key: cord-0717454-ubppy2s0
authors: Xu, Jun; Zhang, Yong; Zhao, Xiang; Wang, Dayan; Dai, Weiping; Jiao, Guangyu; Wang, Shiwen; Liu, Jun; Shi, Weifeng; Chen, Cao; Chen, Shuhong; Yan, Shichun; Hua, Hua; Gao, George F.; Xu, Wenbo; Wu, Guizhen
title: A Reemergent Case of COVID-19 — Harbin City, Heilongjiang Province, China, April 9, 2020
date: 2020-06-19
journal: China CDC Wkly
DOI: 10.46234/ccdcw2020.127
sha: c45d8213df22439f794b37a8cc47fd1f3f21ca4a
doc_id: 717454
cord_uid: ubppy2s0

nan

As of February 22, a total of 198 confirmed COVID-19 cases were reported in Harbin, Heilongjiang Province, and no new local cases were reported in Harbin over the next 54 days (1) . However, on April 9, a newly confirmed COVID-19 case was reported with an unknown source of infection (2) . The patient, a 54-year-old male, developed a fever on March 28 with the highest recorded temperature at 37.5 ℃. On April 7, the symptoms worsened and included dyspnea, cough, sputum, chest pain, and other symptoms, though he did not experience chest distress, heart palpitations, nausea and vomiting, abdominal pain or diarrhea. He went to the fever clinic of the Harbin Second Hospital and was admitted to the isolation ward with a temperature of 37.2°C. The patient also had a history of diabetes.

The results of a routine blood examination were normal (WBC 5.15×10 9 /L; lymphocyte 1.66×10 9 /L, lymphocyte percentage 32.3%; neutrophil percentage 51.9%, c-reactive protein 36.56 mg/L). Chest computed tomography (CT) showed multiple lymph node shadows in the mediastinum and multiple lamellar ground glass density shadows and mesh shadows in both lungs. On April 7, the throat swabs of the patients were tested by Harbin CDC by using realtime reverse transcription-polymerase chain reaction (real-time RT-PCR) targeting the ORF 1ab and N genes of COVID-19 virus, and the results showed that the nucleic acid of COVID-19 virus of the throat swab was positive. On April 8, Harbin CDC tested his pharyngeal swabs, blood, urine and stool samples again. COVID-19 nucleic acids were detected, but the test for serum antibodies for COVID-19 was negative.

The National Institute for Viral Disease Control and Prevention (IVDC) of China CDC conducted wholegenome sequencing analysis on a pharyngeal swab sample of the patient, which showed 99.97% nucleic acid similarity to the reference strain EPI_ISL_402119, which was isolated from Wuhan on January 7, 2020.

There was a total of eight nucleotide mutations between them. This strain possessed the nucleotide mutations C241T, C1059T, C3037T, C14408T, A23403G, and G25563T, which were consistent with the characteristics of the North American branch of the L lineage from Europe ( Figure 1) (3). In addition, sequence alignment also revealed two mutations C26636T and C27213T in the virus genome from this patient. Among them, C1059T, C14408T, A23403G, and G25563T caused amino acid substitutions, which were located on 5'-UTR, ORF1ab, S protein, and ORF3a, respectively. This means that despite amino acid mutations in the virus, its biological characteristics, including its ability to bind to its receptor have not changed significantly.

Epidemiological investigations showed that the patient had no travel, residence, or stay history in Wuhan City and surrounding areas or other communities with confirmed COVID-19 case 14 days before the onset of disease (March 14-March 28). There was no contact with people with fever or respiratory symptoms from Wuhan City and surrounding areas nor from communities with confirmed case reports, nor did the patient have a history of overseas travel.

However, because this patient did not seek medical treatment and isolation immediately after the onset of fever, he still had normal social activities including holding a dinner party. Due to the prevalence of asymptomatic COVID-19 infection, epidemiological investigation and virus tracking surrounding this case became more challenging. Although the local transmission of the COVID-19 virus in the mainland of China had been interrupted, COVID-19 is still an epidemic in many other countries. Many cases of COVID-19 and asymptomatic infections entering China through airports and land ports have been detected (4). Therefore, it is necessary to strengthen COVID-19 surveillance in respiratory tract and stool

Harbin Municipal People's Government. COVID-19 Situation in Harbin

Harbin Municipal People's Government. COVID-19 Situation in Harbin

On the origin and continuing evolution of SARS-CoV-2

Active case finding with case management: the key to tackling the COVID-19 pandemic