key: cord-0028938-48lqvor6
authors: Hardan, Louis; Bourgi, Rim; Cuevas-Suárez, Carlos Enrique; Lukomska-Szymanska, Monika; Cornejo-Ríos, Elizabeth; Tosco, Vincenzo; Monterubbianesi, Riccardo; Mancino, Sara; Eid, Ammar; Mancino, Davide; Kharouf, Naji; Haikel, Youssef
title: Disinfection Procedures and Their Effect on the Microorganism Colonization of Dental Impression Materials: A Systematic Review and Meta-Analysis of In Vitro Studies
date: 2022-03-16
journal: Bioengineering (Basel)
DOI: 10.3390/bioengineering9030123
sha: 2b4deeabd98d0c9b3e8c092a9b5b693464f80744
doc_id: 28938
cord_uid: 48lqvor6

Dental impressions are contaminated with potentially pathogenic microorganisms when they come into contact with patient blood, saliva, and plaque. Numerous disinfectants are used; however, no sole disinfectant can be designated as universal for all the impression materials. Thus, the aim of this study is to systemically review the literature to evaluate the effect of the existing disinfection procedures on the bacterial colonization of dental impression materials. This systematic review and meta-analysis was conducted according to the PRISMA statement. PubMed (MEDLINE), Web of Science, Scopus, EMBASE, and SciELO databases were screened up to April 2021. Eligibility criteria included in vitro studies reporting the antibacterial activity of disinfectant solutions in dental impression materials. The meta-analysis was performed using Review Manager (version 5.3.5). A global comparison was performed with the standardized mean difference based on random-effect models at a significance level of α = 0.05. A total of seven studies were included in the meta-analysis. The included studies described the effect of disinfection processes with chlorhexidine gluconate, alcohol, sodium hypochlorite, glutaraldehyde, and hydrogen peroxide in alginate, polyvinyl siloxane, and polyether impression materials. The meta-analyses showed that the use of chlorhexidine, alcohol, glutaraldehyde, and sodium hypochlorite reduced the colony-forming units by a milliliter (CFU/mL) in alginate (p < 0.001). On the other hand, glutaraldehyde, sodium hypochlorite, and alcohol reduced the CFU/mL in polyvinyl siloxane (p < 0.001). Finally, alcohol and glutaraldehyde reduced the CFU/mL in polyether material (p < 0.001). High heterogenicity was observed for the alginate and polyvinyl siloxane materials (I(2) = 74%; I(2) = 90%). Based on these in vitro studies, the disinfection of impression materials with several disinfection agents reduces the CFU/mL count.

Dental impressions are certainly contaminated with possibly pathogenic microorganisms when they come into contact with patient blood, saliva, and plaque [1, 2] . This could be the source of disease transmitters and cross-infections for dentists, dental assistants, and laboratory technicians [3, 4] . Consequently, sanitizing the impressions efficiently before transportation to the laboratory technician ensembles is crucial [5] . Indeed, when the impressions are sterilized, this can avoid the transmission of disease, yet it is not the ideal way, since dimensional changes can occur [6] .

Considering that, in some countries, tap water contains halogenated compounds, the Advisory British Dental Association Service recommends the rinsing of impression materials with tap water in daily dental practice; despite this, although some of the microorganisms adhered to the surface of a dental impression could be removed by this procedure, a high percentage still remains [7] . This has been exhibited to lessen the amounts of the bacteria on the surface of the impression presented by nearly 90% [8] . Nevertheless, a noteworthy number of bacteria would persist. More recent suggestions support the use of a disinfecting solution [9] . Knowledge evidently varies about the type, concentration, and immersion times of disinfection protocols, making it difficult to evaluate the most applicable method [10, 11] .

Numerous disinfectants are used regularly such as sodium hypochlorite, chlorhexidine, alcohol, glutaraldehyde, and hydrogen peroxide [12] . Since no sole disinfectant can be designated as a universal disinfectant for all impression materials, it is fundamental to select an ideal disinfectant agent with superior antimicrobial activity that does not disturb the recorded features, such as surface characteristics or dimensional stability of an impression materials [13, 14] .

Additionally, many combinations between impression materials and disinfectant could occur by knowing that a large range of branded impression materials (reversible and irreversible hydrocolloids, polyethers, polysulphides, and silicones) and gypsum-based casts existed in the marketplace. A disinfectant possesses a dual purpose: it needs to be an effective antimicrobial agent but produce no adverse effect on the dimensional accuracy of the impression material and resultant gypsum model. The latter is of significance in an attempt to deliver a functional and well-fitting finished appliance. Disagreement happens in the literature as to whether the disinfection procedure produces degradation or distortion of impressions [15] [16] [17] .

The reaction of some specific brands of gypsum products and impression materials to disinfection process is diverse, advising a deficiency of compatibility between a given material and protocol. Hence, individual analysis of impression materials is needed to define the effectiveness of a specific disinfection method in different areas [18] .

Accordingly, the aim of this study was to systemically review the literature of the existing disinfection procedures on the bacterial colonization of dental impression materials.

The null hypothesis to be tested was that the use of disinfectant agents will not reduce the colony-forming units per milliliter (CFU/mL) adhered to the surface of impression materials used in dentistry.

This systematic review and meta-analysis was reported following the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA statement) [19] . The registration protocol was carried out in the Open Science Framework with the registration number 0000-0002-2759-8984. The following PICOS strategy was used: population, impression materials; intervention, use of disinfection materials; control, rinsing with tap water; outcome: antimicrobial activity; and type of study, in vitro studies. The research question was as follows: Does the use of disinfection procedures for impression materials in dental practice reduce the microbial count?

The literature search was performed by two independent reviewers (E.C.R. and R.B.) up to April 22, 2021 . The following databases were screened: PubMed (MEDLINE), Web of Science, Scopus, EMBASE, and SciELO. The search strategy was performed according to the keywords defined in Table 1 . All studies were imported into Rayyan QCRI platform [20] . 

The title and abstract of each identified article were reviewed by two independent reviewers (E.C.R. and R.B.) to determine if the article should be considered for full-text review according to the following eligibility criteria: (1) in vitro studies reporting the antibacterial activity of disinfectant solutions in dental impression materials; (2) included mean and standard deviation (SD) in CFU/mL; (3) included a control group where tap water was used; and (4) published in the English language. Case reports, case series, pilot studies, expert opinions, conference abstracts, and reviews were excluded. In the case of disagreements at the time of the selection of the studies for the full-text review, they were resolved by discussion and consensus by a third reviewer (C.E.C.-S).

The Microsoft Office Excel 2019 program (Microsoft Corporation, Redmond, Washington, DC, USA) was used to extract the data of interest from the included manuscripts. These were placed on a standardized form. Two reviewers (L.H. and R.B.), who received training in this software, performed the analysis. The data recovered from each manuscript were author, year, impression material evaluated, disinfection agents used, type of microorganism evaluated, main outcome, and main results.

The risk of bias of the selected articles was assessed by two reviewers (R.B. and E.C.R.) according to the parameters of the previous systematic review [21] . The risk of bias of each article was evaluated according to the description of the following parameters: specimen randomization, single-operator protocol implementation, blinding of the operator, the presence of a control group, complete outcome data, and description of the sample size calculation. If the authors reported the parameter, the study received a "YES" for that specific parameter. In case of missing information, the parameter received a "NO". The risk of bias was classified according to the sum of "YES" answers received: 1 to 2 indicated a high bias, 3 to 4 indicated a medium risk of bias, and 5 to 6 indicated a low risk of bias.

The meta-analyses were performed using Review Manager Software version 5.1 (The Nordic Cochrane Centre, The Cochrane Collaboration, Copenhagen, Denmark). The analyses were carried out using a random-effect model, and pooled-effect estimates were obtained by comparing the standardized mean difference between CFU/mL values obtained when a disinfection agent was used; against a control group when tap water was used. The standardized mean difference was performed since this statistic in meta-analysis is used when all the studies assess the same outcome but measure it in a variety of ways; for this to be appropriate, it must be assumed that between-study variation reflects only differences in measurement scales, such as the different scientific notation used among the studies included. Additionally, for comparison purposes, when a value of 0 was found in the data, this was replaced with "0.1" with a SD of "0.01" for the statistical analysis. The comparisons were made considering the type of impression material and the type of disinfection agent used. A p-value < 0.05 was considered statistically significant. Statistical heterogeneity of the treatment effect among studies was assessed using the Cochran Q test and the inconsistency I 2 test.

The search resulted in the retrieval of 2598 records ( Figure 1 ). After removal of duplicates, 2084 articles were screened, and 2027 were excluded based on the title or abstract. A total of 57 full-text articles were assessed for eligibility. Of these, nineteen were not considered for the qualitative analysis: seventeen did not evaluate the antibacterial activity and two were short communications, leaving thirty-eight studies for the qualitative analysis; from these, thirty-one were excluded from the quantitative analysis: in fourteen studies, the SD could not be retrieved, and in another thirteen studies, the results were not expressed in CFU/mL, two studies did not have any control group, and two studies did not have enough comparison groups. Finally, seven studies were considered for the meta-analysis. Table S1 describes the quantitative data extracted from studies included in the meta-analysis.

The meta-analyses were performed using Review Manager Software version 5.1 (The Nordic Cochrane Centre, The Cochrane Collaboration, Copenhagen, Denmark). The analyses were carried out using a random-effect model, and pooled-effect estimates were obtained by comparing the standardized mean difference between CFU/mL values obtained when a disinfection agent was used; against a control group when tap water was used. The standardized mean difference was performed since this statistic in meta-analysis is used when all the studies assess the same outcome but measure it in a variety of ways; for this to be appropriate, it must be assumed that between-study variation reflects only differences in measurement scales, such as the different scientific notation used among the studies included. Additionally, for comparison purposes, when a value of 0 was found in the data, this was replaced with "0.1" with a SD of "0.01" for the statistical analysis. The comparisons were made considering the type of impression material and the type of disinfection agent used. A p-value < 0.05 was considered statistically significant. Statistical heterogeneity of the treatment effect among studies was assessed using the Cochran Q test and the inconsistency I 2 test.

The search resulted in the retrieval of 2598 records ( Figure 1 ). After removal of duplicates, 2084 articles were screened, and 2027 were excluded based on the title or abstract. A total of 57 full-text articles were assessed for eligibility. Of these, nineteen were not considered for the qualitative analysis: seventeen did not evaluate the antibacterial activity and two were short communications, leaving thirty-eight studies for the qualitative analysis; from these, thirty-one were excluded from the quantitative analysis: in fourteen studies, the SD could not be retrieved, and in another thirteen studies, the results were not expressed in CFU/mL, two studies did not have any control group, and two studies did not have enough comparison groups. Finally, seven studies were considered for the meta-analysis. Table S1 describes the quantitative data extracted from studies included in the meta-analysis. The characteristics of the studies included in this systematic review are summarized in Table 2 . Several disinfection agents were identified for the present review, including chlorhexidine, alcohol, sodium hypochlorite, glutaraldehyde, and hydrogen peroxide. Most of the studies included in this review evaluated the antibacterial activity to alginate and polyvinyl siloxane impressions, only two studies evaluated the effect of disinfection on polyether, while only one tested on condensation silicone. Utmost of the studies reported the effect of disinfection agents on CFU/mL, while a few reported inhibition halos. Figures 2-4 show the result from the meta-analyses. With regards to alginate, the use of disinfection agents such as chlorhexidine, alcohol, glutaraldehyde, and sodium hypochlorite significantly reduced the CFU/mL count (p < 0.001). It is worth mentioning that a high heterogenicity was observed (I 2 = 74%) (Figure 2 ). 4 show the result from the meta-analyses. With regards to alginate, the use of disinfection agents such as chlorhexidine, alcohol, glutaraldehyde, and sodium hypochlorite significantly reduced the CFU/mL count (p < 0.001). It is worth mentioning that a high heterogenicity was observed (I 2 = 74%) (Figure 2 ). With regards to alginate, the use of disinfection agents such as chlorhexidine, alcohol, glutaraldehyde, and sodium hypochlorite significantly reduced the CFU/mL count (p < 0.001). It is worth mentioning that a high heterogenicity was observed (I 2 = 74%) (Figure 2 ). Figure 3 shows the effect of different disinfection agents on polyvinyl siloxane material. According to the meta-analysis, all the disinfection agents tested significantly reduced the CFU/mL count (p < 0.001). Again, a high heterogenicity was observed in the comparisons (90%).

Finally, Figure 4 shows the effect of different disinfection agents on polyether impression material. According to the meta-analysis, both alcohol and glutaraldehyde significantly reduced the CFU/mL count (p < 0.001). As only one study was included in this analysis, a 0% heterogenicity was found.

The risk of bias analysis was shown that most of the studies were categorized with high and medium risk of bias ( Table 3) . Utmost of the manuscripts examined failed to report the single operator, operator blinded, and sample size calculation factors. Figure 3 shows the effect of different disinfection agents on polyvinyl siloxane material. According to the meta-analysis, all the disinfection agents tested significantly reduced the CFU/mL count (p < 0.001). Again, a high heterogenicity was observed in the comparisons (90%).

Finally, Figure 4 shows the effect of different disinfection agents on polyether impression material. According to the meta-analysis, both alcohol and glutaraldehyde significantly reduced the CFU/mL count (p < 0.001). As only one study was included in this analysis, a 0% heterogenicity was found.

The risk of bias analysis was shown that most of the studies were categorized with high and medium risk of bias ( Table 3) . Utmost of the manuscripts examined failed to report the single operator, operator blinded, and sample size calculation factors. 

This systematic review and meta-analysis was directed towards testing the effect of disinfection agents on the bacterial colonization of different impression materials. This review focused on the study of the CFU/mL measure, since this is the most common measure used to determine the antibacterial activity. To the best of the authors knowledge, this is the first approach to prove that the application of disinfectant agents is effective to reduce the count of some oral pathogens on the surface of alginate, polyvinyl siloxane, and polyether impression materials and that this procedure can certainly reduce the possibility of cross-contamination. Accordingly, the hypothesis tested in this study was rejected.

Normally, chemical disinfectant agents were generally used in dental exercise because of their easy application. For the alginate materials, the use of disinfection agents such as chlorhexidine, alcohol, glutaraldehyde, and sodium hypochlorite significantly reduced the CFU/mL count (p < 0.001). Irreversible hydrocolloids, the frequent material used in dentistry, tend to absorb both blood and saliva [23] . Thus, research was focused on a solution to inhibit the colonization of microbe on the surface of these materials [11] .

Collected data were established on the CFU in a media culture. These were recorded by using a colony counter, and the counts were expressed by a standard technique of estimating microbial colony count known as the CFU count. The bacteriological examination evidently exhibited that the CFU recorded after disinfection were fewer than before disinfection [23] , thus making the disinfection process an important issue to solve after taking an impression in the dental world.

It is highlighted in a previous study [58] that the use of tap water on the surface of alginate impression failed to kill Streptococcus Mutans and Lactobacilli; however, by using chlorhexidine, a positive antimicrobial activity has been shown [59] . This could be possible by the binding between the positive site of chlorhexidine and negative sites of the bacterial cell, which resulted in interference with osmosis and escapes the constituents that lead to cell death [22] . In addition, alcohol was able to kill all the detected bacteria in this study by inactivating the growth of the bacteria on the alginate impression, and this was deemed probable by alkylating the amino and sulf hydral groups of bacterial proteins [60] [61] [62] . Further, for the other disinfectants, it was demonstrated that by using 2% glutaraldehyde solution or 1% sodium hypochlorite, gram-positive organisms will be modified by reducing their growth [11] . Indeed, this effect was most noticeable for 1% sodium hypochlorite, as described in this research.

A previous study denoted that after immersion in sterile water for 10 min, for some of impression materials, including alginate impression, the number of microorganisms counted was diminished, though alginate material still retained some of these microorganisms in comparison to other materials [24] . The physical nature of alginate impression could affect the capacity of disinfectants for doing their biocidal activity. In the oral environment, microorganisms might become integrated into the gelling impression material since the presence of oral fluids or saliva [29] . The set-up of these microorganisms in the alginate material restricted the efficacy of the water rinse, and the alginate gel assembly could hinder the penetration of the disinfectant [29, 63] . Thus, this idea explained the results of this study as tap water did not reduce the microorganism counts in comparison to the other disinfectant solution. Overall, for alginate impressions, the use of disinfectant agents would be of great interest, and the efficacy of the disinfection ranged between 92% and 99.97% in all the situations [23] .

According to the meta-analysis, all the disinfection agents significantly reduced the CFU/mL count (p < 0.001) on polyvinyl siloxane material. Among the numerous available impression materials in prosthodontics, this material was considered the material of choice, due to their fine detail reproduction, excellent physical properties, remarkable dimensional stability, good acceptance by the patient, and elastic recovery feature [64] [65] [66] . In addition, these materials were tasteless and odorless [46] . As stated above in alginate impression, using 2% glutaraldehyde solution or 1% sodium hypochlorite could be recommended also for disinfecting the polyvinyl siloxane impression [67, 68] . In this manner, it is advisable to immerse these kinds of impressions in these solutions rather than spraying, as successful finding was observed in many previous studies, without harming the physical properties [15, 18, 31, 69] . Seemingly, by putting the polyvinyl siloxane material in an alcohol-based disinfectant solution for a contact time of 15 min, a media free of microorganisms could be observed [24] . Accordingly, this can support the finding obtained in this study, as any kind of disinfectant solution tested showed promising results with polyvinyl siloxane impression material.

The present analysis noted that both alcohol and glutaraldehyde significantly reduced the CFU/mL count (p < 0.001) of polyether impression material. This could be explained by the fact that the contaminating bacteria could be reduced by 85% when soaking this kind of impression material in sterile water for 15 min; in addition, as found with polyvinyl siloxane impression, the use of alcohol-based solution produced effective disinfection of the polyether impression [24] . With regards to glutaraldehyde, the antimicrobial activity of this compound depends on the duration of dilution and its concentration. This could be elucidated by the fact that the biocidal activity of glutaraldehyde results from alkylation of sulfhydryl, hydroxyl, carboxyl, and amino groups of microorganisms, which alters RNA, DNA, and protein synthesis [70] . This conclusion seems to support the results in this meta-analysis.

From this review, various disinfectant agents were used to show the importance of reducing microorganisms on the surface of the impression materials used in dentistry. The results should be considered with caution since other brands of impression materials were available in the dental market and not included. In addition, there is the opportunity for slight changes in chemistry of these materials, causing significantly different reactions. Additionally, most of the studies included were classified as having high or medium risk of bias, and, therefore, better experimental designs should be conducted in order to obtain a higher degree of evidence. One of the limitations of this review relies on the fact that it is only focusing on the antibacterial efficacy of the application of a disinfectant on the surface of a dental impression; however, other variables should be taken into account, such as the effect of this procedure on the accuracy, precision, and surface quality of the resulting working models, especially when dental impressions are disinfected both in the dental office and in the dental laboratory. This led to controversy as to whether the disinfection process causes degradation or distortion of dental impressions and to what extent. Therefore, studying the effect of these disinfectants on the dimensional stability of the impression materials should be considered in further research. Additionally, viruses could be considered in future investigation by having the required equipment since their manipulation was considered dangerous for some researchers. Moreover, clinical studies were needed since testing of the efficacy of disinfectants from different patients derived impressions was scarce, knowing the differences in oral flora composition of individual person.

Based on in vitro studies, disinfection of alginate with chlorhexidine, alcohol, glutaraldehyde, and sodium hypochlorite reduced the CFU/mL count on the surface of alginate impressions. This trend was observed when polyvinyl siloxane impressions were disinfected with glutaraldehyde, sodium hypochlorite, and alcohol and when polyether was immersed in alcohol or glutaraldehyde. Therefore, these substances could be employed to reduce cross-contamination in the dental office.

Comprehensive Review and Comparison of the Disinfection Techniques Currently Available in the Literature

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The Persistence of Microorganisms on Impression Materials Following Disinfection

Carriage of Oral Flora on Irreversible Hydrocolloid and Elastomeric Impression Materials

Disinfection of dental impressions: Knowledge and practice among dental technicians

Disinfection/Sterilization Protocols Recommended by Manufacturers of Impression Materials

British Dental Association. The Control of Cross-Infection in Dentistry

The effect of three different disinfectant materials on polyether impressions by spray method

A Survey of the Methods of Disinfection of Dental Impressions Used in Dental Hospitals in the United Kingdom

General Dentists' Knowledge about Disinfecting Dental Impressions

Disinfection Of Impression Material-A Review

Study of Knowledge of Hand Disinfection and Dental Impressions in Everyday Practice among Dental Students during a Pandemic by Coronavirus Disease 2019. Open Access Maced

Disinfection of Irreversible Hydrocolloid Impression Material with Chlorinated Compounds: Scientific

Infection Control Recommendations for the Dental Office and the Dental Laboratory

Effects of Disinfectants on Dimensional Accuracy of Impression Materials

Linear Dimensional Changes in Dental Impressions after Immersion in Disinfectant Solutions

Sodium Hypochlorite Disinfection of Irreversible Hydrocolloid Impression Material

Effect of Disinfectant Agents on Dimensional Stability of Elastomeric Impression Materials

The PRISMA 2020 Statement: An Updated Guideline for Reporting Systematic Reviews

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Effect of the Different Disinfectants on the Microbial Contamination of Alginate Impression Materials

Disinfection of Alginate and Addition Silicon Rubber-Based Impression Materials

Antimicrobial Effect of 4 Disinfectants on Alginate, Polyether, and Polyvinyl Siloxane Impression Materials

Comparison of Antimicrobial Activities and Compressive Strength of Alginate Impression Materials Following Disinfection Procedure

A Simple and Effective Method for Addition Silicone Impression Disinfection

Efficacy of Various Disinfecting Agents on the Reduction of Bacteria from the Surface of Silicone and Polyether Impression Materials

Evaluation of Antibacterial Effect and Dimensional Stability of Self-Disinfecting Irreversible Hydrocolloid: An in Vitro Study

Immersion Disinfection of Irreversible Hydrocolloid Impressions with Sodium Hypochlorite. Part I: Microbiology

In Vitro and Clinical Examination of the Effect of.an Antimicrobial Impression Material on the Oral microflora

Effect of Inmersion Desinfection with 0.5% Sodium Hypochlorite and 2% Glutaraldehyde on Alginate and Silicone: Microbiology and SEM Study

Disinfectant Efficacy of 0.525% Sodium Hypochlorite and Epimax on Alginate Impression Material

Irreversible Hydrocolloids: A Comparison of Antimicrobial Efficacy

Antibacterial Efficacy and Effect of Chlorhexidine Mixed with Irreversible Hydrocolloid for Dental Impressions: A Randomized Controlled Trial

Effectiveness of Disinfectants on Antimicrobial and Physical Properties of Dental Impression Materials

Efficacy of Various Spray Disinfectants on Irreversible Hydrocolloid Impression Materials: An in Vitro Study

Disinfection of Bacterially Contaminated Hydrophilic PVS Impression Materials

Antimicrobial Activities of Dental Impression Materials

Factors Affecting the Stability of Sodium Hypochlorite Solutions Used to Disinfect Dental Impressions

Antimicrobial Activity and Properties of Irreversible Hydrocolloid Impression Materials Incorporated with Silver Nanoparticles

A Comparative Study between Microwave Irradiation and Sodium Hypochlorite Chemical Disinfection: A Prosthodontic View

Evaluation of Antimicrobial Effects on Dental Impression and Biofilm Removal by Sodium Dichloroisocyanurate

The Fungicidal Effect of Ultraviolet Light on Materials Impression

A Self-Disinfecting Irreversible Hydrocolloid Impression Material Mixed with Povidone Iodine Powder

Disinfection of Dental Stone Casts: Antimicrobial Effects and Physical Property Alterations

Comparative Evaluation of the Antimicrobial Efficacy of Three Immersion Chemical Disinfectants on Clinically Derived Poly (Vinyl Siloxane) Impressions

Radiofrequency Glow Discharge as a Mode of Disinfection for Elastomeric Impression Materials

Disinfection of Irreversible Hydrocolloid Impressions: A Comparative Study

Disinfection of Irreversible Hydrocolloid Impressions with Sodium Hypochlorite Steam: Assessment of Antimicrobial Efficacy

Incorporation of Disinfectants for Obtaining Dental Stone: Microbiological and Dimensional Evaluation

Efficacy of Different Disinfectant Systems on Alginate and Addition Silicone Impression Materials of Indian and International Origin: A Comparative Evaluation

Prevention of Cross-Contamination Risk by Disinfection of Irreversible Hydrocolloid Impression Materials with Ozonated Water

Immersion Disinfection of Irreversible Hydrocolloid Impressions in PH-Adjusted Sodium Hypochlorite. Part 1: Microbiology

Evaluation of the Efficacy of Different Mixing Techniques and Disinfection on Microbial Colonization of Polyether Impression Materials: A Comparative Study

Basic Properties of an Alginate Impression Material Supplemented with Chlorhexidine I. Disinfectant Effects on Oral Microbes

Evaluation of Efficacy of Aloe Vera as a Disinfectant by Immersion and Spray Methods on Irreversible Hydrocolloid Impression Material and Its Effect on the Dimensional Stability of Resultant Gypsum Cast-An in Vitro Study

Effect of Ultraviolet Radiation Combined with Immersion Disinfection of Silicone Impressions Infected with Hepatitis B Virus and HIV

Efficacy of Chlorhexidine in Reduction of Microbial Contamination in Commercially Available Alginate Materials-In-Vitro Study

The Efficacy of Povidone-Iodine Products against Periodontopathic Bacteria

Efficacy of Various Disinfectants on Bacterial and Fungal Contamination of Clamping Tweezers

The Effect of Various Disinfectants on Dental Shade Guides

Desident CaviCide a New Disinfectant

Coaxial Alginate Hydrogels: From Self-Assembled 3D Cellular Constructs to Long-Term Storage

Siloxane Impression Materials: A Review of Properties and Techniques

A Review of Contemporary Impression Materials and Techniques

Siloxane Impression Materials: An Update on Clinical Use

Mechanism of Action of Sodium Hypochlorite

Disinfection Procedures: Their Effect on the Dimensional Accuracy and Surface Quality of Irreversible Hydrocolloid Impression Materials and Gypsum Casts

In-Vivo Evaluation of Impression Material Disinfection with Different Disinfectant Agents

Buffered Glutaraldehyde-A New Chemical Sterilizing Solution