key: cord-0703270-ftkplw2v authors: Nguyen, T. D.; Bottreau, E.; Aynaud, J. M. title: Effect of deoxycholate, amphotericin B and fongizone on transmissible gastroenteritis coronavirus date: 1987-09-30 journal: Annales de l'Institut Pasteur / Virologie DOI: 10.1016/s0769-2617(87)80019-9 sha: d60352f73ff8e6d3e3bb861e39c5664fb89cc53e doc_id: 703270 cord_uid: ftkplw2v Summary At a concentration of 2 μg/ml, neither amphotericin B nor deoxycholate had an inactivating effect upon transmissible gastroenteritis Coronavirus in-fectivity. However, amphothericin B stimulated plaque formatin in agarose and facilitated the entry of viral RNA into swine testis cells. The combination of amphotericin B + deoxycholate inactivated virus infectivity and induced a decrease in plaque diameter. Finally, in the presence of these agents, the production of infectious virus and interferon was unchanged. Fongizone is a clinical formulation of amphotericin B (AmB) combined with deoxycholate (DOC) as a stabilizer, and has been widely used as an antifungal agent. The antiviral effects of AmB result not only from an alteration of the viral envelope by its combining with sterol componants [9] but also from enhancement of interferon (IFN) production in host cells [2] . Human coronavirus has been found to be inactivated by AmB [6] . On the other hand, AmB reportedly enhances viral RNA infectivity [3] . Since cell cultures for virological studies very often require antibiotics for prevention of fungus contamination, our aim was to further clarify the effect of these compounds, namely fongizione, AmB and DOC, upon virus infectivity using the transmissible gastroenteritis coronavirus (TOEV). Submitted September 6, 1986 , accepted 19 March, 1987 (*) Present address: National Institute of Veterinary Research, Nga Tu Vong-Dong Da, Hanoi (Vietnam). (**) To whom all correspondence should be addressed. Chemicals. -Fongizone (Biopro, Mulhouse, France) was used as indicated by the supplier in the state of its delivery. ArnB (Sigma, Mo, USA) was dissolved in ethanol and used immediately. DOC was from Sigma. DEAE-dextran was purchased from Pharmacia (M W 500,000) and proteinase K (Boehringer, Mannheim, Western Germany) was dissolved (l mg/rnl) in distilled water and kept at -70°C. Cells and virus. -TOEV strai ns Purdue-US, D-52 and 188-S0, as well as swine testis (ST) and pig kidney (RPD) cell lines have been described elsewhere [1] . Virus stock was prepared by growing TOEV in RPD cells. The plaque assay of TOEY carried out on confluent monolayer ST cells in 6-welJ plastic plates (Costar) has also been described [1] . Effects of fongizone, ArnB and DOC. -TOEV was prepared (about 5 x 10 2 PFU per ml) in appropriate concentrations of these compounds. After the desired times of incubation at 37°C, 0.2-ml volumes of the preparation were removed and inoculated onto confluent monolayer cells. Following virus-cell incubation for 1 h at 37°C, the inocula were removed by washes with minimal essential medium (MEM), and agarose-overlay medium without serum was replaced for plaque formation. Virus suspensions treated with MEM and/or ethanol (at the same concentration as used in the ArnB-treated virus suspension) served as controls. Plaque size and numbers were also recorded when these compounds were added into agarose-overlay medium without serum to study their eff ects on virus after attachment. IFN titration. -Confluent monolayer cells in 6-well plates were infected with TOEV at a m.o.i. of 5 to 8 PFU per cell. The inocula were removed after 1 h of incubation at 37°C; fongizone and/or ArnB at different concentrations in MEM with or without 2 % bovine fcetal serum were replaced. Virus was harvested at 18 h postinoculation and titrated. IFN in the virus su spensions was also titrated [11] . TGEV-RNA infectivity. -Virus purification and phenol extraction of TGEV-RNA were performed according to the method described by Brian et 01. [4] . Precipitated viral RNA was diluted in TE buffer (10 mM Tris-HCI and I mM EDTA pH 7.2) to obtain the initial virus volume (1 ml, virus titre: 10 9 PFU/rnl). To test infectivity, the RNA solution was serially two -fold diluted in the following solutions; MEM, MEM+DEAE-dextran (l00 ug/rnl), MEM +DEAE-dextran+AmB (2 ug/ml), MEM+DEAE-dextran+fongizone (2 ug/ml), MEM+AmB and MEM + fongizone. The RNA dilutions were inoculated into the cells grown to confluent monolayers in 24-welJ plastic plates (Costar). The cells were observed daily during the following 4 days, then frozen and thawed twice; the presence of TOEV was sought and identified by a seroneutralization test using specific antibody from previous studies [1, 14] . In preliminary studies, no effects upon TGEV infectivity of either AmB or DOC separately were found at concentrations up to 5 fLg/ml. In the experiment using a concentration of 2 ug/ml of AmB or DOC alone, TOEV infectivity decreased as a function of incubation time, similar to controls. However, in the presence of fongizone, or of AmB plus DOC, the decrease in TOEV infectivity was clearly different from that of the controls ( fig. 1 ), suggesting that AmB and DOC have a virus-inactivating effect only when they are combined. Addition of fongizone, or of a combination of AmB plus DOC in agaroverlay medium, apparently did not affect the plaque number (data not shown), but the plaque size was found to be reduced proportionally to their concentrations ( fig. 2) . No difference in plaque formation was observed between the control and the experiment using DOC. However, AmB alone added in agar-overlay medium enhanced plaque formation. This enhancement was observed only in ST cells ( fig. 3) , not in RPD cells. As ethanol had been used to dissolve AmB, a control with ethanol added to the agar-overlay medium was performed which resulted in no variation in plaque numbers (data not shown). AmB or fongizone in the medium used for virus growth had no effect on either viral yield or on IFN production, as indicated by the similarity of virus and IFN titres between the experiment and the control (data not shown). However, AmB, when added with DEAE-dextran, induced virus replication from viral RNA, although the ratio of RNA titre vs. virus titre was about to-8 . However, no virus replication resulted when the RNA was diluted in other solutions (data not shown). TOEV (an enveloped virus) has been reported to be inactivated by DOC at a concentration of 0.05 070 [5, 13] . At that concentration, we also found that DOC inactivated TOEV by more than 99.99 % (data not shown). At lower concentrations « 5 fLg/ml), DOC did not inactivate TOBV infectivity. The concentration of chemicals used in the present study (2 fLg/ml) was chosen because it is usually used to prevent fungal contamination in cell cultures. AmB (at a lower concentration than 5 fLg/ml) also did not inactivate TOBV infectivity. However, when AmB and DOC were combined, they were found to have an effect similar to that of fongizone, These results are consistent with those observed by DeVald et al. [6] and Kessler et al. [10] , but not with results obtained by Jordan and Seet (1978) , who claimed that fongizone at a concentration of 2 fLg/ml did not affect enveloped virus infectivity. However, Kessler et al. [10] claimed that AmB inactivated hepatitis B virus, when they used fongizone, a compound comprising AmB and DOC. It can be assumed that AmB alters the viral envelope by creating pores, similar to treated eukaryote cell membranes [7, 8, 15] . However, this alteration is not sufficient to inactivate virus infectivity; DOC is required to complete inactivation once these pores have been produced, or vice-versa. A synergic effect may also be the mechanism of action of the two substances. TOEV is known to induce IFN [11, 12] ; in our study, IFN production was found to be unchanged by the addition of fongizone or AmB (table I) ; therefore, virus inactivation cannot be explained by an enhanced production of IFN which, in turn, inactivated virus multiplication. Added alone to agar-overlay, ArnB enhanced plaque formation in ST cells but did not enhance virus multiplication, as indicated by similar virus titres in both the presence and absence of AmB (table I) . Along with the results of viral RNA infectivity in the presence of ArnB, this suggests a facilitating action of AmB for intracellular penetration of viral RNA rather than an enhancement of synthesis of viral components. These results are in agreement with observations by Borden et al. [3] . Finally, plaque formation was enhanced by ArnB in ST but not in RPD cells. In recent studies (to be published), we found that with similar inocula, TOEV induced a plaque number nearly three-fold higher in ST cells than in RPD cells, independently of the virus strain used. In the present study, viral RNA penetration was found to be facilitated by ArnB, and therefore more plaques were observed in ST cells. It is possible that a similar reaction occurred in RPD cells, but virus replication remained limited in these cells. However, there is no satisfactory explanation for the enhancing effect of AmB on plaque formation when DOC was jointly added, nor can we explain why, in contrast, AmB plus DOC (fongizone) in agar-overlay medium inactivated TOEV and therefore limited plaque size development, but did not do so in the liquid MEM used for virus growth. In conclusion, AmB and DOC were found to inactivate TOEV infectivity at a concentration of 2 f.l.g/ml each, while alone at that concentration, neither had such an effect. Moreover, AmB enhanced viral RNA penetration. Transmissible gastroenteritis (TOE) of swine: survivor selection of TOE virus mutants in stomach juice of adult pig Effects of amphotericin and its methyl ester on the antiviral activity of polyinosinic:polycitidylic acid Enhancement of infectivity of encephaIomyocarditis virus RNA by amphotericin B methyl ester Genome of porcine transmissible gastroenteritis virus A cytopathic virus causing transmissible gastroenteritis of swine. -1. Isolation and properties Amphotericin inhibits coronavirus SD, SK and A59 growth Aqueous pores created in thin lipid membrane by the polylene antibiotic nystatin and amphotericin B Fungal sterol and the mode of action of the polyene antibiotics Antiviral effects of amphotericin B methyl ester. A ntimicrob. Agents a. 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