in antigen-presenting cells (Canton, 2018) . Presumably, the extracellular Ca 2+ concentration is sensed by the calcium-sensing receptor (CaSR), which is expressed in myeloid cells (Canton, 2018) . Accordingly, change in the extracellular ion composition may be critical in antigen processing and presentation via major histocompatibility complexes (MHCs) as well as recognition of non-self-antigens or modified endogenous substances by pattern recognition receptors (PRRs) (Canton, 2018) . Consequently, failure in proper pinocytosis due to the formation of large vesicles could impair degradation of organic compounds or its delivery to PRRs or MHCs, which may have detrimental consequences in the immune response. The Jefferies et al., 2008; Sbrissa et al., 2012 Sbrissa et al., , 2018 Compton et al., 2016; Sano et al., 2016; Saveanu and Lotersztajn, 2016; Gayle et al., 2017; Ikonomov et al., 2018; Li Z. et al., 2019 Rare earth oxide nanoparticles HeLa cells Leads to mTOR deactivation and nucleus translocation of TFEB *Not yet termed methuosis; instead designated as "type 2 physiological cell death" (autophagic degeneration). For abbreviations and acronyms see list of abbreviations.

studies on CVR and VVR, pinocytotic activity, and delivery of compounds to MHCs or PRRs exemplify the power of ion gradients to understand physiology and pathophysiology of pinocytosis and methuosis, respectively. Despite Ca 2+ , other inorganic ions may have comparable dual effects on volume regulation and delivering of ligands to MHCs or PRRs. Thus, identification of ion channels and transporters required for establishing ion gradients could lead to a more detailed understanding of the complex interaction between distinct inorganic ions and cellular responses to tissue injuries or inflammation. Beyond that, targeting specific ion transport mechanisms or their regulators will help to combat cellular entry of pathogens such as viruses or bacteria. For example, inhibition of PIKfyve, TPCs, NHEs, or the v-ATPase could help prevent cellular infection by Ebola virus, SARS-CoV-2, and other viruses (Mercer and Helenius, 2009; Sakurai et al., 2015; Kang et al., 2020; Mercer et al., 2020; Petersen et al., 2020; Spix et al., 2020; Eaton et al., 2021) .

The number of compounds and materials that can induce or inhibit methuosis in various cell types and organisms is growing, and the fields of their use are just emerging. It will be necessary to unravel not only their specific modes of actions but also their harmful effects, as they might be used for a broad variety of applications, e.g., for eco-friendly biological pest control in agriculture to overcome multidrug resistance of parasites (Bogner et al., 2017; . Importantly, they may emerge as new treatment options for various diseases, e.g., cancer (Song S. et al., 2020; Xiao et al., 2021) , ALS, or dementia (Fomin et al., 2018; Fomin, 2019) .

HK conceptualized the manuscript. HK, MR, and NB wrote and finalized the manuscript. NB captured and arranged the images displayed in Figure 1 and the accompanying Supplementary  Video 1. MR prepared Tables 1, 2, and Figures 2, 3 . All authors contributed to the article and approved the submitted version.

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcell.2021.

Supplementary Video 1 | Pinocytosis occurring in a multinucleated giant cell from a rat non-parenchymal hepatic cell line putatively representing immortalized monocytes (Kupffer cells). For explanations, see Figure 1 and text.

Supplementary Video 2 | Close-up of Supplementary Video 1.

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PAC proton-activated chloride channel contributes to acid-induced cell death in primary rat cortical neurons

Proton-activated chloride channel PAC regulates endosomal acidification and transferrin receptor-mediated endocytosis

Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

Active ras triggers death in glioblastoma cells through hyperstimulation of macropinocytosis

A chalconerelated small molecule that induces methuosis, a novel form of non-apoptotic cell death, in glioblastoma cells

Hyaluronic acid-modified polymeric gatekeepers on biodegradable mesoporous silica nanoparticles for targeted cancer therapy

Metabolic functions of macropinocytosis

Serum and glucocorticoid inducible kinases functionally regulate ClC-2 channels

Hypotonic stress induces fast, reversible degradation of the vimentin cytoskeleton via intracellular calcium release

On the role of the difference in surface tensions involved in the allosteric regulation of NHE-1 induced by low to mild osmotic pressure, membrane tension and lipid asymmetry

K+ channel blockers: novel tools to inhibit T cell activation leading to specific immunosuppression

MicroRNAs-103/107 coordinately regulate macropinocytosis and autophagy

Regulated internalization of caveolae

Channels and volume changes in the life and death of the cell

The SLC9A-C mammalian Na(+)/H(+) exchanger family: molecules

The cytoskeleton and cell volume regulation

Cell volume regulation in epithelial physiology and cancer

Prolactin signaling stimulates invasion via Na(+)/H(+) exchanger NHE1 in T47D human breast cancer cells

Signaling by higher inositol polyphosphates. Synthesis of bisdiphosphoinositol tetrakisphosphate

Endocytic uptake of SARS-CoV-2: the critical roles of pH, Ca2+, and NAADP

Monocytes and macrophages as biomarkers for the diagnosis of megalencephalic leukoencephalopathy with subcortical cysts

Oncogenic KRAS suppresses store-operated Ca(2+) entry and ICRAC through ERK pathway-dependent remodelling of STIM expression in colorectal cancer cell lines

Cytosolic acidification as a signal mediating hyperosmotic stress responses in Dictyostelium discoideum

Circulating exosomes induced by cardiac pressure overload contain functional angiotensin II type 1 receptors

Association of Rap1a and Rap1b proteins with late endocytic/phagocytic compartments and Rap2a with the Golgi complex

Mechanical stiffness of reconstituted actin patches correlates tightly with endocytosis efficiency

The cell biology of disease: lysosomal storage disorders: the cellular impact of lysosomal dysfunction

Lysosomal storage diseases

Actin and actin-binding proteins

A recurrent gain-of-function mutation in CLCN6, encoding the ClC-6 Cl(-)/H(+)-exchanger, causes early-onset neurodegeneration

A unique platform for H-Ras signaling involving clathrin-independent endocytosis

Regulation of AQP4 surface expression via vesicle mobility in astrocytes

Amiloride docking to acid-sensing ion channel-1

SWELL1, a plasma membrane protein, is an essential component of volumeregulated anion channel

Macropinosome maturation and fusion with tubular lysosomes in macrophages

Nematicidal activity of 5-iodoindole against root-knot nematodes

Hydropic anthelmintics against parasitic nematodes

Nematicidal and insecticidal activities of halogenated indoles

Assessments of iodoindoles and abamectin as inducers of methuosis in pinewood nematode

Plasma membrane V-ATPase controls oncogenic RAS-induced macropinocytosis

A Ca2(+)-activated K+ current in ras-transformed fibroblasts is absent from nontransformed cells

A comparative study on the alterations of endocytic pathways in multiple lysosomal storage disorders

Macropinocytosis: a metabolic adaptation to nutrient stress in cancer

Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation

Mechanistic studies of anticancer aptamer AS1411 reveal a novel role for nucleolin in regulating Rac1 activation

Megalencephalic leucoencephalopathy with cysts: defect in chloride currents and cell volume regulation

Ferroptosis occurs through an osmotic mechanism and propagates independently of cell rupture

Cyclin-dependent kinase inhibitors exert distinct effects on patientderived 2D and 3D glioblastoma cell culture models

Ultrastructural localization of carbonic anhydrase in lysosomes

Effects of bradykinin on NIH 3T3 fibroblasts pretreated with lithium. Mimicking events of Ha-ras oncogene expression

Na(+)/H(+)exchangers: linking osmotic dysequilibrium to modified cell function

Activation of Na+/H(+)-exchanger by transforming Ha-ras requires stimulated cellular calcium influx and is associated with rearrangement of the actin cytoskeleton

Further characterization of volume regulatory decrease in cultured renal epitheloid (MDCK) cells. Pflügers Arch

Modification of cellular ion transport by the haras oncogene: steps towards malignant transformation

Cell shrinkage stimulates bradykinin-induced cell membrane potential oscillations in NIH 3T3 fibroblasts expressing the ras-oncogene

Dynamics of rab5 activation in endocytosis and phagocytosis

Synthesis and evaluation of indole-based chalcones as inducers of methuosis, a novel type of nonapoptotic cell death

Structures and pH-sensing mechanism of the proton-activated chloride channel

Sequential activities of phosphoinositide 3-kinase, PKB/Aakt, and Rab7 during macropinosome formation in Dictyostelium

Ouabain-induced cytoplasmic vesicles and their role in cell volume maintenance

Regulation of the actin cytoskeleton-plasma membrane interplay by phosphoinositides

A pHindependent DNA nanodevice for quantifying chloride transport in organelles of living cells

NFAT5-sensitive Orai1 expression and store-operated Ca(2+) entry in megakaryocytes

Two-pore channels control Ebola virus host cell entry and are drug targets for disease treatment

Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP

Vacuolin-1 inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition

Endomembrane tension and trafficking. Front

Solutes as controllers of endomembrane dynamics

PFKFB3 inhibition reprograms malignant pleural mesothelioma to nutrient stress-induced macropinocytosis and ER stress as independent binary adaptive responses

A missense mutation of Leu74Pro of OGR1 found in familial amelogenesis imperfecta actually causes the loss of the pH-sensing mechanism

Distinct contributions of LRRC8A and its paralogs to the VSOR anion channel from those of the ASOR anion channel

Acid-sensitive outwardly rectifying (ASOR) anion channels in human epithelial cells are highly sensitive to temperature and independent of ClC-3

Active vacuolar H+ ATPase and functional cycle of Rab5 are required for the vacuolation defect triggered by PtdIns(3,5)P2 loss under PIKfyve or Vps34 deficiency

Exosome release is regulated by a calcium-dependent mechanism in K562 cells

Functional dissociation between PIKfyve-synthesized PtdIns5P and PtdIns (3, 5) P2 by means of the PIKfyve inhibitor YM201636

Apilimod, a candidate anticancer therapeutic, arrests not only PtdIns(3,5)P2 but also PtdIns5P synthesis by PIKfyve and induces bafilomycin A1-reversible aberrant endomembrane dilation

Acquisition of unprecedented phosphatidylinositol 3,5-bisphosphate rise in hyperosmotically stressed 3T3-L1 adipocytes, mediated by ArPIKfyve-PIKfyve pathway

Voltage-dependent electrogenic chloride/proton exchange by endosomal CLC proteins

Translocation of endothelial nitric-oxide synthase involves a ternary complex with caveolin-1 and NOSTRIN

Macropinocytosis of the PDGF beta-receptor promotes fibroblast transformation by H-RasG12V

H-ras transformation sensitizes volume-activated anion channels and increases migratory activity of NIH3T3 fibroblasts

Volume dynamics in migrating epithelial cells measured with atomic force microscopy

Role of ion channels and transporters in cell migration

Ion channels and transporters in tumour cell migration and invasion

Chloride accumulation in endosomes and lysosomes: facts checked in mice

Genes for endosomal NHE6 and NHE9 are misregulated in autism brains

Ion flux and the function of endosomes and lysosomes: pH is just the start: the flux of ions across endosomal membranes influences endosome function not only through regulation of the luminal pH

The small Mr Ras-like GTPase Rap1 and the phospholipase C pathway act to regulate phagocytosis in Dictyostelium discoideum

The bicarbonate transporter SLC4A7 plays a key role in macrophage phagosome acidification

Receptors for protons or lipid messengers or both?

Structural mechanisms of phospholipid activation of the human TPC2 channel

Very high water permeability in vasopressininduced endocytic vesicles from toad urinary bladder

Activation of tumor-associated macrophages by thymosin alpha 1

Protease 3C of hepatitis A virus induces vacuolization of lysosomal/endosomal organelles and caspase-independent cell death

CK2 inhibition with silmitasertib promotes methuosis-like cell death associated to catastrophic massive vacuolization of colorectal cancer cells

Cloning of an aquaporin homologue present in water channel containing endosomes of toad urinary bladder

Chloride concentration in endosomes measured using a ratioable fluorescent Cl-indicator evidence for chloride accumulation during acidification

Determinants of [Cl-] in recycling and late endosomes and Golgi complex measured using fluorescent ligands

The emerging roles of vacuolartype ATPase-dependent Lysosomal acidification in neurodegenerative diseases

The dual role of macropinocytosis in cancers: promoting growth and inducing methuosis to participate in anticancer therapies as targets

TRPML cation channels in inflammation and immunity

Nitric oxide leads to cytoskeletal reorganization in the retinal pigment epithelium under oxidative stress

Combined mTORC1/mTORC2 inhibition blocks growth and induces catastrophic macropinocytosis in cancer cells

Phosphatidylinositol 3-phosphate indirectly activates KCa3.1 via 14 amino acids in the carboxy terminus of KCa3.1

The phosphatidylinositol 3-phosphate phosphatase myotubularin-related protein 6 (MTMR6) is a negative regulator of the Ca2+-activated K+ channel KCa3.1

Autophagy-deficient pancreatic cancer cells depend on macropinocytosis. Cancer Discov

Chloride in vesicular trafficking and function

Cell biology and physiology of CLC chloride channels and transporters

Sodium hyperosmolarity of intestinal lymph causes arteriolar vasodilation in part mediated by EDRF

Demonstration of two distinct transferrin receptor recycling pathways and transferrin-independent receptor internalization in K562 cells

A cation counterflux supports lysosomal acidification

Membrane flow during pinocytosis. A stereologic analysis

The hidden potential of lysosomal ion channels: a new era of oncogenes

Roles of ion transport in control of cell motility

Pinocytose und bewegung von amöben

Macropinocytosis: insights from immunology and cancer

Cancer cells escape autophagy inhibition via NRF2-induced macropinocytosis

Regulation of endothelial nitric oxide synthase activity by protein-protein interaction

Cytoskeletal regulation of nitric oxide synthase

Structural impact of hydrodynamic injection on mouse liver

Role of aquaporins and regulation of secretory vesicle volume in cell secretion

PIP2 is a necessary cofactor for ion channel function: how and why?

Novel role of TRPML2 in the regulation of the innate immune response

An ursolic acid derived small molecule triggers cancer cell death through hyperstimulation of macropinocytosis

Phorbol esters stimulate macropinocytosis and solute flow through macrophages

Shaping cups into phagosomes and macropinosomes

The breadth of macropinocytosis research

Macropinosomes as units of signal transduction

Fluid phase endocytosis and galactosyl receptor-mediated endocytosis employ different early endosomes

Hyposmotic stimulation-induced nitric oxide production in outer hair cells of the guinea pig cochlea

Rasactivated endocytosis is mediated by the Rab5 guanine nucleotide exchange activity of RIN1

Wnt canonical pathway activates macropinocytosis and lysosomal degradation of extracellular proteins

A voltage-gated calcium channel regulates lysosomal fusion with endosomes and autophagosomes and is required for neuronal homeostasis

FcRn mediates fast recycling of endocytosed albumin and IgG from early macropinosomes in primary macrophages

Differential induction of cytoplasmic vacuolization and methuosis by novel 2-indolyl-substituted pyridinylpropenones

Synthesis and biological evaluation of isomeric methoxy substitutions on anti-cancer indolyl-pyridinyl-propenones: effects on potency and mode of activity

Synthesis and biological evaluation of indolylpyridinyl-propenones having either methuosis or microtubule disruption activity

Lysosomal biology and function: modern view of cellular debris bin

Membrane potential changes associated with pinocytosis of serum lipoproteins in L cells

Identification of TMEM206 proteins as pore of PAORAC/ASOR acid-sensitive chloride channels

Chloride intracellular channel protein-4 functions in angiogenesis by supporting acidification of vacuoles along the intracellular tubulogenic pathway

Evidence that synthetic lethality underlies the mutual exclusivity of oncogenic KRAS and EGFR mutations in lung adenocarcinoma

The effects of hypertonic saline solution on coronary blood flow in anaesthetized pigs

Megalencephalic leukoencephalopathy with subcortical cysts: chronic white matter oedema due to a defect in brain ion and water homoeostasis

Osmotic swelling-induced activation of the extracellularsignal-regulated protein kinases Erk-1 and Erk-2 in intestine 407 cells involves the Ras/Raf-signalling pathway

Pathologic potential of astrocytic vesicle traffic: new targets to treat neurologic diseases?

NAD (P) H oxidase-derived H2O2 signals chloride channel activation in cell volume regulation and cell proliferation

Structure and Roles of V-type ATPases

A plasma membrane template for macropinocytic cups

The role of TRPMLs in endolysosomal trafficking and function

Functional water channels are present in clathrin-coated vesicles from bovine kidney but not from brain

Mechanisms and regulation of water permeability in renal epithelia

More than just water channels: unexpected cellular roles of aquaporins

Endosomes from kidney collecting tubule cells contain the vasopressin-sensitive water channel

Very low osmotic water permeability and membrane fluidity in isolated toad bladder granules

Water transport across mammalian cell membranes

Acidosis improves uptake of antigens and MHC class I-restricted presentation by dendritic cells

Structure of the human TRPML2 ion channel extracytosolic/lumenal domain

Alkalinization of acidic cellular compartments following cell swelling

Ionic requirement for regulatory cell volume decrease in renal straight proximal tubules

Inhibition of macropinocytosis blocks antigen presentation of type II collagen in vitro and in vivo in HLA-DR1 transgenic mice

Untersuchungen Über Rachitis und Osteomalacie

Identification of LRRC8 heteromers as an essential component of the volumeregulated anion channel VRAC

Cloning and characterization of a putative human serine/threonine protein kinase transcriptionally modified during anisotonic and isotonic alterations of cell volume

h-sgk serinethreonine protein kinase as transcriptional target of p38/MAP kinase pathway in HepG2 human hepatoma cells

Identification of a Ras GTPase-activating protein regulated by receptor-mediated Ca2+ oscillations

Nitric oxide regulates endocytosis by S-nitrosylation of dynamin

TPC proteins are phosphoinositide-activated sodium-selective ion channels in endosomes and lysosomes

Inhibition of late endosome-lysosome fusion: studies on the mechanism by which isotonic-K+ buffers alter intracellular ligand movement

Blood pressure and amiloride-sensitive sodium channels in vascular and renal cells

Compatible organic osmolytes and osmotic modulation of inducible nitric oxide synthetase in RAW 264.7 mouse macrophages

Modulation of phagocytosis by anisoosmolarity and betaine in rat liver macrophages (Kupffer cells) and RAW 264.7 mouse macrophages

Cells recognize osmotic stress through liquid-liquid phase separation lubricated with poly(ADP-ribose)

Macrophage nitric oxide synthase associates with cortical actin but is not recruited to phagosomes

Oncosis: an important non-apoptotic mode of cell death

Nitric oxide production and nitric oxide synthase type 2 expression by human mononuclear phagocytes: a review

Lysosomal pathology and osteopetrosis upon loss of H+-driven lysosomal Cl-accumulation

Acidification and protein traffic

A growth factor signaling cascade confined to circular ruffles in macrophages

N-cadherin regulates cell migration through a Rab5-dependent temporal control of macropinocytosis

Distinct endocytotic pathways in epidermal growth factor-stimulated human carcinoma A431 cells

Ttyh1 protein is expressed in glia in vitro and shows elevated expression in activated astrocytes following status epilepticus

Akt and SGK protein kinases are required for efficient feeding by macropinocytosis

Arf6, JIP3, and dynein shape and mediate macropinocytosis

PI(3,5)P2 controls vacuole potassium transport to support cellular osmoregulation

Biology of human sodium glucose transporters

A C-terminally truncated mouse Best3 splice variant targets and alters the ion balance in lysosome-endosome hybrids and the endoplasmic reticulum

Synthesis and biological actions of diphosphoinositol phosphates (inositol pyrophosphates), regulators of cell homeostasis

Macropinocytosis: mechanism and targeted therapy in cancers

Regulation of lysosomal ion homeostasis by channels and transporters

Lysosomal physiology

Increase in hypotonic stress-induced endocytic activity in macrophages via ClC-3

Isobavachalcone reveals novel characteristics of methuosis-like cell death in leukemia cells

Rapid gating and anion permeability of an intracellular aquaporin

Functional colocalization of water channels and proton pumps in endosomes from kidney proximal tubule

Macropinocytosis as a key determinant of peptidomimetic uptake in cancer cells

Differential signaling during macropinocytosis in response to M-CSF and PMA in macrophages

Dorsal ruffles enhance activation of Akt by growth factors

Giant vacuoles observed in Dictyostelium discoideum

Sodium channels are required during in vivo sodium chloride hyperosmolarity to stimulate increase in intestinal endothelial nitric oxide production

Second messengers regulate endosomal acidification in Swiss 3T3 fibroblasts

Water-transporting proteins

Cotransport of water by Na(+)-K(+)-2Cl(-) cotransporters expressed in Xenopus oocytes: NKCC1 versus NKCC2

SARs of a novel series of s-triazine compounds targeting vimentin to induce methuotic phenotype

MCOLN1 is a ROS sensor in lysosomes that regulates autophagy

Modulation of macropinocytosis-mediated internalization decreases ocular toxicity of antibody-drug conjugates

TMEM206 promotes the malignancy of colorectal cancer cells by interacting with AKT and extracellular signal-regulated kinase signaling pathways

Comparisons between graphene oxide and graphdiyne oxide in physicochemistry biology and cytotoxicity

Activin A downregulates the phagocytosis of lipopolysaccharide-activated mouse peritoneal macrophages in vitro and in vivo

Mechanism of Platycarya strobilacea Sieb. et Zucc extract-induced methuosis in human nasopharyngeal carcinoma CNE1 and CNE2 cells

Serumand glucocorticoid-inducible kinase-1 (SGK-1) plays a role in membrane trafficking in Caenorhabditis elegans

A tale of two CLCs: biophysical insights toward understanding ClC-5 and ClC-7 function in endosomes and lysosomes

Necrotic death as a cell fate

Ras and Rap1: two highly related small GTPases with distinct function