key: cord-0720352-lpvsvovh
authors: Amiano, Nicolás O.; Pellegrini, Joaquín M.; Morelli, María P.; Martinena, Camila; Rolandelli, Agustín; Castello, Florencia A.; Casco, Nicolás; Ciallella, Lorena M.; de Casado, Graciela C.; Armitano, Rita; Stupka, Juan; Gallego, Claudio; Palmero, Domingo J.; García, Verónica E.; Tateosian, Nancy L.
title: Circulating Monocyte-Like Myeloid Derived Suppressor Cells and CD16 Positive Monocytes Correlate With Immunological Responsiveness of Tuberculosis Patients
date: 2022-03-14
journal: Front Cell Infect Microbiol
DOI: 10.3389/fcimb.2022.841741
sha: 33f632dace667940e46e661140f742c58166c99b
doc_id: 720352
cord_uid: lpvsvovh

Alterations of myeloid cell populations have been reported in patients with tuberculosis (TB). In this work, we studied the relationship between myeloid-derived suppressor cells (MDSC) and monocytes subsets with the immunological responsiveness of TB patients. Individuals with active TB were classified as low responders (LR-TB) or high responders (HR-TB) according to their T cell responses against a cell lysate of Mycobacterium tuberculosis (Mtb-Ag). Thus, LR-TB, individuals with severe disease, display a weaker immune response to Mtb compare to HR-TB, subjects with strong immunity against the bacteria. We observed that LR-TB presented higher percentages of CD16 positive monocytes as compared to HR-TB and healthy donors. Moreover, monocyte-like (M-MDSC) and polymorphonuclear-like (PMN-MDSC) MDSC were increased in patients and the proportion of M-MDSC inversely correlated with IFN-γ levels released after Mtb-Ag stimulation in HR-TB. We also found that LR-TB displayed the highest percentages of circulating M-MDSC. These results demonstrate that CD16 positive monocytes and M-MDSC frequencies could be used as another immunological classification parameter. Interestingly, in LR-TB, frequencies of CD16 positive monocytes and M-MDSC were restored after only three weeks of anti-TB treatment. Together, our findings show a link between the immunological status of TB patients and the levels of different circulating myeloid cell populations.

Tuberculosis (TB) represents one of the main health global problems. The bacterial pathogen Mycobacterium tuberculosis (Mtb) was the second leading infectious killer after SARS-CoV-2 in 2020 (World Health Organization, 2021) . Worldwide, an estimated 10.0 million people fell ill with TB and there were 1.5 million died because of this disease in 2020 ( World Health Organization, 2021) . Despite the use of the bacilli Calmette-Gueŕin (BCG) vaccine and the current chemotherapeutic treatments, TB remains as the top 13 th causes of death around the world (World Health Organization, 2021) . Thus, one of the main challenges in developing new strategies to fight TB is focused on the reduction of the duration and complexity of drug regimens (World Health Organization, 2021) . Therefore, there is a critical need to understand the mechanisms of immune control to achieve a significant impact on the epidemic (Scriba et al., 2008) .

After infection, Mtb interacts with different cells of both, innate and adaptive immune compartments. These cells play an important role in the modulation and the development of the pathology (Bussi and Gutierrez, 2019 ). An efficient host protection against Mtb infection is associated with the induction, activation and proliferation of Th1 and Th17 cells (Jurado et al., 2012; du Plessis et al., 2013; Tateosian et al., 2017) , whom promote the release of cytokines such as IL-2, TNF-a, ; and the activation of effector monocytes (Jurado et al., 2012; du Plessis et al., 2013; Tateosian et al., 2017) . Moreover, it has been demonstrated that reduced IFN-g production is a marker of severe disease (Gong et al., 1996) . Nevertheless, how Mtb is able to evade host immune surveillance and persist, particularly inside myeloid cells, is not fully elucidated yet. Inflammatory myeloid cells are key players in the pathophysiology of TB (Dorhoi and Kaufmann, 2015) . In fact, it has been proposed that the phenotype of the populations of myeloid cells involved in early granuloma formation may influence substantially the progression of TB and the outcome of the infection (du Plessis et al., 2013; Guirado et al., 2013; Khan et al., 2019) . Many studies have shown that Mtb can also affect the differentiation of progenitors and immature myeloid cells, the activation of recruited macrophages and shape the cytokine profile produced by them (Hickman et al., 2002; Khan et al., 2019) . Furthermore, we have previously demonstrated that IFN-g and IL-17A differentially regulate the autophagy process in Mtb-infected monocytes derived from TB patients in correlation with the severity of the disease (Rovetta et al., 2014; Tateosian et al., 2017) . Phenotypically and functionally different subsets of monocytes were identified based on the relative expression of CD14 (co-receptor for toll-like receptor 4) and CD16 (Fc gamma receptor IIIa) (Ziegler-Heitbrock et al., 2010) . Three subpopulations of human monocytes have been defined: classical (CD14 ++ CD16 -), intermediate (CD14 ++ CD16 + ) and non-classical (CD14 + CD16 ++ ), which may represent different stages of maturation (Ziegler-Heitbrock et al., 2010) . During TB infection, CD16 + monocytes are expanded and do not differentiate into macrophages due to limited expression of maturation and differentiation markers such as CD11b, CD11c, CD33, and CD36 (Castaño et al., 2011) . Besides, it has been described that the ability of circulating CD16 + monocytes to differentiate into dendritic cells and induce T-cell activation is decreased in TB patients (Balboa et al., 2011) .

Myeloid-derived suppressor cells (MDSC) are another relevant population of phagocytes that participate in TB immunopathogenesis (Magcwebeba et al., 2019) . Although little is known about the role of MDSC, accumulating evidence clearly demonstrates their capacity to suppress T-cell responses in TB (Magcwebeba et al., 2019) . Moreover, MDSC have been reported to be linked to disease progression (du Plessis et al., 2013; Knaul et al., 2014; Tsiganov et al., 2014) . MDSC described in TB mainly comprise two different subsets: monocyte-like MDSC (M-MDSC) and polymorphonuclear-like MDSC (PMN-MDSC) cells (Bronte et al., 2016; Kotzéet al., 2020) . Although no specific markers have been described for MDSC identification (Magcwebeba et al., 2019) , human M-MDSC were shown to express CD14, CD33, and CD11b with a lack of CD15 and low or no HLA-DR expression [CD11b + HLA-DR -/low (CD14 + )] (Damuzzo et al., 2015; Bronte et al., 2016; Cassetta et al., 2019; Kotzéet al., 2020; Grassi et al., 2021) . On the other hand, PMN-MDSC express CD15, CD33 and CD11b in the absence of CD14 and low or no HLA-DR expression [CD15 + (CD14 -CD11b + )] (Damuzzo et al., 2015; Bronte et al., 2016; Cassetta et al., 2019; Kotzéet al., 2020; Grassi et al., 2021) .

The accumulation of MDSC during mycobacterial infections was first reported in mice models (Dietlin et al., 2007; Martino et al., 2010) . Furthermore, the expansion of MDSCs was observed both in blood and in pleural fluid of patients with pulmonary or extra-pulmonary TB (du Plessis et al., 2013) . Besides, it was also described a clear decrease of circulating MDSC frequencies at the end of successful anti-TB treatments (du Plessis et al., 2013) .

Due to the important role of monocytes and MDSC during human Mtb infection, in this study, we hypothesized that the levels of these circulating myeloid cells in TB patients could be related to their immunological parameters and the influence of anti-TB therapy. Thus, we found a differential expansion profile of monocytes and MDSC according to the immunological status of TB patients. We could observe that low responder TB patients (LR-TB) presented higher percentages of circulating CD14 + + CD16 + and CD14 + CD16 ++ monocytes and M-MDSC as compared to high responder patients (HR-TB) and healthy donors (HD). Furthermore, levels of CD14 ++ CD16 + and CD14 + CD16 ++ monocytes and M-MDSC were restored to normal after the first three weeks of anti-TB treatment in LR-TB. Therefore, we could show a relationship between the immunological status of TB patients and the frequencies of circulating CD16 positive monocytes and M-MDSC, suggesting that could be a new criterion to establish the immunological classification of TB patients. Taking into account the crucial role of cellular immunity during TB, these findings could be important pieces to better understand Mtb infection and TB disease.

All individuals who participated in this study (HD and TB) had been vaccinated with BCG according to Argentine regulations. Patients with TB were diagnosed at Dr. F. Muñiz or Dr. P. Piñero Hospitals (Buenos Aires, Argentina) based on clinical and radiological data together with the identification of acid-fast bacilli (AFB) in sputum. First peripheral blood samples were collected between 1 and 7 days of anti-tuberculosis (anti-TB) therapy administration and second samples were obtained between 14 and 21 days of anti-TB treatment. All patients were treated with anti-TB regular therapy for drug sensitive Mtb strains, according to WHO 

The exclusion criteria were carried out as previously described (Pellegrini et al., 2021) . Briefly, individuals participating of the study were 18 -60 years old and had no history of diseases affecting the immune system, such as HIV infection, treatment with immunosuppressive drugs, a recent diagnosis of cancer, hepatic or renal disease, pregnancy, or positive serology for other viral (e.g., hepatitis A, B or C), or bacterial (e.g., leprosy, syphilis) infections.

Individuals with latent infection were excluded from the present study by using the QuantiFERON-TB Gold Plus kit (Qiagen, Germany, USA).

TB Patients were classified as high responders (HR-TB) or low responders (LR-TB), based on their in vitro lymphocyte responses to a whole cell lysate of M. tuberculosis (Mtb-Ag) as previously described (Pasquinelli et al., 2004) . Briefly, HR-TB patients are individuals displaying significant proliferative responses, IFN-g production and an increased percentage of SLAMF1 + CD3 + cells after Mtb-Ag stimulation; whereas LR-TB patients exhibit low proliferative responses, IFN-g release and SLAMF1 + CD3 + cells. LR-TB patients had more severe pulmonary disease compared with HR individuals. Cut-off values to differentiate between LR-TB and HR-TB were established previously by Pasquinelli et al (Pasquinelli et al., 2004) . The fulfillment of two of these three criteria was sufficient to assign a patient to the corresponding group.

In vitro stimulation of fresh peripheral blood mononuclear cells (PBMC) was performed with a cell lysate from the virulent Mycobacterium tuberculosis strain H37Rv, prepared by probe sonication (Mtb-Ag) (BEI Resources, NIAID, NIH: Mtb, Strain H37Rv, Whole Cell Lysate, NR-14822).

All experiments were performed with fresh PBMC isolated by centrifugation on Ficoll-Hypaque (Amersham Biosciences, NJ, USA). Flow cytometry assays were performed after PBMC were washed with PBS plus 1% BSA and 0.1% NaN 3 and resuspended in staining buffer [PBS plus 1% Fetal Bovine Serum (FBS)]. To evaluate cell viability, propidium iodide (PI) method was used in all experiments. PBMC were also cultured (1 × 10 6 cells/mL), with or without Mtb-Ag (10 mg/mL) with RPMI 1640 medium (Gibco, MD, USA) supplemented with 1% L-glutamine, 1% penicillin/streptomycin, and 10% FBS (Gibco, MD, USA) during 48 h. Monocyte absolute number was evaluated by routine blood count.

Fresh PBMC from TB patients and HD were stained with specific fluorophore-marked antibodies against CD14 (FITC, clone HCD14, BioLegend, USA) and CD16 (APC, clone 3G8, BioLegend, USA) for differentiating monocyte subsets. Evaluation of MDSC percentage was performed using PBMC stained with specific fluorophore-marked antibodies against CD14 (FITC, clone HCD14, BioLegend, USA), CD11b (PE, clone ICRF44, BioLegend, USA), CD15 (PE/Cy7, clone HI98, BioLegend, USA), and HLA-DR (APC, clone L243, BioLegend, USA). Negative control samples were incubated with irrelevant isotype matched monoclonal antibody (FITC Mosue IgG1k, APC Mouse IgG1k, PE/Cy7 Mouse IgMk and APC Mouse IgG2a k isotype ctrl a ntibodies, BioLegend, USA) (Supplementary Figure 1) . Cell viability was evaluated by PI method. All samples were analyzed on a FACSAria II flow cytometer (BD Biosciences, CA, USA).

The levels of IFN-g were evaluated in supernatants using a commercial ELISA kit (Human IFN-g ELISA MAX Standard Kit, BioLegend, USA) following the manufacturer´s instructions.

IFN-g index: Fresh PBMC were stimulated with Mtb-Ag for 48h and then supernatants were obtained. IFN-g was measured by ELISA and the index for each individual was calculated as (pg/ mL IFN-g after Mtb-Ag stimulation)/(pg/mL IFN-g after culturing with medium).

Fresh PBMC were stimulated with Mtb-Ag for five days and then, cells were pulsed with [ 3 H]TdR (1 mCi/well) and harvested 16 hours later. [ 3 H]TdR incorporation was measured in a liquid scintillation counter as counts per minute (c.p.m). Proliferation index for each individual was calculated as (c.p.m. after Mtb-Ag stimulation)/(c.p.m. after culturing with medium).

M-MDSC from TB patients were obtained from fresh PBMC by cell sorting (FACS). Cells were labeled with anti-CD14 (FITC, clone HCD14, BioLegend, USA), anti-CD11b (PE, clone ICRF44, BioLegend, USA) and anti-HLA-DR (APC, clone L243, BioLegend, USA) and then sorted via a FACSAria II flow cytometer (BD Biosciences, CA, USA). FACS-sorted M-MDSC were co-cultured with autologous lymphocytes (ratio 1:1) and treated with anti-CD3 (0.1µg/mL, clone OKT3, Biolegend, USA) and anti-CD28 (0.5µg/mL, clone CD28.8, Biolegend, USA) antibodies. Stimulated lymphocytes without adding M-MDSC were used as control. Each condition was run in triplicate. Then supernatants were collected after 48h and IFN-g was measured by ELISA. Relative IFN-g release was calculated to Control (100%). Furthermore, proliferation was measured by pulsing cultured cells with [ 3 H]TdR (1 mCi/well) at day 5 and harvested 16 hours later. [ 3 H]TdR incorporation was measured in a liquid scintillation counter as counts per minute (c.p.m). Relative proliferation was calculated to Control (100%).

Analysis of variance (ANOVA) and post hoc Tukey´s multiple comparisons test were used as indicated in figure legends. The Mann-Whitney U test and the Wilcoxon matched-pairs signedrank were used to analyze differences between groups. For categorical variables, the Chi-square (and Fisher´s exact) test for homogeneity was performed to compare proportions of subjects between groups. In the indicated graphs each symbol represents an individual and the horizontal lines indicate the mean ± standard deviation (SD). Correlations were calculated using the non-parametric Spearman correlation test. Receiver operating characteristic (ROC) curve analysis was performed to analyze the predictive value of the frequencies of CD14 ++ CD16 + , CD14 + CD16 ++ and M-MDSC cells populations, calculating the area under the curve (AUC) and the 95% confidence interval (CI). Analyses were performed using GraphPad Prism 8.0.2 software. P < 0.05 was considered statistically significant.

We initially investigated the subsets of circulating blood monocytes in pulmonary TB patients classified according to their immunological responsiveness against Mtb-Ag. As previously described in other populations, TB patients showed higher percentage of circulating CD16 + monocytes than healthy BCG-vaccinated donors (HD) (TB = 10.1% ± 1.9; HD = 5.4% ± 0.8; Mean ± SD. *P < 0.05, Mann-Whitney U test). Furthermore, percentages of classical (CD14 ++ CD16 -), intermediate (CD14 + + CD16 + ) and non-classical (CD14 + CD16 ++ ) monocytes were also analyzed in two groups of TB patients classified as high responders (HR-TB) or low responders (LR-TB) based on their in vitro lymphocyte responses against Mtb-Ag as previously described (Pasquinelli et al., 2004) . Demographic characteristics, clinical and immunological parameters of the studied populations are shown in (Table 1) . Our results showed no perturbation of classical monocytes percentages when comparing LR-TB, HR-TB and HD ( Figure 1A) . On the other hand, we found that LR-TB patients presented the highest percentages of CD14 ++ CD16 + ( Figure 1B ) and CD14 + CD16 ++ ( Figure 1C ) circulating cells as compared to HR-TB and HD subjects. Moreover, HR-TB patients showed similar frequencies of CD14 ++ CD16 + and CD14 + CD16 ++ to HD. Representative flow cytometry plots are shown in Figure 1D . Nevertheless, it is important to mention that the analysis of total monocyte count in peripheral blood showed no differences between LR-TB and HR-TB (Supplementary Figure 2) . The HR-TB and LR-TB subpopulations of patients were characterized on their in vitro lymphocyte responses against Mtb-Ag as previously described in the Methods section. Figures 1E-G show differential levels of IFN-g, proliferation index and percentages of SLAMF1 + CD3 + cells in the two groups of TB patients and HD after stimulating their PBMC with the Mtb-Ag. In view of our results, and to evaluate the potential use of CD14 ++ CD16 + and CD14 + CD16 ++ cells frequencies as new criteria to discriminate individuals LR-TB from HR-TB, we performed ROC analyses. From this study, significant results were obtained for CD14 ++ CD16 + cells (AUC =0.9091; P < 0.01; 95% CI: 0.7788-1.000) and for CD14 + CD16 ++ cells (AUC =0.8500; P < 0.01; 95% CI: 0.6437-1.000), demonstrating that the percentages of circulating intermediate and non-classical monocytes allow differentiating between these patients with distinct immunological status. A cut-off value of 5.0% for CD14 ++ CD16 + cells allowed a differentiation with a Sensitivity =87.5% and a Specificity =81.8%. Moreover, for CD14 + CD16 ++ a cut-off value of 5.2% allowed a differentiation with a Sensitivity =75.0% and a Specificity =100%. Therefore, the analysis of the different monocyte subsets could serve as new criteria for the immunological classification of TB patients.

Then, we also decided to compare the frequencies of circulating MDSC in our study population. We Figures 3A, B) . To further investigate the clinical significance of MDSC in TB disease, we studied whether LR-TB, HR-TB and HD presented different levels of circulating M-MDSC and PMN-MDSC. The FACS analysis showed higher levels of M-MDSC in LR-TB in comparison with HR-TB and HD individuals (Figure 2A) . On the contrary, HR-TB patients presented the highest levels of PMN-MDSC compared to LR-TB and HD ( Figure 2B ). Representative flow cytometry plots are shown in Figure 2C (M-MDSC) and Figure 2D (PMN-MDSC). Then, due to the importance of IFN-g in the immune response against Mtb, we evaluated the possibility of a correlation between percentages of MDSC and the IFN-g index in the two TB patient populations. The performed analysis did not show a correlation in the percentages of PMN-MDSC for any of the TB patient sub-populations. Furthermore, no correlation for LR-TB was observed between M-MDSC and IFN-g index; however, it was observed that the percentage of M-MDSC negatively correlated with IFN-g index in HR-TB patients. ( Figure 2E) . These results suggested an immunosuppressive role of M-MDSC on TB patients´lymphocytes. To corroborate this, functional experiments were performed. We investigated the suppressive potential of M-MDSC from recently diagnosed active TB patients on T-cell function by analyzing proliferation and IFN-g production. Co-culture experiments showed that sorted CD14 + CD11b + HLA-DR -/low from TB patients exhibit the ability to suppress polyclonally stimulated (anti-CD3, anti-CD28) autologous lymphocytes proliferation and the production of IFN-g (Supplementary Figures 4A, B) . Thus, we could confirm the suppressive potential of these M-MDSC from TB patients.

Moreover, in order to assess the potential use of circulating M-MDSC frequencies as a new criterion for discriminating between LR-TB and HR-TB, we next performed a ROC analysis, which showed significant results (AUC =0.7857; p < 0.05; 95% CI: 0.58-0.99; Cut-off =11.0%; Sensitivity =75.0%; Specificity =85.7%). This demonstrated that, together with the percentages values observed for circulating intermediate and non-classical monocytes, M-MDSC frequencies could be used also as another immunological classification parameter of disease severity.

It was previously described a significant reduction in MDSC frequencies in patients with TB at the end of antibiotic treatment; therefore, we decided to study whether that effect could be observed after a few weeks of therapy in TB patients with a diminished immune responsiveness against Mtb. We then compared percentages of blood circulating PMN-MDSC, M-MDSC and classical, intermediate and non-classical monocytes at the beginning and after three weeks of anti-TB treatment. No differences in the percentage of CD14 ++ CD16were observed after this treatment period in LR-TB patients. However, we observed a significant decrease in CD14 ++ CD16 + and CD14 + CD16 ++ monocyte levels after this short period of treatment ( Figures 3A-C) . In addition, in LR-TB patients, we observed a significant reduction of the frequency of M-MDSC ( Figure 3D ). In contrast, in the same group of patients the percentage of PMN-MDSC was not affected by the anti-TB treatment ( Figure 3E ). Representative flow cytometry plots are shown in Supplementary  Figure 5 . At the same time that these modifications were detected in LR-TB cell populations an increase in IFN-g production and proliferation index was also observed after PBMCs stimulation with Mtb-Ag ( Figures 3F, G) . However, no differences were detected in the percentage of SLAMF1 + CD3 + T cells ( Figure 3H ). It is important to point out that none of these parameters were modified during this short period of time in HR-TB patients.

Together, our present findings extend the knowledge about TB patient immunological parameters and how it is affected by a few days of anti-TB treatment. The accumulation of intermediate and non-classical monocytes and M-MDSC in LR-TB patients suggests a differential role of this cells populations in TB patients with a weaker immune response.

A spectrum of stages caused by Mtb infection leads to identify that some patients can control the bacterial infection and others cannot. The IFN-g producing Th1 cells are essential to control mycobacterial replication (Serbina et al., 2001; Salgame, 2005) . Indeed, reduced IFN-g production is a well-known marker of disease severity (Gong et al., 1996) . In our study population, HR-TB and LR-TB patients were identified based on their T cell responses against Mtb-Ag (Pasquinelli et al., 2004) . Th1 and Th17 cells alone do not explain the resistance/susceptibility to infection and disease (Forbes et al., 2008; Jurado et al., 2012) , suggesting that other actors might be required during the immune regulation of TB. Myeloid cells are a heterogeneous group of cells that plays a major role in the regulation of immune responses in many pathological conditions. However, the imbalance of myeloid cells during human TB has poorly been studied. Therefore, we aimed to investigate populations of monocytes and MDSC cells during human active TB disease. In the present report, we identified a specific profile in different circulating myeloid cell populations in TB patients. Our novelty data show that TB patients with with those recently described by Grassi et al. where an association between PMN-MDSC levels and the severity of TB disease, evaluated by chest X-ray, was demonstrated (Grassi et al., 2021) .

In that work, the authors show that the frequencies of PMN-MDSC are higher in those TB patients classified with a low/mild severity score compared to those classified with a high severity score. However, it is important to mention that this classification criterion does not take into account immunological parameters (Grassi et al., 2021) . We also observed for the first time, that the frequencies of CD14 ++ CD16 + and CD14 + CD16 ++ monocytes and M-MDSC return close to healthy control levels after a short time of anti-TB treatment (Figure 3) . Therefore, we showed that these Fresh PBMC from TB patients were stimulated with Mtb-Ag (10 µg/ml) for 48 h Afterwards, the production of IFN-g was determined in supernatants by ELISA and the IFN-g index was calculated as described. Correlation factor (r) and P values were calculated using the Spearman correlation test (LR-TB N = 12 and HR-TB N = 7). *P < 0.05.

cell subsets could contribute to a better characterization of the immunological profile of TB patients and could be new targets for the development of host-directed therapies. Previously, we have described that monocytes from LR-TB individuals were unable to activate the autophagy process through IL-17A at least in part because of a defect in the MAPK1/3 signaling pathway. In contrast, both IFN-g and IL-17A increased the levels of autophagy in patients with strong immunity to Mtb (Tateosian et al., 2017) . Moreover, circulating CD16 + monocytes and M-MDSC have been associated with clinical parameters of TB disease severity (Sampath et al., 2018; Jøntvedt Jørgensen et al., 2020) . Sampath et al., have found that CD16 positive monocytes are expanded in TB infection and perturbation of this subset defines the severity of TB (Sampath et al., 2018) correlated with TB disease severity by evaluating cavitary disease, erythrocyte sedimentation rate and monocyte:lymphocyte ratio among other parameters (Jøntvedt Jørgensen et al., 2020) . Furthermore, it has been previously described a positive correlation between PMN-MDSC frequencies and percentage of monocytes in Mtb infected subjects (TB and LTBI). However, this correlation was observed in CD14 ++ CD16but not in CD14 -CD16 ++ and CD14 ++ CD16 + monocytes (Grassi et al., 2021) . Our present results extended those findings, demonstrating that LR-TB patients, individuals with weakened immune response (Pasquinelli et al., 2004; Jurado et al., 2012) , showed higher proportions of intermediate and non-classical monocytes and M-MDSC in peripheral blood as compared to HR-TB patients, individuals with a strong immunity in response to Mtb-Ag. Thus, the proportion of circulating CD14 ++ CD16 + and CD14 + CD16 ++ monocytes and M-MDSC in patients with active TB might be also reflecting the immunological and clinical severity of the disease.

In accordance with previous reports (du Plessis et al., 2013; Zhan et al., 2018) , we found that MDSC are expanded in peripheral blood of active TB patients (Supplementary Figure 3) . Moreover, we show for the first time that there is a characteristic expansion profile of M-MDSC and PMN-MDSC in TB patients related to their immunological status and responsiveness. Our results in fresh PBMC confirmed that the majority of HR-TB patients present augmented percentages of PMN-MDSC. Furthermore, as we mentioned before, patients with weak immunity to Mtb presented the highest levels of circulating M-MDSC. However, the inverse correlation between the percentages of circulating M-MDSC and IFN-g index was only observed in HR-TB patients (Figure 2) . These would suggest a marked suppressive effect of M-MDSC from HR-TB than cells from LR-TB. Moreover, these results are in agreement with those reported previously where a functional correlation between MDSC and TB infection was also suggested (Lee et al., 2012; du Plessis et al., 2013) . Several studies have recently reported the function and the accumulation of MDSC in association with pathogen load (du Plessis et al., 2013; Knaul et al., 2014) . du Plessis, et al. showed an increased frequency of MDSC during active TB with suppressed T cell functions (du Plessis et al., 2013) . Furthermore, in a mice model, Knaul, et al. described that MDSC are able to phagocyte Mtb, and released both, pro-inflammatory (IL-6, IL-1a) and immunomodulatory (IL-10) cytokines while retaining their suppressive capacity (Knaul et al., 2014) . Moreover, it was reported that transmembrane-TNF expressed on MDSC is crucial for its suppressive activity that regulates the inflammatory process associated with Mtb infection (Chavez-Galan et al., 2017) . Both, HR-TB and LR-TB patients showed a similar number of AFB in sputum smear, indicating no differences in the bacterial load between them. This could suggest that the immune microenvironment generated during the chronic inflammation of Mtb infection might influence the accumulation and function of monocytes and MDSC.

Additionally, it has been previously shown that pharmacological therapy reduce the accumulation of the myeloid cell population (Sańchez et al., 2006; du Plessis et al., 2013; Zhan et al., 2018) . Sanchez et al. demonstrated that the expression of CD14, HLA-DR and CD36 was decreased in monocytes of TB patients (Sańchez et al., 2006) ; nevertheless, normal expression of these molecules was restored after 6 months of anti-TB treatment. Furthermore, in TB patients, the MDSC population was reduced at the end of anti-TB therapy (du Plessis et al., 2013; Zhan et al., 2018) . However, it is important to mention that in our study we could observe for the first time to our knowledge, a restoration in the circulating levels of CD14 ++ CD16 + and CD14 + CD16 ++ monocytes and M-MDSC after a short term (three weeks) of anti-TB treatment in LR-TB. In this short period, we did not observe differences in percentages of circulating CD14 ++ CD16or in PMN-MDSC levels. Further investigation in studies with larger patient numbers was required. Therefore, considering previously published data and our present results, we hypothesize that intermediate and non-classical monocytes and M-MDSC populations could serve as a treatmentresponse marker during TB. In addition, the circulating levels of CD14 ++ CD16 + and CD14 + CD16 ++ monocytes and M-MDSC, together with information about the immunological status of patients related to T cell responses, would contribute to differentiate more efficiently between LR-TB and HR-TB patients.

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

The studies involving human participants were reviewed and approved by Hospital F.J. Muñiz and Hospital P. Piñero ethics committes. "Estudio de los mecanismos inmunoloǵicos que operan durante la infeccioń humana por Mycobacterium tuberculosis conduciendo a enfermedad activa o contencioń del patoǵeno" (approved by the Ethics Committee of Hospital F.J. Muñiz) and "Desarrollo de un nuevo metodo de deteccioń de infeccioń con Mycobacterium tuberculosis basado en tetraḿeros CMH-peptidos de antıǵenos de latencia" (approved by the Ethics Committe of Hospital P. Piñero). The patients/participants provided their written informed consent to participate in this study.

NT designed the study. NA, NT, JMP, MM, CM, AR, and FC were responsible for performing flow cytometry analysis, ELISA, and proliferation assays. NA was in charge of performing the QFT test in HD to evaluate latent infection. NA, NT, JMP, MM, and VG did the data management and analysis. NT, NA, JMP and MM prepared all the figures and tables. JMP, MM, AR, CM, and FC were responsible for processing samples and contributed with standard laboratory work. NA and NT wrote the manuscript. VG provided also expert advice. NC, LC, GC, RA, JS, CG, and DP were in charge of patient recruitment, diagnosis of active tuberculosis, and sample collection. All authors contributed to data gathering and interpretation, and revision of the report.

This work was supported by grants from Agencia Nacional de Promocioń Cientıfca y Tecnoloǵica (ANPCyT) (PICT2017-1158 and PICT2014-1709 to NT, PICT 2017-1451 to VG and PICT Start Up 2016-0022 to NA) and Universidad de Buenos Aires (UBACyT) (20020170100127BA to VG and 200201150200107BA to NA) .

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Mycobacterium Bovis Bacillus Calmette-Gueŕin Vaccination Mobilizes Innate Myeloid-Derived Suppressor Cells Restraining In Vivo T Cell Priming via IL-1R-Dependent Nitric Oxide Production

Expression of Signaling Lymphocytic Activation Molecule-Associated Protein Interrupts IFN-g Production in Human Tuberculosis

Neutrophil Autophagy During Human Active Tuberculosis Is Modulated by SLAMF1

IFNG-Mediated Immune Responses Enhance Autophagy Against Mycobacterium Tuberculosis Antigens in Patients With Active Tuberculosis

Host Innate and Th1 Responses and the Bacterial Factors That Control Mycobacterium Tuberculosis Infection

Monocyte Subsets: Phenotypes and Function in Tuberculosis Infection

Functional and Phenotypic Changes in Monocytes From Patients With Tuberculosis Are Reversed With Treatment

Distinct, Specific IL-17-and IL-22-Producing CD4+ T Cell Subsets Contribute to the Human Anti-Mycobacterial Immune Response

CD4(+) T Cells Are Required for the Development of Cytotoxic CD8(+) T Cells During Mycobacterium Tuberculosis Infection

IL17A Augments Autophagy in Mycobacterium Tuberculosis-Infected Monocytes From Patients With Active Tuberculosis in Association With the Severity of the Disease

Gr-1dimcd11b+ Immature Myeloid-Derived Suppressor Cells But Not Neutrophils Are Markers of Lethal Tuberculosis Infection in Mice

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IFN-g Decreased the Suppressive Function of CD33+HLA-DRlow Myeloid Cells Through Down-Regulation of PD-1/PD-L2 Signaling Pathway

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Conflict of Interest: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest

We thank to Guillermo Piazza, Agustina Erschen, Ceśar Salomone and Sergio Nemirovsky for their expert technical assistance.

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2022.841741/ full#supplementary-material