key: cord-0709258-s206vrtg authors: Rajić, Zrinka; Beus, Maja; Michnová, Hana; Vlainić, Josipa; Persoons, Leentje; Kosalec, Ivan; Jampílek, Josef; Schols, Dominique; Keser, Toma; Zorc, Branka title: Asymmetric Primaquine and Halogenaniline Fumardiamides as Novel Biologically Active Michael Acceptors date: 2018-07-14 journal: Molecules DOI: 10.3390/molecules23071724 sha: ff81f65bce47ed0a1a6e586dc196c6543e97d861 doc_id: 709258 cord_uid: s206vrtg Novel primaquine (PQ) and halogenaniline asymmetric fumardiamides 4a–f, potential Michael acceptors, and their reduced analogues succindiamides 5a–f were prepared by simple three-step reactions: coupling reaction between PQ and mono-ethyl fumarate (1a) or mono-methyl succinate (1b), hydrolysis of PQ-dicarboxylic acid mono-ester conjugates 2a,b to corresponding acids 3a,b, and a coupling reaction with halogenanilines. 1-[bis(Dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU) was used as a coupling reagent along with Hünig′s base. Compounds 4 and 5 were evaluated against a panel of bacteria, several Mycobacterium strains, fungi, a set of viruses, and nine different human tumor cell lines. p-Chlorofumardiamide 4d showed significant activity against Staphylococcus aureus, Streptococcus pneumoniae and Acinetobacter baumannii, but also against Candida albicans (minimum inhibitory concentration (MIC) 6.1–12.5 µg/mL). Together with p-fluoro and p-CF(3) fumardiamides 4b,f, compound 4d showed activity against Mycobacterium marinum and 4b,f against M. tuberculosis. In biofilm eradication assay, most of the bacteria, particularly S. aureus, showed susceptibility to fumardiamides. m-CF(3) and m-chloroaniline fumardiamides 4e and 4c showed significant antiviral activity against reovirus-1, sindbis virus and Punta Toro virus (EC(50) = 3.1–5.5 µM), while 4e was active against coxsackie virus B4 (EC(50) = 3.1 µM). m-Fluoro derivative 4a exerted significant cytostatic activity (IC(50) = 5.7–31.2 μM). Acute lymphoblastic leukemia cells were highly susceptible towards m-substituted derivatives 4a,c,e (IC(50) = 6.7–8.9 μM). Biological evaluations revealed that fumardiamides 4 were more active than succindiamides 5 indicating importance of Michael conjugated system. Compounds bearing α,β-unsaturated carbonyl groups are Michael acceptors capable of conjugate addition, also known as Michael addition. The simplest and the best Michael acceptors are α,β-unsaturated carbonyl compounds with exposed unsaturated β-carbon atoms, such as exomethylene ketones and lactones or vinyl ketones [1] . These fragments are often used in the design of new anticancer drugs, together with others (6-methylhept-5-ene-1,4-dione, propiolamide, 4-(dimethylamino)but-2-enamide) (Figure 1 ). They assure the irreversible covalent binding to a cysteine residue of a specific protein and may modulate selectivity and potency of the drug candidate. The targeted covalent modification has emerged as a validated approach to drug discovery with the drug candidate canertinib [2] , approved drugs afatinib, neratinib and osimertinib (inhibitors of human epidermal growth factor receptors) and ibrutinib (Bruton s tyrosine kinase inhibitor) [3, 4] . A comprehensive review published by Jackson et al. gives an overview of biological activity and applicability of various Michael acceptors [5] . Michael acceptors have been explored in a prodrug strategy for cancer cell-specific targeting. In the review published by Zhang et al., two doxorubicin prodrugs with maleimide moieties have been described [6] . The maleimide component is responsible for the binding to human serum albumin. Once the drug carrier arrives at the targeted cancer tissue, doxorubicin is released from the carrier by the cleavage of hydrazone or glycosidic bond in the acidic environment of cancer cells. The prodrugs demonstrate superior anticancer efficacy than the parent drug. Michael acceptors are present in other classes of drugs. Examples of such drugs are entacapone (antiparkinsonic) [7] , dimethyl fumarate (antipsoriatic; since 2013 used in treatment of multiple sclerosis) [5] , rupintivir (experimental antiviral drug against human rhinoviruses) [8] , exemestane (cytostatic) [9] , and ethacrynic acid (diuretic) [10] . α,β-Unsaturated carbonyl group is also a motif found in plant and microbial metabolites and their semisynthetic/synthetic derivatives. Many of them are used in clinical practice or are still under the evaluation in clinical trials (vernolepin, helenalin, curcumin, pyrrocidine, fumaric and angelic acid derivatives), but some are classified as toxins [11] . Vernolepin and helenalin are sesquiterpenes with exomethylene lactones responsible for irreversible DNA polymerase inhibition. Pyrrocidine A is a 13-membered macrocyclic alkaloid produced by endophytic fungi, which directly binds to N-acetyl-L-cysteine methyl ester through the Michael-type addition and exerts both antimicrobial and cytostatic effect on leukemia HL60 cells [12] . Angelic acid ester ingenol mebutate has been identified as the most active component of Euphorbia peplus L. latex sap, effective against human nonmelanoma skin cancer [13] and actinic keratosis [14] . A gel formulation of ingenol mebutate has been recently approved for the treatment of actinic keratosis [15] and fumaric acid esters are used for the management of psoriasis [16, 17] . Curcumin is a symmetric α,β-unsaturated β-diketone extracted from Curcuma longa L., a tropical Southeast Asian plant used as a spice and in traditional Indian medicine [18] . Currently, there are 17 open clinical trials involving curcumin, of which mainly evaluate the combination of curcumin with other substances used in anticancer therapy [19] . In this paper, we report design and preparation of novel Michael acceptors, fumaric acid diamides 4a-f. We have chosen α,β-unsaturated amides because they are less electrophilic than analogous esters and better Michael acceptors [1] . Based on our previous findings [20, 21] , one of the amide bonds was achieved with a terminal amino group of primaquine (PQ), while the other with halogenanilines ( Figure 1 ). Sharing the same conjugated C=C-CO system and a benzene ring, our compounds are similar to cinnamic acid (trans-3-phenyl-2-propenoic acid) derivatives as well. Taking literature data into account [22] [23] [24] and our previous experience with PQ derivatives [20, 21, [25] [26] [27] [28] , we have assumed that the designed compounds have a high pharmacological potential. Here we report their synthesis, evaluation of antimicrobial activity on a wide spectrum of bacteria, fungi and viruses, their biofilm eradication ability, and finally, cytostatic activity against several human tumor cell lines. In this paper, we aimed to prepare and biologically evaluate new Michael acceptors 4a-f, asymmetric diamides of fumaric acid. To prove the significance of the conjugated Michael system for activity, we have also prepared a series of analogous compounds without a double bond, e.g., a series of succindiamides 5a-f (compound 5a is a reduced derivative of 4a, 5b of 4b etc.). In both series of compounds, one of the amide bonds was achieved with the primary amino group of PQ and the other one with a selected halogenaniline. In the first reaction step, mono-ethyl fumarate (1a) and mono-methyl succinate (1b) were coupled with PQ to give derivatives 2a,b using 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU) as a coupling reagent, along with N,N-diisopropylethylamine (DIEA) [29] . However, the transformation of 1a to carboxylic acid chloride and amidation with PQ gave better yields of product 2a. Hydrolysis of 2a,b by lithium hydroxide afforded the corresponding acids 3a,b, which were again coupled with halogenanilines in the presence of HATU/DIEA. The following anilines were used: 3-fluoroaniline, 4-fluoroaniline, 3-chloroaniline, 4-chloroaniline, 3-trifluoromethylaniline and 4-trifluoromethylaniline. Scheme 1 shows the synthetic pathway leading to compounds 4 and 5. Scheme 1. Synthesis of fumardiamides 4a-f and succindiamides 5a-f. New compounds are fully characterized by MS, IR, 1 H and 13 C NMR spectroscopic methods and elemental analyses. Spectral data are consistent with the proposed structures and are given in short in the Materials and Methods and in detail in the Supplementary Material. The presence of carbonyl functional groups in compounds 4 and 5 was indicated by the appearance of strong stretching vibration bands in IR spectra between ν 1677 and 1628 (amide I) and 1555 and 1515 cm -1 (amide II). PQ residue showed characteristic signals in 1 H NMR spectra: hydrogen atom CH-15 occurred between  8.52 and 8.55, methoxy group at  3.82, the hydrogen attached to chiral carbon (CH-10) as a multiplet at  3.57-3.65, a methyl group at  1.19-1. 22 . NH-1 appeared as a singlet between  9.98 and 10.75 ppm, NH-6 as a triplet at  7.86-8.55, while NH-12 as a doublet at  6.11-6.15 ppm. 13 C In this paper, we aimed to prepare and biologically evaluate new Michael acceptors 4a-f, asymmetric diamides of fumaric acid. To prove the significance of the conjugated Michael system for activity, we have also prepared a series of analogous compounds without a double bond, e.g., a series of succindiamides 5a-f (compound 5a is a reduced derivative of 4a, 5b of 4b etc.). In both series of compounds, one of the amide bonds was achieved with the primary amino group of PQ and the other one with a selected halogenaniline. In the first reaction step, mono-ethyl fumarate (1a) and mono-methyl succinate (1b) were coupled with PQ to give derivatives 2a,b using 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU) as a coupling reagent, along with N,N-diisopropylethylamine (DIEA) [29] . However, the transformation of 1a to carboxylic acid chloride and amidation with PQ gave better yields of product 2a. Hydrolysis of 2a,b by lithium hydroxide afforded the corresponding acids 3a,b, which were again coupled with halogenanilines in the presence of HATU/DIEA. The following anilines were used: 3-fluoroaniline, 4-fluoroaniline, 3-chloroaniline, 4-chloroaniline, 3-trifluoromethylaniline and 4-trifluoromethylaniline. Scheme 1 shows the synthetic pathway leading to compounds 4 and 5. In this paper, we aimed to prepare and biologically evaluate new Michael acceptors 4a-f, asymmetric diamides of fumaric acid. To prove the significance of the conjugated Michael system for activity, we have also prepared a series of analogous compounds without a double bond, e.g., a series of succindiamides 5a-f (compound 5a is a reduced derivative of 4a, 5b of 4b etc.). In both series of compounds, one of the amide bonds was achieved with the primary amino group of PQ and the other one with a selected halogenaniline. In the first reaction step, mono-ethyl fumarate (1a) and mono-methyl succinate (1b) were coupled with PQ to give derivatives 2a,b using 1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU) as a coupling reagent, along with N,N-diisopropylethylamine (DIEA) [29] . However, the transformation of 1a to carboxylic acid chloride and amidation with PQ gave better yields of product 2a. Hydrolysis of 2a,b by lithium hydroxide afforded the corresponding acids 3a,b, which were again coupled with halogenanilines in the presence of HATU/DIEA. The following anilines were used: 3-fluoroaniline, 4-fluoroaniline, 3-chloroaniline, 4-chloroaniline, 3-trifluoromethylaniline and 4-trifluoromethylaniline. Scheme 1 shows the synthetic pathway leading to compounds 4 and 5. Scheme 1. Synthesis of fumardiamides 4a-f and succindiamides 5a-f. New compounds are fully characterized by MS, IR, 1 H and 13 C NMR spectroscopic methods and elemental analyses. Spectral data are consistent with the proposed structures and are given in short in the Materials and Methods and in detail in the Supplementary Material. The presence of carbonyl functional groups in compounds 4 and 5 was indicated by the appearance of strong stretching vibration bands in IR spectra between ν 1677 and 1628 (amide I) and 1555 and 1515 cm -1 (amide II). PQ residue showed characteristic signals in 1 H NMR spectra: hydrogen atom CH-15 occurred between  8.52 and 8.55, methoxy group at  3.82, the hydrogen attached to chiral carbon (CH-10) as a multiplet at  3.57-3.65, a methyl group at  1.19-1. 22 . NH-1 appeared as a singlet between  9.98 and 10.75 ppm, NH-6 as a triplet at  7.86-8.55, while NH-12 as a doublet at  6.11-6.15 ppm. 13 C Scheme 1. Synthesis of fumardiamides 4a-f and succindiamides 5a-f. New compounds are fully characterized by MS, IR, 1 H and 13 C NMR spectroscopic methods and elemental analyses. Spectral data are consistent with the proposed structures and are given in short in the Materials and Methods and in detail in the Supplementary Material. The presence of carbonyl functional groups in compounds 4 and 5 was indicated by the appearance of strong stretching vibration bands in IR spectra between ν 1677 and 1628 (amide I) and 1555 and 1515 cm -1 (amide II). PQ residue showed characteristic signals in 1 H NMR spectra: hydrogen atom CH-15 occurred between δ 8.52 and 8.55, methoxy group at δ 3.82, the hydrogen attached to chiral carbon (CH-10) as a multiplet at δ 3.57-3.65, a methyl group at δ 1.19-1. 22 . NH-1 appeared as a singlet between δ 9.98 and 10.75 ppm, NH-6 as a triplet at δ 7.86-8.55, while NH-12 as a doublet at δ 6.11-6.15 ppm. 13 C spectra showed characteristic PQ signals at δ 54.92-54.98 (methoxy group), 46. .02 (C-10), 38 .46-39.23 (C-7), 33.37-33.45 (C-9), 25.70-25.99 (C-8), 20.14-20.21 (C-11) ppm and corresponding signals in aromatic region. Two signals of carbonyl groups in fumardiamides 4a-f appeared between 161.17 and 163.34 ppm, while in succindiamides 5a-f between 170.35 and 171.18 ppm. The other two succinic acid carbons (C-3 and C-4) appeared in the aliphatic region between 30.10 and 31.75, and fumaric acid carbons connected by double bond were located very high between 132.11 and 134.66 ppm. CF 3 groups in compounds 4e, 4f, 5e and 5f showed characteristic quartets at 121.32-127.05 with average J = 272 Hz, and the closest C-atom to trifluoromethyl quartets at 124-129 ppm with J = 31 Hz. m-Fluorophenyl derivatives 4a and 5a showed doublets at 162 ppm and p-fluorophenyl derivatives 4b and 5b doublets at 158 ppm (C-atom bearing fluorine) with a similar J-coupling constant of 240 Hz, while two neighbouring C-atoms appeared at 105-110 ppm (average J = 21 Hz). C-atom substituted with chloro atom appeared at 133 ppm (m-Cl) and 127 ppm (p-Cl). Chemical structures of new compounds were also supported by mass spectroscopy. Molecular ion peaks corresponding to expected relative molecular masses were obtained for all compounds. Diamides 4 and 5 were subjected to in silico analysis to evaluate the diversity of the set of compounds against topological polar surface area (TPSA) calculations and relevant drug-like properties: number of atoms, molecular weight (MW), partition coefficient (log P), H-bond donor (HBD), H-bond acceptor (HBA) and molecular refractivity (MR). The parameters are calculated with the Chemicalize.org program [30] and presented in Table S1 . All compounds are fully in agreement with the Lipinski and Gelovani rules for prospective small molecular drugs (MW ≤ 500, log P ≤ 5, number of H-bond donors ≤ 5, number of H-bond acceptors ≤ 10, TPSA < 140 Å 2 , MR within the range of 40 and 130 cm 3 /mol, the number of atoms 20-70), although 4e,f and 5e,f have the highest permitted relative molecular masses. Table 1 (the results for inactive fumardiamides and succindiamides 5a-f are not shown). In general, Gram-positive bacteria, especially S. pneumoniae and S. aureus ATCC 6538 were susceptible to 4b-f. Compounds 4a,b were active against B. cereus as well. However, Gram-negative bacterium A. baumannii was the most susceptible among all tested microorganisms: all compounds, except m-fluoro derivative 4a, showed selective antimicrobial activity against this bacterium strain. Comparison of meta and para derivatives, e.g., a vs. b, c vs. d, e vs. f revealed that in the most cases p-substituted derivatives were more active than the analogous m-derivatives. p-Chlorofumardiamide 4d showed significant activity against three bacterial strains (S. aureus, S. pneumoniae and A. baumannii), but also against C. albicans ATCC 90028, with minimum inhibitory concentration (MIC) values ranging from 6.1 to 12.5 µg/mL. This compound, together with p-fluoro and p-CF 3 fumardiamides 4b,f showed antitubercular activity against M. marinum, while 4b,f were active against M. tuberculosis as well. We tested the susceptibility of different bacterial and yeast strains to fumardiamides 4a-f and succindiamides 5a-f by determining minimum biofilm eradication concentrations (MBECs). The results are presented in Table 2 . Again, fumardiamides 4 were much more active than the analogous succindiamides 5. The most active compounds were 4a and 4b. Two microorganisms, namely E. faecalis and S. aureus showed high susceptibility to all fumardiamides, while E. coli, S. pneumoniae and P. aeruginosa were susceptible to five out of six fumardiamides. A high biofilm eradication potential of fumardiamides might be explained by the reaction of Michael acceptors with cysteine thiol, which could prevent disulfide bond formation. It is a well-known fact that cysteine homeostasis impacts biofilm formation and production of extracellular matrix components, as well as folding and stability of extracytoplasmic proteins [31] . They are also crucial for dental plaque formation, autolysis, extracellular DNA release, genetic competence, bacteriocin production and stabilization of outer membrane porin proteins [32] . Compounds 4a-f and 5a-f were evaluated against a broad variety of viral infections including herpes simplex viruses, vaccinia virus, adenovirus-2, human coronavirus (229E), vesicular stomatitis virus, coxsackie virus B4, respiratory syncytial virus, para-influenza-3 virus, reovirus-1, sindbis virus, coxsackie virus B4, Punta Toro virus and yellow fever virus. Only fumardiamides with m-chloro and m-trifluoromethyl aniline residues, namely 4c and 4e, showed significant antiviral activity against reovirus-1, sindbis virus and Punta Toro virus with EC 50 = 3.1-5.5 µM (EC 50 = concentration required to reduce virus-induced cytopathogenicity by 50%). Compound 4e was also active against coxsackie virus B4 (EC 50 = 3.1 µM). However, their selectivity index (SI), e.g., minimum cytotoxicity concentration (MCC) and EC 50 ratio, was quite low (1.8-3.7). Cytotoxicity and antiviral activity of fumardiamides 4a-f in Vero cell cultures are displayed in Table 3 (data for HEL (human erythroleukemia cell line), Hela (cervical carcinoma cell line) and Madin-Darby Canine Kidney cells (MDCK) cultures are not shown, as well as data for inactive succindiamides 5a-f). To gain insight into cytotoxicity of newly synthesized compounds 4a-f and 5a-f, their cytostatic activity was evaluated in vitro against a panel of nine different human cancer cell lines, representing various solid tumor types including pancreatic adenocarcinoma (Capan-1), chronic myeloid leukemia (Hap1), colorectal carcinoma (HCT-116), lung carcinoma (NCI-H460), acute lymphoblastic leukemia (DND-41), acute myeloid leukemia (HL-60), chronic myeloid leukemia (K-562), multiple myeloma (MM.1S) and non-Hodgkin lymphoma (Z-138). Succindiamides 5a-f were completely inactive, while free fumardiamides showed activity towards the selected tumor cell lines (Table 4) . Fumardiamide 4a with m-fluoroaniline moiety showed cytostatic activity against all tested cell lines, with IC 50 values from 5.7 to 31.2 µM (IC 50 = the lowest concentration resulting in 50% growth inhibition). DND-41 cell line was susceptible to all three m-substituted derivatives, e.g., 4a, 4c and 4f (IC 50 values between 6.7 and 8.9 µM). HL-60 and Z-138 cell lines were also susceptible to compound 4a (IC 50 values 5.7 and 8.4 µM, respectively). Glutathione (GSH) is an important compound present in most mammalian cells, a tripeptide, with the central amino acid cysteine bearing thiol group, which scavenges carcinogenic compounds by conjugate addition and protects from oxidative damage [1]. In the drug development process, it is usual to evaluate the Michael acceptor-GSH interaction [33] . That is why the final step in our research was to study the interaction of fumarmides with GSH. Fumardiamide 4b (1.25 µM) was incubated with GSH (125 µM) in ammonium formate buffer (pH = 7.4) containing 10% acetonitrile at 37 • C for 216 h. The MS analysis confirmed the consumption of 4b with GSH. However, the rate of GSH addition was slow and incomplete, as only 18.3% of 4b reacted in the monitored period (See Supporting Material). Melting points were determined on an SMP3 apparatus (Barloworld Scientific, UK) in open capillaries and were uncorrected. IR spectra were recorded on Spectrum One FT-IR (Perkin-Elmer, UK) and UV-Vis spectra on Lambda 20 double-beam spectrophotometers (Perkin-Elmer, UK). NMR 1 H and 13 C spectra were recorded at 25 • C on an NMR Avance 600 spectrometer (Bruker, Germany) at 300.13 or 600.13 and 75.47 or 150.9 MHz for 1 H and 13 C nuclei, respectively. Chemical shifts (δ) were reported in parts per million (ppm) relative to tetramethylsilane in the 1 H and the dimethyl sulfoxide residual peak as a reference in the 13 C NMR spectra (39.51 ppm). Coupling constants (J) were reported in hertz (Hz). Mass spectra were collected on an HPLC-MS/MS instrument (HPLC, Agilent Technologies 1200 Series; MS, Agilent Technologies 6410 Triple Quad) using electrospray ionization in positive mode. Elemental analyses were performed on a CHNS LECO analyzer (LECO Corporation, USA). All compounds were routinely checked by thin-layer chromatography (TLC) with Merck silica gel 60F-254 glass plates using appropriate solvent systems. Spots were visualized by short-wave UV light and iodine vapour. Column chromatography was performed on silica gel 0.063-0.200 mm. All chemicals and solvents were of analytical grade and purchased from commercial sources. PQ diphosphate, 4-methoxy-4-oxobutanoic acid (mono-methyl succinate), (E)-4-ethoxy-4-oxobut-2-enoic acid (mono-ethyl fumarate), 3-fluoroaniline, 4-fluoroaniline, 3-chloroaniline, 4-chloroaniline, 3-trifluoromethylaniline, 4-trifluoromethylaniline, DIEA, and HATU were purchased from Sigma-Aldrich. PQ was prepared from PQ diphosphate prior to use. All reactions with PQ were run light protected. PQ-succinamide and fumaramide monoesters 2a,b were prepared by condensation of PQ base with mono-methyl succinate (1a) or mono-ethyl fumarate (1b), following the previously described procedure [29] . Carboxylic acids 3a,b were prepared by hydrolysis of esters 2a,b with lithium hydroxide following the previously described procedure [29] . A solution of 0.27 mmol of 3a or 3b, 0.068 g (0.54 mmol) DIEA and 0.103 g (0.27 mmol) HATU in 6 mL of dichloromethane was stirred at room temperature. After 10 min, 0.297 mmol of the corresponding halogenaniline was added. The reaction mixture was stirred for 2-3 h at room temperature, evaporated under reduced pressure, dissolved in 8 mL ethyl acetate and extracted 3 times with water. The organic layer was dried over sodium sulfate, filtered and evaporated under reduced pressure. ν max 3381, 3298, 3068, 2959, 2928, 2863, 1635, 1591, 1521, 1465, 1419, 1386, 1331, 1210, 1163, 976 1.67, 1.63-1.53 (2m, 4H) 1.67, 1.63-1.53 (2m, 4H) (a) Staphylococci and Enterococci: The synthesized compounds were evaluated for in vitro antibacterial activity against representatives of multidrug-resistant bacteria and clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) 63718, SA 630 and SA 3202, that were obtained from the National Institute of Public Health (Prague, Czech Republic). S. aureus ATCC 29213 was used as a reference and quality control strain. In addition, all the compounds were tested for their activity against vancomycin-susceptible Enterococcus faecalis ATCC 29212 as a reference strain and three isolates from American crows of vanA-carrying vancomycin-resistant E. faecalis (VRE) 342B, 368 and 725B [34] . Ampicillin (Amp) and ciprofloxacin (CIP) (Sigma-Aldrich, St. Louis, MO, USA) were used as standards. Prior to testing, each strain was passaged onto nutrient agar (Oxoid, Basingstoke, UK) with 5% of bovine blood, and bacterial inocula were prepared by suspending a small portion of the bacterial colony in sterile phosphate-buffered saline ( Mueller-Hinton (CaMH) broth (Oxoid) for Staphylococci or brain-heart infusion for Enterococci did not exceed 2.5% of the total solution composition. The final concentrations of the evaluated compounds ranged from 256 to 0.008 µg/mL. The broth dilution micro-method, modified according to NCCLS (National Committee for Clinical Laboratory Standards) guidelines [35] in MH broth for Staphylococcus strains and CaMH for Enterococcus strains, was used to determine MIC. Drug-free controls, sterility controls, and controls consisting of MH and CaMH broths and DMSO alone were included. The determination of results was performed visually after 24 h of static incubation in darkness at 37 • C in an aerobic atmosphere. (b) Other Gram-positive (Streptococcus pneumoniae MFBF 10373, Bacillus cereus ATCC 11778, and Bacillus subtilis ATCC 6633) and Gram-negative bacteria species (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 10536, Serratia marcescens ATCC 10905, Proteus mirabilis MFBF 10430, Acinetobacter baumannii MFBF 10913, and Salmonella enteritidis MFBF 11945): Serial microdilution broth assay was used to determine MIC of compounds 4a-f and 5a-f [36] . Cell suspensions were prepared from stock cultures using phosphate-buffered saline (PBS) (Gibco Laboratories, USA) pH 7.4 and adjusted to 0.5 McFarland units using a nephelometer (ATB 1550, BioMérieux, France). Stock solutions of the tested compounds were prepared in DMSO (10 mg/mL). Testing was performed using serial dilution in microtiter flat-bottom 96-well plates with compounds ranging from 100 to 0.78125 µg/mL. After inoculation (10 7 CFU/mL) and incubation (18 h, 35 • C, aerobically in darkness), MICs for bacterial species were determined by addition of 0.5 mg/mL TTC (2,3,5-triphenyl-2H-tetrazolium chloride). The absorbance was recorded at 540 nm. As a positive quality control (susceptibility of strains) tetracycline hydrochloride (TC) (Sigma-Aldrich) was added into wells. MICs were determined as non-linear regression using GraphPad Prism as the lowest concentrations resulting in 50% growth inhibition of growth in comparison to control. (c) Mycobacteria: Mycobacterium tuberculosis H37Ra ATCC 25177 was grown in Middlebrook broth (MB), supplemented with Oleic-Albumin-Dextrose-Catalase (OADC) supplement (Difco, Lawrence, KS, USA) and mycobactin J (2 µg/mL). At log phase growth, a culture sample (10 mL) was centrifuged at 15,000 rpm/20 min using a bench-top centrifuge (MPW-65R, MPW Med Instruments, Poland). Following removal of the supernatant, the pellet was washed in fresh Middlebrook 7H9GC broth and re-suspended in fresh, ODAC-supplemented MB (10 mL). The turbidity was adjusted to match McFarland standard No. 1 (3 × 10 8 CFU) with MB broth. A further 1:20 dilution of the culture was then performed in MB broth. The antimicrobial susceptibility of M. tuberculosis was investigated in a 96-well plate format. In these experiments, sterile deionised water (300 µl) was added to all outer-perimeter wells of the plates to minimize evaporation of the medium in the test wells during incubation. Each evaluated compound (100 µL) was incubated with M. tuberculosis (100 µl). Dilutions of each compound were prepared in duplicate. For all synthesized compounds, final concentrations ranged from 1000 µg/mL to 8 µg/mL. All compounds were dissolved in DMSO, and subsequent dilutions were made in supplemented MB. The plates were sealed with parafilm and incubated at 37 • C for 7 days. Following incubation, 10% of alamarBlue (Difco, Lawrence, KS, USA) was mixed into each well, and readings at 570 nm and 600 nm were taken, initially for background subtraction and subsequently after 24 h reincubation. The background subtraction is necessary for strongly coloured compounds, where the colour may interfere with the interpretation of any colour change. For non-interfering compounds, a blue colour in the well was interpreted as the absence of growth, and a pink colour was scored as growth. The evaluation of the in vitro antimycobacterial activity of the compounds was additionally performed against M. smegmatis ATCC 700084, M. marinum CAMP 5644 and M. kansasii DSM 44162. The broth dilution micro-method in Middlebrook 7H9 medium (Difco, Lawrence, KS, USA) supplemented with ADC Enrichment (Becton, Dickinson and Comp.) was used to determine MIC as previously described [37] . The compounds were dissolved in DMSO (Sigma-Aldrich), and the final concentration of DMSO did not exceed 2.5% of the total solution composition. Final concentrations of the evaluated compounds ranging from 256 µg/mL to 0.125 µg/mL were obtained by twofold 120 • C, desolvation temperature 350 • C, desolvation gas flow 800 L/h. Mass spectra were recorded from 100-1000 m/z at a frequency of 1 Hz. Data were acquired and analysed with Waters MassLynx v4.1 software. Twelve novel PQ-derivatives of diamide type were designed and synthesized. These compounds differ in the type of spacer and/or halogen atom in aniline region. Compounds 4a-f are fumardiamides and 5a-f succindiamides . Compounds 4a,b, 5a,b are fluoro, 4c,d, 5c,d chloro and 4e,f , 5e,f are trifluoroderivatives. All new compounds were screened for antibacterial, antitubercular, antiviral and cytostatic activity as well as biofilm eradication ability. In all biological assays, fumardiamides 4 were superior to succindiamides 5, which indicates that the double bond conjugated to the carbonyl was important for the activity. With their high bioactivity, low cytotoxicity and convenient drug-like properties, p-substituted derivatives 4b,d,f provide a strong basis for further research and optimization of novel agents useful in the treatment of bacterial and biofilm-associated infections, while m-substituted derivatives 4a,c,e could be potential leads for the development of antitumor agents. The final inoculum was made by 1:1000 dilution of the suspension with sterile water. Drug-free controls, sterility controls and controls consisted of medium and DMSO Tested compounds were diluted in RPMI-1640 (Sigma) broth to concentrations 128-0.016 µg/mL. Flucytosine (FLU) (Sigma) and amphotericin B (Amph) were used as positive controls. The plates were inoculated by an inoculum prepared in RPMI-1640 broth. The final concentration of fungal cells was 5 × 10 2 -2.5 × 10 3 CFU/mL in each well. The plates were incubated at 37 • C for 24 (C. albicans, C. krusei) or 48 (C. parapsilosis) hours. Drug-free controls were included. MIC was defined as 80% or greater Absorbance was read at 540 nm. Amph was used as a positive control and solvent and media (no microorganisms added) as negative controls. 3.2.3. Minimum Biofilm Eradication Assay Biofilm eradication screening was performed on the following microorganisms: S. aureus ATCC 6538, S. pneumoniae MFBF 10373, E. faecalis ATCC 29212, B. cereus ATCC 11778, B. subtilis ATCC 6633, E. coli ATCC 10536, P. aeruginosa ATCC 27853, S. marcescens ATCC 10905, P. mirabilis MFBF 10430, S. enteritidis MFBF 11945, A. baumannii MFBF 10913, and C. albicans ATCC 90028. MBECs of fumardiamides 4a-f and succindiamides 5a-f were determined as follows [39]. Each well (96-well plate) was filled with 100 µl of bacterial (10 7 CFU/mL) or yeast (5 × 10 6 CFU/mL) suspension. When the inhibition of yeast biofilm formation was tested, the wells were pre-treated with fetal bovine serum (FBS) (250 µl per well). Negative controls contained broth only. Positive controls were performed using standard antimicrobial drugs gentamycin (Gen) and Amph, respectively. The plates were covered and incubated aerobically for 24 h (bacteria) or 48 h (yeast) at 37 • C. Following incubation period, each well was aspirated, washed three times and vigorously shaken to remove all non-adherent bacteria/yeast. The remaining attached cells were fixed with methanol (15 min) and the plates were left to dry overnight. Formed biofilm was stained with crystal violet (1%, 5 min) Antiviral Evaluation Antiviral activity of compounds 4a-f and 5a-f was determined as described previously After 3 to 10 days of incubation the virus-induced cytopathicity was determined by visual scoring of the cytopathic effect (CPE) (light microscopic evaluation of the virus-induced CPE and inhibition of evaluated compounds), as well as by measuring the cell viability with the colorimetric formazan-based MTS assay (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). All experiments were performed in duplicate After overnight incubation, cells were treated with test compounds at four different concentrations ranging from 100 to 0.8 µM. Suspension cell lines HL-60, K-562, Z-138, MM.1S, and DND-41 were seeded at densities ranging from 3000 to 10,000 cells per well in 384-well, black-walled, clear-bottomed tissue culture plates containing test compounds at the same four concentrations. The plates were incubated and monitored at 37 • C for 72 h in an IncuCyte Interaction with Glutathione (GSH) Fumardiamide 4b (1.25 µM) was incubated with GSH (125 µM) in ammonium formate buffer The aliquots were diluted 10 times with acetonitrile and sprayed at a flow rate of 50 µL/min using the fluidics system of the instrument. MS conditions were set as follows: positive ion mode, capillary voltage 3 kV Tyrosine kinase inhibitors. 17. 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The Lipinski and Gelovani parameters; Table S2 . Analytical and spectral data of compounds 4a-f and 5a-f; Table S3 . 1 H and 13 C NMR spectra of amides 4a-f and 5a-f; Table S4 . Interaction of fumardiamide 4b with GSH; Figure The authors declare no conflicts of interest.