key: cord-0325662-0pocrjvk
authors: Booeshaghi, A. Sina; Pachter, Lior
title: Decrease in ACE2 mRNA expression in aged mouse lung
date: 2020-04-05
journal: bioRxiv
DOI: 10.1101/2020.04.02.021451
sha: 84049adb0bb85cca9770812ae9cc0cafd3e9e8c7
doc_id: 325662
cord_uid: 0pocrjvk

Angiotensin-converting enzyme 2 (ACE2) has been identified as a critical receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This has led to extensive speculation on the role of ACE2 in disease severity, and in particular, whether variation in its expression can explain higher mortality in older individuals. We examine this question in mouse lung and show that 24-month old mice have significantly reduced ACE2 mRNA expression relative to 3-month old mice. The differences appear to be localized to ciliated cells.

The severity of illness resulting from infection with SARS-CoV-2 virus 1 is strongly correlated with age 2 . There has been much speculation about the mechanisms underlying this strong association, with one hypothesis being that age-related changes in ACE2 , a crucial receptor used by SARS-CoV-2 to enter cells 3 , affect disease susceptibility 4 . Nevertheless, there has been little data on the age dependence of ACE2 expression in humans, with the exception of GTEx data, which is limited in the age range to adults 5 .

To better assess the relationship between age and ACE2 expression, we examined single-cell RNA-seq data from eight 3-month old and seven 24-month old mouse lungs 6 in order to identify cell-type specific age-related differences, if any, in ACE2 mRNA expression. Single-cell RNA-seq can determine whether bulk shifts in gene expression are due to changes in cell-type abundances, or changes in gene expression within specific cell types 7 . We find that both cell-type abundance shifts, as well as ACE2 mRNA changes in specific cell types, contribute to a decrease in ACE2 mRNA with age. We corroborate our findings with bulk RNA-seq analysis and ACE2 protein measurement in rat lungs 8 .

To examine mRNA abundance of ACE2 in young and old mice lungs, we began by uniformly processing 9,10 all RNA-seq from GSE12487 6 (see Methods), followed by computation of common coordinates for the 3-month and 24-month old mice single-cell RNA-seq using scVI 11 (Figure 1a , Methods). Consistent with previous findings 12 , we find that ACE2 is not prevalent in the lung, with the exception of Goblet/Club and ciliated cells (Supp. Fig. 1 ). In cells where ACE2 mRNA molecules are identified, the average copy number is 1.19. We, therefore, measured the expression level of ACE2 by the proportion of cells with at least one copy (Methods).

In a comparison of the 3-month old and 24-month old mice, we found significantly fewer cells with ACE2 expression in the 24-month old mice (2.1 fold reduction, p = 0.0375, Figure 1b) . We corroborated this result with an analysis of three bulk RNA-seq samples from 3-month old mice and three bulk RNA-seq samples from 22-month old mice (1.5 fold reduction, p = 0.0163, Supp. Fig. 2 ). This reduction in ACE2 mRNA expression is also seen in an analysis of FACS sorted alveolar macrophage bulk RNA-seq samples (2.7 fold reduction, p = 0.3076, Supp. Fig 3) . These results are consistent with a previously observed dramatic reduction of ACE2 protein between 3-month old and 24-month old rat lungs 8 .

The decrease in ACE2 mRNA expression with age in lungs is likely due to two underlying phenomena: a reduction of ACE2 mRNA in ciliated cells, and a shift in ciliated cell abundance with age (Figure 1c, Supp.  Fig 4) . The latter result is statistically significant and corroborated in an analysis of FACS sorted cells 6 . SARS-CoV-2 uses the serine protease TMPRSS2 for Spike protein priming, so we repeated our analyses for TMPRSS2 . We found no significant changes in mRNA expression between 3-month and 24-month old mice (Supp. Figs. 5-7 ).

Our results show a decrease in ACE2 mRNA expression between young and old mice and contradict recent claims by others 13 . To understand the discrepancies in results, we examined differences in data or methodology that could lead to contradictory results (Supp. Table 1 ). We conclude that there is strong evidence in support of a decrease in ACE2 mRNA between young and old mice, and that other currently available RNA-seq datasets do not contradict this finding. We note that results in rodents may not necessarily translate to humans.

All methods and code to generate the results in this manuscript can be found in the python notebooks at https://github.com/pachterlab/BP_2020 .

Raw FASTQ files can be obtained from GSE124872 .

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