key: cord-0064116-uhw956z4 authors: Musa, Arafa; Elmaidomy, Abeer H; Sayed, Ahmed M; Alzarea, Sami I; Al-Sanea, Mohammad M; Mostafa, Ehab M; Hendawy, Omina Magdy; Abdelgawad, Mohamed A; Youssif, Khayrya A; Refaat, Hesham; Alaaeldin, Eman; Abdelmohsen, Usama Ramadan title: Cytotoxic Potential, Metabolic Profiling, and Liposomes of Coscinoderma sp. Crude Extract Supported by in silico Analysis date: 2021-06-04 journal: Int J Nanomedicine DOI: 10.2147/ijn.s310720 sha: 602c9e3750b661a1419c850d123ffb04bfe23c6c doc_id: 64116 cord_uid: uhw956z4 INTRODUCTION: Sponge-Coscinoderma sp. (Family: Spongiidae) is a coastal sponge that possesses a broad variety of natural-products. However, the exact chemical constituents and cytotoxic activity of the extract are still undefinable. METHODOLOGY: In the present study, the metabolomic profiling of Coscinoderma sp. dereplicated 20 compounds, utilizing liquid chromatography coupled with high-resolution mass spectrometry (LC-HRESIMS). Coscinoderma-derived crude extract, before and after encapsulation within nanosized liposomes, was in vitro screened against hepatic, breast, and colorectal carcinoma human cell lines (HepG2, MCF-7, and Caco-2, respectively). RESULTS: The identified metabolites were fit to diverse chemical classes, covering diterpenes, an indole alkaloid, sesterterpenoid, sterol, and methylherbipoline salt. Comprehensive in silico experiments predicted several compounds in the sponge-derived extract (eg, compounds 1–15) to have an anticancer potential via targeting multiple targets. The crude extract showed moderate antiproliferative activities towards studied cell lines with IC(50) values range from 10.7 to 12.4 µg/mL. The formulated extract-containing liposomes (size 141±12.3nm, PDI 0.222, zeta potential 20.8 ± 2.3), significantly enhanced the in vitro anticancer activity of the entrapped extract (IC(50) values ranged from 1.7 to 4.1 µg/mL). DISCUSSION: Encapsulation of both the hydrophilic and the lipophilic components of the extract within the lipid-based nanovesicles enhanced the cellular uptake and accessibility of the entrapped cargo. This study introduces liposomal nano-vesicles as a promising approach to improve the therapeutic potential of sponge-derived extracts. The taxonomic biodiversity of coastal living forms had passed up to 30 x10 6 species involving greater than 70% of the earth's surface. However, the total of biologically effective compounds from this enormous origin was restricted to a few thousand. 1 Therefore, it was obvious to predict that marine organisms expressed an exceedingly valuable source of novel bioactive materials that can drive the outcome of different drugs. 2 Natural products from marine origin as sponges and echinoderms had been explored broadly for their biological activities. [3] [4] [5] The spongeCoscinoderma sp. (Family: Spongiidae), fitted to an arrangement of coastal sponges that had a broad variety of natural products, covering diterpenes, sesquiterpene hydroquinones, long-chain aliphatic and acetylenic compounds, furanic and scalarane sesterterpenes, bromotyrosine alkaloids, hepta-and octaprenylhydroquinones, suvanine analogs, and farnesyl quinols, which reported to have antitumor, antimalarial, antibacterial, antifungal, and protein tyrosine phosphatase-1B (PTP1B) inhibitoryactivities. 6 Despite that Cosinoderma has shown a wide range of biological activities, its chemical constituents are still elusive. Consequently, we selected this sponge in the present to shed some light on its chemical makeup and to investigate its potential as anticancer agent depending on a comprehensive in silico predictive study and a subsequent in vitro validation. Liposomes are lipid-based bilayer nanovesicles that are capable of entrapment of both hydrophilic and lipophilic drugs either inside its core or within the phospholipid bilayer, respectively. 7 Many studies have reported the impact of formulating natural products within liposomal nano-vesicles in the entrapment of both hydrophilic and lipophilic constituents, 8 improvement of stability of entrapped cargo, 9 improvement of cellular uptake of that natural components 10 and targeted delivery to the definite site of action. [11] [12] [13] Liposomes have the advantages of being biocompatible, nonimmunogenic, and flexible dosage form that achieves the controlled delivery of the entrapped active constituents. 14, 15 Several techniques have been adopted for the formation of liposomes including thin-film hydration method, 16 spraying technique 8 and ethanol injection method. 17 The ethanol injection method is a favorable technique because it is simple and enables the nonmixing of the organic phase with the aqueous one producing homogenous nanosized vesicles. 18 Despite the wide range of therapeutic benefits of Coscinoderma sp., its low bioavailability, and leakage of suitable formulation retard the clinical application of such promising marine product. Formulating a convenient dosage form that can guarantee the entire inclusion, enhanced stability, and the cellular delivery of its physicochemically diverse components was a necessity. Consequently, the main purposes of the present investigation are to investigate the chemical profile of the Cosinoderma extract, predict the most probable bioactivity of this extract depending on a comprehensive in silico study of its main components, and test this predicted bioactivity in vitro, with study if the biological activity was enhanced upon entrapment of the extract into nanoformulation. Chemicals and reagents used in this study were described in detail on Supplementary Material and Methods -Chemicals and reagents. Freeze-dry sponge material (8g) was extracted with methanol methylene chloride (1:1). The crude extract, developed at 1mg/mL for mass spectrometry analysis. The recovered ethanolic extract was exposed to metabolic analysis using LC-HRESIMS. [19] [20] [21] The details for the LC-HRESIMS method are described on Supplementary Material and Methods -Metabolomic Analysis Procedure. Liposomes were developed by the simple ethanol injection method. 18 The details for the utilized method are described in Supplementary Prepared liposomes of Coscinoderma sp. were imaged using (JEM-1400, Jeol, Tokyo, Japan) equipped at 80 kV. The liposomal suspension was imaged on a carbon-coated copper grid which was left for 10 minutes at 25°C before examination. The effect of temperature on the weight of empty liposomes and Coscinoderma extract either free or encapsulated within the prepared liposomes was studied using Thermogravimetric Analysis (TGA). Samples were dried and 20 dried samples were heated from 30°C to 450°C in a platinum pan (heat flow rate of 20 °C/min and nitrogen flow rate 20mL/min). To gain more insight into the probable interaction between lipoid S75, cholesterol, and Coscinoderma extract, Fourier-transform infrared (FT-IR) measurements were carried out for the Coscinoderma extract, blank liposomes, and Coscinoderma liposomes over the wavenumber range 4000 to 400 cm (Nicolet IS 10 FTIR spectrometer, US) after the dispersion of samples in KBr discs. This study was developed under the guidelines of the United Kingdom Coordinating Committee on Cancer Research (UKCCCR) that addressed the Use of cell lines in cancer research. The cancer cell lines HepG2, MCF7, and Caco-2 culture condition described in Supplementary Material and Methods -Cell Culture Conditions. The antiproliferative activity of Coscinoderma sp.containing liposomes and their corresponding empty liposomes were described in detail in Supplementary Material and Methods -Antiproliferative assay. Molecular docking was carried out utilizing Autodock Vina software. 23 The details were described in Supplementary Material and Methods -Molecular Docking Experiments. The details were described Supplementary Material and Methods -Statistical analysis. fit an indole alkaloid, and antiplasmodial sterol derivative compounds coscinamide A 9, 5α,8α-epidioxycholesta-6-en-3β-ol 10, 5α,8αepidioxy-24-methylcholesta-6,9(11) 24(28)-trien-3β-ol 11, 5α,8α-epidioxycholesta-6,24(28)-dien-3β-o1 12, and (24S)-5α,8α-epidioxy-24-methylcholesta-6-en3β-ol 13, that was previously isolated from Coscinoderma mathewsi, and other Coscinoderma sp., respectively. 25 Morphology of the prepared formulations reveals that vesicular liposomes are successfully prepared ( Figure 4A and B). The vesicles are small and homogenously distributed (size= 131±12.3, PDI=0.222). The vesicles have a zeta potential of 20.8 ± 2.3. Thermogravimetric analysis was carried out to evaluate the potential of encapsulating the extract within the formulated liposomes on the enhancement of the physical and chemical stability of the entrapped Coscinoderma extract as a function of temperature. TGA curves for empty liposomes, Coscinoderma and Coscinoderma liposomes are shown in Figure 5 . Upon heating from 30°C to 450°C, about 72.5% and 16.3% weight loss was observed at a temperature of 169° C for Coscinoderma extract and Coscinoderma liposomes, respectively. Results show the enhanced thermal stability of the entrapped cargo due to liposomal encapsulation. To estimate the possible interactions between the components of the extract and those of the membrane bilayer of liposomes, FTIR spectra of Coscinoderma extract, empty liposomes, and Coscinoderma liposomes were studied ( Figure 6 ). The FTIR spectrum of Coscinoderma extract contains principle bands at 3409, 1622, 1210, and 1507, and that of empty liposomes contains bands at 2907, 1736, 1459, 1234, and 1060. The FTIR spectrum of Coscinoderma liposomes contains similar bands to those contained in both free extract and empty liposome spectra, indicating that the encapsulation of Coscinoderma extract within the prepared liposomes did not form new linkages. Neural networks-based biological activity predictions that depend on artificial intelligence and machine learning processing along with other computer-aided drug design approaches have become widely accepted as an integral step during the drug discovery process. 30, 31 Such in silico-based procedures could-be employed in drug discovery from natural sources, where they can register a set of possibly active hits among a complex mixture of other metabolites present in a given-natural crude extract. 32 To putatively assign the most probable metabolites that might be associated with the anticancer activity of Coscinoderma sp., we submitted the most abundant metabolites ( Figure 3 ) to a neural network-based prediction software PASS. This software search algorithm depends on the structural analogy of a great number of inhibitors recorded for a broad area of biological targets. 23 As shown in Figure 7A , among the detected metabolites in Coscinoderma sp., compounds 1-15 that represented about 76.6% of the detected compounds, were predicted to exhibit antiproliferative activity (Pa > 0.5). Moreover, human phosphatase was suggested to be the probable target for them except for metabolites 3 and 9 that were predicted to target peptidyl prolyl cis-trans-isomerase NIMA interacting-1 (PIN-1). Accordingly, we searched for human phosphatases that are strongly linked to tumorigenesis. We found the non-receptorprotein-tyrosine-phosphatase (SHP2) along with proteintyrosine-phosphatases (PRL-1, -2, and -3) are currently well established as oncogenic phosphatases. 33 These proteins are known to regulate cell survival and proliferation, through activation of the RAS-ERK (extracellular signal-regulated kinase) signaling pathway. 33 On the other hand, Pin-1 is a key effector in Ras signaling and is frequently overexpressed in many types of cancers with poor prognosis. 34 Consequently, we further assessed the PASS predictions by molecular docking experiments against the oncogenic phosphatases (ie, SHP2, and PRL-1 to -3) together with Pin-1. Among the metabolites that were predicted to mediate an anticancer activity by the inhibition of oncogenic phosphatases ( Figures 7B and 8) , only compounds 4-6 achieved good binding affinities ( Figure 5B ; < −5 kcal/mol) toward SHP2, that was also higher than that of the co-crystallized inhibitor (−8.5 kcal/mol for compounds 4-6, and −7.1 kcal/mol for the co-crystal inhibitor). Additionally, the mode of interaction of these metabolites (ie, 4-6) was comparable with this of the reported co-crystallized inhibitor. 35 The most important interactions inside the SHP2's binding site were H-bonding, particularly with ARG-11, PHE-113, and GLU-250, amino acids that were also involved in the interaction with the co-crystallized inhibitor ( Figure 8 ). Regarding Pin-1, both metabolites 3 and 9 were predicted to target this oncoprotein, and they were also achieved good binding affinities toward Pin-1 with a mode of interactions convergent to that of the co-crystallized inhibitor ( Figures 7B and 9 ). 36 Both compounds 3 and 9 interacted through H-bonding with LYS-63, ARG-69, ASP-112, and SER-154. Moreover, they exhibited two hydrophobic interactions with LEU-122 and PHE-134 ( Figure 9 ). These bis-indole derivatives have been previously identified as anticancer agents. 37 According to the results of the in silico analysis, Coscinoderma sp.'s crude extract has a great anticancer potential. Consequently, it was in vitro screened for its potential as antiproliferative against hepatic, breast, and colorectal carcinoma cell lines (HepG2, MCF-7, and Caco-2, respectively). Results revealed that the crude extract was able to inhibit the growth of all tested cell lines moderately with IC 50 values ranged from 10.7±0.05 to 12.4±0.10 µg/mL (p<0.001), respectively (Table 2) . Doxorubicin (IC 50 4.3, 3.8, 3.4 µg/mL, respectively) was used as a positive control (Table 2) . To gain more insight into the effect of encapsulation within the liposomal formulation on the improvement of the antiproliferative activity of the components in Coscinoderma sp. crude extract, MTT assay was carried out for the extract-containing liposomes. The IC 50 against HepG2, MCF-7, and Caco-2 cell lines was determined for the three investigated cell lines. Results show that the sensitivity of the three investigated cell lines was significantly enhanced after liposomal formulation. Where IC 50 of the crude extract-containing liposomes against HepG2, MCF-7, and Caco-2 has significantly decreased to 2.2±0.31, 4.1±0.25, and 1.7±0.18µg/mL, respectively (p<0.001). Cell viability of the three investigated cell lines was evaluated for Coscinodermacontaining liposomes (at IC 50 ) and their corresponding empty liposomes to exclude the cytotoxic effect of the phospholipid membrane ( Figure 10 ). This is consistent with previous studies that reported the impact of nanocarriers on the enhancement of the cellular uptake and accessibility of the entrapped cargo. 38-40 Nanomaterials with smaller particle sizes are easier to be up taken via endocytosis. 41 Since the low water solubility of extract components can be an obstacle against availability for absorption and cellular uptake, 42,43 enhancement of solubilization of the extract components, achieved by encapsulation, may have an important role in the improved cytotoxic effect against the cell lines under investigation. 44 Favored uptake by interstitial leaky vasculature of tumor tissues can be another scenario for the accelerated cellular internalization. 45 Besides, the presence of cholesterol contributes to the cellular uptake of liposomes. 46 Clinically, formulating such cytotoxic payload into a nano-carrier system, that would entrap both the hydrophilic and the lipophilic components with the improvement of cellular uptake, would be of great therapeutic value especially if designed as a longcirculating formulation, which is the scope of our upcoming work. In the present study, the metabolomic profiling of Coscinoderma sp. crude extract dereplicated 20 compounds, utilizing LC-HRESIMS. The identified metabolites were fit to diverse chemical classes, covering sponging diterpenes, an indole alkaloid, sesterterpenoid, sterol, and methylherbipoline Notes: The IC 50 value of compounds against each cancer cell line, which was defined as the concentration (µg/mL) that caused a 50% inhibition of cell growth in vitro, data were expressed as mean±SEM (n = 3). One-way analysis of-variance (ANOVA) followed by Dunnett's test using PASW Statistics ® version-18 (Quarry Bay, Hong Kong) was applied. GraphPad Prism software version-6 (La-Jolla, CA, USA) was used for statistical calculations. *Statistically significant at p < 0.001. Doxorubicin a positive control. International Journal of Nanomedicine 2021:16 https://doi.org/10.2147/IJN.S310720 Salt. Coscinoderma sp. crude extract showed moderate antiproliferative activities against HepG2, MCF-7, and Caco-2. The improved delivery to the studied cell lines was achieved by the entrapment of Coscinoderma sp. crude extract within liposomal vesicles. Although our results mainly denote the in vitro MTT experiments, liposomal entrapment of the extract seems to be a promising approach to enhance the antiproliferative potential of the extract components. PASS in silico predicted compounds 1-15 as antiproliferative which target both SHP2, and Pin-1. Further isolation of the active components from the crude extract together with the in vivo studies are in progress to find out the applicability of such formulation as an anticancer therapeutic approach. Coastal habitats and their importance for the diversity of benthic communities: a species-and trait-based approach Cytotoxic effects of Sarcophyton sp. soft corals-Is there a correlation to their NMR fingerprints? 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The manuscript management system is completely online and includes a very quick and fair peer-review system The writers would prefer to give their heartfelt gratitude to the central laboratory at Jouf University to aid this research. The writers give their gratitude to the Deputyship for Research and Innovation, Ministry of Education in Saudi Arabia for supporting this work through the project number "375213500". The authors declared no conflict of interest for this work.