key: cord-0701594-1f9n2e87
authors: Tang, Chao; Li, Lan; Mo, Tong; Na, Jintong; Qian, Zhangbo; Fan, Dianfa; Sun, Xinjun; Yao, Min; Pan, Lina; Huang, Yong; Zhong, Liping
title: Oncolytic viral vectors in the era of diversified cancer therapy: from preclinical to clinical
date: 2022-05-25
journal: Clin Transl Oncol
DOI: 10.1007/s12094-022-02830-x
sha: 4e5d3039d7d1c586c580663b14902859b9ca8408
doc_id: 701594
cord_uid: 1f9n2e87

With the in-depth research and wide application of immunotherapy recently, new therapies based on oncolytic viruses are expected to create new prospects for cancer treatment via eliminating the suppression of the immune system by tumors. Currently, an increasing number of viruses are developed and engineered, and various virus vectors based on effectively stimulating human immune system to kill tumor cells have been approved for clinical treatment. Although the virus can retard the proliferation of tumor cells, the choice of oncolytic viruses in biological cancer therapy is equally critical given their therapeutic efficacy, safety and adverse effects. Moreover, previously known oncolytic viruses have not been systematically classified. Therefore, in this review, we summarized and distinguished the characteristics of several common types of oncolytic viruses: herpes simplex virus, adenovirus, measles virus, Newcastle disease virus, reovirus and respiratory syncytial virus. Subsequently, we outlined that these oncolytic viral vectors have been transformed from preclinical studies in combination with immunotherapy, radiotherapy, chemotherapy, and nanoparticles into clinical therapeutic strategies for various advanced solid malignancies or circulatory system cancers.

Oncolytic virus (OV) is a biologically effective preparation that can enter the body through intravenous drip, intratumor injection, intraspinal drip and other methods of administration to enter the body to play a role. It has the potential to treat solid tumors or non-solid tumors [1] . OVs can selectively proliferate and replicate in tumor cells, thereby activating the host's anti-tumor immune system, and recruiting more effector lymphocytes in the tumor microenvironment to achieve the effect of killing tumor cells [2] , the mechanism can be seen in Fig. 1 . At the end of the twentieth century, it was reported that the genetically edited herpes simplex virus (HSV) could inhibit the growth of malignant glioma [3] . In the following decades, with the rapid Fig. 1 Schematic diagram of anticancer mechanism of oncolytic virus. OVs can enter the tumor site through various routes of administration (such as intratumoral injection or intravenous injection).

Oncolytic viruses that enter the tumor site can first cause immunogenic death of tumor cells through direct oncolysis (1) , release a variety of tumor-associated antigens, after being taken up by DC (2) , enter the lymph nodes through lymphatic reflux, present tumor antigen information to naive T cells in the lymph nodes, induce differentiation to produce tumor-specific effector T cells cells (3) , which acti-vate the tumor immune response. With the continuous oncolysis and T cell-specific immune attack, more tumor-associated antigens are released, and more effector T cells are generated through the presentation of DCs, causing a cascade of specific tumor immune responses (4) . In addition, memory T cells distributed in lymph nodes or local inflammation sites of tumors will conduct immune tracking of distant tumors, differentiate into effector T cells to kill tumor cells, and reduce malignant metastasis and recurrence (5) development of reverse genetic manipulation technology, gene-edited OVs also showed broad application prospects [4, 5] . In 2015, the American pharmaceutical manufacturer Amgen prepared a gene-edited attenuated HSV-1, which can be injected into the tumor to treat unresectable melanoma recurring after surgery, and it was approved by the U.S. Food and Drug Administration [6] . This is the first appearance of OV therapy among many anti-tumor therapies, and it is also a major milestone in the field of OV research. Given that the favorable anti-tumor efficacy of the virus in clinical trials, phase 3 clinical trials in combination with PD-1 antagonists have been carried out [7] . Biotechnology has promoted the research progress of OVs and deepened people's understanding of the mechanism of OVs and tumor molecular mechanisms. This review discusses several OVs that have been studied in depth and their characteristics, and analyzes the progress of these OVs in clinical applications.

As a new force in tumor immunotherapy, more and more viral vectors are being developed to obtain the best therapeutic effect. The following lists several main representative OVs and their structural characteristics. More OVs that are still in the preclinical research stage are shown in Table 1 .

As a member of the alpha-herpesvirus subfamily, herpes simplex virus (HSV) contains a double-stranded DNA genome of approximately 125-250 kb that encodes more than 70 proteins [18] (Fig. 2A) . Knockout or mutation of HSV gene RL1 by gene editing technology can reduce the expression of neurotoxic protein ICP34.5, greatly reducing the neurotoxicity of HSV [19] , making it selectively used in interferon-deficient cancer cells copying [20] . The genome of HSV is large and easy to modify. Multiple foreign genes can be inserted to improve the anti-tumor effect [21] , and the modification of the glycoprotein on the surface of HSV can improve tumor targeting [22] . The key to using HSV for oncolytic therapy is genetic engineering technology, which is to knock out the main genes that infect normal cells and replicate and proliferate in normal cells to improve the targeting of HSV infecting tumor cells, and more in tumor cells selective replication. Currently known HSV mutants that can be used as oncolytic vectors are as follows:

(1) Dlsptk, the first known type 1 HSV mutant, has a mutation in its UL23 gene, which can encode thymidine kinase and restrain the metastasis of glioma in mice [23] , but increasing the dose will cause serious encephalitis and other adverse reactions [24] ;

(2) R3616 is derived from HSV-1(F) strain, knocking out two genes encoding ICP34.5 can effectively enhance the anti-tumor ability in vivo [25] . Relevant experiments have shown that R3616 can infect tumor antigen-specific lymphocytes, not only can kill the primary tumor, but also have an inhibitory effect on distant metastasis and recurrence [26] ; (3) HSV1716, sourced from the HSV-1 (17 +) strain, deletes two copies of the neurotoxicity-determining gene RL1. Protein kinase R (PKR) phosphorylates not only eukaryotic initiation factor 2a (eIF2a), thereby inhibiting protein translation and inducing tumor cell apoptosis, but also viral death [27] . The ICP34.5-mediated dephosphorylation of eIF2a can promote protein translation and inhibit cell apoptosis, so HSV1716 can be used in tumor cells with uncontrolled protein synthesis [28] ; (4) G207 was the first HSV to be used in oncology clinical trials. The LacZ gene was inserted into the deleted ICP34.5 locus, allowing the virus to selectively replicate in malignant cells. The deletion mutant ICP34.5 induces downregulation of late viral genes including US11 through PKR [29] . G207 induces systemic antitumor immunity in the host and activates cytotoxic T lymphocytes (CTL) [30] ; (5) G47Δ, isolated from the G207 strain of HSV, has mutations in RL1 and ICP47 in its genome, and inserts the LacZ gene fragment into the ICP6 gene, resulting in a reduction in the large subunit of the encoding ribonucleic acid reductase, resulting in inactivation of ribonucleic acid reductase leads to further induction of selective replication of oncolytic HSV in tumor cells [31, 32] . In addition, the ICP47 mutation can effectively stimulate the specific tumor immune response by increasing the expression of major histocompatibility complex 1 (MHC-1) [33] ; (6) HF10 is deleted the 3.9 kb junction between the right end of UL and UL/IRL, resulting in the loss of UL56 expression and the enhanced expression of UL53 (gK), UL54 (ICP27) and UL55 [34] . In addition, HF10 can enhance angiogenesis and induce anti-tumor responses of cytotoxic T lymphocytes [35] . (7) Oncovex GM−CSF delete HSV genes encoding ICP34. 5 and ICP47, and a series of encoding cytokines, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), human interleukin-12 (IL-12), interferon alpha (IFN-α) and tumor necrosis factor alpha (TNFα), etc., were integrated into the ICP34.5 locus factor genes. Thereby, enhancing the anti-tumor immune function of oncolytic HSV, improving the immune response ability of antigen-specific T cells, reducing the negative immune regulation of CD4 + T cells, and achieving a specific anti-tumor effect in CD8 + T cells [36] .

Adenovirus is a DNA virus with icosahedral symmetry and no viral envelope, and it is also a major research hotspot in the field of OVs [37] (Fig. 2B) . However, the coxsackie and adenovirus receptor (CAR) expression on the surface of most tumor cells is low or absent, resulting in insufficient efficiency of the commonly used oncolytic adenovirus vector, human serotype 5 adenovirus, to infect tumor cells [38] . Therefore, some studies insert RGD (a short peptide composed of arginine, glycine and aspartic acid) into the fibers of the virus capsid [39] . RGD can specifically target the highly expressed integrin α v β 3 receptors in tumor tissues [40] , thereby enhancing the ability of oncolytic adenovirus vectors to actively target tumor cells. In addition to RGD, adenovirus can also be modified with other functional targeting groups, such as targeting CD46 that is up-regulated in breast and colorectal cancer [41] , or targeting desmoglin which is overexpressed in a variety of malignant epithelial cancers [42] . After a variety of modification methods, adenovirus can selectively replicate in malignant cells. The replication of adenovirus is initiated by the first adenovirus transcription unit E1A [43] . The product encoded by the E1A gene can separate the complex of retinoblastoma and E2F gene product, resulting in the activation of E1B, E2, E3, E4 and other early transcription units by free E2F transcription factors [44] . Most of the current oncolytic adenovirus vectors insert a mutation sequence into the E1A gene, which can destroy the retinoblastoma binding domain, so that more E2F transcription factor complexes are freed from the retinoblastoma complex to regulate transcription of adenovirus in tumor cells [45] . After ensuring that adenovirus can selectively replicate in tumor cells in large numbers, the researchers integrated various exogenous genes with the function of promoting tumor cell apoptosis into the genome of the oncolytic adenovirus vector, like TNF-related apoptosis-inducing ligand (TRAIL) [46] or adenoviral death protein (ADP) [47] , etc. Another type of gene that has been exploited is the prodrug activator, whose expression promotes the conversion of nontoxic prodrugs to toxic substances in the localized area of the tumor and is also delivered to nearby uninfected tumor cells through gap junctions between tumor cells, thus achieve better tumor suppressor effect [48] . For example, viruses capable of encoding bacterial cytosine deaminase (CD) [49] or herpes simplex virus-1 thymidine kinase (HSVtk) [50] . These encoded proteins can promote the conversion of prodrugs such as 5-fluorocytosine or orciclovir into toxic substances in cancer lesions, significantly enhancing the antitumor activity of adenovirus [51] . Adenovirus can not only kill tumor cells directly, but also produce persistent specific anti-tumor immune memory in the body, which is very important for the long-term prognosis of the body. However, the maintenance of this immune activation depends on the continuous replication of adenovirus [52] . When the tumor cells die, part of the adenovirus in the cell will also die, reducing the total number of viruses and weakening the long-term therapeutic effect. In the past ten years, the research direction of oncolytic adenovirus has evolved from the use of genes to maximize the immediate killing of tumor cells to amplify the immune response induced by viral oncolysis. Inspired by this concept, more oncolytic adenoviral vectors are loaded with immunomodulatory factors, including GM-CSF, CD40 ligands, or interferon-1 (IFN-1) [53] .

From the 1970s to the 1980s, clinicians observed a phenomenon: patients suffering from leukemia or lymphoma after infection with measles virus (MV) appeared remission [54] , based on this clinical phenomenon, MV has been gradually developed as a cancer immunotherapy. MV is spherical or filamentous, with a diameter of about 120-250 nm.

The core is a single negative-stranded RNA, which is not segmented. The full length of the genome is about 16 kb (Fig. 2C) . In subsequent research, the effective replication of MV in malignant tumor cells and the lysis and death of tumor cells mediated by MV have been confirmed in a number of basic studies [55] [56] [57] . Oncolytic MV can enter tumor cells through the mediation of cell membrane receptors such as CD46 and CD150 [58] . CD46 expression is expressed in most normal tissue cells, but because normal cells can clear the virus through the IFN anti-virus pathway, the oncolytic attenuated MV is not sufficiently amplified in normal cells to a pathogenic level [59] . Compared with normal tissue cells, most cancer cells typically highly express CD46, resulting in oncolytic MVs preferentially infecting tumor cells and spreading in tumor tissue, especially some malignant tumor cells with detective anti-virus response, such as the lack of type 1 IFN system, which is more conducive to allowing the virus to enter and spread in tumor cells. replication and proliferation [60] . The P, V, and C proteins of pathogenic MVs are the weapons of MV against the host cell IFN system, but these arms have been unloaded in oncolytic MVs, so oncolytic MVs show high sensitivity to the IFN system [61] . The sequences encoding P protein, V protein and C protein are different in different MV strains, so engineering MVs into oncolytic MVs also requires certain precise regulation [62] . However, there is no systematic study of the interaction between these differences and the efficacy of oncolytic MVs. The oncolytic effect of MV is related to the cytopathic effect. The infected tumor cells fuse into multinucleated syncytia, lose the integrity of the cell membrane, and eventually lead to cell death. MV replication, protein expression, syncytium formation and oncolytic effects have been verified in several human-derived cancer animal models, including circulatory system cancer such as leukemia [63] and myeloma [64] , as well as solids tumors such as ovarian carcinoma [65] , glioblastoma [66] , liver cancer [67] , pancreatic cancer [68] , breast cancer [69] and gastric cancer [70] .

Newcastle disease virus (NDV) is a paramyxovirus type 1 (APMV-1), which has a negative sense single-stranded RNA genome (Fig. 2D ). NDV uses poultry as its host, and has evolved a virus immune escape mechanism in the poultry body, that is, interferes with the host response mediated by IFN-1. Importantly, this viral escape mechanism is not species-specific and works neither in accepted nor non-accepted hosts [71] . Some NDV strains exhibit natural eosinophilia and immunostimulatory properties in non-receptive hosts [72] . According to the pathogenic type and virulence of NDV virus, it can be divided into chronic (low), intermediate (medium) and rapid (high) [73] .The whole genome sizes of NDV are in line with the principle of 6 times that of APMV-1. Genomic RNA contains two extragenic regions, namely a 30 bp leader region and a 50 bp trailing region [74] . These regions are used to control the transcription and replication of the virus, and are also used to embed newly synthesized RNA into virus particles. NDV has 6 genomes (3'-N-NP-M-F-HN-L-5'), N gene encodes nuclear protein, P gene encodes phosphoprotein, M encodes matrix protein, F gene encodes fusion protein, and HN gene encodes hemagglutinin-neural enzyme protein, L gene encoding and large protein [75] . It can be seen that exogenous genes with anti-tumor function can also be easily inserted into the huge genome of NDV by gene editing technology.

NDV-infected cells first connect the cell-binding domain through the HN molecule. Under the action of α2,6-sialic acid, HN and NDV bind to the surface of the host cell. After the virus binds to the cell surface, the fusion protein F is activated. The synergistic effect of HN and F causes the virus to fuse with the host cell membrane, allowing the viral genome to enter the cytoplasm of the host cell [76] . NDV can also use clathrin-mediated endocytosis and endocytosis as another way to enter cells [77] . In the cytoplasm, NDV's RNA is transcribed into messenger RNA (mRNA). Compared with mammalian mRNA, viral mRNA is still polyadenylated and carries 50-phosphate (50-PPP). Then the viral mRNA occupies the host cell's translation system and substrate to translate the genome into the corresponding viral protein. The next step is to use the newly generated nucleocapsid as the positive chain "antigenome" [78] for virus replication. The viral nucleocapsid consists of a single viral RNA (about 15,000 nucleotides in length) with NP, P, and L proteins. Binding of viral proteins P and L requires the RNA-dependent RNA polymerase, which is required for viral replication. There are 10 3 -10 4 viruses that can replicate in each cell. The newly generated viral genome is encapsulated in the plasma membrane, and new viral particles are released through a process of budding from the cell membrane.

The infection of normal cells from non-receptive hosts (such as mice or humans) usually only stops at the step where NDV mRNA is transcribed into protein, and does not enter the subsequent virus replication and proliferation process [79] . NDV infection of tumor cells is related to the cleavage site of F and the second sialic acid binding site of HN. Persistent infection can avoid direct cell lysis and death, and can mediate immunogenic cell death (ICD) of tumor cells and promote the spread of viruses between cells. This is a potential mechanism for evading host antiviral responses [80] . Wild-type and engineered NDVs can be used alone or in combination with clinically routine cancer therapies to treat human cancers [81] .

Reovirus (respiratory and enteric orphan virus) is a nonenveloped double-stranded RNA virus with a regular icosahedral structure and a diameter of about 60-80 nm (Fig. 2E) , which is widespread in nature. Reovirus is able to block mutations in Ras (PKR signaling pathway), thereby maintaining its ability to selectively infect tumor cells [82] . More importantly, human normal cells are resistant to reovirus infection and replication, so the biosafety of reovirus has been validated in both preclinical studies and clinical trials [83] .

Respiratory syncytial virus (RSV) belongs to the family Paramyxoviridae. The diameter of the virus particle is about 150-250 nm kb (Fig. 2F) . RSV can cause lesions in the human respiratory tract, but it has been reported that RSV has oncolytic ability in cancers such as prostate cancer and lung cancer [84, 85] , which may be related to the ability of RSV to directly or indirectly promote apoptosis in tumor cells or the obstruction of the IFN system in tumor cells [86] , but the specific mechanism remains to be explored.

In order to achieve satisfactory results in antitumor therapy, a surging number of novel combination therapy methods have emerged in the preclinical studies of OVs ( Table 2 ). Given that the strong ability of OV to activate host-specific anti-tumor immunity [87] , the combination therapy of OVs with immunotherapy, radiotherapy and chemotherapy or other biological materials will be a major direction in the future. (Fig. 3 ).

Tumor cells in the body often exhibit behaviors such as escaping from immune monitoring and losing immune response. Therefore, the higher the degree of malignancy, the faster the spread of cancer, and the worse the prognosis of cancer patients. These biological phenomena of tumor cells are related to the immune examination mechanism in the host, namely, programmed cell death ligand 1 (PD-L1) and programmed cell death protein 1 (PD-1) is closely related to the up-regulation of cytotoxic T lymphocyte associated protein 4 (CTLA-4) [100] . More importantly, the up-regulation of CLTA-4 and PD-1 was detected in tumor treatment after administration [101] . Therefore, the combination of viral therapy and immune checkpoint inhibitors (ICIs) will be a promising cancer treatment strategy. Despite the undisputed success of ICIs, there are still relatively few cancer patients who can benefit from a single ICI therapy. OVs can promote the anti-tumor immune response by inducing tumor cell immunogenic cell death [102] . During the oncolytic process of the virus on tumor cells, it releases [97] tumor-associated antigens (TAAs) and produces inflammatory cytokines [103] . The result is that OVs can transform the immunosuppressive "cold" tumor microenvironment into a "hot" immune stimulation environment. ICIs inhibit the relevant pathways of negative immune regulation, allowing CD4 + and CD8 + effector T cells to regain their immune function in the tumor microenvironment, while OVs release TAA by causing tumor cell immunogenic death to stimulate the tree in the tumor microenvironment dendritic cells (DCs) enhance their antigen presentation function and stimulate the maturation of primitive T cells [104] . Based on the immune properties of the above-mentioned OVs in the tumor microenvironment, anti-PD-L1/PD-1 antibody, anti-CTLA-4 antibody and other ICIs are used in combination with OVs to achieve the anti-tumor immunity. The synergistic effect of two-pronged approach to kill tumor cells. Yuan et al. developed a recombinant oncolytic adenovirus that could open the blood-brain barrier. In a glioblastoma model, the combination with PD-L1 antibody was found to synergistically improve oncolysis and induce tumor cell pyroptosis [105] . In addition, some small-molecule immune adjuvants such as Toll-like receptors and Chimeric antigen receptor T-cells (CAR-T) can also be combined with OVs, which are safe and effective [106] . Therefore, OVs and ICIs are complementary and can cascade to amplify specific anti-tumor immune effects and achieve better anti-tumor effects.

Radiotherapy and chemotherapy are still the conventional methods for the treatment of tumors in the world. Some advanced malignant tumors with poor surgical treatment, or some tumors that are sensitive to radiotherapy and chemotherapy are often treated with these methods. The anti-tumor mechanism of conventional chemotherapeutic drugs is to achieve anti-tumor effect by inhibiting DNA synthesis in the cell cycle of tumor cells [107] . Radiotherapy is to fight cancer through direct and irreparable damage to DNA by double-strand breaks. Therefore, a single chemotherapy or radiotherapy often brings certain adverse reactions to the patient, which affects the therapeutic effect and prognosis of the tumor. The OV is suitable for tumors of various stages, and its administration methods are diverse and suitable for combined administration. Nowadays, many research results have confirmed that the combined application of chemotherapeutic drugs and OVs can exert better anti-tumor effects in some human-derived xenogeneic living tumor models. This synergy has two advantages: first, it can reduce possible adverse reactions without affecting the overall efficacy of OVs; second, it can reduce the amount of OVs used under the same time and space conditions. This reduces the adverse reactions that the drug may bring to the body, and at the same time reduces the possibility of tumor cells developing drug resistance [108] [109] [110] . Cyclophosphamide, which is used to treat leukemia and some solid tumors, is the first chemotherapeutic drug used in combination with OVs [111] . With the development of more targeted drugs, the use of new, less toxic drugs in combination with OVs to treat tumors has better prospects for clinical applications. [113] . In addition to chemotherapy, radiotherapy is also a classic treatment for tumors. During radiotherapy, some signal pathways of the tumor will be activated, and some related genes can be down-regulated or up-regulated so that the OV can replicate and proliferate in tumor cells. Chen et al. investigated the synergistic antitumor effect of radiation therapy and oncolytic vaccinia virus on the basis of a mouse tumor model, in tumor-bearing mice receiving high-dose hypofractionated stereotaxic body radiotherapy (SBRT) and oncolytic vaccinia virus. After treatment, the infiltrating numbers of CD4 + and CD8 + cells in the tumor area were increased, while the infiltration of Treg cells was decreased, and the ratio of M1/M2 macrophages was increased [114] . The combined application of OV, radiotherapy and chemotherapy has demonstrated good efficacy and has been confirmed by many pre-clinical studies, and it is safer and more effective than single medication. However, according to the tumor type, size, stage, and the overall physiological condition of the host, as well as the different types of OVs and chemotherapeutic drugs, the synergistic anti-tumor effect and specific molecular mechanisms of this combined application need to be further studied.

In recent years, with the rapid development of the field of nanotechnology, the application of bio-nanomaterials in tumor therapy is receiving extensive attention [115] . They have good drug-carrying capacity. bio-imaging, activation of tumor immunity and other functions are integrated, and after a certain modification, it can be specifically targeted to tumor cells. Whether in tumor diagnosis or treatment, it shows high potential application prospects. Therefore, biological materials can be used to load OVs to achieve the purpose of blocking virus immunogenicity and achieving tumor targeted delivery. Currently used for loading OVs are mainly liposomes, cells or exosomes [116, 117] , and some non-biological material carriers such as high-molecular polymers [118] .

There are many kinds of OVs that have been clinically tested. Compared with traditional surgical treatment, radiotherapy, chemotherapy and other conventional methods of treating tumors, these OVs show a certain degree of novelty and synergistically enhance the effect of curative effect ( Table 3 ).

The following summarizes the latest clinical applications of several popular OVs.

T-Vec, an oncolytic HSV, is currently marketed for the treatment of melanoma that cannot be treated by surgical therapy [119] . A phase II clinical trial (NCT02211131) used T-Vec as an adjuvant drug after melanoma surgery 150 postoperative patients with stage IIIB-IVM1a melanoma were equally divided into two groups (with or without T-Vec postoperatively) for postoperative risk assessment of melanoma recurrence. After three years of observation, 17.1% of patients who underwent T-VEC therapy after surgery achieved pathological complete remission (pCR) and increased CD8 + in tumor lesions. Compared with the surgery alone group, postoperative adjuvant use overall melanoma recurrence was reduced by 25% in the T-Vec arm [120] . HSV1716 demonstrated well-tolerated and antitumor properties in an OV clinical trial in malignant pleural mesothelioma (NCT01721018).

Replication and presence of HSV were consistently detected in tumor lesions in 7 of 12 patients. And half of the patients reported stable disease (SD) at week eight [121] .

In 2017, Northwestern University Feinberg School of Medicine developed an engineered oncolytic adenovirus called NSC-CRAd-S-pk7, and its safety was confirmed in a 3-year phase 1 clinical trial (NCT03072134), at the maximum dose in the trial (1.875 × 10 11 viral particles), the patients had no obvious adverse reactions, and showed a certain antitumor effect, 1 of the 12 patients had partial remission (PR), and 10 had SD. Immunological examination confirmed the increase of peripheral blood circulating immune cells and the increase of CD8 + :CD4 + ratio of tumor lesions in the brain [122] . In addition to oncolytic therapy alone, oncolytic adenovirus can be used in combination with radiation therapy to enhance antitumor efficacy [123, 124] . In a phase 1 clinical trial investigating the relationship between oncolytic adenovirus and radiosensitization (NCT03213054), 13 patients with esophageal cancer who were ineligible for surgery and chemotherapy were recruited after 3 intravenous injections of OV (NCT03213054). Eight patients had local complete remission (CR) after six weeks of conventional radiotherapy on day 1, day 18, and day 32) and a total of 60 Gy, and none of the tumor biopsy specimens showed viable pathology. There were three cases of partial remission. Immunohistochemical examination of tumor tissues from all patients revealed increased PD-L1 expression and massive infiltration of CD8 + cells [125] . Astoundingly, the National Institutes of Health Clinical Center proposed the mixed use of oncolytic adenoviruses to enhance tumor targeting. They developed three oncolytic adenoviruses expressing prostate-specific antigen (PSA), Mucin-1 (MUC-1) and brachyury, respectively. The C-terminus of brachyury and MUC-1 are overexpressed in metastatic castration-resistant prostate cancer (mCRPC) and have been shown to be important in response to chemotherapy, epithelial-mesenchymal transition, and metastasis. play an important role in resistance [126] . Enrolled patients in this phase 1 clinical trial (NCT03481816) received concurrent subcutaneous injections of all three oncolytic adenoviruses (subcutaneously every 3 weeks, 5 × 10 11 viral particles per dose, up to 3 doses, followed by every 8 weeks) Booster vaccine for 1 year), one patient had a partial response, five patients had stable disease for more than 6 months, median progression-free survival was 22 weeks, and pluripotent T cells were also detected in most patients after vaccination No dose toxicity was seen in response to these three tumorassociated antigens [127] . 

In addition to solid tumors, OVs can also be used in the treatment of circulatory malignancies [128, 129] . MV-NIS is a recombinant oncolytic measles virus that encodes a sodium iodide homologue and mediated entry into tumor cells through the CD46 receptor in vivo [130] . It has been reported that myeloma plasma cells typically overexpress CD46 [131] and are sensitive to MV-NIS. Inspired by this, Stephen et al. conducted a phase 1 clinical trial (NCT00450814) in multiple myeloma. After MV-NIS treatment, the proportion of CD3 + and CD8 + T cells in the peripheral blood of the patients was significantly increased. There was also a certain increase in central memory T cells and effector memory T cells, suggesting that MV-NIS specifically captured hematological tumors and caused a systemic anti-tumor immune response, which improved the long-term prognosis of multiple myeloma [132] .

Compared with other OVs, there are relatively few clinical trials related to NDV. The oncolytic effect of wild-type NDV was reported as early as in a phase 1 clinical trial in 2002 [133] . With the maturity of gene editing technology and the development of the genetic engineering technology industry, people are also constantly developing the clinical translation application value of oncolytic NDV [134] [135] [136] . The German Cancer Center infected autologous tumor cells with NDV, and then administered an irradiation dose of 200 Gy to prepare an NDV anti-cancer vaccine, which was then combined with a bispecific anti-CD28 fusion protein to conduct phase 1 clinical trial on 14 advanced colorectal cancer patients' clinical trials. Of these, tumor-reactive T cells were detected in all patients during treatment, suggesting the ability to reactivate nonviable T cells in advanced cancer [137] . Surprisingly, the combination of NDV and different therapies showed amazing therapeutic effects. In two case reports, researchers administered NDV, local hyperthermia, NDV, and DC vaccines to a prostate cancer patient and a breast cancer patient who had failed standard therapy. Combination therapy, after continuous follow-up confirmed that the patient achieved long-term complete remission [138, 139] .

Pelareorep (Reolysin) is an oncolytic viral biological product that shows overall survival benefit by intravenous injection. It is a non-genetically modified non-pathogenic reovirus that overcomes the effect of neutralizing antibodies and activates the body's own immune system by activating the body's own immune system. It can selectively infect and destroy tumor cells and is used to treat a variety of solid tumors and hematological malignancies. In a phase I trial of Pelareorep, a median tissue culture infective dose (TCID 50 ) of 1 × 10 8 to 3 × 10 10 was divided into 6 graded doses of intravenous Pelareorep administered 4 weeks apart. No dose limited toxicity (DLT) was observed in all evaluable patients with advanced solid tumors, and treatment outcomes in 15 patients showed 1 PR and 7 SD [140] . Besides, in a clinical trial of children with extracranial solid tumors with a median age of 12.5 years (3.0-20.2 years), the safety of intravenous Reolysin at a dose of 5 × 10 8 TCID 50 /kg (once daily for 5 days) was also demonstrated [141] .

In recent years, RSV has made some progress in preclinical studies of human hepatocellular carcinoma [142] and human skin cancer [143] , but there is no clinical trial results of RSV in oncolysis research. Astonishingly, a case report found that COVID-19, the respiratory virus ravaging the world, alleviated the progression of Hodgkin's lymphoma unexpectedly, shedding a new perspective on the development of respiratory virus oncolytic engineering [144] .

Oncolytic viruses have shown broad application prospects in pre-clinical research and clinical trials. At present, patients with relapsed or refractory advanced tumors are the main participants in clinical trials of OV therapy, and almost all viral therapies are performed on patients with advanced solid tumors. In order to enhance the anti-tumor effect, a growing number of natural viruses have been developed and modified to improve the infectivity or immune activity of the virus to tumor cells. In addition, new strategies for combination therapy are constantly increasing to improve the effectiveness of cancer treatment. Although the use of most viruses and combination therapies are adopted, there are still some improvements in clinical trials of virus treatments. For example, the OV itself has strong immunogenicity, and most of them will be cleared after entering the host body, leading to aggregation The total amount of OV at the tumor site is reduced, which affects the efficacy of OV. In order to further improve the therapeutic effect of OVs and further extend the survival period of patients, it is necessary to develop more safe vectors to reduce the killing of the virus by the host's immune system. In addition, more clinical trials are needed to ensure the biological safety of OVs.

Author contribution HY guided the general direction of this review. CT wrote the original manuscript and drew the illustrations, LZ provided revisions and guidance to the manuscript. TM provided design proposals for the illustrations and made the revision of manuscript. LL and JN revised and corrected the original manuscript, ZQ, DF, and XS supported at the revision and correction of manuscript, MY and LP assisted in the drawing of illustrations.

The authors report no conflicts of interest.

Ethical statement This manuscript does not contain any original experimental data and patient data, and the preclinical studies and clinical trials cited are published results.

Informed consent statement This review did not contain any privacy of patients or original unpublished experimental data, we just discussed some clinical trials results that have been published and did not conduct any clinical trials. Furthermore, in this review, it can be found in the cited clinical trials articles that all patients have signed informed consent and agreed to participate in the clinical trial. Relevant statements can be found in the Ethical statement.

Metabolic barriers to cancer immunotherapy

Immunocompetent canceron-chip models to assess immuno-oncology therapy

Selective killing of glioma cells in culture and in vivo by retrovirus transfer of the herpes simplex virus thymidine kinase gene

Gene editing thumbs a ride with oncolytic virotherapy

Development of oncolytic virotherapy: from genetic modification to combination therapy

Oncolytic virotherapymediated anti-tumor response: a single-cell perspective

Oncolytic virotherapy promotes intratumoral T cell infiltration and improves anti-PD-1 immunotherapy

An engineered oncolytic virus expressing PD-L1 inhibitors activates tumor neoantigenspecific T cell responses

An oncolytic virus expressing a fulllength antibody enhances antitumor innate immune response to glioblastoma

A modular self-adjuvanting cancer vaccine combined with an oncolytic vaccine induces potent antitumor immunity

An immune-competent, replication-permissive Syrian Hamster glioma model for evaluating Delta-24-RGD oncolytic adenovirus

Viral infection of cells within the tumor microenvironment mediates antitumor immunotherapy via selective TBK1-IRF3 signaling

Genetically engineered cell membrane nanovesicles for oncolytic adenovirus delivery: a versatile platform for cancer virotherapy

Systemic cancer therapy with engineered adenovirus that evades innate immunity

Repurposing rotavirus vaccines for intratumoral immunotherapy can overcome resistance to immune checkpoint blockade

An oncolytic herpesvirus expressing E-cadherin improves survival in mouse models of glioblastoma

Re-designing Interleukin-12 to enhance its safety and potential as an anti-tumor immunotherapeutic agent

CRISPR/Cas9 genome editing technology significantly accelerated herpes simplex virus research

Histopathological responses in the CNS following inoculation with a non-neurovirulent mutant (1716) of herpes simplex virus type 1 (HSV 1): relevance for gene and cancer therapy

Interferon-independent activities of mammalian STING mediate antiviral response and tumor immune evasion

Development of a new fusion-enhanced oncolytic immunotherapy platform based on herpes simplex virus type 1

The structural basis of herpesvirus entry

Experimental therapy of human glioma by means of a genetically engineered virus mutant

Reduction and elimination of encephalitis in an experimental glioma therapy model with attenuated herpes simplex mutants that retain susceptibility to acyclovir

A novel multiplymutated HSV-1 strain for the treatment of human brain tumors

Oncolytic viruses derived from the gamma34.5-deleted herpes simplex virus recombinant R3616 encode a truncated UL3 protein

Intralesional injection of herpes simplex virus 1716 in metastatic melanoma

Macrophages mediate the antitumor effects of the oncolytic virus HSV1716 in mammary tumors

Attenuated multi-mutated herpes simplex virus-1 for the treatment of malignant gliomas

Oncolytic HSV-1 G207 immunovirotherapy for pediatric high-grade gliomas

Macrophage polarization contributes to glioblastoma eradication by combination immunovirotherapy and immune checkpoint blockade

Rad51 degradation: role in oncolytic virus-poly(ADP-ribose) polymerase inhibitor combination therapy in glioblastoma

Oncolytic herpes simplex virus vector with enhanced MHC class I presentation and tumor cell killing

Genomic signature of the natural oncolytic herpes simplex virus HF10 and its therapeutic role in preclinical and clinical trials

Intratumoral injection of herpes simplex virus HF10 in recurrent breast cancer

Local delivery of OncoVEX generates systemic antitumor immune responses enhanced by cytotoxic T-lymphocyte-associated protein blockade

Oncolytic adenovirus programmed by synthetic gene circuit for cancer immunotherapy

Enhanced therapeutic efficacy of an adenovirus-PEI-bile-acid complex in tumors with low coxsackie and adenovirus receptor expression

Phase I study of DNX-2401 (Delta-24-RGD) oncolytic adenovirus: replication and immunotherapeutic effects in recurrent malignant glioma

Molecular magnetic resonance imaging of Alpha-v-Beta-3 integrin expression in tumors with ultrasound microbubbles

Membrane-bound complement regulatory proteins are prognostic factors of operable breast cancer treated with adjuvant trastuzumab: a retrospective study

A randomized trial of TLR-2 agonist CADI-05 targeting desmocollin-3 for advanced non-small-cell lung cancer

Human DNA-PK activates a STING-independent DNA sensing pathway

BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains

Systemic cancer therapy with engineered adenovirus that evades innate immunity

Enhancing the antitumor activity of an engineered TRAIL-coated oncolytic adenovirus for treating acute myeloid leukemia

An oncolytic adenovirus 11p vector expressing adenovirus death protein in the E1 region showed significant apoptosis and tumour-killing ability in metastatic prostate cells

Immunotherapeutic intervention with oncolytic adenovirus in mouse mammary tumors

A bicistronic adenoviral vector carrying cytosine deaminase and granulocyte-macrophage colony-stimulating factor increases the therapeutic efficacy of cancer gene therapy

Long-term treatment with valganciclovir improves lentiviral suicide gene therapy of glioblastoma

Re-designing Interleukin-12 to enhance its safety and potential as an anti-tumor immunotherapeutic agent

Viral and cellular interactions during adenovirus DNA replication

Efficacy of recombinant adenovirus expressing a fusion gene from GM-CSF and Epstein-Barr virus LMP2A in a mouse tumor model

Mechanisms of measles virus oncolytic immunotherapy

Immunovirotherapy with measles virus strains in combination with anti-PD-1 antibody blockade enhances antitumor activity in glioblastoma treatment

Oncolytic measles virus efficacy in murine xenograft models of atypical teratoid rhabdoid tumors

An oncolytic measles virussensitive Group 3 medulloblastoma model in immune-competent mice

p53 regulates CD46 expression and measles virus infection in myeloma cells

Oncolytic measles virus as a novel therapy for malignant peripheral nerve sheath tumors

Frequent homozygous deletions of type I interferon genes in pleural mesothelioma confer sensitivity to oncolytic measles virus

Interleukin-13 displaying retargeted oncolytic measles virus strains have significant activity against gliomas with improved specificity

Rapid identification of measles virus vaccine genotype by real-time PCR

Attenuated measles virus controls pediatric acute B-lineage lymphoblastic leukemia in NOD/SCID mice

Oncolytic measles virus therapy enhances tumor antigen-specific T-cell responses in patients with multiple myeloma

Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancer

Measles virus-based treatments trigger a pro-inflammatory cascade and a distinctive immunopeptidome in glioblastoma

Oncolytic measles virus enhances antitumour responses of adoptive CD8(+)NKG2D(+) cells in hepatocellular carcinoma treatment

Oncolytic virus with attributes of vesicular stomatitis virus and measles virus in hepatobiliary and pancreatic cancers

Recombinant SLAMblind measles virus is a promising candidate for nectin-4-positive triple negative breast cancer therapy

A recombinant measles virus vaccine strain rMV-Hu191 has oncolytic effect against human gastric cancer by inducing apoptotic cell death requiring integrity of lipid raft microdomains

Signaling through RIG-I and type I interferon receptor: Immune activation by Newcastle disease virus in man versus immune evasion by Ebola virus (Review)

Persistent Newcastle disease virus infection in bladder cancer cells is associated with putative pro-survival and anti-viral transcriptomic changes

Updated unified phylogenetic classification system and revised nomenclature for Newcastle disease virus

Breaking therapy resistance: an update on oncolytic newcastle disease virus for improvements of cancer therapy

Immunobiology of newcastle disease virus and its use for prophylactic vaccination in poultry and as adjuvant for therapeutic vaccination in cancer patients

6-linked sialic acid serves as a high-affinity receptor for cancer oncolytic virotherapy with Newcastle disease virus

Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells

Newcastle disease and related avian paramyxoviruses

Tumor selective replication of Newcastle disease virus: association with defects of tumor cells in antiviral defence

Newcastle disease virus establishes persistent infection in tumor cells in vitro: contribution of the cleavage site of fusion protein and second sialic acid binding site of hemagglutinin-neuraminidase

Use of attenuated paramyxoviruses for cancer therapy

The molecular basis of viral oncolysis: usurpation of the Ras signaling pathway by reovirus

Intravenous administration of Reolysin®, a live replication competent RNA virus is safe in patients with advanced solid tumors

Anticancer oncolytic activity of respiratory syncytial virus

An endoplasmic reticulum-specific stress-activated caspase (caspase-12) is implicated in the apoptosis of A549 epithelial cells by respiratory syncytial virus

Syncytia formation in oncolytic virotherapy

Oncolytic virotherapy reverses the immunosuppressive tumor microenvironment and its potential in combination with immunotherapy

Oncolytic Zika virus promotes intratumoral T cell infiltration and improves immunotherapy efficacy in glioblastoma

Natural killer T cell immunotherapy combined with IL-15-expressing oncolytic virotherapy and PD-1 blockade mediates pancreatic tumor regression

Oncolytic adenoviruses synergistically enhance anti-PD-L1 and anti-CTLA-4 immunotherapy by modulating the tumour microenvironment in a 4T1 orthotopic mouse model. Cancer Gene Ther

Doxorubicin hydrochloride enhanced antitumour effect of CEA-regulated oncolytic virotherapy in live cancer cells and a mouse model

Immunostimulatory bacterial antigen-armed oncolytic measles virotherapy significantly increases the potency of anti-PD1 checkpoint therapy

Intratumoral expression of IL-7 and IL-12 using an oncolytic virus increases systemic sensitivity to immune checkpoint blockade

The oncolytic caprine herpesvirus 1 (CpHV-1) induces apoptosis and synergizes with cisplatin in mesothelioma cell lines: a new potential virotherapy approach

Combination treatment with oncolytic Vaccinia virus and cyclophosphamide results in synergistic antitumor effects in human lung adenocarcinoma bearing mice

Radiation therapy enhances the potential of the oncolytic virus in the treatment of osteosarcoma

Proceedings of the AACR Virtual Special Conference on Radiation Science and Medicine

Oncolytic adenovirus H101 synergizes with radiation in cervical cancer cells

Nanobugs as drugs: bacterial derived nanomagnets enhance tumor targeting and oncolytic activity of HSV-1 virus

Ursolic acid and its nanoparticles are potentiators of oncolytic measles virotherapy against breast cancer cells

Targeting programmed cell death-1 (PD-1) and ligand (PD-L1): a new era in cancer active immunotherapy

Virus-specific memory T cells populate tumors and can be repurposed for tumor immunotherapy

Adjuvant oncolytic virotherapy for personalized anti-cancer vaccination

An oncolytic adenovirus encoding SA-4-1BBL adjuvant fused to HPV-16 E7 antigen produces a specific antitumor effect in a cancer mouse model

Development and application of reverse genetic technology for the influenza virus

Optimal timing of PD-1 blockade in combination with oncolytic virus therapy

Parking CAR T cells in tumours: oncolytic viruses as valets or vandals? Cancers

DNA damage repair in glioblastoma: current perspectives on its role in tumour progression, treatment resistance and PIKKing potential therapeutic targets

Combination of mesenchymal stem cell-delivered oncolytic virus with prodrug activation increases efficacy and safety of colorectal cancer therapy

Replication and spread of oncolytic herpes simplex virus in solid tumors

The potential applications of stem cells for cancer treatment

Enhanced immunotherapeutic profile of oncolytic virus-based cancer vaccination using cyclophosphamide preconditioning

Ad-VT enhances the sensitivity of chemotherapy-resistant lung adenocarcinoma cells to gemcitabine and paclitaxel in vitro and in vivo

Induction of cell death in ovarian cancer cells by doxorubicin and oncolytic vaccinia virus is associated with CREB3L1 activation

Stereotactic body radiation combined with oncolytic vaccinia virus induces potent antitumor effect by triggering tumor cell necroptosis and DAMPs

The evolution and future of targeted cancer therapy: from nanoparticles, oncolytic viruses, and oncolytic bacteria to the treatment of solid tumors

Aptamer-based biosensors and application in tumor theranostics

Delivery of cancer therapies by synthetic and bio-inspired nanovectors

Chitosan-based nanoparticles for intracellular delivery of ISAV fusion protein cDNA into melanoma cells: a path to develop oncolytic anticancer therapies

ASO visual abstract: talimogene laherparepvec (T-VEC) for treatment of advanced locoregional melanoma after failure of immunotherapy: an international multi-institutional experience

Neoadjuvant talimogene laherparepvec plus surgery versus surgery alone for resectable stage IIIB-IVM1a melanoma: a randomized, open-label, phase 2 trial

Oncolytic herpesvirus therapy for mesothelioma --a phase I/IIa trial of intrapleural administration of HSV1716

Neural stem cell delivery of an oncolytic adenovirus in newly diagnosed malignant glioma: a first-in-human, phase 1, dose-escalation trial

Oncolytic adenovirus H101 synergizes with radiation in cervical cancer cells

Combination therapy of prostate cancer by oncolytic adenovirus harboring interleukin 24 and ionizing radiation

Phase I dose-escalation study of endoscopic intratumoral injection of OBP-301 (Telomelysin) with radiotherapy in oesophageal cancer patients unfit for standard treatments

The generation and analyses of a novel combination of recombinant adenovirus vaccines targeting three tumor antigens as an immunotherapeutic

Phase I study of a multitargeted recombinant Ad5 PSA/MUC-1/brachyury-based immunotherapy vaccine in patients with metastatic castrationresistant prostate cancer (mCRPC)

First-in-human study of TKpositive oncolytic vaccinia virus delivered by adipose stromal vascular fraction cells

Plasmacytoid dendritic cells orchestrate innate and adaptive anti-tumor immunity induced by oncolytic coxsackievirus A21

Image-guided radiovirotherapy for multiple myeloma using a recombinant measles virus expressing the thyroidal sodium iodide symporter

Oncolytic measles virus targets high CD46 expression on multiple myeloma cells

Oncolytic measles virus therapy enhances tumor antigen-specific T-cell responses in patients with multiple myeloma

Phase I trial of intravenous administration of PV701, an oncolytic virus, in patients with advanced solid cancers

Newcastle disease virus degrades SIRT3 via PINK1-PRKN-dependent mitophagy to reprogram energy metabolism in infected cells

Unleashing the therapeutic potential of oncolytic viruses

STAT3 contributes to oncolytic newcastle disease virus-induced immunogenic cell death in melanoma cells

Strong T-cell costimulation can reactivate tumor antigen-specific T cells in late-stage metastasized colorectal carcinoma patients: results from a phase I clinical study

Long-term remission of prostate cancer with extensive bone metastases upon immunoand virotherapy: a case report

Long-term survival of a breast cancer patient with extensive liver metastases upon immune and virotherapy: a case report

A phase I trial and viral clearance study of reovirus (Reolysin) in children with relapsed or refractory extra-cranial solid tumors: a Children's Oncology Group Phase I Consortium report

A phase I trial and viral clearance study of reovirus (Reolysin) in children with relapsed or refractory extra-cranial solid tumors: a Children's Oncology Group Phase I Consortium report

Effect of respiratory syncytial virus on the growth of hepatocellular carcinoma cell-lines

The oncolytic effect of respiratory syncytial virus (RSV) in human skin cancer cell line, A431. Iran Red

SARS-CoV-2-induced remission of Hodgkin lymphoma