key: cord-0715444-w5ddc5xo authors: Laumbach, Robert J.; Mainelis, Gediminas; Black, Kathleen G.; Myers, Nirmala T.; Ohman-Strickland, Pamela; Alimokhtari, Shahnaz; Hastings, Shirin; Legard, Alicia; de Resende, Adriana; Calderón, Leonardo; Lu, Frederic T.; Kipen, Howard M. title: Presence of SARS-CoV-2 Aerosol in Residences of Adults with COVID-19 date: 2022-02-01 journal: Ann. Am. Thorac. Soc. (Online) DOI: 10.1513/annalsats.202107-847rl sha: ac8d3dada8d55427916e02b0ba046bd8f1f6ee01 doc_id: 715444 cord_uid: w5ddc5xo nan public health responses to vaccine-resistant variants or future novel respiratory viruses. Reducing attack rates in households, estimated to be as high as 54% in the United States, is a key strategy (1) . In addition to close physical contact, emerging opinion suggests that airborne transmission is linked to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread, particularly in lower-socioeconomic-status households with greater crowding, even if isolation and personal protective equipment minimize large particle transmission (2) (3) (4) (5) . The size-dependent airborne behavior of particles originating from the respiratory tract has a continuous distribution from tens of nanometers to tens of microns. Recognizing this continuity, there are two primary pathways, requiring different control strategies, by which respiratory viral infections spread through air to others. First, larger respiratory droplets that rapidly settle onto surfaces, typically within 1-2 meters of the source, are amenable to hand hygiene, social distancing, and face masks. Second, albeit with more limited direct evidence, is aerosolization and spread of smaller respiratory droplets, or droplet nuclei, primarily ,0.5 micrometers in final size, capable of staying suspended in air for hours and requiring filtering or ventilation for interdiction (2) (3) (4) . We report the first naturalistic observations of household air contamination with SARS-CoV-2 RNA. We know of no prior reports of air sampling for SARS-CoV-2 RNA in homes without manipulation of the behavior or activity of participants. Rutgers Institutional Review Board approved this study, and participants provided informed consent. Recruitment occurred in fall and winter of 2020-2021 through an e-mail flyer at the time of notification of test positivity. Adults testing positive within the prior 7 days were eligible to participate. Saliva screening at the first home visit verified continued positivity (Table 1) . Air samples were collected for 24 hours on polytetrafluoroethylene (PTFE) filters (SKC Inc.) in two separate rooms (if available) in each participant's home using an openface filter holder and Leland Legacy pump (SKC Inc.) operated at 10 L/min. Samples were eluted in RNA-grade water and analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) for the presence of three SARS-CoV-2-specific genes. There is no universal protocol for RT-PCR testing of SARS-CoV-2, let alone for its analysis in environmental samples (6) . Our selected laboratory (Infinite BiologiX) used a U.S. Food and Drug Administration-approved procedure developed at Rutgers to target three genomic regions of SARS-CoV-2: nucleocapsid (N) gene, spike (S) gene, and open reading frame-AB (ORF1-AB) region. To maximize detection sensitivity, we assessed presence (cycle threshold [CT] , 37) or absence of each gene in our air samples (7) . The selected rooms were defined as the isolation room (the room used primarily, but not exclusively, by the subject) and the common room (a separate but adjacent room). Participants recorded hours spent in both rooms during Letters 339 sampling, but instructions for self-isolation were not provided. Samplers were placed 1 meter away from the nearest wall and away from vents, windows, traffic flow, and obstructing furniture where possible. Samplers faced downward to avoid large droplets. The study included 11 homes (Table 1) with 20 air samples (60 individual SARS-CoV-2 gene RT-PCR tests) collected from 11 isolation rooms and 9 common rooms ( Table 2) . In addition to the primary case, one or more known or suspected recently positive individuals were reported to be present in 4 of 11 (36%) homes at the time of sampling. During sampling, participants reported spending between 10 and 24 hours in the isolation room. Seventy-three percent of participants reported spending some time in the common room (range 0-14 h) and 45% of participants reported time in other areas of the home (range 0-8 h). For each of the three genes, the percentage of homes with a positive air sample ranged from 36% to 45% in the isolation room and from 22% to 67% in the common room. Eight homes out of 11 (73%) had at least one gene detected, and 5 of 11 isolation room samples had at least two genes detected. Six of nine homes with sampling in both the isolation room and common room had at least one gene detected in the common room (Table 2) , and four of these common rooms had two genes detected. Seven of these nine homes reported no other cases in the household (Table 1) , including the two living alone, and in five of these homes, the common room was positive for viral aerosols. An additional occupant who recently tested positive or had symptoms consistent with COVID-19 was present in only two of seven (29%) homes with multiple occupants and a valid common room test. Our results provide strong empirical support that aerosols of small respiratory droplets and nuclei containing airborne SARS-CoV-2 RNA are present both within and outside of home isolation rooms, presenting infection risk beyond close contact with other occupants. Our indoor air sampling data clearly demonstrate that measurable airborne SARS-CoV-2 RNA is present in home air of most infected individuals. We found SARS-CoV-2 viral RNA, likely as both free virus and bound to other particulate matter (PM), not only in the isolation room but, importantly, elsewhere in the home (common room), consistent with high risk of home airborne transmission. Previously, detection of airborne SARS-CoV-2, likely as part of PM, has been limited to the hospital or clinic setting (8) (9) (10) (11) (12) , an automobile cabin (13) , and two reports identifying it in outdoor PM samples (14, 15) . Further buttressing our findings is a study of viral aerosols measured only in isolation rooms of apartments at a specified distance of 2 meters from the participant, using a 20-minute scripted (nonnaturalistic) air sampling protocol (16) . Our novel empirical findings support the hypothesis that exposure to airborne small droplets and/or droplet nuclei is a pathway for COVID-19 transmission and a candidate explanation for high household attack rates (1) . Despite models, laboratory experiments, and theory-based discussions, previous field data have not empirically addressed or clarified the relative importance of real-world exposure pathways that must be interdicted to prevent transmission of COVID-19. Studies are needed with adequate power and definitive assessment of infection status of all household members, their locations within the household, clear discrimination between aerosols and larger droplets by size-selective sampling, and assessment of aerosolized virus viability (12) . Author disclosures are available with the text of this letter at www.atsjournals.org. Household transmission of severe acute respiratory syndrome coronavirus-2 in the United States Airborne transmission of SARS-CoV-2: the world should face the reality Identifying airborne transmission as the dominant route for the spread of COVID-19 The coronavirus pandemic and aerosols: does COVID-19 transmit via expiratory particles? 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Are the portable air cleaners (PAC) really effective to terminate airborne SARS-CoV-2? Acknowledgment: The authors thank their referring physicians and Vault Health for participant referrals. They also thank Infinity BiologiX and the late Andrew Brooks, Ph.D., for their invaluable assistance as well as the participants for allowing the authors into their homes.