key: cord-0721060-6gmadi3h authors: Trassante, Carla Marcelino; Barboza, Victor dos Santos; Rocha, Liziane dos Santos; Correa, Paulo Maximiliano; Luchese, Cristiane; Wilhelm, Ethel Antunes; Pereira de Pereira, Claudio Martin; Baldissera, Matheus Dellaméa; Rech, Virginia Cielo; Giongo, Janice Luehring; de Almeida Vaucher, Rodrigo title: Detection of SARS-CoV-2 virus using an alternative molecular method and evaluation of biochemical, hematological, inflammatory, and oxidative stress in healthcare professionals date: 2021-05-19 journal: Microb Pathog DOI: 10.1016/j.micpath.2021.104975 sha: ed90ce043db240ce3b0a0094f08d3f7702b4d70f doc_id: 721060 cord_uid: 6gmadi3h In early December 2019, an outbreak of coronavirus disease 2019 caused by a new strain of coronavirus (SARS-CoV-2), occurred in the city of Wuhan, Hubei Province, China. On January 30, 2020, the World Health Organization (WHO) declared the outbreak a public health emergency of international concern. Since then, frontline healthcare professionals have been experiencing extremely stressful situations and damage to their physical and mental health. These adverse conditions cause stress and biochemical, hematological, and inflammatory changes, as well as oxidative damage, and could be potentially detrimental to the health of the individual. The study population consisted of frontline health professionals working in BHU in a city in southern Brazil. Among the 45 participants, two were infected with the SARS-CoV-2 virus and were diagnosed using immunochromatographic tests such as salivary RT-LAMP and qRT-PCR. We also evaluated biochemical, hematological, inflammatory, and oxidative stress markers in the participants. The infected professionals (CoV-2-Prof) showed a significant increase in the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol, lactic dehydrogenase, lymphocytes, and monocytes. In this group, the levels of uric acid, triglycerides, leukocytes, neutrophils, hemoglobin, hematocrit, and platelets decreased. In the group of uninfected professionals (NoCoV-2-Prof), significant increase in HDL levels and the percentages of eosinophils and monocytes, was observed. Further, in this group, uric acid, LDH, triglyceride, and cholesterol levels, and the hematocrit count and mean corpuscular volume were significantly reduced. Both groups showed significant inflammatory activity with changes in the levels of C-reactive protein and mucoprotein. The NoCoV-2-Prof group showed significantly elevated plasma cortisol levels. To our kowledge, this study is the first to report the use of the RT-LAMP method with the saliva samples of health professionals, to evalute of SARS-CoV-2. Introduction 63 The emergence of viral diseases poses a serious threat to global public health. Several Table 2 shows the results of the immunological and molecular tests performed for the 235 detection of SARS-CoV-2 in the study individuals. Table 3 shows the results of biochemical analysis. A significant reduction in the levels Oxidative stress was assessed using FRAP, TOS, TAC, and the OSI assay. Figure 4A 261 shows the levels of antioxidants in the serum for the groups evaluated using the FRAP assay. levels in the NoCoV-2-Prof group compared to the No-Prof group. Figure 4D shows the 267 results of the OSI. 268 Table 4 shows the results of the hemograms. 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Blood Cells Mol Dis Table 1: Socio-demographic data of the individuals studied NoCoV Data are expressed as mean ± SD of the NoCoV-2-Prof and CoV-2-Prof groups compared to the No-Prof