key: cord-0683763-0t258jfk
authors: Lv, Qi; Zhou, Shasha; Qi, Feifei; Zhang, Yaqing; Li, Fengdi; Liu, Mingya; Bao, Linlin
title: Sequentially immune‐induced antibodies could cross‐neutralize SARS‐CoV‐2 variants
date: 2022-02-25
journal: Animal Model Exp Med
DOI: 10.1002/ame2.12216
sha: 5cf815a5de60d35b19af7704f76840a95608d84c
doc_id: 683763
cord_uid: 0t258jfk

BACKGROUND: The Omicron (B.1.1.529) SARS‐COV‐2 variant has raised serious concerns because of its unprecedented rapid rate of spreading and the fact that there are 36 mutations in the spike protein. Since the vaccine‐induced neutralizing antibody targets are the spike protein, this may lead to the possibility of vaccine‐induced humoral immunity escape. METHODS: We measured the neutralizing activity in vitro for Omicron and compared this with wild type (WH‐09) and Delta variants in human and monkey sera from different types of immunity. The monkey sera samples were collected at 1 and 3 months post three‐dose inactivated (PiCoVacc) and recombinant protein (ZF2001) vaccination. Human sera were collected from 1 month post three‐dose inactivated vaccination. RESULTS: In inactivated vaccine sera, at 1/3 months post three‐dose, geometric mean titers (GMTs) of neutralization antibody (NAb) against the Omicron variant were 4.9/5.2‐fold lower than those of the wild type. In recombinant protein vaccine sera, GMTs of NAb against Omicron were 15.7/8.9‐fold lower than those of the wild type. In human sera, at 1 month post three‐dose inactivated vaccination, GMTs of NAb against Omicron were 3.1‐fold lower than those of the wild type. CONCLUSION: This study demonstrated that despite a reduction in neutralization titers, cross‐neutralizing activity against Omicron and Delta variants was still observed after three doses of inactivated and recombinant protein vaccination.

compromised. Compared with other mutants, the Omicron variant contains more than 15 changes in RBD domain of the spike protein, which is the main target of vaccine-induced immunity. Based on the number and location of replacements, as well as on data from other variants with similar spike protein replacements, a significant reduction in serum neutralization activity is expected in vaccinated or previously infected individuals, which may indicate reduced protection against infection. A recent study showed that the secondary breakthrough infection rate of household confirmed Omicron was about 21.6%, which is twice that of the Delta variant. [1] [2] [3] In clinical trials, SARS-COV-2 vaccines have shown absolute health benefits by inducing neutralizing humoral and cellular immunity and reducing the number of COVID-19 infections, hospitalizations, and deaths. However, it has now been shown that neutralizing antibody responses and vaccine potency vary from vaccine dose to vaccine dose, decrease over time after vaccination, and are negatively affected by emerging mutations. 4, 5 The situation is further exacerbated by the observation that neutralizing antibody levels and vaccine protection decreased six months after vaccination, and it has been agreed that protection against emerging variants should be enhanced by increasing doses. However, while vaccines against early variants have been shown to effectively neutralize other variants, it is not clear whether this correlation will be maintained in immune-boosting and highly mutated variants like Omicron.

To answer these questions, we measured the neutralizing activity in vitro for Omicron and compared it with wild type (WH-09) and Delta variants in sera from humans and monkeys with different levels of immunity. The monkey sera samples were collected at 1 and Eagle's medium (DMEM, Invitrogen) supplemented with 10% fetal bovine serum, 100 IU/mL penicillin, and 100 μg/mL streptomycin, and incubated at 37°C, 5% CO 2 . Titers for SARS-CoV-2 were resolved by a 50% tissue-culture infectious doses (TCID 50 ) assay.

The virus droplets were 10-fold diluted and inoculated into simple VeroE6 cells, incubated at 37℃, One hour later, the dilute solution was added to 200 μL DMEM medium with 2% fetal bovine serum, 100 U/mL penicillin and 100 μg/mL streptomycin, and incubated at 37°C, 5% CO 2 . Three days later, we observed cytopathic effects.

The Reed-Muench method was used to calculated tissue culture infective dose (TCID 50 ). 6 

We used cytopathic effect (CPE) tests to detect the presence of neutralizing antibodies. Briefly, human or monkey sera samples were heat inactivated at 56°C. Thirty minutes later, we diluted the sera samples 2-fold serially and then incubated them with 100 TCID 50 SARS-CoV-2 at 37°C, One hour later the samples were added to Vero-E6 cells in a 96-well-plate, and cultured at 37°C.

After 3-4 days, we observed cytopathic effects, and the sera dilution in which 50% of the cells were protected against infection was calculated. 7

Rhesus macaques (4-7 years old) were divided into two groups. One 

We use GraphPad Prism 8.0 software (GraphPad Software, Inc) to analyze all data, using a two-tailed unpaired Student's t test for comparisons among groups. The levels of statistical significance were determined as p < 0.05 (*), p < 0.01 (**).

We measured the neutralizing activity in vitro for Omicron and 

We measured the neutralizing activity in vitro for Omicron compared with wild type (WH-09) and Delta variants in monkey sera samples collected at 1 and 3 months post three-dose recombinant protein vaccination, GMTs of NAb for sera against wild type (WH-09), Delta and Omicron variants were found to be 210.5/69.9, 219.9/52.6, and 13.4/7.9, respectively. GMTs of NAb against the Omicron variant was 15.7/8.9-fold lower than those of the wild type virus (Figure 2 ).

We measured the neutralizing activity in vitro for Omicron compared with wild type (WH-09) in human sera samples collected at 1 month post three-dose inactivated vaccination. GMTs of NAb for sera against wild type (WH-09) and Delta were found to be 34.0 and 10.8, respectively. GMTs of NAb against the Omicron variant was 3.1-fold lower than that of the wild type virus (Figure 3 ).

In the absence of Omicron mutation-specific vaccines, approved vaccines remain the strategy to reduce severe illness and high mortality caused by current circulating strains, including F I G U R E 1 The neutralizing antibody titers for different viruses after three doses of inactivated vaccine. The neutralizing antibody titers against wild type (WH-09), delta, omicron strains in sera of rhesus monkeys at 1 and 3 months after inoculation with three inactivated vaccines. Error bar represents SD from eight independent experiments (t test, *p < 0.05, **p < 0.01, ***p < 0.001) Omicron. 8 This study is a preliminary assessment of the neutralization for Omicron variant using vaccine sera to explore whether the Omicron variant can escape the immunity barrier previously Compared to inactivated vaccine, recombinant protein vaccine had a lower neutralizing activity, which might be related to the extensive epitope coverage in inactivated vaccines, which induces an immune response against the entire virus particle, thereby reducing the vaccine's ability to neutralize newly emerging mutations. 12 The limitations of our study included the relatively small sample sets and limited longitudinal data, so further larger-scale studies need to be carried out to assess the level and durability of protection against Omicron provided by different types of vaccines.

The neutralizing antibody titers for different viruses after three doses of protein vaccine. The neutralizing antibody titers against wild type (WH-09), delta, omicron strains in sera of rhesus monkeys at 1 and 3 months after inoculation with three recombinant protein vaccines. Error bar represents SD from eight independent experiments (t test, *p < 0.05, **p < 0.01, ***p < 0.001) 

The authors declared no conflict of interest. 

The neutralizing antibody titers of human sera for different viruses. The neutralizing antibody titers against wild type (WH-09) and omicron strains in human sera at 1 month after inoculation with three inactivated vaccines. Error bar represents SD from six independent experiments (t test, *p < 0.05, **p < 0.01, ***p < 0.001)

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