key: cord-0063288-bsmn1gfw authors: Sun, Xuejun; Xie, Naxin; Guo, Mengling; Qiu, Xuelian; Chen, Hongwei; Liu, Haibo; Li, Hongmu title: Establishment of a Nomogram for Predicting Early Death in Viral Myocarditis date: 2021-05-12 journal: Cardiol Res Pract DOI: 10.1155/2021/9947034 sha: 27aff3546219603ccdf7a54fd812646161e96c6d doc_id: 63288 cord_uid: bsmn1gfw OBJECTIVE: This research aimed to establish a nomogram for predicting early death in viral myocarditis (VMC) patients. METHOD: A total of 362 consecutive VMC patients in Fujian Medical University Affiliated First Quanzhou Hospital between January 1, 2009, and December 31, 2019, were included. A least absolute shrinkage and selection operator (LASSO) regression model was used to detect the risk factors that most consistently and correctly predicted early death in VMC. The performance of the nomogram was assessed by calibration, discrimination, and clinical utility. RESULT: 9 factors were screened by LASSO regression analysis for predicting the early death of VMC. Combined with the actual clinical situation, the heart failure (HF) (OR: 2.13, 95% CI: 2.76–5.95), electrocardiogram (ECG) (OR: 6.11, 95% CI: 1.05–8.66), pneumonia (OR: 3.62, 95% CI: 1.43–9.85), brain natriuretic peptide (BNP) (OR: 4.66, 95% CI: 3.07–24.06), and lactate dehydrogenase (LDH) (OR: 1.90, 95% CI: 0.19–9.39) were finally used to construct the nomogram. The nomogram's C-index was 0.908 in the training cohort and 0.924 in the validation cohort. And the area under the receiver operating characteristic curve of the nomogram was 0.91 in the training cohort and 0.924 in the validating cohort. Decision curve analysis (DCA) also showed that the nomogram was clinically useful. CONCLUSION: This nomogram achieved an good prediction of the risk of early death in VMC patients. Viral myocarditis (VMC) is one of the common clinical cardiovascular diseases, which is caused by viral infection, especially the localized or diffuse myocardial inflammatory lesions caused by Coxsackie B virus [1] . e potential pathogenesis was considered to be that the virus-mediated immune response can directly act on cardiomyocytes and intracardiac capillaries, leading to degeneration and necrosis of cardiomyocytes and ultimately injury cardiac dysfunction [2, 3] . e prognosis of most cases is good, but a small number of patients can have an acute outbreak leading to heart failure or sudden death, a small number of patients keep the heart cavity enlarged for several months to several years without heart failure, or the condition deteriorates again and evolves into dilated myocarditis [4, 5] . At present, there is no uniform standard for the diagnosis of viral myocarditis. Due to the lack of specificity in the clinical manifestations of viral myocarditis and most auxiliary examinations, the pathogenesis is not fully understood and the treatment is not satisfactory [6, 7] . ere are no effective and safe preventive measures against early death from viral myocarditis. erefore, accurate prognostic assessment will help doctors understand the early death risk of patients with viral myocarditis and take timely intervention measures to increase the survival probability of patients. However, there is no predictive model with good predictive ability for early death in VMC patients till now. In this study, we retrospectively analyzed the existing case data to find the key factors for early death of viral myocarditis, build a nomogram to evaluate the severity of the patient's condition at the time of admission, and adopt corresponding treatment methods to reduce early death. e study was approved by the Ethics Committee of Quanzhou First Hospital Affiliated to Fujian Medical University. A total of 362 consecutive hospitalizations of patients who were clinically diagnosed with VMC from January 1, 2009, to December 31, 2019, were included. e patients included in the study must be finally diagnosed with VMC, referring to the diagnostic guidelines of the Chinese Medical Association [8, 9] , and the case data are complete. According to the clinical outcome at the time of discharge, they are divided into the survival group and death group ( Figure 1 ). All statistical analysis was performed with R Studio software (Version 3.6.3, https:// rstudio.com/). e least absolute shrinkage and selection operator (LASSO) regression method was used to detect the related risk factors in VMC [10, 11] . e "rms" package for R was used to make the nomogram. e accuracy of the nomogram was assessed by the discrimination ability and the calibration plot in the training set and the validating set [12] . AUC of receiver operating characteristic (ROC) was used to evaluate the discrimination ability of the nomogram. Decision curve analysis (DCA) was performed to evaluate the clinical utility of the nomogram [13, 14] . All tests were two-tailed, and p < 0.05 was considered statistically significant. A total of 362 VMC patients visiting our clinic from January 1, 2009, to December 31, 2019, were assigned to training cohort. According to the principle of random allocation, 254 cases were screened for eligibility as the training cohort. Another 94 patients were enrolled into as validation cohort. ere was no significant difference in demographic and clinical characteristics between the two groups, as given in Table 1 . LASSO regression analyzed was performed to reduce 26 variables to 9 potential predictors in the training cohort (Figures 2(a) and 2(b)). However, only 5 predictors were enrolled into nomogram after combined with the actual clinical situation, including HF (OR: 2.13, 95% CI: 2.76-5.95), ECG (OR: 6.11, 95% CI: 1.05-8.66), pneumonia (OR: 3.62, 95% CI: 1.43-9.85), BNP (OR: 4.66, 95% CI: 3.07-24.06), and LDH (OR: 1.90, 95% CI: 0. 19-9.39 ). e results of multivariate logistic analysis are presented in Table 2 . A nomogram was constructed for predicting early death in VMC patients ( Figure 3 ). Validation of the nomogram was performed with a 1000 bootstrap analysis. Harrell's concordance index was 0.908 in the training cohort and 0.924 in the validation cohort. e calibration curves of the nomogram showed good probability consistencies between the prediction and observation in the training cohort and validating cohort (Figures 4(a) and 4(b) ). e area under ROC curve of the probability of early death was 0.91 in the training cohort and 0.92 in the validating cohort (Figures 4(c) and 4(d) ). Decision curve analysis (DCA) was used to evaluate the clinical value of the nomogram using the data from all 362 patients. e DCA curve for the predictive nomogram is shown in Figure 5 , which shows that when the nomogram-predicted probability of early death was <66%, the nomogram provided additional value relative to the treat-all-patients scheme or the treatnone scheme, suggesting that the nomogram was clinically useful. Viral myocarditis (VMC) is caused by various viruses infecting the myocardium, most of which have a good prognosis [3] . However, it has been linked as the cause of sudden cardiac death in young adults in up to 12% of cases [15, 16] . Little is known about the early stages of VMC in humans currently [7, 17] . ere is no model with good predictive ability for early death in VMC patients till now. In the present study, a nomogram was established to predict early death based on the clinical features of patients with VMC. 5 predictors were used to establish the nomogram, which included HF, ECG, pneumonia, BNP, and LDH. Finally, the nomogram provided good discrimination and calibration values, which may help to timely intervene in patients with VMC who are at high risk of early death. HF is a consequence of various cardiovascular diseases, always associated with poor prognosis [18] . In this study, VMC patients with heart failure had a higher probability of early death. In addition, abnormal ECG is a strong prognostic indicator in the nomogram. However, Dec et al. suggested that ECG findings are neither sensitive nor specific for the diagnosis of myocarditis [19] , which requires further exploration. Zhang et al. [20] suggested that levels of brain natriuretic peptide (BNP) measured in the plasma could be a useful biochemical marker for the myocarditis, and high concentration of BNP may correlate with poor prognosis in patients with myocarditis. us, pneumonia and LDH elevated seemed to be an important sign of early death in VMC patients. However, no correlation with prognosis was found in previous studies. In this study, the levels of CK, CK_MB, and TNI_I were not significant for early death. However, a significant increase in TNI_I may suggest a worse prognosis [21] . ere are also several limitations of this research. First, this study was dependent on the data of a single institutional cohort of patients from the Asia-Pacific region. Second, this retrospective study was susceptible to certain biases that could not be completely avoided. ird, in medical practice, instead of applying endomyocardial biopsy (EMB), the current gold standard for diagnosis [22] , physicians rely on a combination of clinical features, laboratory analyses, and imaging to diagnosis VMC. In conclusion, we established and validated a nomogram for predicting early death in VMC patients. e nomogram has an adequate ability of discrimination, calibration, and may be a valuable tool for clinical practice. e data used to support the findings of this study are included within the article and within the supplementary files. e authors declare that they have no conflicts of interest. 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Supplementary Xuejun Sun and Naxin Xie contributed equally to this work. Xuejun Sun, Haibo Liu, and Hongmu Li contributed to the study design. Xuejun Sun, Naxin Xie, Mengling Guo, Hongwei Chen, and Xuelian Qiu contributed to literature search. Mengling Guo and Xuelian Qiu contributed to collect data. Xuejun Sun and Naxin Xie wrote the article and performed data analysis. Haibo Liu and Hongmu Li contributed to edit, supervision, and funding acquisition. All authors gave the final approval of the version to be submitted.