key: cord-0691082-pkok7os5
authors: Hayashi, Kumiko; Niwa, Shinsuke
title: Japan-US symposium on cytoskeletal motor proteins and their associated proteins
date: 2021-10-06
journal: Biophys Physicobiol
DOI: 10.2142/biophysico.bppb-v18.026
sha: f3b271e8f7e5c2e71694386352f46f88cdad0a61
doc_id: 691082
cord_uid: pkok7os5

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imaging to better understand organelle transport in neurons." See details of the Holzbaur lab [4] and a recent publication [5] .

Dr. Kristen Verhey (University of Michigan) will talk about a rogue kinesin that destroys microtubules in cells. She describes the breakthroughs made in her laboratory by saying "Recent work on kinesins has raised the possibility that the physical act of motors walking on microtubules creates stress in the microtubule lattice. Whether processive motility of kinesin and/or dynein motors creates stress and/or defects in the microtubule lattice in cells has not been determined. Here, we describe a kinesin-1 mutant that causes microtubule destruction when expressed in cells. Using in vitro assays, we show that the mutant motor is unable to promote rescues in microtubule dynamics assays and generates large defects in the microtubule lattice in microtubule repair assays. These findings suggest that the mutant is an unnatural or rogue motor whose activity would have been selected against during evolution." See the details of the Verhey lab [6] and a recent publication [7] .

From the Japanese perspective, recent studies from three laboratories have been introduced. Dr. Ryohei Kobayashi from the Hiroyuki Noji lab [8] discusses the single-molecule experiment on IF 1 , a regulatory protein of mitochondrial ATP synthase [9] ; Dr. Jakia Jannet Keya from the Ryota Iino lab [10] talks about engineering hybrid kinesin with a synthetic linker [11] ; and Dr. Shinsuke Niwa, sharing the talk with Dr. Kyoko Chiba from the Shinsuke Niwa lab [12], provides topics on genetics using C. elegans and single-molecule experiments with several types of kinesin [13, 14] . Dr. Kumiko Hayashi [15] , who is a member of both the Biophysical Society [16] and the Biophsyical Society of Japan [1] , is the moderator and overall host of the joint symposium, promoting debates from an interdisciplinary point of view. We hope that the joint symposium will promote the exchanges of ideas between the research communities of Japan and the USA. 

Optical control of fast and processive engineered myosins in vitro and in living cells

Mitochondrial dynamics: Shaping and remodeling an organelle network

Pathogenic mutations in the kinesin-3 motor KIF1A diminish force generation and movement through allosteric mechanisms

Kinetic analysis of the inhibition mechanism of bovine mitochondrial F 1 -ATPase inhibitory protein using biochemical assay

Introduction: Molecular Motors

De novo disease-associated mutations in KIF1A dominant negatively inhibit axonal transport of synaptic vesicle precursors

Reconstitution of Kinesin-1 Activation. bioRxiv

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