key: cord-0715941-i99lqr0p authors: Espenhain, Laura; Funk, Tjede; Overvad, Maria; Edslev, Sofie Marie; Fonager, Jannik; Ingham, Anna Cäcilia; Rasmussen, Morten; Madsen, Sarah Leth; Espersen, Caroline Hjorth; Sieber, Raphael N.; Stegger, Marc; Gunalan, Vithiagaran; Wilkowski, Bartlomiej; Larsen, Nicolai Balle; Legarth, Rebecca; Cohen, Arieh Sierra; Nielsen, Finn; Lam, Janni Uyen Hoa; Lavik, Kjetil Erdogan; Karakis, Marianne; Spiess, Katja; Marving, Ellinor; Nielsen, Christian; Wiid Svarrer, Christina; Bybjerg-Grauholm, Jonas; Olsen, Stefan Schytte; Jensen, Anders; Krause, Tyra Grove; Müller, Luise title: Epidemiological characterisation of the first 785 SARS-CoV-2 Omicron variant cases in Denmark, December 2021 date: 2021-12-16 journal: Euro Surveill DOI: 10.2807/1560-7917.es.2021.26.50.2101146 sha: afec285cca8d74127ba524638375e0b17930adfb doc_id: 715941 cord_uid: i99lqr0p By 9 December 2021, 785 SARS-CoV-2 Omicron variant cases have been identified in Denmark. Most cases were fully (76%) or booster-vaccinated (7.1%); 34 (4.3%) had a previous SARS-CoV-2 infection. The majority of cases with available information reported symptoms (509/666; 76%) and most were infected in Denmark (588/644; 91%). One in five cases cannot be linked to previous cases, indicating widespread community transmission. Nine cases have been hospitalised, one required intensive care and no deaths have been registered. By 9 December 2021, 785 SARS-CoV-2 Omicron variant cases have been identified in Denmark. Most cases were fully (76%) or booster-vaccinated (7.1%); 34 (4.3%) had a previous SARS-CoV-2 infection. The majority of cases with available information reported symptoms (509/666; 76%) and most were infected in Denmark (588/644; 91%). One in five cases cannot be linked to previous cases, indicating widespread community transmission. Nine cases have been hospitalised, one required intensive care and no deaths have been registered. On 26 November 2021, the World Health Organization designated the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant (Phylogenetic Assignment of Named Global Outbreak (Pango) lineage designation B.1.1.529) a variant of concern [1] . Two days later, on 28 November 2021, the first two Omicron cases were identified in Denmark in travellers returning from South Africa (see detailed timeline in Table 1 ). By 9 December, a total of 785 Omicron cases have been registered in Denmark. The aim of this communication is to describe these first Danish cases to provide insights into the spread and early indications of severity. An important pillar in the Danish coronavirus disease (COVID- 19) response has been free and easy access to testing. Community testing is carried out in 'TestCenter Denmark' (TCDK), where every resident or visitor in Denmark can book an appointment (TCDK carried out more than 85% of all tests [2] and uses reverse transcription PCR (RT-PCR) for SARS-CoV-2 confirmation). Furthermore, testing is also carried out through referral mainly from general practitioners or hospitals as part of the national healthcare testing system, for which samples are analysed in one of 10 local clinical microbiology departments using RT-PCR or other nucleic acid amplification tests. The testing strategy is supplemented by an extensive antigen-testing programme, and persons with a positive antigen test are encouraged to get a confirmatory PCR test. The Danish COVID-19 testing strategy includes asymptomatic testing for close contacts of confirmed cases and screening tests in certain settings (e.g. workplaces and primary schools). Overall, more than 15% of the Danish population is PCR-tested at least once during a week [3] . By 9 December, 9.2% of the Danish population had tested positive for SARS-CoV-2 since the start of the epidemic. Surveillance of SARS-CoV-2 variants in Denmark is based on screening of all positive samples with variantspecific RT-PCR and extensive whole-genome sequencing (WGS) efforts. For most of 2021, more than 90% of SARS-CoV-2 isolates were sequenced, although rates have been lower since late autumn because of rising case numbers [4] . The TCDK has a maximum capacity of 15,000 WGS per week. An RT-PCR detecting the Omicron variant using the 452L marker (estimated specificity: 99.99% based on retrospective analysis) [5] was developed and implemented in the TCDK by 1 December. In the 10 local clinical microbiology departments, similar solutions have been set up. All isolates from cases who are found to be RT-PCR-positive for the Omicron variant are subsequently subjected to WGS. More WGS data are needed in order to have a meaningful positive predictive value. A confirmed Omicron case was defined based on WGS results until 3 December. From 4 December, the definition was extended to also include positive RT-PCR for the variant. Extensive contact tracing around Omicron cases was initiated and is carried out by the Danish Patient Safety Authority (STPS). This includes self-isolation of cases until 48 h after symptom cessation for confirmed cases or, if asymptomatic, self-isolation for 7 days. It is recommended that all close contacts, as well as their close contacts, regardless of vaccination status, are tested (on Day 1, 4 and 6 after last contact with an Omicron case) and self-quarantine until a negative test result has been received after the final test on Day 6. Self-quarantine of close contacts of close contacts was abolished on 7 December 2021. Restrictions on incoming travellers were introduced on 27 November (Table 1) . To describe the Omicron cases, we used data from the routine Danish surveillance of COVID-19 [6] in which information from several national registries is linked daily. This provides information on demographic characteristics, vital status, vaccination status, previous SARS-CoV-2 infection, admission to hospital and intensive care treatment of cases. During contact tracing, the STPS collects information on travel history symptoms and suspected place of infection. As per 9 December 2021, 785 cases of SARS-CoV-2 Omicron have been registered in Denmark. As there is a few days' lag from sampling to test result, the latest sampling date was 7 December. Daily case counts rose quickly ( Figure 1) , with daily increases of more than 40% from 4 December. In total, 143 (18%) have so far been confirmed by WGS. The age of cases ranged between 2 and 95 years (median: 32) and 433 (55%) were male ( Table 2 for reference. As at 9 December, 644 cases had known information on place of infection (82% of all cases) and 56 of them (8.7%) reported a travel history. Sixteen reported travelling from South Africa, but also other African and European countries were reported (Table 2) . These introductions are apparent on the phylogenetic tree ( Figure 2 ). In addition, 464 cases (75%) reported a possible place of infection and 180 non-travellers (28%) did not report a suspicion on where they had been infected. To date, STPS has linked at least 83 Omicron cases to five large events in Denmark (> 100 participants). The number of cases related to these events is expected to increase as testing and contract tracing are ongoing. A high attack rate was reported at one of the events, a seasonal gathering with 150 participants, where 71 (47%) participants got infected. The SARS-CoV-2 Omicron variant was introduced to the gathering by a person who had been travelling to South Africa. This virus was further transmitted to three secondary schools and a concert with ca 2,000 participants in the same region, and subsequently to other events in other parts of Denmark. Furthermore, a hospital outbreak has been identified on a geriatric ward where at least four patients older than 80 years and two employees tested positive for Omicron. All of them were previously vaccinated. This study did not require ethical approval as it is based on routine surveillance data on COVID-19. Since the identification of the first Omicron case in Denmark, a steep increase in the number of cases has been observed. A major driver of this development was a large party with young adults -a population group with more social and close connections than adults and children. The rapid spread of the Omicron variant in Denmark is worrying, but not unexpected. On 2 December 2021, the European Centre for Disease Prevention and Control assessed a high probability of new introduction and community spread [10] . The response strategy in Denmark has been to delay transmission of the Omicron variant in order to gain time for roll-out of the third vaccine dose and the recently initiated vaccination programme for children aged 5 to 11 years. However, the rapid acceleration of cases catalysed by superspreading events challenged the mitigation. Despite the capacity to detect Omicron cases early, implementation of travel restrictions and implementation of extended contact tracing efforts, more than one in five cases cannot be linked to previous cases. This indicates that within 1.5 weeks from identifying the first case of Omicron, there is already widespread community transmission in Denmark, which challenges further epidemic control. It is of concern that 83% of cases occurred in fully or booster-vaccinated people. Whether this observation is an artefact as the major superspreading events and subsequent chains of transmission have occurred primarily in young adults, and not yet spread to children, who have not been vaccinated, is still too early to Table S1 ), rooted on the Wuhan-Hu1 strain (grey node). Introductions are denoted in the heatmap as 'Abroad' [7] [8] [9] . say. The data are too immature to allow analysing the cases by vaccine type. However, it can be mentioned that 76% of the Danish population are fully vaccinated and 84% of these have received Comirnaty (Pfizer/ BioNTech, Mainz, Germany/ New York, United States) followed by 14% with Spikevax (Moderna, Cambridge, United States). The composition of cases as well as the short follow-up period may affect the observed proportion of hospitalised cases. The proportion of asymptomatic cases should be interpreted considering the massive testing of contacts and relatively quick followup of cases, which means that SARS-CoV-2 Omicron cases may be caught early in their course of infection, potentially before the development of symptoms. Of note, the earliest Omicron cases in Denmark occurred before South Africa announced the emergence of this variant. These cases reported travel history from Qatar and the Netherlands, indicating that the variant might have spread from the African continent before this. Also, later Danish travel-related cases are not only found among travellers returning from South Africa but also from other European countries, indicating that community spread is likely to be more widespread than reported. Denmark has one of the highest RT-PCR testing capacities in the world and screens all positive RT-PCR tests with an Omicron-specific PCR [11] . Linking this information with national registers allows us to give a detailed overview of the early spread of the Omicron variant. We find several reasons for concern: (i) the rapid spread shortly after introduction despite extensive contact tracing efforts, (ii) the occurrence of several superspreading events with high attack rates and (iii) the high proportion of fully vaccinated Omicron cases. We observed nine hospitalisations and one case needed intensive care treatment. Owing to limited follow-up time and few admissions, it is too early to conclude on the severity of the Omicron variant. We show a rapid increase and spread of the SARS-CoV-2 Omicron variant in Denmark, a European country with high testing capacity, high vaccination coverage and limited natural immunity through SARS-CoV-2 infection. The introduction and spread occurred despite an early and comprehensive public health response. Spread was catalysed by superspreading events and challenges further epidemic control. Information from the earlier travel-related cases, with no travel history to Africa, suggests that community transmission is more widespread than reported. The high proportion of fully vaccinated Omicron cases is a concern, the implications are still being described. It is too early to draw conclusions on the severity of Omicron compared to previous SARS-CoV-2 variants, analyses on this are ongoing. Classification of Omicron (B.1.1.529): SARS-CoV-2 variant of concern Covid-19 -Danmark (kommunalt) Covid-19 -Danmark (regionalt) Genomic overview of SARS-CoV-2 in Denmark Rapid surveillance platforms for key SARS-CoV-2 mutations in Denmark Electronic reporting of diagnostic laboratory test results from all healthcare sectors is a cornerstone of national preparedness and control of COVID-19 MAFFT multiple sequence alignment software version 7: improvements in performance and usability IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences Threat assessment brief: Implications of the further emergence and spread of the SARS CoV 2 B.1.1.529 variant of concern (Omicron) for the EU/EEA first update South African Institute for Communicable Diseases (NICD) Division of the National Health Laboratory Service. New COVID-19 variant detected in South Africa Danmark indfører indrejserestriktioner for syv lande i det sydlige Afrika. [Denmark introduces entry restrictions for seven countries in southern Africa De to mistaenkte tilfaelde af coronavirus varianten Omicron i Danmark er nu bekraeftet Nye skaerpede retningslinjer for smitteopsporing ved smitte eller mistanke om smitte med den nye virusvariant Omicron. [New stricter guidelines for contact tracing in cases of infection or suspected infection with the new virus variant Omicron Danmark indfører skaerpede rejserestriktioner for yderligere tre lande i det sydlige Afrika [Denmark introduces stricter travel restrictions for another three countries in southern Africa Copenhagen: Sundhedsministeriet; 2021 Indrejsereglerne skaerpes yderligere med krav om test ved ankomst fra udvalgte lufthavne. [Entry rules further tightened with requirement to test on arrival from selected airports Status på omikron-varianten (B.1.1.529) pr. 05.12.21. [Status of the Omikron variant (B.1.1.529) on 5 Naere kontakters naere kontakter skal ikke laengere gå i selvisolation Copenhagen: Sundhedsstyrelsen; 2021 License, supplementary material and copyright We would like to thank the following people for their tireless commitment in response around Omicron, working close to non-stop including weekends and nights: Supplementary Table S1 acknowledges the contributors of GISAID genomes used in this study. None declared. LE, LM and TF drafted the manuscript with all authors contributing by editing and conducting the laboratory analyses, data collection, curation and analyses. All authors read and approved the final version of the manuscript.This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. 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