key: cord-0690545-50rmr19x
authors: Yang, Kexun; Wang, Qinzhang
title: 50 week ultrasound imaging and ultrastructural abnormalities of bladder after sugar diuresis and diabetes mellitus in rats
date: 2021-06-10
journal: Int Urol Nephrol
DOI: 10.1007/s11255-021-02911-w
sha: 2f7d579e2e225fcc048f5f3f385fcce397238372
doc_id: 690545
cord_uid: 50rmr19x

PURPOSE: To investigate 50 week ultrasound imaging and ultrastructure changes of bladder in diuresis and diabetes rats. METHODS: Forty-two healthy male Sprague–Dawley rats were randomly divided into control group, sugar-induced diuresis group and streptozotocin-induced diabetes group. The 24 h drinking and urine volume were calculated from 21 to 31 weeks. Using ultrasound to assess bladders after 49 weeks. Bladders were examined by transmission electron microscope after 50 weeks. RESULTS: The drinking and urine volume significantly increased in the diuresis and diabetes groups. The bladder morphology and bladder wall thickness increased in the diuresis and diabetes groups. Bladder stones, bladder overdistension and urinary retention were seen in the diuresis and diabetes groups. Urothelium manifested degeneration, denudation and necrosis in the diuresis and diabetes groups. The mitochondrial vacuolar degeneration in the urothelial cells was seen in the diabetes group. The subepithelial vascular endothelial cells hyperplasia with a narrowed lumen were observed in the diabetes group. Abnormal mitochondria were rarely seen in the control group. The mitochondrial vacuolar degeneration in the detrusor was more severe in the diabetes group than in the diuresis group. The detrusor muscle and axon degeneration were observed in the diuresis and diabetes groups. Two rats in the diuresis group share similarities with diabetes group (2/6). CONCLUSION: Long-term diabetes mellitus can cause increments of urinary bladder morphology and bladder wall thickness, urinary retention and bladder stones. Ultrastructural degeneration of the bladder might be the morphological bases of diabetic cystopathy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11255-021-02911-w.

Diabetic cystopathy (DCP) is a common complication of diabetes mellitus (DM). The urodynamic findings include reduced bladder sensation, increased cystometric bladder capacity, decreased bladder contractility, impaired uroflow, and increased residual urine volume later [1] . However, the pathogenesis of DCP has not been fully elucidated.

Previous studies have revealed the ultrastructure changes in neurogenic and non-neurogenic bladder dysfunction and compared with the urodynamic findings [2] [3] [4] . The ultrastructure abnormalities may explain the lower urinary tract symptoms. Pathological research of DCP is limited [5, 6] .

Impact of persistent and chronic hyperglycemia induced urinary bladder dysfunction is poorly understood.

Ultrasound imaging has become increasingly important in the assessment of lower urinary tract dysfunction. Using ultrasound to assess bladders of diabetic animals has not been conducted before.

The characteristic of polydipsia and polyuria with DM may influence the function of the urinary bladder in some degree [7] . We use 5% white granulated sugar-induced osmotic diuresis (OD) to imitate polydipsia and polyuria.

The objective of this study is to investigate the long-term (50 weeks) ultrasound imaging and ultrastructure abnormalities of bladder after sugar diuresis and diabetes mellitus in rats, providing morphological bases of DCP.

Adult male Sprague-Dawley rats (8 weeks old, Xinjiang Medical University Laboratories, Urumchi, China) were randomly divided into control group (n = 12), OD group (n = 12) and DM group (n = 18). Diabetes mellitus was induced by a single intraperitoneal injection of 50 mg/kg body weight of streptozotocin (STZ, Sigma, USA) dissolved in a citrate buffer (0.1 M, pH 4.5). Animals with blood glucose higher than 16.7 mmol/L with an automatic glucometer (Accu-Chek ® , Roche, Germany) 72 h after STZ injection were classified as diabetic [7] . Osmotic diuresis was induced by adding 5% white granulated sugar to the rats' daily drinking water. The diuresis and age-matched control rats were injected with a proportionate volume of buffer alone. Blood glucose was measured weekly. To prevent excessive hyperglycemia and death, DM rats received three units of NPH human insulin (Lilly, France) injection if the blood glucose was higher than 33.3 mmol/L (HI was displayed on the glucometer). The experimental protocol was approved by the first affiliated hospital of Shihezi University Institutional Animal Care and Use Committee.

From 21 to 31 weeks, the rats were placed in metabolic cages. The animals were housed in groups of two per cage. A known volume of water or 5% sugar was placed in the drinking bottles. Urine was collected by clean plastic cups. At the end of 24 h, the remaining volume in the drinking bottles and voiding volumes were measured, and the consumed volume was calculated.

Skin preparation and water deprivation were performed before measurement. The probe of the clinical ultrasound machine (Hitachi, EZU-MT28-S1,12 MHz linear probe; Tokyo, Japan) was placed over the suprapubic area in awake rats.

Empty bladder was ensured by four steps. First, water deprivation for more than 3 h. Second, the operator applied a gentle force to the suprapubic area to promote micturition before measurement [8] . Then, the sonographer used the ultrasonic probe to apply pressure to the suprapubic area and verify the filling and emptying state of the bladder. Finally, if PVR existed, the operator repeated step two and three at least once [9] . As for diabetic rats, if PVR were still existence, urinary retention was considered.

When there was no discharge of urine after the above operation, ultrasound acquisitions were quickly performed considering empty bladder. Images of bladders were recorded in both sagittal and transverse plane. The greatest transverse (width), anterioposterior (depth), and superioinferior (height) distances and bladder wall thickness (BWT) were recorded. The BWT was measured on the transverse plane. We measured at least eight positions on the ventral and dorsal side on the images using ImageJ (1.53c). And means were calculated for analyzing the BWT. The ultrasound was performed by the same sonographer.

Animals were euthanized by excess pentobarbital anesthesia at 50 weeks (n = 6, control; n = 6, OD; n = 6, DM). The dome of bladder was quickly sliced into 1 mm 3 . The samples were immersed in 2.5% glutaraldehyde solution for 48 h and further fixed in 1% osmium tetroxide for 1.5 h. Tissues were dehydrated by an acetone series and embedded in epoxy resin. Ultrathin sections were stained with uranyl acetate and lead citrate. The samples were viewed with the JEOL 1200 TEM (JEOL Ltd., Akishima, Tokyo, Japan).

Changes in the urothelium and detrusor smooth muscle were recorded. The quantification of the different stages of mitochondrial vacuolar degeneration in the detrusor used the methods described by Chaanine [10] and Lu et al. [4] Each mitochondrion was evaluated as 0-5 to represent the severity of structural destruction. Additional three photographs of different fields of detrusor were obtained randomly in each specimen in 12,000× magnification. Table 1 General characteristics a Statistical significance for the DM group compared to the control group (P = 0.017). Statistical significance for the DM group compared to the control and OD group (P = 0.025) b Statistical significance for the control group compared to the OD group (P = 0.022). Statistical significance for the control group compared to the DM group (P < 0.001) c Statistical significance for the control group compared to the OD group (P < 0.001). Statistical significance for the control group compared to the DM group (P < 0.001) d Statistical significance for the control group compared to the OD group (P < 0.001). Statistical significance for the control group compared to the DM group (P < 0.001) 

The results are expressed as the mean ± standard deviation. The quantitative data performed parametric test. Comparisons among three groups were performed using oneway analysis of variance followed by Dunnett's t test or

Kruskal-Wallis H test followed by Nemenyi test. Statistical analysis was performed using SPSS 26.0 (IBM Corp., NY, USA). P < 0.05 was considered statistically significant. H The sphincter was closed after micturition. Compared to the control rats, urinary retention was observed

The remaining rats in DM, OD and control were 8, 9 and 11, respectively (due to the COVID-19) up to 49 weeks. All the DM rats showed cataracts except one. The necrosis of tails was only observed in the DM rats. During 21-31 weeks, the DM group manifested weight loss, polydipsia and polyuria (nearly 4.3 times and 10.8 times compared to the control group, Table 1 ).

The bladder morphology and BWT increased in awake OD and DM rats (Fig. 1 , Table 2 ). Bladder overdistension, urinary retention and bladder stones were noticed in OD and DM groups (Fig. 2) . A diabetic rat showed bladder overdistension and micturated due to ultrasonic probe pressure. Urinary retention was observed after micturition (Fig. 1G , H). The bladder stone was invisible on the ultrasound imaging in the OD rat and accompanied by prostatic hyperplasia. The stone composition in the DM rat was calcium carbonate (Infrared Spectroscopy Analysis System, type LIIR-20, Tianjin). 

The urothelium degeneration, exfoliation and necrosis were observed in the OD and DM groups (Fig. 3 , Table 3 ). An OD rat with hyperglycemia showed severe mitochondrial vacuolar degeneration in the urothelium. An OD rat with hyperglycemia showed steatosis of urothelial cells. In the DM group, the mitochondrial vacuolar degeneration in the urothelium was prominent. The endoplasmic reticulum was swollen in some cases. Cell junctions between adjacent umbrella cells were decreased. The subepithelial vascular endothelial cells hyperplasia with a narrowed lumen (Fig. 4) were observed in the DM group. The autophagosomes were observed both in the OD and DM groups (Fig. 5) . 

Rodlike mitochondria in the control group were widespread. However, globular mitochondria were seen in the DM group. The mitochondrial vacuolar degeneration in the detrusor was more severe in the DM group than the OD group (Figs. 6 and 7, Table 3 ). Abnormal mitochondria were rarely seen in the control group. The OD and DM groups showed degenerating muscle cells with disrupted sarcolemma, myofilaments and vacuolar degeneration, and cell junctions reduced with depleted caveolae. Flocculent degeneration was seen in the sarcoplasm and axoplasm of the DM rats. Local severe degeneration with indiscernible detrusor profile linked by protrusion junctions was observed in the DM rat. Distinctive protrusion junctions were observed in the DM rats. Neuroeffector junction with muscle cells were indiscernible and intrinsic nerves of detrusor degeneration were seen. 3 DM rats showed unilateral kidney tumor. One of them measured nearly 1.9 cm × 1.8 cm, invading the pelvicalyceal system, perirenal and renal sinus fat but not beyond Gerota's fascia. Histopathology was renal cell carcinoma (Fig. 8 ).

Previously studies focused on the functional changes of DCP. Herein, we try to provide the histological appearance of DCP. As for the animal models, the longest time of previous research on diabetic rats was approximately 20 weeks. However, DM and its complications are always persistent and chronic.

Ultrasound demonstrated the morphology and BWT increased in the OD and DM groups. Combined with polydipsia and polyuria, bladder overdistension and urinary retention, the morphological changes may be a compensational adaptive response to diuresis and incomplete bladder emptying.

Little attention has been given to the ultrastructural changes of DM on the urinary bladder. Mitchell et al. reported the necrosis and desquamation of the umbrella cells in diabetic rats at 9 weeks, while the urothelium recovered at 20 weeks [6] . They attributed it to the burden of chronic hyperglycemia and adaptive response. Rizk et al. did not mention this phenomenon in diabetic rats at 6, 10 and 16 weeks [5] . However, the degeneration, necrosis and exfoliation of the urothelium still existed both in the OD and DM groups after 50 weeks. In some dead diabetic rats, the microorganism could be observed under microscopy. While we did not find obvious microorganisms under the TEM at 50 weeks. The urothelium is a barrier to urine. The urothelium exfoliation could also be seen in cyclophosphamide chemotherapy, BCG immunotherapy and radiotherapy. Therefore, they may be a response to injurious stimulus.

Lu et al. have performed morphometry of human detrusor mitochondria with urodynamic after partial bladder outlet obstruction [4] . They found that the mitochondrial damage score correlated with the linearized passive urethral resistance relationship score. The mitochondria of the vesical urothelium, subepithelial vasculature, detrusor muscle and axon manifested vacuolar degeneration in the DM.

The flocculent degeneration in the sarcoplasm and axoplasm was reported in unilateral sacral ventral rhizotomy in the cats [11] . This may indicate that the degeneration of detrusor muscle was associated with axon degeneration. Protrusion junctions were proposed as a manifestation of muscle cell de-differentiation associated with detrusor overactivity [12] . The detrusor and axon degeneration may explain incomplete bladder emptying. Neuromuscular dysfunction of bladder may be the structural bases of the detrusor overactivity and acontractile detrusor. And the bladder stone might be associated with chronic hyperglycemia, glycosuria, increased urine calcium excretion [13] and neuromuscular dysfunction [14] .

Sugar-induced osmotic diuresis was often used to mimic the symptom of polyuria and polydipsia with DM. Liu et al. found time-dependent decrease of density of the nerves and vasculatures in the bladder tissues both in the diuresis and diabetes group. They concluded diabetes and diabetesinduced polyuria contributed to the alteration [15] . However long-term extra sugar intakes may cause impaired fasting Fig. 5 Autophagosomes in the urothelium A OD group, the autophagosome consists of double-membrane. B DM group, the autophagosome was adjacent to the lysosome glucose, impaired glucose tolerance and type 2 DM (T2DM) [16] . T2DM-induced bladder ultrastructural changes share similar manifestations with STZ-induced DM.

Hyperglycemia induced oxidative stress causing diabetic complications. Gene expression for oxidative stress in diabetic animals demonstrated a significant increase and a downstream effect on protein damage and apoptosis [17] . In diabetes, oxidative phosphorylation fails, leading to decreased ATP production and increased reactive oxygen species levels, which causes mitochondrial dysfunction and oxidative damage of urothelium, subepithelial vasculature, detrusor muscle and axons [18] .

STZ-induced kidney tumors were first reported by Arison et al. [19] . WHO listed STZ as a cancerogen (group 2B) [20] . Dombrowski et al. reported nephrocarcinogenesis in spontaneously diabetic rats (no STZ injection) [21] . A previous study found that type 1 diabetes mellitus increased carcinogenic hazards to humans including kidney neoplasms [22] . Future studies need to research the mechanism of diabetes mellitus caused renal cell carcinoma.

Our study has some limitations. First, we could not collect the rat urine to analyze and build the association between urinary infections and the desquamation of the urothelium. The urine in the plastic cup was contaminated after 24 h. After anesthesia, there was not enough urine volume in the control rats (less than minimum requirement). Second, although we measured the BWT at an empty bladder depending the actual situation, a full bladder would be more accurate. However, this condition need anesthesia and diabetic rats may be intolerant. Even if a specified amount of water (for example 1.5 mL) were injecting into the bladder, ultrasonic probe pressure can promote micturition during measurement based on our experience and a former study [23] . Transurethral bladder catheterization can cause cystitis ( Supplementary Fig. 1 ) and influence the ultrastructural results in this study.

Long-term diabetes mellitus can cause increments of urinary bladder morphology and bladder wall thickness, urinary retention and bladder stones. The histological degeneration of the urothelium, subepithelial vasculature, detrusor muscle and axon might be the pathological bases of DCP. Fig. 8 Kidney neoplasm tumor cell with distinct cell membranes and optically clear cytoplasm. They are arranged in sheets, compact nests, alveolar, acinar and microcystic or even macrocystic structures, separated by an abundance of thin wall blood vessels (×200)

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