key: cord-0061766-p8oes0h4 authors: Fathi, Yaser; Hoseini, Elaheh Ghasemzadeh; Atoof, Fatemeh; Mottaghi, Reza title: Xerostomia (dry mouth) in patients with COVID-19: a case series date: 2021-04-06 journal: nan DOI: 10.2217/fvl-2020-0334 sha: 1cfd017e89dc6e4b18c9f81981efd98a094c838f doc_id: 61766 cord_uid: p8oes0h4 Introduction: Dry mouth has been reported as a symptom of COVID-19. In this study, xerostomia (dry mouth) was reported in patients with COVID-19. Materials & methods: Dry mouth was assessed in hospitalized patients with COVID-19 daily until all of the dry mouth symptoms resolved. Results: Dry mouth appeared in 60% of cases 3–4 days before as prodromal symptom and in others, simultaneously or 1–2 days after the onset of other symptoms. In most cases, with starting the treatment, dry mouth gradually disappeared. Conclusion: Xerostomia in COVID-19 could occur before the common symptoms. Therefore, it could be hypothesized that it could be used for early diagnosis, quarantine and treatment. As a result, disease transmission might be prevented and the best treatment outcomes could be achieved. Patients with COVID-19 were admitted to the Infectious Diseases Department of Shahid Beheshti Hospital in Kashan, Iran from mid-May to the end of June 2020. About 60% of the patients had dry mouth, which was more than the reported statistics in other studies (about 46%) [3] . In the present case series study, 10 patients with COVID-19 who had been examined in this center during this period and complained of dry mouth have been studied. The patients in this manuscript have given written informed consent to the publication of their case details and was reported after approval from the ethical committee in Kashan University on 11 August 2020 (ID: IR.KAUMS.REC.1399.025). Information on demographic characteristics, medical history, symptoms of the disease, diagnostic test results and treatment regimen of each patient were extracted from their hospital records. The state and severity of dry mouth were assessed via the visual analog scale (VAS) and were registered daily until all of the dry mouth symptoms resolved. To this end, patients were asked to rate the severity of dry mouth using a scoring system from 0 (for the absence of dry mouth) to 10 (for the most severe case of dry mouth). Thus, the changes in dry mouth symptoms were monitored and evaluated during the disease. Demographic, diagnostic and therapeutic data of patients are summarized in Table 1 . Out of ten cases, five were female and five were male. The mean age of the subjects was 42.63 years, with the oldest being 49 years old and the youngest 19 years old. Three of the subjects had gastrointestinal disorders and used proton pump inhibitors. Further, one of the subjects had an allergy and was taking antihistamines including hydroxyzine. One of the patients was a heavy smoker. However, all of these patients had never experienced dry mouth and had no complaints of symptoms before developing COVID-19 (it was the first time they had complained of dry mouth). Other patients had no medical history and did not take any specific medication. The most common symptoms at the time of admission were fever, cough, shortness of breath, fatigue and lethargy, although gastrointestinal symptoms and loss of taste were also reported in two cases. Diagnostic tests for COVID-19 included RT-PCR and CT scan of the lungs was carried out at the time of admission for all patients. The patients' treatment regimens were comparatively similar and included antiviral drugs, hydroxychloroquine, antibiotics and oxygen supplementation. Based on their medical history, none of the patients maintained a history of radiotherapy or treatment with radioactive iodine of head and neck. Based on the information collected via the monitoring of dry mouth symptoms, four patients encountered slight dry mouth (VAS = 1-3), four patients experienced moderate dry mouth (VAS = 4-7), and only two patients experienced severe dry mouth (VAS = 8-10). Symptoms of dry mouth appeared in six patients (60% of cases) 3-4 days before the general infection symptoms such as fever and respiratory complications. In other patients, dry mouth was observed simultaneously or 1-2 days after the onset of other symptoms (including cough and shortness of breath). In patients who had started with general symptoms such as fever and respiratory symptoms, dry mouth symptoms such as dysphagia, foamy saliva, and dry lips were often observed at higher degrees of VAS. In all cases, with starting the treatment and recovery, the symptoms of dry mouth gradually decreased. Moreover, in most cases (eight patients), it completely disappeared after a few days (2-13 days). Only two patients still displayed slight degrees of dry mouth after almost three weeks (VAS = 1-2). In our hospital setting, negativization of PCR test was not considered as a criterion for the patient's remission and discharge. Most of the patients were not tested again to investigate the negativization. Therefore, no data about oral indicators after negativization of PCR are provided. We recommend a comparison of VAS or xerostomia inventory between patients during pathology and the same patients after 1 month of negativization of RT-PCR. It should be useful to analyze the p-value. A total of 10 COVID-19 patients with signs of xerostomia were evaluated in the present study. As mentioned earlier, none of the cases had experienced the symptoms of xerostomia before. In addition, none of the risk factors of xerostomia were noted in the medical history of these people. It was surprising that xerostomia in approximately 60% of the patients in our study began 1-4 days prior to hospitalization and receiving COVID-19 medications. Freni et al. reported the signs of xerostomia in 32% of the COVID-19 patients in their investigation. These researchers demonstrated that in most of the cases, xerostomia occurred before other symptoms of the disease, and the severity of this problem diminished after 15 days [5, 6] patients their first-time experiencing xerostomia in their lifetime. According to this study xerostomia was reported as one of the first symptoms in 19.6% of patients with SARS-CoV-2 infection with a median onset time of 7 days (range: 4 to 7.8) before the COVID-19 diagnosis [6] . According to researches, SARS-CoV-2 uses ACE 2 receptors to enter the cells and utilizes the transmembrane serine protease Type 2 protein (TMPRSS 2) for priming [7] . The upregulation of these two receptors leads to the activation of the ribosomal pathway and the synthesis of viral RNA and proteins [8] . The ACE 2 and TMPRSS 2 receptors have a remarkable expression in the epithelial cells of salivary glands. Therefore, it seems that these glands are suitable receptors for SARS-CoV-2 and are among the first target cells of this virus and probably the virus can easily enter the salivary glands resulting in infection [8] . Different studies indicated that the SARS-CoV-2 virus is present in the whole saliva in the primary stages of the disease and is even detectable in the saliva secreted from salivary gland ducts after several days [3] . Liu et al. revealed that the SARS-CoV load was higher in saliva samples than blood specimens 48 h after intranasal viral challenge [9] . Considering the aforementioned data, virus penetration into salivary glands affects the function of these glands in the initial stages of the disease causing changes in saliva flow and components [3, 10] . One of the theories proposed for the latter alterations is the neurotropic role of SARS-CoV-2 [5] . Coronavirus could enter the CNS through intranasal and peripheral nerves. Neurones and neuroglia; neural cells that express the entry protein ACE2 can be affected by coronavirus. Therefore, even occurrence of a neurological disease is possible. Furthermore, the involvement of SARS-CoV-2 in CNS infections was underscored by the findings that made use of transgenic mouse models expressing the human ACE2, which is the cellular receptor used by the SARS-CoV-2 to infect susceptible cells. The virus enters peripheral nerves and spreads to the CNS through synaptic contacts. Sensory and autonomic innervation of parotid gland and parasympathetic innervation of submandibular glands can be affected [5] . ACE2 receptors have been found in the epithelium of taste buds and salivary glands, not only in rhesus macaques but also in humans. Salivary glands in rhesus macaques have been demonstrated to be an early target for SARS-CoV-2 and SARS-CoV RNA has been demonstrated to be present in saliva before pulmonary lesions [11] . According to the literature, the neuropathic and mucotropic effects of this virus can potentially affect the function of salivary glands and lead to hyposalivation and xerostomia [12] . Moreover, inflammatory and infectious procedures have been noted as factors influencing reduced saliva. As a result, the possibility of quantitative and qualitative salivary disorders due to SARS-CoV-2 infection in the salivary gland should be taken into consideration [13] . Consuming numerous medicines and diverse pharmacological groups is among other reasons suggested for xerostomia in COVID-19. The most common medications in patients with COVID-19 include antiviral agents (remdesivir), hydroxychloroquine, anti-HIV medications (ritonavir, lopinavir) and interferons [12] . Overall, medications are the most frequent reason for xerostomia. Among the common medicines for COVID-19 treatment, lopinavir, ritonavir and interferons play a remarkable role in xerostomia. Studies showed that the xerogenic effects of some of these medications remain even after complete recovery from the disease [12] . However, we mentioned earlier that the signs of xerostomia in COVID-19 patients usually occur before other symptoms and treatment initiation and decrease gradually during the recovery period. The latter point reduces the possibility of the role of medicines in generating xerostomia. It was also notable in our study that the signs of xerostomia began simultaneously or a little after the incidence of COVID-19 symptoms in most cases (60%). Lechien et al. investigated three cases of parotitis due to COVID-19 and mentioned lymphadenitis in the parotid gland as the reason. These authors demonstrated that elevation in parotid volume might result in the obstruction of Stensen duct, saliva retention and xerostomia due to hyposalivation [14] . However, none of the cases reported in the current study had signs of parotitis, such as a large parotid gland and the resultant obstruction of the salivary gland duct. Consequently, the exclusive report of dryness feeling by the patients is consistent with our results supporting the theory that the initial site of virus impact is salivary gland cells. Knight et al. indicated that congestion and rhinorrhea in patients with COVID-19 could lead to mouth breathing accompanied by sequels, such as xerostomia [15] . Anxiety and worries related to being affected by disease and hospitalization might be considered as background factors for xerostomia in patients [15] . In the current investigation, as mentioned earlier, 60% of the cases had the feeling of dry mouth without other common symptoms and this early onset of xerostomia was interesting. One of the oral manifestations in COVID-19 patients is xerostomia or dry mouth. Although diverse reasons have been noted for xerostomia in these cases, it seems that the presence of SARS-CoV-2 virus in salivary glands and the 10 .2217/fvl-2020-0334 Future Virol. (Epub ahead of print) future science group resultant alterations in these glands could be the most important reason for this sign. Xerostomia usually occurs a little before the common symptoms of the disease, namely fever, cough and dyspnea. Therefore, it could be hypothesized that this sign could be used for early diagnosis, quarantine and treatment of the patients. As a result, disease transmission might be prevented and the best treatment outcomes could be achieved. • SARS-CoV-2 could travel to other tissues such as salivary glands and make some alterations to them resulting in oral manifestations. • Xerostomia might be the initial manifestation of COVID-19 before signs and symptoms in the respiratory system. • It is prudent to consider COVID-19 as a differential diagnosis in case of xerostomia. • Saliva can be used for detection of SARS-CoV-2. • Xerostomia symptom might be useful for early diagnosis, quarantine and treatment of the patients with SARS-CoV-2. Financial & competing interests disclosure Xerostomia of various etiologies: a review of the literature Burket's Oral Medicine. PMPH Xerostomia-related quality of life for patients with oropharyngeal carcinoma treated with proton therapy World Health Organization. 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Oral Pathol The impact of coronavirus infectious disease 19 (COVID-19) on oral health Salivary glands, saliva and oral findings in COVID-19 infection Parotitis-like symptoms associated with COVID-19 The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.English Edit Institute editing services were utilized in the production of this manuscript. The authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.