key: cord-0709900-3atd47h7 authors: Doig, Christopher; Cooke, Rachel; Chua, Chyn; Leung, Teresa title: Acute respiratory distress syndrome precipitated by granulocyte colony-stimulating factor in undiagnosed Pneumocystis jirovecii pneumonia date: 2022-02-03 journal: BMJ Case Rep DOI: 10.1136/bcr-2021-242316 sha: 1b30e14c82c532ad247f8af27995839029c51370 doc_id: 709900 cord_uid: 3atd47h7 We present the case of a 62-year-old man with rheumatoid arthritis who developed a leukaemoid reaction and acute respiratory distress syndrome (ARDS) following granulocyte colony-stimulating factor (G-CSF) administration that had been given to treat neutropenia secondary to methotrexate and leflunomide toxicity. Later it was established that he had Pneumocystis jirovecii pneumonia, which was treated to complete resolution with a course of corticosteroids and antibiotics. This case highlights the potential risk of G-CSF administration in an immune compromised individual in the midst of bone marrow recovery in the context of active infection. Recognition of immune escape syndromes is vital and requires an understanding of potential triggers and risk factors. Granulocyte and granulocyte-macrophage colony stimulating factors (G-CSF and GM-CSF) cause myelopoiesis with the production of innate immune cells (monocytes, macrophages, dendritic cells and granulocytes) from bone marrow progenitor cells. Clinically, G-CSF can be utilised in patients to shorten the duration of neutropenia postchemotherapy, and thereby reduce the potential of developing life-threatening sepsis. 1 Pulmonary toxicity has been infrequently linked to G-CSF use. 2 It is typically characterised by cough, pulmonary oedema and, rarely, acute respiratory distress syndrome (ARDS). Such adverse events have been described in cases related to concomitant chemotherapeutic agents with independent pulmonary toxicities, most notably bleomycin 3 ; however, there are relatively few cases in which G-CSF administration, or the resultant immune reconstitution, are linked to the development of ARDS. 4 5 We report the case of ARDS precipitated by G-CSF used to treat methotrexate-induced myelosuppression, coinciding with the diagnosis of Pneumocystis jirovecii pneumonia. A 62-year-old man presented to the emergency department with a fall, and described 6 weeks of lethargy, night sweats and mouth ulcers, as well as a 10% weight loss over 6 months. His history was most significant for rheumatoid arthritis, type 2 diabetes mellitus, controlled hypertension, transient ischaemic attack and an excised squamous cell carcinoma of the skin. He was a former smoker with a 20 pack-year history. Relevant medications for his rheumatoid arthritis included methotrexate 20 mg weekly, folic acid 5 mg weekly, leflunomide 20 mg daily and prednisolone 5 mg daily, the doses of which had been constant for approximately 18 months. In addition, he had been recently treated for vitamin B12 deficiency by his general practitioner. Initial laboratory investigations revealed a macrocytic anaemia (haemoglobin 95 g/L, mean corpuscular volume 101 fL), leucopenia (absolute neutrophil count (ANC) 1.0×10 9 /L and lymphocyte count 0.5×10 9 /L), deranged liver function tests and acute kidney injury. The initial platelet count was normal at 171×10 9 /L. C reactive protein (CRP) and lactate dehydrogenase (LDH) were not elevated and a whole body CT scan was unremarkable. Vitamin B12 levels were adequate, consistent with recent replacement. His methotrexate was ceased, and on the third day of his admission he was presumptively treated for methotrexate toxicity with calcium folinate rescue; however, serum methotrexate levels subsequently returned as undetectable. Over the following 3 days of his admission, he developed new thrombocytopenia (with a nadir of 76×10 9 /L on day 3) and progressive anaemia requiring red cell transfusion. He also developed fever with dry cough and was commenced on intravenous ceftriaxone and flucloxacillin to treat sepsis of unknown origin. A chest radiograph was unremarkable at the time and he did not require any oxygen supplementation. ANC fell further to 0.7×10 9 /L and he was administered a single dose of subcutaneous filgrastim 300 µg on day 5. The following day, his clinical condition deteriorated acutely with persistent fevers and progressive respiratory compromise requiring high-flow oxygen supplementation. Hypoxaemia was confirmed on arterial blood gas. Chest auscultation revealed diffuse coarse crackles and CT chest demonstrated new changes of bilateral alveolar infiltrates (figure 1) without evidence of cardiac failure. As such, the diagnostic criteria for ARDS were satisfied. 6 Serial nose and throat swabs were negative for SARS-CoV-2 and other common respiratory viruses. Blood tests revealed an acute phase reaction with CRP 106 mg/L, albumin 19 g/L, LDH 316 U/L and ferritin 7060 µg/L, and a doubling of ANC to 1.4×10 9 /L and total white blood cell count of 2.2×10 9 /L. Blood film examination revealed a left shift with circulating intermediate myeloid cells and a population of large immature cells suspicious for blasts (comprising 4% of nucleated cells). Over the following 3 days, his clinical condition remained critical, requiring the maximal oxygenation that could be provided noninvasively. The serological parameters continued to worsen over this time with progressively marked hyperferritinaemia and increased LDH (ferritin peaking at 23 999 µg/L and LDH at 1445 U/L) (figure 2), along with the development of moderate hepatocellular damage. To investigate for haemophagocytic lymphohistiocytosis (HLH) and a possible underlying haematological malignancy, a bone marrow aspirate and trephine were performed that demonstrated hypocellularity with left-shifted granulopoiesis but no excess of blasts, and there was no morphological evidence of haemophagocytosis. Given the chronicity of immunosuppression with lymphocytedepleting therapies and progressive respiratory failure, an induced sputum was performed that identified P. jirovecii on PCR testing. A diagnosis of P. jirovecii pneumonia and ARDS was made; the latter exacerbated, or even potentially as a direct result of G-CSF administration and the consequential inflammatory response. Prior to the bone marrow biopsy, the differential diagnoses being considered included HLH, potentially as a consequence of an underlying haematological malignancy such as acute leukaemia. Secondary causes of HLH or macrophage activation syndrome and respiratory failure were also considered, particularly COVID-19 infection, which can produce a similar syndrome due to cytokine storm. In addition to temporary supplemental oxygen, the patient had his antibiotic treatment rationalised and was prescribed trimethoprim/sulfamethoaxazole 320/1600 mg orally three times daily for 4 weeks, and a tapering course of oral prednisolone. Over the following week, his cough resolved, oxygen requirement diminished and his liver and renal function recovered. Full blood examination normalised following an initial neutrophilia (ANC 24.8×10 9 /L), and ferritin and LDH improved (figure 2). He was discharged with a weaning course of steroids over 4 weeks, tapering to 5 mg daily until an alternative treatment for rheumatoid arthritis was selected, and he made a complete recovery. Syndromes such as ARDS, as well as systemic inflammatory response syndrome (SIRS) and haemophagocytic syndrome, result from overactivation of a dysregulated immune response and can be rapidly progressive and life-threatening. These types of immune escape syndromes are infrequently reported in association with G-CSF use. With hindsight, one can speculate that in the case presented, the individual's bone marrow was already recovering from methotrexate and leflunomide toxicity (and potentially megaloblastic anaemia due to vitamin B12 deficiency) at the time of presentation at the emergency department. G-CSF was later administered due to concern for his progressive neutropenia and deteriorating clinical state but would not usually be advised in the absence of severe neutropenia (typically ANC<0.5×10 9 /L). The development of ARDS in association with G-CSF-assisted neutrophil recovery has been described in a case series of patients with pre-existing, radiologically evident infections while receiving cancer chemotherapy. 7 A further case series described this phenomenon occurring in patients with the HLA-B51 and HLA-B52 antigens while receiving conventional chemotherapy or allogeneic haematopoietic stem cell transplant. 8 Reports of G-CSF-induced pulmonary toxicity independent of infections and malignancy are very rare, with only two case reports found. 9 10 Administration of granulocyte and macrophage colonystimulating factors, while useful, should be treated with caution as the upregulation of cytokines that increase alveolar permeability or neutrophil influx (such as TNF-α, IL-1β and IL-8) can exacerbate acute lung injury. Activated neutrophils have been implicated in the development of ARDS via effects on microvascular injury and vascular permeability. 9 Furthermore, increasing concentration of endogenous G-CSF has been associated with the severity of lung injury in ARDS and acute lung injury, both in bronchioalveolar lavage fluid (BALF) and serum, in addition to elevations in BALF IL-8. 10 11 In the era of COVID-19, an understanding of the mechanisms of tissue damage in lung disease, and the role that the innate immune system plays in this, is increasingly important. ► Exogenous granulocyte-macrophage colony stimulating factor (G-CSF) may exacerbate, or increase the risk of, pulmonary injury in patients with pre-existing or emerging respiratory disease. ► Localising the source(s) of infection can be difficult in immunosuppressed patients and Pneumocystis jirovecii pneumonia should always be considered in any patient with prolonged immunosuppression (especially with lymphopenia and/or corticosteroid use) and with respiratory symptoms. ► Balancing the risks of prolonged immunosuppression against the risk of immune overactivity with G-CSF administration presents a challenge that must take into consideration changing clinical findings. Caution should be exerted in those individuals with a recovering bone marrow postinsult, in whom fuelling the innate immune response may prove pathogenic especially where there is concurrent infection. The therapeutic use of G-CSF or GM-CSF in COVID-19 infection remains unclear but its use would seem counterintuitive given the promising trials with suppression of inflammatory cascades via inhibition of IL-1 and IL-6 with anakinra and sarilumab, respectively. 12 13 A recent randomised trial suggested that GM-CSF administration may result in reduced rates of death or critical illness; 14 however, several cases have been described in which patients with COVID-19 have suffered respiratory deterioration following GM-CSF. 15 16 This case highlights the potential risk of G-CSF administration in an immune compromised individual in the midst of bone marrow recovery in the context of active infection. Recognition of immune escape syndromes is vital and requires an understanding of potential triggers and risk factors. Filgrastim in patients with chemotherapyinduced febrile neutropenia. 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