key: cord-0694402-kzzgikk0
authors: Mou, Danlei; Feng, Huahua; Cao, Ruiyuan; Weng, Xing; Zhao, Lizhe; Yang, Lingling; Jin, Ronghua; Chen, Weijun
title: Profile of specific antibodies to the SARS-CoV-2
date: 2021-03-18
journal: J Med Microbiol
DOI: 10.1099/jmm.0.001335
sha: d7699dab397aadaf7afe8e704a016e4cd0052214
doc_id: 694402
cord_uid: kzzgikk0

In this work, we studied the profile of IgM and IgG antibody responses to SARS-CoV-2 in 32 patients with COVID-19 from day 1 to day 24. IgM remained measurable for a much shorter period than IgG, suggesting that IgG antibody may represent the primary immune response.

A novel coronavirus called the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), previously known by the provisional name 2019 novel coronavirus (2019-nCoV), has been identified as the causal agent of COVID-19 [1, 2] . SARS-CoV-2 is detectable in the blood, faeces and respiratory secretions of COVID-19 patients. Laboratory methods for SARS-CoV-2 diagnosis are primarily PCR-based to detect viral RNA in patient's secretions/excretions and antibodybased to detect their immune responses, such as the litres of IgM and IgG antibodies in the blood. To understand the humoral immunity to this virus, we studied the profile of IgM and IgG antibody responses to SARS-CoV-2, as well viral RNAs, in throat swabs and anal swabs from 32 COVID-19 patients.

In our study, IgM and IgG antibodies were analysed by an indirect ELISA in 32 patients with COVID-19 from day 1 of their illness to day 24 every other 4 days and then every other 10 days. The general criteria for enrollment of patients were as follows: epidemiological history, body fever and Ct value of SARS-CoV-2 in the throat swab is less than 38. By the guidelines made by the Chinese National Health Commission, patients were classified into three categories: mild (without pneumonia), moderate or severe. A severe COVID-19 case was defined as a case with any one of the following criteria: (1) respiratory rate is at or above 30 per minute; (2) fingertip oxygen saturation is at or below 93 % at resting state; or (3) the ratio of the partial pressure of oxygen (PaO2) to the fraction of inspired oxygen (FiO2) (PaO2: FiO2) is at or below 300 mmHg. By the time of randomization, moderate patients were within the first 7 days from the onset of symptoms, and severe patients were within the 14 days from the onset of symptoms. The information of 32 patients is shown in SARS-CoV-2 IgM/IgG ELISA Kit (GBI, Beijing, PR China, catalogue no. 0601046) was used for detection and the antigen is the full length of N protein expressed from Escherichia coli. The specificity of the ELISA kit is 99 %, and the clinical sensitivity depends on the course of the disease. The IgG sensitivity for patients after 2 weeks of illness is around 100 %. All 32 patients tested negative for IgM and IgG at day 4 after the onset of symptoms. Of these patients, 18 tested positive for IgM and 12 tested positive for IgG at day 8, 29 tested positive for IgM and 26 tested positive for IgG at day 12. (Fig. 1a) . All 32 patients were IgG-positive after day 16 and continued to have high levels of IgG up to 54 days after the onset of The specific antibodies to spike receptor binding domain (RBD) protein of SARS-CoV-2 were also tested using SARS-CoV-2 S1RBD IgG ELISA Kit (macro and micro-test, Jiangsu, PR China, catalogue no. 0601039), and the antigen was produced from insect cell. The specificity of the ELISA kit is 99 %, and the clinical sensitivity depends on the course of the disease. The sensitivity for patients after 2 weeks of illness is around 98 %. The IgG litres also peaked at 1.164±0.655 at day 24 and then declined (Fig. 1b) .

Viral RNA was also tested by the throat swabs and anal swabs of these patients using real-time fluorescent RT-PCR kit for detecting SARS-CoV-2 (BGI Biotechnology, Wuhan, PR China, catalogue no. RM0349), which were proved with sensitivity of 100 copies/ml and specificity higher than 99.9 % with ORF1ab as the target. It revealed that 30 tested positive at day 4, 25 tested positive at day 8, 18 tested positive at day 12, 6 tested positive at day 16, 1 tested positive at day 20 and then this patient still tests positive in anal swabs but negative in throat swabs after day 24 to 54.

Our results suggest that throat viral RNA tested has more sensitivity during the acute or early phase, but it has 6.25 % false negative at least. However, 100% of patients had antibody responses to SARS-CoV-2 during the convalescent phase. The SARS-CoV-2 specific IgM remained measurable for a much shorter period, but the IgG antibody persisted for a long time, suggesting that IgG antibody to SARS-CoV-2 may represent the primary humoral immune response protecting patients against COVID-19. IgG began to decline from around the fourth week and failed to stabilize at a high level. The decline of RBD antibody may be related to the neutralizing antibody titre, which indicates that there is a potential for reinfection in patients with SARS-CoV-2 infection for a period of time after recovery. The profile of antibodies against SARS-CoV-2 was consistent with the findings of SARS-CoV infection [3] . The presence of high litres of IgG antibody to SARS-CoV-2 spike RBD protein in the patients at the convalescent stage also suggests that a S protein vaccine for active immunization and a concentrated human SARS-CoV-2 specific IgG antibody for passive immunization could be developed for the treatment of COVID-19. The profile of anti-SARS-CoV-2 antibodies may be helpful in the diagnosis and in epidemiologic surveys.

The work was supported by the National Major Project for Control and Prevention of Infectious Disease in China (2018ZX10732-401, 2018ZX10301101-004).

Dr Mou is the Chief physician for Beijing Youan Hospital, Capital Medical University, Beijing, China. Her primary research interests are investigation and diagnosis of viral hepatitis, fever and rash, acquired immunodeficiency syndrome and viral hemorrhagichaemorrhagic fever.

A novel coronavirus from patients with pneumonia in China

Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding

Profile of specific antibodies to the SARSassociated coronavirus

The authors declare that there are no conflicts of interest.

This study and all the relevant experiments were approved by the Beijing Youan Hospital Research Ethics Committee, and written informed consent was obtained from each participant in accordance with the Declaration of Helsinki.

Five reasons to publish your next article with a Microbiology Society journal 1 . The Microbiology Society is a not-for-profit organization. 2. We offer fast and rigorous peer review -average time to first decision is 4-6 weeks. 3. Our journals have a global readership with subscriptions held in research institutions around the world. 4. 80% of our authors rate our submission process as 'excellent' or 'very good'. 5. Your article will be published on an interactive journal platform with advanced metrics.Find out more and submit your article at microbiologyresearch.org.