key: cord-0686705-wkbsb6gu
authors: Lundstrom, Kenneth
title: Coronavirus pandemic: treatment and future prevention
date: 2020-11-03
journal: Future microbiology
DOI: 10.2217/fmb-2020-0174
sha: bcb03d84d89ba5970d8ddac16770046d725906c3
doc_id: 686705
cord_uid: wkbsb6gu

The rapid spread of SARS-CoV-2 leading to the COVID-19 pandemic with more than 400,000 deaths worldwide and the global economy shut down has substantially accelerated the research and development of novel and efficient COVID-19 antiviral drugs and vaccines. In the short term, antiviral and other drugs have been subjected to repurposing against COVID-19 demonstrating some success, but some excessively hasty conclusions drawn from significantly suboptimal clinical evaluations have provided false hope. On the other hand, more than 300 potential therapies and at least 150 vaccine studies are in progress at various stages of preclinical or clinical research. The aim here is to provide a timely update of the development, which, due to the intense activities, moves forward with unprecedented speed.

been tested positive for COVID-19 and at least 1 million deaths have been recorded as of 13 October 2020 [14] . Independent of whether the pandemic will further expand, it will die out or return in a seasonal pattern, there is an urgent need for diagnostics to identify carriers and persons, who have recovered from COVID-19 [15] . Moreover, there is an acute need for developing new drugs against COVID-19. However, efficient vaccines against COVID-19 are absolutely essential for allowing life on the planet to return to what could be considered as normal conditions. In this review, the potential targets for antiviral drugs and vaccine development are described. Moreover, the current situation related to repurposing and novel drugs is summarized. Finally, an update on vaccine development is presented including the current status on preclinical and clinical studies.

Certain stages of the lifecycle of coronaviruses, particularly the virus attachment and entry (Figure 1 ), provide potential targets for antiviral drug and vaccine development against SARS-CoV-2 [16] . The ssRNA genome of SARS-CoV-2 is encapsulated by the structural spike S, envelope E, membrane M and nucleocapsid N proteins [17] . The initial attachment of SARS-CoV-2 takes place between the RBD of the S1 region of S the protein and its host cell receptor. This receptor interaction is of great importance as it defines the virus tropism as different coronaviruses target different host cell receptors. For instance, MERS-CoV recognizes DPP4 [18] , while both SARS-CoV and SARS-CoV-2 target ACE2 [19] . For this reason, ACE2 has been selected as a drug target for the development of angiotensin receptor blockers, monoclonal antibodies and even plant and mushroom extracts based on traditional Chinese medicine [20] . Replication of viral ssRNA in the cytoplasm presents another important target for antiviral drug development and several drugs acting on RdRp have been demonstrated to decrease viral RNA production [21] . Moreover, RNAi-based gene silencing is another approach to inhibit viral replication [22] . Similarly, vaccine development relies strongly on efficient target identification independent on whether the approach involves subunit or peptide vaccines or vaccines based on viral vector or nucleic acid delivery. The identification of approaches for production of antigens, which can elicit strong neutralizing immune responses and potentially provide protection against challenges with viral pathogens is essential. In the context of drug design, bioinformatics can play an important role. In the case of vaccine development, immuno-informatics can additionally be of great support. 

The urgent need for drugs against COVID-19 has encouraged investigators to verify the potential of repurposing drugs, previously developed as antiviral drugs for other viral or other infectious diseases. For instance, lopinavirritonavir has been used for treatment and prevention of HIV/AIDS [23] , remdesvir was designed for hepatitis C and Ebola virus disease therapy [24] and hydroxychloroquine as an antimalarial agent [25] . Recently, in search of additional repurposing drug candidates, 26 SARS-CoV-2 proteins expressed in human cells showed protein-protein interaction with 332 human proteins by affinity-purification mass spectrometry [26] . The study further revealed 69 ligands, which included preclinical and clinical compounds as well as US FDA-approved drugs, of which at least five targets and more than ten different chemotypes could present potential antiviral drug targets for COVID-19. Potential repurposing drugs for COVID-19 therapy are summarized and listed in Table 1 .

The combination of lopinavir/ritonavir has been demonstrated to inhibit HIV protease activity and has previously been successfully used for the treatment of HIV/AIDS [52] . Related to coronaviruses, marmosets with a MERSlike disease were subjected to lopinavir/ritonavir treatment, which resulted in improved clinical, radiological and pathological outcomes and reduction in viral load [27] . Furthermore, a multicentre, placebo-controlled, doubleblind randomized clinical trial for lopinavir/ritonavir combination therapy is ongoing for hospitalized patients with laboratory-confirmed MERS [28] . Related to SARS, a clinical study in 41 patients treated with lopinavir/ritonavir and ribavirin presented favorable clinical responses compared with ribavirin treatment alone [29] .

In the context of COVID-19, 99 patients receiving lopinavir/ritonavir showed no difference in clinical improvement, mortality or detectable viral RNA levels in comparison to control patients subjected to standard of care [30] . In a single-blind, randomized clinical trial, 44 patients with mild or moderate manifestation of COVID-19 received lopinavir/ritonavir or arbidol [31] . In general, no differences were seen for pharyngeal SARS-CoV detection, pyrexia, cough or lung CT scans between the treatment and control groups although the percentage of patients with severe or critical status was higher after lopinavir/ritonavir treatment (38.1%) compared with 12.55 for arbidol and 14.3% for control groups. Based on 143 publications, lopinavir/ritonavir showed no clear benefit compared with standard care [53] . Although there was some reduction in acute distress syndrome, the benefit-risk profile for lopinavir/ritonavir cannot be considered positive for treatment of COVID-19 patients.

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The nucleoside analogue favipiravir is a potent inhibitor of viral RNA polymerase showing efficacy against a wide range of influenza viruses [54] . Patients with laboratory-confirmed COVID-19 received either favipiravir or lopinavir/ritonavir in addition to IFN-α [32] . The results demonstrated a shorter viral clearance and a significant improvement in chest imaging for patients treated with favipiravir. Very recently, it was announced that Glenmark Pharmaceuticals will start a Phase III clinical trial in India, where 150 patients with mild to moderate COVID-19 will be enrolled [33] .

Much attention has been paid to the use of the adenosine nucleoside triphosphate analogue remdesivir for COVID-19. For instance, compassionate treatment of 53 COVID-19 patients resulted in clinical improvement in 36 patients (68%), discharge of 25 patients and death of seven patients [34] . In another study in China, 158 patients received remdesivir and 79 patients were subjected to placebo, which demonstrated no statistically significant benefits of remdesivir treatment over placebo, although the time was reduced for clinical improvement to occur [35] . Moreover, in a double-blind, randomized, placebo-controlled trial in 1059 adult COVID-19 patients, remdesivir showed shorter patient recovery time (11 days) compared with placebo (15 days) [36] . The Kaplan-Meier estimates of mortality was also lower for patients receiving remdesivir (7.1%) than placebo (11.9%). Next, an additional 5600 patients will be enrolled in the trial, which will be conducted in China, France, Germany, Hong Kong, Italy, Japan, Korea, The Netherlands, Singapore, Spain, Sweden, Switzerland, Taiwan, the UK and the USA.

Chloroquine and hydroxychloroquine with at least 80 registered clinical trials have recently received much attention as potential therapeutics against COVID-19, partly based on ill-founded political over-optimistic statements and on claims from badly designed and executed clinical trials [20] . Although a study on 20 COVID-19 patients in France demonstrated reduction in viral load, the trial design was poor and the results were unreliable with six patients dropping out [37] . In an open label randomized controlled study in 150 hospitalized COVID-19 patients in China, hydroxychloroquine administration did not show any advantage to standard of care treatment alone [38] . In contrast, the study indicated that hydroxychloroquine decreased the survival of hospitalized patients and increased the risk of ventricular arrhythmias. Administration of chloroquine and hydroxychloroquine has also been associated with adverse reactions in patients [55] . For instance, high dose chloroquine caused more severe delayed ventricular repolarisation, QT prolongation, in COVID-19 patients in Brazil [56] . Moreover, patients treated with hydroxychloroquine and azithromycin showed statistically significant changes in QT prolongation suggesting a high risk for arrhythmia [57] .

Camostat mesylate, a serine protease inhibitor has been used for pancreatitis and cancer treatment [39] . As camostat mesylate acts as an inhibitor of transmembrane protease serine 2, it was demonstrated to block the spread of SARS-CoV in a mouse model by prevention of the interaction with the CoV S protein [40] . More recently, it was shown that camostat mesylate blocked the entry into lung cells of SARS-CoV-2 isolated from a patient [41, 58] . Previously, camostat mesylate has shown very few adverse events and the use of the drug for the treatment of acute symptoms of chronic pancreatitis in more than 100,000 individuals in Japan, only one case of acute eosinophilic pneumonia was reported [59] . Currently, an estimated 114 individuals will be recruited for a double-blind randomized controlled Phase II clinical trial comparing camostat mesylate treatment to placebo in COVID-19 patients [60] .

Repurposing sofosbuvir, an anti-HCV antiviral agent, has been based on the high sequence and structural homology of the RdRps of HCV and SARS-CoV-2 [61] . In silico modelling suggests that sofosbuvir can tightly bind to the SARS-CoV-2 RdRp leading to viral eradication. Moreover, sofosbuvir is safe, well tolerated and shows high intracellular stability and might therefore be suitable for clinical trials for COVID-19 patients [42] .

Finally, as natural products have demonstrated inhibitory effect on viral infection and replication, they might also be adequate for the treatment of COVID-19 [62] . Several flavonoids interfere with activation of the NLRP3 inflammasome showing activity against enteroviruses [63] and Dengue virus [43] . Moreover, the anthraquinone compound emodin produced by many fungi species and found in Chinese herbs has been demonstrated to inhibit the interaction of SARS-CoV S protein with ACE2 [44] . The polyphenol resveratrol, found in high concentrations in grapes, red wine and sprouted peanuts has been shown to inhibit MERS-CoV infection in vitro and might also be effective against SARS-CoV-2 [45, 64] .

In addition to efforts to evaluate antiviral drugs developed for other indications, intense activity in development of novel drugs is in progress (Table 1 ). In this context, the x-ray structure of the SARS-CoV-2 3CL pro protease alone or complexed with α-ketoamides allowed the design of specific 3CL pro inhibitors with favorable pharmacokinetic Review shRNAs for ACE2 SARS Reduced SARS-CoV infection in Vero cells [80] miRNAs for MERS-CoV ORF1ab MERS Computational predictions for MERS silencing [81] siRNAs for MERS-CoV ORF1ab MERS Computational predictions for MERS silencing [81] shRNAs for M, N PDCoV Decrease in viral titres and RNA levels in ST cells [82] shRNAs for M PEDV Inhibition of viral RNA replication [22] shRNAs for M SADV Inhibition of viral RNA replication [22] siRNAs for ORF1ab, 3a, s, M, N COVID-19

Computational design of SARS-CoV-2 siRNAs [83] properties in mice [46] . In another study, the N3 inhibitor of 3CL pro was identified by combined structureassisted drug design, virtual drug screening and high-throughput screening computer-aided drug design [65] . The N3 inhibitor demonstrated irreversible inhibition of SARS-CoV-2 3CL pro . Additional high-throughput screening identified ebselen, which showed anti-inflammatory, antioxidant and cytoprotective properties in SARS-CoV-2infected Vero cells. Obviously for blocking virus entry, ACE2 is a relevant drug target for COVID-19, providing the advantage of targeting the host ACE2 protein and not allowing the virus to circumvent the drug activity by mutations [47] . One approach is to target the small RBD in the S protein, which has been indicated as the key domain for binding ACE2 and it has demonstrated efficient inhibition of entry of SARS-CoV in cell cultures [48] . The same strategy can be applied for the equivalent RBD for SARS-CoV-2. An alternative approach was applied for MERS-CoV, where an Fc fragment was attached to the RBD protein to extend its circulation time and to block viral infection in mice [66] . However, this strategy requires the elimination of cytotoxic Fc domain functions, since the RBD-Fc fusion can also bind to normal cells [67] . A second similar strategy, already demonstrated for SARS-CoV entry and replication [68] , relates to the administration of an antibody that binds to the ACE2 protein. Another option is to utilize a nanobody or VHH domains from camelids [69, 70] . Importantly, although the ACE2 binding affinity is similar for SARS-CoV and SARS-CoV-2, the furin cleavage site uniquely present in the SARS-CoV-2 S protein provides the means for designing specific SARS-CoV-2 inhibitors [49] . In the context of therapeutic monoclonal antibodies, the first SARS-CoV-2-specific human monoclonal antibody CR3022 demonstrated potent binding to the RBD of SARS-CoV-2 S [50] . However, as CR3022 does not overlap the ACE2 binding site, it might need to be combined with other neutralizing antibodies. Moreover, the need for monoclonal antibodies with specific binding affinity to the SARS-CoV-2 RBD was highlighted by the finding that the potent SARS-CoV-specific m396 and CR3014 neutralizing antibodies did not show binding or the SARS-CoV-2 S protein. Recently, the human 47D11 monoclonal antibody targeting a communal epitope was demonstrated to neutralize both SARS-CoV and SARS-CoV-2 in cell cultures and may potentially offer prevention and treatment of COVID-19 [71] .

Another approach, which also could be described as a repurposing drug relates to existing AT1R blockers such as losartan, successfully used for hypertension treatment [51] . AT1R is a valid target as ACE2, which activates AT1R by cleavage of angiotensin I, serves as the binding site for both SARS-CoV and SARS-CoV-2 [72] . A rapid approach would therefore be to investigate whether patients subjected to AT1R antagonist treatment due to hypertension, diabetic kidney disease or other indications have a better disease outcome or a lower frequency of hospitalization than the general population.

Gene silencing based on RNAi has been used for basic research for years [73] but represents a fairly novel approach for treatment of viral diseases [74] . Briefly, RNA molecules can be engineered as siRNAs, shRNAs and miRNAs in the form of 19-23 bp dsRNAs mediating sequence-specific degradation of target mRNA [75] . In the context of coronaviruses, gene silencing has been applied for siRNAs to inhibit SARS-CoV replication in Vero E6 cells (Table 2 ) [76] . In another study, 48 siRNA sequences covering the SARS-CoV genome were engineered [77] . Transfection of chemically synthesized siRNAs into fetal kidney cells, before or after SARS-CoV infection, resulted in four siRNAs showing potent inhibition of infection and replication. The prophylactic effect of 90% lasted for at least 72 h. Combination of siRNA duplexes also significantly suppressed SARS-like symptoms in rhesus macaques [78] .

future science group www.futuremedicine.com mRNA-based delivery of SARS-CoV-2 sequences High levels of neutralizing antibodies after immunization with 2 μg of mRNA CureVac [99] saRNA-based delivery of SARS-CoV-2 sequences Preclinical evaluation in progress Imperial College London [100] Furthermore, siRNA-silenced ACE2-targeted expression in Vero cells, which also led to reduced SARS-CoV infection [80] . Similarly, siRNA duplexes knocked down expression of the actin-binding protein ezrin, which is known to interact with the SARS-CoV S protein during virus entry [79] . Related to MERS-CoV, four miRNA and five siRNA molecules from the ORF1ab region were rationally designed by computational methods for the silencing of nine MERS-CoV strains [81] . The potency of the in silico designed RNAi molecules needs next to be verified for MERS-CoV inhibition in cell lines and in vivo. In another study, shRNAs targeting the porcine delta coronavirus (PDCoV) M and N genes showed 13.2-and 32.4-fold reduction, respectively, in titres when swine testicular cells were challenged with PDCoV [82] . Likewise, the viral RNA decreased by 45.8 and 56.1%, respectively. Moreover, shRNAs targeting the M gene of porcine epidemic diarrhea virus (PEDV) and swine acute diarrhea virus and the N gene of PDCoV, expression of each viral RNA was inhibited more than 98% [22] . Additionally, the viral replication was significantly impaired for porcine epidemic diarrhea virus, SADS-CoV and PDCoV. In the case of COVID-19, computational strategies have identified nine siRNAs targeting ORF1ab, ORF3a, S, M and N sequences, which should next be evaluated in cell lines and in vivo [83, 84] .

The struggle with developing novel or repurposed antiviral drugs against COVID-19 has further strengthened the demand for the need of efficient vaccines against the current SARS-CoV-2, its potential mutated versions and other emerging viruses [20] . Needless to say, there are numerous vaccine development efforts in progress today with more than 100 vaccine candidates at the preclinical stage and at least 13 vaccine candidates in clinical trials [85, 86] . The spectrum of approaches is broad comprising live attenuated virus, inactivated virus, protein subunits, nonreplicating and replicating viral vectors and nucleic acid vaccines based on DNA plasmids, mRNA molecules and self-replicating RNA vectors (Table 3) . Although numerous studies have been conducted on other animal and human coronavirus vaccines as previously described [20] , the focus here is uniquely on COVID-19 vaccines.

In the context of live-attenuated vaccines, viral deoptimization has been used for the rapid generation of multiple SARS-CoV-2 vaccine candidate genomes for preclinical testing [87] . The vaccine production will then be subjected to scale-up manufacturing and clinical evaluation. Related to inactivated COVID-19 vaccines, the BBIBP-CoV vaccine candidate elicited neutralizing antibodies in mice, rats, guinea pigs, rabbits and nonhuman primates [88] . Vaccination with two doses of 2 μg provided protection against SARS-CoV-2 in rhesus macaques and the good genetic stability for vaccine manufacturing will allow evaluation in clinical trials. Several preclinical studies on protein subunit vaccines are in progress utilizing nanoparticles, virus-like particles, SARS-CoV S spike protein with or without adjuvant, various peptides and molecular clamp technologies [85] . Application of molecular clamp technologies has allowed to lock unstable prefusion versions of surface proteins in a form that shows better immunogenicity [101] and subjected to preclinical studies for the SARS-CoV-2 S protein has demonstrated high levels of neutralizing antibodies against SARS-CoV-2 [89] . In another approach, spherical tobacco mosaic virus particles, previously shown to enhance the immunogenic potential of a rabies vaccine [102] , have been applied for COVID-19 vaccine development [90] . Among the expression systems utilized for protein subunit vaccines, baculovirus-based expression of the SARS-CoV S protein has previously induced high titre SARS-CoV-specific neutralizing antibodies in mice [103] and therefore represents a potential alternative approach for vaccine development against COVID-19 [91] . There are numerous preclinical COVID-19 vaccine programs using nonreplicating viral vectors based on adenoviruses, parainfluenza virus, rabies virus and vaccinia virus. For instance, the vaccine based on the adenovirus vector ChAdOx1 expressing the SARS-CoV-2 S protein generated strong humoral and cell-mediated immune responses in mice [92] . Furthermore, a single immunization induced humoral and cellular responses in rhesus macaques and a significantly reduced viral load and absence of pneumonia. In the case of parainfluenza virus-based vaccines, it was shown that a single-dose of intranasal immunization of a parainfluenza virus 5 vector expressing the MERS-CoV S protein induced neutralizing antibody and T-cell responses in mice [93] . Furthermore, a single intranasal administration of 10 4 recombinant parainfluenza virus 5 particles provided protection against challenges with lethal doses of MERS-CoV. The proof of concept demonstrated for MERS in mice, presents the basis for the development of human vaccines for MERS and COVID-19. In the context of rabies virus, proof of concept has been established for recombinant expression of HIV-1, MERS-CoV, Ebola virus and hepatitis C sequences [94] , which makes it a potential vector for vaccine development against COVID-19. Additionally, rabies virus infections target the CNS and the nicotinic acetylcholine receptor in a similar way as has been postulated for SARS-CoV-2, which binds to the nicotine acetylcholine receptor after orthograde or retrograde transport into the CNS. Modified Vaccinia Ankara has been successfully applied for vaccine development against both Ebola virus [104] and Lassa virus [105] and is therefore an attractive candidate for COVID-19 vaccine development. The Modified Vaccinia Ankara-based replication-deficient expression platform has now been mobilized for the preparation of COVID-19 vaccines, currently at the preclinical stage [95] . In another approach, a live-attenuated replication-proficient measles virus was engineered for the expression of CHIKV-like particles, which protected immunized mice from lethal challenges with CHIKV [106] and showed immunogenicity, safety and tolerability in a double-blind, randomized, placebo-controlled Phase II clinical trial [107] . Encouraged by the results from the MV-CHIKV vaccine studies, a preclinical vaccine program was initiated for an MV-based COVID-19 vaccine [96] .

Several preclinical vaccine studies using DNA plasmid-based delivery are in progress. For example, a synthetic DNA-based vaccine targeting the SARS-CoV-2 S protein showed robust expression in vitro and elicited antigenspecific T cells responses and functional antibodies in immunized mice and guinea pigs [97] . The antibodies neutralized the SARS-CoV-2 infection and blocked S protein binding to the ACE2 receptor. Moreover, a series of DNA vaccine candidates expressing different forms of SARS-CoV-2 S showed humoral and cellular immune responses in rhesus macaques [98] . When challenged with SARS-CoV-2, the full-length S vaccine resulted in significant reduction in viral loads and protection. Similar to DNA-based vaccine development, RNA delivery has also been investigated including nanoparticle and liposome-encapsulated RNA as well as self-replicating RNA vectors [108] . In the case of liposome-encapsulated CHIKV mRNA, intravenous administration provided protection against CHIKV challenges in immunized mice [109] , which has paved the way to evaluate mRNA-based approaches for COVID-19 vaccines. In the context of mRNA-based SARS-CoV-2 vaccines, candidates have been selected based on quality criteria and biological activity from preclinical trials [99] . Another approach has been to use self-amplifying RNA for vaccine development against COVID-19 [100] .

In the context of clinical trials, several trials ranging from Phase I to III are in progress and the most advanced ones are described based on published preliminary results from a Phase I study with an Ad-based vector (Table 4 ) [110] . In the case of inactivated COVID-19 vaccine produced in Vero cells, healthy volunteers will be subjected to immunization with different doses and exploration of immunogenicity and vaccine persistence in two Phase I/II trials [111, 112] . It is anticipated that clinical trials will be completed by the end of the year and the vaccine to reach the market early next year [113] . Another purified inactivated SARS-CoV-2 virus vaccine candidate induced SARS-CoV-2-specific neutralizing antibodies in rodents and nonhuman primates and also provided complete protection in macaques [114] supporting the initiation of clinical trials [115] . A total of 422 subjects, age 60 or older, will be future science group www.futuremedicine.com enrolled in a randomized, double-blinded, single-centre, placebo-controlled Phase I/II trial to evaluate the safety and immunogenicity of the vaccine. In a similar study, 774 healthy individuals aged 18-59 years are enrolled [116] . In a Phase I trial on an inactivated SARS-CoV-2 vaccine, 942 healthy volunteers have been enrolled in May 2020 in China [117] . The protein subunit vaccine NVX-CoV2373 is a stable, prefusion protein of the full-length SARS-CoV-2 S applying nanoparticle technology and the saponin-based Matrix™ adjuvant, known for its induction of strong cellular activation of both Th1 and Th2 types, eliciting robust antibody and cytotoxic T-cell responses [118] . The Phase I/II study will be conducted with the SARS-CoV-2 rS nanoparticle vaccine with or without Matrix™ adjuvant in healthy volunteers aged 18-59 years [119, 120] . Preliminary immunogenicity and safety results are expected from the Phase I part of the trial shortly and additional data in the randomized, observer-blinded, placebo-controlled Phase II part later. Several clinical COVID-19 vaccine trials are in progress using Ad vectors. For instance, A Phase I/II study for the ChAdOx1 nCoV-19 vaccine in healthy adults is in progress in the UK to assess the safety, tolerability and reactogenicity of the vaccine [121] . Currently more than 1000 immunizations have been carried out and follow-up is ongoing [122] . Moreover, a Phase III trial for the ChAdOx1 nCoV-19 vaccine, which aims at enrolling up to 10,260 adults and children has started [123] . Moreover, the Ad 5 vector expressing the SARS-CoV-2 S protein was evaluated for safety, tolerability and immunogenicity in a dose-escalation, open-label, nonrandomized, first-inhuman trial in China [124, 125] . Three doses of 5 × 10 10 , 1 × 10 11 and 1.5 × 10 11 viral particles were administered intramuscularly in 108 healthy adults aged 18-60 years. Adverse reactions in the form of injection site pain were mild to moderate and no serious adverse events were recorded by day 28 post vaccination. Neutralizing antibodies were discovered in vaccinees showing a peak humoral response against SARS-CoV-2 at 28 days post vaccination and rapid specific T-cell responses from day 14. More information on the safety and immunogenicity will be received on the Ad-based COVID-19 vaccine from the ongoing Phase II trial [126] . An interesting approach for COVID-19 vaccine development comprise the application of lentivirus transduced dendritic cells (DCs). It has previously been demonstrated that immunization of mice with DCs transduced with lentivirus vectors expressing CD40L and the HIV-1 SL9 epitope induced enhanced antigen-specific T cell proliferation and memory differentiation [133] . In this context, a Phase I trial applying DCs transduced with a lentivirus vector expressing the SARS-CoV-2 S protein (LV-SMENP) has been initiated in China [127] .

In the context of nucleic acid-based vaccines, encouraging results from preclinical studies [97] has permitted the initiation of a Phase I clinical trial on a DNA-based SARS-CoV-2 vaccine in 40 healthy volunteers and plans have been made to initiate a Phase II/III follow-up study [128] . Related to RNA-based vaccines, liposome encapsulated mRNA has previously been demonstrated to be efficient for immunization against CHIKV [97] and has also been subjected to vaccine development for COVID-19 [129] . Liposome nanoparticles containing mRNA encoding a prefusion stabilized SARS-CoV-2 S protein was used as the vaccine candidate in a Phase I, open-label, dose-ranging clinical trial to evaluate the safety, reactogenicity and immunogenicity in 150 healthy volunteers [129, 130] . Preliminary results from eight participants showed that immunization induced neutralizing antibodies to SARS-CoV-2 at levels for recovered COVID-19 patients for the lower dose of 25 μg while the higher dose of 100 μg elicited significantly higher levels of SARS-CoV-2 antibodies [131] . In addition, the first cohort of healthy adults have been enrolled in a Phase II trial [129] . In total, 300 adult participants aged 18-54 years and another 300 volunteers aged 55 years or older will be enrolled. Moreover, a Phase III study protocol for a randomized, placebo-controlled trial with 30,000 participants has been finalized [129] . Finally, a Phase I/II randomized, placebo-controlled observer-blind, dose-finding trial with four SARS-CoV-2 RNA vaccine candidates has been initiated in healthy volunteers [132] . The safety, tolerability, immunogenicity and potential efficacy at three different dose levels in three age groups, ranging from 18-55, 65-85 and 18-85 years, will be evaluated.

Ultimately, to overcome the COVID-19 pandemic the development of broadly available functional vaccines is of outmost importance and the highest priority [20] . The struggle generally seen with development of efficient antiviral drugs and the necessity of vaccinating the majority of the global population cannot be overstated for regaining the confidence in 'life returning to normal'. Publicly, the burning questions have been whether an efficient vaccine can be developed and if so, when will it happen? As presented in this review the efforts to successfully develop drugs and vaccines against COVID-19 can be described as unprecedented and have never before reached the size and spectrum of research and development activities bringing together scientists and clinicians from both academic institutions and the pharmaceutical and biotech industry. Although preliminary results from several clinical trials have been encouraging there is no guarantee that the pandemic can be tamed by drugs or vaccines in the near future. Clearly, the accelerated recovery observed for patients treated with remdesivir, the potential of novel drugs targeting ACE2, monoclonal antibodies and RNAi-based gene silencing, although at a relatively early stage of development, are promising. Moreover, the numerous preclinical and the double-digit clinical trials on vaccines have given hope for achieving prophylactic protection for large populations. Obviously, all possible approaches should be considered and therefore the therapeutic potential of plasma from convalescent COVID-19 patients should not be overlooked as it has previously been demonstrated successful for SARS and MERS [134] . In the context of COVID-19, several studies have been conducted [135] . For instance, in a case study, plasma from six donors the anti-SARS-CoV-2 IgM antibody was weakly reactive showing optical density ratios from 1.22 to 2.01 detected by ELISA for all other donors except for one donor with a slightly higher optical density ratio of 5.63 [136] . All donors except one showed high IgG titres (≥1:320), which made them eligible donors. Treatment of a patient with severe COVID-19 allowed release from mechanical ventilation 11 days after the treatment and was then transferred to a general ward.

Although the competition, especially to obtain an efficacious vaccine, is fierce and the spirit is characterized by 'the winner takes it all', the impressive diversity of approaches should enhance the probability of success. However, most likely there will be a need for several types of drugs and vaccines due to manufacturing characteristics, the function, target population, longevity and range of activity of drugs or vaccines. In any case, the next 6-12 months will be intense and exciting to follow the drug and vaccine development. It cannot be overstated that for not only medical, but also social and economic reasons we need to overcome the current pandemic and to be better prepared for new waves of COVID-19 and other emerging viruses. It is therefore appropriate to join all efforts to together overcome the COVID-19 pandemic illustrated by the rainbow symbol painted by children all over the world with the message 'everything will be alright'.

The author has no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

No writing assistance was utilized in the production of this manuscript. future science group www.futuremedicine.com

• The COVID-19 pandemic has caused unprecedented medical, social and economic damage globally highlighting the need for efficient antiviral drugs and vaccines. Repurposing drugs • A number of studies on repurposed drugs originally developed and/or approved for other viral infections have been subjected to safety and efficacy studies for COVID-19. Novel antiviral drugs • Novel antiviral drugs targeting viral entry and replication of SARS-coronavirus-2 (SARS-CoV-2) have been designed by computational methods and tested in preclinical animal models. 

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Indomethacin and resveratrol as potential treatment adjuncts for SARS-CoV-2/COVID-19

Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved αketoamide inhibitors

Therapeutic strategies in an outbreak scenario to treat the novel coronavirus originating in Wuhan, China

A 193-amino acid fragment of the SARS coronavirus S protein efficiently binds angiotensin-converting enzyme 2

Structure, function and antigenicity of the SARS-CoV-2 spike glycoprotein

Potent binding of novel 2019 coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody

Losartan: a review of its use in stroke risk reduction in patients with hypertension and left ventricular hypertrophy

Comparative efficacy and safety of second-line antiretroviral therapy for treatment of HIV/AIDS: a systematic review and network meta-analysis

Lopinavir-ritonavir in the treatment of COVID-19: a dynamic systematic benefit-risk assessment

Favipiravir (T-705), a broad spectrum inhibitor of viral RNA polymerase

A review on possible modes of chloroquine/hydroxychloroquine: repurposing against SARS-CoV-2 (COVID-19) pandemic

Effect of high vs low doses of chloroquine diphosphate as adjunctive therapy for patients hospitalized with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection: a randomized clinical trial

The QT interval in patients with COVID-19 treated with hydroxychloroquine/azithromycin

Camostat mesylate therapy for COVID-19

SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor

Camostat mesylate in COVID-19 outpatients

Knowledge-based repositioning of the anti-HCV direct antiviral agent sofosbuvir as SARS-CoV-2 treatment

Candidate drugs against SARS-CoV-2 and COVID-19

Antiviral efficacy of flavonoids against enterovirus 71 infection in vitro and in newborn mice

Effective inhibition of MERS-CoV infection by resveratrol

Structure of M(pro) from SARS-CoV-2 and discovery of its inhibitors

A truncated receptor-binding domain of MERS-CoV spike protein potently inhibits MERS-CoV infection and induces strong neutralizing antibody responses: implication for developing therapeutics and vaccines

IgG Fc engineering to modulate antibody effector functions

Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus

Viral infection modulation and neutralization by camelid nanobodies

Selection of nanobodies with broad neutralizing potential against primary HIV-1 strains using soluble subtype C gp140 envelope trimers

A human monoclonal antibody blocking SARS-CoV-2 infection

Angiotensin receptor blockers as tentative SARS-CoV-2 therapeutics

The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complimentary to lin-14

Micro-RNA in disease and gene therapy

Mammalian RNAi for the masses

Silencing SARS-CoV Spike protein expression in cultured cells by RNA interference

Prophylactic and therapeutic effects of small interfering RNA targeting SARS-coronavirus

Proof of concept of siRNA-based gene silencing of SARS-coronavirus (SARS-CoV) replication to encourage design of siRNA sequences for SARS-CoV-2 as in reference 80

RNAi therapeutics: Can siRNA conquer SARS?

Investigation of the functional roles of host cell proteins involved in coronavirus infection using highly specific and scalable RNA interference (RNAi) approach

siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero cells

Design of Potential RNAi (miRNA and siRNA) Molecules for Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Gene Silencing by Computational Method

Short hairpin RNAs targeting M and N genes reduce replication of porcine deltacoronavirus in ST cells

Computational identification of small RNA targets in SARS-CoV-2

The 2019 novel coronavirus resource

WHO. DRAFT landscape of COVID-19 candidate vaccines

The COVID-19 vaccine development landscape

Codagenix and serum institute of india initiate co-development of a scalable, live-attenuated vaccine against the 2019 novel coronavirus, COVID-19

Development of an inactivated vaccine candidate, BBIBP-CorV, with potent protection against SARS-CoV-2

• Successful preclinical validation of production of neutralizing antibodies against SARS-CoV-2 in rodents and primates

CEPI and CSL partner to advance development and manufacture of COVID-19 vaccine candidate

The SARS-CoV-2 pipeline: an overview

Sanofi and GSK to join forces in unprecedented vaccine collaboration to fight COVID-19

ChAdOx1 nCov-19 vaccination prevents SARS-CoV-2 pneumonia in rhesus macaques

Protection against challenges with SARS-CoV-2 in primates preventing pneumonia

Single-dose, intranasal immunization with recombinant parainfluenza virus 5 expressing middle east respiratory syndrome coronavirus (MERS-CoV) spike protein protects mice from fatal MERS-CoV infection

A novel vaccine employing non-replicating rabies virus expressing chimeric SARS-CoV-2 spike protein domains: functional inhibition of viral/nicotinic acetylcholine receptor complexes

Themis collaborates with ABL Europe to manufacture its SARS-CoV-2 vaccine candidate in France

DNA vaccine protection against SARS-CoV-2 in rhesus macaques

Protection of DNA vaccine against challenges with SARS-CoV-2 in primates

About CureVac's activities regarding an mRNA-based vaccine against COVID-19

RNA manufacturing platform

Immunosilencing a highly immunogenic protein trimerization domain

Spherical particles derived from TMV virions enhance the protective properties of the rabies vaccine

A recombinant baculovirus-expressed S glycoprotein vaccine elicits high titers of SARS-associated coronavirus (SARS-CoV) neutralizing antibodies in mice

A single dose of modified vaccinia ankara expressing ebola virus like particles protects nonhuman primates from lethal ebola virus challenge

a single dose of modified vaccinia ankara expressing lassa virus-like particles protects mice from lethal intra-cerebral virus challenge

A recombinant measles vaccine expressing chikungunya virus-like particles is strongly immunogenic and protects mice from lethal challenge with chikungunya virus

Immunogenicity, safety and tolerability of the measles-vectored chikungunya virus vaccine MV-CHIK: a double-blind, randomised, placebo-controlled and active-controlled Phase II trial

Self-replicating RNA viruses for RNA therapeutics

A lipid-encapsulated mRNA encoding a potently neutralizing human monoclonal antibody protects against chikungunya infection

Adenovirus vectors for gene therapy, vaccination and cancer gene therapy

double-blind, placebo parallel-controlled Phase I/II clinical trial for inactivated Novel Coronavirus Pneumonia vaccine (Vero cells

A Phase I/II clinical trial for inactivated novel coronavirus (2019-CoV) vaccine (Vero cells

China steps up inactivated COVID-19 vaccine development

Development of an inactivated vaccine candidate for SARS-CoV-2

Safety and immunogenicity study of inactivated vaccine for prevention of SARS-CoV-2 infection (COVID-19)

Safety and immunogenicity study of inactivated vaccine for prophylaxis of SARS CoV-2 infection (COVID-19). NCT04352608

The updated list of COVID-19 vaccines

Matrix-M adjuvant enhances antibody, cellular and protective immune responses of a Zaire Ebola/Makona virus glycoprotein (GP) nanoparticle vaccine

Evaluation of the safety and immunogenicity of a SARS-CoV-2 rS (COVID-19) nanoparticle vaccine with/without matrix-M adjuvant

A Phase I/II study to determine efficacy, safety and immunogenicity of the candidate coronavirus disease (COVID-19) vaccine ChAdOx1 nCoV-19 in UK healthy adult volunteers

A Phase II/III study to determine the efficacy, safety and immunogenicity of the candidate coronavirus disease (COVID-19) vaccine ChAdOx1

A Phase I clinical trial for recombinant novel coronavirus (2019-COV) vaccine (adenoviral vector) ChiCTR2000030906

Neutralizing antibodies against SARS-CoV-2 detected in vaccinated healthy volunteers after immunization with adenovirus-based vaccine

Safety, tolerability and immunogenicity of a recombinant adenovirus type-5 vectored COVID-19 vaccine: a dose-escalation, open-label, non-randomised, first-in-human trial

double-blinded, placebo-controlled Phase II clinical trial for Recombinant Novel Coronavirus (2019-nCOV) Vaccine (Adenovirus Vector) in healthy adults aged above 18 years

China's Shenzhen Geno-Immune Medical Institute pursues a COVID-19 vaccine

Moderna advances late-stage development of its vaccine (mRNA-1273) against COVID-19

Safety and immunogenicity study of 2019-nCoV Vaccine (mRNA-1273) for prophylaxis of SARS-CoV-2 infection (COVID-19). NCT04283461

Moderna's latest vaccine results are promising -but it's still too early

Significantly higher levels of SARS-CoV-2-specific antibodies found in individuals vaccinated with mRNA-based vaccine compared to COVID-19 patients

Study to describe the safety, tolerability, immunogenicity and efficacy of rna vaccine candidates against COVID-19 in healthy adults

Lentiviral vector-based dendritic cell vaccine suppresses HIV replication in humanized mice

Deployment of convalescent plasma for the prevention and treatment of COVID-19

Convalescent plasma transfusion for the treatment of COVID-19: systemic review

Anti-SARS-CoV-2 virus antibody levels in convalescent plasma of six donors who have recovered from COVID-19