key: cord-0715749-skutw1z0
authors: Wang, Xiaoyang; Lin, Shengying; Tang, Roy Wai-Lun; Lee, Hung Chun; Chan, Ho-Hin; Choi, Sheyne S.A.; Leung, Ka Wing; Webb, Sarah E.; Miller, Andrew L.; Tsim, Karl Wah-Keung
title: Polygoni Multiflori Radix extracts inhibit SARS-CoV-2 pseudovirus entry in HEK293T cells and zebrafish larvae
date: 2022-05-09
journal: Phytomedicine
DOI: 10.1016/j.phymed.2022.154154
sha: 44393ca6f2a475e1a8889d8cf9bd0b66ceb7781e
doc_id: 715749
cord_uid: skutw1z0

BACKGROUND: Globally, COVID-19 has caused millions of deaths and led to unprecedented socioeconomic damage. There is therefore, in addition to vaccination, an urgent need to develop complementary effective treatments and/or protective and preventative therapies against this deadly disease. METHODS: Here, a multi-component testing platform was established to screen a library of herbal extracts from traditional Chinese medicine (TCM), to identify potent herbal extracts/phytochemicals as possible therapeutics for COVID-19. We utilized assays for spike protein (S-protein) binding to angiotensin-converting enzyme II (ACE2); the enzymatic inhibition of 3CL protease; and entry of the SARS-CoV-2 pseudovirus into cultured HEK293T cells and zebrafish larvae. RESULTS: Over a thousand herbal extracts were screened and approximately 20 positive hits were identified. Among these, we found that the water and ethanol extracts of Polygoni Multiflori Radix (PMR) significantly inhibited S-protein binding to ACE2, 3CL protease activity, and viral entry into the cell and fish models. The water extract was more effective than the ethanol extract, with IC(50) values of 25 to 500 µg/ml. In addition, the polysaccharide-depleted fraction of the former, and epigallocatechin gallate (EGCG) which was found in both extracts, displayed significant antiviral activity. CONCLUSIONS: Our results indicate that the water and ethanol extracts of PMR have an inhibitory effect on SARS-CoV-2 pseudovirus host-cell entry. Furthermore, EGCG might be an active component of PMR, which blocks SARS-CoV-2 entry to cells. Taken together, our findings suggest that PMR might be considered as a potential treatment for COVID-19.

As of December 2021, the COVID-19 pandemic (caused by variants of SARS-Cov-2 virus) has resulted in more than 270 million confirmed cases and caused over 5 million deaths since its outbreak in December 2019. In India, the largest number of daily confirmed cases has reached 400,000 (World Health Organization, https://covid19.who.int/, accessed on 15/12/2021). These figures clearly indicate the highly contagious nature of the virus, which has caused a public health emergency, as well as unprecedented damage to the global economy. Despite various COVID-19 vaccines having been approved for public use, which are effective in preventing the most life-threatening symptoms, 100% protection cannot be guaranteed especially with the emergence of new viral variants. In addition, in some cases the vaccines themselves have been reported to have a variety of side effects. As such, there is still an urgent need for the development of potent non-vaccine-based therapies against this deadly disease Mulligan et al., 2020) .

Since the start of the pandemic, much has been discovered about the route of entry and infection of SARS-CoV-2 virus. Both endosomal and non-endosomal pathways are reported to be involved in the viral entry mechanism, although the former is considered the main route.

During infection by SARS-CoV-2, the spike (S) protein of the virus recognises angiotensinconverting enzyme 2 (ACE2) on the host cell surface, and this triggers viral entry via endocytosis (Shang et al., 2020; Wang et al., 2020) . Once inside the host cells, nonstructural proteins, such as 3CL protease, enable viral replication and proliferation, leading to the widespread pathogenic damage of characteristic of this disease (Bristow et al., 2020) . In view of this entry and replication mechanism, several proteins (including ACE2, S-protein and 3CL protease) have been identified as potential drug targets for the prevention and/or treatment of COVID-19. As an anti-COVID-19 strategy, small molecules that bind one or more of these proteins, inhibit the route of SARS-CoV-2 entry into cells, and/or disrupt the downstream biological activities of the virus should be identified (Prasansuklab et al., 2021) .

In China, traditional Chinese medicine (TCM) has been reported to have good efficacy in the clinical treatment of viral infections. Indeed, in 2003, when SARS-CoV-1 began to threaten public health, several TCM products were identified to effectively relieve the symptoms, reduce the number of fatalities, and prevent the course of the disease (Yang et al., 2020) . Today in China, more than ten prescriptions containing TCM have been recommended by clinics for the treatment of mild and moderate cases of COVID-19. Their promising efficacy has inspired the search for other novel TCMs as potential treatments for this disease .

Here, we established a screening platform for anti-SARS-CoV-2 infection by testing the ability of an extensive library of TCM herbs/single molecules to inhibit the S-protein-ACE2 interaction, 3CL protease activity, and/or viral entry into cells and zebrafish (Danio rerio) larvae. From the 1,000 herbal extracts screened, >20 positive hits were identified. Among these, the water and ethanol extracts of Polygoni Multiflori Radix (PMR) yielded significant inhibition of SARS-CoV-2 host-cell entry. PMR is a well-established TCM, which has previously been utilized for the treatment of various diseases, including cancer, liver cirrhosis and hair loss. The major phytochemicals in PMR are stilbenes, quinones, and flavonoids (Ho et al., 2007; Lee and Lee, 2015) . Here, we demonstrate that the water and ethanol extracts of PMR are non-toxic, and they significantly inhibit the S-protein/ACE2 interaction, 3CL protease activity, and viral entry into both HEK293T cells and zebrafish larvae. As such, they are strong contenders for a more in-depth analysis prior to utilizing the live virus in mammalian models, with a view for potential drug development and/or clinical application.

HEK293T cells (American Type Culture Collection, Manassas, VA, USA) were maintained in high glucose Dulbecco's Modified Eagle Medium (DMEM) supplemented with 1% penicillin/streptomycin and 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA; herein called culture medium) at 37°C in an incubator with water-saturated atmosphere and 5% CO 2 . Fresh culture medium was supplied every other day. HEK293T cells overexpressing hACE2 (human ACE2) were prepared by transfection with the pcDNA3.1-hACE2 plasmid (Addgene, Watertown, MA, USA). The cell viability was determined as described previously (Wu et al., 2019) .

PMR powder (10.0 g), grounded from roots of Polygonum multiflorum Thunb., was placed in a 250-ml round-bottomed flask and dissolved in 100 ml 90% ethanol or distilled water. The solutions were refluxed for 1 h before being filtered through a paper filter (Advantec, Tokyo, Japan). These were then evaporated to dryness with a rotary evaporator to yield final ethanol (PMR EtOH ) and water (PMR water ) extracts of 2.05 g and 4.16 g, respectively. To fractionate the PMR water , 0.64 g of this extract was dissolved in 10 ml water and then added to 40 ml EtOH. The mixture was stored at 4°C for 16 h, then centrifuged at 7,000 rpm for 15 min and filtered through a paper filter (Advantec). The precipitate was washed twice with 20 ml water and dried to yield a polysaccharide-enriched fraction (PS enrich ) of ~0.32 g, whereas the filtrate was evaporated to dryness with a rotary evaporator to yield a polysaccharide-depleted fraction (PS deplete ) of ~0.23 g.

In accordance with a well-established methodology of colourimetry (HKCMMS; Hong Kong Chinese Medicine Medica Standards, 2008), PS deplete or PS enrich (40 mg) was dissolved in 50 ml boiling water in a 50-ml volumetric flask. Three ml of this solution was added to another 50-ml volumetric flask and diluted 10 times. Two ml of this solution was then added to a 10-ml tube and 6 ml of anthrone sulphuric acid solution was added. The mixture was cooled on ice for 15 min and then the absorbance was measured at 625 nm.

HPLC detection and calculation were conducted according to methods developed by the HKCMMS utilising PMR EtOH (1 mg/ml), PMR water (1 mg/ml), PS enrich (1 mg/ml), PS deplete (1 mg/ml), 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG, 0.1 mg/ml), emodin (0.1 mg/ml), physcion (0.1 mg/ml) and EGCG (0.1 mg/ml) (all at purity >95% from Chengdu Must, Chengdu, China). The HPLC gradient comprised 8% acetonitrile for 0-5 min, 8-20% acetonitrile for 5-15 min, 20% acetonitrile for 15-35 min, and 20-30% acetonitrile for 35-45 min. The detection wavelength was 230 nm.

HEK293T cells at 80% confluence were transfected with components of SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike-Pseudotyped Lentiviral Kit (NR-52948; BEI Resources, NIAID, Bethesda, MD, USA), including SARS-CoV-2 Spike Glycoprotein (NR-52514), lentiviral backbone expressing Luciferase and ZsGreen (NR-52516), and helper plasmids (NR-52517, NR-52518, NR-52519) using Lipofectamine™ 3000 (Thermo Fisher Scientific) or JetPRIME (Polyplus, Shangai, China) transfection reagent, according to the manufacturers' instructions. After 72 h, the SARS-CoV-2 pseudotyped-virus particles (hereafter called pseudovirus) were collected and passed through a 0.45 µm filter. In most HEK293T cellbased experiments, the pseudovirus was used directly. For the zebrafish experiments, the pseudovirus was subsequently purified using polyethylene glycol (PEG; hereafter called PEG-pseudovirus; Lo and Yee, 2007) , and the culture medium was replaced with phosphate buffered saline (PBS; 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 pH 7.4).

The pseudovirus and PEG-pseudovirus were stored at -80°C until required.

ACE2-overexpressing HEK293T cells were seeded into 48-well plates, after which 400 µl culture medium containing SARS-CoV-2 pseudovirus (100 µl) and PMR EtOH or PMR water (at various concentrations) were added, and they were incubated at 37°C for 24 h. This medium was then replaced with fresh medium, and the cells allowed to recover for 48 h. The cells were washed with PBS just before the luciferase assay. PMR EtOH , PMR water PS enrich and PS deplete were tested at final concentrations of 1, 10, 25, 50, and 100 µg/ml, whereas EGCG was tested at 10, 25, 50, 75 and 100 µM. An anti-SARS-CoV-2 neutralizing antibody (at 1 µg/ml; A19215, ABClonal, Woburn, MA, USA) was used as a positive control, whereas a solvent blank without the pseudovirus was used as a negative control. The inhibition percentage was determined according to the luciferase activity normalized to the luciferase activity without PMR treatment.

The luciferase assay was conducted as previously described (Wu et al., 2019) . The percentage inhibition of each sample was calculated as follows: Inhibition rate = (Luciferase activity of the solvent blank − Luciferase activity of the sample) / (Luciferase activity of the solvent blank − Luciferase activity of group without pseudovirus) × 100%.

Spike protein inhibition was analysed with SARS-CoV-2 Spike-ACE2 Binding Assay kit (ImmunoDiagnostics Ltd. Hong Kong, China) according to the manufacturer's instructions.

The reaction was terminated by adding 2 M H 2 SO 4 , and a FlexStation 3 microplate reader (Molecular Devices, San Jose, CA, USA) was used to quantify the data. The percentage of inhibition was calculated as follows: Percentage of inhibition = (P Avg − S Avg ) / P Avg × 100%, where P Avg and S Avg are the mean OD values of the positive control and test sample, respectively.

Extracts were tested for binding 3CL protease on a fluorogenic substrate with the SensoLyte SARS-CoV-2 3CL Protease Activity assay kit (AnaSpec, San Jose). The 3CL

protease bound with the substrate emitted fluorescence at 460 nm when using a 360 nm excitation wavelength. The percentage of Inhibition was calculated as follows: Percentage of inhibition = (P Avg , b − S Avg , b) / P Avg , b × 100%, where P Avg , b and S Avg , b are the mean fluorescence of the positive control and test sample, respectively, subtracted from the mean fluorescence of the blank.

Zebrafish larvae at 3 days post-fertilization (dpf) were treated for 6 h with Danieau's solution (Westerfield, 2000) ± 125 µg/ml PMR water , or with Danieau's solution containing 0.1% DMSO ± 30 µg/ml PMR EtOH in a 24-well plate (SPL Life Sciences, Gyeonggi-do, Korea). Each experiment was conducted using 10 embryos per treatment group, and repeated n = 4. The larvae were then transferred to 96-well plates, one larva per well, containing the respective treatment solution plus 8 µl PEG-pseudovirus, incubated at 28°C for 72 h, until they reached ~6.25 dpf. The larvae from each treatment group were pooled and washed with Milli-Q water for 6 × 20 min with gentle agitation prior to RNA extraction.

RNA extraction and reverse transcription were conducted as described (Kelu et al., 2017) . The converted cDNA was then amplified, for 35 cycles, by PCR using 2 × Rapid Taq Master Mix (Vazyme Biotech, Nanjing, China), after which the PCR products were separated on a 2% agarose gel. The band intensities were quantified using ImageJ (NIH; https://imagej.nih.gov/ij/). The level of expression of luciferase (Luc) mRNA was determined against that of glucose-6-phosphate dehydrogenase (g6pd) mRNA, and the expression levels following treatment with PMR water or PMR EtOH were measured. The primers used were as 

The chemical structures of phytochemicals were downloaded from Pubchem (https://pubchem.ncbi.nlm.nih.gov/), and the S-protein structure was downloaded from the Protein Data Bank (https://www.rcsb.org/). Virtual screening was performed with SEESAR (Version 11.0, https://www.biosolveit.de/) as follows: (i) The binding site was determined according to the residues forming the identified druggable pocket. Ligand binding states including protonation and tautomeric forms were subsequently evaluated using the ProToss method to generate the most accessible hydrogen network. (ii) Docking modulation was performed using the "Compute LeadIT Docking" mode in the FlexX algorithm; ten binding conformations for each ligand were generated. (iii) The binding energy (i.e., ∆G) and estimated HYDE affinity (KiHYDE) for each ligand pose were calculated using the "Assess Affinity with HYDE in SEESAR" mode in the HYDE rescoring function (Spagnolli et al., 2021) .

In our HPLC analysis, THSG, emodin, physcion and EGCG were utilised as standard markers of the PMR extracts (Fig. 1) . PMR water consisted of 5.5% THSG, 0.03% emodin, 0.02% physcion and 0.71% EGCG, whereas PMR EtOH contained 1.86% THSG, 0.80% emodin, 0.58% physcion and 0.37% EGCG. The extractive efficacy was 41.6% for PMR water and 20.5% for PMR EtOH. These parameters served as quality controls for subsequent experiments.

An ELISA was utilised to test the binding of S-protein to ACE2. Initially, a standard inhibitor (calibrated to NIBSC code 20/136), provided by the supplier, was tested as a positive control ( Fig. 2A) . The inhibitor blocked binding to S-protein in a dose-dependent manner. To investigate the binding activity between the PMR extracts and S-protein, PMR water or PMR EtOH were tested at various concentrations up to 2.5 mg/ml. Both extracts inhibited the binding in a dose-dependent manner (Fig. 2B) , with an estimated IC 50 of ~1 to 2 mg/ml. We also tested the effects of PMR extracts on enzymatic activity of 3CL protease. We used GC-376 as a positive control, which has a broad-spectrum of anti-viral effects . As shown in Fig. 3 , PMR water and PMR EtOH inhibited 3CL protease activity in a dosedependent manner, with IC 50 values of ~0.25 mg/ml and ~0.5 mg/ml, respectively.

Interestingly, PMR water displayed a higher maximal inhibition rate than PMR EtOH , i.e., ~100% versus ~70% inhibition.

As the S-protein and 3CL protease results indicated that the PMR extracts might prevent viral entry into host cells, we investigated the effect of PMR water and PMR EtOH on the entry of a Luc-expressing SARS-Cov-2 pseudovirus into ACE2-overexpressing HEK293T cells. The luciferase-generated luminescence was quantified to determine the amount of virus entering the cells. The luminescence decreased as the pseudovirus was diluted, indicating the efficacy of the assay (Fig. 4A ). An anti-SARS-CoV-2 neutralising antibody (positive control) inhibited total pseudoviral entry by ~75%. Furthermore, PMR water or PMR EtOH inhibited pseudoviral entry in a dose-dependent manner (Fig. 4B) . However, maximal inhibition was only achieved by PMR water , which displayed ~3-fold higher effectiveness than PMR EtOH .

Neither extract affected the cell viability up to 100µg/ml (Supplementary Fig. S1 ).

The expression of the Luc gene was used as an indicator of successful PEGpseudovirus entry in zebrafish larvae. The 3-day-old larvae were pre-treated with PMR water or PMR EtOH for 6 h before being treated with either of these extracts plus PEG-pseudovirus for a further 72 h. Both extracts significantly inhibited the expression of Luc when compared with the respective untreated and DMSO controls (Fig. 5A&B) . These results suggest that both extracts can prevent pseudovirus entry into zebrafish larvae.

Due to its higher potency, PMR water was selected for further fractionation. Thus, it was subjected to EtOH precipitation to obtain PS enrich and PS deplete . We found that most of our selected marker chemicals from PMR water remained in PS deplete (Fig. 6A) , and that PS enrich contained >86% carbohydrate, of which >75% were polysaccharides with molecular weights >10 kDa (Fig. 6B) . In the pseudoviral entry assay, PS deplete was more potent than PMR water in blocking viral entry, with an IC 50 value of ~15 µg/ml (Fig. 7A) . Indeed, maximal blocking was revealed by PS deplete alone.

The enriched chemicals within PS deplete were also tested using the viral entry assays.

Several phytochemicals (e.g., THSG, emodin and EGCG) were enriched in PMR water (Sun et al., 2018; Yi et al., 2007) . Of these, EGCG inhibited pseudovirus entry robustly in a dosedependent manner, exhibiting complete inhibition at ~100 M and an IC 50 at ~30 M (Fig.   7B ). As PMR water contained higher amounts of EGCG than PMR EtOH (0.71% vs 0.37%), this might account for the different levels of performance of these extracts in the antiviral assays.

Compared with EGCG, the other PMR phytochemicals tested, exhibited no significant effects on viral entry (Supplementary Fig. S2) . To further validate our hypothesis, the receptor binding domain (RBD) of the S-protein was selected as a binding site for docking analysis.

The RBD inhibitor K22 (Xiu et al., 2020) , was predicted to bind RBD with a binding energy of -12 KJ/mol. In comparison, EGCG was found to bind the RBD with an energy of -7.5 KJ/mol, whereas no significant binding activity was observed for the other PMR chemicals (Supplementary Fig. S3A) . As testing platforms based on the omicron variant of SARS-CoV-2 were not available to our research team, the effect of EGCG on this variant was evaluated by computational docking (Supplementary Fig. S3B) . Interestingly, EGCG was predicted to require less energy to bind to this variant than to the wildtype virus (i.e., -15.2 vs -7.5 KJ/mol). This suggests that EGCG, and its parental TCM herb, PMR, might also have potent antiviral effects with regards to the omicron variant of SARS-CoV-2.

Given that COVID-19 has caused (and is still causing) so many deaths globally, there is an urgent need to find novel treatments that are protective against and/or that can treat the symptoms of SARS-CoV-2 infection. TCM is a rich source of herbs/phytochemicals that can Huoxiang Zhengqi (containing 10 TCM herbs including Atractylodis Rhizoma, Citri Reticulata Pericarpium, Magnoliae Officinalis Cortex, and Angelicae Dahuricae Radix), is also being used to treat this disease (Al-Romaima et al., 2020; Luo et al., 2020) . Thus, other TCMs might also prove to be promising therapies for the prevention and treatment of COVID-19.

Polygonum plants are a family of herbs that have good anti-viral properties (Sun et al., 2016) . Taking P. perfoliatum L. as an example, the herbal extract and its corresponding chemical, quercetin-3-O-β-D-glucuronide, has been reported (through in vivo studies) to inhibit influenza A virus (Fan et al., 2011) . Our new results also demonstrate the antiviral properties of Polygonum-type TCMs. Like other members of the Polygonum family, PMR has long been utilised in medicinal practices against various diseases as it has a good record of safety and effectiveness (Sun et al., 2016) . Although the antiviral properties of PMR have not been reported before, the antiviral activity of EGCG against SARS-CoV-2 was reported by . We confirmed the antiviral effects of EGCG in our testing platforms, and suggest that this is one of the active components in our PMR extracts. In addition, as EGCG docked with the S-protein of the omicron SARS-CoV-2 variant with higher binding energy than with the wildtype virus, this suggests that PMR extracts might be highly effective at protecting against omicron infection. However, as the amount of EGCG was relatively low in the PMR extracts (e.g., ~0.71% in PMR water ), the other phytochemicals might also have antiviral properties.

In conclusion, COVID-19 remains a disease of major concern throughout the world and effective treatments are still urgently required to complement vaccination. Here, we established a dual screening platform comprising various in vitro and in vivo experimental approaches, to provide a first step in the identification of TCMs with anti-COVID-19 potential.

Using this platform, we established that the water and ethanol extracts of PMR were nontoxic and inhibited the S-protein-ACE2 interaction, the activity of 3CL protease, and entry of the SARS-CoV-2 pseudovirus into HEK293T cells and zebrafish larvae. We also found that 

All the procedures used with zebrafish in this study were conducted in accordance with the guidelines and regulations outlined by the Animal Ethics Committee of HKUST, and the Department of Health, Hong Kong.

The authors declare that there is no conflict of interest associated with this publication. inhibited by PMR water and PMR EtOH in a dose-dependent manner. The inhibition percentage was determined from the binding signal normalized to the interaction between spike RBD and hACE2 without extract. The data represent mean ± SD (n = 3). Fig. 3 . Inhibition of 3CL protease activity. PMR water and PMR EtOH inhibited the activity of 3CL protease of SARS-CoV-2 in a dose-dependent manner. A control inhibitor, GC-376 (500 M), was used as a positive control. The inhibition percentage was determined according to the optical density normalized to the 3CL protease activity without extract. The data represent mean ± SD (n=3).

The amount of intracellular luciferase activity (indicating the rate of virus entry) was measured at different dilutions of the pseudovirus. (B) Pseudovirus entry was inhibited by PMR water or PMR EtOH in a dose-dependent manner. The inhibition percentage was determined according to the luciferase activity normalized to the luciferase activity without extract. A SARS-CoV-2 neutralizing antibody was used as a positive control. The data represent mean ± SD, (n = 3).

zebrafish. Larvae at 3 dpf were pre-treated with (A) PMR water or (B) PMR EtOH , then co-treated with the respective extract and SARS-CoV-2 PEG-pseudovirus for a further 72 h. PCR amplification of whole larval cDNA showed the relative level of Luc expression in the treatment and control groups (left). The relative level of Luc expression was quantified by normalization to the expression level of g6pd (right). The data show that treatment with PMR water or PMR EtOH inhibited the expression of Luc (and thus the entry of PEG-pseudovirus) in larvae. The data represent the mean ± SD (n=4), and statistical differences were tested using the 2-sample t-test, ****p<0.0001. mg/ml, were revealed at 230 ± 4 nm. EGCG (1), THSG (2), emodin (3) and physcion (4) are marked. (B) PS deplete and PS enrich (0.5 mL each at 1 mg/mL dried weight) were transferred to a 10 kDa cut-off concentrator (Sartorius, German) and centrifuged at 14,000 g for 15 min.

The carbohydrate content was measured with phenol-sulfuric acid. The values are expressed as mean ± SD, n = 3. EGCG, all in a dose-dependent manner. The data represent mean ± SD, (n = 3). A SARS-CoV-2 neutralizing antibody was used as the positive control.

Advances in the treatment of novel coronavirus disease (COVID-19) with Western medicine and traditional Chinese medicine: a narrative review

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Polygoni Multiflori Radix, 2nd ed. Department of Health, The Government of Hong Kong Special Administrative Region

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Reflections on treatment of COVID-19 with traditional Chinese medicine

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Anti-COVID-19 drug candidates: a review on potential biological activities of natural products in the management of new coronavirus infection

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Comprehensive analysis of polygoni multiflori radix of different geographical origins using ultra-highperformance liquid chromatography fingerprints and multivariate chemometric methods

Efficacy and safety of traditional Chinese medicine in coronavirus disease 2019 (COVID-19): a systematic review and meta-analysis

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The Zebrafish Book. A guide for the laboratory use of zebrafish (Danio rerio)

Isoorientin derived from Gentiana veitchiorum Hemsl. flowers inhibits melanogenesis by down-regulating MITF-induced tyrosinase expression

Inhibitors of SARS-CoV-2 entry: current and future opportunities

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Identification and determination of the major constituents in traditional Chinese medicinal plant Polygonum multiflorum Thunb by HPLC coupled with PAD and ESI/MS

 11 We thank Prof. Jonathan S. Marchant (Medical College of Wisconsin, USA) for providing us with the SARS-related Coronavirus 2 lentiviral kit and ACE2 plasmid.