key: cord-0704178-n9f5oqtl authors: Khan, Fahad; van den Akker, Tayler; Hussein, Shafinaz; Marcellino, Bridget K.; Khattar, Pallavi; Glicksberg, Benjamin S.; Nadkarni, Girish N.; Firpo-Betancourt, Adolfo; El Jamal, Siraj M. title: Activation of cytotoxic T cell population and inversion of CD4:CD8 ratio as manifestations of cellular immune response in SARS-COV-2 infection date: 2020-07-02 journal: J Hematop DOI: 10.1007/s12308-020-00405-9 sha: e769b0ade43d613ee7215ce3ffd18920e9ced7a4 doc_id: 704178 cord_uid: n9f5oqtl nan To the Editor, Amid the recent worldwide coronavirus disease 2019 (COVID-19) outbreak caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there has been increasing interest in the host-pathogen interaction and the resulting immune dysregulation. The role that the innate immune system plays in responding to SARS-CoV-2 is yet to be fully understood. The effect of this virus on the different lymphocyte populations is based on single-case reports and sporadic series [1] [2] [3] [4] [5] . Here, we report the findings from our evaluation of the cellular immune response in six patients infected with SARS-CoV-2 from New York City. We used flow cytometry to analyze helper and cytotoxic T cell populations, assess the antibody-secreting cells (ASCs) and examine the expression of activation markers. Briefly, we reviewed flow cytometry studies from six patients who were confirmed positive for the infection by SARS-CoV-2 using nucleic acid testing via RT-PCR of throat swab specimens using RT-PCR. All patients tested positive for SARS-CoV2 within 14 days of the flow cytometry study from our institution, a major tertiary academic center in New York City, which has been a hot spot of the COVID-19 pandemic. Originally, five of the flow cytometry studies were performed as a workup for hematological malignancies. Five peripheral blood and one bone marrow aspirate samples are included. Flow cytometry was performed by five-color analysis on the Navios Flow Cytometer (Beckman Coulter, Miami, FL), and data analysis was performed on the Kaluza Flow Cytometry Analysis Software (Beckman Coulter, Miami, FL). Leukocytes were stained with antibodies against CD3, CD4, CD8, CD19, CD38, and HLA-DR. Our initial measurements defined both CD4 + and CD8 + T cell populations. We calculated the CD4:CD8 ratio. Then, we assessed the expression of CD38 and HLA-DR on both populations as surrogate markers of activation. To screen for ASCs, we elected to use a simplified two-antibody gating strategy by evaluating the expression of CD38 on the CD19 + cells taking into account that this phenotype is expressed in most ASCs regardless of their type. Four out of the six patients included in this study showed varying degrees of lymphopenia (Table 1) . Four patients showed characteristically low CD4:CD8 ratio; these four patients showed bright expression of CD38 with partial/dim HLA-DR on the CD8 + T cells indicative of cellular activation ( Fig. 1) . Analysis of the CD19 + cells for CD38 expression showed no increase in the number of ASCs in any of the patients. The remaining two patients with normal CD4:CD8 ratio showed no expression of CD38 or HLA-DR on the T cells and no evidence of increased ASCs. Although five of the cases had a history of hematologic malignancy, only one case (patient 1) was found to have hematologic neoplasm at the time of the study. For this case, the abnormal population was detected by flow cytometry, whereby this patient had a large B-lymphoblast population (first-time diagnostic study). None of the patients was on treatment for malignancy at the time of study. In this study, we report some of the characteristics of the cellular immune response to the SARS-CoV-2 virus infection, as seen in six patients in New York City. Our results demonstrate that a subset of SARS-CoV-2-infected patients shows changes in the T cell population, evident by the decrease in the CD4:CD8 ratio and activation of the cytotoxic T cell population in the setting of lymphopenia. The decrease in the CD4:CD8 ratio is likely due to suppression in the CD4 + T cells. The current observation of lymphopenia is in accordance with the findings from previous reports studying SARS-CoV-2 [3, 4] . Lymphopenia is seen in infections by SARS, measles, and H5N1 influenza viruses and in contrast to viruses with lymphocytic proliferative responses like early human immunodeficiency virus (HIV-1), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) [6] . Although the absolute total number of lymphocytes and the absolute lymphocyte subset numbers are decreased, an inverted CD4:CD8 ratio is likely due to the greater reduction of the helper CD4 + T cell population. Moreover, the cytotoxic CD8 + T cell population shows features of activation by expressing CD38 and, to a lesser extent, HLA-DR. These features are identical to what was described in patients with influenza, Ebola, HIV, and CMV infections. The activation of the CD8 + population suggests that similar to the aforementioned viruses, the cytotoxic T cell population probably plays an integral role in the immune response to SARS-CoV-2 infection [1, 3, 4, 7, 8] . We also speculate that the decrease in the CD4:CD8 ratio and the activation of the cytotoxic T cells happen synchronously. While proliferation of ASCs would be expected in SARS-CoV-2 patients, we did not detect such an increase in any of our patients. This could be attributed to the possibility that our results provide a snapshot in the natural history of the disease based on a single reading and we do not exclude the possibility of elevated ASC numbers at different time points. Additionally, a small minority of ASCs may not be detected by the simplified CD38 and CD19 panel that we used. There are several limitations to our study. First, this is a retrospective, single-center study, with a small study sample. Second, the absence of temporal follow-up evaluations restricts understanding the natural history of the disease and the changes seen in the different studied parameters. Lastly, the underlying hematologic malignancy acting as a confounder is possible as many hematologic malignancies affect the T cell population. However, given that only one case had an active neoplastic disease, and the fact that the changes we report here are not common in hematologic malignancies, it is unlikely that this is actually confounding the results. In fact, hematologic malignancies including, acute leukemia and plasma cell dyscrasia, are associated with a heterogeneous T cell reaction characterized by decreased levels of naive CD4 + and CD8 + T cells and increasing the numbers of the so-called CD4 + /CD25 + regulatory T cells (Tregs) [9] . Despite these limitations, by using flow cytometry, we have confirmed and elaborated on the T cell compartment alterations found in the setting of SARS-CoV-2 infection [1] [2] [3] [4] 10] . In conclusion, we document the presence of a distinctive T cell response in SARS-CoV-2 patients evident by alteration of CD4:CD8 ratio, likely due to suppression of the CD4 population and activation of the cytotoxic CD8 + T cell population. Although these findings should be interpreted in the context of the study limitations, they provide potential immune parameters for further detailed studies to elucidate the immune reaction to SARS-CoV-2 virus. Breadth of concomitant immune responses prior to patient recovery: a case report of non-severe COVID-19 Pathological findings of COVID-19 associated with acute respiratory distress syndrome Characteristics of peripheral lymphocyte subset alteration in COVID-19 pneumonia Dysregulation of immune response in patients with COVID-19 in Wuhan. China Atypical lymphocyte morphology in SARS-CoV-2 infection Significant changes of peripheral T lymphocyte subsets in patients with severe acute respiratory syndrome Human Ebola virus infection results in substantial immune activation Increased expression of CD8 marker on T-cells in COVID-19 patients Regulatory T cells and their prognostic relevance in hematologic malignancies Publisher's note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations Conflict of interest The authors declare that they have no conflict of interest.