key: cord-0681989-fgu1r3b5
authors: Tariq, Mohammad A.; Carding, Simon R.
title: Complete Genome Sequence of a Bacteroides fragilis Bacteriophage, vB_BfrS_NCTC
date: 2021-07-22
journal: Microbiology resource announcements
DOI: 10.1128/mra.00548-21
sha: b8281b723bfd74cad9a73ba64f19b70380b77861
doc_id: 681989
cord_uid: fgu1r3b5

Bacteroides fragilis is an obligate anaerobe and a common gut commensal bacterium that is also an important opportunistic pathogen. Here, we present the complete genome sequence of the circularly permuted B. fragilis bacteriophage vB_BfrS_NCTC. It comprises 47,160 bp, with 69 open reading frames.

The sequencing of vB_BfrS_NCTC yielded 1,012,042 clean reads, with a mean length of 129 bp. The reads had an average Phred quality score of 35, and the GC content was 39.56%. A total of 98.54% of the reads mapped back to the genome, giving a mean coverage depth of 2,793Â. The assembled genome was shown to be circularly permuted at 47,160 bp, with a GC content of 38.83%. A total of 69 ORFs, with no tRNA genes, were identified. The genome comparison was performed using the default settings of the online tool BLASTN with the nonredundant/nucleotide database (16) and showed the greatest similarity at the nucleotide level to the B. fragilis phage Barc2635 (GenBank accession number MN078104), with 92% query coverage and 97% identity.

Data availability. The genome sequence of vB_BfrS_NCTC is available in GenBank under the accession number MW314138. The SRA data can be found under the accession number SRR13174062.

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This work was supported in part by the UK Biotechnology and Biological Sciences Research Council (BBSRC) and BBSRC Institute Strategic Program grant BB/R012490/1 to the Gut Microbes and Health Research Programme and its constituent projects BBS/E/F/ 000PR10353 and BBS/E/F/000PR10356 410 (S.R.C.).We thank Dave Baker and Steven Rudder for sequencing this phage at Quadram Institute Bioscience (Norwich, UK) during the coronavirus disease 2019 (COVID-19) pandemic.