key: cord-0064888-57pmpvak
authors: Barbhuiya, Najmul Haque; Singh, Swatantra P.; Makovitzki, Arik; Narkhede, Pradnya; Oren, Ziv; Adar, Yaakov; Lupu, Edith; Cherry, Lilach; Monash, Arik; Arnusch, Christopher J.
title: Virus Inactivation in Water Using Laser-Induced Graphene Filters
date: 2021-06-09
journal: Materials (Basel)
DOI: 10.3390/ma14123179
sha: eca32045eecdb1ad2aec7c7d5689dfa143898e64
doc_id: 64888
cord_uid: 57pmpvak

Interest in the pathogenesis, detection, and prevention of viral infections has increased broadly in many fields of research over the past year. The development of water treatment technology to combat viral infection by inactivation or disinfection might play a key role in infection prevention in places where drinking water sources are biologically contaminated. Laser-induced graphene (LIG) has antimicrobial and antifouling surface effects mainly because of its electrochemical properties and texture, and LIG-based water filters have been used for the inactivation of bacteria. However, the antiviral activity of LIG-based filters has not yet been explored. Here we show that LIG filters also have antiviral effects by applying electrical potential during filtration of the model prototypic poxvirus Vaccinia lister. This antiviral activity of the LIG filters was compared with its antibacterial activity, which showed that higher voltages were required for the inactivation of viruses compared to that of bacteria. The generation of reactive oxygen species, along with surface electrical effects, played a role in the mechanism of virus inactivation. This new property of LIG highlights its potential for use in water and wastewater treatment for the electrochemical disinfection of various pathogenic microorganisms, including bacteria and viruses.

Worldwide, viral infections alone claim nearly 15 million human lives annually [1] . Vaccination is considered to be an effective preventive measure against viral infection. However, the lack of effective vaccines against different viruses and their variants, combined with the lag time for their development and testing, requires other protection measures for the prevention of viral transmission [2] . Thus, the development of technology that inactivates viruses and bacteria in water and air will prevent viable viruses from entering the host and can help in combating viral infections. Viruses are generally smaller than bacteria, and they consist of viral nucleic acid protected by a proteinaceous layer called the capsid. In some viruses, the capsid can be enclosed by a protective envelope. According to the World Health Organization, many water-transmitted viruses, including adenoviruses, enteroviruses, rotavirus, astrovirus, and hepatitis A and E viruses, pose a moderate to high health risk [3] . Some viruses are also excreted through excreta and have the potential to spread via water [3, 4] . Water can also transmit viruses, for example, via skin and eye contact while swimming or via inhalation while showering, causing ocular and respiratory infections [5] . Conventional wastewater treatment plants can partially remove viruses, but the safe disposal or reuse of effluent highly depends on the final disinfection efficacy [6] [7] [8] [9] . Thus, virus disinfection in wastewater might inhibit the indirect infection pathways [6] and might increase the safety of wastewater reuse. Membrane filtration has the potential to provide a barrier to virus passage; however, electrically conductive surfaces on water filters including graphene can lead to electrochemical surface effects that have been shown to inactivate bacteria. These effects might also cause virus inactivation or removal with no toxic byproduct formation [10] .

Various types of carbon-based materials, including carbon nanotubes, carbon nanospheres, graphene, and their derivatives, have been widely used for water and wastewater treatment [11] [12] [13] . Emerging materials like metal-organic frameworks (MOFs) and LIG can be used to form thin films and have shown promising results for their application in various environmental applications [14] [15] [16] . Among the various materials, graphene is considered to have unique and superior properties. Graphene consists of a single layer of sp 2 -hybridized carbon atoms arranged in a hexagonal lattice [17, 18] . Due to its extraordinary physicochemical properties, graphene and related derivatives have been used in various applications, ranging from electronics to environmental remediation [19] [20] [21] . Recently, researchers demonstrated a novel, cost-effective, and scalable technique for fabricating graphene coatings on various polymeric materials via laser scribing, called laser-induced graphene (LIG) [22, 23] . Certain materials suitable for LIG formation are commonly used in membrane filtration [22] [23] [24] , and the antibacterial and antibiofilm properties of LIG have been explored for water and air filtration [16, [24] [25] [26] [27] . Thus, LIG might enhance water treatment technologies and water filtration, and in the present study, we investigate the ability of porous LIG membranes to inactivate viruses.

Many researchers have explored the various mechanisms by which graphene-based filters can inactivate or kill microorganisms. In the absence of electrical potential, graphene has been demonstrated to rupture cell membranes by inducing mechanical stress on direct contact [28] [29] [30] [31] [32] [33] [34] , to sequester microbial cells [29, [35] [36] [37] [38] [39] , to cause instability in the cell system due to nanoscale dewetting [40] [41] [42] [43] [44] , and to oxidize important cellular components [21, 38, [44] [45] [46] [47] [48] [49] [50] [51] , thereby exacerbating cellular death/inactivation. However, these mechanisms require considerable contact time for microbial inactivation and disinfection. On the other hand, in the presence of electrical potential, conductive filters can instantly remove, kill, or inactivate microorganisms by various mechanisms such as direct and indirect oxidation, Coulombic repulsion, bubble generation, Joule heating, and local pH change [52] [53] [54] [55] [56] [57] [58] [59] . The electrical conductivity of the conductive filters might also contribute to lipid membrane disruption through the electroporation process [21, [60] [61] [62] . Thus, the physicochemical and electrochemical manifold effects of graphene, including LIG filters, can potentially also be useful for the inactivation of viruses. Furthermore, the integration of renewable energy like solar energy with membrane processes can further make the use of LIG filters and membranes more sustainable [63] .

In this study, we demonstrate the antiviral ability of LIG filters against the Vaccinia lister virus, a prototypic poxvirus, at different applied potentials. LIG filters were fabricated using polyethersulfone ultrafiltration membranes and used to test the inactivation of the virus by conducting plaque assays, and the destructive effects on the virion particles were observed via transmission electron microscopy (TEM) analysis. We discuss the possible mechanisms of LIG virion inactivation in comparison to LIG's effects on bacteria. The results show that the LIG-based conductive filters are a promising candidate in providing high-efficiency viral/bacterial disinfection with low energy consumption, which can be explored in different water treatment and membrane filtration processes.

The LIG membrane filters were fabricated via a method similar to that described in our previous work [24] . Briefly, polyethersulfone (PES) (UP150, Microdyn-Nadir, Wiesbaden, Germany) membranes were lased with a focused laser spot using a VLS 3.50 (Universal Laser Systems, (Distributor: Caliber, Tel Aviv, Israel)) laser platform, which was equipped with a 10.6 µm CO 2 pulse laser (50 W, 2.0 inch Lens Kit) (Universal Laser Systems, (Distributor: Caliber, Tel Aviv, Israel)). The LIG was obtained with a laser setting of 70 PPI image density, 25% scan rate, and 0.1% laser duty cycle, made in the presence of air under ambient conditions. The circular LIG filters were~200 µm thick with a radius of 23 mm. The untreated membranes and the LIG filters were characterized by Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and contact angle measurements, both prior to and after laser modification, as previously described [24] .

Carbon wires were attached to circular, LIG-coated PES filters using a carbon-based glue. Two LIG electrode filters were stacked one on top of the other and placed in a deadend filtration unit ( Figure S1 ). Each electrode was connected to a direct current (DC) voltage supply, and unless otherwise stated, the connections were made such that the anode was situated atop the cathode. A secondary connection was made to a multimeter such that the current through the electrodes could be simultaneously monitored. Bacterial testing was performed as previously described using a bacterial culture mix [24] For viral tests, Vaccinia lister in 30 mL phosphate buffer saline (PBS) was passed through the conductive LIG filters using a vacuum pump at a constant flow rate of~1000 L m −2 h −1 in a dead-end filtration mode. Then, the filter was washed with an additional 30 mL PBS. The power was adjusted to 2.5, 5.0, 10.0, and 20.0 V for these filtration experiments. Control experiments in the absence of electrical potential were also performed. The feed and permeate were collected and serially diluted, and the microbes were enumerated using plaque assay for viruses and plate counting methods for bacteria [24] .

In a six-well plate (Costar Corning Incorporated, NY, USA), 7 × 10 5 Vero cells (ATCC CCL-81) in suspension at a volume of 3 mL were plated for each well with DMEM medium supplemented with 10% heat-inactivated FCS (Biological Industries LTD, BET-Haemek, Israel). The cell culture was grown for one day at 37 • C, in 5% CO 2 , to a monolayer. The Vaccinia virus standard curve was diluted to an estimated concentration of 150-600 plaque-forming unit (PFU) mL −1 . Then, 0.3 mL of the virus sample/standard was added to 0.2 mL of medium for each well. The plates were incubated with agitation for 60 min at 37 • C, in 5% CO 2 , with a relative humidity of > 85%. The medium was removed, and 1 mL of 0.1% crystal-violet solution was added and incubated at room temperature for 5 min. The solution was removed, and the cells were washed with water. The plaques were counted manually.

The drop-on-the-grid method (DEG) was used as follows. A drop (10 µL) of sample suspension containing 10 5 Vaccinia viruses was fixated by incubation with 2.5% paraformaldehyde (Electron Microscopy Sciences, PA, USA) for 30 min at rt followed by another 30 min of incubation at 37 • C. Then, the sample was placed directly onto a glow-discharged EM sample support, 300 MESH copper grid, covered with carbon film (Electron Microscopy Sciences, PA, USA). After adsorption for 10 min at room temperature, the grid was washed three times in double-distilled water and negatively stained with 1% phosphotungstic acid, pH 4.5. The grids were examined using a TECNAI T12 FEI (Ther-moFisher, OR, USA) transmission electron microscope operated at 200 kV. Micrographs were recorded using an Erlangsheng 782 ES 500W camera (Gatan, CA, USA) at a resolution of 2048 × 2048 pixels.

The electrogeneration of H 2 O 2 was tested with aqueous solutions of NaCl (0.9% w/w) and Na 2 SO 4 (0.9% w/w). Each solution was passed through the stacked LIG filters under the application of various voltages (2.5 V, 5 V, 10 V, and 20 V) with~1000 L m −2 h −1 flow rate. The concentrations of H 2 O 2 and free chlorine in the permeate were quantified using the DMP and DPD methods, respectively [64] .

The LIG filters using PES membranes as substrates were fabricated according to the method used in our previous study [24] . The filters were characterized using SEM, XPS, and Raman spectroscopy. The SEM images showed a highly porous structure for LIG filters (Figure 1a,b) and a characteristic porous foamlike structure in the cross section (Figure 1a , inset). Contact angle measurements were made for unused (39.7 ± 1.1 • ) and used LIG filters (30.5 ± 2.4 • for the anodic and 28.4 ± 0.9 • for the cathodic filter). The used cathodic and anodic LIG filters were slightly more hydrophilic than the unused filters and suggested that oxidation of the LIG might occur during filtration ( Figure S2 ). XPS revealed the chemical composition information of the material surface (Figure 1c) . It showed C 1s, O 1s, and S 2p peaks at~285.5,~534, and~167 eV, respectively, which was also similar to the results of previous studies [24, 65, 66] . This confirmed the presence of C-S bonding in the LIG skeleton, which was absent in the PES membrane substrate. The Raman spectra showed the characteristic D, G, and 2D peaks for graphene at~1350 cm −1 , 1580 cm −1 , and 2700 cm −1 , respectively (Figure 1d) . A high degree of graphene formation and graphitization was supported by the D/G and 2D/G intensity ratios. 

The LIG filters in an anode-on-cathode stacked configuration were used with an externally applied DC voltage to test their antimicrobial or antiviral activity. The LIG filters were able to inactivate or kill bacteria and virions under the application of different applied voltages (Figure 2a ). At this voltage, 40-60% virus removal was seen, while the same conditions resulted in 100% bacterial removal ( Figure S3 ). However, higher electrical potential (10 V) caused significant damage to viral particles (Figure 3e,f) , and~92% removal was observed. Further testing at 20 V resulted in complete inactivation (>4 log virus removal), and destruction of the virion was seen (Figure 3g,h) . Virion damage might be because of similar mechanisms described for bacterial inactivation, including electrical and chemical effects [26] . However, virions are reported to be more resistant than bacteria towards chemical disinfectants such as chlorine and hydrogen peroxide [67] [68] [69] , and this might explain the differences in the activity. H 2 O 2 and chlorine generation were found to be voltage dependent [24, 26, 70] , which would have increased with increasing voltage, resulting in more indirect oxidation of microbial cellular materials. Also, a higher electric field is required for the electroporation effect in smaller organisms [71] , and this provides a plausible explanation for the relatively higher electrical potential required for viral destruction compared to bacterial destruction (Figure 2c) . The transmembrane voltage induced in the cell membrane of the organism under an applied electric field is proportional to the size of the organism. Thus, a greater field is required for electroporation of smaller virus particles than for bacterial cells [62, [71] [72] [73] [74] . Moreover, since the number concentrations of bacteria and viruses, along with the flow rates, were approximately the same for both bacterial and virion inactivation, the lower inactivation of virions was also probably due to the smaller size of virions (Figure 2b) . Here, less contact with the LIG would result in less direct electrical effects and lower charge transfer, and the indirect chemical effects might play a greater role in the mechanism, displayed by the increased voltage requirement for disinfection [71] . Additionally, an increase in electrical potential can increase the electrophoretic movement of the virions towards the anode, resulting in direct oxidation and increased virus removal; a similar effect was seen for bacterial cells. A detailed explanation of these mechanisms of microbial inactivation by LIG electrodes can be found elsewhere [26] . Briefly, the antimicrobial activity of the LIG filters increases when used as electrode pairs under electrical potentials. The microbial killing possibly involves physical destruction due to contact with the LIG, chemical oxidation, or the electrical effects of LIG electrodes. The movement of microbes towards LIG surfaces, combined with surface toxicity and the generation of localized active chemical species, is the most plausible antimicrobial action mechanism. Also, the concentration of active chemical species is higher near the electrodes than in the bulk solution. Thus, the electrostatic attraction of microbes towards the anode further exposes them to a higher concentration of active chemical species. The LIG electrodes may also cause physical damage to the microbe via irreversible electroporation or direct electron transfer [60, 71, 75, 76] . Taken together, the various antimicrobial effects of conductive LIG filters are also useful for inactivating viruses, but at higher potentials.

The voltage-dependent microbicidal efficiency of LIG filters is consistent with the explanation that an increase in the applied potential over the electrodes leads to an associated increase in the electric field and current density. This causes microbial death via electroporation and mechanical destruction, and redox processes can occur that generate toxic chemical species, which can oxidize structural features of the microbes and cause oxidative-stress-mediated death [26] . Components in the lipid membrane and negatively charged lipids make bacteria anionic. Similarly, in virions, the outer capsid surfaces are generally also negatively charged at near-neutral pH values, and increasing the pH results in increased negative surface charge densities [77, 78] . As a consequence, microbes are predicted to possess a Coulombic attraction to the positively charged anode while being repelled by the negatively charged cathode. This reasoning motivated the placement of the anode above the cathode in the constructed filtration system; the deformation and elongation of cells can occur as they are pulled against the irregular anode surface, thereby affecting the membrane rupture and death of the bacteria [26] . This electrostatically induced mechanical effect is expected to increase as the externally applied voltage is increased [26] . Similarly, the virion particles are destroyed at a higher voltage and can be seen in Figure 2e -h. To confirm that the anode-on-top configuration of LIG filters is most effective, the antimicrobial activity was evaluated as previously, except with the cathodic filter situated above the LIG anode. However, the effect was minor, as we observed that the cathode-on-top filter configuration was only slightly less effective at microbial killing (Table S1 ).

The H 2 O 2 and free chlorine concentrations were measured in the permeate of the stacked filters under 2.5 V, 5 V, 10 V, and 20 V. An increase in H 2 O 2 concentration was observed with an increase in the applied voltage (Figure 3i) . A maximum of~5 mg L −1 of H 2 O 2 was obtained at 20 V with 0.9% NaCl. However,~7 mg L −1 of H 2 O 2 was detected at 20 V with Na 2 SO 4 (0.9%). This difference may be due to the generation of free chlorine at the anode with NaCl. The free chlorine can react with the H 2 O 2 , thus reducing its concentration [26, 64] . However, it should be noted that no free chlorine was detected in the permeate for either solution. In other studies, solutions containing viruses and 5-7 mg L −1 H 2 O 2 concentrations required hours for the observation of virus inactivation (< 0.1-1 log reduction of viruses) [79, 80] . To achieve a 4 log or greater level of virus inactivation, higher doses of H 2 O 2 or longer contact time was required [81] [82] [83] [84] . One study reported the use of 7.5% H 2 O 2 for 20 min for~5 log level inactivation of vaccinia virus [85] . In our present study, we observed~4 log removal with a lower concentration of H 2 O 2 (~5 mg L −1 ) and with a shorter filtration time (~4 min). Thus, the electrochemical effect near the surface of the LIG filters might play an important role in the bacterial and viral inactivation.

The high surface area and intrinsic charge-carrier mobility of LIG filters allow high current densities from relatively low voltages, resulting in the rapid and energy-efficient killing of virions via electrical contact and the formation of redox-active chemical species such as H 2 O 2 at the lower, cathodic filter and Cl 2 at the upper, anodic filter, as seen in the following equations:

2 Cl -(aq) → 2 Cl 2 (g) + 2 e -(1)

The current-voltage relationship for the LIG filters used during experimentation was elucidated using 0.9% NaCl solution (Table S2) . As predicted, the extensive surface area of the LIG filters leads to the generation of large currents and can enhance electrochemical processes, even at low voltages [26] .

Sulfur-doped LIG filters were prepared using PES membranes as substrates. The filters were characterized and were found to be highly porous and electrically conductive. The antiviral activity of the LIG filters was investigated under the application of electrical potential. A Vaccinia virus suspension was filtered through the stacked LIG filters under varied applied potential. The application of low potential was sufficient for 6 log removal of the bacteria; however, a higher potential application was required for 4 log removal of the virus. The virion damage may be due to electrochemical effects, including electroporation, reactive chlorine, and oxygen species generation. The higher electrical potential requirement is due to the smaller size of the virus and its greater resistance towards reactive chemical species like H 2 O 2 . LIG-integrated membranes and filters have the potential to build the next generation of cost-effective systems for simultaneous water filtration and disinfection of pathogenic microbes, including bacteria and viruses.

Supplementary Materials: The following are available online at https://www.mdpi.com/article/10 .3390/ma14123179/s1, Figure S1 : Experimental setup for filtration experiments, Figure S2 : Contactangle of LIG-PES membrane surfaces, Figure S3 : Comparative percentage removal of bacteria and viruses under the application of different electrical potential, Table S1 : Bacterial inactivation at a fixed vacuum pressure and varying LIG electrode configurations, Table S2 : Current-voltage relationship of LIG-PES electrodes. 

The challenge of emerging and re-emerging infectious diseases

Molecular constraints to interspecies transmission of viral pathogens

World Health Organization. Guidelines for Drinking-Water Quality

Review of cytomegalovirus shedding in bodily fluids and relevance to congenital cytomegalovirus infection

Waterborne Viruses: A Barrier to Safe Drinking Water

Rethinking wastewater risks and monitoring in light of the COVID-19 pandemic

Editorial Perspectives: 2019 novel coronavirus (SARS-CoV-2): What is its fate in urban water cycle and how can the water research community respond?

Emerging investigators series: The source and fate of pandemic viruses in the urban water cycle

Assessment of human virus removal during municipal wastewater treatment in Edmonton

Occurrence of a New Generation of Disinfection Byproducts

Carbon based materials: A review of adsorbents for inorganic and organic compounds

Laser-induced graphene and carbon nanotubes as conductive carbon-based materials in environmental technology

Synthesis, fabrication, and mechanism of action of electrically conductive membranes: A review

Adsorptive removal of antibiotic sulfonamide by UiO-66 and ZIF-67 for wastewater treatment

Electrodeposited Metal Organic Framework toward Excellent Hydrogen Sensing in an Ionic Liquid

A Journey of Laser-Induced Graphene in Water Treatment

The rise of graphene

Honeycomb Carbon: A Review of Graphene

Three-dimensional strutted graphene grown by substrate-free sugar blowing for high-power-density supercapacitors

Prospects and Challenges of Graphene in Biomedical Applications

Environmental applications of graphene-based nanomaterials

Laser-induced porous graphene films from commercial polymers

Laser-Induced Graphene by Multiple Lasing: Toward Electronics on Cloth, Paper, and Food

Sulfur-Doped Laser-Induced Porous Graphene Derived from Polysulfone-Class Polymers and Membranes

Laser-Induced Graphene Biofilm Inhibition: Texture Does Matter

Laser-Induced Graphene Layers and Electrodes Prevents Microbial Fouling and Exerts Antimicrobial Action

Self-Sterilizing Laser-Induced Graphene Bacterial Air Filter

Toxicity of a polymer-graphene oxide composite against bacterial planktonic cells, biofilms, and mammalian cells

Graphene Can Wreak Havoc with Cell Membranes

Cellular entry of graphene nanosheets: The role of thickness, oxidation and surface adsorption

Graphene microsheets enter cells through spontaneous membrane penetration at edge asperities and corner sites

Graphene-Based Antibacterial Paper

Graphene Induces Formation of Pores That Kill Spherical and Rod-Shaped Bacteria

Destructive extraction of phospholipids from Escherichia coli membranes by graphene nanosheets

Graphene oxide exhibits broad-spectrum antimicrobial activity against bacterial phytopathogens and fungal conidia by intertwining and membrane perturbation

Lateral Dimension-Dependent Antibacterial Activity of Graphene Oxide Sheets

Wrapping Bacteria by Graphene Nanosheets for Isolation from Environment, Reactivation by Sonication, and Inactivation by Near-Infrared Irradiation

Antimicrobial Properties of Graphene Oxide Nanosheets: Why Size Matters

Antibacterial Activity of Graphite, Graphite Oxide, Graphene Oxide, and Reduced Graphene Oxide: Membrane and Oxidative Stress

Dewetting and Hydrophobic Interaction in Physical and Biological Systems

Hydrophobic Collapse in Multidomain Protein Folding

Observation of a dewetting transition in the collapse of the melittin tetramer

Potential Toxicity of Graphene to Cell Functions via Disrupting Protein-Protein Interactions

Cytotoxicity Effects of Graphene and Single-Wall Carbon Nanotubes in Neural Phaeochromocytoma-Derived PC12 Cells

Oxidative stress-mediated antibacterial activity of graphene oxide and reduced graphene oxide in Pseudomonas aeruginosa

Surface Modification of Membrane Filters Using Graphene and Graphene Oxide-Based Nanomaterials for Bacterial Inactivation and Removal

Interaction of Graphene Oxide with Bacterial Cell Membranes: Insights from Force Spectroscopy

A new function of graphene oxide emerges: Inactivating phytopathogenic bacterium Xanthomonas oryzae pv

Oxidative stress and antibacterial properties of a graphene oxide-cystamine nanohybrid

The triggering of apoptosis in macrophages by pristine graphene through the MAPK and TGF-beta signaling pathways

Electrically conductive polymeric membranes for fouling prevention and detection: A review

Prevention of Pseudomonas aeruginosa adhesion by electric currents

Electrochemical Multiwalled Carbon Nanotube Filter for Viral and Bacterial Removal and Inactivation

Critical review of electrochemical advanced oxidation processes for water treatment applications

Electrochemical biofilm control: Mechanism of action

Bacterial Inhibition by Electrical Stimulation

Electrochemical mineral scale prevention and removal on electrically conducting carbon nanotube-polyamide reverse osmosis membranes

Enhanced Flux and Electrochemical Cleaning of Silicate Scaling on Carbon Nanotube-Coated Membrane Distillation Membranes Treating Geothermal Brines

Electroporation of Cell Membranes

High-Performance Capacitive Deionization Disinfection of Water with Graphene Oxide-graft-Quaternized Chitosan Nanohybrid Electrode Coating

Conducting Nanosponge Electroporation for Affordable and High-Efficiency Disinfection of Bacteria and Viruses in Water

A solar membrane-based wastewater treatment system for high-quality water production

Comparison among the Methods for Hydrogen Peroxide Measurements To Evaluate Advanced Oxidation Processes: Application of a Spectrophotometric Method Using Copper(II) Ion and 2,9-Dimethyl-1,10-phenanthroline

Laser-Induced Graphene-PVA Composites as Robust Electrically Conductive Water Treatment Membranes

Graphene oxide on laser-induced graphene filters for antifouling, electrically conductive ultrafiltration membranes

Comparative electrochemical inactivation of bacteria and bacteriophage

The Inactivation of Bacteria and Viruses by Hydrogen Peroxide

Chlorination of Indicator Bacteria and Viruses in Primary Sewage Effluent

Situ Electrochemical Generation of Reactive Chlorine Species for Efficient Ultrafiltration Membrane Self-Cleaning

Electroporation-based applications in biotechnology

The Influence of Size and Shape of Microorganism on Pulsed Electric Field Inactivation

Locally Enhanced Electric Field Treatment (LEEFT) Promotes the Performance of Ozonation for Bacteria Inactivation by Disrupting the Cell Membrane

Emerging investigator series: Locally enhanced electric field treatment (LEEFT) with nanowire-modified electrodes for water disinfection in pipes

Yeast cell inactivation related to local heating induced by low-intensity electric fields with long-duration pulses

Inactivation of Escherichia coli in the electrochemical disinfection process using a Pt anode

Surface Charge Mapping on Virions and Virus-Like Particles of Helical Plant Viruses

Viruses at Solid-Water Interfaces: A Systematic Assessment of Interactions Driving Adsorption

Inactivation of enteric microorganisms with chemical disinfectants, UV irradiation and combined chemical/UV treatments

Inactivation of E. coli, B. subtilis spores, and MS2, T4, and T7 phage using UV/H2O2 advanced oxidation

Sanitizing agents for virus inactivation and disinfection

Development of a new hydrogen peroxide-based vaccine platform

Hydrogen peroxide inactivation of influenza virus preserves antigenic structure and immunogenicity

Inactivation of rabies virus by hydrogen peroxide

Disinfection efficacy against parvoviruses compared with reference viruses