key: cord-0713243-twdgoe6h
authors: Kumar, Manish; Dhangar, Kiran; Thakur, Alok Kumar; Ram, Bhagwana; Chaminda, Tushara; Sharma, Pradeep; Kumar, Abhay; Raval, Nirav; Srivastava, Vaibhab; Rinklebe, Jörg; Kuroda, Keisuke; Sonne, Christian; Barcelo, Damia
title: Antidrug Resistance in the Indian Ambient Waters of Ahmedabad during the COVID-19 Pandemic
date: 2021-05-24
journal: J Hazard Mater
DOI: 10.1016/j.jhazmat.2021.126125
sha: 97f6f4d38f76fdc79812bc9120baaf099c461d22
doc_id: 713243
cord_uid: twdgoe6h

The ongoing COVID-19 pandemic increases the consumption of antimicrobial substances (ABS) due to the unavailability of approved vaccine(s). To assess the effect of imprudent consumption of ABS during the COVID-19 pandemic, we compare the 2020 prevalence of antidrug resistance (ADR) of Escherichia coli (E. coli) with a similar survey carried out in 2018 in Ahmedabad, India using SARS-CoV-2 gene detection as a marker of ABS usage. We found a significant ADR increase for in 2020 compared to 2018 in ambient water bodies, harbouring a higher incidence of ADR E.Coli towards non-fluoroquinolone drugs. Effective SARS-CoV-2 genome copies were found to be associated with the ADR prevalence. The prevalence of ADR depends on the efficiency of WWTPs (Wastewater Treatment Plants) and the catchment area in its vicinity. In year 2018 study, prevalence of ADR was discretely distributed, and the maximum ADR prevalence recorded was ~60%; against the current homogenous ADR increase, and up to 85% of maximum ADR among the incubated E.coli isolated from the river (Sabarmati) and lake (Chandola and Kankaria) samples. Furthermore, wastewater treatment plants showed less increase in comparison to the ambient waters, which eventually imply that although SARS-CoV-2 genes and faecal pollution may be diluted in the ambient waters, as indicated by low C(t)-value and E.coli count, the danger of related aftermath like ADR increase cannot be nullified. Also, Non-fluoroquinolone drugs exhibited overall more resistance than the quinolone drugs. Overall, this is probably the first ever study that traces the COVID-19 pandemic imprints on the prevalence of antidrug resistance (ADR) through wastewater surveillance and hints at monitoring escalation of other environmental health parameters. This study will make public and policyholders concerned about the optimum use of antibiotics during any kind of treatment.

The exponential rise in the consumption of antimicrobials in various applications such as medical, veterinary, domestic and agricultural and their leak to aquatic ecosystems has caused the global prevalence of antidrug resistance (ADR), which is being considered a major threat to public health (Rodriguez-Mozaz et al., 2015; Chatterjee et al., 2010; Baker-Austin et al., 2006) . The ADR is not only limited to the survival and infection by any particular type of microorganism, but can lead to life threatening diseases for both animals and human (Singer et al., 2008; Ferreira Da Silva et al., 2007; Jiang et al., 2013) . Due to lack of regulations on the prescription and non-prescription use of antimicrobials, its consumption rate in for example India has been increased by 105% from 2000 to 2015 while worldwide it is J o u r n a l P r e -p r o o f estimated to increase by 63% during 2010 to 2030 (Klein et al., 2018; GARP, 2011; Van Boeckel et al., 2015) . On the top of that, the rate of consumption of certain antimicrobials has escalated during the COVID-19 pandemic in an effort to minimize the risk of severe infections and mortality (Miranda et al., 2020; Liu et al., 2020) . Around 70% of patients have received antimicrobial treatment along with overuse of various antibiotics despite only 10% on average show microbial infections (Hsu, 2020; Rawson et al., 2020) . As most of the consumed drugs and their metabolites are excreted through urine and faeces, their discharge to aquatic environments depends on the removal efficiency of the WWTPs (Singer et al., 2008; Azuma et al., 2012; Takanami et al., 2010; Auerbach et al., 2007; Kumar et al., 2020a) . If the WWTP clearing rate is low, microorganisms exposed to antimicrobials and metabolites develops mutations causing ADR (Aali et al. 2014 , Alexander et al. 2020 , Guo et al. 2018 , Kumar et al., 2020a Kumar et al., 2020c) Thus, the increased use of antimicrobials in the current pandemic will probably pose an increased risk in terms of ADR during post COVID-19 as concerned by a number of recent studies (Kuroda et al., 2021; Lucien et al., 2021; Hsu, 2020; Kumar et al., 2020a; Asaduzzaman et al., 2020) .

The high consumption of antimicrobials causes an increase in the prevalence of ADR in several environmental compartments including drinking, waste and groundwater, sludge, sediments and municipal solid waste leachate (Al-Judaibi, 2014; Ferreira Da Silva et al., 2007; Kumar et al., 2020d; 2020e; Ram and Kumar, 2020; Zhang et al., 2015; Storteboom et al., 2010; Threedeach et al., 2012) . In case of for example E.coli isolates from the effluent of WWTPs have shown a higher prevalence of antidrug resistance as compared to the influent, which is probably due to poor treatment conditions, prolonged microbial activities, and chemical properties of the antimicrobial drugs (Reinthaler et al. 2003; Silva et al., 2006; Miranda and Castillo, 1998; Marcinek et al., 1998) . Specifically, the conventional treatment J o u r n a l P r e -p r o o f processes at WWTPs do not completely mineralise the parent antimicrobial drugs, and generate some residues, metabolites or transformation products that may have same biological activity as the parent drugs (Zhang et al., 2015; Kumar et al., 2020c) . Thus, WWTPs are considered hotspots for the spreading ADR due to high microbial density, horizontal gene transfer (HGT), nutritional richness and the availability of antimicrobial metabolites (Zhang et al., 2015; Threedeach et al., 2012; Silva et al., 2006) . Previous studies have reported a correlation between the prevalence of ADR and inefficiently treated wastewater discharge, having abundance of Escherichia coli (E. coli), extravasating to river and lake waters (Na et al., 2018; Yang et al., 2016; Honda et al., 2016; Biswas et al., 2015; Akhter et al., 2014; Ram and Kumar, 2020; Kumar et al., 2020d; 2020e) . Thus, a better understanding of the occurrence, distribution and frequency of antidrug resistance in the urban waters is needed to prevent or slower the rate of increase in ADR.

With the same purpose, presumptive actions are needed to study the prevalence of the ADR during wastewater treatment and the water bodies receiving the WWTP effluents. Wastewater based epidemiology (WBE) is an efficient way to trace the prevalence of ADR in highly COVID -19 infected areas, which are potentially major zones of high consumption of drugs, can be identified with the help of WBE approach for tracing the SARS-CoV-2 genome concentration in wastewaters (Kumar et al., 2020b) . Also, with the help of authorised software and apps (for example: Arogya-Setu app in India), the infected population within a certain region can be predicted. Identifying the WWTPs in such infected areas aids in correlating ADR with the elevated cases of COVID-19. Therefore, the impact of such highly contaminated zones on the prevalence of ADR in wastewaters need to be studied well.

J o u r n a l P r e -p r o o f ADR is not included in the water quality standards and guidance of India mostly due to the lack of proper treatment facilities in many cities where domestic wastewater is directly discharged to aquatic environments (IS 10500: 2012) . In this study, we select Ahmedabad City of Gujarat Province in western India with population of 5.6 million (2011 Census), to assess the prevalence of ADR in WWTP, lake and river locations within various zones of the city. The specific objectives of the present study are: i) to compare and discuss the prevalence of E. coli in the surface water and wastewater in Ahmedabad in order to have a prior knowledge of ADR pervasiveness in different compartments, ii) to analyse comparative status of the antidrug resistance in the E. coli isolated from the urban waters of the city and iii) to further understand the imprints of COVID-19 situation on the status of SARS-CoV-2 genome concentration and ADR prevalence at various zones of the city.

The water samples were collected from 6 different locations of Ahmedabad city on 23 rd June 2018, and 16 th October 2020 (Fig. 1) . Two locations on stretch of Sabarmati river: Nehru Bridge (NB) and Sardar Bridge (SB); two lakes: Kankariya Lake (KL) and Chandola Lake (CL), and two WWTP locations: Chandkheda (inlet: CI and outlet: CO) and Vasna, also known as Juhapura (inlet: VI and outlet: VO), selected to assess ADR. For SARS-CoV-2 gene detection, a total of 10 locations were selected to represent various zones of the city that comprises all ADR sampling locations. We kept ADR locations low to match the number of locations tested in 2018 (Ram and Kumar, 2020) . The geographical details about the selected locations are well described in our previous study by Ram and Kumar, (2020) HI9828 was used. The procedure for testing the isolation of E. coli for ADR is likewise described in Ram and Kumar, (2020) 

The SARS-CoV-2 RNAs were isolated and detected from 30 mL wastewater samples that were centrifuged at 4000×g for 40 minutes, followed by filtration of supernatant using 0.22micron syringe filter (Mixed cellulose esters syringe filter, Himedia). After filtration, 25 mL of the supernatant was treated with polyethylene glycol and NaCl at 80 g/L and 17.5 g/L respectively, and incubated at 17°C, 100 rpm overnight. The mixture was centrifuged for 90 min at 14000×g and the supernatant were discarded to collect a pellet containing viruses and their fragmented genes. The pellet was re-suspended in 300µL RNase-free water and kept in 1.5ml vials at -40 °C, until further analyses.

RNA isolation from the pellet with the concentrated virus was performed using NucleoSpin® RNA Virus isolation kit (Macherey-Nagel GmbH & Co. KG, Germany). The samples were spiked with MS2 phage as an internal control prior to the RNA extraction provided by TaqPathTM Covid-19 RT-PCR Kit. The nucleic acid was extracted and a Qubit 4 Fluorometer (Invitrogen) was used for RNA concentrations estimation. The molecular J o u r n a l P r e -p r o o f process inhibition control was evaluated through the MS2 phage for QA/QC analyses of nucleic acid extraction and PCR inhibition (Haramoto et al., 2018) . We have described the methodologies in Kumar et al., (2021; 2020b) .

Briefly, steps were carried out as per the guideline provided with the product manual of Macherey-Nagel GmbH & Co. KG and RNAs were detected using real-time PCR (RT-PCR).

An Applied Biosystems 7500 Fast Dx Real-Time PCR Instrument (version 2.19 software) was used for SARS-CoV-2 gene detection. A template of 7 µl of extracted RNA was used in each reaction with TaqPath™ 1-Step Multiplex Master Mix (Thermofischer Scientific, USA).

Three controls were included: positive control (TaqPath™ COVID-19 Control); negative control (from extraction run spiked with MS2); and a no template control (NTC). Finally, results were interpreted using Applied Biosystems Interpretive Software, and Ct values for three target genes, i.e., ORF1ab, N Protein, and S Protein of SARS-CoV-2, were detected along with MS2 as an internal control.

The samples were considered as positive if at least two genes showed amplification. Average Ct-value of a given sample was then converted to gene copy numbers considering the equivalence of 500 copies of SARS-CoV-2 genes as 26 Ct-value (provided with the kit), and the same was extrapolated to derive approximate copies of each gene. The average effective genome concentration present in a given sample was calculated by multiplying the RNA amount used as a template with the enrichment factor for each sample.

To determine if contamination occurred during transport, blanks in the same type of bottle were analysed prior to sampling. Duplicate analysis of samples was conducted to check accuracy and precision. To ensure instrument sensitivity and check cross-contamination, blanks were run for each batch of five samples. Signals were considered significant if the J o u r n a l P r e -p r o o f signal-to noise ratio was more than three. The limit of quantification (LOQ) of the overall method was defined as sample concentration equivalent to 1 copy per reaction tube, which was 1.7 × 10 2 copies/L. We have calculated the gene copy numbers based on the positive control provided with kit i.e., 10 4 copies/µl and the final concentration of 25 copies per reaction. Based on our experience, the same positive control is providing the same Ct values for all 3 genes analysed in this study. Hence, it is evident that primer efficiency is more or less same. Relative to the Ct values of genes of positive controls, copy numbers have been calculated in test samples of different sources.

ADR analyses was carried out in triplicate for the accuracy and precision of the data generated. Tests were repeated if the standard deviation between the triplicate was higher than 10%. Statistical analysis by Student t-test was done to compare the antidrug resistance caused by all six antibiotics in year 2018 and 2020, and the results were represented by Pearson's correlation coefficient (p), whose value ranges between zero to unity. The change in percentage resistance more than 90% (p=0.10) was considered significant.

The prevalence of E. coli and environmental parameters is summarised in Table 1. In 2018, the E. coli count was highest in river sampling locations, with maximum count of 76,600 cfu (colonies forming unit) mL -1 , which was the highest among the lake and WWTP locations except for the Vasana STP. This critically high prevalence is due to the river-human interactions at the riverfront, wastewater discharge or the stagnant flow conditions near the sampling locations (Pormohammad et al., 2019) . This reported prevalence in Sabarmati River was higher than the reported prevalence in rivers of tropical countries like India and Thailand (Chatterjee et al., 2010; Kumar and Sharma, 2014; Honda et al. 2016; Hamner et al., 2007; Hu et al., 2008) . The higher recreational activities at KL location as compared to the CL J o u r n a l P r e -p r o o f location are the main cause of higher E. coli prevalence at KL (15,600 cfu mL -1 ) than CL (3467 cfu mL -1 ) (Kumar and Sharma, 2014; Ram and Kumar, 2020) . The varying E. coli prevalence at STP locations (inlet and outlet) in 2018 indicates varying amount of incoming faecal contamination and reduction ratios in the STP.

In year 2020, the E. coli prevalence at STP locations was higher than in 2018 samples ranging from 950,000 to 4,000,00 cfu mL -1 at inlet locations and 19,500 to 32,500 cfu mL -1 at outlet locations. This is attributed to the increased domestic wastewater discharge from the Covid-19 lockdown which also increased the burden on municipal WWTPs resulting in less removal of E. coli in WWTPs. It is worth noting that, this critical E. coli prevalence alarms the municipal authorities to advance the disinfection processes in WWTPs, and therefore potential human health effects could be reduced (Pormohammad et al., 2019) .Whereas, the reduced E. coli prevalence in Sabarmati River, can be attributed to the improved water quality and attenuation capacity of the river due to less human and industrial interaction.

Another reason can be the dilution level of the samples collected from river than that of lake and WWTPs (Pormohammad et al., 2019) .

Though antimicrobials and antibiotics is among the most essential medical interventions, increased antimicrobial resistance threatens the success of patient treatment. Antibiotic resistance has been listed as one of the three major threats to the public health in 21 st century by world health organisation (WHO) (WHO, 2014) . Thus, to understand and reduce the consequences of antibiotic resistance, we need to understand its mechanism. Antimicrobial resistance is expected to be the result of the environmental interactions of the several organisms. As most of the antimicrobials consists of naturally produced compounds in nature, many of the bacteria have overcoming molecular mechanism to overcome the drugs thereby being intrinsically resistant to antimicrobials (Blair et al., 2015; Munita and Arias, 2016) .

However, we are here dealing with the acquired resistance by the bacteria which were originally susceptible to the particular antimicrobial.

Summarizing the molecular and biochemical mechanisms of antibiotic resistance is shown in Fig. 2 (Munita and Arias, 2016) . These mechanism of antidrug is generally categorised based on genetic and mechanistic basis. In genetic basis, antidrug resistance can be developed due to mutational resistance, horizontal and vertical gene transfer (HGT and VGT) . Whereas, in mechanistic basis, antidrug resistance can be developed due to changes in target site, modifications of antibiotic molecule, and decreased antibiotic penetration and efflux. Fig.2 also shows that how COVID-19 spread may impact in the development of antidrug resistance. The increased pharmaceutical pollution during COVID-19 spread can increase environmental stress on bacteria or microbes causing more mutation. This catalyses both the genetic and mechanistic basis of drug resistance. Also, the higher prevalence of bacteriophage may enhance the transduction related to HGT. Thus, the highly infected regions or hotspots of COVID-19 spread around the globe have a greater probability of emergence of super bugs having multidrug resistance. Drugs like Remdesivir, Ivermectin, Azithromycin, Favipiravir, Chloroquine, Umiferovir, Ritonavir, Aspirin, and Hydroxycholoroquinine are going to remain under scanner. In 2018, no ADR was observed for any of the antibiotics at location CL and KL, except for NFX, TCE and ST at location KL. (Fig. 3 and Fig. 4) . However, significant resistance was observed to be generated for all antibiotics, except KM, at both lake locations with higher values at CL than KL. This indicates more urbanised discharge carrying antidrug resistant E.coli accumulates at the location CL. One of the major reasons for the generated resistance at CL is the occasional discharge to the CL from nearby open Pirana solid waste dumping site (Flach et al., 2018) . This call for a monitoring of urban wastewater flows being discharged to the lake ecosystem.

Among the sampled WWTP locations in year 2018, at locations VI and VO, no resistance was observed for any of the antibiotics except TCE (20% in influent) ( Fig. 3 and Fig. 4) .

Whereas, at CI location resistance for NFX, LVX, TCE, KM, was observed but only found to be increasing towards CIP and KM at location CO. These results show the increase in antidrug resistance after WWTP treatment, which was consistent as reported in the studies from Sweden and Austria (Reinthaler et al., 2003; Flach et al., 2018) . Interestingly, ADR increased significantly for all antibiotics in year 2020 at the VI and VO locations when J o u r n a l P r e -p r o o f compared to year 2018. In year 2020, ADR was observed for all antibiotics at VI and these resistances were observed to be increasing or being constant at VO locations for all antibiotics except KM (decreased by 35%) (Fig. 3 and 4) . Such high increase in the resistance in treated effluent can be attributed to long residence time of wastewater in WWTP, where E.coli is in contact with the antibiotics or antibiotic residues for long time (Sulfikar et al., 2018) . In the case of CI in year 2020, no resistance was observed towards the quinolone drugs, whereas the observed ADR for KM, ST, and TCE, was reduced significantly at CO location. However, resistance was observed to be generated for NFX and CIP at CO in year 2020. The high resistance towards quinolone drugs is attributed to the discharge having domestic origin (Threedeach et al., 2013; Auerbach et al., 2007) , because these drugs are prescribed for treatments of respiratory and urinary tract infections, their use has increased significant during the COVID-19 pandemic (Abelenda-Alonso et al., 2020; Getahun et al., 2020; Hsu, 2020) .

Overall, the domestic municipal wastewater likely possess higher concentrations of antimicrobials than any other ambient water. Aeration enhances the generation and replication of antidrug resistant E.coli if there is a high density and diversity of microbial population in a given wastewater (Ram and Kumar, 2020; Kumar et al., 2020f) . The advanced or hybrid wastewater treatment processes should be adopted to effectively remove the antimicrobials and their residue in order to reduce the possibility of resistance (Dhangar and Kumar, 2020) . Treatment technologies such as MBR-NF/UF, MBR-UV oxidation, ASgamma radiation was found to be very effective (removal efficiency: 90 to 100%) for most of the antibiotics and other pharmaceuticals (Dhangar and Kumar, 2020) .

The abundance in both antidrug resistance and E. coli count in the STPs was found to be statically related. Previously, in case of Zenne river of Belgium, the abundance of E. coli and antidrug resistance increased from upstream to downstream after merging the effluent from J o u r n a l P r e -p r o o f Brussel's WWTP (Proia et al., 2018) . Thus, proper timely monitoring should be done to track such load of E. coli and ADR while discharging the treated effluents to the river water. From the current study, it is seen that the antidrug resistance to NFX, CIP, LVX, TCE and ST is found at most sampling locations. Such ADR generated during COVID_19 requires rigorous monitoring at local and international level through wastewater based epidemiology (Kumar et al. 2020b ). However, the lack of sanitation and treatment facilities in the undeveloped and developing countries is a big challenge to monitor the spread of ADR in the environmental waters (Pormohammad et al., 2019) . Perhaps the current pandemic may accelerate the upgradation of the current status of WWTP processes to successfully tackle the pharmaceuticals and other antimicrobials and to monitor ADR (Kumar et al., 2020a) . for almost all antibiotics: Ciprofloxacin, levofloxacin, Tetracycline, kanamycin, sulfamethaxazole (except norfloxacin: 89.1% change), was observed to very significant in year 2020 than 2018, as p <0.10. This indicates that, the significant change is occurring due to the increase in mean value of percentage of ADR. Overall, the comparison of overall ADR shows the significant increase statistically in year 2020 than 2018.

The increased cases of COVID-19 is not surprisingly correlated to SARS-CoV-2 genes in waste and natural waters (Medema et al., 2020; Ahmed et al., 2020; Haramoto et al., 2020; La Rosa et al., 2020; Sherchan et al., 2020; Kumar et al., 2020; Nemudryi et al., 2020;  J o u r n a l P r e -p r o o f Kocamemi et al., 2020) . Fig. 6 represents the population under threat of COVID-19 in Ahmedabad city (as predicted by nationally authorised Arogya-Setu app), Zone-wise scenario of effective SARS-CoV-2 genome concentration (copies/L) in Ahmedabad city, sampling locations for SARS-CoV-2 RNA analyses, and ADR analyses. The population under threat of COVID-19 in various zones of the city has been predicted by Arogya-Setu app based on the confirmed cases and the population of the respective zone. Arogya-Setu is an authorised Indian COVID-19 contact tracing, syndromic mapping and self-assessment digital service provided under Ministry of Electronics and Information Technology (MeitY), India. The sampling locations were chosen so as to cover various parts of the city. Two locations of the river i.e., SB and NB are shown with their resistance increase for Norfloxacin between 2018 and 2020. These two locations fall in the central zone of the city, which was highly affected by COVID-19, as can be seen from fig. 6a and 6b . Table 2 Vinzole STP (815 copies/L), and Ranip PS (714 copies/L). The sampled river location and the lake locations encompasses in the same zones of the city. The high SARS-CoV-2 genome copies in these zones hints the potential high prescription of antimicrobial drugs as remedy to the symptoms of COVID-19. This can be the probable reason for significant increase in ADR towards most of the drugs tested at the sampling locations in these zones. This indicates that the highly infected zones of the city, due to excessive consumption of antimicrobial drugs, has significant impact on the antidrug resistance generated in the microorganisms. Overall, the spread of COVID-19 in the community has prodigious correlation with the effective J o u r n a l P r e -p r o o f genome concentration of SARS-CoV-2 and with the prevalence of ADR in environmental waters.

The present study compared the anti-drug resistance in E.coli in 2018 and 2020 with the latter prevalence of SARS-CoV-2 genes during the sampling period. Despite the correlations between increase of ADR and COVID-19 spread, more future studies with rigorous sampling events are needed to conclude about cause and effects. In addition, the concentration of pharmaceutical and personal care products (PPCPs) should be monitored to quantify the increase owing to COVID-19 and then only an effect on ADR can be quantitatively evaluated. In this study, we attempted to start a timely discussion about the likely relationships between ADR and COVID-19 spread throughout the globe. Our approach to analyse the ADR prevalence is mostly qualitative and there can be a slight possibility of both false positive and negative results. To obtain a conclusive evidence, the quantification of genetic markers for antimicrobial resistance is helpful. In addition, one time point data sometime might not be sufficient to derive a conclusion especially when samples used for ADR and SARS-CoV-2 genomes studies do not match. However, the outbreak are too sudden to plan a better research design. We recommend considering flow data for presenting gene flux or E. coli flux, rather than the concentration.

Non-fluoroquinolone drugs showed overall more resistance as compared to fluoroquinolone drugs. Tetracycline followed by norfloxacin have shown more resistance as compared to the other drugs. Despite of decrease in the prevalence of E. coli on the sampled river locations, the percentage resistance had been significantly increased in year 2020 as compared to year 2018. However, the E. coli prevalence in STP samples was increased in the order of 10 2, but the pattern of antidrug resistance was not consistent. Lake locations also exhibited increase in J o u r n a l P r e -p r o o f the antidrug resistance during the duration of pandemic. The river locations and the lake locations have shown significant increase in the antidrug resistance and these locations are from the highly COVID-19 infected zones of the city. The COVID-19 spread in various zones of the city has shown corresponding changes in the SARS-CoV-2 genome concentration and ADR in environmental waters. Overall, due to the increased consumption of antimicrobials in the pandemic period, the percentage of antidrug resistance has been increased significantly. Wastewater based epidemiology can be the key tool to monitor the antimicrobials prevalence and antidrug resistance in the pandemic situations.

The authors declare no competing financial interest. World Health Organization. Yang, Y., Xu, C., Cao, X., Lin, H. and Wang, J., 2017. Antibiotic resistance genes in surface water of eutrophic urban lakes are related to heavy metals, antibiotics, lake morphology and anthropic impact. Ecotoxicology, 26 (6), pp.831-840. Zhang, S., Han, B., Gu, J., Wang, C., Wang, P., Ma, Y., Cao, J. and He, Z., 2015. Fate of antibiotic resistant cultivable heterotrophic bacteria and antibiotic resistance genes in wastewater treatment processes. Chemosphere, 135, pp.138-145. 

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Nehru Bridge) and SB (Sardar Bridge), and Lakes KL (Kankaria Lake) and CL

Conceptualization, Visualization, Project Supervision, Writing, Revision & Editing Kiran Dhangar: Data-Curation, First Draft

Revision & Editing Tushara Chaminda: Revision & Editing Pradeep Sharma: Revision & Editing Abhay Kumar: Revision & Editing Nirav Raval: Revision & Editing Vaibhab Srivastava: Sampling and Analyses; Data-Curation, First Draft, Revision & Editing Jörg Rinklebe: Revision & Editing Keisuke Kuroda: Revision & Editing Christian Sonne: Revision & Editing Damia Barcelo: Revision & Editing

This work is funded by Kiran C Patel Centre for Sustainable Development at IIT Gandhinagar, UNICEF, Gujarat and UKIERI. We also acknowledge the help received from Dr. Vaibhav Srivastava, Dr. Arbind K Patel, and other GBRC staffs who contributed towards sample and data analyses.

Year 

☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.☐The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: