key: cord-0718250-axume2c0 authors: Tyrrell, D.A.J.; Alexander, D.J.; Almeida, J.D.; Cunningham, C.H.; Easterday, B.C.; Garwes, D.J.; Hierholzer, J.C.; Kapikian, A.; Macnaughton, M.R.; McIntosh, K. title: Coronaviridae: Second Report1 date: 2008-07-23 journal: Intervirology DOI: 10.1159/000148996 sha: e043f52258cae8e3a747fcbb6b5d9b51f4e50bad doc_id: 718250 cord_uid: axume2c0 nan ; HCV (OC43) [4] ; HEV [5] ; IBV [6] [7] [8] [9] ; TGEV [5] . Number of pieces: One or several [3] [4] [5] [6] [7] [8] [9] , Sedimentation coefficients: HCV (229E), 52S [3] ; HCV (OC43), 70S [4] ; HEV, 60-70S [5] ; IBV, 48-64S [6, 8, 9] ; TGEV, 60-70S [5] , Molecular weight: HCV (229E), 5.8 x 10s [3] ; HCV (OC43), 6.1 x 10° [4] ; IBV, 5.6-9.0 x 10° [6] [7] [8] [9] , Infectivity: IBV RNA is infectious [6, 8] . Other features: HCV (229E) contains a covalently attached poly(A) tract of about 70 nucleotides at or near the 3'-terminus [3] . HCV (OC43) contains a covalently attached poIy(A) tract of about 19 nucleotides [10] . IBV [6] [7] [8] and TGEV [11] also contain covalently attached poly(A) tracts. Proteins Number of polypeptides: HCV, 6 or 7 polypeptides [12, 13] ; HEV, 5 polypeptides [14] ; IBV, 7 polypeptides [15] ; MHV, 4-6 poly peptides [16] ; TGEV, 4-6 polypeptides [17] . Molecular weights of polypeptides: HCV, 15,000-196,000 [12, 13] ; HEV, 26,500-180,000 [14] ; IBV, 33,000-130,000 [15] ; MHV, 23,000-180,000 [16] ; TGEV, 28,000-200,000 [ 17] , Enzymes: RNA-dependent RNA polymerase not found in HCV (OC43) [10] or IBV [8] , There are unpublished reports that RNAand DNA-dependent RNA polymerases are not found in HCV or IBV. Other functional proteins: Hemagglutinin in HCV, IBV [18, 19] , HEV and NCDCV [20] , Hemagglutinin inhibited by specific anti sera. Lipids: TGEV contains phospholipid and glycolipid resembling those of host cell [21] , 2.1.4 Carbohydrates: Several peptides are glycosylated, probably the surface peptides: HCV [12, 13] ; HEV [14] ; IBV [15] ; MHV [22] ; TGEV [ [27] . CET: Diarrhea in turkeys. FIPV: Infectious peritonitis in cats [28] , HCV: Common colds in humans. HECV: Possibly diarrhea in humans [29] . HEV: Encephalitis in pigs. IBV: Acute respiratory disease, nephritis and gonadal damage in chickens. MHV: Acute hepatitis and/or encephalitis in mice, also chronic immunologically mediated diseases [30] . Coronavirus: a comparative review The genome of human coronavirus strain 229E The RNA of human coronavirus 0 0 4 3 Identification of heat-dissociable RNA complexes in two porcine coronaviruses Biological properties of avian coronavirus RNA The characterisation of the virion RNA of avian infectious bronchitis virus Presence of infectious polyadenylated RNA in the coronavirus avian bronchitis virus The ribonucleic acid of infectious bronchitis virus Presence of genomic polyadenylate and absence of detectable virion transcriptase in human coronavirus OC-43 Progress in coronaviruses Protein composition of coronavirus OC43 Purification and biophysical properties of human coronavirus 229E The polypeptides of hacmagglutinating encephalo myelitis virus and isolated subviral particles The polypeptide composition of avian in fectious bronchitis virus particles Characterization of a coronavirus. I. Structural proteins: effects of preparative conditions on the migration of protein in polyacrylamide gels The polypeptide structure of transmissible gastro enteritis virus Procedures for the haemagglutination and the haemagglutination inhibition tests for avian infectious bronchitis virus Haemagglutination by avian infectious bronchitis virus -a coronavirus Characterization of a calf diarrheal coronavirus Lipids of transmissible gastroenteritis virus and their relation to those of two different host cells Characterization of a coronavirus. II. Glycoproteins of the viral envelope: tryptic peptide analysis Isolation of subviral components from transmissible gastroenteritis virus Polypeptides of the surface projections and the ribonuclcoprotein of avian infectious bronchitis virus Ribonucleoprotein-like structures from coronavirus particles Isolation and morphology of the internal component of human coronavirus, strain 229E Studies on the structure of a coronavirus -avian infectious bronchitis virus Intestinal infection of neonatal dogs with canine coronavirus 1-71: studies by virologic, histologic, histochemical and immunofluorescent techniques Morphologic and physical characteristics of feline infectious peri tonitis and its growth in autochthonous peritoneal cell cultures Further studies on human enteric coronaviruses Neuropathological effects of persistent infection of mice by mouse hepatitis virus Lethal enteritis in infant mice caused by mouse hepatitis virus We wish to thank Dr. R.W. Bingham