key: cord-0028641-vzth8t33 authors: He, Xiao-Qun; Li, Xian; Wu, Yan; Wu, Shun; Luo, Tian-You; Lv, Fa-Jin; Li, Qi title: Differential Diagnosis of Nonabsorbable Inflammatory and Malignant Subsolid Nodules with a Solid Component ≤5 mm date: 2022-03-11 journal: J Inflamm Res DOI: 10.2147/jir.s355848 sha: 765d9e7239c6b9c4852a5045bc18b473eff24a27 doc_id: 28641 cord_uid: vzth8t33 PURPOSE: To investigate the differential clinical and computed tomography (CT) characteristics of pulmonary nonabsorbable inflammatory and malignant subsolid nodules (SSNs) with a solid component ≤5 mm. PATIENTS AND METHODS: We retrospectively analyzed 576 consecutive patients who underwent surgical resection and had SSNs with a solid component ≤5 mm on CT images. These patients were divided into inflammatory and malignant groups according to pathology. Their clinical and imaging data were analyzed and compared. Multiple logistic regression analysis was used to identify independent prognostic factors differentiating inflammatory from malignant SSNs. Furthermore, 146 consecutive patients were included as internal validation cohort to test the prediction efficiency of this model. RESULTS: Significant differences in 11 clinical characteristics and CT features were found between both groups (P < 0.05). Presence of respiratory symptoms, distribution of middle/lower lobe, irregular shape, part-solid nodule (PSNs), CT value of ground-glass opacity (GGO) areas <−657 Hu, presence of abnormal intra-nodular vessel sign, and interlobular septal thickening were the most effective factors for diagnosing nonabsorbable inflammatory SSNs, with an AUC (95% CI), accuracy, sensitivity, and specificity of 0.843 (95% CI: 0.811–0.872), 89.76%, 72.86%, and 81.23%, respectively. The internal validation cohort obtained an AUC (95% CI), accuracy, sensitivity, and specificity of 0.830 (95% CI: 0.759–0.887), 83.56%, 73.91%, and 76.42%, respectively. CONCLUSION: Nonabsorbable inflammatory and malignant SSNs with a solid component ≤5 mm exhibited different clinical and imaging characteristics. Clinicians around the world frequently encounter pulmonary subsolid nodules (SSNs) during lung cancer screening using computed tomography (CT), which also poses a diagnostic challenge to radiologists. SSN is a morphological description for various pulmonary nodules, including pure ground-glass nodules (pGGNs) and part-solid nodules (PSNs), the latter of which contain both ground-glass opacity (GGO) and solid opacity. [1] [2] [3] [4] Previous studies have indicated that SSNs, particularly PSNs, are considered to have a higher potential for malignancy than do solid pulmonary nodules of a similar size. 2, 5 However, it is worth mentioning that SSN is a nonspecific finding, and some inflammatory entities, such as focal inflammation, focal fibrosis, and organizing pneumonia, can also manifest as this pattern. 6 Generally, most inflammatory SSNs may disappear on follow-up CT examinations. Therefore, it is usually recommended that patients with nonabsorbable SSNs receive surgical treatment after a period of follow-up CT. This may cause unnecessary surgery of nonabsorbable inflammatory SSNs. Ye et al 7 retrospectively evaluated 736 patients with SSNs undergoing a surgical procedure; of these SSNs, 7.4% turned out to be benign. 8 Some investigators have indicated that surgical operation may damage pulmonary function, even in cases of wedge or segmental resection. 9, 10 Hence, imaging differentiation of nonabsorbable inflammatory and malignant SSNs preoperatively is crucial in minimizing overtreatment and making optimal treatment decisions. Currently, CT is the preferred imaging modality for the screening and diagnosis of SSNs. 2015 WHO classification defined SSNs with invasive component ≤5 mm as preinvasive lesions and minimally invasive adenocarcinoma (MIA). 11 Previous studies have found a significant correlation between the size of the solid component on thin-section CT and the invasive component on pathology for SSNs. 12, 13 Some investigators in the field have proposed the development of clinical and CT guidelines for discriminating between inflammatory and malignant SSNs. However, to date, most studies have focused on the differential diagnosis between inflammatory and invasive SSNs, and few have sought to study the differential diagnosis between inflammatory and preinvasive or minimally invasive SSNs. Therefore, this study aimed to compare the clinical and CT features that distinguish nonabsorbable inflammatory SSNs, which was defined as nodules persistent for more than three months on follow-up CT without any decrease in size here, from malignant SSNs with a solid component ≤5 mm. Our hospital's institutional review board approved this study, and the need for informed consent was waived due to its retrospective nature. Meanwhile, this study was conducted in accordance with the Declaration of Helsinki, and used anonymous data and had no potential risks to patients. For this retrospective study, 604 consecutive patients between August 2018 and November 2020 were initially included. These patients fulfilled the following inclusion criteria:1) patients undergoing chest CT scan; 2) patients with a nodule manifesting as SSN with the largest solid-component dimension ≤5 mm in the lung window setting (window width, 1600 Hounsfield units; window level, −600 Hounsfield units); 3) SSNs persistent for more than three months on follow-up CT without any decrease in size; 4) nodules were surgically resected and confirmed pathologically. Twenty-eight patients were excluded because the size of the solid component within the SSN was difficult to measure accurately via CT. In total, 576 patients were finally included. These patients were divided into inflammatory and malignant groups based on pathological results. We also recorded patients' clinical characteristics, including age, sex, smoking history, and respiratory symptoms. Furthermore, 146 consecutive patients from December 2020 to February 2021 who met the inclusion and exclusion criteria were included as the internal validation cohort. Non-contrast chest CT examinations were performed using a Discovery 750 HD CT (GE Healthcare, Milwaukee, WI, USA); Somatom Perspective (GE Healthcare, Erlangen, Germany); or Somatom Definition FLASH (Siemens Healthcare, Forchheim, Germany) CT scanner. Patients were all placed in a supine position and scanned in the craniocaudal direction. CT scanning was first conducted at the end of inspiration during a single breath-hold, from the thoracic inlet to immediately below the costophrenic angle, with the following parameters: tube voltage of 110-120 kVp, tube current of 50-250 mAs (automatic tube current modulation technology), rotation time of 0.5 s, pitch of 0.875-1.50, slice thickness of 5 mm, and interval of 5 mm on axial images. Subsequently, images were reconstructed with a slice thickness of 0.625-1.25 mm and a slice interval of 0.625-1.25 mm, standard filtered back projection algorithm involving a kernel of high spatial resolution and displayed with a standard lung window setting. Two chest radiologists (with 20 and 13 years of experience in chest CT imaging, respectively) who were blinded to patient data interpreted the images together, and decisions on CT findings were reached through consensus. CT morphological features of each nodule were carefully analyzed as follows: 2. distribution (upper or middle and lower lobes) 3. shape (regular [round or oval] or irregular [with an uneven contour that could not be classified as round or oval] [ Figure 1A and B]) [ Figure 1A and B]) 5. intra-nodular characteristics, including density (pGGN and PSN [ Figure 1C and D]); CT value of GGO areas (measurement method: the largest slice of SSN for each patient was selected from axial CT images in the lung window. For pGGNs, a circular region of interest (ROI) was drawn to include more than two-thirds of the entire nodule, while for PSNs, a circular ROI as large as possible was placed on GGO regions of the nodule cautiously avoiding solid component, vessels, and bronchi. To reduce error, the same ROI was placed on three adjacent images using a copy-and-paste function, and the average values were calculated as CT value of GGO areas [ Figure 1C and D]); target sign (characterized by a focal and round-like solid component in the center of GGOpredominant nodule, similar to a target [ Figure 1E ]); bubble lucency (round or oval air attenuation within lesions [ Figure 1F ]); air bronchogram (branched or tubular air structure within lesions [ Figure 1G ]); intra-nodular vessel sign (vessels passing through nodules [ Figure 1H ]); and abnormal intra-nodular vessel sign (vessels in the nodule manifesting as dilatation, distorting, or increased branches. Dilatation was defined as increased vascular diameter by comparing with that of other vessels of the same grade in the identical or other lung lobes [ Figure 1I ]. Distorting was defined as curving of the vessels within the nodule. Increased branches were identified by comparing with those of adjacent vessels of the same grade). 6. Extra-nodular characteristics, including thickened interlobular septa [ Figure 1J ], pleural changes (pleural attachment [lesion attached to the pleura with the margin obscured by the pleura] [ Figure 1K ] and pleural retraction [linear structures connected between the tumor and pleura] [ Figure 1L ]). For patients with sequential CT scans, image analyses were based on data from the final CT scan taken before treatment commenced. All patients received sub-lobar resection or lobectomy. The specimens were fixed in 10% formalin, sliced at the maximum dimension, embedded in paraffin, sectioned with a microtome, and then stained with hematoxylin and eosin. A senior pathologist (X.L., with 13 years of experience in pathological diagnosis of the lung diseases) who was blinded to patient data evaluated all existing histopathological slides. Pathological analysis of nodules was identified according to the recently updated World Health Organization (WHO) classification (2015) and the eighth edition of the American Joint Committee on Cancer staging system for non-small cell lung cancer (2017). 12, 14, 15 Statistics Analysis Statistical analyses were performed using a statistical software package (SPSS, version 21.0 for Windows; IBM; NY, USA). Firstly, continuous variables were tested for normality using Kolmogorov-Smirnov analysis. Normally distributed quantitative data were expressed as mean ± standard deviation and analyzed using the Two-independent-samples Student's t-test, whereas non-normally distributed data were presented as median ± interquartile range and assessed using the Mann-Whitney U-test. Categorical variables were expressed as numbers and percentages and evaluated using the Chi-squared test. The Youden Index was calculated to establish the optimal threshold values of CT value of GGO areas. For comparison of clinical and CT features, a two-sided P value < 0.05 was considered to indicate a significant difference. Moreover, the diagnostic consistency was assessed using intraclass correlation coefficients (ICCs) for quantitative data and Kappa test for qualitative variables. ICCs > 0.75 and Kappa value > 0.80 show a good agreement between two observers. Multivariable logistic regression analysis was then applied using clinical and CT features that differed significantly between both groups to select independent predictors in discriminating between nonabsorbable inflammatory and malignant SSNs. The final regression model was selected using the forward condition method, and the area under the receiver operating characteristic curve (AUC), accuracy, sensitivity, and specificity were used to evaluate diagnostic performance. The agreement among two observers was fairly good for all CT features. The ICCs values of diagnostic tests for tumour size and CT value of GGO areas were 0.975 and 0.985 (all P < 0.001), respectively. Additionally, Kappa values for distribution, shape, margin, density, target sign, bubble lucency, air bronchogram, intra-nodular vessel sign, abnormal intra-nodular vessel sign, thickened interlobular septa, and pleural changes between two observers were 1.00, 0. Table 1 shows the clinical characteristics of patients in both groups. Malignant SSNs were more frequently observed in women and non-smokers, whereas respiratory symptoms were more common in patients with inflammatory SSNs (all P < 0.05). However, no significant difference in age was observed between the two groups (P > 0.05). Table 2 illustrates the CT features of the observed SSNs in both groups. The median size was 11.00 (9.00, 14.00) mm and 10.00 (9.00, 13.00) mm in inflammatory and malignant SSNs, respectively, and no significant difference was found between the two groups (P > 0.05). Distribution of upper lobe, regular shape, and well-defined margin were more frequently observed in the malignant group than those in the inflammatory group (all P < 0.05). With regard to intranodular and extra-nodular characteristics, malignant SSNs had a significantly higher frequency of pGGNs, whereas inflammatory SSNs had a significantly higher occurrence rate of target sign, abnormal intra-nodular vessel sign, and thickened interlobular septa (all P < 0.05). Furthermore, the CT value of GGO areas in malignant SSNs was higher than that in inflammatory ones (P < 0.001). The cutoff CT value of GGO areas for differentiating inflammatory from malignant SSNs was −657 Hu, with an AUC, sensitivity, and specificity of 0.711, 71.30%, and 62.90%, respectively. However, no significant differences were observed in bubble lucency, air bronchogram, intra-nodular vessel sign, and pleural changes between the two groups (all P > 0.05). )) obtained an AUC (95% CI), accuracy, sensitivity, and specificity of 0.843 (95% CI: 0.811-0.872), 89.76%, 72.86%, and 81.23%, respectively. The internal validation cohort obtained an AUC (95% CI), accuracy, sensitivity, and specificity of 0.830 (95% CI: 0.759-0.887), 83.56%, 73.91%, and 76.42%, respectively. After surgery, CT images of inflammatory and malignant SSNs were correlated with pathological findings. For inflammatory SSNs, GGO was found to correspond to slight interstitial fibrous tissue hyperplasia with inflammatory cell infiltration with or without exudation filling in the alveolar space, while solid density correlated to granuloma formation, organization in alveolar space, or obvious interstitial fiber hyperplasia with inflammatory cell infiltration ( Figure 2 ). For malignant SSNs, GGO was found to be related to the invasion of tumor cells along pre-existing alveolar structures but not completely filling the alveolar space, while the solid component was associated with minimal invasion of tumor cells, alveolar collapse, and fibrosis ( Figure 3 ). Abnormal intra-nodular vessel sign in inflammatory SSNs corresponded to thickening of vessel walls with slight proliferation of fibrous tissue and exudation of alveolar space surrounding vessels (Figure 4) , while that in malignant SSNs corresponded to the proliferation of fibrous tissue surrounding vessels with or without invasion of tumor cells ( Figure 5 ). For patients with SSNs, an accurate preoperative diagnosis can help clinicians select the optimal therapeutic strategy and avoid unnecessary surgical resection of nonabsorbable inflammatory SSNs. In this study, we first compared the clinical and CT features of 70 patients with nonabsorbable inflammatory SSNs and 506 patients with malignant SSNs. We then developed and validated a multivariable logistic regression model for differentiating nonabsorbable inflammatory and For clinical characteristics, this study showed that no significant difference was observed in patient's age between the two groups. Previous research has shown that patients with lung cancer tend to be older than those with inflammatory lesions, possibly because increasing age comes with increasing incidence of malignancy due to decreased immune function and added total exposure to carcinogens; 16, 17 however, those results are not consistent with our findings. The conflicting results among studies may be related to differences in sample size and study design. Furthermore, we found that patients with malignant SSNs were predominantly women and non-smokers. Arenberg 18 indicated that indolent lung adenocarcinoma that predominantly manifested as SSN was more common in non-smoking women, similar to our results. Moreover, this study found that more patients with nonabsorbable inflammatory SSNs had respiratory symptoms, mainly including cough, sputum, and chest pain. Choi et al 19 reported that the presence of respiratory symptoms was associated with benign diseases, mainly including inflammatory lesions and tuberculosis, which corroborated our results. For CT features, we found that malignant SSNs were predominantly located in the upper lobe, which was consistent with the findings of some previous studies. 16, 20 Our results indicated that, compared with inflammatory nodules, more malignant nodules had a regular shape, well-defined margin, and pGGN performance with a greater CT value of GGO areas. These findings could likely be attributed to differences in the pathological basis between inflammatory and malignant SSNs. Pathologically, GGO in inflammatory SSNs was associated with exudation filling in the alveolar space, which tended to result in an irregular shape, vague edge, and a low density of GGO areas on CT. 17, 21 Contrastingly, GGO in malignant SSNs was related to minimal invasion of tumor cells along pre-existing alveolar structures but not completely filling the alveolar space, which might easily cause a regular shape, clear edge, and a relatively high density of GGO areas on CT. Previous research found that a regular shape and a well-defined-but-coarse interface were more common in malignant nodules than those in inflammatory ones, 22,23 with our results showing the same. Additionally, Yu et al 24 found that the possibility of malignancy is significantly increased with a high CT value, and that surgical treatment should thus be considered in those cases. The current study revealed an interesting CT sign, namely, the target sign, which has not been described previously. This pattern was commonly detected in inflammatory nodules and manifested as a focal and round-like solid component in the center of GGO-predominant nodules, similar to a target. This finding may be a consequence of granuloma DovePress formation in the central area, slight interstitial fibrous tissue hyperplasia with inflammatory cell infiltration with or without exudation of alveoli in the peripheral area. Unlike inflammatory PSNs, we found that the solid component in malignant PSNs predominantly appeared as an irregular shape with a scattered or eccentric distribution, which was consistent with previous results. [25] [26] [27] [28] We also found that the intra-nodular vessel sign which could be evaluated only in SSNs was frequently observed in both groups, which was in agreement with the findings of Gao et al 3 showed that intra-nodular vessels can be observed in both inflammatory and malignant nodules. Furthermore, Gao et al 3, 30 indicated that abnormal changes of the blood vessels passing through the nodules are highly suggestive of malignancy. However, we found that abnormal intra-nodular vessel sign was more common in inflammatory SSNs, which differs from the findings of some scholars. The reason for this result may be that previous studies primarily focused on invasive adenocarcinoma, for which more blood supply is needed to maintain the rapid growth of tumor cells. Thus, tumor cells will produce abundant vascular endothelial growth factor to promote angiogenesis, leading to an increase in neovascularization within tumors and concomitant vascular abnormalities. 31, 32 In the present study, all patients had AIS and MIA, which were considered to have a slow growth rate, and angiogenesis in this stage was less obvious compared with that of invasive adenocarcinoma; thus, the incidence of abnormal intra-nodular vessel sign was accordingly lower. For inflammatory nodules, inflammatory mediators may cause abnormal changes, such as vasodilation and hyperpermeability, resulting in a higher percentage of abnormal intra-nodular vessel sign. [33] [34] [35] Therefore, the frequencies of abnormal intra-nodular vessel sign are distinctive among tumors with different invasiveness, suggesting that this sign should be analyzed separately according to the proportion of solid components on CT, which is closely related to tumor invasiveness. Our study indicated that interlobular septal thickening is a frequent manifestation around inflammatory SSNs. Chung et al 36 reported that common diseases, such as pulmonary edema, pneumonias, and pulmonary fibrosis, cause interlobular septal thickening. For inflammatory nodules, this sign may be attributed to inflammation affecting the septa. Multiparametric analysis indicated that presence of respiratory symptoms, distribution of middle/lower lobe, PSNs, irregular shape, CT value of GGO areas < −657 Hu, presence of abnormal intra-nodular vessel sign, and interlobular septal thickening were the most effective factors for diagnosing nonabsorbable inflammatory SSNs. This model obtained an AUC and accuracy of 0.843 and 89.76%, respectively. The internal validation cohort also indicated a good performance. Familiarity with these differential features may improve diagnostic accuracy of SSNs with a solid component ≤5 mm. There were several limitations to this study. First, given that most inflammatory nodules are absorbable and all the patients included in the current study underwent surgical resection, there may be some selection bias. Second, the number of patients with nonabsorbable inflammatory SSNs was small. Third, the CT features of nodules are easily affected by CT acquisition and reconstruction so that the results need to be verified in future studies. Forth, exact matching of the histopathological slice to the imaging slice was very difficult, and further correlation studies with a larger sample size are warranted. In conclusion, our findings demonstrate that nonabsorbable inflammatory and malignant SSNs have different clinical and radiological characteristics. Specifically, SSNs with respiratory symptom, a middle/lower distribution, irregular shape, PSNs density, CT value of GGO areas <−657 Hu, abnormal intra-nodular vascular, and interlobular septal thickening are high suggestive of nonabsorbable inflammatory nodule and follow-up CT should be considered first. A good understanding of these features and pathological basis can contribute to the accurate diagnosis of SSNs, reduction in the number of unnecessary surgeries, and advancements in determining an optimal treatment strategy. SSNs, subsolid nodules; CT, computed tomography; pGGNs, pure ground-glass opacity nodules; PSNs, part-solid nodules; GGO, ground-glass opacity; AIS, adenocarcinoma in situ; MIA, minimally invasive adenocarcinoma; ROC, receiver operating characteristic; AUC, area under the curve; OR, odds ratio; CI, confidence interval. 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The authors report no conflicts of interest in this work. Data supporting the results reported in the manuscript can be found by contacting the correspondence author with email (liqi89011721@163.com). The First Affiliated Hospital of ChongQing Medical University's institutional review board approved this study (approval number: 2019-062), and the need for informed consent was waived due to its retrospective nature. The details of any images, recordings, and figures can be published, and the persons providing consent have been shown the article contents to be published. All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.